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Sequences

Sequence NM_198265

CCGCTCAGAAAGCGCGGGAAAGGCACGCAGCCACGCCCCAAGGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCCCTCATGGCCT TTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTCCCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAAC TGGGGGAAGCCGCCTGGCCTCCAGGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGATCAAAAGTGATTCACCAAAATCA GCTCAAAAATTTTCTCTAATCCTTAAAATATTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACATATATTACACTGACA GTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATTTCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATC AAAAGGTTTAATTGCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGCTGTTGCTGTGCCATCGAATATT CAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGTATTAATTGTAGAAAAAGATGCAACATTTCAGCGGCTCCTAGATGACAACTTTTGCAACAAAT TGTCTCCTTGCATCATGATTACGGGAAAGGGAGTTCCTGATCTAAACACAAGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTGTTTTCAC TCTTGTAGATGCTGATCCACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTCTATGTCTTTTGAAGCTCATCATCTCACAGTTCCAGCT ATTAGATGGCTTGGTCTTCTCCCTTCTGATCTTAAAAGATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGACA GTATCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAATAATGGCAGACTCTAAAATGAAGGCAGAAATTCAAGCTTTGAC TTTCCTATCATCAGATTATCTTTCCAGAGTGTACTTACCTAACAAATTAAAATTTGGAGGATGGATATAAAAATAAATCAGAAGAACTTCTGATTGCCAG AGGCTTTTCATTAGTTTTGTTTTGATTGGCAAATACTATTGTGGAAAGAACATATATTATATTCTTAATTCTGTAAAAGTGAAATAAAATAACTTTCCGT TAATTATATATTTTTGTCAAAACAAATGCTGTACTCCAATTTTCTTTGCAAGGCCTTATTCTTGCCTCTATAGAGACAGATTTCTGTCCTATCTTCTAAA GCAAATTATAAAAGAATATGTTATTTTGACCTTTAAATTATTTTTGAAAAAATAATATTTTATACATGTCATCAAAGTCTACAAAATATTTACCTTCTAC GATACAACTAATGTTAACGCATAAAGTATCTTACTGGTAACAAAAATCATAATGATCTGAATTTGAGATGTTGCAAATGAATTGTGGTGTCCGGTAGTTT CTTCTTACATTTTCCTTTGCCTTTATACTTTAGGGGTCTTACTCCATTAATTCATTTGTTACATTAGTAAAATTCAGTATGAATAAATATTTGGATTGAT GTA

refseq_SPO11.F2 (forward primer)
refseq_SPO11.R2 (reverse primer)
Single exon skipping

Frame 0

PLRKRGKGTQPRPKGAA*DRGFWSFWQPSALMA FAPMGPEASFFDVLDRHRESLLAALRRGGREPPT GGSRLASRFEDSVGLQMVSHCTTRKIKSDSPKS AQKFSLILKILSMIYKLVQSNTYATKRDIYYTD SQLFGNQTVVDNIINDISCMLKVSRRSLHILSTS KGLIAGNLRYIEEDGTKVNCTCGATAVAVPSNI QGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNK LSPCIMITGKGVPDLNTRLLVKKLWDTFHVPVFT LVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPA IRWLGLLPSDLKRLNVPKDSLIPLTKRDQMKLD SILRRPYVTCQPFWRKEMEIMADSKMKAEIQALT FLSSDYLSRVYLPNKLKFGGWI*K*IRRTSDCQ RLFISFVLIGKYYCGKNIYYILNSVKVK*NNFP LIIYFCQNKCCTPIFFARPYSCLYRDRFLSYLLK QIIKEYVILTFKLFLKK*YFIHVIKVYKIFTFY DTTNVNA*SILLVTKIIMI*I*DVANELWCPVV SSYIFLCLYTLGVLLH*FICYISKIQYE*IFGLM

Frame 1

RSESAGKARSHAPRAQPRTGASGASGSRLPSWP LHLWGPRPRSSTFWTDTGSPCWLP*GEVAGSPQ LGEAAWPPGLKILWVFRWYPIAPPERSKVIHQNQ LKNFL*SLKYCP*FIN*YRATLMQPKGTYITLT VNSLVTRLSSTILSMTFLAC*KCQGGVYIYYLH QKV*LLAT*DTSRKMAPK*IVPVVQRLLLCHRIF KEFGI*LQMQSLY*L*KKMQHFSGS*MTTFATN CLLAS*LREREFLI*TQDF*SRNCGIHFMFLFS LL*MLIHMA*K*CASISMDLCLCLLKLIISQFQL LDGLVFSLLILKD*MYLKIV*FH*QKGTK*NLT VS*GDLMLPANHFGEKKWK*WQTLK*RQKFKL* LSYHQIIFPECTYLTN*NLEDGYKNKSEELLIAR GFSLVLF*LANTIVERTYIIFLIL*K*NKITFR *LYIFVKTNAVLQFSLQGLILASIETDFCPIF* SKL*KNMLF*PLNYF*KNNILYMSSKSTKYLPST IQLMLTHKVSYW*QKS**SEFEMLQMNCGVR*F LLTFSFAFIL*GSYSINSFVTLVKFSMNKYLD* C

Frame 2

AQKARERHAATPQGRSLGQGLLELLAAVCPHGL CTYGARGLVLRRFGPTQGVPAGCPEERWQGAPN WGKPPGLQV*RFCGSSDGIPLHHQKDQK*FTKI SSKIFSNP*NIVHDL*ISTEQHLCNQKGHILH*Q STLW*PDCRRQYYQ*HFLHVKSVKEESTYIIYI KRFNCWQLKIHRGRWHQSELYLWCNGCCCAIEY SRNSEFSYRCKVCINCRKRCNISAAPR*QLLQQI VSLHHDYGKGSS*SKHKTFSQETVGYISCSCFH SCRC*STWHRNNVHL*VWIYVYVF*SSSSHSSS Y*MAWSSPF*S*KIKCT*R*FDSTDKKGPNET*Q YPEETLCYLPTILEKRNGNNGRL*NEGRNSSFD FPIIRLSFQSVLT*QIKIWRMDIKINQKNF*LP EAFH*FCFDWQILLWKEHILYS*FCKSEIK*LSV NYIFLSKQMLYSNFLCKALFLPL*RQISVLSSK ANYKRICYFDL*IIFEKIIFYTCHQSLQNIYLL RYN*C*RIKYLTGNKNHNDLNLRCCK*IVVSGSF FLHFPLPLYFRGLTPLIHLLH**NSV*INIWID V

Sequence NM_012444

CCGCTCAGAAAGCGCGGGAAAGGCACGCAGCCACGCCCCAAGGGCGCAGCCTAGGACAGGGGCTTCTGGAGCTTCTGGCAGCCGTCTGCCCTCATGGCCT TTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTCCCTGCTGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAAC TGGGGGAAGCCGCCTGGCCTCCAGTTCTGAGGTTCTTGCATCTATAGAAAATATTATCCAAGACATAATCACAAGCTTGGCAAGAAATGAAGCACCTGCA TTCACGATAGACAACAGATCAAGCTGGGAAAACATAAAGTTTGAAGATTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGATCAAAAGTG ATTCACCAAAATCAGCTCAAAAATTTTCTCTAATCCTTAAAATATTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAACCAAAAGGGACAT ATATTACACTGACAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAATGACATTTCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACAT ATATTATCTACATCAAAAGGTTTAATTGCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGCTGTTGCTG TGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGTATTAATTGTAGAAAAAGATGCAACATTTCAGCGGCTCCTAGATGACAA CTTTTGCAACAAATTGTCTCCTTGCATCATGATTACGGGAAAGGGAGTTCCTGATCTAAACACAAGACTTTTAGTCAAGAAACTGTGGGATACATTTCAT GTTCCTGTTTTCACTCTTGTAGATGCTGATCCACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTCTATGTCTTTTGAAGCTCATCATC TCACAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTTAAAAGATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCA AATGAAACTTGACAGTATCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAATAATGGCAGACTCTAAAATGAAGGCAGAA ATTCAAGCTTTGACTTTCCTATCATCAGATTATCTTTCCAGAGTGTACTTACCTAACAAATTAAAATTTGGAGGATGGATATAAAAATAAATCAGAAGAA CTTCTGATTGCCAGAGGCTTTTCATTAGTTTTGTTTTGATTGGCAAATACTATTGTGGAAAGAACATATATTATATTCTTAATTCTGTAAAAGTGAAATA AAATAACTTTCCGTTAATTATATATTTTTGTCAAAACAAATGCTGTACTCCAATTTTCTTTGCAAGGCCTTATTCTTGCCTCTATAGAGACAGATTTCTG TCCTATCTTCTAAAGCAAATTATAAAAGAATATGTTATTTTGACCTTTAAATTATTTTTGAAAAAATAATATTTTATACATGTCATCAAAGTCTACAAAA TATTTACCTTCTACGATACAACTAATGTTAACGCATAAAGTATCTTACTGGTAACAAAAATCATAATGATCTGAATTTGAGATGTTGCAAATGAATTGTG GTGTCCGGTAGTTTCTTCTTACATTTTCCTTTGCCTTTATACTTTAGGGGTCTTACTCCATTAATTCATTTGTTACATTAGTAAAATTCAGTATGAATAA ATATTTGGATTGATGTA

refseq_SPO11.F2 (forward primer)
refseq_SPO11.R2 (reverse primer)
Single exon skipping

Frame 0

PLRKRGKGTQPRPKGAA*DRGFWSFWQPSALMA FAPMGPEASFFDVLDRHRESLLAALRRGGREPPT GGSRLASSSEVLASIENIIQDIITSLARNEAPA FTIDNRSSWENIKFEDSVGLQMVSHCTTRKIKS DSPKSAQKFSLILKILSMIYKLVQSNTYATKRDI YYTDSQLFGNQTVVDNIINDISCMLKVSRRSLH ILSTSKGLIAGNLRYIEEDGTKVNCTCGATAVA VPSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDN FCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFH VPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHH LTVPAIRWLGLLPSDLKRLNVPKDSLIPLTKRDQ MKLDSILRRPYVTCQPFWRKEMEIMADSKMKAE IQALTFLSSDYLSRVYLPNKLKFGGWI*K*IRR TSDCQRLFISFVLIGKYYCGKNIYYILNSVKVK* NNFPLIIYFCQNKCCTPIFFARPYSCLYRDRFL SYLLKQIIKEYVILTFKLFLKK*YFIHVIKVYK IFTFYDTTNVNA*SILLVTKIIMI*I*DVANELW CPVVSSYIFLCLYTLGVLLH*FICYISKIQYE* IFGLM

Frame 1

RSESAGKARSHAPRAQPRTGASGASGSRLPSWP LHLWGPRPRSSTFWTDTGSPCWLP*GEVAGSPQ LGEAAWPPVLRFLHL*KILSKT*SQAWQEMKHLH SR*TTDQAGKT*SLKILWVFRWYPIAPPERSKV IHQNQLKNFL*SLKYCP*FIN*YRATLMQPKGT YITLTVNSLVTRLSSTILSMTFLAC*KCQGGVYI YYLHQKV*LLAT*DTSRKMAPK*IVPVVQRLLL CHRIFKEFGI*LQMQSLY*L*KKMQHFSGS*MT TFATNCLLAS*LREREFLI*TQDF*SRNCGIHFM FLFSLL*MLIHMA*K*CASISMDLCLCLLKLII SQFQLLDGLVFSLLILKD*MYLKIV*FH*QKGT K*NLTVS*GDLMLPANHFGEKKWK*WQTLK*RQK FKL*LSYHQIIFPECTYLTN*NLEDGYKNKSEE LLIARGFSLVLF*LANTIVERTYIIFLIL*K*N KITFR*LYIFVKTNAVLQFSLQGLILASIETDFC PIF*SKL*KNMLF*PLNYF*KNNILYMSSKSTK YLPSTIQLMLTHKVSYW*QKS**SEFEMLQMNC GVR*FLLTFSFAFIL*GSYSINSFVTLVKFSMNK YLD*C

Frame 2

AQKARERHAATPQGRSLGQGLLELLAAVCPHGL CTYGARGLVLRRFGPTQGVPAGCPEERWQGAPN WGKPPGLQF*GSCIYRKYYPRHNHKLGKK*STC IHDRQQIKLGKHKV*RFCGSSDGIPLHHQKDQK* FTKISSKIFSNP*NIVHDL*ISTEQHLCNQKGH ILH*QSTLW*PDCRRQYYQ*HFLHVKSVKEEST YIIYIKRFNCWQLKIHRGRWHQSELYLWCNGCCC AIEYSRNSEFSYRCKVCINCRKRCNISAAPR*Q LLQQIVSLHHDYGKGSS*SKHKTFSQETVGYIS CSCFHSCRC*STWHRNNVHL*VWIYVYVF*SSSS HSSSY*MAWSSPF*S*KIKCT*R*FDSTDKKGP NET*QYPEETLCYLPTILEKRNGNNGRL*NEGR NSSFDFPIIRLSFQSVLT*QIKIWRMDIKINQKN F*LPEAFH*FCFDWQILLWKEHILYS*FCKSEI K*LSVNYIFLSKQMLYSNFLCKALFLPL*RQIS VLSSKANYKRICYFDL*IIFEKIIFYTCHQSLQN IYLLRYN*C*RIKYLTGNKNHNDLNLRCCK*IV VSGSFFLHFPLPLYFRGLTPLIHLLH**NSV*I NIWIDV