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Sequences

Sequence NM_021972

GCTCCGCGGCGCCGGCTGCGAAGTTGAGCGAAAAGTTTGAGGCCGGAGGGAGCGAGGCCGGGGAGTCCGCTCCAGCGGGGCGCTCCAGTCCCTCAGACGT GGGCTGAGCTTGGGACGAGCTGCGTTCCGCCCCAGGCCACTGTAGGGAACGGCGGTGGCGCCTCCCCAGCAAACCGGACCGACTGGGTCCAGCCGCCGCA GGGAATGACGCCGGTGCTCCTGCAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCCTGCGACGCCCGCCTGGGCAGCACCGATAAGGAGC TGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGCGCCAGGGACCCCCTGGCAGCGGGAGCCGCGGGTCGAGGTTATGGATCCAGTGGTCGGTTG CGGACGTGGCCTCTTTGGTTTTGTTTTCTCAGCGGGCGGCCCCCGGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGC AAGGGCAAGGCCTTGCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACTGAGCGGCGGAACCACG CGCGGGAGCTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGA GCGGCCTGACTGGGAGACCGCCATCCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCTGGCTAT GAGCAGGTCACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGTCACCCATGAACCTGCTGTCTCTGCACACGGCTTCGG GGCTGCGCCTCTTCTCTGTGCTCAGCCTGGCCTGGGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCAC TCTGGGCACCTTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTTCCAAGACACCTGCCTCCCCC GTTGTGGTCCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAGGAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAG TCCTGGCACTGCTGCACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGTTCTACGTGCGGGCGGG AGTGTCTCGTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCATATGGAGTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCC TTCCGCTTGGAGCCCAAGGATGGGAAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCT GGATGGTCAGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAGCCCTTATGACCCCTGGGCCGCGCTGTGCCTTA GTGTCTACTTGCAGGACCCTTCCTCCTTCCCTAGGGCTGCAGGGCCTGTCCACAGCTCCTGTGGGGGTGGAGGAGACTCCTCTGGAGAAGGGTGAGAAGG TGGAGGCTATGCTTTGGGGGGACAGGCCAGAATGAAGTCCTGGGTCAGGAGCCCAGCTGGCTGGGCCCAGCTGCCTATGTAAGGCCTTCTAGTTTGTTCT GAGACCCCCACCCCACGAACCAAATCCAAATAAAGTGACATTCCCAGCCTG

refseq_SPHK1.F1 (forward primer)
refseq_SPHK1.R1 (reverse primer)
Alternative donor

Frame 0

APRRRLRS*AKSLRPEGARPGSPLQRGAPVPQT WAELGTSCVPPQATVGNGGGASPANRTDWVQPPQ GMTPVLLQPRLRAGKASPTAGPATPAWAAPIRS *RQEPPPRAAPPQRQGPPGSGSRGSRLWIQWSV ADVASLVLFSQRAAPGACSRGPAACWCC*TRAAA RARPCSSSGVTCSPFWLRLKSPSR*CSLSGGTT RGSWCGRRSWAAGTLWWSCLETG*CTRW*TGSW SGLTGRPPSRSPCVASQQALATRWQLP*TIMLAM SRSPMKTS*PTARYCCAAGCCHP*TCCLCTRLR GCASSLCSAWPGASLLMWT*RVRSIGVWGRCAS LWAPSCVWQPCAPTAADWPTSL*EEWVPRHLPPP LWSSRAR*MHTLCHWRSQCPLTGQWCPTRTLC* SWHCCTRTWAVRCLLHPWAAVQLASCICSTCGR ECLVPCCCASSWPWRRAGIWSMNAPTWYMCPWSP SAWSPRMGKVCLQWMGN*WLARPCRARCTQTTS GWSAVAWSPRPAGSPSRCHRQKSPYDPWAALCL SVYLQDPSSFPRAAGPVHSSCGGGGDSSGEG*EG GGYALGGQARMKSWVRSPAGWAQLPM*GLLVCS ETPTPRTKSK*SDIPSL

Frame 1

LRGAGCEVERKV*GRRERGRGVRSSGALQSLRR GLSLGRAAFRPRPL*GTAVAPPQQTGPTGSSRR RE*RRCSCSHGSGRGRRAPQPALRRPPGQHR*GA EGRSRRHGQRPHSARDPLAAGAAGRGYGSSGRL RTWPLWFCFLSGRPPGRAPAALPRAGAAEPARR QGQGLAALPESRAAPFG*G*NLLHADAH*AAEPR AGAGAVGGAGPLGRSGGHVWRRADARGGERAHG AA*LGDRHPEAPV*PPSRLWQRAGSFLEPLCWL *AGHQ*RPPDQLHAIAVPPAAVTHEPAVSAHGFG AAPLLCAQPGLGLHC*CGPRE*EVSASGGDALH SGHLPASGSPAHLPRPTGLPPCRKSGFQDTCLP RCGPAGPGRCTPCATGGASALSLDSGARRGLCAS PGTAALAPGQ*DVCCTHGPLCSWRHASVLRAGG SVSCHAAAPLPGHGEGQAYGV*MPLLGICARGR LPLGAQGWERCVCSGWGIDG*RGRAGPGAPKLLL DGQRLRGAPAQLEAPADATARRALMTPGPRCAL VSTCRTLPPSLGLQGLSTAPVGVEETPLEKGEK VEAMLWGDRPE*SPGSGAQLAGPSCLCKAF*FVL RPPPHEPNPNKVTFPA

Frame 2

SAAPAAKLSEKFEAGGSEAGESAPAGRSSPSDV G*AWDELRSAPGHCRERRWRLPSKPDRLGPAAA GNDAGAPAATAPGGEGEPHSRPCDARLGSTDKE LKAGAAATGSAPTAPGTPWQREPRVEVMDPVVGC GRGLFGFVFSAGGPRGVLPRPCRVLVLLNPRGG KGKALQLFRSHVQPLLAEAEISFTLMLTERRNH ARELVRSEELGRWDALVVMSGDGLMHEVVNGLME RPDWETAIQKPLCSLPAGSGNALAASLNHYAGY EQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTAS GLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFT LGTFLRLAALRTYRGRLAYLPVGRVGSKTPASP VVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVL VLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAG VSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVA FRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYF WMVSGCVEPPPSWKPQQMPPPEEPL*PLGRAVP* CLLAGPFLLP*GCRACPQLLWGWRRLLWRRVRR WRLCFGGTGQNEVLGQEPSWLGPAAYVRPSSLF *DPHPTNQIQIK*HSQP

Sequence NM_182965

GAAAAGTTTGAGGCCGGAGGGAGCGAGGCCGGGGAGTCCGCTCCAGCGGGGCGCTCCAGTCCCTCAGACGTGGGCTGAGCTTGGGACGAGCTGCGTTCCG CCCCAGGCCACTGTAGGGAACGGCGGTGGCGCCTCCCCAGCAAACCGGACCGACTGGGTAGGGCCGCCCACCCTGCCTTCGCGCCGCTCGTGGCTCCTGT GCGGCCCGCCCTCGCGGGGCCCCGGGAACTGGGCCACTTGTCGCTTGGGCGAGAGCAGGCGGCAGCTGGTGGCCCGGTTCGGGCTTGGCTTTGGCGCGAC CCGGGAGCGGCTCCCACGAGCGCCGCGCGCGTCGCAACGGAGCGGGGCCCTGAGAAGCGCGCGCCGCGGCTCCCACCGCTCTGGAGCTCCGGGCAGGGGA CACGGCAACCTGGATGGCTGGGGCAGGGATCCTCTCCCAGAACTTCGTGCCCAGCAATGTCCGCTCAAGTTCTGGGATTTTTACGCAGCTGGACTCCCCT CCCCCTGGCAGCCCCGAGGGGTCCAGCCGCCGCAGGGAATGACGCCGGTGCTCCTGCAGCCACGGCTCCGGGCGGGGAAGGCGAGCCCCACAGCCGGCCC TGCGACGCCCGCCTGGGCAGCACCGATAAGGAGCTGAAGGCAGGAGCCGCCGCCACGGGCAGCGCCCCCACAGCGCCAGGGACCCCCTGGCAGCGGGAGC CGCGGGTCGAGGTTATGGATCCAGCGGGCGGCCCCCGGGGCGTGCTCCCGCGGCCCTGCCGCGTGCTGGTGCTGCTGAACCCGCGCGGCGGCAAGGGCAA GGCCTTGCAGCTCTTCCGGAGTCACGTGCAGCCCCTTTTGGCTGAGGCTGAAATCTCCTTCACGCTGATGCTCACTGAGCGGCGGAACCACGCGCGGGAG CTGGTGCGGTCGGAGGAGCTGGGCCGCTGGGACGCTCTGGTGGTCATGTCTGGAGACGGGCTGATGCACGAGGTGGTGAACGGGCTCATGGAGCGGCCTG ACTGGGAGACCGCCATCCAGAAGCCCCTGTGTAGCCTCCCAGCAGGCTCTGGCAACGCGCTGGCAGCTTCCTTGAACCATTATGCTGGCTATGAGCAGGT CACCAATGAAGACCTCCTGACCAACTGCACGCTATTGCTGTGCCGCCGGCTGCTGTCACCCATGAACCTGCTGTCTCTGCACACGGCTTCGGGGCTGCGC CTCTTCTCTGTGCTCAGCCTGGCCTGGGGCTTCATTGCTGATGTGGACCTAGAGAGTGAGAAGTATCGGCGTCTGGGGGAGATGCGCTTCACTCTGGGCA CCTTCCTGCGTCTGGCAGCCCTGCGCACCTACCGCGGCCGACTGGCCTACCTCCCTGTAGGAAGAGTGGGTTCCAAGACACCTGCCTCCCCCGTTGTGGT CCAGCAGGGCCCGGTAGATGCACACCTTGTGCCACTGGAGGAGCCAGTGCCCTCTCACTGGACAGTGGTGCCCGACGAGGACTTTGTGCTAGTCCTGGCA CTGCTGCACTCGCACCTGGGCAGTGAGATGTTTGCTGCACCCATGGGCCGCTGTGCAGCTGGCGTCATGCATCTGTTCTACGTGCGGGCGGGAGTGTCTC GTGCCATGCTGCTGCGCCTCTTCCTGGCCATGGAGAAGGGCAGGCATATGGAGTATGAATGCCCCTACTTGGTATATGTGCCCGTGGTCGCCTTCCGCTT GGAGCCCAAGGATGGGAAAGGTGTGTTTGCAGTGGATGGGGAATTGATGGTTAGCGAGGCCGTGCAGGGCCAGGTGCACCCAAACTACTTCTGGATGGTC AGCGGTTGCGTGGAGCCCCCGCCCAGCTGGAAGCCCCAGCAGATGCCACCGCCAGAAGAGCCCTTATGACCCCTGGGCCGCGCTGTGCCTTAGTGTCTAC TTGCAGGACCCTTCCTCCTTCCCTAGGGCTGCAGGGCCTGTCCACAGCTCCTGTGGGGGTGGAGGAGACTCCTCTGGAGAAGGGTGAGAAGGTGGAGGCT ATGCTTTGGGGGGACAGGCCAGAATGAAGTCCTGGGTCAGGAGCCCAGCTGGCTGGGCCCAGCTGCCTATGTAAGGCCTTCTAGTTTGTTCTGAGACCCC CACCCCACGAACCAAATCCAAATAAAGTGACATTCCCAGCCTG

refseq_SPHK1.F1 (forward primer)
refseq_SPHK1.R1 (reverse primer)
Alternative donor

Frame 0

EKFEAGGSEAGESAPAGRSSPSDVG*AWDELRS APGHCRERRWRLPSKPDRLGRAAHPAFAPLVAPV RPALAGPRELGHLSLGREQAAAGGPVRAWLWRD PGAAPTSAARVATERGPEKRAPRLPPLWSSGQG TRQPGWLGQGSSPRTSCPAMSAQVLGFLRSWTPL PLAAPRGPAAAGNDAGAPAATAPGGEGEPHSRP CDARLGSTDKELKAGAAATGSAPTAPGTPWQRE PRVEVMDPAGGPRGVLPRPCRVLVLLNPRGGKGK ALQLFRSHVQPLLAEAEISFTLMLTERRNHARE LVRSEELGRWDALVVMSGDGLMHEVVNGLMERP DWETAIQKPLCSLPAGSGNALAASLNHYAGYEQV TNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLR LFSVLSLAWGFIADVDLESEKYRRLGEMRFTLG TFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVV QQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLA LLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVS RAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRL EPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMV SGCVEPPPSWKPQQMPPPEEPL*PLGRAVP*CL LAGPFLLP*GCRACPQLLWGWRRLLWRRVRRWRL CFGGTGQNEVLGQEPSWLGPAAYVRPSSLF*DP HPTNQIQIK*HSQP

Frame 1

KSLRPEGARPGSPLQRGAPVPQTWAELGTSCVP PQATVGNGGGASPANRTDWVGPPTLPSRRSWLL CGPPSRGPGNWATCRLGESRRQLVARFGLGFGAT RERLPRAPRASQRSGALRSARRGSHRSGAPGRG HGNLDGWGRDPLPELRAQQCPLKFWDFYAAGLP SPWQPRGVQPPQGMTPVLLQPRLRAGKASPTAGP ATPAWAAPIRS*RQEPPPRAAPPQRQGPPGSGS RGSRLWIQRAAPGACSRGPAACWCC*TRAAARA RPCSSSGVTCSPFWLRLKSPSR*CSLSGGTTRGS WCGRRSWAAGTLWWSCLETG*CTRW*TGSWSGL TGRPPSRSPCVASQQALATRWQLP*TIMLAMSR SPMKTS*PTARYCCAAGCCHP*TCCLCTRLRGCA SSLCSAWPGASLLMWT*RVRSIGVWGRCASLWA PSCVWQPCAPTAADWPTSL*EEWVPRHLPPPLW SSRAR*MHTLCHWRSQCPLTGQWCPTRTLC*SWH CCTRTWAVRCLLHPWAAVQLASCICSTCGRECL VPCCCASSWPWRRAGIWSMNAPTWYMCPWSPSA WSPRMGKVCLQWMGN*WLARPCRARCTQTTSGWS AVAWSPRPAGSPSRCHRQKSPYDPWAALCLSVY LQDPSSFPRAAGPVHSSCGGGGDSSGEG*EGGG YALGGQARMKSWVRSPAGWAQLPM*GLLVCSETP TPRTKSK*SDIPSL

Frame 2

KV*GRRERGRGVRSSGALQSLRRGLSLGRAAFR PRPL*GTAVAPPQQTGPTG*GRPPCLRAARGSC AARPRGAPGTGPLVAWARAGGSWWPGSGLALAR PGSGSHERRARRNGAGP*EARAAAPTALELRAGD TATWMAGAGILSQNFVPSNVRSSSGIFTQLDSP PPGSPEGSSRRRE*RRCSCSHGSGRGRRAPQPA LRRPPGQHR*GAEGRSRRHGQRPHSARDPLAAGA AGRGYGSSGRPPGRAPAALPRAGAAEPARRQGQ GLAALPESRAAPFG*G*NLLHADAH*AAEPRAG AGAVGGAGPLGRSGGHVWRRADARGGERAHGAA* LGDRHPEAPV*PPSRLWQRAGSFLEPLCWL*AG HQ*RPPDQLHAIAVPPAAVTHEPAVSAHGFGAA PLLCAQPGLGLHC*CGPRE*EVSASGGDALHSGH LPASGSPAHLPRPTGLPPCRKSGFQDTCLPRCG PAGPGRCTPCATGGASALSLDSGARRGLCASPG TAALAPGQ*DVCCTHGPLCSWRHASVLRAGGSVS CHAAAPLPGHGEGQAYGV*MPLLGICARGRLPL GAQGWERCVCSGWGIDG*RGRAGPGAPKLLLDG QRLRGAPAQLEAPADATARRALMTPGPRCALVST CRTLPPSLGLQGLSTAPVGVEETPLEKGEKVEA MLWGDRPE*SPGSGAQLAGPSCLCKAF*FVLRP PPHEPNPNKVTFPA