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Sequences

Sequence NM_199436

GGCCCGAGCCACCGACTGCAGGAGGAGAAGGGGTTGTGCTCCTGGCCGAGGAAGGAGAAAGGGGCGGGGCCGGCGGGCAGCGTGCGGCAGTGCGGAGCTC CTGAGACCGGCGGGCACACGGGGGTCTGTGGCCCCCGCCGTAGCAGTGGCTGCCGCCGTCGCTTGGTTCCCGTCGGTCTGCGGGAGGCGGGTTATGGCGG CGGCGGCAGTGAGAGCTGTGAATGAATTCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCCAGGCCTCCGCCCC CTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCCGAGTCGCCGCATAAGCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGG CTTCGCGCTGCTGCGTTTGGTCGCCTTCCACCTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCC GGGGCCGCGCCAGCACCTGCCTCGGCCTCGGCCCCGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCGAGTCTTCCACAAACAGGCCTTCGAGTACA TCTCCATTGCCCTGCGCATCGATGAGGATGAGAAAGCAGGACAGAAGGAGCAAGCTGTGGAATGGTATAAGAAAGGTATTGAAGAACTGGAAAAAGGAAT AGCTGTTATAGTTACAGGACAAGGTGAACAGTGTGAAAGAGCTAGACGCCTTCAAGCTAAAATGATGACTAATTTGGTTATGGCCAAGGACCGCTTACAA CTTCTAGAAAGTGGAGCTGTTCCAAAAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATCTGCAG GCCTTTCAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAGGGATCTGGTCCTGCTCCTACCACTCATAAGGGTAC TCCGAAAACAAATAGGACAAATAAACCTTCTACCCCTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGGACAGCAACCTTGCT AACCTTATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCAAAACAAGCATTGCAAGAAATTGTTA TTCTTCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTCCTGCCAGAGGGCTGTTACTCTTTGGTCCACCTGGGAATGGGAAGACAATGCTGGC TAAAGCAGTAGCTGCAGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTTTAACTTCAAAATACGTGGGAGAAGGAGAGAAATTGGTGAGGGCT CTTTTTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAGATGAAGTTGATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTA GACGCCTAAAAACTGAATTTCTAATAGAATTTGATGGTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAAGAGCT TGATGAGGCTGTTCTCAGGCGTTTCATCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAAGACTACTTTTGCTTAAAAATCTGTTATGTAAACAA GGAAGTCCATTGACCCAAAAAGAACTAGCACAACTTGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGG GTCCTATCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAAAAAT AAAACGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTGGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCTTTGTAAACCTGCAG AACATTTTACTTAAAAGAGGAAACACAAGATCTTCAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGGACTTTTTAGAGTCTTACATATTTG TGCACCAAACTTGAAGATGAACCAGAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTTTTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGT TATCCCAATGGACACTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTACCAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCA GATGAAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACATTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCCTACTTT TCTTTTCTACTGTTGTCTTAACTACAGGTGATTGGAATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCAGTGTGCCAAATGAAAC TTTTTTCCTAAGTAACTGTAATAGGAAAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAACAATTGTTGTGTTCTTTTTACCTTTT ATTTTTCTATTACCTTGCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATGGTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCT GTCATATAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAATGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCTGATG GAATTTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCAATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGAAATACTAGGAACTA AGACATTTCTAATTTATTGCTTATTACTTTCTTAATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTTATTCTTAATAATTATTAATCCCTT CAATGAAACTTTAAAAAAACTGAATTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGCTTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTT GATCTGCCTTTTGTTCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATATGCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGAC TCATATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATTTGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGGTGCTTA AATGATGCTATGTAAAATGTCATGAGTGGAAAGAATATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGTTTTGAAAGAATACATT AAAATAAAAAACTTGCCCCTACTAGGTAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGCTCTTGTTAAAGATTTGTTTGAATATA GAAGATGCATGATTTCTGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCAATCTCGACTCACCACAAC CTCCGCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTTAGTA GAGATGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATAA GCCACTGCGCCCAGCCAGAAGATGCATGATTTCTTAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCTCTTCCAATCATGACTTTGGAACTC CCTGAATAATAAAAATGAGAGTTGAGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTATGCATATGTTAGGTGATGGGTAGTATCCCTTTAAG GTCTCAAACATTACAACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAAATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATT TCAGTTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAACGTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCCAG CTCAACTTTTAATATATTGTCTCCTTTAAAACTATCATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAAATGTTAAAAGAGATAA AAGTCAGGTTAATACTATCTTAAACACTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGTCTGAACTTCAGCTAGTGCTAGAGAAC TATTTTCTATGACTTAACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCATGAAAATAGAGTAATGATATAGTAGGAGATAAGGGAT TGGTTTGGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATAATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTGTGTT GTATTACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTGTGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTAAAACTTACTGATAT ATTGGACTTTGAGCCAAGGGAAAGAATGAGTACTATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACAGTCTGTCCATTGAGAATATTAGAT TTCTGACTTGCAAATATGTTTGTACTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAATTTAATTACATATACAGCCGTCTTTGTTTATAGTG TAGAATTCTTTATATTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTACAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATG CTTGTCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAAATGAATTAAAGGGTACAGTTGCATAAAGTGGGTTTTTATCCTAATGTATTGGAA ATAAATGATAAACTTTA

refseq_SPAST.F1 (forward primer)
refseq_SPAST.R1 (reverse primer)
Single exon skipping

Frame 0

GPSHRLQEEKGLCSWPRKEKGAGPAGSVRQCGA PETGGHTGVCGPRRSSGCRRRLVPVGLREAGYGG GGSESCE*ILRVDEGRRKAPAAPATRCLPGLRP LAWPPPLPPPGRPLRPSRRISGTCTISPTRCL* ASRCCVWSPSTWGSSSCGSASASPAPSWQPRGAP GPRQHLPRPRPRRRCRAARPSASESSTNRPSST SPLPCASMRMRKQDRRSKLWNGIRKVLKNWKKE *LL*LQDKVNSVKELDAFKLK**LIWLWPRTAYN F*KVELFQKEKTP*HTLVIHCLVQKQL*KLDLQ AFQATIEHLVTVVYPWFLE*NRDLVLLLPLIRV LRKQIGQINLLPLQLLLVRKKT*RILGMWTATLL TL**MKLWTMEQLLNLMI*LVKTWQNKHCKKLL FFLL*GLSCSQGLELLPEGCYSLVHLGMGRQCW LKQ*LQNRMQPSLI*VLQV*LQNTWEKERNW*GL FLLWLENFNLL*FL*MKLIAFCVKEEKGSTMLV DA*KLNF**NLMVYSLLEMTEYL*WVQLIGHKS LMRLFSGVSSNGYMCLYQMRRQDYFCLKICYVNK EVH*PKKN*HNLLE*LMDTQEVT*QLWQKMQHW VLSEN*NQNR*RICLPVR*EIFDYLTSLNP*KK *NAASALKL*KRTYVGTRTLEIPLFKEIPL*TCR TFYLKEETQDLQ*TSSATETA*VYRTF*SLTYL CTKLEDEPENRLKQNIQCKCNFLLFKALP*WSQ LSQWTLS*STTKPDSGLLYQYNHNVNNNLYIVLQ MKVFQEQ*MVEDTRTFVCLSSDVFS*NSNFSYF SFLLLS*LQVIGMPNTLKFIFFFRFINSVCQMK LFS*VTVIGKSLF*EFLLHKSTDIKQLLCSFYLL FFYYLATKQFR*QYNSKKANMVK*RRFEGLSYS VI*HVDQSSCDLQYFFF*CICQKSVVDC*LLPD GIYFLQELF*YLREPILTAVKMFPVQEKGNTRN* DISNLLLITFLILQDNYKQVELPSFILNNY*SL Q*NFKKTEFLYMAYIFLVPSACFINSKVLVLVC *SAFCSPKMYSNSICLYKYAWIFIIKMSL*GVET HIMAF*IL**RQIDICP*FTG*KFVYRTFLWCL NDAM*NVMSGKNICSSNKNFSFRKDFLGFERIH *NKKLAPTR*ELYNEDIHSS*FYSCSC*RFV*I* KMHDFWVFFFF*DRVSLLLPRLECNGAISTHHN LRLPGSSNSPASASRVAGITGMRHYPS*FCIFS RDGVSPCWSGWS*TPDLR*SACLGLPKCWDYRHK PLRPARRCMIS*DHMLFVAIR*IMSLPIMTLEL PE**K*ELR*IGEKKFFSSQSYAYVR*WVVSL* GLKHYNINYEILITKGSNEIYMMKRIEKF*IKTF QLSS*NFIVFI*KINVIDTWITG*SY*RTMWFQ LNF*YIVSFKTIMVIILLGKTFR*QRFQMLKEI KVRLILS*TLSQKIITV*KLLS*SSLNFS*C*RT IFYDLTLTKFYFKLFL**KGHENRVMI**EIRD WFGLFQ*R*KLLKFSELIMT*IVTF*IRC*ALC CITS*KIMLKH*ALVCSSC*YGRVL*TKLKLTDI LDFEPRERMSTIFPDILRVKAYSKTVCPLRILD F*LANMFVLQKN*RKSRY*NSNLITYTAVFVYS VEFFIFCTKTNSFGKMNHLQFGFGL*VKGFSFKC LSQF*SGLLYFSSEEMN*RVQLHKVGFYPNVLE INDKL

Frame 1

ARATDCRRRRGCAPGRGRRKGRGRRAACGSAEL LRPAGTRGSVAPAVAVAAAVAWFPSVCGRRVMA AAAVRAVNEFSGWTREEERLRRRQQPGASQASAP LPGPRPSRRRAGPSARVAA*AEPVLFLLPAVCR LRAAAFGRLPPGAPLRVALPALLPRPHGSQEEL RGRASTCLGLGPGAGAGRRGRARPSLPQTGLRVH LHCPAHR*G*ESRTEGASCGMV*ERY*RTGKRN SCYSYRTR*TV*KS*TPSS*NDD*FGYGQGPLT TSRKWSCSKKKRPLNTH**FTASFKNSYENWICR PFRPP*ST*LQWFIHGFWSETGIWSCSYHS*GY SENK*DK*TFYPYNCYS*EKRLEEF*ECGQQPC *PYNE*NCGQWNSC*I**YSWSRLGKTSIARNCY SSFSEA*VVHRA*SSCQRAVTLWSTWEWEDNAG *SSSCRIECNLL*YKCCKFNFKIRGRRREIGEG SFCCGSRTSTFYNFYR*S**PFV*KKRRGARC** TPKN*ISNRI*WCTVCWR*QSTCNGCN**ATRA **GCSQAFHQTGICVFTK*GDKTTFA*KSVM*T RKSIDPKRTSTTC*ND*WILRK*PNSFGKRCSTG SYPRTKTRTGEEYVCQ*DEKYSII*LH*ILEKN KTQRQPSNFRSVHTLEQGLWRYHCLRKYLCKPA EHFT*KRKHKIFNERHRLQKQPKFTGLFRVLHIC APNLKMNQKTDLNKIYNANVIFCCLRPCLDGHS YPNGH*VRAQQNLILVFFTNIIIM*IIICILCC R*KYSRNSEW*KTQEHLFVCLLMFFLKIVISPTF LFYCCLNYR*LECQTLLSLFSFFVL*IQCAK*N FFPK*L**EKVYFESFFFINLQTLNNCCVLFTF YFSITLLPNSLDSNIIAKKQIW*NREGLKV*VTL SYNM*ISLHVTCSIFFSNVFVRNLL*TVNFFLM EFIFCKNYSDI*ESQF*LL*KCFQCKRREILGT KTFLIYCLLLS*FYRIIISKWNYHLLFLIIINPF NETLKKLNFYTWHTFF*FLLLALLTQKF*F*SV DLPFVLPKCTVIPFVCINMPGFSL*KCHCRE*R LISWPFKYCNKGK*IFALSLLVKSLFTELFSGA* MMLCKMS*VERIFVVVTRIFHLGKIS*VLKEYI KIKNLPLLGKNFIMKTYILLNFTLALVKDLFEY RRCMISGFFFFFETEFRSCCPGWSAMAQSRLTTT SASQVQAILLPQPPE*LGLQACATTPANFVFLV EMGFLHVGQAGLELLTSGDPPASASQSAGITGI SHCAQPEDA*FLRIICCL*P*GKSCLFQS*LWNS LNNKNES*DK*GKKNFFQARAMHMLGDG*YPFK VSNITTSIMKY**RKVVMKYI**KELRSSKLRH FS*AHKISLFSFKRLTLLILG*LANHIKGLCGSS STFNILSPLKLSWL*FYWERLLDNKDFKC*KR* KSG*YYLKH*VRKSLLYRSCFPDQV*TSASARE LFSMT*L*PSFILSCFFDRRAMKIE**YSRR*GI GLVFFNKDRSC*SFLN***LRL*PFRFGVELCV VLLPKR*CLNIKH*CALHVNMAEFCKLN*NLLI YWTLSQGKE*VLSFQIS*G*KLILRQSVH*EY*I SDLQICLYSRRIRGKADTRILI*LHIQPSLFIV *NSLYFVQKLILLVK*TIYSSVLDSESKDFPLN ACLNFSLVFCTFLQKK*IKGYSCIKWVFILMYWK *MINF

Frame 2

PEPPTAGGEGVVLLAEEGERGGAGGQRAAVRSS *DRRAHGGLWPPP*QWLPPSLGSRRSAGGGLWR RRQ*EL*MNSPGGRGKKKGSGGASNPVPPRPPP PCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVG FALLRLVAFHLGLLFVWLCQRFSRALMAAKRSS GAAPAPASASAPAPVPGGEAERVRVFHKQAFEY ISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGI AVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQ LLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSA GLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGT PKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLA NLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIV ILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLA KAVAAESNATFFNISAASLTSKYVGEGEKLVRA LFAVARELQPSIIFIDEVDSLLCERREGEHDAS RRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQEL DEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQ GSPLTQKELAQLARMTDGYSGSDLTALAKDAAL GPIRELKPEQVKNMSASEMRNIRLSDFTESLKKI KRSVSPQTLEAYIRWNKDFGDTTV*GNTFVNLQ NILLKRGNTRSSMNVIGYRNSLSLQDFLESYIF VHQT*R*TRKQT*TKYTMQM*FFVV*GLALMVTV IPMDTKLEHNKT*FWSSLPI*S*CK**FVYCVA DESIPGTVNGRRHKNICLFVF*CFFLK**FLLL FFSTVVLTTGDWNAKHS*VYFLFSFYKFSVPNET FFLSNCNRKKFILRVSSS*IYRH*TIVVFFLPF IFLLPCYQTV*IAI**QKSKYGKIEKV*RFELL CHITCRSVFM*PAVFFFLMYLSEICCRLLTSS*W NLFSARIILIFKRANFNCCENVSSAREGKY*EL RHF*FIAYYFLNFTG*L*ASGTTIFYS**LLIP SMKL*KN*IFIHGIHFSSSFCLLY*LKSSSSSLL ICLLFSQNVQ*FHLFV*ICLDFHYKNVIVGSRD SYHGLLNIVIKANRYLPLVYWLKVCLQNFSLVL K*CYVKCHEWKEYL***QEFFI*ERFLRF*KNTL K*KTCPY*VRTL**RHTFFLILLLLLLKICLNI EDA*FLGFFFFLRQSFALVAQAGVQWRNLDSPQ PPPPRFKQFSCLSLPSSWDYRHAPLPQLILYF** RWGFSMLVRLVLNS*PQVIRLPRPPKVLGLQA* ATAPSQKMHDFLGSYAVCSHKVNHVSSNHDFGT P*IIKMRVEINRGKKIFFKPELCIC*VMGSIPLR SQTLQHQL*NTDNER***NIYDEKN*EVLN*DI SVKLIKFHCFHLKD*RY*YLDNWLIILKDYVVP AQLLIYCLL*NYHGYNSIGKDF*ITKISNVKRDK SQVNTILNTESENHYCIEVAFLIKSELQLVLEN YFL*LNSNQVLF*AVSLIEGP*K*SNDIVGDKG LVWSFSIKIEVAEVF*INNDLDCDLLDSVLSSVL YYFLKDNA*TLSISVLFMLIWQSFVN*IKTY*Y IGL*AKGKNEYYLSRYLKGKSLF*DSLSIENIR FLTCKYVCTPEELEEKQILEF*FNYIYSRLCL*C RILYILYKN*FFW*NEPFTVRFWTLSQRIFL*M LVSILVWSFVLFFRRNELKGTVA*SGFLS*CIG NK**TL

Sequence NM_014946

GGCCCGAGCCACCGACTGCAGGAGGAGAAGGGGTTGTGCTCCTGGCCGAGGAAGGAGAAAGGGGCGGGGCCGGCGGGCAGCGTGCGGCAGTGCGGAGCTC CTGAGACCGGCGGGCACACGGGGGTCTGTGGCCCCCGCCGTAGCAGTGGCTGCCGCCGTCGCTTGGTTCCCGTCGGTCTGCGGGAGGCGGGTTATGGCGG CGGCGGCAGTGAGAGCTGTGAATGAATTCTCCGGGTGGACGAGGGAAGAAGAAAGGCTCCGGCGGCGCCAGCAACCCGGTGCCTCCCAGGCCTCCGCCCC CTTGCCTGGCCCCCGCCCCTCCCGCCGCCGGGCCGGCCCCTCCGCCCGAGTCGCCGCATAAGCGGAACCTGTACTATTTCTCCTACCCGCTGTTTGTAGG CTTCGCGCTGCTGCGTTTGGTCGCCTTCCACCTGGGGCTCCTCTTCGTGTGGCTCTGCCAGCGCTTCTCCCGCGCCCTCATGGCAGCCAAGAGGAGCTCC GGGGCCGCGCCAGCACCTGCCTCGGCCTCGGCCCCGGCGCCGGTGCCGGGCGGCGAGGCCGAGCGCGTCCGAGTCTTCCACAAACAGGCCTTCGAGTACA TCTCCATTGCCCTGCGCATCGATGAGGATGAGAAAGCAGGACAGAAGGAGCAAGCTGTGGAATGGTATAAGAAAGGTATTGAAGAACTGGAAAAAGGAAT AGCTGTTATAGTTACAGGACAAGGTGAACAGTGTGAAAGAGCTAGACGCCTTCAAGCTAAAATGATGACTAATTTGGTTATGGCCAAGGACCGCTTACAA CTTCTAGAGAAGATGCAACCAGTTTTGCCATTTTCCAAGTCACAAACGGACGTCTATAATGACAGTACTAACTTGGCATGCCGCAATGGACATCTCCAGT CAGAAAGTGGAGCTGTTCCAAAAAGAAAAGACCCCTTAACACACACTAGTAATTCACTGCCTCGTTCAAAAACAGTTATGAAAACTGGATCTGCAGGCCT TTCAGGCCACCATAGAGCACCTAGTTACAGTGGTTTATCCATGGTTTCTGGAGTGAAACAGGGATCTGGTCCTGCTCCTACCACTCATAAGGGTACTCCG AAAACAAATAGGACAAATAAACCTTCTACCCCTACAACTGCTACTCGTAAGAAAAAAGACTTGAAGAATTTTAGGAATGTGGACAGCAACCTTGCTAACC TTATAATGAATGAAATTGTGGACAATGGAACAGCTGTTAAATTTGATGATATAGCTGGTCAAGACTTGGCAAAACAAGCATTGCAAGAAATTGTTATTCT TCCTTCTCTGAGGCCTGAGTTGTTCACAGGGCTTAGAGCTCCTGCCAGAGGGCTGTTACTCTTTGGTCCACCTGGGAATGGGAAGACAATGCTGGCTAAA GCAGTAGCTGCAGAATCGAATGCAACCTTCTTTAATATAAGTGCTGCAAGTTTAACTTCAAAATACGTGGGAGAAGGAGAGAAATTGGTGAGGGCTCTTT TTGCTGTGGCTCGAGAACTTCAACCTTCTATAATTTTTATAGATGAAGTTGATAGCCTTTTGTGTGAAAGAAGAGAAGGGGAGCACGATGCTAGTAGACG CCTAAAAACTGAATTTCTAATAGAATTTGATGGTGTACAGTCTGCTGGAGATGACAGAGTACTTGTAATGGGTGCAACTAATAGGCCACAAGAGCTTGAT GAGGCTGTTCTCAGGCGTTTCATCAAACGGGTATATGTGTCTTTACCAAATGAGGAGACAAGACTACTTTTGCTTAAAAATCTGTTATGTAAACAAGGAA GTCCATTGACCCAAAAAGAACTAGCACAACTTGCTAGAATGACTGATGGATACTCAGGAAGTGACCTAACAGCTTTGGCAAAAGATGCAGCACTGGGTCC TATCCGAGAACTAAAACCAGAACAGGTGAAGAATATGTCTGCCAGTGAGATGAGAAATATTCGATTATCTGACTTCACTGAATCCTTGAAAAAAATAAAA CGCAGCGTCAGCCCTCAAACTTTAGAAGCGTACATACGTTGGAACAAGGACTTTGGAGATACCACTGTTTAAGGAAATACCTTTGTAAACCTGCAGAACA TTTTACTTAAAAGAGGAAACACAAGATCTTCAATGAACGTCATCGGCTACAGAAACAGCCTAAGTTTACAGGACTTTTTAGAGTCTTACATATTTGTGCA CCAAACTTGAAGATGAACCAGAAAACAGACTTAAACAAAATATACAATGCAAATGTAATTTTTTGTTGTTTAAGGCCTTGCCTTGATGGTCACAGTTATC CCAATGGACACTAAGTTAGAGCACAACAAAACCTGATTCTGGTCTTCTTTACCAATATAATCATAATGTAAATAATAATTTGTATATTGTGTTGCAGATG AAAGTATTCCAGGAACAGTGAATGGTAGAAGACACAAGAACATTTGTTTGTTTGTCTTCTGATGTTTTTTCTTAAAATAGTAATTTCTCCTACTTTTCTT TTCTACTGTTGTCTTAACTACAGGTGATTGGAATGCCAAACACTCTTAAGTTTATTTTCTTTTTTCGTTTTATAAATTCAGTGTGCCAAATGAAACTTTT TTCCTAAGTAACTGTAATAGGAAAAAGTTTATTTTGAGAGTTTCTTCTTCATAAATCTACAGACATTAAACAATTGTTGTGTTCTTTTTACCTTTTATTT TTCTATTACCTTGCTACCAAACAGTTTAGATAGCAATATAATAGCAAAAAAGCAAATATGGTAAAATAGAGAAGGTTTGAAGGTTTGAGTTACTCTGTCA TATAACATGTAGATCAGTCTTCATGTGACCTGCAGTATTTTTTTTTCTAATGTATTTGTCAGAAATCTGTTGTAGACTGTTAACTTCTTCCTGATGGAAT TTATTTTCTGCAAGAATTATTCTGATATTTAAGAGAGCCAATTTTAACTGCTGTGAAAATGTTTCCAGTGCAAGAGAAGGGAAATACTAGGAACTAAGAC ATTTCTAATTTATTGCTTATTACTTTCTTAATTTTACAGGATAATTATAAGCAAGTGGAACTACCATCTTTTATTCTTAATAATTATTAATCCCTTCAAT GAAACTTTAAAAAAACTGAATTTTTATACATGGCATACATTTTTCTAGTTCCTTCTGCTTGCTTTATTAACTCAAAAGTTCTAGTTCTAGTCTGTTGATC TGCCTTTTGTTCTCCCAAAATGTACAGTAATTCCATTTGTTTGTATAAATATGCCTGGATTTTCATTATAAAAATGTCATTGTAGGGAGTAGAGACTCAT ATCATGGCCTTTTAAATATTGTAATAAAGGCAAATAGATATTTGCCCTTAGTTTACTGGTTAAAAGTTTGTTTACAGAACTTTTCTCTGGTGCTTAAATG ATGCTATGTAAAATGTCATGAGTGGAAAGAATATTTGTAGTAGTAACAAGAATTTTTCATTTAGGAAAGATTTCTTAGGTTTTGAAAGAATACATTAAAA TAAAAAACTTGCCCCTACTAGGTAAGAACTTTATAATGAAGACATACATTCTTCTTAATTTTACTCTTGCTCTTGTTAAAGATTTGTTTGAATATAGAAG ATGCATGATTTCTGGGTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCAATCTCGACTCACCACAACCTCC GCCTCCCAGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATGCGCCACTACCCCAGCTAATTTTGTATTTTTAGTAGAGA TGGGGTTTCTCCATGTTGGTCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATCCGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATAAGCCA CTGCGCCCAGCCAGAAGATGCATGATTTCTTAGGATCATATGCTGTTTGTAGCCATAAGGTAAATCATGTCTCTTCCAATCATGACTTTGGAACTCCCTG AATAATAAAAATGAGAGTTGAGATAAATAGGGGAAAAAAAATTTTTTTCAAGCCAGAGCTATGCATATGTTAGGTGATGGGTAGTATCCCTTTAAGGTCT CAAACATTACAACATCAATTATGAAATACTGATAACGAAAGGTAGTAATGAAATATATATGATGAAAAGAATTGAGAAGTTCTAAATTAAGACATTTCAG TTAAGCTCATAAAATTTCATTGTTTTCATTTAAAAGATTAACGTTATTGATACTTGGATAACTGGCTAATCATATTAAAGGACTATGTGGTTCCAGCTCA ACTTTTAATATATTGTCTCCTTTAAAACTATCATGGTTATAATTCTATTGGGAAAGACTTTTAGATAACAAAGATTTCAAATGTTAAAAGAGATAAAAGT CAGGTTAATACTATCTTAAACACTGAGTCAGAAAATCATTACTGTATAGAAGTTGCTTTCCTGATCAAGTCTGAACTTCAGCTAGTGCTAGAGAACTATT TTCTATGACTTAACTCTAACCAAGTTTTATTTTAAGCTGTTTCTTTGATAGAAGGGCCATGAAAATAGAGTAATGATATAGTAGGAGATAAGGGATTGGT TTGGTCTTTTTCAATAAAGATAGAAGTTGCTGAAGTTTTCTGAATTAATAATGACTTAGATTGTGACCTTTTAGATTCGGTGTTGAGCTCTGTGTTGTAT TACTTCCTAAAAGATAATGCTTAAACATTAAGCATTAGTGTGCTCTTCATGTTAATATGGCAGAGTTTTGTAAACTAAATTAAAACTTACTGATATATTG GACTTTGAGCCAAGGGAAAGAATGAGTACTATCTTTCCAGATATCTTAAGGGTAAAAGCTTATTCTAAGACAGTCTGTCCATTGAGAATATTAGATTTCT GACTTGCAAATATGTTTGTACTCCAGAAGAATTAGAGGAAAAGCAGATACTAGAATTCTAATTTAATTACATATACAGCCGTCTTTGTTTATAGTGTAGA ATTCTTTATATTTTGTACAAAAACTAATTCTTTTGGTAAAATGAACCATTTACAGTTCGGTTTTGGACTCTGAGTCAAAGGATTTTCCTTTAAATGCTTG TCTCAATTTTAGTCTGGTCTTTTGTACTTTTCTTCAGAAGAAATGAATTAAAGGGTACAGTTGCATAAAGTGGGTTTTTATCCTAATGTATTGGAAATAA ATGATAAACTTTA

refseq_SPAST.F1 (forward primer)
refseq_SPAST.R1 (reverse primer)
Single exon skipping

Frame 0

GPSHRLQEEKGLCSWPRKEKGAGPAGSVRQCGA PETGGHTGVCGPRRSSGCRRRLVPVGLREAGYGG GGSESCE*ILRVDEGRRKAPAAPATRCLPGLRP LAWPPPLPPPGRPLRPSRRISGTCTISPTRCL* ASRCCVWSPSTWGSSSCGSASASPAPSWQPRGAP GPRQHLPRPRPRRRCRAARPSASESSTNRPSST SPLPCASMRMRKQDRRSKLWNGIRKVLKNWKKE *LL*LQDKVNSVKELDAFKLK**LIWLWPRTAYN F*RRCNQFCHFPSHKRTSIMTVLTWHAAMDISS QKVELFQKEKTP*HTLVIHCLVQKQL*KLDLQA FQATIEHLVTVVYPWFLE*NRDLVLLLPLIRVLR KQIGQINLLPLQLLLVRKKT*RILGMWTATLLT L**MKLWTMEQLLNLMI*LVKTWQNKHCKKLLF FLL*GLSCSQGLELLPEGCYSLVHLGMGRQCWLK Q*LQNRMQPSLI*VLQV*LQNTWEKERNW*GLF LLWLENFNLL*FL*MKLIAFCVKEEKGSTMLVD A*KLNF**NLMVYSLLEMTEYL*WVQLIGHKSLM RLFSGVSSNGYMCLYQMRRQDYFCLKICYVNKE VH*PKKN*HNLLE*LMDTQEVT*QLWQKMQHWV LSEN*NQNR*RICLPVR*EIFDYLTSLNP*KK*N AASALKL*KRTYVGTRTLEIPLFKEIPL*TCRT FYLKEETQDLQ*TSSATETA*VYRTF*SLTYLC TKLEDEPENRLKQNIQCKCNFLLFKALP*WSQLS QWTLS*STTKPDSGLLYQYNHNVNNNLYIVLQM KVFQEQ*MVEDTRTFVCLSSDVFS*NSNFSYFS FLLLS*LQVIGMPNTLKFIFFFRFINSVCQMKLF S*VTVIGKSLF*EFLLHKSTDIKQLLCSFYLLF FYYLATKQFR*QYNSKKANMVK*RRFEGLSYSV I*HVDQSSCDLQYFFF*CICQKSVVDC*LLPDGI YFLQELF*YLREPILTAVKMFPVQEKGNTRN*D ISNLLLITFLILQDNYKQVELPSFILNNY*SLQ *NFKKTEFLYMAYIFLVPSACFINSKVLVLVC*S AFCSPKMYSNSICLYKYAWIFIIKMSL*GVETH IMAF*IL**RQIDICP*FTG*KFVYRTFLWCLN DAM*NVMSGKNICSSNKNFSFRKDFLGFERIH*N KKLAPTR*ELYNEDIHSS*FYSCSC*RFV*I*K MHDFWVFFFF*DRVSLLLPRLECNGAISTHHNL RLPGSSNSPASASRVAGITGMRHYPS*FCIFSRD GVSPCWSGWS*TPDLR*SACLGLPKCWDYRHKP LRPARRCMIS*DHMLFVAIR*IMSLPIMTLELP E**K*ELR*IGEKKFFSSQSYAYVR*WVVSL*GL KHYNINYEILITKGSNEIYMMKRIEKF*IKTFQ LSS*NFIVFI*KINVIDTWITG*SY*RTMWFQL NF*YIVSFKTIMVIILLGKTFR*QRFQMLKEIKV RLILS*TLSQKIITV*KLLS*SSLNFS*C*RTI FYDLTLTKFYFKLFL**KGHENRVMI**EIRDW FGLFQ*R*KLLKFSELIMT*IVTF*IRC*ALCCI TS*KIMLKH*ALVCSSC*YGRVL*TKLKLTDIL DFEPRERMSTIFPDILRVKAYSKTVCPLRILDF *LANMFVLQKN*RKSRY*NSNLITYTAVFVYSVE FFIFCTKTNSFGKMNHLQFGFGL*VKGFSFKCL SQF*SGLLYFSSEEMN*RVQLHKVGFYPNVLEI NDKL

Frame 1

ARATDCRRRRGCAPGRGRRKGRGRRAACGSAEL LRPAGTRGSVAPAVAVAAAVAWFPSVCGRRVMA AAAVRAVNEFSGWTREEERLRRRQQPGASQASAP LPGPRPSRRRAGPSARVAA*AEPVLFLLPAVCR LRAAAFGRLPPGAPLRVALPALLPRPHGSQEEL RGRASTCLGLGPGAGAGRRGRARPSLPQTGLRVH LHCPAHR*G*ESRTEGASCGMV*ERY*RTGKRN SCYSYRTR*TV*KS*TPSS*NDD*FGYGQGPLT TSREDATSFAIFQVTNGRL**QY*LGMPQWTSPV RKWSCSKKKRPLNTH**FTASFKNSYENWICRP FRPP*ST*LQWFIHGFWSETGIWSCSYHS*GYS ENK*DK*TFYPYNCYS*EKRLEEF*ECGQQPC*P YNE*NCGQWNSC*I**YSWSRLGKTSIARNCYS SFSEA*VVHRA*SSCQRAVTLWSTWEWEDNAG* SSSCRIECNLL*YKCCKFNFKIRGRRREIGEGSF CCGSRTSTFYNFYR*S**PFV*KKRRGARC**T PKN*ISNRI*WCTVCWR*QSTCNGCN**ATRA* *GCSQAFHQTGICVFTK*GDKTTFA*KSVM*TRK SIDPKRTSTTC*ND*WILRK*PNSFGKRCSTGS YPRTKTRTGEEYVCQ*DEKYSII*LH*ILEKNK TQRQPSNFRSVHTLEQGLWRYHCLRKYLCKPAEH FT*KRKHKIFNERHRLQKQPKFTGLFRVLHICA PNLKMNQKTDLNKIYNANVIFCCLRPCLDGHSY PNGH*VRAQQNLILVFFTNIIIM*IIICILCCR* KYSRNSEW*KTQEHLFVCLLMFFLKIVISPTFL FYCCLNYR*LECQTLLSLFSFFVL*IQCAK*NF FPK*L**EKVYFESFFFINLQTLNNCCVLFTFYF SITLLPNSLDSNIIAKKQIW*NREGLKV*VTLS YNM*ISLHVTCSIFFSNVFVRNLL*TVNFFLME FIFCKNYSDI*ESQF*LL*KCFQCKRREILGTKT FLIYCLLLS*FYRIIISKWNYHLLFLIIINPFN ETLKKLNFYTWHTFF*FLLLALLTQKF*F*SVD LPFVLPKCTVIPFVCINMPGFSL*KCHCRE*RLI SWPFKYCNKGK*IFALSLLVKSLFTELFSGA*M MLCKMS*VERIFVVVTRIFHLGKIS*VLKEYIK IKNLPLLGKNFIMKTYILLNFTLALVKDLFEYRR CMISGFFFFFETEFRSCCPGWSAMAQSRLTTTS ASQVQAILLPQPPE*LGLQACATTPANFVFLVE MGFLHVGQAGLELLTSGDPPASASQSAGITGISH CAQPEDA*FLRIICCL*P*GKSCLFQS*LWNSL NNKNES*DK*GKKNFFQARAMHMLGDG*YPFKV SNITTSIMKY**RKVVMKYI**KELRSSKLRHFS *AHKISLFSFKRLTLLILG*LANHIKGLCGSSS TFNILSPLKLSWL*FYWERLLDNKDFKC*KR*K SG*YYLKH*VRKSLLYRSCFPDQV*TSASARELF SMT*L*PSFILSCFFDRRAMKIE**YSRR*GIG LVFFNKDRSC*SFLN***LRL*PFRFGVELCVV LLPKR*CLNIKH*CALHVNMAEFCKLN*NLLIYW TLSQGKE*VLSFQIS*G*KLILRQSVH*EY*IS DLQICLYSRRIRGKADTRILI*LHIQPSLFIV* NSLYFVQKLILLVK*TIYSSVLDSESKDFPLNAC LNFSLVFCTFLQKK*IKGYSCIKWVFILMYWK* MINF

Frame 2

PEPPTAGGEGVVLLAEEGERGGAGGQRAAVRSS *DRRAHGGLWPPP*QWLPPSLGSRRSAGGGLWR RRQ*EL*MNSPGGRGKKKGSGGASNPVPPRPPP PCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVG FALLRLVAFHLGLLFVWLCQRFSRALMAAKRSS GAAPAPASASAPAPVPGGEAERVRVFHKQAFEY ISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGI AVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQ LLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQ SESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGL SGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTP KTNRTNKPSTPTTATRKKKDLKNFRNVDSNLAN LIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVIL PSLRPELFTGLRAPARGLLLFGPPGNGKTMLAK AVAAESNATFFNISAASLTSKYVGEGEKLVRAL FAVARELQPSIIFIDEVDSLLCERREGEHDASRR LKTEFLIEFDGVQSAGDDRVLVMGATNRPQELD EAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQG SPLTQKELAQLARMTDGYSGSDLTALAKDAALGP IRELKPEQVKNMSASEMRNIRLSDFTESLKKIK RSVSPQTLEAYIRWNKDFGDTTV*GNTFVNLQN ILLKRGNTRSSMNVIGYRNSLSLQDFLESYIFVH QT*R*TRKQT*TKYTMQM*FFVV*GLALMVTVI PMDTKLEHNKT*FWSSLPI*S*CK**FVYCVAD ESIPGTVNGRRHKNICLFVF*CFFLK**FLLLFF STVVLTTGDWNAKHS*VYFLFSFYKFSVPNETF FLSNCNRKKFILRVSSS*IYRH*TIVVFFLPFI FLLPCYQTV*IAI**QKSKYGKIEKV*RFELLCH ITCRSVFM*PAVFFFLMYLSEICCRLLTSS*WN LFSARIILIFKRANFNCCENVSSAREGKY*ELR HF*FIAYYFLNFTG*L*ASGTTIFYS**LLIPSM KL*KN*IFIHGIHFSSSFCLLY*LKSSSSSLLI CLLFSQNVQ*FHLFV*ICLDFHYKNVIVGSRDS YHGLLNIVIKANRYLPLVYWLKVCLQNFSLVLK* CYVKCHEWKEYL***QEFFI*ERFLRF*KNTLK *KTCPY*VRTL**RHTFFLILLLLLLKICLNIE DA*FLGFFFFLRQSFALVAQAGVQWRNLDSPQPP PPRFKQFSCLSLPSSWDYRHAPLPQLILYF**R WGFSMLVRLVLNS*PQVIRLPRPPKVLGLQA*A TAPSQKMHDFLGSYAVCSHKVNHVSSNHDFGTP* IIKMRVEINRGKKIFFKPELCIC*VMGSIPLRS QTLQHQL*NTDNER***NIYDEKN*EVLN*DIS VKLIKFHCFHLKD*RY*YLDNWLIILKDYVVPAQ LLIYCLL*NYHGYNSIGKDF*ITKISNVKRDKS QVNTILNTESENHYCIEVAFLIKSELQLVLENY FL*LNSNQVLF*AVSLIEGP*K*SNDIVGDKGLV WSFSIKIEVAEVF*INNDLDCDLLDSVLSSVLY YFLKDNA*TLSISVLFMLIWQSFVN*IKTY*YI GL*AKGKNEYYLSRYLKGKSLF*DSLSIENIRFL TCKYVCTPEELEEKQILEF*FNYIYSRLCL*CR ILYILYKN*FFW*NEPFTVRFWTLSQRIFL*ML VSILVWSFVLFFRRNELKGTVA*SGFLS*CIGNK **TL