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Sequence NM_173209
GTTTCTGGACCCGGCCACCCCCCGCGTCCGCACCGCCGCCCCCGAGGGACGAGTGACAGCGGCCGCCGCGCTCGGTCCAGTCTTCCCGGCTGGAAGGTATTGTTGCAGCATCTGGCAGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCTGGCTCAGGCAAGAGAAGGAGAAGGGGC
AACCTACCCAAGGAGTCTGTGCAGATTCTTCGGGATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTGTCCCAGC
AAACACACCTGTCTACGCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGCAGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCA
GTTCACAATTTCCCGCCGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAACTTCATGCCAGCTCTAGAGGAGACCCCA
TTTCATTCCTGTACAGCTGGGCCAAACCCAACCCTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCAGTGATCT
GCCATACCACTGTGACTGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCGGTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAA
CTTCACAGACACCTCTCTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGTCTTTTCAACACTCCTCCCCCTACTCCA
CCGGACCTCAACCAGGACTTCAGTGGATTTCAGCTTCTAGTGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACAGCTTAAC
CCATTTTCAAGCAAAACAGTTCTCAGAAATGTCATGATTGCCGGGGTGAAGGCAAGAGATGAATTGCATTATTTTATATATTTTTTATTAATATTTGCAC
ATGGGATTGCTAAAACAGCTTCCTGTTACTGAGATGTCTTCAATGGAATACAGTCATTCCAAGAACTATAAACTTAAAGCTACTGTAGAAACAAAGGGTT
TTCTTTTTTAAATGTTTCTTGGTAGATTATTCATAATGTGAGATGGTTCCCAATATCATGTGATTTTTTTTTTCCTCCCCTTCCCTTTTTTTGTTATTTT
TTCAGACTGTGCAATACTTAGAGAACCTATAGCATCTTCTCATTCCCATGTGGAACAGGATGCCCACATACTGTCTAATTAATAAATTTTCCATTTTTTT
TCAAACAAGTATGAATCTAGTTGGTTGATGCCTTTTTTTTCATGACATAATAAAGTATTTTCTTTAAAAA
- refseq_TGIF.F2 (forward primer)
- refseq_TGIF.R2 (reverse primer)
- Single exon skipping
Frame 0
VSGPGHPPRPHRRPRGTSDSGRRARSSLPGWKVLLQHLAVRLRMRTAWTFPWTFLHPLAQAREGEGA
TYPRSLCRFFGIGCMSTVTMPILQSKKKRCCPS
KHTCLRYRSVTGSSTPAAGSSLTC*ERMAKIQI
SSQFPAVGPRFLKRALWSP*WASKTSCQL*RRPH
FIPVQLGQTQP*GGHCLLSRHPRDQFWLVHQ*S
AIPL*LH*KMSLSLSASRSVWDKTQIYSR*RPK
TSQTPLSCTQRTLVNLDQVRIHRVVFSTLLPLLH
RTSTRTSVDFSF*WMLHSNGLQRWSFRQNLQLN
PFSSKTVLRNVMIAGVKARDELHYFIYFLLIFA
HGIAKTASCY*DVFNGIQSFQEL*T*SYCRNKGF
SFLNVSW*IIHNVRWFPISCDFFFPPLPFFCYF
FRLCNT*RTYSIFSFPCGTGCPHTV*LINFPFF
FKQV*I*LVDAFFFMT**SIFFK
Frame 1
FLDPATPRVRTAAPEGRVTAAAALGPVFPAGRYCCSIWQ*D*G*GQHGHSLGPFFIRWLRQEKEKG
QPTQGVCADSSGLAV*APLQCLSFRARKSVAVPA
NTPVYATGL*LVHQRPPQAPP*HAEKGWQRSKS
VHNFPPWGQDF*NELCGVRDGHQKLHASSRGDP
ISFLYSWAKPNPREATVS*AVIPGISFGSSISDL
PYHCDCIERCPFLSLPVGRCGTKHRYTADSGQK
LHRHLSHVPRGHL*IWTKYEYTEWSFQHSSPYS
TGPQPGLQWISASSGCCTQTGCRDGASGKTYSLT
HFQAKQFSEMS*LPG*RQEMNCIILYIFY*YLH
MGLLKQLPVTEMSSMEYSHSKNYKLKATVETKG
FLF*MFLGRLFIM*DGSQYHVIFFFLPFPFFVIF
SDCAILREPIASSHSHVEQDAHILSN**IFHFF
SNKYESSWLMPFFS*HNKVFSLK
Frame 2
FWTRPPPASAPPPPRDE*QRPPRSVQSSRLEGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRG
NLPKESVQILRDWLYEHRYNAYPSEQEKALLSQ
QTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQ
FTISRRGAKISETSSVESVMGIKNFMPALEETP
FHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVI
CHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKN
FTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTP
PDLNQDFSGFQLLVDVALKRAAEMELQAKLTA*
PIFKQNSSQKCHDCRGEGKR*IALFYIFFINICT
WDC*NSFLLLRCLQWNTVIPRTINLKLL*KQRV
FFFKCFLVDYS*CEMVPNIM*FFFSSPSLFLLF
FQTVQYLENL*HLLIPMWNRMPTYCLINKFSIFF
QTSMNLVG*CLFFHDIIKYFL*K
Sequence NM_173208
GTTTCTGGACCCGGCCACCCCCCGCGTCCGCACCGCCGCCCCCGAGGGACGAGTGACAGCGGCCGCCGCGCTCGGTCCAGTCTTCCCGGCTGGAAGGAGCGGGCGCCTGGGATCAGAGCGTCCTGTTTAGCAATAACGGCTGGAGCACGTCCTACAAGTTACGGGAGAGTCGGCTGTGAAGGAGACGTTCGCTTATCCCC
TGTGTCCCCGCTCCTGGCCCCTCCAGACCCCCGCCTTGCCTCGCGCTGGGAGGGGAGATCCAGAATGAAAGGCAAGAAAGGTATTGTTGCAGCATCTGGC
AGTGAGACTGAGGATGAGGACAGCATGGACATTCCCTTGGACCTTTCTTCATCCGCTGGCTCAGGCAAGAGAAGGAGAAGGGGCAACCTACCCAAGGAGT
CTGTGCAGATTCTTCGGGATTGGCTGTATGAGCACCGTTACAATGCCTATCCTTCAGAGCAAGAAAAAGCGTTGCTGTCCCAGCAAACACACCTGTCTAC
GCTACAGGTCTGTAACTGGTTCATCAACGCCCGCCGCAGGCTCCTCCCTGACATGCTGAGAAAGGATGGCAAAGATCCAAATCAGTTCACAATTTCCCGC
CGTGGGGCCAAGATTTCTGAAACGAGCTCTGTGGAGTCCGTGATGGGCATCAAAAACTTCATGCCAGCTCTAGAGGAGACCCCATTTCATTCCTGTACAG
CTGGGCCAAACCCAACCCTAGGGAGGCCACTGTCTCCTAAGCCGTCATCCCCGGGATCAGTTTTGGCTCGTCCATCAGTGATCTGCCATACCACTGTGAC
TGCATTGAAAGATGTCCCTTTCTCTCTCTGCCAGTCGGTCGGTGTGGGACAAAACACAGATATACAGCAGATAGCGGCCAAAAACTTCACAGACACCTCT
CTCATGTACCCAGAGGACACTTGTAAATCTGGACCAAGTACGAATACACAGAGTGGTCTTTTCAACACTCCTCCCCCTACTCCACCGGACCTCAACCAGG
ACTTCAGTGGATTTCAGCTTCTAGTGGATGTTGCACTCAAACGGGCTGCAGAGATGGAGCTTCAGGCAAAACTTACAGCTTAACCCATTTTCAAGCAAAA
CAGTTCTCAGAAATGTCATGATTGCCGGGGTGAAGGCAAGAGATGAATTGCATTATTTTATATATTTTTTATTAATATTTGCACATGGGATTGCTAAAAC
AGCTTCCTGTTACTGAGATGTCTTCAATGGAATACAGTCATTCCAAGAACTATAAACTTAAAGCTACTGTAGAAACAAAGGGTTTTCTTTTTTAAATGTT
TCTTGGTAGATTATTCATAATGTGAGATGGTTCCCAATATCATGTGATTTTTTTTTTCCTCCCCTTCCCTTTTTTTGTTATTTTTTCAGACTGTGCAATA
CTTAGAGAACCTATAGCATCTTCTCATTCCCATGTGGAACAGGATGCCCACATACTGTCTAATTAATAAATTTTCCATTTTTTTTCAAACAAGTATGAAT
CTAGTTGGTTGATGCCTTTTTTTTCATGACATAATAAAGTATTTTCTTTAAAAA
- refseq_TGIF.F2 (forward primer)
- refseq_TGIF.R2 (reverse primer)
- Single exon skipping
Frame 0
VSGPGHPPRPHRRPRGTSDSGRRARSSLPGWKERAPGIRASCLAITAGARPTSYGRVGCEGDVRLSP
VSPLLAPPDPRLASRWEGRSRMKGKKGIVAASG
SETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKE
SVQILRDWLYEHRYNAYPSEQEKALLSQQTHLST
LQVCNWFINARRRLLPDMLRKDGKDPNQFTISR
RGAKISETSSVESVMGIKNFMPALEETPFHSCT
AGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVT
ALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTS
LMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQ
DFSGFQLLVDVALKRAAEMELQAKLTA*PIFKQN
SSQKCHDCRGEGKR*IALFYIFFINICTWDC*N
SFLLLRCLQWNTVIPRTINLKLL*KQRVFFFKC
FLVDYS*CEMVPNIM*FFFSSPSLFLLFFQTVQY
LENL*HLLIPMWNRMPTYCLINKFSIFFQTSMN
LVG*CLFFHDIIKYFL*K
Frame 1
FLDPATPRVRTAAPEGRVTAAAALGPVFPAGRSGRLGSERPV*Q*RLEHVLQVTGESAVKETFAYP
LCPRSWPLQTPALPRAGRGDPE*KARKVLLQHLA
VRLRMRTAWTFPWTFLHPLAQAREGEGATYPRS
LCRFFGIGCMSTVTMPILQSKKKRCCPSKHTCL
RYRSVTGSSTPAAGSSLTC*ERMAKIQISSQFPA
VGPRFLKRALWSP*WASKTSCQL*RRPHFIPVQ
LGQTQP*GGHCLLSRHPRDQFWLVHQ*SAIPL*
LH*KMSLSLSASRSVWDKTQIYSR*RPKTSQTPL
SCTQRTLVNLDQVRIHRVVFSTLLPLLHRTSTR
TSVDFSF*WMLHSNGLQRWSFRQNLQLNPFSSK
TVLRNVMIAGVKARDELHYFIYFLLIFAHGIAKT
ASCY*DVFNGIQSFQEL*T*SYCRNKGFSFLNV
SW*IIHNVRWFPISCDFFFPPLPFFCYFFRLCN
T*RTYSIFSFPCGTGCPHTV*LINFPFFFKQV*I
*LVDAFFFMT**SIFFK
Frame 2
FWTRPPPASAPPPPRDE*QRPPRSVQSSRLEGAGAWDQSVLFSNNGWSTSYKLRESRL*RRRSLIP
CVPAPGPSRPPPCLALGGEIQNERQERYCCSIW
Q*D*G*GQHGHSLGPFFIRWLRQEKEKGQPTQGV
CADSSGLAV*APLQCLSFRARKSVAVPANTPVY
ATGL*LVHQRPPQAPP*HAEKGWQRSKSVHNFP
PWGQDF*NELCGVRDGHQKLHASSRGDPISFLYS
WAKPNPREATVS*AVIPGISFGSSISDLPYHCD
CIERCPFLSLPVGRCGTKHRYTADSGQKLHRHL
SHVPRGHL*IWTKYEYTEWSFQHSSPYSTGPQPG
LQWISASSGCCTQTGCRDGASGKTYSLTHFQAK
QFSEMS*LPG*RQEMNCIILYIFY*YLHMGLLK
QLPVTEMSSMEYSHSKNYKLKATVETKGFLF*MF
LGRLFIM*DGSQYHVIFFFLPFPFFVIFSDCAI
LREPIASSHSHVEQDAHILSN**IFHFFSNKYE
SSWLMPFFS*HNKVFSLK
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