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Sequences

Sequence NM_181762

ACACTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGACTTCGGAGAGGCAGCGCGGTTCCTCTGGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCC
AGCCTCTTCGGCCTCCTCGCCCGCCGCGGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCGCTCGCGACATGTCCACCCCGGCTCGGCGGCGC
CTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATTTTCG
GGCCTGAAGGGACCCCGTTTGAGGATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTATGATGTGTCTTCCAT
TCTAACATCCATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAA
AAGCGTGTTTCTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGACCCCGGGTACAGTTTAAAGAAGCTGGCCATAAGAAAAATATATATTGATGTGTTT
GTCACCTCCCTACTCCTGTCATTACATTTACTTTATTAAAAGCAAAATAACTGTTGTGCTGTTTCCATCTTCCTTGCCAAGTTTTCCTACCCCTTCTACC
CTCTCCTTAAACATCAGAAAACACCCTCTATGAAATCAAATGTACTGTACCTGGGTTACTTGCAAAAATTACTAATGCTTCAGTTTTTCTGTTGTATTTC
ATTTCCAGTTTTCAGGCAGTTATTTTTATTATTGTACTTTAAGCTTTTAAGATGAATTGTTATACAAGAGGTGCTTATGCTTAGCTTGATGACCAGGATG
TTATTTTTAACAAAATGATTGCTGAAGTGTTTCATCCTGGCTGGTCCTTCACTTGTGTTGGATTTAGAAGTGAATGTGTTTGGAATATGGCCTACAGAGA
ATAGAAACAAATCCATGTAAACAATTTTGAAGGAGGCATGGGAGCTAAAAATCCTGTGATACTAAGATCTCAGTCATATGAATTACAACGTAGTATTTAC
TGGCAAGAAGGAGAAAGTTGAAGGACTCAGCTAAAGGAGTACAGCAATTGTAGTAACTGACACATCCTCTCTTTGCAAGCTGCTGACTGGGCACACTCAT
GCCAAGTTTCAGAATTATTGGTCTTCTGGGTTTTTGCTTTTTAAAAGAGGTGTGGGAGCAGAGGAATGGAAACAATCGTGAGTTTTTGAGCTAGGGAAAG
TTGGAGCTCCTTTAATCTTTTTAAAGGATCAGTGCTGCCCTAAGTGAATAAACTCAATTGTCCATCTTTATTTTAGAGTTTTAATGAATTCAAGGAAGGG
AGCATAGCATATCTGTGGCAAACTATTTTCCACTCAAATCCTGAGTTATTGCTGCATGCTTTAATTTCTTCCCTTTCAGCATCTGAGAACCTTAAAGCCA
ATGTCTGCGATCTTTTTTTGGATATTTATACTTTTAGATATATAGTACCTTTAAGTAGCAGTATGGGACAAGGCTTGTAAATGTTTTGTCTAATGTTCTA
TTGTCACCTTTTATGCATTTATCACTTCCAAATCTAACTTTGCACAAGTAACCCATGTAAAAAAAAATGTACATTTTTCAAAAGTTGTAAATAAAAATAA
CCTTAAAA
  • refseq_UBE2A.F1 (forward primer)
  • refseq_UBE2A.R1 (reverse primer)
  • Single exon skipping

Frame 0

TLGWCLAGARPTLDFGEAARFLWVLPPPLLLLL
QPLRPPRPPREPETPVYAPPLTPLATCPPRLGGA
SCGTSRGCRRILQPESAGLRPRTT*WCGTRSFS
GLKGPRLRMSMQMVVYVWTYFRTVGVQPMMCLP
F*HPYSLCWMNPIPIVQQTARLLSCTRRTNGNMK
SVFLQ**NKAGVIVDPGYSLKKLAIRKIYIDVF
VTSLLLSLHLLY*KQNNCCAVSIFLAKFSYPFY
PLLKHQKTPSMKSNVLYLGYLQKLLMLQFFCCIS
FPVFRQLFLLLYFKLLR*IVIQEVLMLSLMTRM
LFLTK*LLKCFILAGPSLVLDLEVNVFGIWPTE
NRNKSM*TILKEAWELKIL*Y*DLSHMNYNVVFT
GKKEKVEGLS*RSTAIVVTDTSSLCKLLTGHTH
AKFQNYWSSGFLLFKRGVGAEEWKQS*VFELGK
VGAPLIFLKDQCCPK*INSIVHLYFRVLMNSRKG
A*HICGKLFSTQILSYCCML*FLPFQHLRTLKP
MSAIFFWIFILLDI*YL*VAVWDKACKCFV*CS
IVTFYAFITSKSNFAQVTHVKKNVHFSKVVNKNN
LK

Frame 1

HWGGA*PAPDRPSTSERQRGSSGCFRLPFSCFS
SLFGLLARRGNPRPQCMPHP*PRSRHVHPGSAA
PHAGLQEVAGGSSSRSQRGSVREQHNGVERGHFR
A*RDPV*GCLCRW*YMSGHTSEPLESNL*CVFH
SNIHTVSVG*TQSQ*SSKQPGCSAVPGEQTGI*
KACFCNSRTKLA*LLTPGTV*RSWP*EKYILMCL
SPPYSCHYIYFIKSKITVVLFPSSLPSFPTPST
LSLNIRKHPL*NQMYCTWVTCKNY*CFSFSVVF
HFQFSGSYFYYCTLSF*DELLYKRCLCLA**PGC
YF*QNDC*SVSSWLVLHLCWI*K*MCLEYGLQR
IETNPCKQF*RRHGS*KSCDTKISVI*ITT*YL
LARRRKLKDSAKGVQQL**LTHPLFASC*LGTLM
PSFRIIGLLGFCFLKEVWEQRNGNNREFLS*GK
LELL*SF*RISAALSE*TQLSIFILEF**IQGR
EHSISVANYFPLKS*VIAACFNFFPFSI*EP*SQ
CLRSFFGYLYF*IYSTFK*QYGTRLVNVLSNVL
LSPFMHLSLPNLTLHK*PM*KKMYIFQKL*IKI
TLK

Frame 2

TGVVLSRRQTDPRLRRGSAVPLGASASPSPASP
ASSASSPAAGTRDPSVCPTPDPARDMSTPARRR
LMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIF
GPEGTPFEDVYADGSICLDILQNRWSPTYDVSSI
LTSIQSLLDEPNPNSPANSQAAQLYQENKREYE
KRVSAIVEQSWRDC*PRVQFKEAGHKKNIY*CV
CHLPTPVITFTLLKAK*LLCCFHLPCQVFLPLLP
SP*TSENTLYEIKCTVPGLLAKITNASVFLLYF
ISSFQAVIFIIVL*AFKMNCYTRGAYA*LDDQD
VIFNKMIAEVFHPGWSFTCVGFRSECVWNMAYRE
*KQIHVNNFEGGMGAKNPVILRSQSYELQRSIY
WQEGES*RTQLKEYSNCSN*HILSLQAADWAHS
CQVSELLVFWVFAF*KRCGSRGMETIVSF*ARES
WSSFNLFKGSVLP*VNKLNCPSLF*SFNEFKEG
SIAYLWQTIFHSNPELLLHALISSLSASENLKA
NVCDLFLDIYTFRYIVPLSSSMGQGL*MFCLMFY
CHLLCIYHFQI*LCTSNPCKKKCTFFKSCK*K*
P*

Sequence NM_003336

ACACTGGGGTGGTGCTTAGCCGGCGCCAGACCGACCCTCGACTTCGGAGAGGCAGCGCGGTTCCTCTGGGTGCTTCCGCCTCCCCTTCTCCTGCTTCTCC
AGCCTCTTCGGCCTCCTCGCCCGCCGCGGGAACCCGAGACCCCAGTGTATGCCCCACCCCTGACCCCGCTCGCGACATGTCCACCCCGGCTCGGCGGCGC
CTCATGCGGGACTTCAAGAGGTTGCAGGAGGATCCTCCAGCCGGAGTCAGCGGGGCTCCGTCCGAGAACAACATAATGGTGTGGAACGCGGTCATTTTCG
GGCCTGAAGGGACCCCGTTTGAGGATGGAACATTTAAACTTACAATAGAATTCACTGAAGAATATCCAAATAAACCACCTACAGTTAGATTTGTCTCTAA
GATGTTCCATCCAAATGTCTATGCAGATGGTAGTATATGTCTGGACATACTTCAGAACCGTTGGAGTCCAACCTATGATGTGTCTTCCATTCTAACATCC
ATACAGTCTCTGTTGGATGAACCCAATCCCAATAGTCCAGCAAACAGCCAGGCTGCTCAGCTGTACCAGGAGAACAAACGGGAATATGAAAAGCGTGTTT
CTGCAATAGTAGAACAAAGCTGGCGTGATTGTTGACCCCGGGTACAGTTTAAAGAAGCTGGCCATAAGAAAAATATATATTGATGTGTTTGTCACCTCCC
TACTCCTGTCATTACATTTACTTTATTAAAAGCAAAATAACTGTTGTGCTGTTTCCATCTTCCTTGCCAAGTTTTCCTACCCCTTCTACCCTCTCCTTAA
ACATCAGAAAACACCCTCTATGAAATCAAATGTACTGTACCTGGGTTACTTGCAAAAATTACTAATGCTTCAGTTTTTCTGTTGTATTTCATTTCCAGTT
TTCAGGCAGTTATTTTTATTATTGTACTTTAAGCTTTTAAGATGAATTGTTATACAAGAGGTGCTTATGCTTAGCTTGATGACCAGGATGTTATTTTTAA
CAAAATGATTGCTGAAGTGTTTCATCCTGGCTGGTCCTTCACTTGTGTTGGATTTAGAAGTGAATGTGTTTGGAATATGGCCTACAGAGAATAGAAACAA
ATCCATGTAAACAATTTTGAAGGAGGCATGGGAGCTAAAAATCCTGTGATACTAAGATCTCAGTCATATGAATTACAACGTAGTATTTACTGGCAAGAAG
GAGAAAGTTGAAGGACTCAGCTAAAGGAGTACAGCAATTGTAGTAACTGACACATCCTCTCTTTGCAAGCTGCTGACTGGGCACACTCATGCCAAGTTTC
AGAATTATTGGTCTTCTGGGTTTTTGCTTTTTAAAAGAGGTGTGGGAGCAGAGGAATGGAAACAATCGTGAGTTTTTGAGCTAGGGAAAGTTGGAGCTCC
TTTAATCTTTTTAAAGGATCAGTGCTGCCCTAAGTGAATAAACTCAATTGTCCATCTTTATTTTAGAGTTTTAATGAATTCAAGGAAGGGAGCATAGCAT
ATCTGTGGCAAACTATTTTCCACTCAAATCCTGAGTTATTGCTGCATGCTTTAATTTCTTCCCTTTCAGCATCTGAGAACCTTAAAGCCAATGTCTGCGA
TCTTTTTTTGGATATTTATACTTTTAGATATATAGTACCTTTAAGTAGCAGTATGGGACAAGGCTTGTAAATGTTTTGTCTAATGTTCTATTGTCACCTT
TTATGCATTTATCACTTCCAAATCTAACTTTGCACAAGTAACCCATGTAAAAAAAAATGTACATTTTTCAAAAGTTGTAAATAAAAATAACCTTAAAA
  • refseq_UBE2A.F1 (forward primer)
  • refseq_UBE2A.R1 (reverse primer)
  • Single exon skipping

Frame 0

TLGWCLAGARPTLDFGEAARFLWVLPPPLLLLL
QPLRPPRPPREPETPVYAPPLTPLATCPPRLGGA
SCGTSRGCRRILQPESAGLRPRTT*WCGTRSFS
GLKGPRLRMEHLNLQ*NSLKNIQINHLQLDLSL
RCSIQMSMQMVVYVWTYFRTVGVQPMMCLPF*HP
YSLCWMNPIPIVQQTARLLSCTRRTNGNMKSVF
LQ**NKAGVIVDPGYSLKKLAIRKIYIDVFVTS
LLLSLHLLY*KQNNCCAVSIFLAKFSYPFYPLLK
HQKTPSMKSNVLYLGYLQKLLMLQFFCCISFPV
FRQLFLLLYFKLLR*IVIQEVLMLSLMTRMLFL
TK*LLKCFILAGPSLVLDLEVNVFGIWPTENRNK
SM*TILKEAWELKIL*Y*DLSHMNYNVVFTGKK
EKVEGLS*RSTAIVVTDTSSLCKLLTGHTHAKF
QNYWSSGFLLFKRGVGAEEWKQS*VFELGKVGAP
LIFLKDQCCPK*INSIVHLYFRVLMNSRKGA*H
ICGKLFSTQILSYCCML*FLPFQHLRTLKPMSA
IFFWIFILLDI*YL*VAVWDKACKCFV*CSIVTF
YAFITSKSNFAQVTHVKKNVHFSKVVNKNNLK

Frame 1

HWGGA*PAPDRPSTSERQRGSSGCFRLPFSCFS
SLFGLLARRGNPRPQCMPHP*PRSRHVHPGSAA
PHAGLQEVAGGSSSRSQRGSVREQHNGVERGHFR
A*RDPV*GWNI*TYNRIH*RISK*TTYS*ICL*
DVPSKCLCRW*YMSGHTSEPLESNL*CVFHSNI
HTVSVG*TQSQ*SSKQPGCSAVPGEQTGI*KACF
CNSRTKLA*LLTPGTV*RSWP*EKYILMCLSPP
YSCHYIYFIKSKITVVLFPSSLPSFPTPSTLSL
NIRKHPL*NQMYCTWVTCKNY*CFSFSVVFHFQF
SGSYFYYCTLSF*DELLYKRCLCLA**PGCYF*
QNDC*SVSSWLVLHLCWI*K*MCLEYGLQRIET
NPCKQF*RRHGS*KSCDTKISVI*ITT*YLLARR
RKLKDSAKGVQQL**LTHPLFASC*LGTLMPSF
RIIGLLGFCFLKEVWEQRNGNNREFLS*GKLEL
L*SF*RISAALSE*TQLSIFILEF**IQGREHSI
SVANYFPLKS*VIAACFNFFPFSI*EP*SQCLR
SFFGYLYF*IYSTFK*QYGTRLVNVLSNVLLSP
FMHLSLPNLTLHK*PM*KKMYIFQKL*IKITLK

Frame 2

TGVVLSRRQTDPRLRRGSAVPLGASASPSPASP
ASSASSPAAGTRDPSVCPTPDPARDMSTPARRR
LMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIF
GPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSK
MFHPNVYADGSICLDILQNRWSPTYDVSSILTS
IQSLLDEPNPNSPANSQAAQLYQENKREYEKRV
SAIVEQSWRDC*PRVQFKEAGHKKNIY*CVCHLP
TPVITFTLLKAK*LLCCFHLPCQVFLPLLPSP*
TSENTLYEIKCTVPGLLAKITNASVFLLYFISS
FQAVIFIIVL*AFKMNCYTRGAYA*LDDQDVIFN
KMIAEVFHPGWSFTCVGFRSECVWNMAYRE*KQ
IHVNNFEGGMGAKNPVILRSQSYELQRSIYWQE
GES*RTQLKEYSNCSN*HILSLQAADWAHSCQVS
ELLVFWVFAF*KRCGSRGMETIVSF*ARESWSS
FNLFKGSVLP*VNKLNCPSLF*SFNEFKEGSIA
YLWQTIFHSNPELLLHALISSLSASENLKANVCD
LFLDIYTFRYIVPLSSSMGQGL*MFCLMFYCHL
LCIYHFQI*LCTSNPCKKKCTFFKSCK*K*P*

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