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Sequences

Sequence NM_001025203

GTCCGGGTGACGTCTCCCGAGGGCGTCGGCAGGGTCGGCGGCGTCGGCAGCAGTGTCGACGGCAGCGGCGGCGGCGGGTGGGAAATGGCGGAGTATCTGG CCTCCATCTTCGGCACCGAGAAAGACAAAGTCAACTGTTCATTTTATTTCAAAATTGGAGCATGTCGTCATGGAGACAGGTGCTCTCGGTTGCACAATAA ACCGACGTTTAGCCAGACCATCTTGATTCAAAACATCTATCGTAATCCCCAAAACAGTGCACAGACGGCTGACGGCTCACACTGTGCCGTGAGCGATGTG GAGATGCAGGAACACTATGATGAGTTTTTTGAGGAGGTTTTTACAGAAATGGAGGAGAAGTATGGGGAAGTAGAGGAGATGAACGTCTGTGACAACCTGG GAGACCACCTGGTGGGGAACGTGTACGTCAAGTTTCGCCGTGAGGAAGATGCGGAAAAGGCTGTGATTGACTTGAATAACCGTTGGTTTAATGGACAGCC GATCCACGCCGAGCTGTCACCCGTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGAGAATGCACACGAGGCGGCTTCTGCAACTTCATG CATTTGAAGCCCATTTCCAGAGAGCTGCGGCGGGAGCTGTATGGCCGCCGTCGCAAGAAGCATAGATCAAGATCCCGATCCCGGGAGCGTCGTTCTCGGT CTAGAGACCGTGGTCGTGGCGGTGGCGGTGGCGGTGGTGGAGGTGGCGGCGGACGGGAGCGTGACAGGAGGCGGTCGAGAGATCGTGAAAGATCTGGGCG ATTCTGAGCCATGCCATTTTTACCTTATGTCTGCTAGAAAGTGTTGTAGTTGATTGACCAAACCAGTTCATAAGGGGAATTTTTTTTAAAAAACAACAAA AAAAAAAACATACAAAGATGGGTTTCTGAATAAAATTTGTAGTGATAACAGTA

refseq_U2AF1.F4 (forward primer)
refseq_U2AF1.R4 (reverse primer)
Single exon skipping

Frame 0

VRVTSPEGVGRVGGVGSSVDGSGGGGWEMAEYL ASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNK PTFSQTILIQNIYRNPQNSAQTADGSHCAVSDV EMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNL GDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQP IHAELSPVTDFREACCRQYEMGECTRGGFCNFM HLKPISRELRRELYGRRRKKHRSRSRSRERRSR SRDRGRGGGGGGGGGGGGRERDRRRSRDRERSGR F*AMPFLPYVC*KVL*LIDQTSS*GEFFLKNNK KKNIQRWVSE*NL***Q

Frame 1

SG*RLPRASAGSAASAAVSTAAAAAGGKWRSIW PPSSAPRKTKSTVHFISKLEHVVMETGALGCTI NRRLARPS*FKTSIVIPKTVHRRLTAHTVP*AMW RCRNTMMSFLRRFLQKWRRSMGK*RR*TSVTTW ETTWWGTCTSSFAVRKMRKRL*LT*ITVGLMDS RSTPSCHP*RTSEKPAAVSMRWENAHEAASATSC I*SPFPESCGGSCMAAVARSIDQDPDPGSVVLG LETVVVAVAVAVVEVAADGSVTGGGREIVKDLG DSEPCHFYLMSARKCCS*LTKPVHKGNFF*KTTK KKTYKDGFLNKICSDNS

Frame 2

PGDVSRGRRQGRRRRQQCRRQRRRRVGNGGVSG LHLRHRERQSQLFILFQNWSMSSWRQVLSVAQ* TDV*PDHLDSKHLS*SPKQCTDG*RLTLCRERC GDAGTL**VF*GGFYRNGGEVWGSRGDERL*QPG RPPGGERVRQVSP*GRCGKGCD*LE*PLV*WTA DPRRAVTRDGLQRSLLPSV*DGRMHTRRLLQLH AFEAHFQRAAAGAVWPPSQEA*IKIPIPGASFSV *RPWSWRWRWRWWRWRRTGA*QEAVERS*KIWA ILSHAIFTLCLLESVVVD*PNQFIRGIFFKKQQ KKKHTKMGF*IKFVVITV

Sequence NM_001025204

GTCCGGGTGACGTCTCCCGAGGGCGTCGGCAGGGTCGGCGGCGTCGGCAGCAGTGTCGACGGCAGCGGCGGCGGCGGGTGGGAAATGGCGGAGTATCTGG CCTCCATCTTCGGCACCGAGAAAGACAAAGTCAACTGTTCATTTTATTTCAAAATTGGAGCATGTCGTCATGGAGACAGGTGCTCTCGGTTGCACAATAA ACCGACGTTTAGCCAGACCATCTTGATTCAAAACATCTATCGTAATCCCCAAAACAGTGCACAGACGGCTGACGGCTCACACTACCATTGCCCTCTTGAA CATTTACCGTAACCCTCAAAACTCTTCCCAGTCTGCTGACGGTTTGCGCTGTGCCGTGAGCGATGTGGAGATGCAGGAACACTATGATGAGTTTTTTGAG GAGGTTTTTACAGAAATGGAGGAGAAGTATGGGGAAGTAGAGGAGATGAACGTCTGTGACAACCTGGGAGACCACCTGGTGGGGAACGTGTACGTCAAGT TTCGCCGTGAGGAAGATGCGGAAAAGGCTGTGATTGACTTGAATAACCGTTGGTTTAATGGACAGCCGATCCACGCCGAGCTGTCACCCGTGACGGACTT CAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGAGAATGCACACGAGGCGGCTTCTGCAACTTCATGCATTTGAAGCCCATTTCCAGAGAGCTGCGGCGG GAGCTGTATGGCCGCCGTCGCAAGAAGCATAGATCAAGATCCCGATCCCGGGAGCGTCGTTCTCGGTCTAGAGACCGTGGTCGTGGCGGTGGCGGTGGCG GTGGTGGAGGTGGCGGCGGACGGGAGCGTGACAGGAGGCGGTCGAGAGATCGTGAAAGATCTGGGCGATTCTGAGCCATGCCATTTTTACCTTATGTCTG CTAGAAAGTGTTGTAGTTGATTGACCAAACCAGTTCATAAGGGGAATTTTTTTTAAAAAACAACAAAAAAAAAAACATACAAAGATGGGTTTCTGAATAA AATTTGTAGTGATAACAGTA

refseq_U2AF1.F4 (forward primer)
refseq_U2AF1.R4 (reverse primer)
Single exon skipping

Frame 0

VRVTSPEGVGRVGGVGSSVDGSGGGGWEMAEYL ASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNK PTFSQTILIQNIYRNPQNSAQTADGSHYHCPLE HLP*PSKLFPVC*RFALCRERCGDAGTL**VF* GGFYRNGGEVWGSRGDERL*QPGRPPGGERVRQV SP*GRCGKGCD*LE*PLV*WTADPRRAVTRDGL QRSLLPSV*DGRMHTRRLLQLHAFEAHFQRAAA GAVWPPSQEA*IKIPIPGASFSV*RPWSWRWRWR WWRWRRTGA*QEAVERS*KIWAILSHAIFTLCL LESVVVD*PNQFIRGIFFKKQQKKKHTKMGF*I KFVVITV

Frame 1

SG*RLPRASAGSAASAAVSTAAAAAGGKWRSIW PPSSAPRKTKSTVHFISKLEHVVMETGALGCTI NRRLARPS*FKTSIVIPKTVHRRLTAHTTIALLN IYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFE EVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVK FRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDF REACCRQYEMGECTRGGFCNFMHLKPISRELRR ELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGG GGGGGGGRERDRRRSRDRERSGRF*AMPFLPYVC *KVL*LIDQTSS*GEFFLKNNKKKNIQRWVSE* NL***Q

Frame 2

PGDVSRGRRQGRRRRQQCRRQRRRRVGNGGVSG LHLRHRERQSQLFILFQNWSMSSWRQVLSVAQ* TDV*PDHLDSKHLS*SPKQCTDG*RLTLPLPS* TFTVTLKTLPSLLTVCAVP*AMWRCRNTMMSFLR RFLQKWRRSMGK*RR*TSVTTWETTWWGTCTSS FAVRKMRKRL*LT*ITVGLMDSRSTPSCHP*RT SEKPAAVSMRWENAHEAASATSCI*SPFPESCGG SCMAAVARSIDQDPDPGSVVLGLETVVVAVAVA VVEVAADGSVTGGGREIVKDLGDSEPCHFYLMS ARKCCS*LTKPVHKGNFF*KTTKKKTYKDGFLNK ICSDNS