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Sequences

Sequence NM_001005409

ACCTTCGCGGCCGCAACTCGCTCGGCCGCCGCCATCTTGCGAGCTCGTCGTACTGACCGAGCGGGGAGGCTGTCTTGAGGCGGCACCGCTCACCGACACC
GAGGCGGACTGGCAGCCCTGAGCGTCGCAGTCATGCCGGCCGGACCCGTGCAGGCGGTGCCCCCGCCGCCGCCCGTGCCCACGGAGCCCAAACAGCCCAC
AGAAGAAGAAGCATCTTCAAAGGAGGATTCTGCACCTTCTAAGCCAGTTGTGGGGATTATTTACCCTCCTCCAGAGGTCAGAAATATTGTTGACAAGACT
GCCAGCTTTGTGGCCAGAAACGGGCCTGAATTTGAAGCTAGGATCCGACAGAACGAGATCAACAACCCCAAGTTCAACTTTCTGAACCCCAATGACCCTT
ACCATGCCTACTACCGCCACAAGGTCAGCGAGTTCAAGGAAGGGAAGGCTCAGTTTGTGGCCAGGAATGGGCGCCAGTTTCTGACCCAGCTGATGCAGAA
AGAGCAGCGCAACTACCAGTTTGACTTTCTCCGCCCACAGCACAGCCTCTTCAACTACTTCACGAAGCTAGTGGAACAGTACACCAAGATCTTGATTCCA
CCCAAAGGTTTATTTTCAAAGCTCAAGAAAGAGGCTGAAAACCCCCGAGAAGTTTTGGATCAGGTGTGTTACCGAGTGGAATGGGCCAAATTCCAGGAAC
GTGAGAGGAAGAAGGAAGAAGAGGAGAAGGAGAAGGAGCGGGTGGCCTATGCTCAGATCGACTGGCATGATTTTGTGGTGGTGGAAACAGTGGACTTCCA
ACCCAATGAGCAAGGGAACTTCCCTCCCCCCACCACGCCAGAGGAGCTGGGGGCCCGAATCCTCATTCAGGAGCGCTATGAAAAGTTTGGGGAGAGTGAG
GAAGTTGAGATGGAGGTCGAGTCTGATGAGGAGGATGACAAACAGGAGAAGGCGGAGGAGCCTCCTTCCCAGCTGGACCAGGACACCCAAGTACAAGATA
TGGATGAGGGTTCAGATGATGAAGAAGAAGGGCAGAAAGTGCCCCCACCCCCAGAGACACCCATGCCTCCACCTCTGCCCCCAACTCCAGACCAAGTCAT
TGTCCGCAAGGATTATGATCCCAAAGCCTCCAAGCCCTTGCCTCCAGCCCCTGCTCCAGATGAGTATCTTGTGTCCCCCATTACTGGGGAGAAGATCCCC
GCCAGCAAAATGCAGGAACACATGCGCATTGGACTTCTTGACCCTCGCTGGCTGGAGCAGCGGGATCGCTCCATCCGTGAGAAGCAGAGCGATGATGAGG
TGTACGCACCAGGTCTGGATATTGAGAGCAGCTTGAAGCAGTTGGCTGAGCGGCGTACTGACATCTTCGGTGTAGAGGAAACAGCCATTGGTAAGAAGAT
CGGTGAGGAGGAGATCCAGAAGCCAGAGGAAAAGGTGACCTGGGATGGCCACTCAGGCAGCATGGCCCGGACCCAGCAGGCTGCCCAGGCCAACATCACC
CTCCAGGAGCAGATTGAGGCCATTCACAAGGCCAAAGGCCTGGTGCCAGAGGATGACACTAAAGAGAAGATTGGCCCCAGCAAGCCCAATGAAATCCCTC
AACAGCCACCGCCACCATCTTCAGCCACCAACATCCCCAGCTCGGCTCCACCCATCACTTCAGTGCCCCGACCACCCACAATGCCACCTCCAGTTCGTAC
TACAGTTGTCTCCGCAGTACCCGTCATGCCCCGGCCCCCAATGGCATCTGTGGTCCGGCTGCCCCCAGGCTCAGTGATCGCCCCCATGCCGCCCATCATC
CACGCGCCCAGAATCAACGTGGTGCCCATGCCTCCCTCGGCCCCTCCTATTATGGCCCCCCGCCCACCCCCCATGATTGTGCCAACAGCCTTTGTGCCTG
CTCCACCTGTGGCACCTGTCCCAGCTCCAGCCCCAATGCCCCCTGTGCATCCCCCACCTCCCATGGAAGATGAGCCCACCTCCAAAAAACTGAAGACAGA
GGACAGCCTCATGCCAGAGGAGGAGTTCCTGCGCAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATAAGACGGAATGGAAA
CTGAATGGGCAGGTGCTGGTCTTCACCCTCCCACTCACGGACCAGGTCTCTGTCATTAAGGTGAAGATTCATGAAGCCACAGGCATGCCTGCAGGGAAAC
AGAAGCTACAGTATGAGGGTATCTTCATCAAAGATTCCAACTCACTGGCTTACTACAACATGGCCAATGGCGCAGTCATCCACCTGGCCCTCAAGGAGAG
AGGCGGGAGGAAGAAGTAGACAAGAGGAACCTGCTGTCAAGTCCCTGCCATTTTGCCTCTCCTGTCTCCCACCCCCTGCCCCAGACCCAGGAGCCCCCCT
GAGGCTTTGCCTTGCCTGCATATTTGTTTCGCTCTTACTCAGTTTGGGAATTCAAATTGTCCTGCAGAGGTTCATTCCCCTGACCCTTTCCCCACATTGG
TAAGAGTAGCTGGGTTTTCTAAGCCACTCTCTGGAATCTCTTTGTGTTAGGGTCTCGATTTGAGGACATTCATTTCTTCAGCAGCCCATTAGCAACTGAG
AGCCCAGGGATGTCCTACAGGATAGTTTCATAGTGACAGGTGGCACTTGGCTAATAGAATATGGCTGATATTGTCATTAATCATTTTGTACCTTGACATG
GGTTGTCTAATAAAACTCGGACCCTTCTTGTGAAATCAGTTAAATAAGACTTGTCTCGGTCACCTGTGCCCTGTCCAGACTCGAGGCAGTGGTAACACTG
CACAGTGCTATGTGGCTTCTCTTTGAGGATTTTTGGGTTTTGTAACTAAATTCTTGCTGCCCTCATACTTTTTATGTATTAGAATCATATTCGTATTGCC
CTTTTAAAACATTGGGATCCTCCAAAGGCCTGCCCCATGTATTTAACAGTAATACAGGAAGCATGGCAGGCACCATGCAAACCAAGGATGGATGGTGCAG
TCCCTGTGTCAGTGGGCGGTGGTTTCCTGCTGGCCTGGAATCACTCATCACCTGATTGATTGGCTCTGTGGTCCTGGGCAGGTGCCTCATAGGTGTGTGG
ATATGATGACGTTTCTTTAAAATGTATGTATTTAACAAATACTTAATTGTATTAAGGTCATGTACCAAGGATTTGATAAAGTTTAAATAATTTACTCTCT
ACTTTTATCCATTTTATCCATTTTAACTCATGTAATCCTCATGTGAGTATTCCTGTTTAACACTTGAGTAAACTGAGGCACAGAGAACATAAGTTGCATG
CCATAGTCACACACTGTGAAAGTGAAAAGAGAATGTGTGCAAAACACGTCACAGTCCTGGTTTCTGAGTAAAGGCAGGCTGTTATCTTTAGAATCAAGCT
ATCACAGGGAGATAGGCAATGCTGTGGGTGTTGGAGGAAGGTGAGAGCCTGTTGCTAACAATTTCCTGGTTTTAAAGCTAAGGCTGATTTTATTGGGAAG
ATCTCACATGTGTGTGGCCCCTGAGAGTTCCCAGTGCCTTTTATTTGCAGTCCTTCCATTTGGACCTCCTAGCTGCCCCATCAGGTCATCTCCAGGGCTC
AGAGGGGTGAGACCATTTCCCAAGGTCACAGAACCAGCTCTCTAGTCACCACCCTGCCTCTCCCTCTCACCCAGAGTCAGTACCAGTTTTATGGCTTTAT
TACAAACTGCTGGGTCCCTCCCATTTTCAACTTGATTGATGGGATGTCATCCCTTATCCTGTCTGACATTTGCCTCTGGCCTGGTTGCTAGAAGTTTGCC
CCAGGGGCAAGAGTTGAAATTTGGCTTCCTGAGGTGGGCTTTGTGGTTTGCGTCCCTAAAGTGAGCCCACTACTGGTTGCTTGTCCATGGCCAACACCAG
AAATCCCCTGAGCACTACCTGGGTCTCATTCCAAGAAGGAAGAGGGTCAGGAGACCTGGGGAGTCTCATATTCCAAGTTCTTCTTTCTTTCTGGGAGCAG
TGGGCAGTTCATGGTGTTAGGGCACTCACCCCCACAGACTGGCAAACCCTGCAGGACTTCCGTGGCTGAGGCTGTGACCGGAGGCCAGGAATGCCGTTGG
GTGGATTGTGAGTGAATGGGCCCTTTGAGCTGCCCTCTAGAGAGCAAATCCAGTTTCCTGGAGCTCCTGAATGAATATCTGTACTGGCTCGCTCAGATGC
AGAAGCTCCATTGACCATGAGGCCTTGTGAACATCAGTGGCCACAGGCCCAGTGTGCTGCTTGGCACTGCACTAGTTTAGGACCTGCAGCATGTAGGTAG
CGTCCTAGTGTTTATAATACAAAGCTGCTCTGCACAGCTTTTCTGATTCTTCTTGCAATCTCCTGAGGATTATCTGCCCCATTTTTAAAACGAGGTGGAA
TACCCAAGGTCATGTAGCCAGTGAGTGCTCTGGAAAGCCAAAGCAGCTCATCCCTTCCTGGGGACCACACTGCTCTGCTCCACCAGACCACACTATGAAA
TAGGAATAAGTGCTCCTGTTGCAGGACTGCTGGGAAAACAGGTGGTGTGGGACTTAAGTCACCATAATTTTGAAGACTTGCATGCAGAGGGCTCCAGGAA
TTGTAGACATTAAGGAATTTCACTTTCAGTTCTACCCACTACTTAAGTACTTGTCATGTACTCTTAGAGGAGGCCAGTAATGATCAGAACCATTTTACTT
TAAAATTAATAATATTGTATTAGAGAATATATTAAATGGTTATATTGGGTTATGTTAGGATATATACTTGAATGGAAATACATGTACTATTAGCAATCAT
ATTTCATTTATCCCTGTAATTAGACAAGAAAGCATAATATAGCTCTACTCATGGGTACACATACCAGTGTATAAGATTTTTAGAAGTTTACTTTTTAAAA
ATAAAAGCAAAATGTAAGATCTTAA
  • refseq_SF3A1.F1 (forward primer)
  • refseq_SF3A1.R1 (reverse primer)
  • Alternative donor and acceptor

Frame 0

TFAAATRSAAAILRARRTDRAGRLS*GGTAHRH
RGGLAALSVAVMPAGPVQAVPPPPPVPTEPKQPT
EEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKT
ASFVARNGPEFEARIRQNEINNPKFNFLNPNDP
YHAYYRHKVSEFKEGKAQFVARNGRQFLTQLMQK
EQRNYQFDFLRPQHSLFNYFTKLVEQYTKILIP
PKGLFSKLKKEAENPREVLDQVCYRVEWAKFQE
RERKKEEEEKEKERVAYAQIDWHDFVVVETVDFQ
PNEQGNFPPPTTPEELGARILIQERYEKFGESE
EVEMEVESDEEDDKQEKAEEPPSQLDQDTQVQD
MDEGSDDEEEGQKVPPPPETPMPPPLPPTPDQVI
VRKDYDPKASKPLPPAPAPDEYLVSPITGEKIP
ASKMQEHMRIGLLDPRWLEQRDRSIREKQSDDE
VYAPGLDIESSLKQLAERRTDIFGVEETAIGKKI
GEEEIQKPEEKVTWDGHSGSMARTQQAAQANIT
LQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIP
QQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRT
TVVSAVPVMPRPPMASVVRLPPGSVIAPMPPII
HAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVP
APPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTE
DSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWK
LNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGK
QKLQYEGIFIKDSNSLAYYNMANGAVIHLALKER
GGRKK*TRGTCCQVPAILPLLSPTPCPRPRSPP
EALPCLHICFALTQFGNSNCPAEVHSPDPFPTL
VRVAGFSKPLSGISLC*GLDLRTFISSAAH*QLR
AQGCPTG*FHSDRWHLANRIWLILSLIILYLDM
GCLIKLGPFL*NQLNKTCLGHLCPVQTRGSGNT
AQCYVASL*GFLGFVTKFLLPSYFLCIRIIFVLP
F*NIGILQRPAPCI*Q*YRKHGRHHANQGWMVQ
SLCQWAVVSCWPGITHHLIDWLCGPGQVPHRCV
DMMTFL*NVCI*QILNCIKVMYQGFDKV*IIYSL
LLSILSILTHVILM*VFLFNT*VN*GTENISCM
P*SHTVKVKRECVQNTSQSWFLSKGRLLSLESS
YHREIGNAVGVGGR*EPVANNFLVLKLRLILLGR
SHMCVAPESSQCLLFAVLPFGPPSCPIRSSPGL
RGVRPFPKVTEPAL*SPPCLSLSPRVSTSFMAL
LQTAGSLPFST*LMGCHPLSCLTFASGLVARSLP
QGQELKFGFLRWALWFASLK*AHYWLLVHGQHQ
KSPEHYLGLIPRRKRVRRPGESHIPSSSFFLGA
VGSSWC*GTHPHRLANPAGLPWLRL*PEARNAVG
WIVSEWAL*AAL*RANPVSWSS*MNICTGSLRC
RSSIDHEAL*TSVATGPVCCLALH*FRTCSM*V
AS*CL*YKAALHSFSDSSCNLLRIICPIFKTRWN
TQGHVASECSGKPKQLIPSWGPHCSAPPDHTMK
*E*VLLLQDCWENRWCGT*VTIILKTCMQRAPG
IVDIKEFHFQFYPLLKYLSCTLRGGQ**SEPFYF
KINNIVLENILNGYIGLC*DIYLNGNTCTISNH
ISFIPVIRQESII*LYSWVHIPVYKIFRSLLFK
NKSKM*DL

Frame 1

PSRPQLARPPPSCELVVLTERGGCLEAAPLTDT
EADWQP*ASQSCRPDPCRRCPRRRPCPRSPNSP
QKKKHLQRRILHLLSQLWGLFTLLQRSEILLTRL
PALWPETGLNLKLGSDRTRSTTPSSTF*TPMTL
TMPTTATRSASSRKGRLSLWPGMGASF*PS*CR
KSSATTSLTFSAHSTASSTTSRS*WNSTPRS*FH
PKVYFQSSRKRLKTPEKFWIRCVTEWNGPNSRN
VRGRRKKRRRRRSGWPMLRSTGMILWWWKQWTS
NPMSKGTSLPPPRQRSWGPESSFRSAMKSLGRVR
KLRWRSSLMRRMTNRRRRRSLLPSWTRTPKYKI
WMRVQMMKKKGRKCPHPQRHPCLHLCPQLQTKS
LSARIMIPKPPSPCLQPLLQMSILCPPLLGRRSP
PAKCRNTCALDFLTLAGWSSGIAPSVRSRAMMR
CTHQVWILRAA*SSWLSGVLTSSV*RKQPLVRR
SVRRRSRSQRKR*PGMATQAAWPGPSRLPRPTSP
SRSRLRPFTRPKAWCQRMTLKRRLAPASPMKSL
NSHRHHLQPPTSPARLHPSLQCPDHPQCHLQFV
LQLSPQYPSCPGPQWHLWSGCPQAQ*SPPCRPSS
TRPESTWCPCLPRPLLLWPPAHPP*LCQQPLCL
LHLWHLSQLQPQCPLCIPHLPWKMSPPPKN*RQ
RTASCQRRSSCAETRVQCPSKSRCPTCRIRRNGN
*MGRCWSSPSHSRTRSLSLR*RFMKPQACLQGN
RSYSMRVSSSKIPTHWLTTTWPMAQSSTWPSRR
EAGGRSRQEEPAVKSLPFCLSCLPPPAPDPGAPL
RLCLACIFVSLLLSLGIQIVLQRFIPLTLSPHW
*E*LGFLSHSLESLCVRVSI*GHSFLQQPISN*
EPRDVLQDSFIVTGGTWLIEYG*YCH*SFCTLTW
VV**NSDPSCEIS*IRLVSVTCALSRLEAVVTL
HSAMWLLFEDFWVL*LNSCCPHTFYVLESYSYC
PFKTLGSSKGLPHVFNSNTGSMAGTMQTKDGWCS
PCVSGRWFPAGLESLIT*LIGSVVLGRCLIGVW
I**RFFKMYVFNKYLIVLRSCTKDLIKFK*FTL
YFYPFYPF*LM*SSCEYSCLTLE*TEAQRT*VAC
HSHTL*K*KENVCKTRHSPGF*VKAGCYL*NQA
ITGR*AMLWVLEEGESLLLTISWF*S*G*FYWE
DLTCVWPLRVPSAFYLQSFHLDLLAAPSGHLQGS
EG*DHFPRSQNQLSSHHPASPSHPESVPVLWLY
YKLLGPSHFQLD*WDVIPYPV*HLPLAWLLEVC
PRGKS*NLAS*GGLCGLRP*SEPTTGCLSMANTR
NPLSTTWVSFQEGRGSGDLGSLIFQVLLSFWEQ
WAVHGVRALTPTDWQTLQDFRG*GCDRRPGMPL
GGL*VNGPFELPSREQIQFPGAPE*ISVLARSDA
EAPLTMRPCEHQWPQAQCAAWHCTSLGPAACR*
RPSVYNTKLLCTAFLILLAIS*GLSAPFLKRGG
IPKVM*PVSALESQSSSSLPGDHTALLHQTTL*N
RNKCSCCRTAGKTGGVGLKSP*F*RLACRGLQE
L*TLRNFTFSSTHYLSTCHVLLEEASNDQNHFT
LKLIILY*RIY*MVILGYVRIYT*MEIHVLLAII
FHLSL*LDKKA*YSSTHGYTYQCIRFLEVYFLK
IKAKCKIL

Frame 2

LRGRNSLGRRHLASSSY*PSGEAVLRRHRSPTP
RRTGSPERRSHAGRTRAGGAPAAARAHGAQTAH
RRRSIFKGGFCTF*ASCGDYLPSSRGQKYC*QD
CQLCGQKRA*I*S*DPTERDQQPQVQLSEPQ*PL
PCLLPPQGQRVQGREGSVCGQEWAPVSDPADAE
RAAQLPV*LSPPTAQPLQLLHEASGTVHQDLDS
TQRFIFKAQERG*KPPRSFGSGVLPSGMGQIPGT
*EEEGRRGEGEGAGGLCSDRLA*FCGGGNSGLP
TQ*ARELPSPHHARGAGGPNPHSGAL*KVWGE*
GS*DGGRV**GG*QTGEGGGASFPAGPGHPSTRY
G*GFR**RRRAESAPTPRDTHASTSAPNSRPSH
CPQGL*SQSLQALASSPCSR*VSCVPHYWGEDP
RQQNAGTHAHWTS*PSLAGAAGSLHP*EAER**G
VRTRSGY*EQLEAVG*AAY*HLRCRGNSHW*ED
R*GGDPEARGKGDLGWPLRQHGPDPAGCPGQHH
PPGAD*GHSQGQRPGARG*H*REDWPQQAQ*NPS
TATATIFSHQHPQLGSTHHFSAPTTHNATSSSY
YSCLRSTRHAPAPNGICGPAAPRLSDRPHAAHH
PRAQNQRGAHASLGPSYYGPPPTPHDCANSLCAC
STCGTCPSSSPNAPCASPTSHGR*AHLQKTEDR
GQPHARGGVPAQKQGSSVHQSPGAQHAG*DGME
TEWAGAGLHPPTHGPGLCH*GEDS*SHRHACRET
EATV*GYLHQRFQLTGLLQHGQWRSHPPGPQGE
RREEEVDKRNLLSSPCHFASPVSHPLPQTQEPP
*GFALPAYLFRSYSVWEFKLSCRGSFP*PFPHIG
KSSWVF*ATLWNLFVLGSRFEDIHFFSSPLATE
SPGMSYRIVS**QVALG**NMADIVINHFVP*H
GLSNKTRTLLVKSVK*DLSRSPVPCPDSRQW*HC
TVLCGFSLRIFGFCN*ILAALILFMY*NHIRIA
LLKHWDPPKACPMYLTVIQEAWQAPCKPRMDGA
VPVSVGGGFLLAWNHSSPD*LALWSWAGAS*VCG
YDDVSLKCMYLTNT*LY*GHVPRI**SLNNLLS
TFIHFIHFNSCNPHVSIPV*HLSKLRHREHKLH
AIVTHCESEKRMCAKHVTVLVSE*RQAVIFRIKL
SQGDRQCCGCWRKVRACC*QFPGFKAKADFIGK
ISHVCGP*EFPVPFICSPSIWTS*LPHQVISRA
QRGETISQGHRTSSLVTTLPLPLTQSQYQFYGFI
TNCWVPPIFNLIDGMSSLILSDICLWPGC*KFA
PGARVEIWLPEVGFVVCVPKVSPLLVACPWPTP
EIP*ALPGSHSKKEEGQETWGVSYSKFFFLSGSS
GQFMVLGHSPPQTGKPCRTSVAEAVTGGQECRW
VDCE*MGPLSCPLESKSSFLELLNEYLYWLAQM
QKLH*P*GLVNISGHRPSVLLGTALV*DLQHVGS
VLVFIIQSCSAQLF*FFLQSPEDYLPHF*NEVE
YPRSCSQ*VLWKAKAAHPFLGTTLLCSTRPHYE
IGISAPVAGLLGKQVVWDLSHHNFEDLHAEGSRN
CRH*GISLSVLPTT*VLVMYS*RRPVMIRTILL
*N**YCIREYIKWLYWVMLGYILEWKYMYY*QS
YFIYPCN*TRKHNIALLMGTHTSV*DF*KFTF*K
*KQNVRS*

Sequence NM_005877

ACCTTCGCGGCCGCAACTCGCTCGGCCGCCGCCATCTTGCGAGCTCGTCGTACTGACCGAGCGGGGAGGCTGTCTTGAGGCGGCACCGCTCACCGACACC
GAGGCGGACTGGCAGCCCTGAGCGTCGCAGTCATGCCGGCCGGACCCGTGCAGGCGGTGCCCCCGCCGCCGCCCGTGCCCACGGAGCCCAAACAGCCCAC
AGAAGAAGAAGCATCTTCAAAGGAGGATTCTGCACCTTCTAAGCCAGTTGTGGGGATTATTTACCCTCCTCCAGAGGTCAGAAATATTGTTGACAAGACT
GCCAGCTTTGTGGCCAGAAACGGGCCTGAATTTGAAGCTAGGATCCGACAGAACGAGATCAACAACCCCAAGTTCAACTTTCTGAACCCCAATGACCCTT
ACCATGCCTACTACCGCCACAAGGTCAGCGAGTTCAAGGAAGGGAAGGCTCAGGAGCCGTCCGCCGCCATCCCCAAGGTCATGCAGCAGCAGCAGCAGAC
CACCCAGCAGCAGCTGCCCCAGAAGGTCCAAGCCCAAGTAATCCAAGAGACCATCGTGCCCAAAGAGCCTCCTCCTGAGTTTGAGTTCATTGCTGATCCT
CCCTCTATCTCAGCCTTCGACTTGGATGTGGTGAAGCTGAC
GGCTCAGTTTGTGGCCAGGAATGGGCGCCAGTTTCTGACCCAGCTGATGCAGAAAGAGC
AGCGCAACTACCAGTTTGACTTTCTCCGCCCACAGCACAGCCTCTTCAACTACTTCACGAAGCTAGTGGAACAGTACACCAAGATCTTGATTCCACCCAA
AGGTTTATTTTCAAAGCTCAAGAAAGAGGCTGAAAACCCCCGAGAAGTTTTGGATCAGGTGTGTTACCGAGTGGAATGGGCCAAATTCCAGGAACGTGAG
AGGAAGAAGGAAGAAGAGGAGAAGGAGAAGGAGCGGGTGGCCTATGCTCAGATCGACTGGCATGATTTTGTGGTGGTGGAAACAGTGGACTTCCAACCCA
ATGAGCAAGGGAACTTCCCTCCCCCCACCACGCCAGAGGAGCTGGGGGCCCGAATCCTCATTCAGGAGCGCTATGAAAAGTTTGGGGAGAGTGAGGAAGT
TGAGATGGAGGTCGAGTCTGATGAGGAGGATGACAAACAGGAGAAGGCGGAGGAGCCTCCTTCCCAGCTGGACCAGGACACCCAAGTACAAGATATGGAT
GAGGGTTCAGATGATGAAGAAGAAGGGCAGAAAGTGCCCCCACCCCCAGAGACACCCATGCCTCCACCTCTGCCCCCAACTCCAGACCAAGTCATTGTCC
GCAAGGATTATGATCCCAAAGCCTCCAAGCCCTTGCCTCCAGCCCCTGCTCCAGATGAGTATCTTGTGTCCCCCATTACTGGGGAGAAGATCCCCGCCAG
CAAAATGCAGGAACACATGCGCATTGGACTTCTTGACCCTCGCTGGCTGGAGCAGCGGGATCGCTCCATCCGTGAGAAGCAGAGCGATGATGAGGTGTAC
GCACCAGGTCTGGATATTGAGAGCAGCTTGAAGCAGTTGGCTGAGCGGCGTACTGACATCTTCGGTGTAGAGGAAACAGCCATTGGTAAGAAGATCGGTG
AGGAGGAGATCCAGAAGCCAGAGGAAAAGGTGACCTGGGATGGCCACTCAGGCAGCATGGCCCGGACCCAGCAGGCTGCCCAGGCCAACATCACCCTCCA
GGAGCAGATTGAGGCCATTCACAAGGCCAAAGGCCTGGTGCCAGAGGATGACACTAAAGAGAAGATTGGCCCCAGCAAGCCCAATGAAATCCCTCAACAG
CCACCGCCACCATCTTCAGCCACCAACATCCCCAGCTCGGCTCCACCCATCACTTCAGTGCCCCGACCACCCACAATGCCACCTCCAGTTCGTACTACAG
TTGTCTCCGCAGTACCCGTCATGCCCCGGCCCCCAATGGCATCTGTGGTCCGGCTGCCCCCAGGCTCAGTGATCGCCCCCATGCCGCCCATCATCCACGC
GCCCAGAATCAACGTGGTGCCCATGCCTCCCTCGGCCCCTCCTATTATGGCCCCCCGCCCACCCCCCATGATTGTGCCAACAGCCTTTGTGCCTGCTCCA
CCTGTGGCACCTGTCCCAGCTCCAGCCCCAATGCCCCCTGTGCATCCCCCACCTCCCATGGAAGATGAGCCCACCTCCAAAAAACTGAAGACAGAGGACA
GCCTCATGCCAGAGGAGGAGTTCCTGCGCAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATAAGACGGAATGGAAACTGAA
TGGGCAGGTGCTGGTCTTCACCCTCCCACTCACGGACCAGGTCTCTGTCATTAAGGTGAAGATTCATGAAGCCACAGGCATGCCTGCAGGGAAACAGAAG
CTACAGTATGAGGGTATCTTCATCAAAGATTCCAACTCACTGGCTTACTACAACATGGCCAATGGCGCAGTCATCCACCTGGCCCTCAAGGAGAGAGGCG
GGAGGAAGAAGTAGACAAGAGGAACCTGCTGTCAAGTCCCTGCCATTTTGCCTCTCCTGTCTCCCACCCCCTGCCCCAGACCCAGGAGCCCCCCTGAGGC
TTTGCCTTGCCTGCATATTTGTTTCGCTCTTACTCAGTTTGGGAATTCAAATTGTCCTGCAGAGGTTCATTCCCCTGACCCTTTCCCCACATTGGTAAGA
GTAGCTGGGTTTTCTAAGCCACTCTCTGGAATCTCTTTGTGTTAGGGTCTCGATTTGAGGACATTCATTTCTTCAGCAGCCCATTAGCAACTGAGAGCCC
AGGGATGTCCTACAGGATAGTTTCATAGTGACAGGTGGCACTTGGCTAATAGAATATGGCTGATATTGTCATTAATCATTTTGTACCTTGACATGGGTTG
TCTAATAAAACTCGGACCCTTCTTGTGAAATCAGTTAAATAAGACTTGTCTCGGTCACCTGTGCCCTGTCCAGACTCGAGGCAGTGGTAACACTGCACAG
TGCTATGTGGCTTCTCTTTGAGGATTTTTGGGTTTTGTAACTAAATTCTTGCTGCCCTCATACTTTTTATGTATTAGAATCATATTCGTATTGCCCTTTT
AAAACATTGGGATCCTCCAAAGGCCTGCCCCATGTATTTAACAGTAATACAGGAAGCATGGCAGGCACCATGCAAACCAAGGATGGATGGTGCAGTCCCT
GTGTCAGTGGGCGGTGGTTTCCTGCTGGCCTGGAATCACTCATCACCTGATTGATTGGCTCTGTGGTCCTGGGCAGGTGCCTCATAGGTGTGTGGATATG
ATGACGTTTCTTTAAAATGTATGTATTTAACAAATACTTAATTGTATTAAGGTCATGTACCAAGGATTTGATAAAGTTTAAATAATTTACTCTCTACTTT
TATCCATTTTATCCATTTTAACTCATGTAATCCTCATGTGAGTATTCCTGTTTAACACTTGAGTAAACTGAGGCACAGAGAACATAAGTTGCATGCCATA
GTCACACACTGTGAAAGTGAAAAGAGAATGTGTGCAAAACACGTCACAGTCCTGGTTTCTGAGTAAAGGCAGGCTGTTATCTTTAGAATCAAGCTATCAC
AGGGAGATAGGCAATGCTGTGGGTGTTGGAGGAAGGTGAGAGCCTGTTGCTAACAATTTCCTGGTTTTAAAGCTAAGGCTGATTTTATTGGGAAGATCTC
ACATGTGTGTGGCCCCTGAGAGTTCCCAGTGCCTTTTATTTGCAGTCCTTCCATTTGGACCTCCTAGCTGCCCCATCAGGTCATCTCCAGGGCTCAGAGG
GGTGAGACCATTTCCCAAGGTCACAGAACCAGCTCTCTAGTCACCACCCTGCCTCTCCCTCTCACCCAGAGTCAGTACCAGTTTTATGGCTTTATTACAA
ACTGCTGGGTCCCTCCCATTTTCAACTTGATTGATGGGATGTCATCCCTTATCCTGTCTGACATTTGCCTCTGGCCTGGTTGCTAGAAGTTTGCCCCAGG
GGCAAGAGTTGAAATTTGGCTTCCTGAGGTGGGCTTTGTGGTTTGCGTCCCTAAAGTGAGCCCACTACTGGTTGCTTGTCCATGGCCAACACCAGAAATC
CCCTGAGCACTACCTGGGTCTCATTCCAAGAAGGAAGAGGGTCAGGAGACCTGGGGAGTCTCATATTCCAAGTTCTTCTTTCTTTCTGGGAGCAGTGGGC
AGTTCATGGTGTTAGGGCACTCACCCCCACAGACTGGCAAACCCTGCAGGACTTCCGTGGCTGAGGCTGTGACCGGAGGCCAGGAATGCCGTTGGGTGGA
TTGTGAGTGAATGGGCCCTTTGAGCTGCCCTCTAGAGAGCAAATCCAGTTTCCTGGAGCTCCTGAATGAATATCTGTACTGGCTCGCTCAGATGCAGAAG
CTCCATTGACCATGAGGCCTTGTGAACATCAGTGGCCACAGGCCCAGTGTGCTGCTTGGCACTGCACTAGTTTAGGACCTGCAGCATGTAGGTAGCGTCC
TAGTGTTTATAATACAAAGCTGCTCTGCACAGCTTTTCTGATTCTTCTTGCAATCTCCTGAGGATTATCTGCCCCATTTTTAAAACGAGGTGGAATACCC
AAGGTCATGTAGCCAGTGAGTGCTCTGGAAAGCCAAAGCAGCTCATCCCTTCCTGGGGACCACACTGCTCTGCTCCACCAGACCACACTATGAAATAGGA
ATAAGTGCTCCTGTTGCAGGACTGCTGGGAAAACAGGTGGTGTGGGACTTAAGTCACCATAATTTTGAAGACTTGCATGCAGAGGGCTCCAGGAATTGTA
GACATTAAGGAATTTCACTTTCAGTTCTACCCACTACTTAAGTACTTGTCATGTACTCTTAGAGGAGGCCAGTAATGATCAGAACCATTTTACTTTAAAA
TTAATAATATTGTATTAGAGAATATATTAAATGGTTATATTGGGTTATGTTAGGATATATACTTGAATGGAAATACATGTACTATTAGCAATCATATTTC
ATTTATCCCTGTAATTAGACAAGAAAGCATAATATAGCTCTACTCATGGGTACACATACCAGTGTATAAGATTTTTAGAAGTTTACTTTTTAAAAATAAA
AGCAAAATGTAAGATCTTAA
  • refseq_SF3A1.F1 (forward primer)
  • refseq_SF3A1.R1 (reverse primer)
  • Alternative donor and acceptor

Frame 0

TFAAATRSAAAILRARRTDRAGRLS*GGTAHRH
RGGLAALSVAVMPAGPVQAVPPPPPVPTEPKQPT
EEEASSKEDSAPSKPVVGIIYPPPEVRNIVDKT
ASFVARNGPEFEARIRQNEINNPKFNFLNPNDP
YHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQT
TQQQLPQKVQAQVIQETIVPKEPPPEFEFIADP
PSISAFDLDVVKLT
AQFVARNGRQFLTQLMQKE
QRNYQFDFLRPQHSLFNYFTKLVEQYTKILIPPK
GLFSKLKKEAENPREVLDQVCYRVEWAKFQERE
RKKEEEEKEKERVAYAQIDWHDFVVVETVDFQP
NEQGNFPPPTTPEELGARILIQERYEKFGESEEV
EMEVESDEEDDKQEKAEEPPSQLDQDTQVQDMD
EGSDDEEEGQKVPPPPETPMPPPLPPTPDQVIV
RKDYDPKASKPLPPAPAPDEYLVSPITGEKIPAS
KMQEHMRIGLLDPRWLEQRDRSIREKQSDDEVY
APGLDIESSLKQLAERRTDIFGVEETAIGKKIG
EEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQ
EQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQ
PPPPSSATNIPSSAPPITSVPRPPTMPPPVRTT
VVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHA
PRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAP
PVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTED
SLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLN
GQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQK
LQYEGIFIKDSNSLAYYNMANGAVIHLALKERG
GRKK*TRGTCCQVPAILPLLSPTPCPRPRSPPEA
LPCLHICFALTQFGNSNCPAEVHSPDPFPTLVR
VAGFSKPLSGISLC*GLDLRTFISSAAH*QLRA
QGCPTG*FHSDRWHLANRIWLILSLIILYLDMGC
LIKLGPFL*NQLNKTCLGHLCPVQTRGSGNTAQ
CYVASL*GFLGFVTKFLLPSYFLCIRIIFVLPF
*NIGILQRPAPCI*Q*YRKHGRHHANQGWMVQSL
CQWAVVSCWPGITHHLIDWLCGPGQVPHRCVDM
MTFL*NVCI*QILNCIKVMYQGFDKV*IIYSLL
LSILSILTHVILM*VFLFNT*VN*GTENISCMP*
SHTVKVKRECVQNTSQSWFLSKGRLLSLESSYH
REIGNAVGVGGR*EPVANNFLVLKLRLILLGRS
HMCVAPESSQCLLFAVLPFGPPSCPIRSSPGLRG
VRPFPKVTEPAL*SPPCLSLSPRVSTSFMALLQ
TAGSLPFST*LMGCHPLSCLTFASGLVARSLPQ
GQELKFGFLRWALWFASLK*AHYWLLVHGQHQKS
PEHYLGLIPRRKRVRRPGESHIPSSSFFLGAVG
SSWC*GTHPHRLANPAGLPWLRL*PEARNAVGW
IVSEWAL*AAL*RANPVSWSS*MNICTGSLRCRS
SIDHEAL*TSVATGPVCCLALH*FRTCSM*VAS
*CL*YKAALHSFSDSSCNLLRIICPIFKTRWNT
QGHVASECSGKPKQLIPSWGPHCSAPPDHTMK*E
*VLLLQDCWENRWCGT*VTIILKTCMQRAPGIV
DIKEFHFQFYPLLKYLSCTLRGGQ**SEPFYFK
INNIVLENILNGYIGLC*DIYLNGNTCTISNHIS
FIPVIRQESII*LYSWVHIPVYKIFRSLLFKNK
SKM*DL

Frame 1

PSRPQLARPPPSCELVVLTERGGCLEAAPLTDT
EADWQP*ASQSCRPDPCRRCPRRRPCPRSPNSP
QKKKHLQRRILHLLSQLWGLFTLLQRSEILLTRL
PALWPETGLNLKLGSDRTRSTTPSSTF*TPMTL
TMPTTATRSASSRKGRLRSRPPPSPRSCSSSSR
PPSSSCPRRSKPK*SKRPSCPKSLLLSLSSLLIL
PLSQPSTWMW*S*
RLSLWPGMGASF*PS*CRKS
SATTSLTFSAHSTASSTTSRS*WNSTPRS*FHP
KVYFQSSRKRLKTPEKFWIRCVTEWNGPNSRNVR
GRRKKRRRRRSGWPMLRSTGMILWWWKQWTSNP
MSKGTSLPPPRQRSWGPESSFRSAMKSLGRVRK
LRWRSSLMRRMTNRRRRRSLLPSWTRTPKYKIWM
RVQMMKKKGRKCPHPQRHPCLHLCPQLQTKSLS
ARIMIPKPPSPCLQPLLQMSILCPPLLGRRSPP
AKCRNTCALDFLTLAGWSSGIAPSVRSRAMMRCT
HQVWILRAA*SSWLSGVLTSSV*RKQPLVRRSV
RRRSRSQRKR*PGMATQAAWPGPSRLPRPTSPS
RSRLRPFTRPKAWCQRMTLKRRLAPASPMKSLNS
HRHHLQPPTSPARLHPSLQCPDHPQCHLQFVLQ
LSPQYPSCPGPQWHLWSGCPQAQ*SPPCRPSST
RPESTWCPCLPRPLLLWPPAHPP*LCQQPLCLLH
LWHLSQLQPQCPLCIPHLPWKMSPPPKN*RQRT
ASCQRRSSCAETRVQCPSKSRCPTCRIRRNGN*
MGRCWSSPSHSRTRSLSLR*RFMKPQACLQGNRS
YSMRVSSSKIPTHWLTTTWPMAQSSTWPSRREA
GGRSRQEEPAVKSLPFCLSCLPPPAPDPGAPLR
LCLACIFVSLLLSLGIQIVLQRFIPLTLSPHW*E
*LGFLSHSLESLCVRVSI*GHSFLQQPISN*EP
RDVLQDSFIVTGGTWLIEYG*YCH*SFCTLTWV
V**NSDPSCEIS*IRLVSVTCALSRLEAVVTLHS
AMWLLFEDFWVL*LNSCCPHTFYVLESYSYCPF
KTLGSSKGLPHVFNSNTGSMAGTMQTKDGWCSP
CVSGRWFPAGLESLIT*LIGSVVLGRCLIGVWI*
*RFFKMYVFNKYLIVLRSCTKDLIKFK*FTLYF
YPFYPF*LM*SSCEYSCLTLE*TEAQRT*VACH
SHTL*K*KENVCKTRHSPGF*VKAGCYL*NQAIT
GR*AMLWVLEEGESLLLTISWF*S*G*FYWEDL
TCVWPLRVPSAFYLQSFHLDLLAAPSGHLQGSE
G*DHFPRSQNQLSSHHPASPSHPESVPVLWLYYK
LLGPSHFQLD*WDVIPYPV*HLPLAWLLEVCPR
GKS*NLAS*GGLCGLRP*SEPTTGCLSMANTRN
PLSTTWVSFQEGRGSGDLGSLIFQVLLSFWEQWA
VHGVRALTPTDWQTLQDFRG*GCDRRPGMPLGG
L*VNGPFELPSREQIQFPGAPE*ISVLARSDAE
APLTMRPCEHQWPQAQCAAWHCTSLGPAACR*RP
SVYNTKLLCTAFLILLAIS*GLSAPFLKRGGIP
KVM*PVSALESQSSSSLPGDHTALLHQTTL*NR
NKCSCCRTAGKTGGVGLKSP*F*RLACRGLQEL*
TLRNFTFSSTHYLSTCHVLLEEASNDQNHFTLK
LIILY*RIY*MVILGYVRIYT*MEIHVLLAIIF
HLSL*LDKKA*YSSTHGYTYQCIRFLEVYFLKIK
AKCKIL

Frame 2

LRGRNSLGRRHLASSSY*PSGEAVLRRHRSPTP
RRTGSPERRSHAGRTRAGGAPAAARAHGAQTAH
RRRSIFKGGFCTF*ASCGDYLPSSRGQKYC*QD
CQLCGQKRA*I*S*DPTERDQQPQVQLSEPQ*PL
PCLLPPQGQRVQGREGSGAVRRHPQGHAAAAAD
HPAAAAPEGPSPSNPRDHRAQRASS*V*VHC*S
SLYLSLRLGCGEAD
GSVCGQEWAPVSDPADAERA
AQLPV*LSPPTAQPLQLLHEASGTVHQDLDSTQ
RFIFKAQERG*KPPRSFGSGVLPSGMGQIPGT*
EEEGRRGEGEGAGGLCSDRLA*FCGGGNSGLPTQ
*ARELPSPHHARGAGGPNPHSGAL*KVWGE*GS
*DGGRV**GG*QTGEGGGASFPAGPGHPSTRYG
*GFR**RRRAESAPTPRDTHASTSAPNSRPSHCP
QGL*SQSLQALASSPCSR*VSCVPHYWGEDPRQ
QNAGTHAHWTS*PSLAGAAGSLHP*EAER**GV
RTRSGY*EQLEAVG*AAY*HLRCRGNSHW*EDR*
GGDPEARGKGDLGWPLRQHGPDPAGCPGQHHPP
GAD*GHSQGQRPGARG*H*REDWPQQAQ*NPST
ATATIFSHQHPQLGSTHHFSAPTTHNATSSSYYS
CLRSTRHAPAPNGICGPAAPRLSDRPHAAHHPR
AQNQRGAHASLGPSYYGPPPTPHDCANSLCACS
TCGTCPSSSPNAPCASPTSHGR*AHLQKTEDRGQ
PHARGGVPAQKQGSSVHQSPGAQHAG*DGMETE
WAGAGLHPPTHGPGLCH*GEDS*SHRHACRETE
ATV*GYLHQRFQLTGLLQHGQWRSHPPGPQGERR
EEEVDKRNLLSSPCHFASPVSHPLPQTQEPP*G
FALPAYLFRSYSVWEFKLSCRGSFP*PFPHIGK
SSWVF*ATLWNLFVLGSRFEDIHFFSSPLATESP
GMSYRIVS**QVALG**NMADIVINHFVP*HGL
SNKTRTLLVKSVK*DLSRSPVPCPDSRQW*HCT
VLCGFSLRIFGFCN*ILAALILFMY*NHIRIALL
KHWDPPKACPMYLTVIQEAWQAPCKPRMDGAVP
VSVGGGFLLAWNHSSPD*LALWSWAGAS*VCGY
DDVSLKCMYLTNT*LY*GHVPRI**SLNNLLSTF
IHFIHFNSCNPHVSIPV*HLSKLRHREHKLHAI
VTHCESEKRMCAKHVTVLVSE*RQAVIFRIKLS
QGDRQCCGCWRKVRACC*QFPGFKAKADFIGKIS
HVCGP*EFPVPFICSPSIWTS*LPHQVISRAQR
GETISQGHRTSSLVTTLPLPLTQSQYQFYGFIT
NCWVPPIFNLIDGMSSLILSDICLWPGC*KFAPG
ARVEIWLPEVGFVVCVPKVSPLLVACPWPTPEI
P*ALPGSHSKKEEGQETWGVSYSKFFFLSGSSG
QFMVLGHSPPQTGKPCRTSVAEAVTGGQECRWVD
CE*MGPLSCPLESKSSFLELLNEYLYWLAQMQK
LH*P*GLVNISGHRPSVLLGTALV*DLQHVGSV
LVFIIQSCSAQLF*FFLQSPEDYLPHF*NEVEYP
RSCSQ*VLWKAKAAHPFLGTTLLCSTRPHYEIG
ISAPVAGLLGKQVVWDLSHHNFEDLHAEGSRNC
RH*GISLSVLPTT*VLVMYS*RRPVMIRTILL*N
**YCIREYIKWLYWVMLGYILEWKYMYY*QSYF
IYPCN*TRKHNIALLMGTHTSV*DF*KFTF*K*
KQNVRS*

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