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Sequences

Sequence NM_017508

GGGATAGGAAAGATGAAAGGTCATGGTGAGCTTCAAGGACATGAAAGGTTGTTGTCTCATGTAACAATAGTAGATTGTTTTTTTTCCTAATATTTCTAGC CAGCCCCTAAGTCAGGTGATGGAACAAATACCTACAGTTTAGTCAGGTGAAACAGGAGTGGGTGGAGGAAGGAAAGAAGAAAAATGGGAAGAATGTCTTC CAAGCAAGCCACCTCTCCATTTGCCTGTGCAGCTGATGGAGAGGATGCAATGACCCAGGATTTAACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACAT GTGGCCTCCCATCTGCCTCTGCACCCCATAATGCACAACAAACCTCACTCTGAGGAGCTACCAACACTTGTCAGTACCATTCAACAAGATGCTGACTGGG ACAGCGTTCTGTCATCTCAGCAAAGAATGGAATCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAAATACCTCTACCTCACCACATAAGCCTGA CGAAGGGAGTCGGGACCGTGAGATAATGACCAGTGTTACTTTTGGAACCCCAGAGCGCCGCAAAGGGAGTCTTGCCGATGTGGTGGACACACTGAAACAG AAGAAGCTTGAGGAAATGACTCGGACTGAACAAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAAAAATGGAAAGACTAAATA CCAGTGAACTTCTTGGAGAAATTAAAGGTACACCTGAGAGCCTGGCAGAAAAAGAACGGCAGCTCTCCACCATGATTACCCAGCTGATCAGTTTACGGGA GCAGCTACTGGCAGCGCATGATGAACAGAAAAAACTGGCAGCGTCACAAATTGAGAAACAACGGCAGCAAATGGACCTTGCTCGCCAACAGCAAGAACAG ATTGCGAGACAACAGCAGCAACTTCTGCAACAGCAGCACAAAATTAATCTCCTGCAGCAACAGATCCAGGTTCAGGGTCACATGCCTCCGCTCATGATCC CAATTTTTCCACATGACCAGCGGACTCTGGCAGCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAACCAGGTGATAACTA CCCCGTACAGTTCATTCCATCAACAATGGCAGCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTCCAGCAGCTCTATGCCGCTCAGCTGGCCAGCATG CAGGTGTCACCTGGAGCAAAGATGCCATCAACTCCACAGCCACCAAACACAGCAGGGACGGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCA GCCCTGTAACTCAAGTTAAGGATGAAGCAGCAGCACAGCCTCTGAATCTCTCATCCCGACCCAAGACAGCAGAGCCTGTAAAGTCCCCAACGTCTCCCAC CCAGAACCTCTTCCCAGCCAGCAAAACCAGCCCTGTCAATCTGCCAAACAAAAGCAGCATCCCTAGCCCCATTGGAGGAAGCCTGGGAAGAGGATCCTCT TTAGGTAAATGGAAAAGTCAACACCAGGAAGAGACTTACGAATTAGATATCCTATCTAGTCTCAACTCCCCTGCCCTTTTTGGGGATCAGGATACAGTGA TGAAAGCCATTCAGGAGGCGCGGAAGATGCGAGAGCAGATCCAGCGGGAGCAACAGCAGCAACAGCCACATGGTGTTGACGGGAAACTGTCCTCCATAAA TAATATGGGGCTGAACAGCTGCAGGAATGAAAAGGAAAGAACGCGCTTTGAGAATTTGGGGCCCCAGTTAACGGGAAAGTCAAATGAAGATGGAAAACTG GGCCCAGGTGTCATCGACCTTACTCGGCCAGAAGATGCAGAGGGAAGTAAAGCAATGAATGGCTCTGCAGCTAAACTACAGCAGTATTATTGTTGGCCAA CAGGAGGTGCCACTGTGGCTGAAGCACGAGTCTACAGGGACGCCCGCGGCCGTGCCAGCAGCGAGCCACACATTAAGCGACCAATGAATGCATTCATGGT TTGGGCAAAGGATGAGAGGAGAAAAATCCTTCAGGCCTTCCCCGACATGCATAACTCCAACATTAGCAAAATCTTAGGATCTCGCTGGAAATCAATGTCC AACCAGGAGAAGCAACCTTATTATGAAGAGCAGGCCCGGCTAAGCAAGATCCACTTAGAGAAGTACCCAAACTATAAATACAAACCCCGACCGAAACGCA CCTGCATTGTTGATGGCAAAAAGCTTCGGATTGGGGAGTATAAGCAACTGATGAGGTCTCGGAGACAGGAGATGAGGCAGTTCTTTACTGTGGGGCAACA GCCTCAGATTCCAATCACCACAGGAACAGGTGTTGTGTATCCTGGTGCTATCACTATGGCAACTACCACACCATCGCCTCAGATGACATCTGACTGCTCT AGCACCTCGGCCAGCCCGGAGCCCAGCCTCCCGGTCATCCAGAGCACTTATGGTATGAAGACAGATGGCGGAAGCCTAGCTGGAAATGAAATGATCAATG GAGAGGATGAAATGGAAATGTATGATGACTATGAAGATGACCCCAAATCAGACTATAGCAGTGAAAATGAAGCCCCGGAGGCTGTCAGTGCCAACTGAGG AGTTTTTGTTTGCTGAATTAAAGTACTCTGACATTTCACCCCCCTCCCCAACAAAGAGTTATTAAAGAGCCCGCATGCATTTGTGGCTCCACAATTACAT CAGCAGAATGGTCTTAATTGTTTCGTAAAGTGTGAGACAGATTAAGTTTTCCCTGATTTTTCATGAACTTGAGTTTTTTGTTGTTATTGTTATTGTTGTT GTTGTTGTTTTTTTAATTTAGGTGAAGACATATTAAATATGAGACACCAGGACTTGAAACTTATCTCAACCCGTAGATGTCTTACAAGTCTTATATTTTT GTCTTACTTTTTTTTTCTTTTGGATGTTGATAAAGGTTTAAGTTACTGTTTTAGATGGGGTTAAACATTCTCACTCAGGTATGCTGTGCCGGCCTACAGG TTGTGAATGTGTTTTTATTCTGAATTATTTTAGAAAACAACTGAGGATTTCATATTGTGAAACAGAACAAGTCCACGGCGTGTGCAGCTGCATGTAGAGC ATATTCAAAAGGCCTCGGAATTCCAATTTTCCATTTGTAGAGTTAAACTTTGAATGTGCCAAACTTTTTCGTAACTTTTGAATCTTAATATTTTGAAAGT CTTAAAGGAGACACTGCAAAGTCTTAGACAATCTTTGGCATCTTAAAATAAAATAGCAAACCAACATTTTTTTTTCCAGAAAATGGTAAGGTACTCAGGA ATCTGGAGACAAGATATTGTAAGGAATGAACAAGGTTGCCACAGTGCATGGACCCAATTGTGTTTGCCTGTTGACGTGCCATCAGTGCGTGATGTGGTAT GACATACACACACCAGAGCAACCGCCACACCAGATATCGACAGAGTGGTCTTCTCTGCCTGAGACCACCTCTCACTACATCCATTATCCCTTTGCCTTTA ACCCTGACATTCAGTCTTAACACATTTTATCTTAAATAATTTATTCATTCCAGAATGTCAAGGGTCCACTTGCTATTTATTTTTTTTCAATTGTTGGTGG CATTAATTTAATAATTCTTGTTTTTCACCTTCCTTCCCCGAAGAACTTTTCCGTCCTTTTCACCTCCTTCTCCTGTGTACATAGTGATTTTATGTCCCCA GAACGCCTGGAAGCATTTCTGAAACCAAGATATTATTAAAAACCTATTATTGTTTTTAATCATGAGTATGTATCTGGCTGCAGGGCTGTGTATTGGGATA TAGGTATATAGTCTTACACTTAAACAGGTATGCCCCTGAGGTTCACTGTGACCTCAAGTCTTTTGCCAGAATTTTCCCCTAATTCAGTTCACAAGTGGTA GGGTCTGCATCAGTGGCATTTCCCCCTGAATTCCATTCAGCAGCAAGGTTCAACAGTGGTGACTGCCAGGCAGGAGAGTCCTGCGGCCAAACCTGAAGCC CAAGGCTCGTGGGCCATGCAGGAATCTCAGTGAAGCTGTCATGGGCTGGCACCTTTACACTGAGTTGCCTTGTCCCAGCTGGCACATCTAGGGAGTTCAT TGCAAAATCCCCAGGATGCAAAAAGCCACATGACAGCCTCAGAGCAAAGATGGTGGCAAATAGTCATGATACATCTAGAGAATGAAAGAAAACTGTAAGG GAGGAGAAGGAGGGGAATACATTCCCTATATGGGATGTTCCTACTGTTAACTCTGGGGAACAGATAGCTCCGGGGGCAGCAGATGAGTTCCTCTGGCTGA CTCTATCTGTAGCCACATGGGGACCTGCCTACGTGTGAACAAAATGAACTGCACTTATCACACAAGGATTTCTTTGAAGACATGCTACTGGGGTGGGAAG CAGTGAGGTTTTATTCCCCATCTCCTAACTACAGGGAGCTCTGCCATGTCATTTTGGCCTTCCTGAAACTAGGACAGGTTGTCTATCGGGGGGCTTCCCC CAGAGAGGTTTAGTGGGAGAATGTCAGTGAATGGGATAGTTCACCTCATGGGACAACCCAGAATCTGATCACCAGGACATAGGAATGGCCCCATCAGATT TCTTGAGCCATTTTGTCACTTGGAAGAAAATAGTGTACCTTTGTATTTATTTAAGAGTGCTCAAGGCCTAATAGCAATAAACAGGTCTAGCCAAGAAATT ACAAGCTATTCTGTTAGCTGGGAGTGCTCTCTATAAGCTGATTAAGGTACTGATAGGAACTCTTTGTTATTCATGTTGGTTGGGGATTAGAAATTTGTTT TTGTACATTTATTTCAAATGAGGAGGAGGTCATTTTTTCTCTCAAAAAATGAGTATTTATTATTGTCTTACTGATTTCTTTGATTATATACCTCTCCTCC TCAGTTCACTCTTGTTTTTTTTCTTTCTCTTTGGCTTTTGCTTTTGCTCTCTCTCACTTCTTTCTTATTTTGTTGCATTGGTAGAGTGTTGTATGGCTAG CATTGTATTGTATGTAATTAATTTTGCACAAAAGCAAACATTTAGCATAGTAGGTTAATTTTGTTTGTTTTTATGACCATGCCAAAATAATATTCTGGGC TGGTGGAGAACAAAGGACTGTTCTTTAGGACTGAAACTTGATTTTGCTCATAGTAAGT

refseq_SOX6.F4 (forward primer)
refseq_SOX6.R4 (reverse primer)
Single exon skipping

Frame 0

GIGKMKGHGELQGHERLLSHVTIVDCFFS*YF* PAPKSGDGTNTYSLVR*NRSGWRKERRKMGRMSS KQATSPFACAADGEDAMTQDLTSREKEEGSDQH VASHLPLHPIMHNKPHSEELPTLVSTIQQDADW DSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPD EGSRDREIMTSVTFGTPERRKGSLADVVDTLKQ KKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLN TSELLGEIKGTPESLAEKERQLSTMITQLISLRE QLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQ IARQQQQLLQQQHKINLLQQQIQVQGHMPPLMI PIFPHDQRTLAAAAAAQQGFLFPPGITYKPGDNY PVQFIPSTMAAAAASGLSPLQLQQLYAAQLASM QVSPGAKMPSTPQPPNTAGTVSPTGIKNEKRGT SPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPT QNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSS LGKWKSQHQEETYELDILSSLNSPALFGDQDTV MKAIQEARKMREQIQREQQQQQPHGVDGKLSSIN NMGLNSCRNEKERTRFENLGPQLTGKSNEDGKL GPGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWP TGGATVAEARVYRDARGRASSEPHIKRPMNAFMV WAKDERRKILQAFPDMHNSNISKILGSRWKSMS NQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKR TCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQ PQIPITTGTGVVYPGAITMATTTPSPQMTSDCS STSASPEPSLPVIQSTYGMKTDGGSLAGNEMIN GEDEMEMYDDYEDDPKSDYSSENEAPEAVSAN*G VFVC*IKVL*HFTPLPNKELLKSPHAFVAPQLH QQNGLNCFVKCETD*VFPDFS*T*VFCCYCYCC CCCFFNLGEDILNMRHQDLKLISTRRCLTSLIFL SYFFFLLDVDKGLSYCFRWG*TFSLRYAVPAYR L*MCFYSELF*KTTEDFIL*NRTSPRRVQLHVE HIQKASEFQFSICRVKL*MCQTFS*LLNLNILKV LKETLQSLRQSLAS*NKIANQHFFFQKMVRYSG IWRQDIVRNEQGCHSAWTQLCLPVDVPSVRDVV *HTHTRATATPDIDRVVFSA*DHLSLHPLSLCL* P*HSVLTHFILNNLFIPECQGSTCYLFFFNCWW H*FNNSCFSPSFPEELFRPFHLLLLCT**FYVP RTPGSISETKILLKTYYCF*S*VCIWLQGCVLGY RYIVLHLNRYAPEVHCDLKSFARIFP*FSSQVV GSASVAFPPEFHSAARFNSGDCQAGESCGQT*S PRLVGHAGISVKLSWAGTFTLSCLVPAGTSREFI AKSPGCKKPHDSLRAKMVANSHDTSRE*KKTVR EEKEGNTFPIWDVPTVNSGEQIAPGAADEFLWL TLSVATWGPAYV*TK*TALITQGFL*RHATGVGS SEVLFPIS*LQGALPCHFGLPETRTGCLSGGFP QRGLVGECQ*MG*FTSWDNPESDHQDIGMAPSD FLSHFVTWKKIVYLCIYLRVLKA**Q*TGLAKKL QAILLAGSALYKLIKVLIGTLCYSCWLGIRNLF LYIYFK*GGGHFFSQKMSIYYCLTDFFDYIPLL LSSLLFFFFLFGFCFCSLSLLSYFVALVECCMAS IVLYVINFAQKQTFSIVG*FCLFL*PCQNNILG WWRTKDCSLGLKLDFAHSK

Frame 1

G*ER*KVMVSFKDMKGCCLM*Q**IVFFPNISS QPLSQVMEQIPTV*SGETGVGGGRKEEKWEECL PSKPPLHLPVQLMERMQ*PRI*PQGKRKRAVINM WPPICLCTP*CTTNLTLRSYQHLSVPFNKMLTG TAFCHLSKEWNQRIISYVPYIPSEIPLPHHISL TKGVGTVR**PVLLLEPQSAAKGVLPMWWTH*NR RSLRK*LGLNKRIPPAWKNYFQKIGRKKWKD*I PVNFLEKLKVHLRAWQKKNGSSPP*LPS*SVYG SSYWQRMMNRKNWQRHKLRNNGSKWTLLANSKNR LRDNSSNFCNSSTKLISCSNRSRFRVTCLRS*S QFFHMTSGLWQQLLLPNRDSSSPLE*HTNQVIT TPYSSFHQQWQLLLLLDSALYSSSSSMPLSWPAC RCHLEQRCHQLHSHQTQQGRSHLLG*KMKREGP AL*LKLRMKQQHSL*ISHPDPRQQSL*SPQRLP PRTSSQPAKPALSICQTKAASLAPLEEAWEEDPL *VNGKVNTRKRLTN*ISYLVSTPLPFLGIRIQ* *KPFRRRGRCESRSSGSNSSNSHMVLTGNCPP* IIWG*TAAGMKRKERALRIWGPS*RESQMKMENW AQVSSTLLGQKMQREVKQ*MALQLNYSSIIVGQ QEVPLWLKHESTGTPAAVPAASHTLSDQ*MHSW FGQRMRGEKSFRPSPTCITPTLAKS*DLAGNQCP TRRSNLIMKSRPG*ARST*RSTQTINTNPDRNA PALLMAKSFGLGSISN**GLGDRR*GSSLLWGN SLRFQSPQEQVLCILVLSLWQLPHHRLR*HLTAL APRPARSPASRSSRALMV*RQMAEA*LEMK*SM ERMKWKCMMTMKMTPNQTIAVKMKPRRLSVPTE EFLFAELKYSDISPPSPTKSY*RARMHLWLHNYI SRMVLIVS*SVRQIKFSLIFHELEFFVVIVIVV VVVFLI*VKTY*I*DTRT*NLSQPVDVLQVLYF CLTFFFFWMLIKV*VTVLDGVKHSHSGMLCRPTG CECVFILNYFRKQLRISYCETEQVHGVCSCM*S IFKRPRNSNFPFVELNFECAKLFRNF*ILIF*K S*RRHCKVLDNLWHLKIK*QTNIFFSRKW*GTQE SGDKIL*GMNKVATVHGPNCVCLLTCHQCVMWY DIHTPEQPPHQISTEWSSLPETTSHYIHYPFAF NPDIQS*HILS*IIYSFQNVKGPLAIYFFSIVGG INLIILVFHLPSPKNFSVLFTSFSCVHSDFMSP ERLEAFLKPRYY*KPIIVFNHEYVSGCRAVYWD IGI*SYT*TGMPLRFTVTSSLLPEFSPNSVHKW* GLHQWHFPLNSIQQQGSTVVTARQESPAAKPEA QGSWAMQESQ*SCHGLAPLH*VALSQLAHLGSS LQNPQDAKSHMTASEQRWWQIVMIHLENERKL*G RRRRGIHSLYGMFLLLTLGNR*LRGQQMSSSG* LYL*PHGDLPTCEQNELHLSHKDFFEDMLLGWE AVRFYSPSPNYRELCHVILAFLKLGQVVYRGASP REV*WENVSEWDSSPHGTTQNLITRT*EWPHQI S*AILSLGRK*CTFVFI*ECSRPNSNKQV*PRN YKLFC*LGVLSIS*LRY**ELFVIHVGWGLEICF CTFISNEEEVIFSLKK*VFIIVLLISLIIYLSS SVHSCFFSFSLAFAFALSHFFLILLHW*SVVWL ALYCM*LILHKSKHLA**VNFVCFYDHAKIIFWA GGEQRTVL*D*NLILLIVS

Frame 2

DRKDERSW*ASRT*KVVVSCNNSRLFFFLIFLA SP*VR*WNKYLQFSQVKQEWVEEGKKKNGKNVF QASHLSICLCS*WRGCNDPGFNLKGKGRGQ*ST CGLPSASAPHNAQQTSL*GATNTCQYHSTRC*LG QRSVISAKNGIRE**VMFPIFLPKYLYLTT*A* RRESGP*DNDQCYFWNPRAPQRESCRCGGHTET EEA*GNDSD*TRGFLLHGKTTFKRLEGKNGKTKY Q*TSWRN*RYT*EPGRKRTAALHHDYPADQFTG AATGSA**TEKTGSVTN*ETTAANGPCSPTART DCETTAATSATAAQN*SPAATDPGSGSHASAHDP NFST*PADSGSSCCCPTGIPLPPWNNIQTR**L PRTVHSINNGSCCCFWTQPFTAPAALCRSAGQH AGVTWSKDAINSTATKHSRDGLTYWDKK*KERDQ PCNSS*G*SSSTASESLIPTQDSRACKVPNVSH PEPLPSQQNQPCQSAKQKQHP*PHWRKPGKRIL FR*MEKSTPGRDLRIRYPI*SQLPCPFWGSGYSD ESHSGGAEDARADPAGATAATATWC*RETVLHK *YGAEQLQE*KGKNAL*EFGAPVNGKVK*RWKT GPRCHRPYSARRCRGK*SNEWLCS*TTAVLLLAN RRCHCG*STSLQGRPRPCQQRATH*ATNECIHG LGKG*EEKNPSGLPRHA*LQH*QNLRISLEINV QPGEATLL*RAGPAKQDPLREVPKL*IQTPTETH LHC*WQKASDWGV*ATDEVSETGDEAVLYCGAT ASDSNHHRNRCCVSWCYHYGNYHTIASDDI*LL *HLGQPGAQPPGHPEHLWYEDRWRKPSWK*NDQW RG*NGNV**L*R*PQIRL*Q*K*SPGGCQCQLR SFCLLN*STLTFHPPPQQRVIKEPACICGSTIT SAEWS*LFRKV*DRLSFP*FFMNLSFLLLLLLLL LLFF*FR*RHIKYETPGLETYLNP*MSYKSYIF VLLFFSFGC**RFKLLF*MGLNILTQVCCAGLQ VVNVFLF*IILENN*GFHIVKQNKSTACAAACRA YSKGLGIPIFHL*S*TLNVPNFFVTFES*YFES LKGDTAKS*TIFGILK*NSKPTFFFPENGKVLR NLETRYCKE*TRLPQCMDPIVFAC*RAISA*CGM TYTHQSNRHTRYRQSGLLCLRPPLTTSIIPLPL TLTFSLNTFYLK*FIHSRMSRVHLLFIFFQLLV ALI**FLFFTFLPRRTFPSFSPPSPVYIVILCPQ NAWKHF*NQDIIKNLLLFLIMSMYLAAGLCIGI *VYSLTLKQVCP*GSL*PQVFCQNFPLIQFTSG RVCISGISP*IPFSSKVQQW*LPGRRVLRPNLKP KARGPCRNLSEAVMGWHLYTELPCPSWHI*GVH CKIPRMQKAT*QPQSKDGGK*S*YI*RMKENCK GGEGGEYIPYMGCSYC*LWGTDSSGGSR*VPLAD SICSHMGTCLRVNKMNCTYHTRISLKTCYWGGK Q*GFIPHLLTTGSSAMSFWPS*N*DRLSIGGLP PERFSGRMSVNGIVHLMGQPRI*SPGHRNGPIRF LEPFCHLEENSVPLYLFKSAQGLIAINRSSQEI TSYSVSWECSL*AD*GTDRNSLLFMLVGD*KFV FVHLFQMRRRSFFLSKNEYLLLSY*FL*LYTSPP QFTLVFFLSLWLLLLLSLTSFLFCCIGRVLYG* HCIVCN*FCTKANI*HSRLILFVFMTMPK*YSG LVENKGLFFRTET*FCS**

Sequence NM_033326

AAAGGCAATTTACCAGTGATTTCTGGGTGCTGGGGCTGATATTTTTTGTGCATATTTAAGAATGTCTTCCAAGCAAGCCACCTCTCCATTTGCCTGTGCA GCTGATGGAGAGGATGCAATGACCCAGGATTTAACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACATGTGGCCTCCCATCTGCCTCTGCACCCCATAA TGCACAACAAACCTCACTCTGAGGAGCTACCAACACTTGTCAGTACCATTCAACAAGATGCTGACTGGGACAGCGTTCTGTCATCTCAGCAAAGAATGGA ATCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAAATACCTCTACCTCACCACATAAGCCTGACGAAGGGAGTCGGGACCGTGAGATAATGACC AGTGTTACTTTTGGAACCCCAGAGCGCCGCAAAGGGAGTCTTGCCGATGTGGTGGACACACTGAAACAGAAGAAGCTTGAGGAAATGACTCGGACTGAAC AAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAAAAATGGAAAGACTAAATACCAGTGAACTTCTTGGAGAAATTAAAGGTAC ACCTGAGAGCCTGGCAGAAAAAGAACGGCAGCTCTCCACCATGATTACCCAGCTGATCAGTTTACGGGAGCAGCTACTGGCAGCGCATGATGAACAGAAA AAACTGGCAGCGTCACAAATTGAGAAACAACGGCAGCAAATGGACCTTGCTCGCCAACAGCAAGAACAGATTGCGAGACAACAGCAGCAACTTCTGCAAC AGCAGCACAAAATTAATCTCCTGCAGCAACAGATCCAGGTTCAGGGTCACATGCCTCCGCTCATGATCCCAATTTTTCCACATGACCAGCGGACTCTGGC AGCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAACCAGGTGATAACTACCCCGTACAGTTCATTCCATCAACAATGGCA GCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTCCAGAAGGGTCATGTCTCCCACCCACAAATTAACCAAAGGCTAAAGGGCCTAAGTGACCGTTTTG GCAGGAATTTGGACACCTTTGAACATGGTGGTGGCCACTCTTACAACCACAAACAGATTGAGCAGCTCTATGCCGCTCAGCTGGCCAGCATGCAGGTGTC ACCTGGAGCAAAGATGCCATCAACTCCACAGCCACCAAACACAGCAGGGACGGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCAGCCCTGTA ACTCAAGTTAAGGATGAAGCAGCAGCACAGCCTCTGAATCTCTCATCCCGACCCAAGACAGCAGAGCCTGTAAAGTCCCCAACGTCTCCCACCCAGAACC TCTTCCCAGCCAGCAAAACCAGCCCTGTCAATCTGCCAAACAAAAGCAGCATCCCTAGCCCCATTGGAGGAAGCCTGGGAAGAGGATCCTCTTTAGATAT CCTATCTAGTCTCAACTCCCCTGCCCTTTTTGGGGATCAGGATACAGTGATGAAAGCCATTCAGGAGGCGCGGAAGATGCGAGAGCAGATCCAGCGGGAG CAACAGCAGCAACAGCCACATGGTGTTGACGGGAAACTGTCCTCCATAAATAATATGGGGCTGAACAGCTGCAGGAATGAAAAGGAAAGAACGCGCTTTG AGAATTTGGGGCCCCAGTTAACGGGAAAGTCAAATGAAGATGGAAAACTGGGCCCAGGTGTCATCGACCTTACTCGGCCAGAAGATGCAGAGGGAGGTGC CACTGTGGCTGAAGCACGAGTCTACAGGGACGCCCGCGGCCGTGCCAGCAGCGAGCCACACATTAAGCGACCAATGAATGCATTCATGGTTTGGGCAAAG GATGAGAGGAGAAAAATCCTTCAGGCCTTCCCCGACATGCATAACTCCAACATTAGCAAAATCTTAGGATCTCGCTGGAAATCAATGTCCAACCAGGAGA AGCAACCTTATTATGAAGAGCAGGCCCGGCTAAGCAAGATCCACTTAGAGAAGTACCCAAACTATAAATACAAACCCCGACCGAAACGCACCTGCATTGT TGATGGCAAAAAGCTTCGGATTGGGGAGTATAAGCAACTGATGAGGTCTCGGAGACAGGAGATGAGGCAGTTCTTTACTGTGGGGCAACAGCCTCAGATT CCAATCACCACAGGAACAGGTGTTGTGTATCCTGGTGCTATCACTATGGCAACTACCACACCATCGCCTCAGATGACATCTGACTGCTCTAGCACCTCGG CCAGCCCGGAGCCCAGCCTCCCGGTCATCCAGAGCACTTATGGTATGAAGACAGATGGCGGAAGCCTAGCTGGAAATGAAATGATCAATGGAGAGGATGA AATGGAAATGTATGATGACTATGAAGATGACCCCAAATCAGACTATAGCAGTGAAAATGAAGCCCCGGAGGCTGTCAGTGCCAACTGAGGAGTTTTTGTT TGCTGAATTAAAGTACTCTGACATTTCACCCCCCTCCCCAACAAAGAGTTATTAAAGAGCCCGCATGCATTTGTGGCTCCACAATTACATCAGCAGAATG GTCTTAATTGTTTCGTAAAGTGTGAGACAGATTAAGTTTTCCCTGATTTTTCATGAACTTGAGTTTTTTGTTGTTATTGTTATTGTTGTTGTTGTTGTTT TTTTAATTTAGGTGAAGACATATTAAATATGAGACACCAGGACTTGAAACTTATCTCAACCCGTAGATGTCTTACAAGTCTTATATTTTTGTCTTACTTT TTTTTTCTTTTGGATGTTGATAAAGGTTTAAGTTACTGTTTTAGATGGGGTTAAACATTCTCACTCAGGTATGCTGTGCCGGCCTACAGGTTGTGAATGT GTTTTTATTCTGAATTATTTTAGAAAACAACTGAGGATTTCATATTGTGAAACAGAACAAGTCCACGGCGTGTGCAGCTGCATGTAGAGCATATTCAAAA GGCCTCGGAATTCCAATTTTCCATTTGTAGAGTTAAACTTTGAATGTGCCAAACTTTTTCGTAACTTTTGAATCTTAATATTTTGAAAGTCTTAAAGGAG ACACTGCAAAGTCTTAGACAATCTTTGGCATCTTAAAATAAAATAGCAAACCAACATTTTTTTTTCCAGAAAATGGTAAGGTACTCAGGAATCTGGAGAC AAGATATTGTAAGGAATGAACAAGGTTGCCACAGTGCATGGACCCAATTGTGTTTGCCTGTTGACGTGCCATCAGTGCGTGATGTGGTATGACATACACA CACCAGAGCAACCGCCACACCAGATATCGACAGAGTGGTCTTCTCTGCCTGAGACCACCTCTCACTACATCCATTATCCCTTTGCCTTTAACCCTGACAT TCAGTCTTAACACATTTTATCTTAAATAATTTATTCATTCCAGAATGTCAAGGGTCCACTTGCTATTTATTTTTTTTCAATTGTTGGTGGCATTAATTTA ATAATTCTTGTTTTTCACCTTCCTTCCCCGAAGAACTTTTCCGTCCTTTTCACCTCCTTCTCCTGTGTACATAGTGATTTTATGTCCCCAGAACGCCTGG AAGCATTTCTGAAACCAAGATATTATTAAAAACCTATTATTGTTTTTAATCATGAGTATGTATCTGGCTGCAGGGCTGTGTATTGGGATATAGGTATATA GTCTTACACTTAAACAGGTATGCCCCTGAGGTTCACTGTGACCTCAAGTCTTTTGCCAGAATTTTCCCCTAATTCAGTTCACAAGTGGTAGGGTCTGCAT CAGTGGCATTTCCCCCTGAATTCCATTCAGCAGCAAGGTTCAACAGTGGTGACTGCCAGGCAGGAGAGTCCTGCGGCCAAACCTGAAGCCCAAGGCTCGT GGGCCATGCAGGAATCTCAGTGAAGCTGTCATGGGCTGGCACCTTTACACTGAGTTGCCTTGTCCCAGCTGGCACATCTAGGGAGTTCATTGCAAAATCC CCAGGATGCAAAAAGCCACATGACAGCCTCAGAGCAAAGATGGTGGCAAATAGTCATGATACATCTAGAGAATGAAAGAAAACTGTAAGGGAGGAGAAGG AGGGGAATACATTCCCTATATGGGATGTTCCTACTGTTAACTCTGGGGAACAGATAGCTCCGGGGGCAGCAGATGAGTTCCTCTGGCTGACTCTATCTGT AGCCACATGGGGACCTGCCTACGTGTGAACAAAATGAACTGCACTTATCACACAAGGATTTCTTTGAAGACATGCTACTGGGGTGGGAAGCAGTGAGGTT TTATTCCCCATCTCCTAACTACAGGGAGCTCTGCCATGTCATTTTGGCCTTCCTGAAACTAGGACAGGTTGTCTATCGGGGGGCTTCCCCCAGAGAGGTT TAGTGGGAGAATGTCAGTGAATGGGATAGTTCACCTCATGGGACAACCCAGAATCTGATCACCAGGACATAGGAATGGCCCCATCAGATTTCTTGAGCCA TTTTGTCACTTGGAAGAAAATAGTGTACCTTTGTATTTATTTAAGAGTGCTCAAGGCCTAATAGCAATAAACAGGTCTAGCCAAGAAATTACAAGCTATT CTGTTAGCTGGGAGTGCTCTCTATAAGCTGATTAAGGTACTGATAGGAACTCTTTGTTATTCATGTTGGTTGGGGATTAGAAATTTGTTTTTGTACATTT ATTTCAAATGAGGAGGAGGTCATTTTTTCTCTCAAAAAATGAGTATTTATTATTGTCTTACTGATTTCTTTGATTATATACCTCTCCTCCTCAGTTCACT CTTGTTTTTTTTCTTTCTCTTTGGCTTTTGCTTTTGCTCTCTCTCACTTCTTTCTTATTTTGTTGCATTGGTAGAGTGTTGTATGGCTAGCATTGTATTG TATGTAATTAATTTTGCACAAAAGCAAACATTTAGCATAGTAGGTTAATTTTGTTTGTTTTTATGACCATGCCAAAATAATATTCTGGGCTGGTGGAGAA CAAAGGACTGTTCTTTAGGACTGAAACTTGATTTTGCTCATAGTAAGT

refseq_SOX6.F4 (forward primer)
refseq_SOX6.R4 (reverse primer)
Single exon skipping

Frame 0

KGNLPVISGCWG*YFLCIFKNVFQASHLSICLC S*WRGCNDPGFNLKGKGRGQ*STCGLPSASAPHN AQQTSL*GATNTCQYHSTRC*LGQRSVISAKNG IRE**VMFPIFLPKYLYLTT*A*RRESGP*DND QCYFWNPRAPQRESCRCGGHTETEEA*GNDSD*T RGFLLHGKTTFKRLEGKNGKTKYQ*TSWRN*RY T*EPGRKRTAALHHDYPADQFTGAATGSA**TE KTGSVTN*ETTAANGPCSPTARTDCETTAATSAT AAQN*SPAATDPGSGSHASAHDPNFST*PADSG SSCCCPTGIPLPPWNNIQTR**LPRTVHSINNG SCCCFWTQPFTAPEGSCLPPTN*PKAKGPK*PFW QEFGHL*TWWWPLLQPQTD*AALCRSAGQHAGV TWSKDAINSTATKHSRDGLTYWDKK*KERDQPC NSS*G*SSSTASESLIPTQDSRACKVPNVSHPEP LPSQQNQPCQSAKQKQHP*PHWRKPGKRILFRY PI*SQLPCPFWGSGYSDESHSGGAEDARADPAG ATAATATWC*RETVLHK*YGAEQLQE*KGKNAL* EFGAPVNGKVK*RWKTGPRCHRPYSARRCRGRC HCG*STSLQGRPRPCQQRATH*ATNECIHGLGK G*EEKNPSGLPRHA*LQH*QNLRISLEINVQPGE ATLL*RAGPAKQDPLREVPKL*IQTPTETHLHC *WQKASDWGV*ATDEVSETGDEAVLYCGATASD SNHHRNRCCVSWCYHYGNYHTIASDDI*LL*HLG QPGAQPPGHPEHLWYEDRWRKPSWK*NDQWRG* NGNV**L*R*PQIRL*Q*K*SPGGCQCQLRSFC LLN*STLTFHPPPQQRVIKEPACICGSTITSAEW S*LFRKV*DRLSFP*FFMNLSFLLLLLLLLLLF F*FR*RHIKYETPGLETYLNP*MSYKSYIFVLL FFSFGC**RFKLLF*MGLNILTQVCCAGLQVVNV FLF*IILENN*GFHIVKQNKSTACAAACRAYSK GLGIPIFHL*S*TLNVPNFFVTFES*YFESLKG DTAKS*TIFGILK*NSKPTFFFPENGKVLRNLET RYCKE*TRLPQCMDPIVFAC*RAISA*CGMTYT HQSNRHTRYRQSGLLCLRPPLTTSIIPLPLTLT FSLNTFYLK*FIHSRMSRVHLLFIFFQLLVALI* *FLFFTFLPRRTFPSFSPPSPVYIVILCPQNAW KHF*NQDIIKNLLLFLIMSMYLAAGLCIGI*VY SLTLKQVCP*GSL*PQVFCQNFPLIQFTSGRVCI SGISP*IPFSSKVQQW*LPGRRVLRPNLKPKAR GPCRNLSEAVMGWHLYTELPCPSWHI*GVHCKI PRMQKAT*QPQSKDGGK*S*YI*RMKENCKGGEG GEYIPYMGCSYC*LWGTDSSGGSR*VPLADSIC SHMGTCLRVNKMNCTYHTRISLKTCYWGGKQ*G FIPHLLTTGSSAMSFWPS*N*DRLSIGGLPPERF SGRMSVNGIVHLMGQPRI*SPGHRNGPIRFLEP FCHLEENSVPLYLFKSAQGLIAINRSSQEITSY SVSWECSL*AD*GTDRNSLLFMLVGD*KFVFVHL FQMRRRSFFLSKNEYLLLSY*FL*LYTSPPQFT LVFFLSLWLLLLLSLTSFLFCCIGRVLYG*HCI VCN*FCTKANI*HSRLILFVFMTMPK*YSGLVEN KGLFFRTET*FCS**

Frame 1

KAIYQ*FLGAGADIFCAYLRMSSKQATSPFACA ADGEDAMTQDLTSREKEEGSDQHVASHLPLHPI MHNKPHSEELPTLVSTIQQDADWDSVLSSQQRME SENNKLCSLYSFRNTSTSPHKPDEGSRDREIMT SVTFGTPERRKGSLADVVDTLKQKKLEEMTRTE QEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGT PESLAEKERQLSTMITQLISLREQLLAAHDEQK KLAASQIEKQRQQMDLARQQQEQIARQQQQLLQ QQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLA AAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMA AAAASGLSPLQLQKGHVSHPQINQRLKGLSDRF GRNLDTFEHGGGHSYNHKQIEQLYAAQLASMQVS PGAKMPSTPQPPNTAGTVSPTGIKNEKRGTSPV TQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQN LFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDI LSSLNSPALFGDQDTVMKAIQEARKMREQIQRE QQQQQPHGVDGKLSSINNMGLNSCRNEKERTRF ENLGPQLTGKSNEDGKLGPGVIDLTRPEDAEGGA TVAEARVYRDARGRASSEPHIKRPMNAFMVWAK DERRKILQAFPDMHNSNISKILGSRWKSMSNQE KQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIV DGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQI PITTGTGVVYPGAITMATTTPSPQMTSDCSSTS ASPEPSLPVIQSTYGMKTDGGSLAGNEMINGEDE MEMYDDYEDDPKSDYSSENEAPEAVSAN*GVFV C*IKVL*HFTPLPNKELLKSPHAFVAPQLHQQN GLNCFVKCETD*VFPDFS*T*VFCCYCYCCCCCF FNLGEDILNMRHQDLKLISTRRCLTSLIFLSYF FFLLDVDKGLSYCFRWG*TFSLRYAVPAYRL*M CFYSELF*KTTEDFIL*NRTSPRRVQLHVEHIQK ASEFQFSICRVKL*MCQTFS*LLNLNILKVLKE TLQSLRQSLAS*NKIANQHFFFQKMVRYSGIWR QDIVRNEQGCHSAWTQLCLPVDVPSVRDVV*HTH TRATATPDIDRVVFSA*DHLSLHPLSLCL*P*H SVLTHFILNNLFIPECQGSTCYLFFFNCWWH*F NNSCFSPSFPEELFRPFHLLLLCT**FYVPRTPG SISETKILLKTYYCF*S*VCIWLQGCVLGYRYI VLHLNRYAPEVHCDLKSFARIFP*FSSQVVGSA SVAFPPEFHSAARFNSGDCQAGESCGQT*SPRLV GHAGISVKLSWAGTFTLSCLVPAGTSREFIAKS PGCKKPHDSLRAKMVANSHDTSRE*KKTVREEK EGNTFPIWDVPTVNSGEQIAPGAADEFLWLTLSV ATWGPAYV*TK*TALITQGFL*RHATGVGSSEV LFPIS*LQGALPCHFGLPETRTGCLSGGFPQRG LVGECQ*MG*FTSWDNPESDHQDIGMAPSDFLSH FVTWKKIVYLCIYLRVLKA**Q*TGLAKKLQAI LLAGSALYKLIKVLIGTLCYSCWLGIRNLFLYI YFK*GGGHFFSQKMSIYYCLTDFFDYIPLLLSSL LFFFFLFGFCFCSLSLLSYFVALVECCMASIVL YVINFAQKQTFSIVG*FCLFL*PCQNNILGWWR TKDCSLGLKLDFAHSK

Frame 2

RQFTSDFWVLGLIFFVHI*ECLPSKPPLHLPVQ LMERMQ*PRI*PQGKRKRAVINMWPPICLCTP* CTTNLTLRSYQHLSVPFNKMLTGTAFCHLSKEW NQRIISYVPYIPSEIPLPHHISLTKGVGTVR**P VLLLEPQSAAKGVLPMWWTH*NRRSLRK*LGLN KRIPPAWKNYFQKIGRKKWKD*IPVNFLEKLKV HLRAWQKKNGSSPP*LPS*SVYGSSYWQRMMNRK NWQRHKLRNNGSKWTLLANSKNRLRDNSSNFCN SSTKLISCSNRSRFRVTCLRS*SQFFHMTSGLW QQLLLPNRDSSSPLE*HTNQVITTPYSSFHQQWQ LLLLLDSALYSSRRVMSPTHKLTKG*RA*VTVL AGIWTPLNMVVATLTTTNRLSSSMPLSWPACRC HLEQRCHQLHSHQTQQGRSHLLG*KMKREGPAL* LKLRMKQQHSL*ISHPDPRQQSL*SPQRLPPRT SSQPAKPALSICQTKAASLAPLEEAWEEDPL*I SYLVSTPLPFLGIRIQ**KPFRRRGRCESRSSGS NSSNSHMVLTGNCPP*IIWG*TAAGMKRKERAL RIWGPS*RESQMKMENWAQVSSTLLGQKMQREV PLWLKHESTGTPAAVPAASHTLSDQ*MHSWFGQR MRGEKSFRPSPTCITPTLAKS*DLAGNQCPTRR SNLIMKSRPG*ARST*RSTQTINTNPDRNAPAL LMAKSFGLGSISN**GLGDRR*GSSLLWGNSLRF QSPQEQVLCILVLSLWQLPHHRLR*HLTALAPR PARSPASRSSRALMV*RQMAEA*LEMK*SMERM KWKCMMTMKMTPNQTIAVKMKPRRLSVPTEEFLF AELKYSDISPPSPTKSY*RARMHLWLHNYISRM VLIVS*SVRQIKFSLIFHELEFFVVIVIVVVVV FLI*VKTY*I*DTRT*NLSQPVDVLQVLYFCLTF FFFWMLIKV*VTVLDGVKHSHSGMLCRPTGCEC VFILNYFRKQLRISYCETEQVHGVCSCM*SIFK RPRNSNFPFVELNFECAKLFRNF*ILIF*KS*RR HCKVLDNLWHLKIK*QTNIFFSRKW*GTQESGD KIL*GMNKVATVHGPNCVCLLTCHQCVMWYDIH TPEQPPHQISTEWSSLPETTSHYIHYPFAFNPDI QS*HILS*IIYSFQNVKGPLAIYFFSIVGGINL IILVFHLPSPKNFSVLFTSFSCVHSDFMSPERL EAFLKPRYY*KPIIVFNHEYVSGCRAVYWDIGI* SYT*TGMPLRFTVTSSLLPEFSPNSVHKW*GLH QWHFPLNSIQQQGSTVVTARQESPAAKPEAQGS WAMQESQ*SCHGLAPLH*VALSQLAHLGSSLQNP QDAKSHMTASEQRWWQIVMIHLENERKL*GRRR RGIHSLYGMFLLLTLGNR*LRGQQMSSSG*LYL *PHGDLPTCEQNELHLSHKDFFEDMLLGWEAVRF YSPSPNYRELCHVILAFLKLGQVVYRGASPREV *WENVSEWDSSPHGTTQNLITRT*EWPHQIS*A ILSLGRK*CTFVFI*ECSRPNSNKQV*PRNYKLF C*LGVLSIS*LRY**ELFVIHVGWGLEICFCTF ISNEEEVIFSLKK*VFIIVLLISLIIYLSSSVH SCFFSFSLAFAFALSHFFLILLHW*SVVWLALYC M*LILHKSKHLA**VNFVCFYDHAKIIFWAGGE QRTVL*D*NLILLIVS