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Sequences

Sequence NM_014418

ACATGTCCTTAACCTTGGCACAACAAATCTCTAAATCGATTGAGACCTGTCTCAGATACTTCTTGGTTTACAAGATGGAGGCTGAAATTAAAGAGAAAGA TCCTCACTCAACTCACAGAGGAGCAGAATGGGAAGAGGACTTAGTGAGAATTCCCTGAACACAGGAGAAAACCGAAGCCCAGAGAGTGGATGGCCAAGTC CAAGGTCACACAGCCAAGTGCATCCGAGCAGCGCAGGATCCAGGTTTCCTGCCCCAGTGCTCTTTCTGCAGCCCATGCCACCTGTTTTCATGCTATCTCA GTTCACCTTCCAGCTACACACCTGCAATCAGAAACAGAAAGTCTGAGAGTGTGTGTGGAGCCAAAGAGGGGACAGCAGTGGTGAGGCCAGTTTCATGTCT TGTGATTTATTTTCTCCCTTTCTTTTTGGACTTTAATCTTAAGTGGACAGCAATTTGGAACAGTGTCAGCACCTTGTTGGATTGTGCTGTTTGGGAGAGG TGACACAGAGTATTTTAAAAGAGCAGGTTAAGAACTTTTCACAAGGAATCAATTGAGAGAAAATAAAATAGAAACAGACTGTCCCACATTTGGGCTGAGC ACCAAACACATTTTTTTTTTTTTTTTTGAGACTGAGTCTTGCTCTGTCACTCAGGCTGGAATGCAGTGGCGCGATCTCGGCTCACTGCAGCCTTCACCTC CCGGGTTCAAGTGATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCACCATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGA GGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCAAGTGATCCACCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAGGCACGAGCCACCGCA CCTGGCCTCAAACAAGCTTTTTAATTGCATTCAGAAGTGCACCAACTAGGCACAGACCTCTGGCATGGAGACAGAATGGGAGGTTCTTGGTGTCTGGCCT GGTCTTGCAGAAATCAGGGAGTTGTGGGGACCAGGCAGCCACTGTGCATCAGATAGGAGTTTCAGCAGCAGGCAACAGAAAGCCTGCCAATGTAGCTGAA ATAAGAAGGGCTTATTTTTATCCCTGAACAGAAGCTTCCAGCACTGGTTCATCAGATCAGGGCACTGAGCTGGCATCCCCATTTATACAGTTTTCCTTTC CTTCACCTTTGTAAGATGCCTGGCACAGCTCTGAGCATCAAATATCTGTGCCATGTGGGAGCACACGAGGGGACAAGTGGGGAATAGGGTCTCCTCTGTC CTCTGTCCCCAGGCTGGCTTCTCTCGTGGTAGAAGGACCCCCACAGCACCAGGCCTCATAACTACCCTGCTGGTAAACTGCAGGTGCTGTCACGGCTTCC TGCCTTCGTATATGAAATGTTCCTTGGCAGCTGGACTGCTGTTTGTTTACCGCACGCACAGCTCTGCTGCCTCTTGGTTACACCAGTGAGCTATTGCTCC ATGACAAGCCACACCGAAATGTAGCAAGCACTTATTGTTGCCCACATGTGTCAAGTCAGTTGAGTGTTTCTGCCAACCGGGGCCTGGCTTGGCCCACCTT GGCTGAGCTTGCTCTGGTGCTAGGGTCAGCTAGACAGGTCGGCAGGAGACCAGCTGCTCTAGAAGAGCCTGGACAGGATGAATTGGTTCTCCTTCCACGT GCATCTTGCATCCCTCCAGCCAAGGATTTGAACCCAGGTCTCGTCTGACTCCAAATGCACCAAGAGGATGGAGCCCAGAGTGACACAGAGGAAGAGGCCC CTGGATGGATGCATGGGGAAGATCACGGGAATTACAAGTGACATCCTGAAGTATGATCACAAATGTTTCAAATTAAGCCTTCCTGCTAAGTTTCCAGAGG TGTGTGGCTCGGACGAAGTGTTTCCAGACCCTGATCTACTGCATGTCCTGCCTGTTGCTGGTTCTTTGCAGCAGTCCATTGACCAATGCTGTCTACAGCT TGAGAGCCTCTGCAGGCCAGGGCTGCTCTGTGCACATCCTACGTTACTATTCAAACTGCACAGCAGCATGAAAAACAGGCCCTTCTTTTCTCTCATTTAC ACATATGTGAAGAAGACACAGCAAGTGAGAAAACGTGACCGAAAGCCACGCGGACAGGTGGCCGCAGGGCCAAACCCAACTTCCGTGATGTGATCTGGCT ACAGTTTGGAGCCCACATATTTCAATAGTTATCCCCAGGCCTGGCACGTCAAAGAGGCTCCAGAAATGTTTGCTGAGTGAGTGATGAATCAGCAATTTCT GATTTTCTCCTAAACTTAGAGAATAAGTGGCTTGTCTGCAGTCACCCAGGGCGTCCCTGACAGAGGTACAAGTAAAGCCCAGATCTCTGCATCTCTGGTT CAAGGCCTTTTGTACTACACACCGCTGTCTCTTTGTCATCATTAAAAGCAAGGCTCAAGGGACATGTTTTCTAGCCTTAGTTCCCCATCTACAAAATGGG CCTCATGGAATGGAATGTCTCCACTTCACTCCAGCATCAACAAGTGGGGAATTCTGATGGATTCAATTCGACTTCTTTCCATGGGCGTGTTCTAAGCAGC CTCTTTGTTCCAGAAGCTGCCCTCAGCCAGAGTTGGATAAGCCAATCCTCACTCCCCAGCCTCCTCTGGATAGGGATGAAGACCCCACTGGGGTTGGAAG TGCAGAGGCAGACAGGTGTATGGAGTCACCTGTAAATTGATTCAAGTGAGCCAGGAAAGCAGCAAAGGAAAGAGAAACCTGAGTGACGACGTGGTGGAGG AACAGGGCTGGAAAGAGGCTGCTGGCTGTCTGGCTTCGCAGCTCTGGCCTCCTAATCAGCCTCGCTCTTGTCTCTGGTGTTCTCTGGCTCTTGTCCATCT GTCTGTGTTTCTTTTTGCCAGCTATTGACTAATCTTTGCTGAAGCTGAGCTAGAATTCTGGTGTTTATAAGCAGGTAACTAGCTGAGCACTAGTTGATAA CTTTGGAGTTAGTGGATAACCACAGGGCTGGGCTACTAAGCTTTTTCAGTTGAAAAATAAATAAATAAATAAATAAACACCACACCCAGCTAATTTTTGT ATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGACTCCATCTCCTGACCTCGTGATCCGCCTACCTCGGCCTCCCAAAGTGCTGGGATTAC AGGCGTGAGCCACCACGCCCGGTCACCTTTGTCCTGTTTTCTACAGGTTAGAAGCAAGGCACAAGTCATACCTACATTCAAGGAGAGGGGACCATGCAAA GGATAAACTGCAGAAAGGACGTGGATGAAGCAGAGAGGCACGTGCCTGTCTCATTCGCCTCTGGATCTGCTGCATCCAGGACTGTGCCAGCACAAAGTGG GAGCCCGATAAATATTTATTGAAGAAATGAAAGGTGACAAAAGGCAGAAGGAGGGAGACCCCAAAAAGAGTTACACTGGAAGAAGAGCCCCTCCTTCCTT CAAGCCAAGTCATCCCCTTGGGCTCTCACCACTTCAGTTTATACCTTGCCTGTAGTCCAGTCTCTCCAGCATCAATTCCATCAGCACAAAGACATAATTT AATTCCTTCTACCTTAAAAAAAAAAAAAAAAAAAAAACAGCTCTCCCTCTCTTAGGGTGAGATGAAGTGGTTGGCAACAGGCCAATGCTACTTTCAGAGG CGTTTGAATCAGAGTGACTCCATTTTGAATAGGGGTTGAGTAAAATAAGGCTGAGACCTACTGGGCTGCATTCCCATGAGCTTAGGCATTCTAAGTCACA GGATGAGACAGGAGGTCAGCACAAGATACACGTCACAAAGACCTTGCTGATAAAACAGATTGTGATAAGAAAAGCCAGTCAAAACCCACCAAAACCAAGA TGGCGACAAAAGTGATCTCTGGTTGTCCTCACTGCTGATTATTCACTAATTATAAAATTAACATGATAAAAGACACTCCCACCAGCACCGTGACAGTTTA CAAATGACATGGACACATCAGGAAGTTACCCTATATGTCTAAAAGGGGAGGAGCCCTCCATTCCAATTGTCCACCCCTTTCCTGGAAAACTCATGAATAA TCCACCCCTTGTTTAGCATATAATCAAGAAATAACCATGAAAATAGCCAACCAGCAGCCCTCAGGGCTGCTTTGCCTATGGAGTAGCCATTCTATATTCC TTTACTTTCTTAATAAACTTGCTTTCACTTTA

refseq_TCL6.F1 (forward primer)
refseq_TCL6.R1 (reverse primer)
Single exon skipping

Frame 0

TCP*PWHNKSLNRLRPVSDTSWFTRWRLKLKRK ILTQLTEEQNGKRT**EFPEHRRKPKPREWMAKS KVTQPSASEQRRIQVSCPSALSAAHATCFHAIS VHLPATHLQSETESLRVCVEPKRGQQW*GQFHV L*FIFSLSFWTLILSGQQFGTVSAPCWIVLFGRG DTEYFKRAG*ELFTRNQLRENKIETDCPTFGLS TKHIFFFFFETESCSVTQAGMQWRDLGSLQPSP PGFK*FFCLSLPSSWDYRCPPPCQANFCIFSRDE VSPCWPGWS*TPDQVIHLLRPPKVLGLQARATA PGLKQAF*LHSEVHQLGTDLWHGDRMGGSWCLA WSCRNQGVVGTRQPLCIR*EFQQQATESLPM*LK *EGLIFIPEQKLPALVHQIRALSWHPHLYSFPF LHLCKMPGTALSIKYLCHVGAHEGTSGE*GLLC PLSPGWLLSW*KDPHSTRPHNYPAGKLQVLSRLP AFVYEMFLGSWTAVCLPHAQLCCLLVTPVSYCS MTSHTEM*QALIVAHMCQVS*VFLPTGAWLGPP WLSLLWC*GQLDRSAGDQLL*KSLDRMNWFSFHV HLASLQPRI*TQVSSDSKCTKRMEPRVTQRKRP LDGCMGKITGITSDILKYDHKCFKLSLPAKFPE VCGSDEVFPDPDLLHVLPVAGSLQQSIDQCCLQL ESLCRPGLLCAHPTLLFKLHSSMKNRPFFSLIY TYVKKTQQVRKRDRKPRGQVAAGPNPTSVM*SG YSLEPTYFNSYPQAWHVKEAPEMFAE*VMNQQFL IFS*T*RISGLSAVTQGVPDRGTSKAQISASLV QGLLYYTPLSLCHH*KQGSRDMFSSLSSPSTKW ASWNGMSPLHSSINKWGILMDSIRLLSMGVF*AA SLFQKLPSARVG*ANPHSPASSG*G*RPHWGWK CRGRQVYGVTCKLIQVSQESSKGKRNLSDDVVE EQGWKEAAGCLASQLWPPNQPRSCLWCSLALVHL SVFLFASY*LIFAEAELEFWCL*AGN*LSTS** LWS*WITTGLGY*AFSVEK*INK*INTTPS*FL YF**RQGFTVLARMDSIS*PRDPPTSASQSAGIT GVSHHARSPLSCFLQVRSKAQVIPTFKERGPCK G*TAERTWMKQRGTCLSHSPLDLLHPGLCQHKV GAR*IFIEEMKGDKRQKEGDPKKSYTGRRAPPSF KPSHPLGLSPLQFIPCL*SSLSSINSISTKT*F NSFYLKKKKKKKTALPLLG*DEVVGNRPMLLSE AFESE*LHFE*GLSKIRLRPTGLHSHELRHSKSQ DETGGQHKIHVTKTLLIKQIVIRKASQNPPKPR WRQK*SLVVLTADYSLIIKLT**KTLPPAP*QF TNDMDTSGSYPICLKGEEPSIPIVHPFPGKLMNN PPLV*HIIKK*P*K*PTSSPQGCFAYGVAILYS FTFLINLLSL

Frame 1

HVLNLGTTNL*ID*DLSQILLGLQDGG*N*RER SSLNSQRSRMGRGLSENSLNTGENRSPESGWPS PRSHSQVHPSSAGSRFPAPVLFLQPMPPVFMLSQ FTFQLHTCNQKQKV*ECVWSQRGDSSGEASFMS CDLFSPFLFGL*S*VDSNLEQCQHLVGLCCLGE VTQSILKEQVKNFSQGIN*EKIK*KQTVPHLG*A PNTFFFFFLRLSLALSLRLECSGAISAHCSLHL PGSSDSSASASQVAGITGAHHHARLIFVFLVET RFHHVGQAGLELLTK*STCFGLPKCWDYRHEPPH LASNKLFNCIQKCTN*AQTSGMETEWEVLGVWP GLAEIRELWGPGSHCASDRSFSSRQQKACQCS* NKKGLFLSLNRSFQHWFIRSGH*AGIPIYTVFLS FTFVRCLAQL*ASNICAMWEHTRGQVGNRVSSV LCPQAGFSRGRRTPTAPGLITTLLVNCRCCHGF LPSYMKCSLAAGLLFVYRTHSSAASWLHQ*AIAP *QATPKCSKHLLLPTCVKSVECFCQPGPGLAHL G*ACSGARVS*TGRQETSCSRRAWTG*IGSPST CILHPSSQGFEPRSRLTPNAPRGWSPE*HRGRGP WMDAWGRSRELQVTS*SMITNVSN*AFLLSFQR CVARTKCFQTLIYCMSCLLLVLCSSPLTNAVYS LRASAGQGCSVHILRYYSNCTAA*KTGPSFLSFT HM*RRHSK*ENVTESHADRWPQGQTQLP*CDLA TVWSPHISIVIPRPGTSKRLQKCLLSE**ISNF *FSPKLRE*VACLQSPRASLTEVQVKPRSLHLWF KAFCTTHRCLFVIIKSKAQGTCFLALVPHLQNG PHGMECLHFTPASTSGEF*WIQFDFFPWACSKQ PLCSRSCPQPELDKPILTPQPPLDRDEDPTGVGS AEADRCMESPVN*FK*ARKAAKERET*VTTWWR NRAGKRLLAVWLRSSGLLISLALVSGVLWLLSI CLCFFLPAID*SLLKLS*NSGVYKQVTS*ALVDN FGVSG*PQGWATKLFQLKNK*INK*TPHPANFC IFSRDRVSPC*PGWTPSPDLVIRLPRPPKVLGL QA*ATTPGHLCPVFYRLEARHKSYLHSRRGDHAK DKLQKGRG*SREARACLIRLWICCIQDCASTKW EPDKYLLKK*KVTKGRRRETPKRVTLEEEPLLP SSQVIPLGSHHFSLYLACSPVSPASIPSAQRHNL IPSTLKKKKKKKQLSLS*GEMKWLATGQCYFQR RLNQSDSILNRG*VK*G*DLLGCIPMSLGILSH RMRQEVSTRYTSQRPC**NRL**EKPVKTHQNQD GDKSDLWLSSLLIIH*L*N*HDKRHSHQHRDSL QMTWTHQEVTLYV*KGRSPPFQLSTPFLENS*I IHPLFSI*SRNNHENSQPAALRAALPME*PFYIP LLS**TCFHF

Frame 2

MSLTLAQQISKSIETCLRYFLVYKMEAEIKEKD PHSTHRGAEWEEDLVRIP*TQEKTEAQRVDGQV QGHTAKCIRAAQDPGFLPQCSFCSPCHLFSCYL SSPSSYTPAIRNRKSESVCGAKEGTAVVRPVSCL VIYFLPFFLDFNLKWTAIWNSVSTLLDCAVWER *HRVF*KSRLRTFHKESIERK*NRNRLSHIWAE HQTHFFFFF*D*VLLCHSGWNAVARSRLTAAFTS RVQVILLPQPPK*LGLQVPTTMPG*FLYF**RR GFTMLARLVLNS*PSDPPASASQSAGITGTSHR TWPQTSFLIAFRSAPTRHRPLAWRQNGRFLVSGL VLQKSGSCGDQAATVHQIGVSAAGNRKPANVAE IRRAYFYP*TEASSTGSSDQGTELASPFIQFSF PSPL*DAWHSSEHQISVPCGSTRGDKWGIGSPLS SVPRLASLVVEGPPQHQAS*LPCW*TAGAVTAS CLRI*NVPWQLDCCLFTARTALLPLGYTSELLL HDKPHRNVASTYCCPHVSSQLSVSANRGLAWPTL AELALVLGSARQVGRRPAALEEPGQDELVLLPR ASCIPPAKDLNPGLV*LQMHQEDGAQSDTEEEA PGWMHGEDHGNYK*HPEV*SQMFQIKPSC*VSRG VWLGRSVSRP*STACPACCWFFAAVH*PMLSTA *EPLQARAALCTSYVTIQTAQQHEKQALLFSHL HICEEDTASEKT*PKATRTGGRRAKPNFRDVIWL QFGAHIFQ*LSPGLARQRGSRNVC*VSDESAIS DFLLNLENKWLVCSHPGRP*QRYK*SPDLCISG SRPFVLHTAVSLSSLKARLKGHVF*P*FPIYKMG LMEWNVSTSLQHQQVGNSDGFNSTSFHGRVLSS LFVPEAALSQSWISQSSLPSLLWIGMKTPLGLE VQRQTGVWSHL*IDSSEPGKQQRKEKPE*RRGGG TGLERGCWLSGFAALAS*SASLLSLVFSGSCPS VCVSFCQLLTNLC*S*ARILVFISR*LAEH*LI TLELVDNHRAGLLSFFS*KINK*INKHHTQLIFV FLVETGFHRVSQDGLHLLTS*SAYLGLPKCWDY RREPPRPVTFVLFSTG*KQGTSHTYIQGEGTMQ RINCRKDVDEAERHVPVSFASGSAASRTVPAQSG SPINIY*RNER*QKAEGGRPQKELHWKKSPSFL QAKSSPWALTTSVYTLPVVQSLQHQFHQHKDII *FLLP*KKKKKKNSSPSLRVR*SGWQQANATFRG V*IRVTPF*IGVE*NKAETYWAAFP*A*AF*VT G*DRRSAQDTRHKDLADKTDCDKKSQSKPTKTK MATKVISGCPHC*LFTNYKINMIKDTPTSTVTVY K*HGHIRKLPYMSKRGGALHSNCPPLSWKTHE* STPCLAYNQEITMKIANQQPSGLLCLWSSHSIF LYFLNKLAFTL

Sequence NM_012468

ACATGTCCTTAACCTTGGCACAACAAATCTCTAAATCGATTGAGACCTGTCTCAGATACTTCTTGGTTTACAAGATGGAGGCTGAAATTAAAGAGAAAGA TCCTCACTCAACTCACAGAGGAGCAGAATGGGAAGAGGACTTAGTGAGAATTCCCTGAACACAGGAGAAAACCGAAGCCCAGAGAGTGGATGGCCAAGTC CAAGGTCACACAGCCAAGTGCATCCGAGCAGCGCAGGATCCAGGTTTCCTGCCCCAGTGCTCTTTCTGCAGCCCATGCCACCTGTTTTCATGCTATCTCA GTTCACCTTCCAGCTACACACCTGCAATCAGAAACAGAAAGTCTGAGAGTGTGTGTGGAGCCAAAGAGGGGACAGCAGTGGTGAGGCCAGTTTCATGTCT TGTGATTTATTTTCTCCCTTTCTTTTTGGACTTTAATCTTAAGTGGACAGCAATTTGGAACAGTGTCAGCACCTTGTTGGATTGTGCTGTTTGGGAGAGG TGACACAGAGTATTTTAAAAGAGCAGGTTAAGAACTTTTCACAAGGAATCAATTGAGAGAAAATAAAATAGAAACAGACTGTCCCACATTTGGGCTGAGC ACCAAACACATTTTTTTTTTTTTTTTTGAGACTGAGTCTTGCTCTGTCACTCAGGCTGGAATGCAGTGGCGCGATCTCGGCTCACTGCAGCCTTCACCTC CCGGGTTCAAGTGATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCACCATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGA GGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCAAGTGATCCACCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAGGCACGAGCCACCGCA CCTGGCCTCAAACAAGCTTTTTAATTGCATTCAGAAGTGCACCAACTAGGCACAGACCTCTGGCATGGAGACAGAATGGGAGGTTCTTGGTGTCTGGCCT GGTCTTGCAGAAATCAGGGAGTTGTGGGGACCAGGCAGCCACTGTGCATCAGATAGGAGTTTCAGCAGCAGGCAACAGAAAGCCTGCCAATGTAGCTGAA ATAAGAAGGGCTTATTTTTATCCCTGAACAGAAGCTTCCAGCACTGGTTCATCAGATCAGGGCACTGAGCTGGCATCCCCATTTATACAGTTTTCCTTTC CTTCACCTTTGTAAGATGCCTGGCACAGCTCTGAGCATCAAATATCTGTGCCATGTGGGAGCACACGAGGGGACAAGTGGGGAATAGGGTCTCCTCTGTC CTCTGTCCCCAGGCTGGCTTCTCTCGTGGTAGAAGGACCCCCACAGCACCAGGCCTCATAACTACCCTGCTGGTAAACTGCAGGTGCTGTCACGGCTTCC TGCCTTCGTATATGAAATGTTCCTTGGCAGCTGGACTGCTGTTTGTTTACCGCACGCACAGCTCTGCTGCCTCTTGGTTACACCAGTGAGCTATTGCTCC ATGACAAGCCACACCGAAATGTAGCAAGCACTTATTGTTGCCCACATGTGTCAAGTCAGTTGAGTGTTTCTGCCAACCGGGGCCTGGCTTGGCCCACCTT GGCTGAGCTTGCTCTGGTGCTAGGGTCAGCTAGACAGGTCGGCAGGAGACCAGCTGCTCTAGAAGAGCCTGGACAGGATGAATTGGTTCTCCTTCCACGT GCATCTTGCATCCCTCCAGCCAAGGATTTGAACCCAGGTCTCGTCTGACTCCAAATGCACCAAGAGGATGGAGCCCAGAGTGACACAGAGGAAGAGGCCC CTGGATGGATGCATGGGGAAGATCACGGGAATTACAAGTGACATCCTGAAGTATGATCACAAATGTTTCAAATTAAGCCTTCCTGCTAAGTTTCCAGAGG TGTGTGGCTCGGACGAAGTGTTTCCAGACCCTGATCTACTGCATGTCCTGCCTGTTGCTGGTTCTTTGCAGCAGTCCATTGACCAATGCTGTCTACAGCT TGAGAGCCTCTGCAGGCCAGGGCTGCTCTGTGCACATCCTACGTTACTATTCAAACTGCACAGCAGCATGAAAAACAGGCCCTTCTTTTCTCTCATTTAC ACATATGTGAAGAAGACACAGCAAGTGAGAAAACGTGACCGAAAGCCACGCGGACAGGTGGCCGCAGGGCCAAACCCAACTTCCGTGATGTGATCTGGCT ACAGTTTGGAGCCCACATATTTCAATAGTTATCCCCAGGCCTGGCACGTCAAAGAGGCTCCAGAAATGTTTGCTGAGTGAGTGATGAATCAGCAATTTCT GATTTTCTCCTAAACTTAGAGAATAAGTGGCTTGTCTGCAGTCACCCAGGGCGTCCCTGACAGAGGTACAAGTAAAGCCCAGATCTCTGCATCTCTGGTT CAAGGCCTTTTGTACTACACACCGCTGTCTCTTTGTCATCATTAAAAGCAAGGCTCAAGGGACATGTTTTCTAGCCTTAGTTCCCCATCTACAAAATGGG CCTCATGGAATGGAATGTCTCCACTTCACTCCAGCATCAACAAGTGGGGAATTCTGATGGATTCAATTCGACTTCTTTCCATGGGCGTGTTCTAAGCAGC CTCTTTGTTCCAGAAGCTGCCCTCAGCCAGAGTTGGATAAGCCAATCCTCACTCCCCAGCCTCCTCTGGATAGGGATGAAGACCCCACTGGGGTTGGAAG TGCAGAGGCAGACAGGTGTATGGAGTCACCTGTAAATTGATTCAAGTGAGCCAGGAAAGCAGCAAAGGAAAGAGAAACCTGAGTGACGACGTGGTGGAGG AACAGGGCTGGAAAGAGGCTGCTGGCTGTCTGGCTTCGCAGCTCTGGCCTCCTAATCAGCCTCGCTCTTGTCTCTGGTGTTCTCTGGCTCTTGTCCATCT GTCTGTGTTTCTTTTTGCCAGCTATTGACTAATCTTTGCTGAAGCTGAGCTAGAATTCTGGTGTTTATAAGCAGGTAACTAGCTGAGCACTAGTTGATAA CTTTGGAGTTAGTGGATAACCACAGGGCTGGGCTACTAAGCTTTTTCAGTTGAAAAATAAATAAATAAATAAATAAACACCACACCCAGCTAATTTTTGT ATTTTTAGTAGAGACAGGGTTTCACCGTGTTAGCCAGGATGGACTCCATCTCCTGACCTCGTGATCCGCCTACCTCGGCCTCCCAAAGTGCTGGGATTAC AGGCGTGAGCCACCACGCCCGGTCACCTTTGTCCTGTTTTCTACAGGTTAGAAGCAAGGCACAAGTCATACCTACATTCAAGGAGAGGGGACCATGCAAA GGATAAACTGCAGAGAGACAACAGATTTACATCCCAGACACATTCACACTGGGCCAAGAGCAGCTGCCATTGCAGATTCGAGTCACGTCCTTTTTTCCTT CCTTCTCCTTCTTGGAGTTACAACAGAAGCTGGGGAGGTGAGAGTGCAGAAAGGACGTGGATGAAGCAGAGAGGCACGTGCCTGTCTCATTCGCCTCTGG ATCTGCTGCATCCAGGACTGTGCCAGCACAAAGTGGGAGCCCGATAAATATTTATTGAAGAAATGAAAGGTGACAAAAGGCAGAAGGAGGGAGACCCCAA AAAGAGCAGCACAGGAAGCTGCCCCCTCAAACACTAAAGGCTCCTGGAGCTATTCACAGTGCTGGGGAACAACCAGAGGAGGAGGAGGACAGAGGGAGAG AATACTTTTTTTAAAAATCCCAAACAAAACCAAATTCTATACATGTATATCTCGAGTGAGTAAATGTGTAACACTCAGCA

refseq_TCL6.F1 (forward primer)
refseq_TCL6.R1 (reverse primer)
Single exon skipping

Frame 0

TCP*PWHNKSLNRLRPVSDTSWFTRWRLKLKRK ILTQLTEEQNGKRT**EFPEHRRKPKPREWMAKS KVTQPSASEQRRIQVSCPSALSAAHATCFHAIS VHLPATHLQSETESLRVCVEPKRGQQW*GQFHV L*FIFSLSFWTLILSGQQFGTVSAPCWIVLFGRG DTEYFKRAG*ELFTRNQLRENKIETDCPTFGLS TKHIFFFFFETESCSVTQAGMQWRDLGSLQPSP PGFK*FFCLSLPSSWDYRCPPPCQANFCIFSRDE VSPCWPGWS*TPDQVIHLLRPPKVLGLQARATA PGLKQAF*LHSEVHQLGTDLWHGDRMGGSWCLA WSCRNQGVVGTRQPLCIR*EFQQQATESLPM*LK *EGLIFIPEQKLPALVHQIRALSWHPHLYSFPF LHLCKMPGTALSIKYLCHVGAHEGTSGE*GLLC PLSPGWLLSW*KDPHSTRPHNYPAGKLQVLSRLP AFVYEMFLGSWTAVCLPHAQLCCLLVTPVSYCS MTSHTEM*QALIVAHMCQVS*VFLPTGAWLGPP WLSLLWC*GQLDRSAGDQLL*KSLDRMNWFSFHV HLASLQPRI*TQVSSDSKCTKRMEPRVTQRKRP LDGCMGKITGITSDILKYDHKCFKLSLPAKFPE VCGSDEVFPDPDLLHVLPVAGSLQQSIDQCCLQL ESLCRPGLLCAHPTLLFKLHSSMKNRPFFSLIY TYVKKTQQVRKRDRKPRGQVAAGPNPTSVM*SG YSLEPTYFNSYPQAWHVKEAPEMFAE*VMNQQFL IFS*T*RISGLSAVTQGVPDRGTSKAQISASLV QGLLYYTPLSLCHH*KQGSRDMFSSLSSPSTKW ASWNGMSPLHSSINKWGILMDSIRLLSMGVF*AA SLFQKLPSARVG*ANPHSPASSG*G*RPHWGWK CRGRQVYGVTCKLIQVSQESSKGKRNLSDDVVE EQGWKEAAGCLASQLWPPNQPRSCLWCSLALVHL SVFLFASY*LIFAEAELEFWCL*AGN*LSTS** LWS*WITTGLGY*AFSVEK*INK*INTTPS*FL YF**RQGFTVLARMDSIS*PRDPPTSASQSAGIT GVSHHARSPLSCFLQVRSKAQVIPTFKERGPCK G*TAERQQIYIPDTFTLGQEQLPLQIRVTSFFP SFSFLELQQKLGR*ECRKDVDEAERHVPVSFASG SAASRTVPAQSGSPINIY*RNER*QKAEGGRPQ KEQHRKLPPQTLKAPGAIHSAGEQPEEEEDRGR EYFF*KSQTKPNSIHVYLE*VNV*HSA

Frame 1

HVLNLGTTNL*ID*DLSQILLGLQDGG*N*RER SSLNSQRSRMGRGLSENSLNTGENRSPESGWPS PRSHSQVHPSSAGSRFPAPVLFLQPMPPVFMLSQ FTFQLHTCNQKQKV*ECVWSQRGDSSGEASFMS CDLFSPFLFGL*S*VDSNLEQCQHLVGLCCLGE VTQSILKEQVKNFSQGIN*EKIK*KQTVPHLG*A PNTFFFFFLRLSLALSLRLECSGAISAHCSLHL PGSSDSSASASQVAGITGAHHHARLIFVFLVET RFHHVGQAGLELLTK*STCFGLPKCWDYRHEPPH LASNKLFNCIQKCTN*AQTSGMETEWEVLGVWP GLAEIRELWGPGSHCASDRSFSSRQQKACQCS* NKKGLFLSLNRSFQHWFIRSGH*AGIPIYTVFLS FTFVRCLAQL*ASNICAMWEHTRGQVGNRVSSV LCPQAGFSRGRRTPTAPGLITTLLVNCRCCHGF LPSYMKCSLAAGLLFVYRTHSSAASWLHQ*AIAP *QATPKCSKHLLLPTCVKSVECFCQPGPGLAHL G*ACSGARVS*TGRQETSCSRRAWTG*IGSPST CILHPSSQGFEPRSRLTPNAPRGWSPE*HRGRGP WMDAWGRSRELQVTS*SMITNVSN*AFLLSFQR CVARTKCFQTLIYCMSCLLLVLCSSPLTNAVYS LRASAGQGCSVHILRYYSNCTAA*KTGPSFLSFT HM*RRHSK*ENVTESHADRWPQGQTQLP*CDLA TVWSPHISIVIPRPGTSKRLQKCLLSE**ISNF *FSPKLRE*VACLQSPRASLTEVQVKPRSLHLWF KAFCTTHRCLFVIIKSKAQGTCFLALVPHLQNG PHGMECLHFTPASTSGEF*WIQFDFFPWACSKQ PLCSRSCPQPELDKPILTPQPPLDRDEDPTGVGS AEADRCMESPVN*FK*ARKAAKERET*VTTWWR NRAGKRLLAVWLRSSGLLISLALVSGVLWLLSI CLCFFLPAID*SLLKLS*NSGVYKQVTS*ALVDN FGVSG*PQGWATKLFQLKNK*INK*TPHPANFC IFSRDRVSPC*PGWTPSPDLVIRLPRPPKVLGL QA*ATTPGHLCPVFYRLEARHKSYLHSRRGDHAK DKLQRDNRFTSQTHSHWAKSSCHCRFESRPFFL PSPSWSYNRSWGGESAERTWMKQRGTCLSHSPL DLLHPGLCQHKVGAR*IFIEEMKGDKRQKEGDPK KSSTGSCPLKH*RLLELFTVLGNNQRRRRTEGE NTFFKNPKQNQILYMYISSE*MCNTQ

Frame 2

MSLTLAQQISKSIETCLRYFLVYKMEAEIKEKD PHSTHRGAEWEEDLVRIP*TQEKTEAQRVDGQV QGHTAKCIRAAQDPGFLPQCSFCSPCHLFSCYL SSPSSYTPAIRNRKSESVCGAKEGTAVVRPVSCL VIYFLPFFLDFNLKWTAIWNSVSTLLDCAVWER *HRVF*KSRLRTFHKESIERK*NRNRLSHIWAE HQTHFFFFF*D*VLLCHSGWNAVARSRLTAAFTS RVQVILLPQPPK*LGLQVPTTMPG*FLYF**RR GFTMLARLVLNS*PSDPPASASQSAGITGTSHR TWPQTSFLIAFRSAPTRHRPLAWRQNGRFLVSGL VLQKSGSCGDQAATVHQIGVSAAGNRKPANVAE IRRAYFYP*TEASSTGSSDQGTELASPFIQFSF PSPL*DAWHSSEHQISVPCGSTRGDKWGIGSPLS SVPRLASLVVEGPPQHQAS*LPCW*TAGAVTAS CLRI*NVPWQLDCCLFTARTALLPLGYTSELLL HDKPHRNVASTYCCPHVSSQLSVSANRGLAWPTL AELALVLGSARQVGRRPAALEEPGQDELVLLPR ASCIPPAKDLNPGLV*LQMHQEDGAQSDTEEEA PGWMHGEDHGNYK*HPEV*SQMFQIKPSC*VSRG VWLGRSVSRP*STACPACCWFFAAVH*PMLSTA *EPLQARAALCTSYVTIQTAQQHEKQALLFSHL HICEEDTASEKT*PKATRTGGRRAKPNFRDVIWL QFGAHIFQ*LSPGLARQRGSRNVC*VSDESAIS DFLLNLENKWLVCSHPGRP*QRYK*SPDLCISG SRPFVLHTAVSLSSLKARLKGHVF*P*FPIYKMG LMEWNVSTSLQHQQVGNSDGFNSTSFHGRVLSS LFVPEAALSQSWISQSSLPSLLWIGMKTPLGLE VQRQTGVWSHL*IDSSEPGKQQRKEKPE*RRGGG TGLERGCWLSGFAALAS*SASLLSLVFSGSCPS VCVSFCQLLTNLC*S*ARILVFISR*LAEH*LI TLELVDNHRAGLLSFFS*KINK*INKHHTQLIFV FLVETGFHRVSQDGLHLLTS*SAYLGLPKCWDY RREPPRPVTFVLFSTG*KQGTSHTYIQGEGTMQ RINCRETTDLHPRHIHTGPRAAAIADSSHVLFSF LLLLGVTTEAGEVRVQKGRG*SREARACLIRLW ICCIQDCASTKWEPDKYLLKK*KVTKGRRRETP KRAAQEAAPSNTKGSWSYSQCWGTTRGGGGQRER ILFLKIPNKTKFYTCISRVSKCVTLS

Sequence NM_020553

GTCAACAGCCACTTCCTCCCTGGGGCTTAACAGGCATTCCTTGAAGCCTGCCCCGGGCAGTTAGTCCTTCCCTTTTGTGTGAACTCTCGGCTGTTTTCGT TGTCTGCACACAGAGCAGTGGTCTAGGGAGCGAAGGGTTTTGGAATGGACCTTTTCCTTTCTTAGCTTGCTGTAGGGATTGTGCTTCAGCTAAGTAAAGT GGAAAAACAGAAGTACAAAAGCAACAGCCCCAGATCTAAGGGCACCGTAGCTCTGGAATCTGGATTTTATTCCAGATTCAACCCTCAGCAGGGAGTTGAA GTGGTCAGCCCTGTGCTTTACAAGGCTGATTGGGCAGCACCCATAGTAGAATTGTCAATATAAAGTAATTGAAAGGATCAGAATCCAGGGTTTTCGTTGT TTTTAAGTTTTTAAGTTTTAAAAACAAAGTTGTTTTTGTTCGTTTTTAAGTTTACTTCTCTTTAATCGGTCTCACTTTATTGACCAGGCTGGTTTCGAAC TCCTGGGCTTAAGTGATTTCTCCTGTCTCAGCTTCTCAAAGTGCTGGGATTACAGGCGTGAGCCGCTGCACCCAGCCCAGAATCCAGTTTTAAAGAGTTT ATTCAAGCCAAAAGCTGGGAATGGCCATCTGAGAAACACAGACTCTAGGAAAATGGGGCCAGGGCCCTGAAGTTAAAAGTTAAAGTCTTGCTTACATAGG CAGAAAGCAAAGACATTTAGTAACCTTGTAGAACATTTTCTTGTATAACTTTTCTTGTTTTAACTTTCTTGTTTAACATTTTCTATGCAAGGCTGGTTTA TGAGTTACAACCTATAATTAATTATAGGTTGTTCCCCTGCCTCCACCTTGTTTTCTTTTCTCTACAGCTTGTTTTCATTTCCTTTCCAATTTAAGAATGT GTTTAACATTCTATCCCAGACAATGTGATGGTCATGAAGTCTCTGTCAGAGAGGAAAGAGGGAGTTAATCTATAATGAAGATCAACAGTGAAGAGGAAAG GGGTCTTCCCTGGAACGCTTCAGTCATATACGACATTTTACACAACAATAATGGACCTGTGTAAGGAGGTATGTGTGGGAGAAGATTCCAGAAGGTCTTG AGACACTCCAGATGAAGGTTGGTGTGGGGCTGTGCTGCTGGAGCACCAGAGAGTGAAAAGAGGTTATCTCCCTCTGTGCCAGGCCAGAGTGCAGTGGTGC AATCACGGCTCACTGTAGTCTCAACCTCCTGGGCTCAAGAGATCCTCCCACTTCAGCCTCCCAAGCAGCTAAGACTACAGTTATTTTATTCTTATGAGCT TGTGGGCTCCATCTACCAACCTCTGGGCAAATGCTCATCTGACTTCCATAGACACACAGCAAGCAGACAACCACACAAAGAGGAAGAGGGAGAATATTTA CCAGCCATGGAACATCTGCTCTGTGCTTATATCTCATATGCATTATCTCTAATTATCAAAAGAAGGTTAGAAGCAAGGCACAAGTCATACCTACATTCAA GGAGAGGGGACCATGCAAAGGATAAACTGCAGAGAGACAACAGATTTACATCCCAGACACATTCACACTGGGCCAAGAGCAGCTGCCATTGCAGATTCGA GTCACGTCCTTTTTTCCTTCCTTCTCCTTCTTGGAGTTACAACAGAAGCTGGGGAGGTGAGAGTGCAGAAAGGACGTGGATGAAGCAGAGAGGCACGTGC CTGTCTCATTCGCCTCTGGATCTGCTGCATCCAGGACTGTGCCAGCACAAAGTGGGAGCCCGATAAATATTTATTGAAGAAATGAAAGGTGACAAAAGGC AGAAGGAGGGAGACCCCAAAAAGAGAGATGGGATCCCACTATATTGCACAGGCTGGTCTCAAACTCCTGAGCTCAAGCGATCCTCCTGCCTTGGCCTCCC ACGGTGCTGAGACTACAGGTGTGAGCCACCACTCCCAGCCTGATTTATATTTTATAAAGAGGCAACGATGGAGTACACAGCTGAGCAATCTCTTGCATTT CTGGACCTTGAGCATCAGGAAGCTTAGGGAGGGTGAAAACTCAGAAGAAAGCTCAGAGTTGAGACCTTTGATTTTTTGCCCAGAGAGTCCAGCTAACACA CTTTATAACCCAAAAGGCCTTACAAATATTTCAGAGCAGAGGCAGCATTTTTGCACTGGATAAACAAAGGTACGGTAATTTCTTCATAGTTTATTTTCAT TACAAGTAATTCTTTTCCTGAAGAAAAATTTATATTTCAGATTTATTTCCCCTCTCAAAGGGGAGGGGAGTTATAATATTCTGTTTCCATAGCACCATTC CTCAAGATCAAACCCAGGCAGGCATTTTTACAGCACACACTTCCATATGTCATTGCTAATCCTCGCAACCTTGAGGAAACTGAGGTGCAAAGGGGTTGAG GAATGTGTTCACCATCCCAAAGCCAACGAAGTGCCAGAACCCAAATCCAAGCTTGGCTGTCTCATCCCCAAGGACGTACTCTTTATGCCGTTCCTTATGA CTTTTCATGCAGCAGGTGCAAGGCAGCAAGAATTTGGCTCTTCTGACATTTATCAGGCACCTGGTATGTGCCAGGCCTACTGTAAACCCCAAGATGACAT GGTCCATGTCTATCCATTCAGCATCAGAGAGACTGTTATCAGGACTTGAGGCCTGAGACACAGCCCTGAATCAGATGCAGGCCTCTGGTGTTTGCAAAGG ATGCGTGAGTATGTGTATGTGTATGCGTGCATTGTGTGTAGGGGTGTGTGTATGTTGGGGGTTGGGGGCAGAAAATGCTCCCTGAACTGTTGGTATTAAG GCAGATCTTTGATGGAGGGACTGGGGTGTGGAGACTTGAACCCTAGTTTCAAAGTGGGGACCTCGAACACTAGTATGGCCAGCAGACAGGCTGGAGTTGC TGAATTGTCAGCTAGGTCTTTAATCCACTCCCCTTCTCTAAATCAGGGTGGAATTGGACTCAGGGCTGGTTCAGGGCTGAGGTTTCCGGGGGATGAATGA TTCCAGACAGTGGGAAGGGGTGATGGATGATGAACCTGGCAAGTCACAGGACTATGGACTTGGCTGTATGTTGCATGTATGTGTAAGATCTGAGTTGAGA CCTTTGATTTTTGCCCAGAAAGTCCAGCTAACATACTATATAAGCCAGTGAGACCAGCCAGTGTCTGTTCATTGCCTCTGCCTTGCCCCTATGCCTGCCC GCCTCCTATAGCAGGTCCCTATAGCATAATGAAAATGCGAACAACCTTTTCTCTGGACACTGGGGAATCTAGTCTGGTGGATAAAGATGGATGGCTGTTA AACGATC

refseq_TCL6.F1 (forward primer)
refseq_TCL6.R1 (reverse primer)
Single exon skipping

Frame 0

VNSHFLPGA*QAFLEACPGQLVLPFCVNSRLFS LSAHRAVV*GAKGFGMDLFLS*LAVGIVLQLSKV EKQKYKSNSPRSKGTVALESGFYSRFNPQQGVE VVSPVLYKADWAAPIVELSI*SN*KDQNPGFSL FLSF*VLKTKLFLFVFKFTSL*SVSLY*PGWFRT PGLK*FLLSQLLKVLGLQA*AAAPSPESSFKEF IQAKSWEWPSEKHRL*ENGARALKLKVKVLLT* AESKDI**PCRTFSCITFLVLTFLFNIFYARLVY ELQPIINYRLFPCLHLVFFSLQLVFISFPI*EC V*HSIPDNVMVMKSLSERKEGVNL**RSTVKRK GVFPGTLQSYTTFYTTIMDLCKEVCVGEDSRRS* DTPDEGWCGAVLLEHQRVKRGYLPLCQARVQWC NHGSL*SQPPGLKRSSHFSLPSS*DYSYFILMS LWAPSTNLWANAHLTSIDTQQADNHTKRKRENIY QPWNICSVLISHMHYL*LSKEG*KQGTSHTYIQ GEGTMQRINCRETTDLHPRHIHTGPRAAAIADS SHVLFSFLLLLGVTTEAGEVRVQKGRG*SREARA CLIRLWICCIQDCASTKWEPDKYLLKK*KVTKG RRRETPKREMGSHYIAQAGLKLLSSSDPPALAS HGAETTGVSHHSQPDLYFIKRQRWSTQLSNLLHF WTLSIRKLREGENSEESSELRPLIFCPESPANT LYNPKGLTNISEQRQHFCTG*TKVR*FLHSLFS LQVILFLKKNLYFRFISPLKGEGSYNILFP*HHS SRSNPGRHFYSTHFHMSLLILATLRKLRCKGVE ECVHHPKANEVPEPKSKLGCLIPKDVLFMPFLM TFHAAGARQQEFGSSDIYQAPGMCQAYCKPQDDM VHVYPFSIRETVIRT*GLRHSPESDAGLWCLQR MREYVYVYACIVCRGVCMLGVGGRKCSLNCWY* GRSLMEGLGCGDLNPSFKVGTSNTSMASRQAGVA ELSARSLIHSPSLNQGGIGLRAGSGLRFPGDE* FQTVGRGDG**TWQVTGLWTWLYVACMCKI*VE TFDFCPESPANILYKPVRPASVCSLPLPCPYACP PPIAGPYSIMKMRTTFSLDTGESSLVDKDGWLL ND

Frame 1

STATSSLGLNRHSLKPAPGS*SFPFV*TLGCFR CLHTEQWSRERRVLEWTFSFLSLL*GLCFS*VK WKNRSTKATAPDLRAP*LWNLDFIPDSTLSRELK WSALCFTRLIGQHP**NCQYKVIERIRIQGFRC F*VFKF*KQSCFCSFLSLLLFNRSHFIDQAGFE LLGLSDFSCLSFSKCWDYRREPLHPAQNPVLKSL FKPKAGNGHLRNTDSRKMGPGP*S*KLKSCLHR QKAKTFSNLVEHFLV*LFLF*LSCLTFSMQGWF MSYNL*LIIGCSPASTLFSFLYSLFSFPFQFKNV FNILSQTM*WS*SLCQRGKRELIYNEDQQ*RGK GSSLERFSHIRHFTQQ*WTCVRRYVWEKIPEGL ETLQMKVGVGLCCWSTRE*KEVISLCARPECSGA ITAHCSLNLLGSRDPPTSASQAAKTTVILFL*A CGLHLPTSGQMLI*LP*THSKQTTTQRGRGRIF TSHGTSALCLYLICIISNYQKKVRSKAQVIPTFK ERGPCKG*TAERQQIYIPDTFTLGQEQLPLQIR VTSFFPSFSFLELQQKLGR*ECRKDVDEAERHV PVSFASGSAASRTVPAQSGSPINIY*RNER*QKA EGGRPQKERWDPTILHRLVSNS*AQAILLPWPP TVLRLQV*ATTPSLIYIL*RGNDGVHS*AISCI SGP*ASGSLGRVKTQKKAQS*DL*FFAQRVQLTH FITQKALQIFQSRGSIFALDKQRYGNFFIVYFH YK*FFS*RKIYISDLFPLSKGRGVIIFCFHSTI PQDQTQAGIFTAHTSICHC*SSQP*GN*GAKGLR NVFTIPKPTKCQNPNPSLAVSSPRTYSLCRSL* LFMQQVQGSKNLALLTFIRHLVCARPTVNPKMT WSMSIHSASERLLSGLEA*DTALNQMQASGVCKG CVSMCMCMRALCVGVCVCWGLGAENAP*TVGIK ADL*WRDWGVET*TLVSKWGPRTLVWPADRLEL LNCQLGL*STPLL*IRVELDSGLVQG*GFRGMND SRQWEGVMDDEPGKSQDYGLGCMLHVCVRSELR PLIFAQKVQLTYYISQ*DQPVSVHCLCLAPMPA RLL*QVPIA**KCEQPFLWTLGNLVWWIKMDGC* TI

Frame 2

QQPLPPWGLTGIP*SLPRAVSPSLLCELSAVFV VCTQSSGLGSEGFWNGPFPFLACCRDCASAK*S GKTEVQKQQPQI*GHRSSGIWILFQIQPSAGS* SGQPCALQG*LGSTHSRIVNIK*LKGSESRVFVV FKFLSFKNKVVFVRF*VYFSLIGLTLLTRLVSN SWA*VISPVSASQSAGITGVSRCTQPRIQF*RV YSSQKLGMAI*ETQTLGKWGQGPEVKS*SLAYIG RKQRHLVTL*NIFLYNFSCFNFLV*HFLCKAGL *VTTYN*L*VVPLPPPCFLFSTACFHFLSNLRM CLTFYPRQCDGHEVSVREERGS*SIMKINSEEER GLPWNASVIYDILHNNNGPV*GGMCGRRFQKVL RHSR*RLVWGCAAGAPESEKRLSPSVPGQSAVV QSRLTVVSTSWAQEILPLQPPKQLRLQLFYSYEL VGSIYQPLGKCSSDFHRHTASRQPHKEEEGEYL PAMEHLLCAYISYALSLIIKRRLEARHKSYLHS RRGDHAKDKLQRDNRFTSQTHSHWAKSSCHCRFE SRPFFLPSPSWSYNRSWGGESAERTWMKQRGTC LSHSPLDLLHPGLCQHKVGAR*IFIEEMKGDKR QKEGDPKKRDGIPLYCTGWSQTPELKRSSCLGLP RC*DYRCEPPLPA*FIFYKEATMEYTAEQSLAF LDLEHQEA*GG*KLRRKLRVETFDFLPRESS*H TL*PKRPYKYFRAEAAFLHWINKGTVISS*FIFI TSNSFPEEKFIFQIYFPSQRGGEL*YSVSIAPF LKIKPRQAFLQHTLPYVIANPRNLEETEVQRG* GMCSPSQSQRSARTQIQAWLSHPQGRTLYAVPYD FSCSRCKAARIWLF*HLSGTWYVPGLL*TPR*H GPCLSIQHQRDCYQDLRPETQP*IRCRPLVFAK DA*VCVCVCVHCV*GCVYVGGWGQKMLPELLVLR QIFDGGTGVWRLEP*FQSGDLEH*YGQQTGWSC *IVS*VFNPLPFSKSGWNWTQGWFRAEVSGG*M IPDSGKG*WMMNLASHRTMDLAVCCMYV*DLS*D L*FLPRKSS*HTI*ASETSQCLFIASALPLCLP ASYSRSL*HNENANNLFSGHWGI*SGG*RWMAV KR

Sequence NM_020550

ACATGTCCTTAACCTTGGCACAACAAATCTCTAAATCGATTGAGACCTGTCTCAGATACTTCTTGGTTTACAAGATGGAGGCTGAAATTAAAGAGAAAGA TCCTCACTCAACTCACAGAGGAGCAGAATGGGAAGAGGACTTAGTGAGAATTCCCTGAACACAGGAGAAAACCGAAGCCCAGAGAGTGGATGGCCAAGTC CAAGGTCACACAGCCAAGTGCATCCGAGCAGCGCAGGATCCAGGTTTCCTGCCCCAGTGCTCTTTCTGCAGCCCATGCCACCTGTTTTCATGCTATCTCA GTTCACCTTCCAGCTACACACCTGCAATCAGAAACAGAAAGTCTGAGAGTGTGTGTGGAGCCAAAGAGGGGACAGCAGTGGTGAGGCCAGTTTCATGTCT TGTGATTTATTTTCTCCCTTTCTTTTTGGACTTTAATCTTAAGTGGACAGCAATTTGGAACAGTGTCAGCACCTTGTTGGATTGTGCTGTTTGGGAGAGG TGACACAGAGTATTTTAAAAGAGCAGGTTAAGAACTTTTCACAAGGAATCAATTGAGAGAAAATAAAATAGAAACAGACTGTCCCACATTTGGGCTGAGC ACCAAACACATTTTTTTTTTTTTTTTTGAGACTGAGTCTTGCTCTGTCACTCAGGCTGGAATGCAGTGGCGCGATCTCGGCTCACTGCAGCCTTCACCTC CCGGGTTCAAGTGATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCACCATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGA GGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCAAGTGATCCACCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAGGCACGAGCCACCGCA CCTGGCCTCAAACAAGCTTTTTAATTGCATTCAGAAGTGCACCAACTAGGCACAGACCTCTGGCATGGAGACAGAATGGGAGGTTCTTGGTGTCTGGCCT GGTCTTGCAGAAATCAGGGAGTTGTGGGGACCAGGCAGCCACTGTGCATCAGATAGGAGTTTCAGCAGCAGGCAACAGAAAGCCTGCCAATGTAGCTGAA ATAAGAAGGGCTTATTTTTATCCCTGAACAGAAGCTTCCAGCACTGGTTCATCAGATCAGGGCACTGAGCTGGCATCCCCATTTATACAGTTTTCCTTTC CTTCACCTTTGTAAGATGCCTGGCACAGCTCTGAGCATCAAATATCTGTGCCATGTGGGAGCACACGAGGGGACAAGTGGGGAATAGGGTCTCCTCTGTC CTCTGTCCCCAGGCTGGCTTCTCTCGTGGTAGAAGGACCCCCACAGCACCAGGCCTCATAACTACCCTGCTGGTAAACTGCAGGTGCTGTCACGGCTTCC TGCCTTCGTATATGAAATGTTCCTTGGCAGCTGGACTGCTGTTTGTTTACCGCACGCACAGCTCTGCTGCCTCTTGGTTACACCAGTGAGCTATTGCTCC ATGACAAGCCACACCGAAATGTAGCAAGCACTTATTGTTGCCCACATGTGTCAAGTCAGTTGAGTGTTTCTGCCAACCGGGGCCTGGCTTGGCCCACCTT GGCTGAGCTTGCTCTGGTGCTAGGGTCAGCTAGACAGGTCGGCAGGAGACCAGCTGCTCTAGAAGAGCCTGGACAGGATGAATTGGTTCTCCTTCCACGT GCATCTTGCATCCCTCCAGCCAAGGATTTGAACCCAGGTCTCGTCTGACTCCAAATGCACCAAGAGGATGGAGCCCAGAGTGACACAGAGGAAGAGGCCC CTGGATGGATGCATGGGGAAGATCACGGGAATTACAAGTGACATCCTGAAGTATGATCACAAATGTTTCAAATTAAGCCTTCCTGCTAAGTTTCCAGAGG TGTGTGGCTCGGACGAAGTGTTTCCAGACCCTGATCTACTGCATGTCCTGCCTGTTGCTGGTTCTTTGCAGCAGTCCATTGACCAATGCTGTCTACAGCT TGAGAGCCTCTGCAGGCCAGGGCTGCTCTGTGCACATCCTACGTTACTATTCAAACTGCACAGCAGCATGAAAAACAGGCCCTTCTTTTCTCTCATTTAC ACATATGTGAAGAAGACACAGCAAGTGAGAAAACGTGACCGAAAGCCACGCGGACAGGTGGCCGCAGGGCCAAACCCAACTTCCGTGATGTGATCTGGCT ACAGTTTGGAGCCCACATATTTCAATAGTTATCCCCAGGCCTGGCACGTCAAAGAGGCTCCAGAAATGTTTGCTGAGTTAGAAGCAAGGCACAAGTCATA CCTACATTCAAGGAGAGGGGACCATGCAAAGGATAAACTGCAGAGAGACAACAGATTTACATCCCAGACACATTCACACTGGGCCAAGAGCAGCTGCCAT TGCAGATTCGAGTCACGTCCTTTTTTCCTTCCTTCTCCTTCTTGGAGTTACAACAGAAGCTGGGGAGGTGAGAGTGCAGAAAGGACGTGGATGAAGCAGA GAGGCACGTGCCTGTCTCATTCGCCTCTGGATCTGCTGCATCCAGGACTGTGCCAGCACAAAGTGGGAGCCCGATAAATATTTATTGAAGAAATGAAAGG TGACAAAAGGCAGAAGGAGGGAGACCCCAAAAAGAGTTACACTGGAAGAAGAGCCCCTCCTTCCTTCAAGCCAAGTCATCCCCTTGGGCTCTCACCACTT CAGTTTATACCTTGCCTGTAGTCCAGTCTCTCCAGCATCAATTCCATCAGCACAAAGACATAATTTAATTCCTTCTACCTTAAAAAAAAAAAAAAAAAAA AAACAGCTCTCCCTCTCTTAGGGTGAGATGAAGTGGTTGGCAACAGGCCAATGCTACTTTCAGAGGCGTTTGAATCAGAGTGACTCCATTTTGAATAGGG GTTGAGTAAAATAAGGCTGAGACCTACTGGGCTGCATTCCCATGAGCTTAGGCATTCTAAGTCACAGGATGAGACAGGAGGTCAGCACAAGATACACGTC ACAAAGACCTTGCTGATAAAACAGATTGTGATAAGAAAAGCCAGTCAAAACCCACCAAAACCAAGATGGCGACAAAAGTGATCTCTGGTTGTCCTCACTG CTGATTATTCACTAATTATAAAATTAACATGATAAAAGACACTCCCACCAGCACCGTGACAGTTTACAAATGACATGGACACATCAGGAAGTTACCCTAT ATGTCTAAAAGGGGAGGAGCCCTCCATTCCAATTGTCCACCCCTTTCCTGGAAAACTCATGAATAATCCACCCCTTGTTTAGCATATAATCAAGAAATAA CCATGAAAATAGCCAACCAGCAGCCCTCAGGGCTGCTTTGCCTATGGAGTAGCCATTCTATATTCCTTTACTTTCTTAATAAACTTGCTTTCACTTTA

refseq_TCL6.F1 (forward primer)
refseq_TCL6.R1 (reverse primer)
Single exon skipping

Frame 0

TCP*PWHNKSLNRLRPVSDTSWFTRWRLKLKRK ILTQLTEEQNGKRT**EFPEHRRKPKPREWMAKS KVTQPSASEQRRIQVSCPSALSAAHATCFHAIS VHLPATHLQSETESLRVCVEPKRGQQW*GQFHV L*FIFSLSFWTLILSGQQFGTVSAPCWIVLFGRG DTEYFKRAG*ELFTRNQLRENKIETDCPTFGLS TKHIFFFFFETESCSVTQAGMQWRDLGSLQPSP PGFK*FFCLSLPSSWDYRCPPPCQANFCIFSRDE VSPCWPGWS*TPDQVIHLLRPPKVLGLQARATA PGLKQAF*LHSEVHQLGTDLWHGDRMGGSWCLA WSCRNQGVVGTRQPLCIR*EFQQQATESLPM*LK *EGLIFIPEQKLPALVHQIRALSWHPHLYSFPF LHLCKMPGTALSIKYLCHVGAHEGTSGE*GLLC PLSPGWLLSW*KDPHSTRPHNYPAGKLQVLSRLP AFVYEMFLGSWTAVCLPHAQLCCLLVTPVSYCS MTSHTEM*QALIVAHMCQVS*VFLPTGAWLGPP WLSLLWC*GQLDRSAGDQLL*KSLDRMNWFSFHV HLASLQPRI*TQVSSDSKCTKRMEPRVTQRKRP LDGCMGKITGITSDILKYDHKCFKLSLPAKFPE VCGSDEVFPDPDLLHVLPVAGSLQQSIDQCCLQL ESLCRPGLLCAHPTLLFKLHSSMKNRPFFSLIY TYVKKTQQVRKRDRKPRGQVAAGPNPTSVM*SG YSLEPTYFNSYPQAWHVKEAPEMFAELEARHKSY LHSRRGDHAKDKLQRDNRFTSQTHSHWAKSSCH CRFESRPFFLPSPSWSYNRSWGGESAERTWMKQ RGTCLSHSPLDLLHPGLCQHKVGAR*IFIEEMKG DKRQKEGDPKKSYTGRRAPPSFKPSHPLGLSPL QFIPCL*SSLSSINSISTKT*FNSFYLKKKKKK KTALPLLG*DEVVGNRPMLLSEAFESE*LHFE*G LSKIRLRPTGLHSHELRHSKSQDETGGQHKIHV TKTLLIKQIVIRKASQNPPKPRWRQK*SLVVLT ADYSLIIKLT**KTLPPAP*QFTNDMDTSGSYPI CLKGEEPSIPIVHPFPGKLMNNPPLV*HIIKK* P*K*PTSSPQGCFAYGVAILYSFTFLINLLSL

Frame 1

HVLNLGTTNL*ID*DLSQILLGLQDGG*N*RER SSLNSQRSRMGRGLSENSLNTGENRSPESGWPS PRSHSQVHPSSAGSRFPAPVLFLQPMPPVFMLSQ FTFQLHTCNQKQKV*ECVWSQRGDSSGEASFMS CDLFSPFLFGL*S*VDSNLEQCQHLVGLCCLGE VTQSILKEQVKNFSQGIN*EKIK*KQTVPHLG*A PNTFFFFFLRLSLALSLRLECSGAISAHCSLHL PGSSDSSASASQVAGITGAHHHARLIFVFLVET RFHHVGQAGLELLTK*STCFGLPKCWDYRHEPPH LASNKLFNCIQKCTN*AQTSGMETEWEVLGVWP GLAEIRELWGPGSHCASDRSFSSRQQKACQCS* NKKGLFLSLNRSFQHWFIRSGH*AGIPIYTVFLS FTFVRCLAQL*ASNICAMWEHTRGQVGNRVSSV LCPQAGFSRGRRTPTAPGLITTLLVNCRCCHGF LPSYMKCSLAAGLLFVYRTHSSAASWLHQ*AIAP *QATPKCSKHLLLPTCVKSVECFCQPGPGLAHL G*ACSGARVS*TGRQETSCSRRAWTG*IGSPST CILHPSSQGFEPRSRLTPNAPRGWSPE*HRGRGP WMDAWGRSRELQVTS*SMITNVSN*AFLLSFQR CVARTKCFQTLIYCMSCLLLVLCSSPLTNAVYS LRASAGQGCSVHILRYYSNCTAA*KTGPSFLSFT HM*RRHSK*ENVTESHADRWPQGQTQLP*CDLA TVWSPHISIVIPRPGTSKRLQKCLLS*KQGTSH TYIQGEGTMQRINCRETTDLHPRHIHTGPRAAAI ADSSHVLFSFLLLLGVTTEAGEVRVQKGRG*SR EARACLIRLWICCIQDCASTKWEPDKYLLKK*K VTKGRRRETPKRVTLEEEPLLPSSQVIPLGSHHF SLYLACSPVSPASIPSAQRHNLIPSTLKKKKKK KQLSLS*GEMKWLATGQCYFQRRLNQSDSILNR G*VK*G*DLLGCIPMSLGILSHRMRQEVSTRYTS QRPC**NRL**EKPVKTHQNQDGDKSDLWLSSL LIIH*L*N*HDKRHSHQHRDSLQMTWTHQEVTL YV*KGRSPPFQLSTPFLENS*IIHPLFSI*SRNN HENSQPAALRAALPME*PFYIPLLS**TCFHF

Frame 2

MSLTLAQQISKSIETCLRYFLVYKMEAEIKEKD PHSTHRGAEWEEDLVRIP*TQEKTEAQRVDGQV QGHTAKCIRAAQDPGFLPQCSFCSPCHLFSCYL SSPSSYTPAIRNRKSESVCGAKEGTAVVRPVSCL VIYFLPFFLDFNLKWTAIWNSVSTLLDCAVWER *HRVF*KSRLRTFHKESIERK*NRNRLSHIWAE HQTHFFFFF*D*VLLCHSGWNAVARSRLTAAFTS RVQVILLPQPPK*LGLQVPTTMPG*FLYF**RR GFTMLARLVLNS*PSDPPASASQSAGITGTSHR TWPQTSFLIAFRSAPTRHRPLAWRQNGRFLVSGL VLQKSGSCGDQAATVHQIGVSAAGNRKPANVAE IRRAYFYP*TEASSTGSSDQGTELASPFIQFSF PSPL*DAWHSSEHQISVPCGSTRGDKWGIGSPLS SVPRLASLVVEGPPQHQAS*LPCW*TAGAVTAS CLRI*NVPWQLDCCLFTARTALLPLGYTSELLL HDKPHRNVASTYCCPHVSSQLSVSANRGLAWPTL AELALVLGSARQVGRRPAALEEPGQDELVLLPR ASCIPPAKDLNPGLV*LQMHQEDGAQSDTEEEA PGWMHGEDHGNYK*HPEV*SQMFQIKPSC*VSRG VWLGRSVSRP*STACPACCWFFAAVH*PMLSTA *EPLQARAALCTSYVTIQTAQQHEKQALLFSHL HICEEDTASEKT*PKATRTGGRRAKPNFRDVIWL QFGAHIFQ*LSPGLARQRGSRNVC*VRSKAQVI PTFKERGPCKG*TAERQQIYIPDTFTLGQEQLP LQIRVTSFFPSFSFLELQQKLGR*ECRKDVDEAE RHVPVSFASGSAASRTVPAQSGSPINIY*RNER *QKAEGGRPQKELHWKKSPSFLQAKSSPWALTT SVYTLPVVQSLQHQFHQHKDII*FLLP*KKKKKK NSSPSLRVR*SGWQQANATFRGV*IRVTPF*IG VE*NKAETYWAAFP*A*AF*VTG*DRRSAQDTR HKDLADKTDCDKKSQSKPTKTKMATKVISGCPHC *LFTNYKINMIKDTPTSTVTVYK*HGHIRKLPY MSKRGGALHSNCPPLSWKTHE*STPCLAYNQEI TMKIANQQPSGLLCLWSSHSIFLYFLNKLAFTL

Sequence NM_020552

ACATGTCCTTAACCTTGGCACAACAAATCTCTAAATCGATTGAGACCTGTCTCAGATACTTCTTGGTTTACAAGATGGAGGCTGAAATTAAAGAGAAAGA TCCTCACTCAACTCACAGAGGAGCAGAATGGGAAGAGGACTTAGTGAGAATTCCCTGAACACAGGAGAAAACCGAAGCCCAGAGAGTGGATGGCCAAGTC CAAGGTCACACAGCCAAGTGCATCCGAGCAGCGCAGGATCCAGGTTTCCTGCCCCAGTGCTCTTTCTGCAGCCCATGCCACCTGTTTTCATGCTATCTCA GTTCACCTTCCAGCTACACACCTGCAATCAGAAACAGAAAGTCTGAGAGTGTGTGTGGAGCCAAAGAGGGGACAGCAGTGGTGAGGCCAGTTTCATGTCT TGTGATTTATTTTCTCCCTTTCTTTTTGGACTTTAATCTTAAGTGGACAGCAATTTGGAACAGTGTCAGCACCTTGTTGGATTGTGCTGTTTGGGAGAGG TGACACAGAGTATTTTAAAAGAGCAGGTTAAGAACTTTTCACAAGGAATCAATTGAGAGAAAATAAAATAGAAACAGACTGTCCCACATTTGGGCTGAGC ACCAAACACATTTTTTTTTTTTTTTTTGAGACTGAGTCTTGCTCTGTCACTCAGGCTGGAATGCAGTGGCGCGATCTCGGCTCACTGCAGCCTTCACCTC CCGGGTTCAAGTGATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCACCACCATGCCAGGCTAATTTTTGTATTTTTAGTAGAGACGA GGTTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCAAGTGATCCACCTGCTTCGGCCTCCCAAAGTGCTGGGATTACAGGCACGAGCCACCGCA CCTGGCCTCAAACAAGCTTTTTAATTGCATTCAGAAGTGCACCAACTAGGCACAGACCTCTGGCATGGAGACAGAATGGGAGGTTCTTGGTGTCTGGCCT GGTCTTGCAGAAATCAGGGAGTTGTGGGGACCAGGCAGCCACTGTGCATCAGATAGGAGTTTCAGCAGCAGGCAACAGAAAGCCTGCCAATGTAGCTGAA ATAAGAAGGGCTTATTTTTATCCCTGAACAGAAGCTTCCAGCACTGGTTCATCAGATCAGGGCACTGAGCTGGCATCCCCATTTATACAGTTTTCCTTTC CTTCACCTTTGTAAGATGCCTGGCACAGCTCTGAGCATCAAATATCTGTGCCATGTGGGAGCACACGAGGGGACAAGTGGGGAATAGGGTCTCCTCTGTC CTCTGTCCCCAGGCTGGCTTCTCTCGTGGTAGAAGGACCCCCACAGCACCAGGCCTCATAACTACCCTGCTGGTAAACTGCAGGTGCTGTCACGGCTTCC TGCCTTCGTATATGAAATGTTCCTTGGCAGCTGGACTGCTGTTTGTTTACCGCACGCACAGCTCTGCTGCCTCTTGGTTACACCAGTGAGCTATTGCTCC ATGACAAGCCACACCGAAATGTAGCAAGCACTTATTGTTGCCCACATGTGTCAAGTCAGTTGAGTGTTTCTGCCAACCGGGGCCTGGCTTGGCCCACCTT GGCTGAGCTTGCTCTGGTGCTAGGGTCAGCTAGACAGGTCGGCAGGAGACCAGCTGCTCTAGAAGAGCCTGGACAGGATGAATTGGTTCTCCTTCCACGT GCATCTTGCATCCCTCCAGCCAAGGATTTGAACCCAGGTCTCGTCTGACTCCAAATGCACCAAGAGGATGGAGCCCAGAGTGACACAGAGGAAGAGGCCC CTGGATGGATGCATGGGGAAGGTTAGAAGCAAGGCACAAGTCATACCTACATTCAAGGAGAGGGGACCATGCAAAGGATAAACTGCAGAGAGACAACAGA TTTACATCCCAGACACATTCACACTGGGCCAAGAGCAGCTGCCATTGCAGATTCGAGTCACGTCCTTTTTTCCTTCCTTCTCCTTCTTGGAGTTACAACA GAAGCTGGGGAGGTGAGAGTGCAGAAAGGACGTGGATGAAGCAGAGAGGCACGTGCCTGTCTCATTCGCCTCTGGATCTGCTGCATCCAGGACTGTGCCA GCACAAAGTGGGAGCCCGATAAATATTTATTGAAGAAATGAAAGGTGACAAAAGGCAGAAGGAGGGAGACCCCAAAAAGAGAGATGGGATCCCACTATAT TGCACAGGCTGGTCTCAAACTCCTGAGCTCAAGCGATCCTCCTGCCTTGGCCTCCCACGGTGCTGAGACTACAGGTGTGAGCCACCACTCCCAGCCTGAT TTATATTTTATAAAGAGGCAACGATGGAGTACACAGCTGAGCAATCTCTTGCATTTCTGGACCTTGAGCATCAGGAAGCTTAGGGAGGGTGAAAACTCAG AAGAAAGCTCAGAGTTGAGACCTTTGATTTTTTGCCCAGAGAGTCCAGCTAACACACTTTATAACCCAAAAGGCCTTACAAATATTTCAGAGCAGAGGCA GCATTTTTGCACTGGATAAACAAAGGTACGGTAATTTCTTCATAGTTTATTTTCATTACAAGTAATTCTTTTCCTGAAGAAAAATTTATATTTCAGATTT ATTTCCCCTCTCAAAGGGGAGGGGAGTTATAATATTCTGTTTCCATAGCACCATTCCTCAAGATCAAACCCAGGCAGGCATTTTTACAGCACACACTTCC ATATGTCATTGCTAATCCTCGCAACCTTGAGGAAACTGAGGTGCAAAGGGGTTGAGGAATGTGTTCACCATCCCAAAGCCAACGAAGTGCCAGAACCCAA ATCCAAGCTTGGCTGTCTCATCCCCAAGGACGTACTCTTTATGCCGTTCCTTATGACTTTTCATGCAGCAGGTGCAAGGCAGCAAGAATTTGGCTCTTCT GACATTTATCAGGCACCTGGTATGTGCCAGGCCTACTGTAAACCCCAAGATGACATGGTCCATGTCTATCCATTCAGCATCAGAGAGACTGTTATCAGGA CTTGAGGCCTGAGACACAGCCCTGAATCAGATGCAGGCCTCTGGTGTTTGCAAAGGATGCGTGAGTATGTGTATGTGTATGCGTGCATTGTGTGTAGGGG TGTGTGTATGTTGGGGGTTGGGGGCAGAAAATGCTCCCTGAACTGTTGGTATTAAGGCAGATCTTTGATGGAGGGACTGGGGTGTGGAGACTTGAACCCT AGTTTCAAAGTGGGGACCTCGAACACTAGTATGGCCAGCAGACAGGCTGGAGTTGCTGAATTGTCAGCTAGGTCTTTAATCCACTCCCCTTCTCTAAATC AGGGTGGAATTGGACTCAGGGCTGGTTCAGGGCTGAGGTTTCCGGGGGATGAATGATTCCAGACAGTGGGAAGGGGTGATGGATGATGAACCTGGCAAGT CACAGGACTATGGACTTGGCTGTATGTTGCATGTATGTGTAAGATCTGAGTTGAGACCTTTGATTTTTGCCCAGAAAGTCCAGCTAACATACTATATAAG CCAGTGAGACCAGCCAGTGTCTGTTCATTGCCTCTGCCTTGCCCCTATGCCTGCCCGCCTCCTATAGCAGGTCCCTATAGCATAATGAAAATGCGAACAA CCTTTTCTCTGGACACTGGGGAATCTAGTCTGGTGGATAAAGATGGATGGCTGTTAAACGATC

refseq_TCL6.F1 (forward primer)
refseq_TCL6.R1 (reverse primer)
Single exon skipping

Frame 0

TCP*PWHNKSLNRLRPVSDTSWFTRWRLKLKRK ILTQLTEEQNGKRT**EFPEHRRKPKPREWMAKS KVTQPSASEQRRIQVSCPSALSAAHATCFHAIS VHLPATHLQSETESLRVCVEPKRGQQW*GQFHV L*FIFSLSFWTLILSGQQFGTVSAPCWIVLFGRG DTEYFKRAG*ELFTRNQLRENKIETDCPTFGLS TKHIFFFFFETESCSVTQAGMQWRDLGSLQPSP PGFK*FFCLSLPSSWDYRCPPPCQANFCIFSRDE VSPCWPGWS*TPDQVIHLLRPPKVLGLQARATA PGLKQAF*LHSEVHQLGTDLWHGDRMGGSWCLA WSCRNQGVVGTRQPLCIR*EFQQQATESLPM*LK *EGLIFIPEQKLPALVHQIRALSWHPHLYSFPF LHLCKMPGTALSIKYLCHVGAHEGTSGE*GLLC PLSPGWLLSW*KDPHSTRPHNYPAGKLQVLSRLP AFVYEMFLGSWTAVCLPHAQLCCLLVTPVSYCS MTSHTEM*QALIVAHMCQVS*VFLPTGAWLGPP WLSLLWC*GQLDRSAGDQLL*KSLDRMNWFSFHV HLASLQPRI*TQVSSDSKCTKRMEPRVTQRKRP LDGCMGKVRSKAQVIPTFKERGPCKG*TAERQQ IYIPDTFTLGQEQLPLQIRVTSFFPSFSFLELQQ KLGR*ECRKDVDEAERHVPVSFASGSAASRTVP AQSGSPINIY*RNER*QKAEGGRPQKERWDPTI LHRLVSNS*AQAILLPWPPTVLRLQV*ATTPSLI YIL*RGNDGVHS*AISCISGP*ASGSLGRVKTQ KKAQS*DL*FFAQRVQLTHFITQKALQIFQSRG SIFALDKQRYGNFFIVYFHYK*FFS*RKIYISDL FPLSKGRGVIIFCFHSTIPQDQTQAGIFTAHTS ICHC*SSQP*GN*GAKGLRNVFTIPKPTKCQNP NPSLAVSSPRTYSLCRSL*LFMQQVQGSKNLALL TFIRHLVCARPTVNPKMTWSMSIHSASERLLSG LEA*DTALNQMQASGVCKGCVSMCMCMRALCVG VCVCWGLGAENAP*TVGIKADL*WRDWGVET*TL VSKWGPRTLVWPADRLELLNCQLGL*STPLL*I RVELDSGLVQG*GFRGMNDSRQWEGVMDDEPGK SQDYGLGCMLHVCVRSELRPLIFAQKVQLTYYIS Q*DQPVSVHCLCLAPMPARLL*QVPIA**KCEQ PFLWTLGNLVWWIKMDGC*TI

Frame 1

HVLNLGTTNL*ID*DLSQILLGLQDGG*N*RER SSLNSQRSRMGRGLSENSLNTGENRSPESGWPS PRSHSQVHPSSAGSRFPAPVLFLQPMPPVFMLSQ FTFQLHTCNQKQKV*ECVWSQRGDSSGEASFMS CDLFSPFLFGL*S*VDSNLEQCQHLVGLCCLGE VTQSILKEQVKNFSQGIN*EKIK*KQTVPHLG*A PNTFFFFFLRLSLALSLRLECSGAISAHCSLHL PGSSDSSASASQVAGITGAHHHARLIFVFLVET RFHHVGQAGLELLTK*STCFGLPKCWDYRHEPPH LASNKLFNCIQKCTN*AQTSGMETEWEVLGVWP GLAEIRELWGPGSHCASDRSFSSRQQKACQCS* NKKGLFLSLNRSFQHWFIRSGH*AGIPIYTVFLS FTFVRCLAQL*ASNICAMWEHTRGQVGNRVSSV LCPQAGFSRGRRTPTAPGLITTLLVNCRCCHGF LPSYMKCSLAAGLLFVYRTHSSAASWLHQ*AIAP *QATPKCSKHLLLPTCVKSVECFCQPGPGLAHL G*ACSGARVS*TGRQETSCSRRAWTG*IGSPST CILHPSSQGFEPRSRLTPNAPRGWSPE*HRGRGP WMDAWGRLEARHKSYLHSRRGDHAKDKLQRDNR FTSQTHSHWAKSSCHCRFESRPFFLPSPSWSYN RSWGGESAERTWMKQRGTCLSHSPLDLLHPGLCQ HKVGAR*IFIEEMKGDKRQKEGDPKKRDGIPLY CTGWSQTPELKRSSCLGLPRC*DYRCEPPLPA* FIFYKEATMEYTAEQSLAFLDLEHQEA*GG*KLR RKLRVETFDFLPRESS*HTL*PKRPYKYFRAEA AFLHWINKGTVISS*FIFITSNSFPEEKFIFQI YFPSQRGGEL*YSVSIAPFLKIKPRQAFLQHTLP YVIANPRNLEETEVQRG*GMCSPSQSQRSARTQ IQAWLSHPQGRTLYAVPYDFSCSRCKAARIWLF *HLSGTWYVPGLL*TPR*HGPCLSIQHQRDCYQD LRPETQP*IRCRPLVFAKDA*VCVCVCVHCV*G CVYVGGWGQKMLPELLVLRQIFDGGTGVWRLEP *FQSGDLEH*YGQQTGWSC*IVS*VFNPLPFSKS GWNWTQGWFRAEVSGG*MIPDSGKG*WMMNLAS HRTMDLAVCCMYV*DLS*DL*FLPRKSS*HTI* ASETSQCLFIASALPLCLPASYSRSL*HNENANN LFSGHWGI*SGG*RWMAVKR

Frame 2

MSLTLAQQISKSIETCLRYFLVYKMEAEIKEKD PHSTHRGAEWEEDLVRIP*TQEKTEAQRVDGQV QGHTAKCIRAAQDPGFLPQCSFCSPCHLFSCYL SSPSSYTPAIRNRKSESVCGAKEGTAVVRPVSCL VIYFLPFFLDFNLKWTAIWNSVSTLLDCAVWER *HRVF*KSRLRTFHKESIERK*NRNRLSHIWAE HQTHFFFFF*D*VLLCHSGWNAVARSRLTAAFTS RVQVILLPQPPK*LGLQVPTTMPG*FLYF**RR GFTMLARLVLNS*PSDPPASASQSAGITGTSHR TWPQTSFLIAFRSAPTRHRPLAWRQNGRFLVSGL VLQKSGSCGDQAATVHQIGVSAAGNRKPANVAE IRRAYFYP*TEASSTGSSDQGTELASPFIQFSF PSPL*DAWHSSEHQISVPCGSTRGDKWGIGSPLS SVPRLASLVVEGPPQHQAS*LPCW*TAGAVTAS CLRI*NVPWQLDCCLFTARTALLPLGYTSELLL HDKPHRNVASTYCCPHVSSQLSVSANRGLAWPTL AELALVLGSARQVGRRPAALEEPGQDELVLLPR ASCIPPAKDLNPGLV*LQMHQEDGAQSDTEEEA PGWMHGEG*KQGTSHTYIQGEGTMQRINCRETTD LHPRHIHTGPRAAAIADSSHVLFSFLLLLGVTT EAGEVRVQKGRG*SREARACLIRLWICCIQDCA STKWEPDKYLLKK*KVTKGRRRETPKREMGSHYI AQAGLKLLSSSDPPALASHGAETTGVSHHSQPD LYFIKRQRWSTQLSNLLHFWTLSIRKLREGENS EESSELRPLIFCPESPANTLYNPKGLTNISEQRQ HFCTG*TKVR*FLHSLFSLQVILFLKKNLYFRF ISPLKGEGSYNILFP*HHSSRSNPGRHFYSTHF HMSLLILATLRKLRCKGVEECVHHPKANEVPEPK SKLGCLIPKDVLFMPFLMTFHAAGARQQEFGSS DIYQAPGMCQAYCKPQDDMVHVYPFSIRETVIR T*GLRHSPESDAGLWCLQRMREYVYVYACIVCRG VCMLGVGGRKCSLNCWY*GRSLMEGLGCGDLNP SFKVGTSNTSMASRQAGVAELSARSLIHSPSLN QGGIGLRAGSGLRFPGDE*FQTVGRGDG**TWQV TGLWTWLYVACMCKI*VETFDFCPESPANILYK PVRPASVCSLPLPCPYACPPPIAGPYSIMKMRT TFSLDTGESSLVDKDGWLLND

Sequence NM_014418

Frame 0

Frame 1

Frame 2

Sequence NM_012468

Frame 0

Frame 1

Frame 2

Sequence NM_020553

Frame 0

Frame 1

Frame 2

Sequence NM_020550

Frame 0

Frame 1

Frame 2

Sequence NM_020552

Frame 0

Frame 1

Frame 2

Sequence NM_020554

Frame 0

Frame 1

Frame 2