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Sequence NM_001025238
AAAGCTGCGGCGGCGGTTCGCGTTTCTCGTGTCCGCTTGACTGACAGCTGCGCGGCGGGAGCGGGCGGCGCGAGCGGGAGGCGGCGGCGCAGAACTGAAGCGCTGCGGCATGGCGCGCGCCTGCCTCCAGGCCGTCAAGTACCTCATGTTCGCCTTCAACCTGCTCTTCTGGCTGGGAGGCTGTGGCGTGCTGGGTGTCG
GCATCTGGCTGGCCGCCACACAGGGGAGCTTCGCCACGCTGTCCTCTTCCTTCCCGTCCCTGTCGGCTGCCAACTTGCTCATCATCACCGGCGCCTTTGT
CATGGCCATCGGCTTCGTGGGCTGCCTGGGTGCCATCAAGGAGAACAAGTGCCTCCTGCTCACTTTCTTCCTGCTGCTGCTGCTGGTGTTCCTGCTGGAG
GCCACCATCGCCATCCTCTTCTTCGCCTACACGGACAAGATTGACAGGTATGCCCAGCAAGACCTGAAGAAAGGCTTGCACCTGTACGGCACGCAGGGCA
ACGTGGGCCTCACCAACGCCTGGAGCATCATCCAGACCGACTTCCGCTGCTGTGGCGTCTCCAACTACACTGACTGGTTCGAGGTGTACAACGCCACGCG
GGTACCTGACTCCTGCTGCTTGGAGTTCAGTGAGAGCTGTGGGCTGCACGCCCCCGGCACCTGGTGGAAGGCGCCGTGCTACGAGACGGTGAAGGTGTGG
CTTCAGGAGAACCTGCTGGCTGTGGGCATCTTTGGGCTGTGCACGGCGCTGGTGCAGATCCTGGGCCTGACCTTCGCCATGACCATGTACTGCCAAGTGG
TCAAGGCAGACACCTACTGCGCGTAGGCCGCCCACCGCCCGCTTCTCTGCCAAAAGGACGCCCACGGGGAGATGGCCGCACCCACAGCTGCCTTTCCCAC
CACCAGCCTCGGTGCTCTGCCCCATGCTGGGAGGAGGGAGGGAGGGACAGGTGCCTGGAGCCCCCGGAACCCTGTTTCTGGAAGGCCCTAGCTCAGGTGG
CTTCAGGGCCTCCGGACCCCCCCTGGGAGGGGTGGCCACGTGCTGGCTGCGGAACCCAGGGCAGGGGTGGGAGGGGCCTCCAGCACTTTTTATATTTACG
TATTCTCCAAAGCAGTGTTCACACGGGAGCCAGCCTGTGGCCCCCAGCCTCCTGGAAAACAGGTTGGCGCTGGAGGAGCCGGGTCTTGGCATCCTGGAGG
TGGCCCCACTGGTCCTGGTGCTCCAGGCGGGGCCGTGGACCCCTCACCTACATTCCATAGTGGGCCCGTGGGGCTCCTGGTGCATCTTAATAAAGTGTGA
GCAGCAACCTTG
- refseq_TSPAN4.F1 (forward primer)
- refseq_TSPAN4.R1 (reverse primer)
- Single exon skipping
Frame 0
KAAAAVRVSRVRLTDSCAAGAGGASGRRRRRTEALRHGARLPPGRQVPHVRLQPALLAGRLWRAGCR
HLAGRHTGELRHAVLFLPVPVGCQLAHHHRRLC
HGHRLRGLPGCHQGEQVPPAHFLPAAAAGVPAG
GHHRHPLLRLHGQD*QVCPARPEERLAPVRHAGQ
RGPHQRLEHHPDRLPLLWRLQLH*LVRGVQRHA
GT*LLLLGVQ*ELWAARPRHLVEGAVLRDGEGV
ASGEPAGCGHLWAVHGAGADPGPDLRHDHVLPSG
QGRHLLRVGRPPPASLPKGRPRGDGRTHSCLSH
HQPRCSAPCWEEGGRDRCLEPPEPCFWKALAQV
ASGPPDPPWEGWPRAGCGTQGRGGRGLQHFLYLR
ILQSSVHTGASLWPPASWKTGWRWRSRVLASWR
WPHWSWCSRRGRGPLTYIP*WARGAPGAS**SV
SSNL
Frame 1
KLRRRFAFLVSA*LTAARRERAARAGGGGAELKRCGMARACLQAVKYLMFAFNLLFWLGGCGVLGV
GIWLAATQGSFATLSSSFPSLSAANLLIITGAFV
MAIGFVGCLGAIKENKCLLLTFFLLLLLVFLLE
ATIAILFFAYTDKIDRYAQQDLKKGLHLYGTQG
NVGLTNAWSIIQTDFRCCGVSNYTDWFEVYNATR
VPDSCCLEFSESCGLHAPGTWWKAPCYETVKVW
LQENLLAVGIFGLCTALVQILGLTFAMTMYCQV
VKADTYCA*AAHRPLLCQKDAHGEMAAPTAAFPT
TSLGALPHAGRREGGTGAWSPRNPVSGRP*LRW
LQGLRTPPGRGGHVLAAEPRAGVGGASSTFYIY
VFSKAVFTREPACGPQPPGKQVGAGGAGSWHPGG
GPTGPGAPGGAVDPSPTFHSGPVGLLVHLNKV*
AATL
Frame 2
SCGGGSRFSCPLD*QLRGGSGRREREAAAQN*SAAAWRAPASRPSSTSCSPSTCSSGWEAVACWVS
ASGWPPHRGASPRCPLPSRPCRLPTCSSSPAPL
SWPSASWAAWVPSRRTSASCSLSSCCCCWCSCWR
PPSPSSSSPTRTRLTGMPSKT*RKACTCTARRA
TWASPTPGASSRPTSAAVASPTTLTGSRCTTPR
GYLTPAAWSSVRAVGCTPPAPGGRRRATRR*RCG
FRRTCWLWASLGCARRWCRSWA*PSP*PCTAKW
SRQTPTARRPPTARFSAKRTPTGRWPHPQLPFP
PPASVLCPMLGGGREGQVPGAPGTLFLEGPSSGG
FRASGPPLGGVATCWLRNPGQGWEGPPALFIFT
YSPKQCSHGSQPVAPSLLENRLALEEPGLGILE
VAPLVLVLQAGPWTPHLHSIVGPWGSWCILIKCE
QQP
Sequence NM_003271
AAAGCTGCGGCGGCGGTTCGCGTTTCTCGTGTCCGCTTGACTGACAGCTGCGCGGCGGGAGCGGGCGGCGCGAGCGGGAGGCGGCGGCGCAGAGCTTGGGGCTTCCTTGGTCGCACCCACCACCTGCCTGCCCACTGGTCAGCCTTCAGGGACCCTGAGCACCGCCTGGTCTCTTTCCTGTGGCCAGCCCAGAACTGAAG
CGCTGCGGCATGGCGCGCGCCTGCCTCCAGGCCGTCAAGTACCTCATGTTCGCCTTCAACCTGCTCTTCTGGCTGGGAGGCTGTGGCGTGCTGGGTGTCG
GCATCTGGCTGGCCGCCACACAGGGGAGCTTCGCCACGCTGTCCTCTTCCTTCCCGTCCCTGTCGGCTGCCAACTTGCTCATCATCACCGGCGCCTTTGT
CATGGCCATCGGCTTCGTGGGCTGCCTGGGTGCCATCAAGGAGAACAAGTGCCTCCTGCTCACTTTCTTCCTGCTGCTGCTGCTGGTGTTCCTGCTGGAG
GCCACCATCGCCATCCTCTTCTTCGCCTACACGGACAAGATTGACAGGTATGCCCAGCAAGACCTGAAGAAAGGCTTGCACCTGTACGGCACGCAGGGCA
ACGTGGGCCTCACCAACGCCTGGAGCATCATCCAGACCGACTTCCGCTGCTGTGGCGTCTCCAACTACACTGACTGGTTCGAGGTGTACAACGCCACGCG
GGTACCTGACTCCTGCTGCTTGGAGTTCAGTGAGAGCTGTGGGCTGCACGCCCCCGGCACCTGGTGGAAGGCGCCGTGCTACGAGACGGTGAAGGTGTGG
CTTCAGGAGAACCTGCTGGCTGTGGGCATCTTTGGGCTGTGCACGGCGCTGGTGCAGATCCTGGGCCTGACCTTCGCCATGACCATGTACTGCCAAGTGG
TCAAGGCAGACACCTACTGCGCGTAGGCCGCCCACCGCCCGCTTCTCTGCCAAAAGGACGCCCACGGGGAGATGGCCGCACCCACAGCTGCCTTTCCCAC
CACCAGCCTCGGTGCTCTGCCCCATGCTGGGAGGAGGGAGGGAGGGACAGGTGCCTGGAGCCCCCGGAACCCTGTTTCTGGAAGGCCCTAGCTCAGGTGG
CTTCAGGGCCTCCGGACCCCCCCTGGGAGGGGTGGCCACGTGCTGGCTGCGGAACCCAGGGCAGGGGTGGGAGGGGCCTCCAGCACTTTTTATATTTACG
TATTCTCCAAAGCAGTGTTCACACGGGAGCCAGCCTGTGGCCCCCAGCCTCCTGGAAAACAGGTTGGCGCTGGAGGAGCCGGGTCTTGGCATCCTGGAGG
TGGCCCCACTGGTCCTGGTGCTCCAGGCGGGGCCGTGGACCCCTCACCTACATTCCATAGTGGGCCCGTGGGGCTCCTGGTGCATCTTAATAAAGTGTGA
GCAGCAACCTTG
- refseq_TSPAN4.F1 (forward primer)
- refseq_TSPAN4.R1 (reverse primer)
- Single exon skipping
Frame 0
KAAAAVRVSRVRLTDSCAAGAGGASGRRRRRAWGFLGRTHHLPAHWSAFRDPEHRLVSFLWPAQN*S
AAAWRAPASRPSSTSCSPSTCSSGWEAVACWVS
ASGWPPHRGASPRCPLPSRPCRLPTCSSSPAPL
SWPSASWAAWVPSRRTSASCSLSSCCCCWCSCWR
PPSPSSSSPTRTRLTGMPSKT*RKACTCTARRA
TWASPTPGASSRPTSAAVASPTTLTGSRCTTPR
GYLTPAAWSSVRAVGCTPPAPGGRRRATRR*RCG
FRRTCWLWASLGCARRWCRSWA*PSP*PCTAKW
SRQTPTARRPPTARFSAKRTPTGRWPHPQLPFP
PPASVLCPMLGGGREGQVPGAPGTLFLEGPSSGG
FRASGPPLGGVATCWLRNPGQGWEGPPALFIFT
YSPKQCSHGSQPVAPSLLENRLALEEPGLGILE
VAPLVLVLQAGPWTPHLHSIVGPWGSWCILIKCE
QQP
Frame 1
KLRRRFAFLVSA*LTAARRERAARAGGGGAELGASLVAPTTCLPTGQPSGTLSTAWSLSCGQPRTE
ALRHGARLPPGRQVPHVRLQPALLAGRLWRAGCR
HLAGRHTGELRHAVLFLPVPVGCQLAHHHRRLC
HGHRLRGLPGCHQGEQVPPAHFLPAAAAGVPAG
GHHRHPLLRLHGQD*QVCPARPEERLAPVRHAGQ
RGPHQRLEHHPDRLPLLWRLQLH*LVRGVQRHA
GT*LLLLGVQ*ELWAARPRHLVEGAVLRDGEGV
ASGEPAGCGHLWAVHGAGADPGPDLRHDHVLPSG
QGRHLLRVGRPPPASLPKGRPRGDGRTHSCLSH
HQPRCSAPCWEEGGRDRCLEPPEPCFWKALAQV
ASGPPDPPWEGWPRAGCGTQGRGGRGLQHFLYLR
ILQSSVHTGASLWPPASWKTGWRWRSRVLASWR
WPHWSWCSRRGRGPLTYIP*WARGAPGAS**SV
SSNL
Frame 2
SCGGGSRFSCPLD*QLRGGSGRREREAAAQSLGLPWSHPPPACPLVSLQGP*APPGLFPVASPELK
RCGMARACLQAVKYLMFAFNLLFWLGGCGVLGV
GIWLAATQGSFATLSSSFPSLSAANLLIITGAFV
MAIGFVGCLGAIKENKCLLLTFFLLLLLVFLLE
ATIAILFFAYTDKIDRYAQQDLKKGLHLYGTQG
NVGLTNAWSIIQTDFRCCGVSNYTDWFEVYNATR
VPDSCCLEFSESCGLHAPGTWWKAPCYETVKVW
LQENLLAVGIFGLCTALVQILGLTFAMTMYCQV
VKADTYCA*AAHRPLLCQKDAHGEMAAPTAAFPT
TSLGALPHAGRREGGTGAWSPRNPVSGRP*LRW
LQGLRTPPGRGGHVLAAEPRAGVGGASSTFYIY
VFSKAVFTREPACGPQPPGKQVGAGGAGSWHPGG
GPTGPGAPGGAVDPSPTFHSGPVGLLVHLNKV*
AATL
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