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Sequences

Sequence NM_080717

CATGCCTTATGCAAGAGACCTCAGTCCCCCGGAACAACTCGATTTCCTTCCAATAGAGGTCTGAGGTGGACTCCCACCTCCCTTCGTGAAGAGTTCCCTC
CTCTCCCCCTTCCTAAGAAAGTCGATCTTGGCTCTATTTGTGTCTTATGTTCATCACCCTCATTCCTCCGGAGAAAGCCGGGTTGGTTTATGTCTTTATT
TATTCCCGGGGCCAAGACGTCCGGAACCTGTGGCTGCGCAGACCCGGCACTGATAGGCGAAGACGGAGAGAAATTTACCTCCCGCCGCTGCCCCCCAGCC
AAACGTGACAGCGCGCGGGCCGGTTGCGTGACTCGTGACGTCTCCAAGTCCTATAGGTGCAGCGGCTGGTGAGATAGTCGCTATCGCCTGGTTGCCTCTT
TATTTTACTGGGGTATGCCTGGTAATAAACAGTAATATTTAATTTGTCGGAGACCACAAACCAACCTTGAGCTGGGAGGTACGTGCTCTTCTTGACAGAC
GTTGG
AAGAAGACCTGGCCTAAAGAGGTCTCTTTTGGTGGTCCTTTTCAAAGTCTTCACCTGAGCCCTGCTCTCCAGCGAGGCGCACTCCTGGCTTTTGC
GCTCCAAAGAAGAGGTGGGATAGTTGGAGGGCATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGA
AATGATCATAACCAAGGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAAAACGAAGTACATTCTTCTCATGGACATT
GTACCTGCCGACGATCACAGATACAAATTCGCAGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAG
ACTCCCCCGCCACCGGGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACCACCTGGACCCATTTGGGCATATTATTCT
AAATTCCATGCACAAATACCAGCCTAGATTACACATCGTGAAAGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTT
CCTGAGACTGCGTTTATAGCAGTGACTTCCTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCAAAGGATTTCGGGGCAGTG
ATGACATGGAGCTGCACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGTGGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCC
TTTCAGCAGCGAGTCTCGAGCTCTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACCTCCTGCCT
CCACCCAACCCATACCCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAAAGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGA
AGCCCTACATGGAGACATCACCCAGTGAAGAAGATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGC
ACAGCGGCAAGCTTGCATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAGCTGCAACACGTGGCCAAGCATGCCTTCC
TACAGCAGCTGCACCGTCACCACCGTGCAGCCCATGGACAGGCTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGG
CTGGCATGGCCAACCATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGTCAGGCAGTGTGGGCCTCA
GACTGGCCTGCAGTCCCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCTCATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCAC
GGAGTTGGCATGGTGCCAGAGTGGAGCGACAATAGCTAAAGTGAGGCCTGCTTCACAACAGACATTTCCTAGAGAAAGAGAGAGAGAGAGGAGAAAGAGA
GAGAAGGAGAGAGACAGTAGCCAAGAGAACCCCACGGACAAGATTTTTCATTTCACCCAATGTTCACATCTGCACTCAAGGTCGCTGGATGCTGATCTAA
TCAGTAGCTTGAAACCACAATTTTAAAAATGTGACTTTCTTGTTTTGTCTCAAAACTTAAAAAAACAAACACAAAAAGATGAGTCCCACCCCCCACTACC
ACCACACCCATCAACCAGCCACATTCACGCTACTCCCCAGATCTCTTCCCCCATTCCTTCTTTTGGGCTCTAGAAAGTCTTGCCTCATTGAGTGTTTTTC
CCTAGTGCGTAGTTGGAGTCTGTCCCTGTCTTGGTGTTAATGTTGACATTGTTATATAATAAATGATAATATATTTTTTTCTTTCAATTTTCTTAATGGG
ACCCAGTCCCTTATTTGGGGGGAGGTCTGAGGCAAGTATATTTCAAAATATGTACTTGCGGGATTCCCTTCAAGTAAACCATCCCTGAAACCTAAATTCA
CGTTTCCCCTTGACTAAGAAAAGCACCTACCTCTGCCATGTGATGTTTCTGAAAAGCCTCTGTATGTCCCCATTTGCTTTGGTTTTGTCCTGCCTTCTCC
AATATCACGTGCTCAGTTTTGCCTCTACTTACCCATGGAGTCAGGATAACACTGACGCTCCCTGGCATCCTATCTTATTCAGCCCTACCATCTTGCCAGC
TCTGTCTTTCCAGCTGTCTGTCGCTAAAACGTGGCCTATAGCTTCCCTTCCGGAAAGCTTGCTTTGAAAAACTTAAAAAGCCCCCGTTTACATGTAGGCA
GGACTGTGATAACAGTGCAAGCTCTGTGTTGACAAGAGTTGTGGACAAAAAGCCAAAATAAATATTCTTCCTGATTAAAAAAATTTTTTTTGAAAAAAAC
AAGGCCAGCCCCAACCTTCCAAACCTCCATCACCAACAACCCAAACTGGATGTCAAGCAAAATGCACAATTCCTACAGAAGAGGCAAGACACAGTCACCA
ATGATATCTCGCCAAAGAAACCACGCCCACACCAATGCCAACACAAAACTGTGTTTACTGAAAGCCGAAAACAGTATTAAAAAAAGTGTGTAAGTAAAGT
GTTATGGTAGGGTTCTTCAGATGTAATATTTTACTGGTACTATTTATTTATAAATAGGAATTCTAATTAAGTAATAACATGAAATGAAACCCAGCATAGG
AGCTGGCCAAGAGCTTTTAATTTTATTGATACTCAAAACCAAGTTTGTGTTTTTTTGTTTTTTTTTGTTTTTTTCCTCTTTCGAATGTGCTTTGCTTTTT
TTGATTAAAAAGAATTTTTTTTTTCCTTTTTTATAAACAGACCCTAATAAAGAGAACAGGGTAAGATGTGAGGCTGAGTGTGTTTAAGTACGTGAGAGAG
TGTGAGTGTGTTTGTAAGTGAGTGTCCCTATGCGATTATGTCTCTTTACGTTGCTAAGGGGGGAGGGTGAGGATTAAGTACTCGTGCCTTATATTTGTGT
GCCAATTAATGCCTAATAAATACCATGTGCTTAAACAAGTA
  • refseq_TBX5.F1 (forward primer)
  • refseq_TBX5.R1 (reverse primer)
  • Single exon skipping

Frame 0

HALCKRPQSPGTTRFPSNRGLRWTPTSLREEFP
PLPLPKKVDLGSICVLCSSPSFLRRKPGWFMSLF
IPGAKTSGTCGCADPALIGEDGEKFTSRRCPPA
KRDSARAGCVTRDVSKSYRCSGW*DSRYRLVAS
LFYWGMPGNKQ*YLICRRPQTNLELGGTCSS*QT
L
EEDLA*RGLFWWSFSKSSPEPCSPARRTPGFC
APKKRWDSWRAWRESKCFSMKENCG*NSTKWAR
K*S*PRLEGGCFPVTK*R*RALIPKRSTFFSWTL
YLPTITDTNSQIINGL*RAKLSPPCLAACTCTQ
TPPPPGRIG*GSSSPSRNSSSPTTTWTHLGILF
*IPCTNTSLDYTS*KRMKIMDLAQKIQRSALTSF
LRLRL*Q*LPTRTTRSRN*RLRIIPLPKDFGAV
MTWSCTECQECKVKNIPWSPGAP*GKKWPPTTV
LSAASLELSPPHPIWGPNTSVRMVFPAPPRTSCL
HPTHTHCPRSIAKFTIVPRGKRKNVPPQTIPIR
SPTWRHHPVKKIPSTALAIHSSRAWVPPTGQSR
HSGKLACMPALRPPASLCPA*RTSAATRGQACLP
TAAAPSPPCSPWTGYPTSTSPLTSPRGPWSLGW
LAWPTMAPHSWERECSSTRPPWPTSLWSGSVGL
RLACSPLAPFSPLSSSTLMACQGLYPLISTTLCT
ELAWCQSGATIAKVRPASQQTFPRERERERRKR
EKERDSSQENPTDKIFHFTQCSHLHSRSLDADL
ISSLKPQF*KCDFLVLSQNLKKQTQKDESHPPLP
PHPSTSHIHATPQISSPIPSFGL*KVLPH*VFF
PSA*LESVPVLVLMLTLLYNK**YIFFFQFS*W
DPVPYLGGGLRQVYFKICTCGIPFK*TIPET*IH
VSP*LRKAPTSAM*CF*KASVCPHLLWFCPAFS
NITCSVLPLLTHGVRITLTLPGILSYSALPSCQ
LCLSSCLSLKRGL*LPFRKACFEKLKKPPFTCRQ
DCDNSASSVLTRVVDKKPK*IFFLIKKIFFEKN
KASPNLPNLHHQQPKLDVKQNAQFLQKRQDTVT
NDISPKKPRPHQCQHKTVFTESRKQY*KKCVSKV
LW*GSSDVIFYWYYLFINRNSN*VIT*NETQHR
SWPRAFNFIDTQNQVCVFLFFFVFFLFRMCFAF
FD*KEFFFSFFINRP**REQGKM*G*VCLST*ES
VSVFVSECPYAIMSLYVAKGGG*GLSTRALYLC
AN*CLINTMCLNK

Frame 1

MPYARDLSPPEQLDFLPIEV*GGLPPPFVKSSL
LSPFLRKSILALFVSYVHHPHSSGESRVGLCLY
LFPGPRRPEPVAAQTRH**AKTERNLPPAAAPQP
NVTARGPVA*LVTSPSPIGAAAGEIVAIAWLPL
YFTGVCLVINSNI*FVGDHKPTLSWEVRALLDR
RW
KKTWPKEVSFGGPFQSLHLSPALQRGALLAFA
LQRRGGIVGGHGGNQSVSP*KRTVAKIPRSGHG
NDHNQGWKADVSQLQSEGDGP*SQNEVHSSHGH
CTCRRSQIQIRR**MVCDGQS*ARHAWPPVRAPR
LPRHRGALDEAARLLPETQAHQQPPGPIWAYYS
KFHAQIPA*ITHRESG*K*WIWLKKYSVLHSRL
S*DCVYSSDFLPEPQDHAIKD*E*SLCQRISGQ*
*HGAAQNVKNAK*RISRGPQEHREAKSGLQPQS
FQQRVSSSLHLIQFGVPIPV*EWCFRPLPGPPA
STQPIPTAPGA*PNLPLYQEERGRMFHHRPSL*E
ALHGDITQ*RRFLLPL*LSTAAGPGCLLQDRVG
TAASLHVCQLCAPQRACAQPRGHQLQHVAKHAF
LQQLHRHHRAAHGQATLPALLRSLHLGAPGPSAG
WHGQPWLPTAGRGNVPAPDLRGPPACGQAVWAS
DWPAVPWHPSAP*VPLLSWRAKDSIPSSVPLCA
RSWHGARVERQ*LK*GLLHNRHFLEKERERGERE
RRRETVAKRTPRTRFFISPNVHICTQGRWMLI*
SVA*NHNFKNVTFLFCLKT*KNKHKKMSPTPHY
HHTHQPATFTLLPRSLPPFLLLGSRKSCLIECFS
LVRSWSLSLSWC*C*HCYIINDNIFFSFNFLNG
TQSLIWGEV*GKYISKYVLAGFPSSKPSLKPKF
TFPLD*EKHLPLPCDVSEKPLYVPICFGFVLPSP
ISRAQFCLYLPMESG*H*RSLASYLIQPYHLAS
SVFPAVCR*NVAYSFPSGKLALKNLKSPRLHVG
RTVITVQALC*QELWTKSQNKYSS*LKKFFLKKT
RPAPTFQTSITNNPNWMSSKMHNSYRRGKTQSP
MISRQRNHAHTNANTKLCLLKAENSIKKSV*VK
CYGRVLQM*YFTGTIYL*IGILIK**HEMKPSIG
AGQELLILLILKTKFVFFCFFLFFSSFECALLF
LIKKNFFFPFL*TDPNKENRVRCEAECV*VRER
V*VCL*VSVPMRLCLFTLLRGEGED*VLVPYICV
PINA**IPCA*TS

Frame 2

CLMQETSVPRNNSISFQ*RSEVDSHLPS*RVPS
SPPS*ESRSWLYLCLMFITLIPPEKAGLVYVFI
YSRGQDVRNLWLRRPGTDRRRRREIYLPPLPPS
QT*QRAGRLRDS*RLQVL*VQRLVR*SLSPGCLF
ILLGYAW**TVIFNLSETTNQP*AGRYVLFLTD
VG
RRPGLKRSLLVVLFKVFT*ALLSSEAHSWLL
RSKEEVG*LEGMEGIKVFLHERELWLKFHEVGTE
MIITKAGRRMFPSYKVKVTGLNPKTKYILLMDI
VPADDHRYKFADNKWSVTGKAEPAMPGRLYVHP
DSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIIL
NSMHKYQPRLHIVKADENNGFGSKNTAFCTHVF
PETAFIAVTSYQNHKITQLKIENNPFAKGFRGS
DDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSP
FSSESRALSTSSNLGSQYQCENGVSGPSQDLLP
PPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYK
KPYMETSPSEEDSFYRSSYPQQQGLGASYRTESA
QRQACMYASSAPPSEPVPSLEDISCNTWPSMPS
YSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRL
AGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQ
TGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVH
GVGMVPEWSDNS*SEACFTTDIS*RKREREEKE
REGERQ*PREPHGQDFSFHPMFTSALKVAGC*SN
Q*LETTILKM*LSCFVSKLKKTNTKR*VPPPTT
TTPINQPHSRYSPDLFPHSFFWALESLASLSVF
P*CVVGVCPCLGVNVDIVI**MIIYFFLSIFLMG
PSPLFGGRSEASIFQNMYLRDSLQVNHP*NLNS
RFPLTKKSTYLCHVMFLKSLCMSPFALVLSCLL
QYHVLSFASTYPWSQDNTDAPWHPILFSPTILPA
LSFQLSVAKTWPIASLPESLL*KT*KAPVYM*A
GL**QCKLCVDKSCGQKAKINILPD*KNFF*KK
QGQPQPSKPPSPTTQTGCQAKCTIPTEEARHSHQ
*YLAKETTPTPMPTQNCVY*KPKTVLKKVCK*S
VMVGFFRCNILLVLFIYK*EF*LSNNMK*NPA*
ELAKSF*FY*YSKPSLCFFVFFCFFPLSNVLCFF
*LKRIFFFLFYKQTLIKRTG*DVRLSVFKYVRE
CECVCK*VSLCDYVSLRC*GGRVRIKYSCLIFV
CQLMPNKYHVLKQV

Sequence NM_000192

CATGCCTTATGCAAGAGACCTCAGTCCCCCGGAACAACTCGATTTCCTTCCAATAGAGGTCTGAGGTGGACTCCCACCTCCCTTCGTGAAGAGTTCCCTC
CTCTCCCCCTTCCTAAGAAAGTCGATCTTGGCTCTATTTGTGTCTTATGTTCATCACCCTCATTCCTCCGGAGAAAGCCGGGTTGGTTTATGTCTTTATT
TATTCCCGGGGCCAAGACGTCCGGAACCTGTGGCTGCGCAGACCCGGCACTGATAGGCGAAGACGGAGAGAAATTTACCTCCCGCCGCTGCCCCCCAGCC
AAACGTGACAGCGCGCGGGCCGGTTGCGTGACTCGTGACGTCTCCAAGTCCTATAGGTGCAGCGGCTGGTGAGATAGTCGCTATCGCCTGGTTGCCTCTT
TATTTTACTGGGGTATGCCTGGTAATAAACAGTAATATTTAATTTGTCGGAGACCACAAACCAACCTTGAGCTGGGAGGTACGTGCTCTTCTTGACAGAC
GTTGG
AAGAAGACCTGGCCTAAAGAGGTCTCTTTTGGTGGTCCTTTTCAAAGTCTTCACCTGAGCCCTGCTCTCCAGCGAGGCGCACTCCTGGCTTTTGC
GCTCCAAAGAAGAGGTGGGATAGTTGGAGAGCAGAACCTTGCGCGGGCACAGGGCCCTGGGCGCACCATGGCCGACGCAGACGAGGGCTTTGGCCTGGCG
CACACGCCTCTGGAGCCTGACGCAAAAGACCTGCCCTGCGATTCGAAACCCGAGAGCGCGCTCGGGGCCCCCAGCAAGTCCCCGTCGTCCCCGCAGGCCG
CCTTCACCCAGCAG
GGCATGGAGGGAATCAAAGTGTTTCTCCATGAAAGAGAACTGTGGCTAAAATTCCACGAAGTGGGCACGGAAATGATCATAACCAA
GGCTGGAAGGCGGATGTTTCCCAGTTACAAAGTGAAGGTGACGGGCCTTAATCCCAAAACGAAGTACATTCTTCTCATGGACATTGTACCTGCCGACGAT
CACAGATACAAATTCGCAGATAATAAATGGTCTGTGACGGGCAAAGCTGAGCCCGCCATGCCTGGCCGCCTGTACGTGCACCCAGACTCCCCCGCCACCG
GGGCGCATTGGATGAGGCAGCTCGTCTCCTTCCAGAAACTCAAGCTCACCAACAACCACCTGGACCCATTTGGGCATATTATTCTAAATTCCATGCACAA
ATACCAGCCTAGATTACACATCGTGAAAGCGGATGAAAATAATGGATTTGGCTCAAAAAATACAGCGTTCTGCACTCACGTCTTTCCTGAGACTGCGTTT
ATAGCAGTGACTTCCTACCAGAACCACAAGATCACGCAATTAAAGATTGAGAATAATCCCTTTGCCAAAGGATTTCGGGGCAGTGATGACATGGAGCTGC
ACAGAATGTCAAGAATGCAAAGTAAAGAATATCCCGTGGTCCCCAGGAGCACCGTGAGGCAAAAAGTGGCCTCCAACCACAGTCCTTTCAGCAGCGAGTC
TCGAGCTCTCTCCACCTCATCCAATTTGGGGTCCCAATACCAGTGTGAGAATGGTGTTTCCGGCCCCTCCCAGGACCTCCTGCCTCCACCCAACCCATAC
CCACTGCCCCAGGAGCATAGCCAAATTTACCATTGTACCAAGAGGAAAGAGGAAGAATGTTCCACCACAGACCATCCCTATAAGAAGCCCTACATGGAGA
CATCACCCAGTGAAGAAGATTCCTTCTACCGCTCTAGCTATCCACAGCAGCAGGGCCTGGGTGCCTCCTACAGGACAGAGTCGGCACAGCGGCAAGCTTG
CATGTATGCCAGCTCTGCGCCCCCCAGCGAGCCTGTGCCCAGCCTAGAGGACATCAGCTGCAACACGTGGCCAAGCATGCCTTCCTACAGCAGCTGCACC
GTCACCACCGTGCAGCCCATGGACAGGCTACCCTACCAGCACTTCTCCGCTCACTTCACCTCGGGGCCCCTGGTCCCTCGGCTGGCTGGCATGGCCAACC
ATGGCTCCCCACAGCTGGGAGAGGGAATGTTCCAGCACCAGACCTCCGTGGCCCACCAGCCTGTGGTCAGGCAGTGTGGGCCTCAGACTGGCCTGCAGTC
CCCTGGCACCCTTCAGCCCCCTGAGTTCCTCTACTCTCATGGCGTGCCAAGGACTCTATCCCCTCATCAGTACCACTCTGTGCACGGAGTTGGCATGGTG
CCAGAGTGGAGCGACAATAGCTAAAGTGAGGCCTGCTTCACAACAGACATTTCCTAGAGAAAGAGAGAGAGAGAGGAGAAAGAGAGAGAAGGAGAGAGAC
AGTAGCCAAGAGAACCCCACGGACAAGATTTTTCATTTCACCCAATGTTCACATCTGCACTCAAGGTCGCTGGATGCTGATCTAATCAGTAGCTTGAAAC
CACAATTTTAAAAATGTGACTTTCTTGTTTTGTCTCAAAACTTAAAAAAACAAACACAAAAAGATGAGTCCCACCCCCCACTACCACCACACCCATCAAC
CAGCCACATTCACGCTACTCCCCAGATCTCTTCCCCCATTCCTTCTTTTGGGCTCTAGAAAGTCTTGCCTCATTGAGTGTTTTTCCCTAGTGCGTAGTTG
GAGTCTGTCCCTGTCTTGGTGTTAATGTTGACATTGTTATATAATAAATGATAATATATTTTTTTCTTTCAATTTTCTTAATGGGACCCAGTCCCTTATT
TGGGGGGAGGTCTGAGGCAAGTATATTTCAAAATATGTACTTGCGGGATTCCCTTCAAGTAAACCATCCCTGAAACCTAAATTCACGTTTCCCCTTGACT
AAGAAAAGCACCTACCTCTGCCATGTGATGTTTCTGAAAAGCCTCTGTATGTCCCCATTTGCTTTGGTTTTGTCCTGCCTTCTCCAATATCACGTGCTCA
GTTTTGCCTCTACTTACCCATGGAGTCAGGATAACACTGACGCTCCCTGGCATCCTATCTTATTCAGCCCTACCATCTTGCCAGCTCTGTCTTTCCAGCT
GTCTGTCGCTAAAACGTGGCCTATAGCTTCCCTTCCGGAAAGCTTGCTTTGAAAAACTTAAAAAGCCCCCGTTTACATGTAGGCAGGACTGTGATAACAG
TGCAAGCTCTGTGTTGACAAGAGTTGTGGACAAAAAGCCAAAATAAATATTCTTCCTGATTAAAAAAATTTTTTTTGAAAAAAACAAGGCCAGCCCCAAC
CTTCCAAACCTCCATCACCAACAACCCAAACTGGATGTCAAGCAAAATGCACAATTCCTACAGAAGAGGCAAGACACAGTCACCAATGATATCTCGCCAA
AGAAACCACGCCCACACCAATGCCAACACAAAACTGTGTTTACTGAAAGCCGAAAACAGTATTAAAAAAAGTGTGTAAGTAAAGTGTTATGGTAGGGTTC
TTCAGATGTAATATTTTACTGGTACTATTTATTTATAAATAGGAATTCTAATTAAGTAATAACATGAAATGAAACCCAGCATAGGAGCTGGCCAAGAGCT
TTTAATTTTATTGATACTCAAAACCAAGTTTGTGTTTTTTTGTTTTTTTTTGTTTTTTTCCTCTTTCGAATGTGCTTTGCTTTTTTTGATTAAAAAGAAT
TTTTTTTTTCCTTTTTTATAAACAGACCCTAATAAAGAGAACAGGGTAAGATGTGAGGCTGAGTGTGTTTAAGTACGTGAGAGAGTGTGAGTGTGTTTGT
AAGTGAGTGTCCCTATGCGATTATGTCTCTTTACGTTGCTAAGGGGGGAGGGTGAGGATTAAGTACTCGTGCCTTATATTTGTGTGCCAATTAATGCCTA
ATAAATACCATGTGCTTAAACAAGTA
  • refseq_TBX5.F1 (forward primer)
  • refseq_TBX5.R1 (reverse primer)
  • Single exon skipping

Frame 0

HALCKRPQSPGTTRFPSNRGLRWTPTSLREEFP
PLPLPKKVDLGSICVLCSSPSFLRRKPGWFMSLF
IPGAKTSGTCGCADPALIGEDGEKFTSRRCPPA
KRDSARAGCVTRDVSKSYRCSGW*DSRYRLVAS
LFYWGMPGNKQ*YLICRRPQTNLELGGTCSS*QT
L
EEDLA*RGLFWWSFSKSSPEPCSPARRTPGFC
APKKRWDSWRAEPCAGTGPWAHHGRRRRGLWPG
AHASGA*RKRPALRFETRERARGPQQVPVVPAGR
LHPA
GHGGNQSVSP*KRTVAKIPRSGHGNDHNQ
GWKADVSQLQSEGDGP*SQNEVHSSHGHCTCRR
SQIQIRR**MVCDGQS*ARHAWPPVRAPRLPRHR
GALDEAARLLPETQAHQQPPGPIWAYYSKFHAQ
IPA*ITHRESG*K*WIWLKKYSVLHSRLS*DCV
YSSDFLPEPQDHAIKD*E*SLCQRISGQ**HGAA
QNVKNAK*RISRGPQEHREAKSGLQPQSFQQRV
SSSLHLIQFGVPIPV*EWCFRPLPGPPASTQPI
PTAPGA*PNLPLYQEERGRMFHHRPSL*EALHGD
ITQ*RRFLLPL*LSTAAGPGCLLQDRVGTAASL
HVCQLCAPQRACAQPRGHQLQHVAKHAFLQQLH
RHHRAAHGQATLPALLRSLHLGAPGPSAGWHGQP
WLPTAGRGNVPAPDLRGPPACGQAVWASDWPAV
PWHPSAP*VPLLSWRAKDSIPSSVPLCARSWHG
ARVERQ*LK*GLLHNRHFLEKERERGERERRRET
VAKRTPRTRFFISPNVHICTQGRWMLI*SVA*N
HNFKNVTFLFCLKT*KNKHKKMSPTPHYHHTHQ
PATFTLLPRSLPPFLLLGSRKSCLIECFSLVRSW
SLSLSWC*C*HCYIINDNIFFSFNFLNGTQSLI
WGEV*GKYISKYVLAGFPSSKPSLKPKFTFPLD
*EKHLPLPCDVSEKPLYVPICFGFVLPSPISRAQ
FCLYLPMESG*H*RSLASYLIQPYHLASSVFPA
VCR*NVAYSFPSGKLALKNLKSPRLHVGRTVIT
VQALC*QELWTKSQNKYSS*LKKFFLKKTRPAPT
FQTSITNNPNWMSSKMHNSYRRGKTQSPMISRQ
RNHAHTNANTKLCLLKAENSIKKSV*VKCYGRV
LQM*YFTGTIYL*IGILIK**HEMKPSIGAGQEL
LILLILKTKFVFFCFFLFFSSFECALLFLIKKN
FFFPFL*TDPNKENRVRCEAECV*VRERV*VCL
*VSVPMRLCLFTLLRGEGED*VLVPYICVPINA*
*IPCA*TS

Frame 1

MPYARDLSPPEQLDFLPIEV*GGLPPPFVKSSL
LSPFLRKSILALFVSYVHHPHSSGESRVGLCLY
LFPGPRRPEPVAAQTRH**AKTERNLPPAAAPQP
NVTARGPVA*LVTSPSPIGAAAGEIVAIAWLPL
YFTGVCLVINSNI*FVGDHKPTLSWEVRALLDR
RW
KKTWPKEVSFGGPFQSLHLSPALQRGALLAFA
LQRRGGIVGEQNLARAQGPGRTMADADEGFGLA
HTPLEPDAKDLPCDSKPESALGAPSKSPSSPQA
AFTQQ
GMEGIKVFLHERELWLKFHEVGTEMIITK
AGRRMFPSYKVKVTGLNPKTKYILLMDIVPADD
HRYKFADNKWSVTGKAEPAMPGRLYVHPDSPAT
GAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHK
YQPRLHIVKADENNGFGSKNTAFCTHVFPETAF
IAVTSYQNHKITQLKIENNPFAKGFRGSDDMEL
HRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSES
RALSTSSNLGSQYQCENGVSGPSQDLLPPPNPY
PLPQEHSQIYHCTKRKEEECSTTDHPYKKPYME
TSPSEEDSFYRSSYPQQQGLGASYRTESAQRQAC
MYASSAPPSEPVPSLEDISCNTWPSMPSYSSCT
VTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMAN
HGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQS
PGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMV
PEWSDNS*SEACFTTDIS*RKREREEKEREGER
Q*PREPHGQDFSFHPMFTSALKVAGC*SNQ*LET
TILKM*LSCFVSKLKKTNTKR*VPPPTTTTPIN
QPHSRYSPDLFPHSFFWALESLASLSVFP*CVV
GVCPCLGVNVDIVI**MIIYFFLSIFLMGPSPLF
GGRSEASIFQNMYLRDSLQVNHP*NLNSRFPLT
KKSTYLCHVMFLKSLCMSPFALVLSCLLQYHVL
SFASTYPWSQDNTDAPWHPILFSPTILPALSFQL
SVAKTWPIASLPESLL*KT*KAPVYM*AGL**Q
CKLCVDKSCGQKAKINILPD*KNFF*KKQGQPQ
PSKPPSPTTQTGCQAKCTIPTEEARHSHQ*YLAK
ETTPTPMPTQNCVY*KPKTVLKKVCK*SVMVGF
FRCNILLVLFIYK*EF*LSNNMK*NPA*ELAKS
F*FY*YSKPSLCFFVFFCFFPLSNVLCFF*LKRI
FFFLFYKQTLIKRTG*DVRLSVFKYVRECECVC
K*VSLCDYVSLRC*GGRVRIKYSCLIFVCQLMP
NKYHVLKQV

Frame 2

CLMQETSVPRNNSISFQ*RSEVDSHLPS*RVPS
SPPS*ESRSWLYLCLMFITLIPPEKAGLVYVFI
YSRGQDVRNLWLRRPGTDRRRRREIYLPPLPPS
QT*QRAGRLRDS*RLQVL*VQRLVR*SLSPGCLF
ILLGYAW**TVIFNLSETTNQP*AGRYVLFLTD
VG
RRPGLKRSLLVVLFKVFT*ALLSSEAHSWLL
RSKEEVG*LESRTLRGHRALGAPWPTQTRALAWR
TRLWSLTQKTCPAIRNPRARSGPPASPRRPRRP
PSPSR
AWRESKCFSMKENCG*NSTKWARK*S*P
RLEGGCFPVTK*R*RALIPKRSTFFSWTLYLPTI
TDTNSQIINGL*RAKLSPPCLAACTCTQTPPPP
GRIG*GSSSPSRNSSSPTTTWTHLGILF*IPCT
NTSLDYTS*KRMKIMDLAQKIQRSALTSFLRLRL
*Q*LPTRTTRSRN*RLRIIPLPKDFGAVMTWSC
TECQECKVKNIPWSPGAP*GKKWPPTTVLSAAS
LELSPPHPIWGPNTSVRMVFPAPPRTSCLHPTHT
HCPRSIAKFTIVPRGKRKNVPPQTIPIRSPTWR
HHPVKKIPSTALAIHSSRAWVPPTGQSRHSGKL
ACMPALRPPASLCPA*RTSAATRGQACLPTAAAP
SPPCSPWTGYPTSTSPLTSPRGPWSLGWLAWPT
MAPHSWERECSSTRPPWPTSLWSGSVGLRLACS
PLAPFSPLSSSTLMACQGLYPLISTTLCTELAWC
QSGATIAKVRPASQQTFPRERERERRKREKERD
SSQENPTDKIFHFTQCSHLHSRSLDADLISSLK
PQF*KCDFLVLSQNLKKQTQKDESHPPLPPHPST
SHIHATPQISSPIPSFGL*KVLPH*VFFPSA*L
ESVPVLVLMLTLLYNK**YIFFFQFS*WDPVPY
LGGGLRQVYFKICTCGIPFK*TIPET*IHVSP*L
RKAPTSAM*CF*KASVCPHLLWFCPAFSNITCS
VLPLLTHGVRITLTLPGILSYSALPSCQLCLSS
CLSLKRGL*LPFRKACFEKLKKPPFTCRQDCDNS
ASSVLTRVVDKKPK*IFFLIKKIFFEKNKASPN
LPNLHHQQPKLDVKQNAQFLQKRQDTVTNDISP
KKPRPHQCQHKTVFTESRKQY*KKCVSKVLW*GS
SDVIFYWYYLFINRNSN*VIT*NETQHRSWPRA
FNFIDTQNQVCVFLFFFVFFLFRMCFAFFD*KE
FFFSFFINRP**REQGKM*G*VCLST*ESVSVFV
SECPYAIMSLYVAKGGG*GLSTRALYLCAN*CL
INTMCLNK

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