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Sequences

Sequence NM_014010

GGTTCGCTTCGGCGTCCGCCGTCTCCGCAGCTGCCTCCTCTTCCTCCTTCGCCACCGCCGCCACCGCCGCCGCCGCCCGCAGCACCGCAGCCCCTCCCGC CATGGCCGCCGCCGGCGCCCGGCTCAGCCCCGGCCCCGGCTCGGGGCTCCGGGGGCGGCCGAGGCTCTGCTTCCACCCGGGGCCGCCGCCACTGCTGCCG CTGCTGCTGCTGTTCCTGCTCCTGCTGCCGCCGCCGCCGCTGCTGGCCGGCGCCACCGCCGCTGCCTCGCGGGAGCCCGACAGCCCGTGCCGGCTGAAGA CCGTCACGGTGTCCACACTGCCCGCCCTGCGGGAGAGCGACATCGGCTGGAGCGGCGCCCGCGCCGGGGCCGGGGCTGGGACCGGGGCCGGAGCCGCCGC CGCCGCCGCGTCCCCGGGCTCTCCTGGCTCTGCCGGCACCGCCGCCGAGTCGCGCCTCCTGCTCTTTGTGCGTAACGAGCTGCCGGGGCGCATCGCGGTG CAGGACGACCTGGACAACACCGAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCACAGCAGCGGACATCTCGCTGGTGCACTGGAGACAGCAGTGGCTGG AGAATGGCACCTTGTACTTCCACGTCTCCATGAGCAGCTCCGGGCAGCTGGCCCAAGCCACCGCCCCCACTCTCCAGGAGCCCTCGGAGATTGTTGAGGA GCAGATGCACATCCTCCACATTTCTGTGATGGGTGGCCTCATCGCGCTGCTGCTGCTGCTGCTGGTGTTCACCGTGGCGCTGTACGCCCAGCGACGTTGG CAGAAGCGTCGCCGCATCCCCCAGAAGAGCGCAAGCACAGAAGCCACTCATGAGATCCACTACATCCCATCTGTGCTGCTGGGTCCCCAGGCGCGGGAGA GCTTCCGTTCATCCCGGCTGCAAACCCACAATTCCGTCATTGGCGTGCCCATCCGGGAGACTCCCATCCTGGATGACTATGACTGTGAGGAGGATGAGGA GCCACCTAGGCGGGCCAACCATGTCTCCCGCGAGGACGAGTTTGGCAGCCAGGTGACCCACACTCTGGACAGTCTGGGACATCCAGGGGAAGAGAAGGTG GACTTTGAGAAGAAAGGAGGAATCAGCTTTGGGAGAGCCAAGGGGACGTCGGGCTCAGAGGCAGACGATGAAACTCAGCTGACATTCTACACGGAGCAGT ACCGCAGTCGCCGCCGCAGCAAAGGTTTGCTGAAAAGCCCAGTGAACAAGACAGCCCTGACACTGATTGCTGTGAGTTCCTGCATCCTGGCCATGGTGTG TGGCAGCCAGATGTCTTGTCCACTCACTGTGAAGGTGACTCTGCATGTGCCCGAGCACTTCATAGCAGATGGAAGCAGCTTCGTGGTGAGTGAAGGGAGC TACCTGGACATCTCCGACTGGTTAAACCCGGCCAAGCTTTCCCTGTATTACCAGATCAATGCCACCTCCCCATGGGTGAGGGACCTCTGTGGACAAAGGA CGACAGATGCCTGTGAGCAGCTCTGCGACCCAGAAACCGGAGAGTGCAGCTGTCATGAAGGCTATGCCCCTGACCCTGTTCACAGACACCTGTGTGTGCG CAGTGACTGGGGACAGAGTGAAGGACCTTGGCCCTACACGACACTTGAGAGGGGCTATGATCTGGTGACAGGGGAGCAAGCCCCTGAAAAGATTCTCAGG TCTACTTTCAGCTTGGGCCAAGGCCTCTGGCTTCCTGTCAGCAAAAGCTTTGTGGTTCCGCCTGTGGAGCTGTCCATCAACCCCCTGGCCAGCTGCAAGA CCGATGTGCTCGTCACGGAAGACCCTGCAGATGTCAGGGAAGAAGCGATGCTGTCCACATACTTTGAAACCATCAATGACCTGCTGTCTTCCTTCGGGCC AGTTCGTGACTGCTCTCGGAACAATGGGGGCTGCACTCGCAACTTCAAGTGTGTGTCTGACCGGCAGGTGGATTCCTCGGGATGTGTGTGCCCTGAGGAG CTGAAACCCATGAAGGATGGCTCTGGCTGCTACGACCACTCCAAAGGCATTGACTGCTCTGATGGCTTTAATGGCGGCTGTGAGCAGCTGTGCCTGCAGC AGACGCTGCCCCTGCCCTACGATGCCACTTCGAGCACCATCTTCATGTTCTGCGGTTGCGTGGAGGAGTACAAACTGGCTCCTGATGGAAAATCCTGCTT AATGCTCTCAGATGTCTGCGAGGGCCCCAAGTGCCTCAAACCTGACTCCAAATTCAATGATACCCTCTTTGGAGAGATGCTACATGGTTACAACAACCGG ACCCAGCATGTGAACCAAGGCCAAGTCTTCCAGATGACCTTTAGGGAGAACAACTTCATCAAGGACTTTCCCCAGCTGGCCGATGGGCTGTTGGTGATCC CGCTGCCGGTGGAGGAGCAGTGCCGGGGGGTCCTCTCCGAGCCCCTTCCAGACCTCCAACTGCTCACTGGAGATATCAGGTATGATGAGGCCATGGGTTA CCCCATGGTGCAGCAGTGGCGGGTCCGGAGCAACCTCTACCGTGTGAAGCTCAGCACCATCACCCTCGCAGCAGGCTTCACTAATGTTCTCAAGATCCTG ACCAAGGAGAGCAGTCGGGAGGAGCTGCTGTCCTTCATCCAGCACTATGGCTCCCACTACATCGCAGAGGCCCTCTATGGCTCAGAGCTCACCTGCATCA TCCACTTTCCCAGCAAGAAGGTCCAGCAGCAGCTGTGGCTCCAGTATCAGAAAGAGACCACAGAGCTGGGCAGCAAGAAGGAGCTCAAGTCCATGCCCTT CATCACCTACCTCTCAGGTTTGCTGACAGCCCAGATGCTGTCAGATGACCAGCTCATTTCAGGTGTGGAGATTCGCTGTGAGGAGAAGGGGCGCTGTCCA TCTACCTGTCACCTTTGCCGCCGGCCAGGCAAGGAGCAGCTGAGCCCCACACCAGTGCTGCTGGAAATCAACCGTGTGGTGCCACTTTATACCCTCATCC AAGACAATGGCACAAAGGAGGCCTTCAAGAGTGCACTGATGAGTTCCTACTGGTGCTCAGGGAAAGGGGATGTGATCGATGACTGGTGCAGGTGTGACCT CAGCGCCTTTGATGCCAATGGGCTCCCCAACTGCAGCCCCCTTCTGCAGCCGGTGCTGCGGCTGTCCCCAACAGTGGAGCCCTCCAGTACTGTGGTCTCC TTGGAGTGGGTGGATGTTCAGCCAGCTATTGGGACCAAGGTCTCCGACTATATTCTGCAGCATAAGAAAGTGGATGAATACACAGACACTGACCTGTACA CAGGAGAATTCCTGAGTTTTGCTGATGACTTACTCTCTGGCCTGGGCACATCTTGTGTAGCAGCTGGTCGAAGCCATGGAGAGGTCCCTGAAGTCAGTAT CTACTCAGTCATCTTCAAGTGTCTGGAGCCCGACGGTCTCTACAAGTTCACTCTGTATGCTGTGGATACACGAGGGAGGCACTCAGAGCTAAGCACGGTG ACCCTGAGGACGGCCTGTCCACTGGTAGATGACAACAAGGCAGAAGAAATAGCTGACAAGATCTACAATCTGTACAATGGGTACACAAGTGGAAAGGAGC AGCAGATGGCCTACAACACACTGATGGAGGTCTCAGCCTCGATGCTGTTCCGAGTCCAGCACCACTACAACTCTCACTATGAAAAGTTTGGCGACTTCGT CTGGAGAAGTGAGGATGAGCTGGGGCCCAGGAAGGCCCACCTGATTCTACGGCGACTGGAGAGGGTGAGTAGCCACTGCTCCAGCCTCCTGCGGAGTGCC TACATCCAGAGCCGCGTGGAAACAGTGCCCTATCTTTTCTGCCGCAGCGAGGAGGTCCGGCCTGCAGGCATGGTGTGGTATAGCATCCTCAAGGACACCA AAATCACGTGTGAGGAGAAGATGGTGTCAATGGCCCGAAACACGTACGGGGAGTCCAAGGGCCGGTGAGGGAGGGTATTGCCCTCCGTGAGCACAGAGAC TCTCCATGGGAGGGGGAGCAGTATTCTCCTGGATCCTGGGGCCTGGGTGGGCTGGGGGACAGCTGAGGATGGGCCTAGCAGATGAAGCTTGCCAGCAAGG CCAAAGCAAACGGTTTCTCCTGTGGATAGTGGACAGAGACCTTTGTAACCAATGGAATTATTCATTTTTCTCTATCTTTTATTTTTTCAAAGATATTATT TGACTCTATCAAAAGTCTCTCCTTTTTAAACCTTTTCTTATGGATGGCTGTCAATCCCGAGGCAGAAGTTTTCAGGTGGAGACCAAGCGGCCTTTGCTCT TCTTCCTTCTTCCTGCCACACTCTGCTTTCTTCCTGCCATGGACCCCTGGAGGAGACCTATGGAGGGACAGTTTTGACCTGACCCCTAGAGGAGACAGTT TTGACCTCTTCAGCACCAGGAAGGAAGCTCTGAGGATGGTTGCAGTGAGGAAGCATGGGTCTTTAAGGACTTCTCTCTCTTTTTTGCTGGACATTATTGA GTTTGTGGGACCCTGCCTCTTCCTGCTACCTGTGGGTCTGCCCAGAGTCCCTGCAGGCCTGTCCATGCATTAAAAATTCCTATTGTCTCTC

refseq_ASTN2.F1 (forward primer)
refseq_ASTN2.R1 (reverse primer)
Single exon skipping

Frame 0

GSLRRPPSPQLPPLPPSPPPPPPPPPAAPQPLP PWPPPAPGSAPAPARGSGGGRGSASTRGRRHCCR CCCCSCSCCRRRRCWPAPPPLPRGSPTARAG*R PSRCPHCPPCGRATSAGAAPAPGPGLGPGPEPP PPPRPRALLALPAPPPSRASCSLCVTSCRGASRC RTTWTTPSCPSSPWRCLAQQRTSRWCTGDSSGW RMAPCTSTSP*AAPGSWPKPPPPLSRSPRRLLR SRCTSSTFL*WVASSRCCCCCWCSPWRCTPSDVG RSVAASPRRAQAQKPLMRSTTSHLCCWVPRRGR ASVHPGCKPTIPSLACPSGRLPSWMTMTVRRMR SHLGGPTMSPARTSLAAR*PTLWTVWDIQGKRRW TLRRKEESALGEPRGRRAQRQTMKLS*HSTRSS TAVAAAAKVC*KAQ*TRQP*H*LL*VPASWPWC VAARCLVHSL*R*LCMCPSTS*QMEAASW*VKGA TWTSPTG*TRPSFPCITRSMPPPHG*GTSVDKG RQMPVSSSATQKPESAAVMKAMPLTLFTDTCVC AVTGDRVKDLGPTRHLRGAMIW*QGSKPLKRFSG LLSAWAKASGFLSAKALWFRLWSCPSTPWPAAR PMCSSRKTLQMSGKKRCCPHTLKPSMTCCLPSG QFVTALGTMGAALATSSVCLTGRWIPRDVCALRS *NP*RMALAATTTPKALTALMALMAAVSSCACS RRCPCPTMPLRAPSSCSAVAWRSTNWLLMENPA *CSQMSARAPSASNLTPNSMIPSLERCYMVTTTG PSM*TKAKSSR*PLGRTTSSRTFPSWPMGCW*S RCRWRSSAGGSSPSPFQTSNCSLEISGMMRPWV TPWCSSGGSGATSTV*SSAPSPSQQASLMFSRS* PRRAVGRSCCPSSSTMAPTTSQRPSMAQSSPAS STFPARRSSSSCGSSIRKRPQSWAARRSSSPCP SSPTSQVC*QPRCCQMTSSFQVWRFAVRRRGAVH LPVTFAAGQARSS*APHQCCWKSTVWCHFIPSS KTMAQRRPSRVH**VPTGAQGKGM*SMTGAGVT SAPLMPMGSPTAAPFCSRCCGCPQQWSPPVLWSP WSGWMFSQLLGPRSPTIFCSIRKWMNTQTLTCT QENS*VLLMTYSLAWAHLV*QLVEAMERSLKSV STQSSSSVWSPTVSTSSLCMLWIHEGGTQS*AR* P*GRPVHW*MTTRQKK*LTRSTICTMGTQVERS SRWPTTH*WRSQPRCCSESSTTTTLTMKSLATS SGEVRMSWGPGRPT*FYGDWRG*VATAPASCGVP TSRAAWKQCPIFSAAARRSGLQAWCGIASSRTP KSRVRRRWCQWPETRTGSPRAGEGGYCPP*AQR LSMGGGAVFSWILGPGWAGGQLRMGLADEACQQG QSKRFLLWIVDRDLCNQWNYSFFSIFYFFKDII *LYQKSLLFKPFLMDGCQSRGRSFQVETKRPLL FFLLPATLCFLPAMDPWRRPMEGQF*PDP*RRQF *PLQHQEGSSEDGCSEEAWVFKDFSLFFAGHY* VCGTLPLPATCGSAQSPCRPVHALKIPIVS

Frame 1

VRFGVRRLRSCLLFLLRHRRHRRRRPQHRSPSR HGRRRRPAQPRPRLGAPGAAEALLPPGAAATAA AAAAVPAPAAAAAAAGRRHRRCLAGARQPVPAED RHGVHTARPAGERHRLERRPRRGRGWDRGRSRR RRRVPGLSWLCRHRRRVAPPALCA*RAAGAHRG AGRPGQHRAALLHPGDVWHSSGHLAGALETAVAG EWHLVLPRLHEQLRAAGPSHRPHSPGALGDC*G ADAHPPHFCDGWPHRAAAAAAGVHRGAVRPATL AEASPHPPEERKHRSHS*DPLHPICAAGSPGAGE LPFIPAANPQFRHWRAHPGDSHPG*L*L*GG*G AT*AGQPCLPRGRVWQPGDPHSGQSGTSRGREG GL*EERRNQLWESQGDVGLRGRR*NSADILHGAV PQSPPQQRFAEKPSEQDSPDTDCCEFLHPGHGV WQPDVLSTHCEGDSACARALHSRWKQLRGE*RE LPGHLRLVKPGQAFPVLPDQCHLPMGEGPLWTKD DRCL*AALRPRNRRVQLS*RLCP*PCSQTPVCA Q*LGTE*RTLALHDT*EGL*SGDRGASP*KDSQ VYFQLGPRPLASCQQKLCGSACGAVHQPPGQLQD RCARHGRPCRCQGRSDAVHIL*NHQ*PAVFLRA SS*LLSEQWGLHSQLQVCV*PAGGFLGMCVP*G AETHEGWLWLLRPLQRH*LL*WL*WRL*AAVPAA DAAPALRCHFEHHLHVLRLRGGVQTGS*WKILL NALRCLRGPQVPQT*LQIQ*YPLWRDATWLQQP DPACEPRPSLPDDL*GEQLHQGLSPAGRWAVGDP AAGGGAVPGGPLRAPSRPPTAHWRYQV**GHGL PHGAAVAGPEQPLPCEAQHHHPRSRLH*CSQDP DQGEQSGGAAVLHPALWLPLHRRGPLWLRAHLHH PLSQQEGPAAAVAPVSERDHRAGQQEGAQVHAL HHLPLRFADSPDAVR*PAHFRCGDSL*GEGALS IYLSPLPPARQGAAEPHTSAAGNQPCGATLYPHP RQWHKGGLQECTDEFLLVLRERGCDR*LVQV*P QRL*CQWAPQLQPPSAAGAAAVPNSGALQYCGL LGVGGCSASYWDQGLRLYSAA*ESG*IHRH*PVH RRIPEFC**LTLWPGHILCSSWSKPWRGP*SQY LLSHLQVSGARRSLQVHSVCCGYTREALRAKHG DPEDGLSTGR*QQGRRNS*QDLQSVQWVHKWKGA ADGLQHTDGGLSLDAVPSPAPLQLSL*KVWRLR LEK*G*AGAQEGPPDSTATGEGE*PLLQPPAEC LHPEPRGNSALSFLPQRGGPACRHGVV*HPQGHQ NHV*GEDGVNGPKHVRGVQGPVREGIALREHRD SPWEGEQYSPGSWGLGGLGDS*GWA*QMKLASK AKANGFSCG*WTETFVTNGIIHFSLSFIFSKILF DSIKSLSFLNLFLWMAVNPEAEVFRWRPSGLCS SSFFLPHSAFFLPWTPGGDLWRDSFDLTPRGDS FDLFSTRKEALRMVAVRKHGSLRTSLSFLLDIIE FVGPCLFLLPVGLPRVPAGLSMH*KFLLSL

Frame 2

FASASAVSAAASSSSFATAATAAAARSTAAPPA MAAAGARLSPGPGSGLRGRPRLCFHPGPPPLLP LLLLFLLLLPPPPLLAGATAAASREPDSPCRLK TVTVSTLPALRESDIGWSGARAGAGAGTGAGAAA AAASPGSPGSAGTAAESRLLLFVRNELPGRIAV QDDLDNTELPFFTLEMSGTAADISLVHWRQQWL ENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEE QMHILHISVMGGLIALLLLLLVFTVALYAQRRW QKRRRIPQKSASTEATHEIHYIPSVLLGPQARE SFRSSRLQTHNSVIGVPIRETPILDDYDCEEDEE PPRRANHVSREDEFGSQVTHTLDSLGHPGEEKV DFEKKGGISFGRAKGTSGSEADDETQLTFYTEQ YRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVC GSQMSCPLTVKVTLHVPEHFIADGSSFVVSEGS YLDISDWLNPAKLSLYYQINATSPWVRDLCGQR TTDACEQLCDPETGECSCHEGYAPDPVHRHLCVR SDWGQSEGPWPYTTLERGYDLVTGEQAPEKILR STFSLGQGLWLPVSKSFVVPPVELSINPLASCK TDVLVTEDPADVREEAMLSTYFETINDLLSSFGP VRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEE LKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQ QTLPLPYDATSSTIFMFCGCVEEYKLAPDGKSCL MLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNR TQHVNQGQVFQMTFRENNFIKDFPQLADGLLVI PLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGY PMVQQWRVRSNLYRVKLSTITLAAGFTNVLKIL TKESSREELLSFIQHYGSHYIAEALYGSELTCI IHFPSKKVQQQLWLQYQKETTELGSKKELKSMPF ITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCP STCHLCRRPGKEQLSPTPVLLEINRVVPLYTLI QDNGTKEAFKSALMSSYWCSGKGDVIDDWCRCDL SAFDANGLPNCSPLLQPVLRLSPTVEPSSTVVS LEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLY TGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSI YSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTV TLRTACPLVDDNKAEEIADKIYNLYNGYTSGKE QQMAYNTLMEVSASMLFRVQHHYNSHYEKFGDFV WRSEDELGPRKAHLILRRLERVSSHCSSLLRSA YIQSRVETVPYLFCRSEEVRPAGMVWYSILKDT KITCEEKMVSMARNTYGESKGR*GRVLPSVSTET LHGRGSSILLDPGAWVGWGTAEDGPSR*SLPAR PKQTVSPVDSGQRPL*PMELFIFLYLLFFQRYY LTLSKVSPF*TFSYGWLSIPRQKFSGGDQAAFAL LPSSCHTLLSSCHGPLEETYGGTVLT*PLEETV LTSSAPGRKL*GWLQ*GSMGL*GLLSLFCWTLL SLWDPASSCYLWVCPESLQACPCIKNSYCL

Sequence NM_198187

GGTTCGCTTCGGCGTCCGCCGTCTCCGCAGCTGCCTCCTCTTCCTCCTTCGCCACCGCCGCCACCGCCGCCGCCGCCCGCAGCACCGCAGCCCCTCCCGC CATGGCCGCCGCCGGCGCCCGGCTCAGCCCCGGCCCCGGCTCGGGGCTCCGGGGGCGGCCGAGGCTCTGCTTCCACCCGGGGCCGCCGCCACTGCTGCCG CTGCTGCTGCTGTTCCTGCTCCTGCTGCCGCCGCCGCCGCTGCTGGCCGGCGCCACCGCCGCTGCCTCGCGGGAGCCCGACAGCCCGTGCCGGCTGAAGA CCGTCACGGTGTCCACACTGCCCGCCCTGCGGGAGAGCGACATCGGCTGGAGCGGCGCCCGCGCCGGGGCCGGGGCTGGGACCGGGGCCGGAGCCGCCGC CGCCGCCGCGTCCCCGGGCTCTCCTGGCTCTGCCGGCACCGCCGCCGAGTCGCGCCTCCTGCTCTTTGTGCGTAACGAGCTGCCGGGGCGCATCGCGGTG CAGGACGACCTGGACAACACCGAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCACAGCAGCGGACATCTCGCTGGTGCACTGGAGACAGCAGTGGCTGG AGAATGGCACCTTGTACTTCCACGTCTCCATGAGCAGCTCCGGGCAGCTGGCCCAAGCCACCGCCCCCACTCTCCAGGAGCCCTCGGAGATTGTTGAGGA GCAGATGCACATCCTCCACATTTCTGTGATGGGTGGCCTCATCGCGCTGCTGCTGCTGCTGCTGGTGTTCACCGTGGCGCTGTACGCCCAGCGACGTTGG CAGAAGCGTCGCCGCATCCCCCAGAAGAGCGCAAGCACAGAAGCCACTCATGAGATCCACTACATCCCATCTGTGCTGCTGGGTCCCCAGGCGCGGGAGA GCTTCCGTTCATCCCGGCTGCAAACCCACAATTCCGTCATTGGCGTGCCCATCCGGGAGACTCCCATCCTGGATGACTATGACTGTGAGGAGGATGAGGA GCCACCTAGGCGGGCCAACCATGTCTCCCGCGAGGACGAGTTTGGCAGCCAGGTGACCCACACTCTGGACAGTCTGGGACATCCAGGGGAAGAGAAGGTG GACTTTGAGAAGAAAGCAGCAGCTGAGGCGACTCAGGAGACAGTGGAGTCCCTGATGCAGAAGTTCAAGGAGAGTTTCCGCGCTAACACGCCCATCGAGA TCGGTCAGCTGCAACCACCCCTGCGCAGCACATCGGCAGGGAAGAGGAAGCGGAGGAGCAAGTCTCGAGGAGGAATCAGCTTTGGGAGAGCCAAGGGGAC GTCGGGCTCAGAGGCAGACGATGAAACTCAGCTGACATTCTACACGGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTGCTGAAAAGCCCAGTGAAC AAGACAGCCCTGACACTGATTGCTGTGAGTTCCTGCATCCTGGCCATGGTGTGTGGCAGCCAGATGTCTTGTCCACTCACTGTGAAGGTGACTCTGCATG TGCCCGAGCACTTCATAGCAGATGGAAGCAGCTTCGTGGTGAGTGAAGGGAGCTACCTGGACATCTCCGACTGGTTAAACCCGGCCAAGCTTTCCCTGTA TTACCAGATCAATGCCACCTCCCCATGGGTGAGGGACCTCTGTGGACAAAGGACGACAGATGCCTGTGAGCAGCTCTGCGACCCAGAAACCGGAGAGTGC AGCTGTCATGAAGGCTATGCCCCTGACCCTGTTCACAGACACCTGTGTGTGCGCAGTGACTGGGGACAGAGTGAAGGACCTTGGCCCTACACGACACTTG AGAGGGGCTATGATCTGGTGACAGGGGAGCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAGCTTGGGCCAAGGCCTCTGGCTTCCTGTCAGCAAAAG CTTTGTGGTTCCGCCTGTGGAGCTGTCCATCAACCCCCTGGCCAGCTGCAAGACCGATGTGCTCGTCACGGAAGACCCTGCAGATGTCAGGGAAGAAGCG ATGCTGTCCACATACTTTGAAACCATCAATGACCTGCTGTCTTCCTTCGGGCCAGTTCGTGACTGCTCTCGGAACAATGGGGGCTGCACTCGCAACTTCA AGTGTGTGTCTGACCGGCAGGTGGATTCCTCGGGATGTGTGTGCCCTGAGGAGCTGAAACCCATGAAGGATGGCTCTGGCTGCTACGACCACTCCAAAGG CATTGACTGCTCTGATGGCTTTAATGGCGGCTGTGAGCAGCTGTGCCTGCAGCAGACGCTGCCCCTGCCCTACGATGCCACTTCGAGCACCATCTTCATG TTCTGCGGTTGCGTGGAGGAGTACAAACTGGCTCCTGATGGAAAATCCTGCTTAATGCTCTCAGATGTCTGCGAGGGCCCCAAGTGCCTCAAACCTGACT CCAAATTCAATGATACCCTCTTTGGAGAGATGCTACATGGTTACAACAACCGGACCCAGCATGTGAACCAAGGCCAAGTCTTCCAGATGACCTTTAGGGA GAACAACTTCATCAAGGACTTTCCCCAGCTGGCCGATGGGCTGTTGGTGATCCCGCTGCCGGTGGAGGAGCAGTGCCGGGGGGTCCTCTCCGAGCCCCTT CCAGACCTCCAACTGCTCACTGGAGATATCAGGTATGATGAGGCCATGGGTTACCCCATGGTGCAGCAGTGGCGGGTCCGGAGCAACCTCTACCGTGTGA AGCTCAGCACCATCACCCTCGCAGCAGGCTTCACTAATGTTCTCAAGATCCTGACCAAGGAGAGCAGTCGGGAGGAGCTGCTGTCCTTCATCCAGCACTA TGGCTCCCACTACATCGCAGAGGCCCTCTATGGCTCAGAGCTCACCTGCATCATCCACTTTCCCAGCAAGAAGGTCCAGCAGCAGCTGTGGCTCCAGTAT CAGAAAGAGACCACAGAGCTGGGCAGCAAGAAGGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCAGGTTTGCTGACAGCCCAGATGCTGTCAGATG ACCAGCTCATTTCAGGTGTGGAGATTCGCTGTGAGGAGAAGGGGCGCTGTCCATCTACCTGTCACCTTTGCCGCCGGCCAGGCAAGGAGCAGCTGAGCCC CACACCAGTGCTGCTGGAAATCAACCGTGTGGTGCCACTTTATACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAGTGCACTGATGAGTTCC TACTGGTGCTCAGGGAAAGGGGATGTGATCGATGACTGGTGCAGGTGTGACCTCAGCGCCTTTGATGCCAATGGGCTCCCCAACTGCAGCCCCCTTCTGC AGCCGGTGCTGCGGCTGTCCCCAACAGTGGAGCCCTCCAGTACTGTGGTCTCCTTGGAGTGGGTGGATGTTCAGCCAGCTATTGGGACCAAGGTCTCCGA CTATATTCTGCAGCATAAGAAAGTGGATGAATACACAGACACTGACCTGTACACAGGAGAATTCCTGAGTTTTGCTGATGACTTACTCTCTGGCCTGGGC ACATCTTGTGTAGCAGCTGGTCGAAGCCATGGAGAGGTCCCTGAAGTCAGTATCTACTCAGTCATCTTCAAGTGTCTGGAGCCCGACGGTCTCTACAAGT TCACTCTGTATGCTGTGGATACACGAGGGAGGCACTCAGAGCTAAGCACGGTGACCCTGAGGACGGCCTGTCCACTGGTAGATGACAACAAGGCAGAAGA AATAGCTGACAAGATCTACAATCTGTACAATGGGTACACAAGTGGAAAGGAGCAGCAGATGGCCTACAACACACTGATGGAGGTCTCAGCCTCGATGCTG TTCCGAGTCCAGCACCACTACAACTCTCACTATGAAAAGTTTGGCGACTTCGTCTGGAGAAGTGAGGATGAGCTGGGGCCCAGGAAGGCCCACCTGATTC TACGGCGACTGGAGAGGGTGAGTAGCCACTGCTCCAGCCTCCTGCGGAGTGCCTACATCCAGAGCCGCGTGGAAACAGTGCCCTATCTTTTCTGCCGCAG CGAGGAGGTCCGGCCTGCAGGCATGGTGTGGTATAGCATCCTCAAGGACACCAAAATCACGTGTGAGGAGAAGATGGTGTCAATGGCCCGAAACACGTAC GGGGAGTCCAAGGGCCGGTGAGGGAGGGTATTGCCCTCCGTGAGCACAGAGACTCTCCATGGGAGGGGGAGCAGTATTCTCCTGGATCCTGGGGCCTGGG TGGGCTGGGGGACAGCTGAGGATGGGCCTAGCAGATGAAGCTTGCCAGCAAGGCCAAAGCAAACGGTTTCTCCTGTGGATAGTGGACAGAGACCTTTGTA ACCAATGGAATTATTCATTTTTCTCTATCTTTTATTTTTTCAAAGATATTATTTGACTCTATCAAAAGTCTCTCCTTTTTAAACCTTTTCTTATGGATGG CTGTCAATCCCGAGGCAGAAGTTTTCAGGTGGAGACCAAGCGGCCTTTGCTCTTCTTCCTTCTTCCTGCCACACTCTGCTTTCTTCCTGCCATGGACCCC TGGAGGAGACCTATGGAGGGACAGTTTTGACCTGACCCCTAGAGGAGACAGTTTTGACCTCTTCAGCACCAGGAAGGAAGCTCTGAGGATGGTTGCAGTG AGGAAGCATGGGTCTTTAAGGACTTCTCTCTCTTTTTTGCTGGACATTATTGAGTTTGTGGGACCCTGCCTCTTCCTGCTACCTGTGGGTCTGCCCAGAG TCCCTGCAGGCCTGTCCATGCATTAAAAATTCCTATTGTCTCTC

refseq_ASTN2.F1 (forward primer)
refseq_ASTN2.R1 (reverse primer)
Single exon skipping

Frame 0

GSLRRPPSPQLPPLPPSPPPPPPPPPAAPQPLP PWPPPAPGSAPAPARGSGGGRGSASTRGRRHCCR CCCCSCSCCRRRRCWPAPPPLPRGSPTARAG*R PSRCPHCPPCGRATSAGAAPAPGPGLGPGPEPP PPPRPRALLALPAPPPSRASCSLCVTSCRGASRC RTTWTTPSCPSSPWRCLAQQRTSRWCTGDSSGW RMAPCTSTSP*AAPGSWPKPPPPLSRSPRRLLR SRCTSSTFL*WVASSRCCCCCWCSPWRCTPSDVG RSVAASPRRAQAQKPLMRSTTSHLCCWVPRRGR ASVHPGCKPTIPSLACPSGRLPSWMTMTVRRMR SHLGGPTMSPARTSLAAR*PTLWTVWDIQGKRRW TLRRKQQLRRLRRQWSP*CRSSRRVSALTRPSR SVSCNHPCAAHRQGRGSGGASLEEESALGEPRG RRAQRQTMKLS*HSTRSSTAVAAAAKVC*KAQ*T RQP*H*LL*VPASWPWCVAARCLVHSL*R*LCM CPSTS*QMEAASW*VKGATWTSPTG*TRPSFPC ITRSMPPPHG*GTSVDKGRQMPVSSSATQKPESA AVMKAMPLTLFTDTCVCAVTGDRVKDLGPTRHL RGAMIW*QGSKPLKRFSGLLSAWAKASGFLSAK ALWFRLWSCPSTPWPAARPMCSSRKTLQMSGKKR CCPHTLKPSMTCCLPSGQFVTALGTMGAALATS SVCLTGRWIPRDVCALRS*NP*RMALAATTTPK ALTALMALMAAVSSCACSRRCPCPTMPLRAPSSC SAVAWRSTNWLLMENPA*CSQMSARAPSASNLT PNSMIPSLERCYMVTTTGPSM*TKAKSSR*PLG RTTSSRTFPSWPMGCW*SRCRWRSSAGGSSPSPF QTSNCSLEISGMMRPWVTPWCSSGGSGATSTV* SSAPSPSQQASLMFSRS*PRRAVGRSCCPSSST MAPTTSQRPSMAQSSPASSTFPARRSSSSCGSSI RKRPQSWAARRSSSPCPSSPTSQVC*QPRCCQM TSSFQVWRFAVRRRGAVHLPVTFAAGQARSS*A PHQCCWKSTVWCHFIPSSKTMAQRRPSRVH**VP TGAQGKGM*SMTGAGVTSAPLMPMGSPTAAPFC SRCCGCPQQWSPPVLWSPWSGWMFSQLLGPRSP TIFCSIRKWMNTQTLTCTQENS*VLLMTYSLAWA HLV*QLVEAMERSLKSVSTQSSSSVWSPTVSTS SLCMLWIHEGGTQS*AR*P*GRPVHW*MTTRQK K*LTRSTICTMGTQVERSSRWPTTH*WRSQPRCC SESSTTTTLTMKSLATSSGEVRMSWGPGRPT*F YGDWRG*VATAPASCGVPTSRAAWKQCPIFSAA ARRSGLQAWCGIASSRTPKSRVRRRWCQWPETRT GSPRAGEGGYCPP*AQRLSMGGGAVFSWILGPG WAGGQLRMGLADEACQQGQSKRFLLWIVDRDLC NQWNYSFFSIFYFFKDII*LYQKSLLFKPFLMDG CQSRGRSFQVETKRPLLFFLLPATLCFLPAMDP WRRPMEGQF*PDP*RRQF*PLQHQEGSSEDGCS EEAWVFKDFSLFFAGHY*VCGTLPLPATCGSAQS PCRPVHALKIPIVS

Frame 1

VRFGVRRLRSCLLFLLRHRRHRRRRPQHRSPSR HGRRRRPAQPRPRLGAPGAAEALLPPGAAATAA AAAAVPAPAAAAAAAGRRHRRCLAGARQPVPAED RHGVHTARPAGERHRLERRPRRGRGWDRGRSRR RRRVPGLSWLCRHRRRVAPPALCA*RAAGAHRG AGRPGQHRAALLHPGDVWHSSGHLAGALETAVAG EWHLVLPRLHEQLRAAGPSHRPHSPGALGDC*G ADAHPPHFCDGWPHRAAAAAAGVHRGAVRPATL AEASPHPPEERKHRSHS*DPLHPICAAGSPGAGE LPFIPAANPQFRHWRAHPGDSHPG*L*L*GG*G AT*AGQPCLPRGRVWQPGDPHSGQSGTSRGREG GL*EESSS*GDSGDSGVPDAEVQGEFPR*HAHRD RSAATTPAQHIGREEEAEEQVSRRNQLWESQGD VGLRGRR*NSADILHGAVPQSPPQQRFAEKPSE QDSPDTDCCEFLHPGHGVWQPDVLSTHCEGDSAC ARALHSRWKQLRGE*RELPGHLRLVKPGQAFPV LPDQCHLPMGEGPLWTKDDRCL*AALRPRNRRV QLS*RLCP*PCSQTPVCAQ*LGTE*RTLALHDT* EGL*SGDRGASP*KDSQVYFQLGPRPLASCQQK LCGSACGAVHQPPGQLQDRCARHGRPCRCQGRS DAVHIL*NHQ*PAVFLRASS*LLSEQWGLHSQLQ VCV*PAGGFLGMCVP*GAETHEGWLWLLRPLQR H*LL*WL*WRL*AAVPAADAAPALRCHFEHHLH VLRLRGGVQTGS*WKILLNALRCLRGPQVPQT*L QIQ*YPLWRDATWLQQPDPACEPRPSLPDDL*G EQLHQGLSPAGRWAVGDPAAGGGAVPGGPLRAP SRPPTAHWRYQV**GHGLPHGAAVAGPEQPLPCE AQHHHPRSRLH*CSQDPDQGEQSGGAAVLHPAL WLPLHRRGPLWLRAHLHHPLSQQEGPAAAVAPV SERDHRAGQQEGAQVHALHHLPLRFADSPDAVR* PAHFRCGDSL*GEGALSIYLSPLPPARQGAAEP HTSAAGNQPCGATLYPHPRQWHKGGLQECTDEF LLVLRERGCDR*LVQV*PQRL*CQWAPQLQPPSA AGAAAVPNSGALQYCGLLGVGGCSASYWDQGLR LYSAA*ESG*IHRH*PVHRRIPEFC**LTLWPG HILCSSWSKPWRGP*SQYLLSHLQVSGARRSLQV HSVCCGYTREALRAKHGDPEDGLSTGR*QQGRR NS*QDLQSVQWVHKWKGAADGLQHTDGGLSLDA VPSPAPLQLSL*KVWRLRLEK*G*AGAQEGPPDS TATGEGE*PLLQPPAECLHPEPRGNSALSFLPQ RGGPACRHGVV*HPQGHQNHV*GEDGVNGPKHV RGVQGPVREGIALREHRDSPWEGEQYSPGSWGLG GLGDS*GWA*QMKLASKAKANGFSCG*WTETFV TNGIIHFSLSFIFSKILFDSIKSLSFLNLFLWM AVNPEAEVFRWRPSGLCSSSFFLPHSAFFLPWTP GGDLWRDSFDLTPRGDSFDLFSTRKEALRMVAV RKHGSLRTSLSFLLDIIEFVGPCLFLLPVGLPR VPAGLSMH*KFLLSL

Frame 2

FASASAVSAAASSSSFATAATAAAARSTAAPPA MAAAGARLSPGPGSGLRGRPRLCFHPGPPPLLP LLLLFLLLLPPPPLLAGATAAASREPDSPCRLK TVTVSTLPALRESDIGWSGARAGAGAGTGAGAAA AAASPGSPGSAGTAAESRLLLFVRNELPGRIAV QDDLDNTELPFFTLEMSGTAADISLVHWRQQWL ENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEE QMHILHISVMGGLIALLLLLLVFTVALYAQRRW QKRRRIPQKSASTEATHEIHYIPSVLLGPQARE SFRSSRLQTHNSVIGVPIRETPILDDYDCEEDEE PPRRANHVSREDEFGSQVTHTLDSLGHPGEEKV DFEKKAAAEATQETVESLMQKFKESFRANTPIE IGQLQPPLRSTSAGKRKRRSKSRGGISFGRAKGT SGSEADDETQLTFYTEQYRSRRRSKGLLKSPVN KTALTLIAVSSCILAMVCGSQMSCPLTVKVTLH VPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLY YQINATSPWVRDLCGQRTTDACEQLCDPETGEC SCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTL ERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKS FVVPPVELSINPLASCKTDVLVTEDPADVREEA MLSTYFETINDLLSSFGPVRDCSRNNGGCTRNF KCVSDRQVDSSGCVCPEELKPMKDGSGCYDHSKG IDCSDGFNGGCEQLCLQQTLPLPYDATSSTIFM FCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPD SKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRE NNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPL PDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRV KLSTITLAAGFTNVLKILTKESSREELLSFIQHY GSHYIAEALYGSELTCIIHFPSKKVQQQLWLQY QKETTELGSKKELKSMPFITYLSGLLTAQMLSD DQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSP TPVLLEINRVVPLYTLIQDNGTKEAFKSALMSS YWCSGKGDVIDDWCRCDLSAFDANGLPNCSPLL QPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSD YILQHKKVDEYTDTDLYTGEFLSFADDLLSGLG TSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYK FTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEE IADKIYNLYNGYTSGKEQQMAYNTLMEVSASML FRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLI LRRLERVSSHCSSLLRSAYIQSRVETVPYLFCRS EEVRPAGMVWYSILKDTKITCEEKMVSMARNTY GESKGR*GRVLPSVSTETLHGRGSSILLDPGAW VGWGTAEDGPSR*SLPARPKQTVSPVDSGQRPL* PMELFIFLYLLFFQRYYLTLSKVSPF*TFSYGW LSIPRQKFSGGDQAAFALLPSSCHTLLSSCHGP LEETYGGTVLT*PLEETVLTSSAPGRKL*GWLQ* GSMGL*GLLSLFCWTLLSLWDPASSCYLWVCPE SLQACPCIKNSYCL

Sequence NM_198186

GGTTCGCTTCGGCGTCCGCCGTCTCCGCAGCTGCCTCCTCTTCCTCCTTCGCCACCGCCGCCACCGCCGCCGCCGCCCGCAGCACCGCAGCCCCTCCCGC CATGGCCGCCGCCGGCGCCCGGCTCAGCCCCGGCCCCGGCTCGGGGCTCCGGGGGCGGCCGAGGCTCTGCTTCCACCCGGGGCCGCCGCCACTGCTGCCG CTGCTGCTGCTGTTCCTGCTCCTGCTGCCGCCGCCGCCGCTGCTGGCCGGCGCCACCGCCGCTGCCTCGCGGGAGCCCGACAGCCCGTGCCGGCTGAAGA CCGTCACGGTGTCCACACTGCCCGCCCTGCGGGAGAGCGACATCGGCTGGAGCGGCGCCCGCGCCGGGGCCGGGGCTGGGACCGGGGCCGGAGCCGCCGC CGCCGCCGCGTCCCCGGGCTCTCCTGGCTCTGCCGGCACCGCCGCCGAGTCGCGCCTCCTGCTCTTTGTGCGTAACGAGCTGCCGGGGCGCATCGCGGTG CAGGACGACCTGGACAACACCGAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCACAGCAGCGGACATCTCGCTGGTGCACTGGAGACAGCAGTGGCTGG AGAATGGCACCTTGTACTTCCACGTCTCCATGAGCAGCTCCGGGCAGCTGGCCCAAGCCACCGCCCCCACTCTCCAGGAGCCCTCGGAGATTGTTGAGGA GCAGATGCACATCCTCCACATTTCTGTGATGGGTGGCCTCATCGCGCTGCTGCTGCTGCTGCTGGTGTTCACCGTGGCGCTGTACGCCCAGCGACGTTGG CAGAAGCGTCGCCGCATCCCCCAGAAGAGCGCAAGCACAGAAGCCACTCATGAGATCCACTACATCCCATCTGTGCTGCTGGGTCCCCAGGCGCGGGAGA GCTTCCGTTCATCCCGGCTGCAAACCCACAATTCCGTCATTGGCGTGCCCATCCGGGAGACTCCCATCCTGGATGACTATGACTGTGAGGAGGATGAGGA GCCACCTAGGCGGGCCAACCATGTCTCCCGCGAGGACGAGTTTGGCAGCCAGGTGACCCACACTCTGGACAGTCTGGGACATCCAGGGGAAGAGAAGGTG GACTTTGAGAAGAAAGCAGCAGCTGAGGCGACTCAGGAGACAGTGGAGTCCCTGATGCAGAAGTTCAAGGAGAGTTTCCGCGCTAACACGCCCATCGAGA TCGGTCAGCTGCAACCACCCCTGCGCAGCACATCGGCAGGGAAGAGGAAGCGGAGGAGCAAGTCTCGAGGAGGAATCAGCTTTGGGAGAGCCAAGGGGAC GTCGGGCTCAGAGGCAGACGATGAAACTCAGCTGACATTCTACACGGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTGCTGAAAAGCCCAGTGAAC AAGACAGCCCTGACACTGATTGCTGTGAGTTCCTGCATCCTGGCCATGGTGTGTGGCAGCCAGATGTCTTGTCCACTCACTGTGAAGGTGACTCTGCATG TGCCCGAGCACTTCATAGCAGATGGAAGCAGCTTCGTGGTGAGTGAAGGGAGCTACCTGGACATCTCCGACTGGTTAAACCCGGCCAAGCTTTCCCTGTA TTACCAGATCAATGCCACCTCCCCATGGGTGAGGGACCTCTGTGGACAAAGGACGACAGATGCCTGTGAGCAGCTCTGCGACCCAGAAACCGGAGAGTGC AGCTGTCATGAAGGCTATGCCCCTGACCCTGTTCACAGACACCTGTGTGTGCGCAGTGACTGGGGACAGAGTGAAGGACCTTGGCCCTACACGACACTTG AGAGGGGCTATGATCTGGTGACAGGGGAGCAAGCCCCTGAAAAGATTCTCAGCTTGGGCCAAGGCCTCTGGCTTCCTGTCAGCAAAAGCTTTGTGGTTCC GCCTGTGGAGCTGTCCATCAACCCCCTGGCCAGCTGCAAGACCGATGTGCTCGTCACGGAAGACCCTGCAGATGTCAGGGAAGAAGCGATGCTGTCCACA TACTTTGAAACCATCAATGACCTGCTGTCTTCCTTCGGGCCAGTTCGTGACTGCTCTCGGAACAATGGGGGCTGCACTCGCAACTTCAAGTGTGTGTCTG ACCGGCAGGTGGATTCCTCGGGATGTGTGTGCCCTGAGGAGCTGAAACCCATGAAGGATGGCTCTGGCTGCTACGACCACTCCAAAGGCATTGACTGCTC TGATGGCTTTAATGGCGGCTGTGAGCAGCTGTGCCTGCAGCAGACGCTGCCCCTGCCCTACGATGCCACTTCGAGCACCATCTTCATGTTCTGCGGTTGC GTGGAGGAGTACAAACTGGCTCCTGATGGAAAATCCTGCTTAATGCTCTCAGATGTCTGCGAGGGCCCCAAGTGCCTCAAACCTGACTCCAAATTCAATG ATACCCTCTTTGGAGAGATGCTACATGGTTACAACAACCGGACCCAGCATGTGAACCAAGGCCAAGTCTTCCAGATGACCTTTAGGGAGAACAACTTCAT CAAGGACTTTCCCCAGCTGGCCGATGGGCTGTTGGTGATCCCGCTGCCGGTGGAGGAGCAGTGCCGGGGGGTCCTCTCCGAGCCCCTTCCAGACCTCCAA CTGCTCACTGGAGATATCAGGTATGATGAGGCCATGGGTTACCCCATGGTGCAGCAGTGGCGGGTCCGGAGCAACCTCTACCGTGTGAAGCTCAGCACCA TCACCCTCGCAGCAGGCTTCACTAATGTTCTCAAGATCCTGACCAAGGAGAGCAGTCGGGAGGAGCTGCTGTCCTTCATCCAGCACTATGGCTCCCACTA CATCGCAGAGGCCCTCTATGGCTCAGAGCTCACCTGCATCATCCACTTTCCCAGCAAGAAGGTCCAGCAGCAGCTGTGGCTCCAGTATCAGAAAGAGACC ACAGAGCTGGGCAGCAAGAAGGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCAGGTTTGCTGACAGCCCAGATGCTGTCAGATGACCAGCTCATTT CAGGTGTGGAGATTCGCTGTGAGGAGAAGGGGCGCTGTCCATCTACCTGTCACCTTTGCCGCCGGCCAGGCAAGGAGCAGCTGAGCCCCACACCAGTGCT GCTGGAAATCAACCGTGTGGTGCCACTTTATACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAGTGCACTGATGAGTTCCTACTGGTGCTCA GGGAAAGGGGATGTGATCGATGACTGGTGCAGGTGTGACCTCAGCGCCTTTGATGCCAATGGGCTCCCCAACTGCAGCCCCCTTCTGCAGCCGGTGCTGC GGCTGTCCCCAACAGTGGAGCCCTCCAGTACTGTGGTCTCCTTGGAGTGGGTGGATGTTCAGCCAGCTATTGGGACCAAGGTCTCCGACTATATTCTGCA GCATAAGAAAGTGGATGAATACACAGACACTGACCTGTACACAGGAGAATTCCTGAGTTTTGCTGATGACTTACTCTCTGGCCTGGGCACATCTTGTGTA GCAGCTGGTCGAAGCCATGGAGAGGTCCCTGAAGTCAGTATCTACTCAGTCATCTTCAAGTGTCTGGAGCCCGACGGTCTCTACAAGTTCACTCTGTATG CTGTGGATACACGAGGGAGGCACTCAGAGCTAAGCACGGTGACCCTGAGGACGGCCTGTCCACTGGTAGATGACAACAAGGCAGAAGAAATAGCTGACAA GATCTACAATCTGTACAATGGGTACACAAGTGGAAAGGAGCAGCAGATGGCCTACAACACACTGATGGAGGTCTCAGCCTCGATGCTGTTCCGAGTCCAG CACCACTACAACTCTCACTATGAAAAGTTTGGCGACTTCGTCTGGAGAAGTGAGGATGAGCTGGGGCCCAGGAAGGCCCACCTGATTCTACGGCGACTGG AGAGGGTGAGTAGCCACTGCTCCAGCCTCCTGCGGAGTGCCTACATCCAGAGCCGCGTGGAAACAGTGCCCTATCTTTTCTGCCGCAGCGAGGAGGTCCG GCCTGCAGGCATGGTGTGGTATAGCATCCTCAAGGACACCAAAATCACGTGTGAGGAGAAGATGGTGTCAATGGCCCGAAACACGTACGGGGAGTCCAAG GGCCGGTGAGGGAGGGTATTGCCCTCCGTGAGCACAGAGACTCTCCATGGGAGGGGGAGCAGTATTCTCCTGGATCCTGGGGCCTGGGTGGGCTGGGGGA CAGCTGAGGATGGGCCTAGCAGATGAAGCTTGCCAGCAAGGCCAAAGCAAACGGTTTCTCCTGTGGATAGTGGACAGAGACCTTTGTAACCAATGGAATT ATTCATTTTTCTCTATCTTTTATTTTTTCAAAGATATTATTTGACTCTATCAAAAGTCTCTCCTTTTTAAACCTTTTCTTATGGATGGCTGTCAATCCCG AGGCAGAAGTTTTCAGGTGGAGACCAAGCGGCCTTTGCTCTTCTTCCTTCTTCCTGCCACACTCTGCTTTCTTCCTGCCATGGACCCCTGGAGGAGACCT ATGGAGGGACAGTTTTGACCTGACCCCTAGAGGAGACAGTTTTGACCTCTTCAGCACCAGGAAGGAAGCTCTGAGGATGGTTGCAGTGAGGAAGCATGGG TCTTTAAGGACTTCTCTCTCTTTTTTGCTGGACATTATTGAGTTTGTGGGACCCTGCCTCTTCCTGCTACCTGTGGGTCTGCCCAGAGTCCCTGCAGGCC TGTCCATGCATTAAAAATTCCTATTGTCTCTC

refseq_ASTN2.F1 (forward primer)
refseq_ASTN2.R1 (reverse primer)
Single exon skipping

Frame 0

GSLRRPPSPQLPPLPPSPPPPPPPPPAAPQPLP PWPPPAPGSAPAPARGSGGGRGSASTRGRRHCCR CCCCSCSCCRRRRCWPAPPPLPRGSPTARAG*R PSRCPHCPPCGRATSAGAAPAPGPGLGPGPEPP PPPRPRALLALPAPPPSRASCSLCVTSCRGASRC RTTWTTPSCPSSPWRCLAQQRTSRWCTGDSSGW RMAPCTSTSP*AAPGSWPKPPPPLSRSPRRLLR SRCTSSTFL*WVASSRCCCCCWCSPWRCTPSDVG RSVAASPRRAQAQKPLMRSTTSHLCCWVPRRGR ASVHPGCKPTIPSLACPSGRLPSWMTMTVRRMR SHLGGPTMSPARTSLAAR*PTLWTVWDIQGKRRW TLRRKQQLRRLRRQWSP*CRSSRRVSALTRPSR SVSCNHPCAAHRQGRGSGGASLEEESALGEPRG RRAQRQTMKLS*HSTRSSTAVAAAAKVC*KAQ*T RQP*H*LL*VPASWPWCVAARCLVHSL*R*LCM CPSTS*QMEAASW*VKGATWTSPTG*TRPSFPC ITRSMPPPHG*GTSVDKGRQMPVSSSATQKPESA AVMKAMPLTLFTDTCVCAVTGDRVKDLGPTRHL RGAMIW*QGSKPLKRFSAWAKASGFLSAKALWF RLWSCPSTPWPAARPMCSSRKTLQMSGKKRCCPH TLKPSMTCCLPSGQFVTALGTMGAALATSSVCL TGRWIPRDVCALRS*NP*RMALAATTTPKALTA LMALMAAVSSCACSRRCPCPTMPLRAPSSCSAVA WRSTNWLLMENPA*CSQMSARAPSASNLTPNSM IPSLERCYMVTTTGPSM*TKAKSSR*PLGRTTS SRTFPSWPMGCW*SRCRWRSSAGGSSPSPFQTSN CSLEISGMMRPWVTPWCSSGGSGATSTV*SSAP SPSQQASLMFSRS*PRRAVGRSCCPSSSTMAPT TSQRPSMAQSSPASSTFPARRSSSSCGSSIRKRP QSWAARRSSSPCPSSPTSQVC*QPRCCQMTSSF QVWRFAVRRRGAVHLPVTFAAGQARSS*APHQC CWKSTVWCHFIPSSKTMAQRRPSRVH**VPTGAQ GKGM*SMTGAGVTSAPLMPMGSPTAAPFCSRCC GCPQQWSPPVLWSPWSGWMFSQLLGPRSPTIFC SIRKWMNTQTLTCTQENS*VLLMTYSLAWAHLV* QLVEAMERSLKSVSTQSSSSVWSPTVSTSSLCM LWIHEGGTQS*AR*P*GRPVHW*MTTRQKK*LT RSTICTMGTQVERSSRWPTTH*WRSQPRCCSESS TTTTLTMKSLATSSGEVRMSWGPGRPT*FYGDW RG*VATAPASCGVPTSRAAWKQCPIFSAAARRS GLQAWCGIASSRTPKSRVRRRWCQWPETRTGSPR AGEGGYCPP*AQRLSMGGGAVFSWILGPGWAGG QLRMGLADEACQQGQSKRFLLWIVDRDLCNQWN YSFFSIFYFFKDII*LYQKSLLFKPFLMDGCQSR GRSFQVETKRPLLFFLLPATLCFLPAMDPWRRP MEGQF*PDP*RRQF*PLQHQEGSSEDGCSEEAW VFKDFSLFFAGHY*VCGTLPLPATCGSAQSPCRP VHALKIPIVS

Frame 1

VRFGVRRLRSCLLFLLRHRRHRRRRPQHRSPSR HGRRRRPAQPRPRLGAPGAAEALLPPGAAATAA AAAAVPAPAAAAAAAGRRHRRCLAGARQPVPAED RHGVHTARPAGERHRLERRPRRGRGWDRGRSRR RRRVPGLSWLCRHRRRVAPPALCA*RAAGAHRG AGRPGQHRAALLHPGDVWHSSGHLAGALETAVAG EWHLVLPRLHEQLRAAGPSHRPHSPGALGDC*G ADAHPPHFCDGWPHRAAAAAAGVHRGAVRPATL AEASPHPPEERKHRSHS*DPLHPICAAGSPGAGE LPFIPAANPQFRHWRAHPGDSHPG*L*L*GG*G AT*AGQPCLPRGRVWQPGDPHSGQSGTSRGREG GL*EESSS*GDSGDSGVPDAEVQGEFPR*HAHRD RSAATTPAQHIGREEEAEEQVSRRNQLWESQGD VGLRGRR*NSADILHGAVPQSPPQQRFAEKPSE QDSPDTDCCEFLHPGHGVWQPDVLSTHCEGDSAC ARALHSRWKQLRGE*RELPGHLRLVKPGQAFPV LPDQCHLPMGEGPLWTKDDRCL*AALRPRNRRV QLS*RLCP*PCSQTPVCAQ*LGTE*RTLALHDT* EGL*SGDRGASP*KDSQLGPRPLASCQQKLCGS ACGAVHQPPGQLQDRCARHGRPCRCQGRSDAVH IL*NHQ*PAVFLRASS*LLSEQWGLHSQLQVCV* PAGGFLGMCVP*GAETHEGWLWLLRPLQRH*LL *WL*WRL*AAVPAADAAPALRCHFEHHLHVLRL RGGVQTGS*WKILLNALRCLRGPQVPQT*LQIQ* YPLWRDATWLQQPDPACEPRPSLPDDL*GEQLH QGLSPAGRWAVGDPAAGGGAVPGGPLRAPSRPP TAHWRYQV**GHGLPHGAAVAGPEQPLPCEAQHH HPRSRLH*CSQDPDQGEQSGGAAVLHPALWLPL HRRGPLWLRAHLHHPLSQQEGPAAAVAPVSERD HRAGQQEGAQVHALHHLPLRFADSPDAVR*PAHF RCGDSL*GEGALSIYLSPLPPARQGAAEPHTSA AGNQPCGATLYPHPRQWHKGGLQECTDEFLLVL RERGCDR*LVQV*PQRL*CQWAPQLQPPSAAGAA AVPNSGALQYCGLLGVGGCSASYWDQGLRLYSA A*ESG*IHRH*PVHRRIPEFC**LTLWPGHILC SSWSKPWRGP*SQYLLSHLQVSGARRSLQVHSVC CGYTREALRAKHGDPEDGLSTGR*QQGRRNS*Q DLQSVQWVHKWKGAADGLQHTDGGLSLDAVPSP APLQLSL*KVWRLRLEK*G*AGAQEGPPDSTATG EGE*PLLQPPAECLHPEPRGNSALSFLPQRGGP ACRHGVV*HPQGHQNHV*GEDGVNGPKHVRGVQ GPVREGIALREHRDSPWEGEQYSPGSWGLGGLGD S*GWA*QMKLASKAKANGFSCG*WTETFVTNGI IHFSLSFIFSKILFDSIKSLSFLNLFLWMAVNP EAEVFRWRPSGLCSSSFFLPHSAFFLPWTPGGDL WRDSFDLTPRGDSFDLFSTRKEALRMVAVRKHG SLRTSLSFLLDIIEFVGPCLFLLPVGLPRVPAG LSMH*KFLLSL

Frame 2

FASASAVSAAASSSSFATAATAAAARSTAAPPA MAAAGARLSPGPGSGLRGRPRLCFHPGPPPLLP LLLLFLLLLPPPPLLAGATAAASREPDSPCRLK TVTVSTLPALRESDIGWSGARAGAGAGTGAGAAA AAASPGSPGSAGTAAESRLLLFVRNELPGRIAV QDDLDNTELPFFTLEMSGTAADISLVHWRQQWL ENGTLYFHVSMSSSGQLAQATAPTLQEPSEIVEE QMHILHISVMGGLIALLLLLLVFTVALYAQRRW QKRRRIPQKSASTEATHEIHYIPSVLLGPQARE SFRSSRLQTHNSVIGVPIRETPILDDYDCEEDEE PPRRANHVSREDEFGSQVTHTLDSLGHPGEEKV DFEKKAAAEATQETVESLMQKFKESFRANTPIE IGQLQPPLRSTSAGKRKRRSKSRGGISFGRAKGT SGSEADDETQLTFYTEQYRSRRRSKGLLKSPVN KTALTLIAVSSCILAMVCGSQMSCPLTVKVTLH VPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLY YQINATSPWVRDLCGQRTTDACEQLCDPETGEC SCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTL ERGYDLVTGEQAPEKILSLGQGLWLPVSKSFVVP PVELSINPLASCKTDVLVTEDPADVREEAMLST YFETINDLLSSFGPVRDCSRNNGGCTRNFKCVS DRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCS DGFNGGCEQLCLQQTLPLPYDATSSTIFMFCGC VEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFN DTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFI KDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQ LLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLST ITLAAGFTNVLKILTKESSREELLSFIQHYGSHY IAEALYGSELTCIIHFPSKKVQQQLWLQYQKET TELGSKKELKSMPFITYLSGLLTAQMLSDDQLI SGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVL LEINRVVPLYTLIQDNGTKEAFKSALMSSYWCS GKGDVIDDWCRCDLSAFDANGLPNCSPLLQPVL RLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQ HKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCV AAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLY AVDTRGRHSELSTVTLRTACPLVDDNKAEEIADK IYNLYNGYTSGKEQQMAYNTLMEVSASMLFRVQ HHYNSHYEKFGDFVWRSEDELGPRKAHLILRRL ERVSSHCSSLLRSAYIQSRVETVPYLFCRSEEVR PAGMVWYSILKDTKITCEEKMVSMARNTYGESK GR*GRVLPSVSTETLHGRGSSILLDPGAWVGWG TAEDGPSR*SLPARPKQTVSPVDSGQRPL*PMEL FIFLYLLFFQRYYLTLSKVSPF*TFSYGWLSIP RQKFSGGDQAAFALLPSSCHTLLSSCHGPLEET YGGTVLT*PLEETVLTSSAPGRKL*GWLQ*GSMG L*GLLSLFCWTLLSLWDPASSCYLWVCPESLQA CPCIKNSYCL