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Sequences

Sequence NM_177999

CTTCCGGCGGGGGCGCGGCTTGGACTGAGGAGCGGACCCGGCCGGGCGCAGGGGCTGGACTGGCGGAGCAGGTGGGGTCCGCGGCCGCCGGAGCGTTCCG GTCGGCGTCTGGGCATCTCGGCCTCGGCAGAAAGCGCGACGGCCCTGCTGCGCGGGGCCCGCGGCGATGCCGTTCCTGCACGGCTTCCGGAGGATCATCT TCGAGTACCAGCCGCTGGTGGATGCGATTCTGGGCTCCCTGGGGATCCAGGACCCCGAGCGGCAGGAGTCTCTGGACCGGCCCAGTTATGTCGCCAGCGA GGAGAGCCGAATCCTTGTTCTCACTGAGCTGCTGGAGAGGAAAGCCCACTCTCCCTTTTACCAGGAAGGCGTGAGCAACGCCCTGCTCAAGATGGCTGAG CTGGGGCTGACGCGGGCGGCCGACGTTCTCTTGCGGCATGGGGCCAATCTCAACTTTGAAGACCCAGTCACCTACTACACGGCCTTGCACATCGCCGTCC TGCGGAACCAGCCGGACATGGTGGAGCTGCTGGTGCATCACGGGGCCGACGTTAATCGGAGGGACCGGGAAAAACTGCTCTGCTCCATGCTCTGGCCAGC AGCGACGGGGTGCAGATCCACAATACTGAGAACATTCGTCTCTTACTGGAAGGAGGGGCAGACGTCAAGGCCACCACCAAAGATGGGGACACAGTGTTCA CCTGCATCATCTTCCTGCTTGGTGAGACCGTGGGAGGGGACAAAGAGGAGGCCCAGATGATCAACCGCTTCTGCTTCCAAGTCACACGGCTGCTGCTGGC ACACGGGGCCGACCCCAGCGAGTGCCCAGCCCACGAGTCCCTCACCCACATCTGCCTGAAGAGCTTTAAACTGCACTTCCCTCTCCTGCGCTTCCTCCTG GAGTCCGGAGCCGCCTACAACTGCTCCCTGCACGGTGCGTCCTGCTGGTCTGGCTTTCACATCATCTTTGAGAGGCTCTGTTCCCACCCAGGCTGCACGG AAGACGAGAGCCATGCGGACCTCCTGCGCAAAGCTGAGACTGTCCTGGATCTCATGGTGACCAACTCTCAGAAACTCCAGCTGCCCGAAAACTTCGATAT CCACCCTGTGGGCAGCCTGGCGGAAAAGATCCAGGCCCTCCACTTCTCCTTGAGGCAGCTGGAGAGCTATCCCCCGCCCCTCAAGCACCTGTGCCGTGTG GCCATCCGGCTCTACCTTCAGCCGTGGCCTGTGGATGTGAAGGTCAAAGCCCTGCCTCTGCCCGACAGGCTGAAGTGGTACCTCCTTAGCGAGCACAGTG GCTCCGTGGAAGATGACATCTGATAGGTCTCAGGCTACAGGAACGGGGGACACGGGCAGCTCAGGTCAGCCTGTTGGTAGATGCTGGGACAGCATTAGAA GAGGGTCTTTTTGATCTGGGAGATGAGACGGGATCCTTCATGAGAAAAGCCAGCCAGGTAGAGCCTGAAGCTGTGATCACTCCAGTGGTGAAGGGTGCAG CTCTGGTTCTTCCAGAGAGACTGCCCCCTTCCCTCCAGCGGCACTCCAAGTCCCCCCCATGTTGGAAAGAGGAACAGGGCTTGGATTGATGTTTATACCC TCTGAATTGGAGGGCAGTGCCAGGGCATTTGCAGAAGCTGGGAAGGACCAAGCCTCAGCACCCCAGGGCTGCGGGGCTCTTGTTGGCCGCACGTGGCAGT GCAGCTGGAGAGGAAGCCTGGGGCACGTCCTGTGCGGTGCCACCAAGGCCCCACCCCTGCCCCTTTTCCTTCCAGGTTCTGTTACTCACAGAAGTGTTTC TGGTGGGCTCTGCGGGAGAAGACACTAAGGGCCCCACAGGCTTGTTTCATGGTGTTGCAAGCCAAGCTGGCCCCTGCCTGTTCCTGTAGCTCTGGGGCCA GGTCAGCCGTGGCCCCACCTACCAGAACTGTGCTCTGCTGGCAGCTGTGCTTTTGCCAAACCCAGGGCCTCAGCGACCATGACTTCTGCTAAAAAGCAAT CATGAGTGCGTGGCTGGGGACAGACCTCTCTGAAGTGTCTCCTGTTGTGAGGGGCTCACTTTGGAGCCACTGAAGAGCCGACTGTGCCACCCACTTGCGA GCCTCAGTCCCTTCCAAGCTGCACTGGTCATTGGGAATTGTGTAAACACCTCTGGGGATAAGCTGAGGTGACTAAATGATATATTTATATTATAAGATTA AACTGACTTTCTTGGCCTTAACAAGGTCCTTATATAAAATTTCCACCCCGCCCCTCCATAGTTCAGGATAGAGGCTCCCACAGCAGCCGGTAAGGAAGTG GGTGGCAGGTGTGAGGGGATGCTGTGTTTGGGGGGTTGTTTCCAAGTCCCTGCAAAGAAGCACCACTCTAGACTGGCAAGGGCCTCCACAGTGTGGAGGT CCCATTTTATTATTTTTTTTTTTCGAGACACTCTGTCACCCAGGCTGGAGTGCAATGGTGCAATCTCGGCTCACTGCGACCTCTGCCTCCCAGGTTCAAG CAGTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGCATTATAGGTGCCCACCACCATGCCTGGCTGATTTTTGTATTTTTAGTAGAGACGGGGTTTCGCCAT GTTGGTCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATTCGCCTGCCTCGGCCTTTCAAAGTGCTGGGATTACAGACATGAGCCACTGTGCCCGGCCCC CATTTTATTTTAGTTTTATTTTGAGACAGAGTCTCGCTCTGTCACCTAGGCTGGAGTGCAGTGGCGCAATCTCACCTCACTGCAAGCTCTGCCTCCCGGG CTCACCCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCATCACGCCTGGCTAATTTTTTTTTTTTTTGTATTTTAAGTAGAG ACGGGGTTTCACCTGTTAGCCAGGATGGTCTCAATCTCCTGACCTCGTGATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACC GCGCCCAGCCCCATTTTATTTTTTACTTTAAATGCCTGCCAAGGAGCAGCCTCAAGCCCAGAGAAGACTGAGGCTACGGGGAACTTCCCTTGCTGCAGGC TGTTGTAACACTTTAAGGGCCCCAGGTCTCCACTGCCAAGCAGGACTTGGCACATGAGCACCCACCCCACAGCACCATCAGGCAGCACCCATGGGCTCCA CCAGCCCAGCTCTGTCCCTCCCCTAGGTAAAGATCACACTGAAGTCTTCAAGTCCCCAGCAACCAAGTTGGGGGTGGTTTCCTTCCTCTTCAGGCAGCCA GGCCCATGGCTGGAAGGCCGGGCTGGAGGCCCAGGGAATCGGTTACTGATGTGGCCACCCTATTCCCACATGGGGAGCACCAGGAGCCCTGGAGCCCCTT CCACCCAATCCCATCTCGCAGGAGAGCAGGGTCTGGGCTCCTGCCTCACCGCTGGTTCAGCAGCACCCTCCCCGCCGGGTCCCACCTTGCCTTTTGAAGA AGAGCCCGCATAATGAGTGGACGGCAGACAGCTATATTTAGTGGTGCCTCGACACTCACGAACCGCCAGCGTGGCGCCTGGATCTTGCCCAGCTGCCAGC TCCCCCCACCAGGACTGTGGTTCCTCAGTTTCTCCTGCCAGCCCCGGCTCATCTCAGGGCAAAGCTATAGACATGGTAGATCTCATCGGGGAGGTTCTCC TGCCTCTCCTCGGCCAGGAGGCTGAGGCCTGCACTGCAGATGATCCTGCGGACCACGTCAAGGTCCCGGCACACGCTGCTGTCCACGTCGTCCAGAATCA CGCCCTCCTGGGCCATGTTGTCTTTGATGACGATGATGCCGTTGGGGCGGAGGCTGCCCTTGCAGCGCCGCAGGAACTCGGCCAGGTGCTGATCGGTGAG GTGGCCTGGGGAGGGCAGAGGCAAGGTTACCAGGGCACCATGCAAGCGGATGTCGGCACTGGATGGGATGTACTTCTCAGCCCCGCTGAGCCCCTCATTT GTGCCCAGCATTTTGTGTGCACACATGGCCTCATTGGCTCTCAGTCCCGAGAGATTTACTAAACCCCAATTCATAGAATAGGAAACTGTGGCTCAGAGGT CATCTACCTAAAGACACATTAAGCCAGAATTTGACTTGACGGGATGGCTTACAAGTCCTGCCTGATACCTACACACCATGATGCCCCACCCCTGCCAGGC TTGGATCGGAGGAGTGAACCTGGGCTAAGAGTTTGGCATTGCAGGAGCTCCCAGAGAGACTGCCTTCCCTACACTAGAAGTCCCCACAAAGGCTTCTGAG ACCACAAAATACAAGGCCAGACCTAGAGTGTCCACAGCAGGCCTGTGCTGCCCGCCTCAGGAGGTGGCCGACTGCTGCTAGCGGCCTGCCGCCACGTCAG CAGCCCAGGGCACAGCGCTCGAGGTGTGCTGTTAGGCTAAGAGGGTGCAGGGCTAGACACGAAGCTTAAACTATTCATCTTATTAAAAATAAAACCCTTA TAAAACTCC

refseq_ASB6.F1 (forward primer)
refseq_ASB6.R1 (reverse primer)
Single exon skipping

Frame 0

LPAGARLGLRSGPGRAQGLDWRSRWGPRPPERS GRRLGISASAESATALLRGARGDAVPARLPEDHL RVPAAGGCDSGLPGDPGPRAAGVSGPAQLCRQR GEPNPCSH*AAGEESPLSLLPGRREQRPAQDG* AGADAGGRRSLAAWGQSQL*RPSHLLHGLAHRRP AEPAGHGGAAGASRGRR*SEGPGKTALLHALAS SDGVQIHNTENIRLLLEGGADVKATTKDGDTVF TCIIFLLGETVGGDKEEAQMINRFCFQVTRLLLA HGADPSECPAHESLTHICLKSFKLHFPLLRFLL ESGAAYNCSLHGASCWSGFHIIFERLCSHPGCT EDESHADLLRKAETVLDLMVTNSQKLQLPENFDI HPVGSLAEKIQALHFSLRQLESYPPPLKHLCRV AIRLYLQPWPVDVKVKALPLPDRLKWYLLSEHS GSVEDDI**VSGYRNGGHGQLRSACW*MLGQH*K RVFLIWEMRRDPS*EKPAR*SLKL*SLQW*RVQ LWFFQRDCPLPSSGTPSPPHVGKRNRAWIDVYT L*IGGQCQGICRSWEGPSLSTPGLRGSCWPHVAV QLERKPGARPVRCHQGPTPAPFPSRFCYSQKCF WWALREKTLRAPQACFMVLQAKLAPACSCSSGA RSAVAPPTRTVLCWQLCFCQTQGLSDHDFC*KAI MSAWLGTDLSEVSPVVRGSLWSH*RADCATHLR ASVPSKLHWSLGIV*TPLGIS*GD*MIYLYYKI KLTFLALTRSLYKISTPPLHSSG*RLPQQPVRKW VAGVRGCCVWGVVSKSLQRSTTLDWQGPPQCGG PILLFFFFETLCHPGWSAMVQSRLTATSASQVQ AVLLPQPPE*LAL*VPTTMPG*FLYF**RRGFAM LVRLVSNS*PQVIRLPRPFKVLGLQT*ATVPGP HFILVLF*DRVSLCHLGWSAVAQSHLTASSASR AHPILLPQPPE*LGLQAPAITPG*FFFFLYFK*R RGFTC*PGWSQSPDLVICPPRPPKVLGLQA*AT APSPILFFTLNACQGAASSPEKTEATGNFPCCR LL*HFKGPRSPLPSRTWHMSTHPTAPSGSTHGLH QPSSVPPLGKDHTEVFKSPATKLGVVSFLFRQP GPWLEGRAGGPGNRLLMWPPYSHMGSTRSPGAP STQSHLAGEQGLGSCLTAGSAAPSPPGPTLPFEE EPA**VDGRQLYLVVPRHSRTASVAPGSCPAAS SPHQDCGSSVSPASPGSSQGKAIDMVDLIGEVL LPLLGQEAEACTADDPADHVKVPAHAAVHVVQNH ALLGHVVFDDDDAVGAEAALAAPQELGQVLIGE VAWGGQRQGYQGTMQADVGTGWDVLLSPAEPLI CAQHFVCTHGLIGSQSREIY*TPIHRIGNCGSEV IYLKTH*ARI*LDGMAYKSCLIPTHHDAPPLPG LDRRSEPGLRVWHCRSSQRDCLPYTRSPHKGF* DHKIQGQT*SVHSRPVLPASGGGRLLLAACRHVS SPGHSARGVLLG*EGAGLDTKLKLFILLKIKPL *NS

Frame 1

FRRGRGLD*GADPAGRRGWTGGAGGVRGRRSVP VGVWASRPRQKARRPCCAGPAAMPFLHGFRRII FEYQPLVDAILGSLGIQDPERQESLDRPSYVASE ESRILVLTELLERKAHSPFYQEGVSNALLKMAE LGLTRAADVLLRHGANLNFEDPVTYYTALHIAV LRNQPDMVELLVHHGADVNRRDREKLLCSMLWPA ATGCRSTILRTFVSYWKEGQTSRPPPKMGTQCS PASSSCLVRPWEGTKRRPR*STASASKSHGCCW HTGPTPASAQPTSPSPTSA*RALNCTSLSCASSW SPEPPTTAPCTVRPAGLAFTSSLRGSVPTQAAR KTRAMRTSCAKLRLSWISW*PTLRNSSCPKTSI STLWAAWRKRSRPSTSP*GSWRAIPRPSSTCAVW PSGSTFSRGLWM*RSKPCLCPTG*SGTSLASTV APWKMTSDRSQATGTGDTGSSGQPVGRCWDSIR RGSF*SGR*DGILHEKSQPGRA*SCDHSSGEGCS SGSSRETAPFPPAALQVPPMLERGTGLGLMFIP SELEGSARAFAEAGKDQASAPQGCGALVGRTWQ CSWRGSLGHVLCGATKAPPLPLFLPGSVTHRSVS GGLCGRRH*GPHRLVSWCCKPSWPLPVPVALGP GQPWPHLPELCSAGSCAFAKPRASATMTSAKKQ S*VRGWGQTSLKCLLL*GAHFGATEEPTVPPTCE PQSLPSCTGHWELCKHLWG*AEVTK*YIYIIRL N*LSWP*QGPYIKFPPRPSIVQDRGSHSSR*GS GWQV*GDAVFGGLFPSPCKEAPL*TGKGLHSVEV PFYYFFFSRHSVTQAGVQWCNLGSLRPLPPRFK QFSCLSLLSSWHYRCPPPCLADFCIFSRDGVSP CWSGWSQTPDLR*FACLGLSKCWDYRHEPLCPAP ILF*FYFETESRSVT*AGVQWRNLTSLQALPPG LTPFSCLSLLSSWDYRRPPSRLANFFFFCILSR DGVSPVSQDGLNLLTS*SARLGLPKCWDYRREPP RPAPFYFLL*MPAKEQPQAQRRLRLRGTSLAAG CCNTLRAPGLHCQAGLGT*APTPQHHQAAPMGS TSPALSLP*VKITLKSSSPQQPSWGWFPSSSGSQ AHGWKAGLEAQGIGY*CGHPIPTWGAPGALEPL PPNPISQESRVWAPASPLVQQHPPRRVPPCLLK KSPHNEWTADSYI*WCLDTHEPPAWRLDLAQLPA PPTRTVVPQFLLPAPAHLRAKL*TW*ISSGRFS CLSSARRLRPALQMILRTTSRSRHTLLSTSSRI TPSWAMLSLMTMMPLGRRLPLQRRRNSARC*SVR WPGEGRGKVTRAPCKRMSALDGMYFSAPLSPSF VPSILCAHMASLALSPERFTKPQFIE*ETVAQR SST*RHIKPEFDLTGWLTSPA*YLHTMMPHPCQA WIGGVNLG*EFGIAGAPRETAFPTLEVPTKASE TTKYKARPRVSTAGLCCPPQEVADCC*RPAATS AAQGTALEVCC*AKRVQG*TRSLNYSSY*K*NPY KT

Frame 2

SGGGAAWTEERTRPGAGAGLAEQVGSAAAGAFR SASGHLGLGRKRDGPAARGPRRCRSCTASGGSS SSTSRWWMRFWAPWGSRTPSGRSLWTGPVMSPA RRAESLFSLSCWRGKPTLPFTRKA*ATPCSRWLS WG*RGRPTFSCGMGPISTLKTQSPTTRPCTSPS CGTSRTWWSCWCITGPTLIGGTGKNCSAPCSGQ QRRGADPQY*EHSSLTGRRGRRQGHHQRWGHSVH LHHLPAW*DRGRGQRGGPDDQPLLLPSHTAAAG TRGRPQRVPSPRVPHPHLPEEL*TALPSPALPP GVRSRLQLLPARCVLLVWLSHHL*EALFPPRLHG RREPCGPPAQS*DCPGSHGDQLSETPAARKLRY PPCGQPGGKDPGPPLLLEAAGELSPAPQAPVPC GHPALPSAVACGCEGQSPASARQAEVVPP*RAQW LRGR*HLIGLRLQERGTRAAQVSLLVDAGTALE EGLFDLGDETGSFMRKASQVEPEAVITPVVKGA ALVLPERLPPSLQRHSKSPPCWKEEQGLD*CLYP LNWRAVPGHLQKLGRTKPQHPRAAGLLLAARGS AAGEEAWGTSCAVPPRPHPCPFSFQVLLLTEVF LVGSAGEDTKGPTGLFHGVASQAGPCLFL*LWGQ VSRGPTYQNCALLAAVLLPNPGPQRP*LLLKSN HECVAGDRPL*SVSCCEGLTLEPLKSRLCHPLA SLSPFQAALVIGNCVNTSGDKLR*LNDIFIL*D* TDFLGLNKVLI*NFHPAPP*FRIEAPTAAGKEV GGRCEGMLCLGGCFQVPAKKHHSRLARASTVWR SHFIIFFFRDTLSPRLECNGAISAHCDLCLPGSS SSPASAS*VAGIIGAHHHAWLIFVFLVETGFRH VGQAGLKLLTSGDSPASAFQSAGITDMSHCARP PFYFSFILRQSLALSPRLECSGAISPHCKLCLPG SPHSPASAS*VAGTTGARHHAWLIFFFFVF*VE TGFHLLARMVSIS*PRDLPASASQSAGITGVSH RAQPHFIFYFKCLPRSSLKPRED*GYGELPLLQA VVTL*GPQVSTAKQDLAHEHPPHSTIRQHPWAP PAQLCPSPR*RSH*SLQVPSNQVGGGFLPLQAA RPMAGRPGWRPRESVTDVATLFPHGEHQEPWSPF HPIPSRRRAGSGLLPHRWFSSTLPAGSHLAF*R RARIMSGRQTAIFSGASTLTNRQRGAWILPSCQ LPPPGLWFLSFSCQPRLISGQSYRHGRSHRGGSP ASPRPGG*GLHCR*SCGPRQGPGTRCCPRRPES RPPGPCCL**R*CRWGGGCPCSAAGTRPGADR* GGLGRAEARLPGHHASGCRHWMGCTSQPR*APHL CPAFCVHTWPHWLSVPRDLLNPNS*NRKLWLRG HLPKDTLSQNLT*RDGLQVLPDTYTP*CPTPAR LGSEE*TWAKSLALQELPERLPSLH*KSPQRLLR PQNTRPDLECPQQACAARLRRWPTAASGLPPRQ QPRAQRSRCAVRLRGCRARHEA*TIHLIKNKTL IKL

Sequence NM_017873

CTTCCGGCGGGGGCGCGGCTTGGACTGAGGAGCGGACCCGGCCGGGCGCAGGGGCTGGACTGGCGGAGCAGGTGGGGTCCGCGGCCGCCGGAGCGTTCCG GTCGGCGTCTGGGCATCTCGGCCTCGGCAGAAAGCGCGACGGCCCTGCTGCGCGGGGCCCGCGGCGATGCCGTTCCTGCACGGCTTCCGGAGGATCATCT TCGAGTACCAGCCGCTGGTGGATGCGATTCTGGGCTCCCTGGGGATCCAGGACCCCGAGCGGCAGGAGTCTCTGGACCGGCCCAGTTATGTCGCCAGCGA GGAGAGCCGAATCCTTGTTCTCACTGAGCTGCTGGAGAGGAAAGCCCACTCTCCCTTTTACCAGGAAGGCGTGAGCAACGCCCTGCTCAAGATGGCTGAG CTGGGGCTGACGCGGGCGGCCGACGTTCTCTTGCGGCATGGGGCCAATCTCAACTTTGAAGACCCAGTCACCTACTACACGGCCTTGCACATCGCCGTCC TGCGGAACCAGCCGGACATGGTGGAGCTGCTGGTGCATCACGGGGCCGACGTTAATCGGAGGGACCGGATCCACGAGAGTAGCCCCTTGGACCTGGCCAG CGAGGAGCCTGAGCGCCTGCCCTGCCTGCAGCGCCTCCTGGACCTTGGAGCTGATGTCAATGCCGCTGACAAGCATGGAAAAACTGCTCTGCTCCATGCT CTGGCCAGCAGCGACGGGGTGCAGATCCACAATACTGAGAACATTCGTCTCTTACTGGAAGGAGGGGCAGACGTCAAGGCCACCACCAAAGATGGGGACA CAGTGTTCACCTGCATCATCTTCCTGCTTGGTGAGACCGTGGGAGGGGACAAAGAGGAGGCCCAGATGATCAACCGCTTCTGCTTCCAAGTCACACGGCT GCTGCTGGCACACGGGGCCGACCCCAGCGAGTGCCCAGCCCACGAGTCCCTCACCCACATCTGCCTGAAGAGCTTTAAACTGCACTTCCCTCTCCTGCGC TTCCTCCTGGAGTCCGGAGCCGCCTACAACTGCTCCCTGCACGGTGCGTCCTGCTGGTCTGGCTTTCACATCATCTTTGAGAGGCTCTGTTCCCACCCAG GCTGCACGGAAGACGAGAGCCATGCGGACCTCCTGCGCAAAGCTGAGACTGTCCTGGATCTCATGGTGACCAACTCTCAGAAACTCCAGCTGCCCGAAAA CTTCGATATCCACCCTGTGGGCAGCCTGGCGGAAAAGATCCAGGCCCTCCACTTCTCCTTGAGGCAGCTGGAGAGCTATCCCCCGCCCCTCAAGCACCTG TGCCGTGTGGCCATCCGGCTCTACCTTCAGCCGTGGCCTGTGGATGTGAAGGTCAAAGCCCTGCCTCTGCCCGACAGGCTGAAGTGGTACCTCCTTAGCG AGCACAGTGGCTCCGTGGAAGATGACATCTGATAGGTCTCAGGCTACAGGAACGGGGGACACGGGCAGCTCAGGTCAGCCTGTTGGTAGATGCTGGGACA GCATTAGAAGAGGGTCTTTTTGATCTGGGAGATGAGACGGGATCCTTCATGAGAAAAGCCAGCCAGGTAGAGCCTGAAGCTGTGATCACTCCAGTGGTGA AGGGTGCAGCTCTGGTTCTTCCAGAGAGACTGCCCCCTTCCCTCCAGCGGCACTCCAAGTCCCCCCCATGTTGGAAAGAGGAACAGGGCTTGGATTGATG TTTATACCCTCTGAATTGGAGGGCAGTGCCAGGGCATTTGCAGAAGCTGGGAAGGACCAAGCCTCAGCACCCCAGGGCTGCGGGGCTCTTGTTGGCCGCA CGTGGCAGTGCAGCTGGAGAGGAAGCCTGGGGCACGTCCTGTGCGGTGCCACCAAGGCCCCACCCCTGCCCCTTTTCCTTCCAGGTTCTGTTACTCACAG AAGTGTTTCTGGTGGGCTCTGCGGGAGAAGACACTAAGGGCCCCACAGGCTTGTTTCATGGTGTTGCAAGCCAAGCTGGCCCCTGCCTGTTCCTGTAGCT CTGGGGCCAGGTCAGCCGTGGCCCCACCTACCAGAACTGTGCTCTGCTGGCAGCTGTGCTTTTGCCAAACCCAGGGCCTCAGCGACCATGACTTCTGCTA AAAAGCAATCATGAGTGCGTGGCTGGGGACAGACCTCTCTGAAGTGTCTCCTGTTGTGAGGGGCTCACTTTGGAGCCACTGAAGAGCCGACTGTGCCACC CACTTGCGAGCCTCAGTCCCTTCCAAGCTGCACTGGTCATTGGGAATTGTGTAAACACCTCTGGGGATAAGCTGAGGTGACTAAATGATATATTTATATT ATAAGATTAAACTGACTTTCTTGGCCTTAACAAGGTCCTTATATAAAATTTCCACCCCGCCCCTCCATAGTTCAGGATAGAGGCTCCCACAGCAGCCGGT AAGGAAGTGGGTGGCAGGTGTGAGGGGATGCTGTGTTTGGGGGGTTGTTTCCAAGTCCCTGCAAAGAAGCACCACTCTAGACTGGCAAGGGCCTCCACAG TGTGGAGGTCCCATTTTATTATTTTTTTTTTTCGAGACACTCTGTCACCCAGGCTGGAGTGCAATGGTGCAATCTCGGCTCACTGCGACCTCTGCCTCCC AGGTTCAAGCAGTTCTCCTGCCTCAGCCTCCTGAGTAGCTGGCATTATAGGTGCCCACCACCATGCCTGGCTGATTTTTGTATTTTTAGTAGAGACGGGG TTTCGCCATGTTGGTCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATTCGCCTGCCTCGGCCTTTCAAAGTGCTGGGATTACAGACATGAGCCACTGTG CCCGGCCCCCATTTTATTTTAGTTTTATTTTGAGACAGAGTCTCGCTCTGTCACCTAGGCTGGAGTGCAGTGGCGCAATCTCACCTCACTGCAAGCTCTG CCTCCCGGGCTCACCCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCATCACGCCTGGCTAATTTTTTTTTTTTTTGTATTT TAAGTAGAGACGGGGTTTCACCTGTTAGCCAGGATGGTCTCAATCTCCTGACCTCGTGATCTGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCG TGAGCCACCGCGCCCAGCCCCATTTTATTTTTTACTTTAAATGCCTGCCAAGGAGCAGCCTCAAGCCCAGAGAAGACTGAGGCTACGGGGAACTTCCCTT GCTGCAGGCTGTTGTAACACTTTAAGGGCCCCAGGTCTCCACTGCCAAGCAGGACTTGGCACATGAGCACCCACCCCACAGCACCATCAGGCAGCACCCA TGGGCTCCACCAGCCCAGCTCTGTCCCTCCCCTAGGTAAAGATCACACTGAAGTCTTCAAGTCCCCAGCAACCAAGTTGGGGGTGGTTTCCTTCCTCTTC AGGCAGCCAGGCCCATGGCTGGAAGGCCGGGCTGGAGGCCCAGGGAATCGGTTACTGATGTGGCCACCCTATTCCCACATGGGGAGCACCAGGAGCCCTG GAGCCCCTTCCACCCAATCCCATCTCGCAGGAGAGCAGGGTCTGGGCTCCTGCCTCACCGCTGGTTCAGCAGCACCCTCCCCGCCGGGTCCCACCTTGCC TTTTGAAGAAGAGCCCGCATAATGAGTGGACGGCAGACAGCTATATTTAGTGGTGCCTCGACACTCACGAACCGCCAGCGTGGCGCCTGGATCTTGCCCA GCTGCCAGCTCCCCCCACCAGGACTGTGGTTCCTCAGTTTCTCCTGCCAGCCCCGGCTCATCTCAGGGCAAAGCTATAGACATGGTAGATCTCATCGGGG AGGTTCTCCTGCCTCTCCTCGGCCAGGAGGCTGAGGCCTGCACTGCAGATGATCCTGCGGACCACGTCAAGGTCCCGGCACACGCTGCTGTCCACGTCGT CCAGAATCACGCCCTCCTGGGCCATGTTGTCTTTGATGACGATGATGCCGTTGGGGCGGAGGCTGCCCTTGCAGCGCCGCAGGAACTCGGCCAGGTGCTG ATCGGTGAGGTGGCCTGGGGAGGGCAGAGGCAAGGTTACCAGGGCACCATGCAAGCGGATGTCGGCACTGGATGGGATGTACTTCTCAGCCCCGCTGAGC CCCTCATTTGTGCCCAGCATTTTGTGTGCACACATGGCCTCATTGGCTCTCAGTCCCGAGAGATTTACTAAACCCCAATTCATAGAATAGGAAACTGTGG CTCAGAGGTCATCTACCTAAAGACACATTAAGCCAGAATTTGACTTGACGGGATGGCTTACAAGTCCTGCCTGATACCTACACACCATGATGCCCCACCC CTGCCAGGCTTGGATCGGAGGAGTGAACCTGGGCTAAGAGTTTGGCATTGCAGGAGCTCCCAGAGAGACTGCCTTCCCTACACTAGAAGTCCCCACAAAG GCTTCTGAGACCACAAAATACAAGGCCAGACCTAGAGTGTCCACAGCAGGCCTGTGCTGCCCGCCTCAGGAGGTGGCCGACTGCTGCTAGCGGCCTGCCG CCACGTCAGCAGCCCAGGGCACAGCGCTCGAGGTGTGCTGTTAGGCTAAGAGGGTGCAGGGCTAGACACGAAGCTTAAACTATTCATCTTATTAAAAATA AAACCCTTATAAAACTCC

refseq_ASB6.F1 (forward primer)
refseq_ASB6.R1 (reverse primer)
Single exon skipping

Frame 0

LPAGARLGLRSGPGRAQGLDWRSRWGPRPPERS GRRLGISASAESATALLRGARGDAVPARLPEDHL RVPAAGGCDSGLPGDPGPRAAGVSGPAQLCRQR GEPNPCSH*AAGEESPLSLLPGRREQRPAQDG* AGADAGGRRSLAAWGQSQL*RPSHLLHGLAHRRP AEPAGHGGAAGASRGRR*SEGPDPRE*PLGPGQ RGA*APALPAAPPGPWS*CQCR*QAWKNCSAPC SGQQRRGADPQY*EHSSLTGRRGRRQGHHQRWGH SVHLHHLPAW*DRGRGQRGGPDDQPLLLPSHTA AAGTRGRPQRVPSPRVPHPHLPEEL*TALPSPA LPPGVRSRLQLLPARCVLLVWLSHHL*EALFPPR LHGRREPCGPPAQS*DCPGSHGDQLSETPAARK LRYPPCGQPGGKDPGPPLLLEAAGELSPAPQAP VPCGHPALPSAVACGCEGQSPASARQAEVVPP*R AQWLRGR*HLIGLRLQERGTRAAQVSLLVDAGT ALEEGLFDLGDETGSFMRKASQVEPEAVITPVV KGAALVLPERLPPSLQRHSKSPPCWKEEQGLD*C LYPLNWRAVPGHLQKLGRTKPQHPRAAGLLLAA RGSAAGEEAWGTSCAVPPRPHPCPFSFQVLLLT EVFLVGSAGEDTKGPTGLFHGVASQAGPCLFL*L WGQVSRGPTYQNCALLAAVLLPNPGPQRP*LLL KSNHECVAGDRPL*SVSCCEGLTLEPLKSRLCH PLASLSPFQAALVIGNCVNTSGDKLR*LNDIFIL *D*TDFLGLNKVLI*NFHPAPP*FRIEAPTAAG KEVGGRCEGMLCLGGCFQVPAKKHHSRLARAST VWRSHFIIFFFRDTLSPRLECNGAISAHCDLCLP GSSSSPASAS*VAGIIGAHHHAWLIFVFLVETG FRHVGQAGLKLLTSGDSPASAFQSAGITDMSHC ARPPFYFSFILRQSLALSPRLECSGAISPHCKLC LPGSPHSPASAS*VAGTTGARHHAWLIFFFFVF *VETGFHLLARMVSIS*PRDLPASASQSAGITG VSHRAQPHFIFYFKCLPRSSLKPRED*GYGELPL LQAVVTL*GPQVSTAKQDLAHEHPPHSTIRQHP WAPPAQLCPSPR*RSH*SLQVPSNQVGGGFLPL QAARPMAGRPGWRPRESVTDVATLFPHGEHQEPW SPFHPIPSRRRAGSGLLPHRWFSSTLPAGSHLA F*RRARIMSGRQTAIFSGASTLTNRQRGAWILP SCQLPPPGLWFLSFSCQPRLISGQSYRHGRSHRG GSPASPRPGG*GLHCR*SCGPRQGPGTRCCPRR PESRPPGPCCL**R*CRWGGGCPCSAAGTRPGA DR*GGLGRAEARLPGHHASGCRHWMGCTSQPR*A PHLCPAFCVHTWPHWLSVPRDLLNPNS*NRKLW LRGHLPKDTLSQNLT*RDGLQVLPDTYTP*CPT PARLGSEE*TWAKSLALQELPERLPSLH*KSPQR LLRPQNTRPDLECPQQACAARLRRWPTAASGLP PRQQPRAQRSRCAVRLRGCRARHEA*TIHLIKN KTLIKL

Frame 1

FRRGRGLD*GADPAGRRGWTGGAGGVRGRRSVP VGVWASRPRQKARRPCCAGPAAMPFLHGFRRII FEYQPLVDAILGSLGIQDPERQESLDRPSYVASE ESRILVLTELLERKAHSPFYQEGVSNALLKMAE LGLTRAADVLLRHGANLNFEDPVTYYTALHIAV LRNQPDMVELLVHHGADVNRRDRIHESSPLDLAS EEPERLPCLQRLLDLGADVNAADKHGKTALLHA LASSDGVQIHNTENIRLLLEGGADVKATTKDGD TVFTCIIFLLGETVGGDKEEAQMINRFCFQVTRL LLAHGADPSECPAHESLTHICLKSFKLHFPLLR FLLESGAAYNCSLHGASCWSGFHIIFERLCSHP GCTEDESHADLLRKAETVLDLMVTNSQKLQLPEN FDIHPVGSLAEKIQALHFSLRQLESYPPPLKHL CRVAIRLYLQPWPVDVKVKALPLPDRLKWYLLS EHSGSVEDDI**VSGYRNGGHGQLRSACW*MLGQ H*KRVFLIWEMRRDPS*EKPAR*SLKL*SLQW* RVQLWFFQRDCPLPSSGTPSPPHVGKRNRAWID VYTL*IGGQCQGICRSWEGPSLSTPGLRGSCWPH VAVQLERKPGARPVRCHQGPTPAPFPSRFCYSQ KCFWWALREKTLRAPQACFMVLQAKLAPACSCS SGARSAVAPPTRTVLCWQLCFCQTQGLSDHDFC* KAIMSAWLGTDLSEVSPVVRGSLWSH*RADCAT HLRASVPSKLHWSLGIV*TPLGIS*GD*MIYLY YKIKLTFLALTRSLYKISTPPLHSSG*RLPQQPV RKWVAGVRGCCVWGVVSKSLQRSTTLDWQGPPQ CGGPILLFFFFETLCHPGWSAMVQSRLTATSAS QVQAVLLPQPPE*LAL*VPTTMPG*FLYF**RRG FAMLVRLVSNS*PQVIRLPRPFKVLGLQT*ATV PGPHFILVLF*DRVSLCHLGWSAVAQSHLTASS ASRAHPILLPQPPE*LGLQAPAITPG*FFFFLYF K*RRGFTC*PGWSQSPDLVICPPRPPKVLGLQA *ATAPSPILFFTLNACQGAASSPEKTEATGNFP CCRLL*HFKGPRSPLPSRTWHMSTHPTAPSGSTH GLHQPSSVPPLGKDHTEVFKSPATKLGVVSFLF RQPGPWLEGRAGGPGNRLLMWPPYSHMGSTRSP GAPSTQSHLAGEQGLGSCLTAGSAAPSPPGPTLP FEEEPA**VDGRQLYLVVPRHSRTASVAPGSCP AASSPHQDCGSSVSPASPGSSQGKAIDMVDLIG EVLLPLLGQEAEACTADDPADHVKVPAHAAVHVV QNHALLGHVVFDDDDAVGAEAALAAPQELGQVL IGEVAWGGQRQGYQGTMQADVGTGWDVLLSPAE PLICAQHFVCTHGLIGSQSREIY*TPIHRIGNCG SEVIYLKTH*ARI*LDGMAYKSCLIPTHHDAPP LPGLDRRSEPGLRVWHCRSSQRDCLPYTRSPHK GF*DHKIQGQT*SVHSRPVLPASGGGRLLLAACR HVSSPGHSARGVLLG*EGAGLDTKLKLFILLKI KPL*NS

Frame 2

SGGGAAWTEERTRPGAGAGLAEQVGSAAAGAFR SASGHLGLGRKRDGPAARGPRRCRSCTASGGSS SSTSRWWMRFWAPWGSRTPSGRSLWTGPVMSPA RRAESLFSLSCWRGKPTLPFTRKA*ATPCSRWLS WG*RGRPTFSCGMGPISTLKTQSPTTRPCTSPS CGTSRTWWSCWCITGPTLIGGTGSTRVAPWTWP ARSLSACPACSASWTLELMSMPLTSMEKLLCSML WPAATGCRSTILRTFVSYWKEGQTSRPPPKMGT QCSPASSSCLVRPWEGTKRRPR*STASASKSHG CCWHTGPTPASAQPTSPSPTSA*RALNCTSLSCA SSWSPEPPTTAPCTVRPAGLAFTSSLRGSVPTQ AARKTRAMRTSCAKLRLSWISW*PTLRNSSCPK TSISTLWAAWRKRSRPSTSP*GSWRAIPRPSSTC AVWPSGSTFSRGLWM*RSKPCLCPTG*SGTSLA STVAPWKMTSDRSQATGTGDTGSSGQPVGRCWD SIRRGSF*SGR*DGILHEKSQPGRA*SCDHSSGE GCSSGSSRETAPFPPAALQVPPMLERGTGLGLM FIPSELEGSARAFAEAGKDQASAPQGCGALVGR TWQCSWRGSLGHVLCGATKAPPLPLFLPGSVTHR SVSGGLCGRRH*GPHRLVSWCCKPSWPLPVPVA LGPGQPWPHLPELCSAGSCAFAKPRASATMTSA KKQS*VRGWGQTSLKCLLL*GAHFGATEEPTVPP TCEPQSLPSCTGHWELCKHLWG*AEVTK*YIYI IRLN*LSWP*QGPYIKFPPRPSIVQDRGSHSSR *GSGWQV*GDAVFGGLFPSPCKEAPL*TGKGLHS VEVPFYYFFFSRHSVTQAGVQWCNLGSLRPLPP RFKQFSCLSLLSSWHYRCPPPCLADFCIFSRDG VSPCWSGWSQTPDLR*FACLGLSKCWDYRHEPLC PAPILF*FYFETESRSVT*AGVQWRNLTSLQAL PPGLTPFSCLSLLSSWDYRRPPSRLANFFFFCI LSRDGVSPVSQDGLNLLTS*SARLGLPKCWDYRR EPPRPAPFYFLL*MPAKEQPQAQRRLRLRGTSL AAGCCNTLRAPGLHCQAGLGT*APTPQHHQAAP MGSTSPALSLP*VKITLKSSSPQQPSWGWFPSSS GSQAHGWKAGLEAQGIGY*CGHPIPTWGAPGAL EPLPPNPISQESRVWAPASPLVQQHPPRRVPPC LLKKSPHNEWTADSYI*WCLDTHEPPAWRLDLAQ LPAPPTRTVVPQFLLPAPAHLRAKL*TW*ISSG RFSCLSSARRLRPALQMILRTTSRSRHTLLSTS SRITPSWAMLSLMTMMPLGRRLPLQRRRNSARC* SVRWPGEGRGKVTRAPCKRMSALDGMYFSAPLS PSFVPSILCAHMASLALSPERFTKPQFIE*ETV AQRSST*RHIKPEFDLTGWLTSPA*YLHTMMPHP CQAWIGGVNLG*EFGIAGAPRETAFPTLEVPTK ASETTKYKARPRVSTAGLCCPPQEVADCC*RPA ATSAAQGTALEVCC*AKRVQG*TRSLNYSSY*K* NPYKT