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Sequence NM_145863
GTCTCTCGTTTTCGGACGGCTGCAGCATCGCGGTGGGGATCGAAAGCGGGGGCTTCTGGGACGCAGCTCTGGAGACGCGGCCTCGGACCAGCCATTTCGGTGTAGAAGTGGCAGCACGGCAGATTCATCTGAAAACTACATTAAGATGAAGACCTTTGAAGGTTTCTGTGCTTTGCATCTCGCTGCAAGTCAAGGACATT
GGAAAATCGTACAGATTCTTTTAGAAGCTGGGGCAGATCCTAATGCAACTACTTTAGAAGAAACGACACCATTGTTTTTAGCTGTTGAAAATGGACAGAT
AGATGTGTTAAGGCTGTTGCTTCAACACGGAGCAAATGTTAATGGATCCCATTCTATGTGTGGATGGAACTCCTTGCACCAGGCTTCTTTTCAGGAAAAT
GCTGAGATCATAAAATTGCTTCTTAGAAAAGGAGCAAACAAGGAATGCCAGGATGACTTTGGAATCACACCTTTATTTGTGGCTGCTCAGTATGGCAAGC
TAGAAAGCTTGAGCATACTTATTTCATCGGGTGCAAATGTCAATTGTCAAGCCTTGGACAAAGCTACACCCTTGTTCATTGCTGCTCAAGAGGGACACAC
AAAATGTGTGGAGCTTTTGCTCTCCAGTGGGGCAGATCCTGATCTTTACTGTAATGAGGACAGTTGGCAGTTACCTATTCATGCAGCTGCACAAATGGGC
CATACAAAAATCTTGGACTTGTTAATACCACTTACTAACCGGGCCTGTGACACTGGGCTAAACAAAGTAAGCCCTGTTTACTCAGCAGTGTTTGGGGGAC
ATGAAGATTGCCTAGAAATATTACTCCGGAATGGCTACAGCCCAGACGCCCAGGCGTGCCTTGTTTTTGGATTCAGTTCTCCTGTGTGCATGGCTTTCCA
AAAGGACTGTGAGTTCTTTGGAATTGTGAACATTCTTTTGAAATATGGAGCCCAGATAAATGAACTTCATTTGGCATACTGCCTGAAGTACGAGAAGTTT
TCGATATTTCGCTACTTTTTGAGGAAAGGTTGCTCATTGGGACCATGGAACCATATATATGAATTTGTAAATCATGCAATTAAAGCACAAGCAAAATATA
AGGAGTGGTTGCCACATCTTCTGGTTGCTGGATTTGACCCACTGATTCTACTGTGCAATTCTTGGATTGACTCAGTCAGCATTGACACCCTTATCTTCAC
TTTGGAGTTTACTAATTGGAAGACACTTGCACCAGCTGTTGAAAGGATGCTCTCTGCTCGTGCCTCAAACGCTTGGATTCTACAGCAACATATTGCCACT
GTTCCATCCCTGACCCATCTTTGTCGTTTGGAAATTCGGTCCAGTCTAAAATCAGAACGTCTACGGTCTGACAGTTATATTAGTCAGCTGCCACTTCCCA
GAAGCCTACATAATTATTTGCTCTATGAAGACGTTCTGAGGATGTATGAAGTTCCAGAACTGGCAGCTATTCAAGATGGATAAATCAGTGAAACTACTTA
ACACAGCTAATTTTTTTCTCTGAAAAATCATCGAGACAAAAGAGCCACAGAGTACAAGTTTTTATGATTTTATAGTCAAAAGATGATTATTGATTGTGAG
ATAGGTTAGGTTTTGGGGGGCCAGTAGTTCAGTGAGAATGTTTATGTTTACAACTAGCCTTCCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAATTGTAAA
CATCACTTATATTACTTTATTGCAGCTTCATCACCAGTACATTATATGTTGTAATATTTATTTACCTGATCATTTTGATCATTTTCTGCTTTATTTTGCT
AATAAACTGTGATGTTACTTCTAGTGCTAAACATGGCATATTTCCACCTATGATTCGTGTTTACCTGGTATTAGGAGCTCAGAATGGAATGCATAAAGCT
TCACTGGAAGTGTATACAACTGTGGTGTAGAATCTGTTATTATTATCATTATTATTTTATTTAGACTTGACTATCTCTTATGTTTATTAAAGAACATGTT
TTCCT
- refseq_ASB3.F1 (forward primer)
- refseq_ASB3.R1 (reverse primer)
- Single exon skipping
Frame 0
VSRFRTAAASRWGSKAGASGTQLWRRGLGPAISV*KWQHGRFI*KLH*DEDL*RFLCFASRCKSRTL
ENRTDSFRSWGRS*CNYFRRNDTIVFSC*KWTD
RCVKAVASTRSKC*WIPFYVWMELLAPGFFSGK
C*DHKIAS*KRSKQGMPG*LWNHTFICGCSVWQA
RKLEHTYFIGCKCQLSSLGQSYTLVHCCSRGTH
KMCGAFALQWGRS*SLL**GQLAVTYSCSCTNG
PYKNLGLVNTTY*PGL*HWAKQSKPCLLSSVWGT
*RLPRNITPEWLQPRRPGVPCFWIQFSCVHGFP
KGL*VLWNCEHSFEIWSPDK*TSFGILPEVREV
FDISLLFEERLLIGTMEPYI*ICKSCN*STSKI*
GVVATSSGCWI*PTDSTVQFLD*LSQH*HPYLH
FGVY*LEDTCTSC*KDALCSCLKRLDSTATYCH
CSIPDPSLSFGNSVQSKIRTSTV*QLY*SAATSQ
KPT*LFAL*RRSEDV*SSRTGSYSRWINQ*NYL
TQLIFFSEKSSRQKSHRVQVFMIL*SKDDY*L*
DRLGFGGPVVQ*ECLCLQLAFPVKKKKKKKKIVN
ITYITLLQLHHQYIICCNIYLPDHFDHFLLYFA
NKL*CYF*C*TWHISTYDSCLPGIRSSEWNA*S
FTGSVYNCGVESVIIIIIILFRLDYLLCLLKNMF
S
Frame 1
SLVFGRLQHRGGDRKRGLLGRSSGDAASDQPFRCRSGSTADSSENYIKMKTFEGFCALHLAASQGH
WKIVQILLEAGADPNATTLEETTPLFLAVENGQI
DVLRLLLQHGANVNGSHSMCGWNSLHQASFQEN
AEIIKLLLRKGANKECQDDFGITPLFVAAQYGK
LESLSILISSGANVNCQALDKATPLFIAAQEGHT
KCVELLLSSGADPDLYCNEDSWQLPIHAAAQMG
HTKILDLLIPLTNRACDTGLNKVSPVYSAVFGG
HEDCLEILLRNGYSPDAQACLVFGFSSPVCMAFQ
KDCEFFGIVNILLKYGAQINELHLAYCLKYEKF
SIFRYFLRKGCSLGPWNHIYEFVNHAIKAQAKY
KEWLPHLLVAGFDPLILLCNSWIDSVSIDTLIFT
LEFTNWKTLAPAVERMLSARASNAWILQQHIAT
VPSLTHLCRLEIRSSLKSERLRSDSYISQLPLP
RSLHNYLLYEDVLRMYEVPELAAIQDG*ISETT*
HS*FFSLKNHRDKRATEYKFL*FYSQKMIIDCE
IG*VLGGQ*FSENVYVYN*PSQ*KKKKKKKKL*
TSLILLYCSFITSTLYVVIFIYLIILIIFCFILL
INCDVTSSAKHGIFPPMIRVYLVLGAQNGMHKA
SLEVYTTVV*NLLLLSLLFYLDLTISYVY*RTC
FP
Frame 2
LSFSDGCSIAVGIESGGFWDAALETRPRTSHFGVEVAARQIHLKTTLR*RPLKVSVLCISLQVKDI
GKSYRFF*KLGQILMQLL*KKRHHCF*LLKMDR
*MC*GCCFNTEQMLMDPILCVDGTPCTRLLFRKM
LRS*NCFLEKEQTRNARMTLESHLYLWLLSMAS
*KA*AYLFHRVQMSIVKPWTKLHPCSLLLKRDT
QNVWSFCSPVGQILIFTVMRTVGSYLFMQLHKWA
IQKSWTC*YHLLTGPVTLG*TK*ALFTQQCLGD
MKIA*KYYSGMATAQTPRRALFLDSVLLCAWLS
KRTVSSLEL*TFF*NMEPR*MNFIWHTA*STRSF
RYFATF*GKVAHWDHGTIYMNL*IMQLKHKQNI
RSGCHIFWLLDLTH*FYCAILGLTQSALTPLSS
LWSLLIGRHLHQLLKGCSLLVPQTLGFYSNILPL
FHP*PIFVVWKFGPV*NQNVYGLTVILVSCHFP
EAYIIICSMKTF*GCMKFQNWQLFKMDKSVKLL
NTANFFL*KIIETKEPQSTSFYDFIVKR*LLIVR
*VRFWGASSSVRMFMFTTSLPSKKKKKKKKNCK
HHLYYFIAASSPVHYML*YLFT*SF*SFSALFC
**TVMLLLVLNMAYFHL*FVFTWY*ELRMECIKL
HWKCIQLWCRICYYYHYYFI*T*LSLMFIKEHV
F
Sequence NM_016115
GTCTCTCGTTTTCGGACGGCTGCAGCATCGCGGTGGGGATCGAAAGCGGGGGCTTCTGGGACGCAGCTCTGGAGACGCGGCCTCGGACCAGCCATTTCGGTGTAGAAGTGGCAGCACGGCAGACTGGTCAAACAAATGGATTTTACAGAGGCTTACGCGGACACGTGCTCTACAGTTGGACTTGCTGCCAGGGAAGGCAA
TGTTAAAGTCTTAAGGAAACTGCTCAAAAAGGGCCGAAGTGTCGATGTTGCTGATAACAGGGGATGGATGCCAATTCATGAAGCAGCTTATCACAACTCT
GTAGAATGTTTGCAAATGTTAATTAATGCAGATTCATCTGAAAACTACATTAAGATGAAGACCTTTGAAGGTTTCTGTGCTTTGCATCTCGCTGCAAGTC
AAGGACATTGGAAAATCGTACAGATTCTTTTAGAAGCTGGGGCAGATCCTAATGCAACTACTTTAGAAGAAACGACACCATTGTTTTTAGCTGTTGAAAA
TGGACAGATAGATGTGTTAAGGCTGTTGCTTCAACACGGAGCAAATGTTAATGGATCCCATTCTATGTGTGGATGGAACTCCTTGCACCAGGCTTCTTTT
CAGGAAAATGCTGAGATCATAAAATTGCTTCTTAGAAAAGGAGCAAACAAGGAATGCCAGGATGACTTTGGAATCACACCTTTATTTGTGGCTGCTCAGT
ATGGCAAGCTAGAAAGCTTGAGCATACTTATTTCATCGGGTGCAAATGTCAATTGTCAAGCCTTGGACAAAGCTACACCCTTGTTCATTGCTGCTCAAGA
GGGACACACAAAATGTGTGGAGCTTTTGCTCTCCAGTGGGGCAGATCCTGATCTTTACTGTAATGAGGACAGTTGGCAGTTACCTATTCATGCAGCTGCA
CAAATGGGCCATACAAAAATCTTGGACTTGTTAATACCACTTACTAACCGGGCCTGTGACACTGGGCTAAACAAAGTAAGCCCTGTTTACTCAGCAGTGT
TTGGGGGACATGAAGATTGCCTAGAAATATTACTCCGGAATGGCTACAGCCCAGACGCCCAGGCGTGCCTTGTTTTTGGATTCAGTTCTCCTGTGTGCAT
GGCTTTCCAAAAGGACTGTGAGTTCTTTGGAATTGTGAACATTCTTTTGAAATATGGAGCCCAGATAAATGAACTTCATTTGGCATACTGCCTGAAGTAC
GAGAAGTTTTCGATATTTCGCTACTTTTTGAGGAAAGGTTGCTCATTGGGACCATGGAACCATATATATGAATTTGTAAATCATGCAATTAAAGCACAAG
CAAAATATAAGGAGTGGTTGCCACATCTTCTGGTTGCTGGATTTGACCCACTGATTCTACTGTGCAATTCTTGGATTGACTCAGTCAGCATTGACACCCT
TATCTTCACTTTGGAGTTTACTAATTGGAAGACACTTGCACCAGCTGTTGAAAGGATGCTCTCTGCTCGTGCCTCAAACGCTTGGATTCTACAGCAACAT
ATTGCCACTGTTCCATCCCTGACCCATCTTTGTCGTTTGGAAATTCGGTCCAGTCTAAAATCAGAACGTCTACGGTCTGACAGTTATATTAGTCAGCTGC
CACTTCCCAGAAGCCTACATAATTATTTGCTCTATGAAGACGTTCTGAGGATGTATGAAGTTCCAGAACTGGCAGCTATTCAAGATGGATAAATCAGTGA
AACTACTTAACACAGCTAATTTTTTTCTCTGAAAAATCATCGAGACAAAAGAGCCACAGAGTACAAGTTTTTATGATTTTATAGTCAAAAGATGATTATT
GATTGTGAGATAGGTTAGGTTTTGGGGGGCCAGTAGTTCAGTGAGAATGTTTATGTTTACAACTAGCCTTCCCAGTAAAAAAAAAAAAAAAAAAAAAAAA
AATTGTAAACATCACTTATATTACTTTATTGCAGCTTCATCACCAGTACATTATATGTTGTAATATTTATTTACCTGATCATTTTGATCATTTTCTGCTT
TATTTTGCTAATAAACTGTGATGTTACTTCTAGTGCTAAACATGGCATATTTCCACCTATGATTCGTGTTTACCTGGTATTAGGAGCTCAGAATGGAATG
CATAAAGCTTCACTGGAAGTGTATACAACTGTGGTGTAGAATCTGTTATTATTATCATTATTATTTTATTTAGACTTGACTATCTCTTATGTTTATTAAA
GAACATGTTTTCCT
- refseq_ASB3.F1 (forward primer)
- refseq_ASB3.R1 (reverse primer)
- Single exon skipping
Frame 0
VSRFRTAAASRWGSKAGASGTQLWRRGLGPAISV*KWQHGRLVKQMDFTEAYADTCSTVGLAAREGN
VKVLRKLLKKGRSVDVADNRGWMPIHEAAYHNS
VECLQMLINADSSENYIKMKTFEGFCALHLAAS
QGHWKIVQILLEAGADPNATTLEETTPLFLAVEN
GQIDVLRLLLQHGANVNGSHSMCGWNSLHQASF
QENAEIIKLLLRKGANKECQDDFGITPLFVAAQ
YGKLESLSILISSGANVNCQALDKATPLFIAAQE
GHTKCVELLLSSGADPDLYCNEDSWQLPIHAAA
QMGHTKILDLLIPLTNRACDTGLNKVSPVYSAV
FGGHEDCLEILLRNGYSPDAQACLVFGFSSPVCM
AFQKDCEFFGIVNILLKYGAQINELHLAYCLKY
EKFSIFRYFLRKGCSLGPWNHIYEFVNHAIKAQ
AKYKEWLPHLLVAGFDPLILLCNSWIDSVSIDTL
IFTLEFTNWKTLAPAVERMLSARASNAWILQQH
IATVPSLTHLCRLEIRSSLKSERLRSDSYISQL
PLPRSLHNYLLYEDVLRMYEVPELAAIQDG*ISE
TT*HS*FFSLKNHRDKRATEYKFL*FYSQKMII
DCEIG*VLGGQ*FSENVYVYN*PSQ*KKKKKKK
KL*TSLILLYCSFITSTLYVVIFIYLIILIIFCF
ILLINCDVTSSAKHGIFPPMIRVYLVLGAQNGM
HKASLEVYTTVV*NLLLLSLLFYLDLTISYVY*
RTCFP
Frame 1
SLVFGRLQHRGGDRKRGLLGRSSGDAASDQPFRCRSGSTADWSNKWILQRLTRTRALQLDLLPGKA
MLKS*GNCSKRAEVSMLLITGDGCQFMKQLITTL
*NVCKC*LMQIHLKTTLR*RPLKVSVLCISLQV
KDIGKSYRFF*KLGQILMQLL*KKRHHCF*LLK
MDR*MC*GCCFNTEQMLMDPILCVDGTPCTRLLF
RKMLRS*NCFLEKEQTRNARMTLESHLYLWLLS
MAS*KA*AYLFHRVQMSIVKPWTKLHPCSLLLK
RDTQNVWSFCSPVGQILIFTVMRTVGSYLFMQLH
KWAIQKSWTC*YHLLTGPVTLG*TK*ALFTQQC
LGDMKIA*KYYSGMATAQTPRRALFLDSVLLCA
WLSKRTVSSLEL*TFF*NMEPR*MNFIWHTA*ST
RSFRYFATF*GKVAHWDHGTIYMNL*IMQLKHK
QNIRSGCHIFWLLDLTH*FYCAILGLTQSALTP
LSSLWSLLIGRHLHQLLKGCSLLVPQTLGFYSNI
LPLFHP*PIFVVWKFGPV*NQNVYGLTVILVSC
HFPEAYIIICSMKTF*GCMKFQNWQLFKMDKSV
KLLNTANFFL*KIIETKEPQSTSFYDFIVKR*LL
IVR*VRFWGASSSVRMFMFTTSLPSKKKKKKKK
NCKHHLYYFIAASSPVHYML*YLFT*SF*SFSA
LFC**TVMLLLVLNMAYFHL*FVFTWY*ELRMEC
IKLHWKCIQLWCRICYYYHYYFI*T*LSLMFIK
EHVF
Frame 2
LSFSDGCSIAVGIESGGFWDAALETRPRTSHFGVEVAARQTGQTNGFYRGLRGHVLYSWTCCQGRQ
C*SLKETAQKGPKCRCC**QGMDANS*SSLSQL
CRMFANVN*CRFI*KLH*DEDL*RFLCFASRCKS
RTLENRTDSFRSWGRS*CNYFRRNDTIVFSC*K
WTDRCVKAVASTRSKC*WIPFYVWMELLAPGFF
SGKC*DHKIAS*KRSKQGMPG*LWNHTFICGCSV
WQARKLEHTYFIGCKCQLSSLGQSYTLVHCCSR
GTHKMCGAFALQWGRS*SLL**GQLAVTYSCSC
TNGPYKNLGLVNTTY*PGL*HWAKQSKPCLLSSV
WGT*RLPRNITPEWLQPRRPGVPCFWIQFSCVH
GFPKGL*VLWNCEHSFEIWSPDK*TSFGILPEV
REVFDISLLFEERLLIGTMEPYI*ICKSCN*STS
KI*GVVATSSGCWI*PTDSTVQFLD*LSQH*HP
YLHFGVY*LEDTCTSC*KDALCSCLKRLDSTAT
YCHCSIPDPSLSFGNSVQSKIRTSTV*QLY*SAA
TSQKPT*LFAL*RRSEDV*SSRTGSYSRWINQ*
NYLTQLIFFSEKSSRQKSHRVQVFMIL*SKDDY
*L*DRLGFGGPVVQ*ECLCLQLAFPVKKKKKKKK
IVNITYITLLQLHHQYIICCNIYLPDHFDHFLL
YFANKL*CYF*C*TWHISTYDSCLPGIRSSEWN
A*SFTGSVYNCGVESVIIIIIILFRLDYLLCLLK
NMFS
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