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Sequences

Sequence NM_020133

ATGGGTGGAGTCTCGCTTTTTCTTTCCAGTGTTGGCTGACTTACAGCTCTTATAAACTAGTGGCAATTTCTGAACCCAGCCGGCTCCATCTCAGCTTCTG GTTTCTAAGTCCATGTGCCAAAGGCTGCCAGGAAGGAGACGCCTTCCTGAGTCCTGGATCTTTCTTCCTTCTGGAAATCTTTGACTGTGGGTAGTTATTT ATTTCTGAATAAGAGCGTCCACGCATCATGGACCTCGCGGGACTGCTGAAGTCTCAGTTCCTGTGCCACCTGGTCTTCTGCTACGTCTTTATTGCCTCAG GGCTAATCATCAACACCATTCAGCTCTTCACTCTCCTCCTCTGGCCCATTAACAAGCAGCTCTTCCGGAAGATCAACTGCAGACTGTCCTATTGCATCTC AAGCCAGCTGGTGATGCTGCTGGAGTGGTGGTCGGGCACGGAATGCACCATCTTCACGGACCCGCGCGCCTACCTCAAGTATGGGAAGGAAAATGCCATC GTGGTTCTCAACCACAAGTTTGAAATTGACTTTCTGTGTGGCTGGAGCCTGTCCGAACGCTTTGGGCTGTTAGGGGGCTCCAAGGTCCTGGCCAAGAAAG AGCTGGCCTATGTCCCAATTATCGGCTGGATGTGGTACTTCACCGAGATGGTCTTCTGTTCGCGCAAGTGGGAGCAGGATCGCAAGACGGTTGCCACCAG TTTGCAGCACCTCCGGGACTACCCCGAGAAGTATTTTTTCCTGATTCACTGTGAGGGCACACGGTTCACGGAGAAGAAGCATGAGATCAGCATGCAGGTG GCCCGGGCCAAGGGGCTGCCTCGCCTCAAGCATCACCTGTTGCCACGAACCAAGGGCTTCGCCATCACCGTGAGGAGCTTGAGAAATGTAGTTTCAGCTG TATATGACTGTACACTCAATTTCAGAAATAATGAAAATCCAACACTGCTGGGAGTCCTAAACGGAAAGAAATACCATGCAGATTTGTATGTTAGGAGGAT CCCACTGGAAGACATCCCTGAAGACGATGACGAGTGCTCGGCCTGGCTGCACAAGCTCTACCAGGAGAAGGATGCCTTTCAGGAGGAGTACTACAGGACG GGCACCTTCCCAGAGACGCCCATGGTGCCCCCCCGGCGGCCCTGGACCCTCGTGAACTGGCTGTTTTGGGCCTCGCTGGTGCTCTACCCTTTCTTCCAGT TCCTGGTCAGCATGATCAGGAGCGGGTCTTCCCTGACGCTGGCCAGCTTCATCCTCGTCTTCTTTGTGGCCTCCGTGGGAGTTCGATGGATGATTGGTGT GACGGAAATTGACAAGGGCTCTGCCTACGGCAACTCTGACAGCAAGCAGAAACTGAATGACTGACTCAGGGAGGTGTCACCATCCGAAGGGAACCTTGGG GAACTGGTGGCCTCTGCATATCCTCCTTAGTGGGACACGGTGACAAAGGCTGGGTGAGCCCCTGCTGGGCACGGCGGAAGTCACGACCTCTCCAGCCAGG GAGTCTGGTCTCAAGGCCGGATGGGGAGGAAGATGTTTTGTAATCTTTTTTTCCCCATGTGCTTTAGTGGGCTTTGGTTTTCTTTTTGTGCGAGTGTGTG TGAGAATGGCTGTGTGGTGAGTGTGAACTTTGTTCTGTGATCATAGAAAGGGTATTTTAGGCTGCAGGGGAGGGCAGGGCTGGGGACCGAAGGGGACAAG TTCCCCTTTCATCCTTTGGTGCTGAGTTTTCTGTAACCCTTGGTTGCCAGAGATAAAGTGAAAAGTGCTTTAGGTGAGATGACTAAATTATGCCTCCAAG AAAAAAAAATTAAAGTGCTTTTCTGGGTCATCTGTGGTGTATGTTGACATGGACCGCTGCCCCTCCATCCTGCCCTTGCCCGTGGCTTTGGTGTTTCAGA TCCTACTCACGGGAGGCAGCTGCCGCGGAGGTGTGGGCAGCTGAGAGGGGTGGGCAGGCCAGCACAGCTCCTGGCAGGAGACACAGGTCCAGGAGCCCGT GAGCATTTGTGAGAGCAGAGATGGCAAGCACGTGCGTGGAGACCAACGAAGCGTGTCCCTGGCACACAGCAAGGGAGAGTTTCGCTCGGTCTCTGCAGTG CAGTCTCCTGGCGTAAGTCTTGAAGATGGACCCAACTCCCTGAAGAAATGGATCTTGAAATTGAACACAAACATCATGAACGTACAGCCTGGGCATTCTG CAGTGATTTGTGAGATGAGCTTGCATCGACTGTTCCTCTCAGCAGTGACAGCCAAAGTCACCCCTGATAAAATGCAGTTTCACTTTATAAAATGCCTCGC CCCTTCCCAGCATGTAGAGCTTGTCTGCAGCCCTAAAGGAGCCAGGTCCTGCTCTGATGAGGGTCAAATCAGAGCCGACATCCCATGTACAAAAAACCCC CAGGCTTCTGCCTTCCTTCCGGGCTCCTGTCTGCTCCCATTTTACTCCACGGAATAGTTCTGCCCTGTAGCATCTCCAGCGGCCTTTGCAGACTGCTGTC AGCCAACAGGTGCCAAAAATGCAAAAGAAGAAGAAACTAAAAGCAGTGCTATTTTTGCAGAACAAATGCTAGCTATTAAATCATCTTAGGCCAAGACACC AATGGCACTTGGACCGATAGGTATTTGGAGCATGAGTTAGGAAGGATATGTCTTTCTCCAGGTGCGCAGCTGAAAGTTGCTGAGAAAAGCTGCCTGTGTT GCTGTGGTAACATGAGAAGGAATGAACAGCTCTATACAAAAACTGGCCACACATTATAAGGCCTATTCTATGCAGTTATGTTTTTGGCTATTTGCTCATT TAGTCATTGTTTATCAACCAAACTAGGTAAGCCAAGGAGTTACAAGACAGCTCTAGAGAAGCTCCCTCTGCTTACAGGACAATAATGCATTGTTTGTTTG TTGATACAAATGAATTATTTGAATCCACAGAAGTGAAAAAGGGAAAAAATCCTGGCTGACTTGTGGGAAGAGCGTACTGCAGAGCCTAGGGCCTGAGTTA ACTCGCTGGCGGCCCTGCAGGCAGGCTTGATGGGAGCGCCCAGCACATCTGCCCACCCTGCACAGGGCATGAAGCACCGTGTGCAGAGATCTGTGGGCAA AAGCCCAGGACGTGACCTTAGGCAAGAGGGGTCCTTGGAGAAGTGGAGGTGTGCATGGTGTGTAGCCTTGCGTGTCTGTGAGCACGCCCTAGTGGACCTG CGCCTGGATTATCGGGCCAGTCTCTCGGAGGGGTCCGTGATGCTGAGTTACGCCATTCACCTGAACTCTGCAGCTCATCGGCTGTAGGATTTGGGTGAGA CACTTTTCCTTGCTGACTCATTTCCCCATCTGCAAAATGGGGGTGACGATATCTACCTCTTGGGGTGTGTCAAGGGTAAAAGAGCACACTTTAAGAATCC TAAAGCACTAAGCCAAGGATGAAAGACTAGAAAGCTGCCTCTATGTTGCTACTCTCAAAAATGCTCAGAAATTTTCTTGTCGTACTAAAAATGTGTTATT TTTCTCATTTTCATTCTTCCATTCAACACAGAAGTAATCTAAGTAAGTTCTAAAAACATAGATCCGGGGGCCCACAGACATTCAATTTTCTTCTGAATTG GCTCTGGTATCACACAACACACAAGCAAGCAAACAGCCTGCCTGCTAGGGCCTTGGGTTCCAGGCTGGAGGAATGCAGGCAGAAATCTCTTAGGAAATAA CTAAGTGAGCGCCAAGTTTTGAATTGCCTTAGAGCCGAAAGAGAATGTGGCGTGCTGGGGAGAGATCTGGACCTCTTTCTCATCAGTCATTTGACCTGTC CACGAAGAACAATTTTGTTATGCAGCCTGGGAACCTGGTTTCCTTACACCCTGCGCCCTTAATGACACAGACAACCACGGGTACTCCTGGACGGAAGCTC ATCACGCCTCCTCAAATACCTTCACCCATGTTCAGGTCACACGGAGGTCACCATCTCCTCAGCACACAACAACTGCTTTGGTTCCCAGGGTTATTTTTTC TACGAGTTGAAAACCCACGGAGCCTAAGCCATCTTTTTGTCCCCTAAGGAGAGCCCCATAGACTAAGAGCTACACTTCCTGTCTGTCTTTCCCACCTTTC CATCTCATTCTAGCTTAAAGCTTATCACGCTACTGAGAGCCTGTTCTGTAGTTCAAGGTGGCAGAGAAAAATACGAACCTTGAGTGAAATCTGGAGAGCA GTGCAGGGTCATCCCCAGACGTGACGGGCCATATCCTAGGCGACTCCTGCAGCTTATAGAAGCAGGGAATTTAATGGACTTCTTCCAGGAAAGCCAGCCC ACGGGGACTCTTAAAAAGACACCAACCATTTGGAAGATGCAGCTTGAGGTCTGGGGGACTGGACTGATTCTGCCACCATATAGTGAACACGATTAATCGG GAGGAAGAAGTAAGGCACTCTGTGAGTTAGTCTACCACCCTATTGACTTTGACAGAGAGAAAACCAACTTTTTTCATATGATCACACAGTAGTACTGGAA TATCTCGACTGCAATATCCTCTAAACAAATGCATGCAAAGCTTTGTAAAAGCAGCTGATGATTAAAAAAATTTTAAAACATTAGGGTTTTTCTAATATAT CCATATGTGGGACAGTGATTTAAATGTCATTAATTGTCCAGCCACAACTTGTAACAGTGATGTGAAAGACACAAAAAAGGCCCCACTGGAGTGGAGGAGT GGTCTGGCTGGTCCCCAAGCAAAACAGAATGGGCTTCGCATAGGCTTTAGAACTTATTCATAAATATTGACCAAAGTGTGAAGCAAGGTATCCAGTGAAG ATTCTCTAGGGTCTCTACTTCTGCTCAAGAGATTTAATCCAGCTTTCTCTAAAGCAGTGGTCCAGACTTGCCACATACTAGGTTAAAGATAAAGTGAGAA AAACAAATACTTTGGTTTGCACGTCTCTTGAGGGGCCCTAGGCTGTATGGAGGAATATTTAGAGGAATATTTGTGAGCTGCCATTTGTGTTTAAAAGAAC ATGAAAGCAAGACGGGCCCTAACCCGCCTGTGTCTGTAGCTGACCAGGAGGCCACTGCTTCCTTCCCCCAGAGCTTCTAGGAGAGCCCCCCAAAATGCAA CTCTCCAAGGTCCTGTGGAGTAGTCCCCTCATAATGCCTCCACCACACCTGTGTCTCACGATGCCTTAACTGACGTAGCTCCTAAGTGATGTTCACAGAG AAGGAAATACTTAGGCAAGCCCATGTGTAACATGCTCACAGATGTGCTGGCTTTGCCAATGCTTTCGGACTCCACCATGCTTTCTGATTCTTCTGGTCCT AGTCACGCCTTGGCACGGGGCAGAAGCGGCCGAGCTATGCTGACAGGTGCTGCTGAGAGCCAACAGCCATTCAGCCGAGAAGGCGTTTCTAGGTGAGGGC ATGAGAGCTGGCATTCCAGGCTGCTGAGTGCGGCATCTGCAGGTGCACCACATTGCTCCACGGTGTGTTCAGAAGAGACCAGCATCTTGAATCTCAGGCT CTGGGCTCATTCTTTGCCTCTGCCAAGGGACCATTCAGGGGCTTCAAGCCAGCTGCTGAAAATCCCCCTTTTCTGGATTTTTCCCCATCTATATAAGGAT ATGGTGGCTGTGTGGACAAGAAATTGGTTTCATCTCAGAAGGGTGCTTCCCTACAAAGATCTTCAGAAATTTAATTGAAAACAATTTTTTGAACTTTTTT TGTTTGGCCAGGATGAATGAAGCTGAAGTCACATCCTGAATGTGCATGCCAGACAAAGGGTAAGCAAGCAGTCTTTACAAATGCCAATCCCTCTGCACTC CAAAACCGGAGCCCGTAATGAAGGCCCAGGTTCCAGTCATCACAGGATCCCCCAAGCCAGCACAGTGCCTGATGCCTCCCTAGGCATTAGCACATCCCAG CACTCTAATTCACATCCTCAGCATCCCTGCCTCCTGCATCCAGGGCTTAGGTTGAGTGTTTCCAGAGTGTCAAGTACATTTAAACACAAACCAAATACTC CAGGGTTTTGACCTGTCACAATGGCTTTTGAAAACAACAGACGTACAATTTGAGAGAAGGACTCGATGGACTTCTTAGGCTGAGGACTAAGGACATGTTG CTGTTCCTCTTGGTCCAGGACATTCAATACCATTCACAGCATTCATCACCAACTGACATAGTATCGATCACGACATTCAACTGTTTGAAATTACTGCTAT TTTGGTCAAAAACTAAGAAATCAGAAATTGCACCTACATAGTGTGTCTCCTCATTGGAGTCCCATCTCTCTGAGCACAGGGATGGGCTCCTGAGCTCACA GCCCTGCCCTGCCCCCCCAGCAGGGCCTCACCCTCATGGCCCTCAGTGAATAGTGACTACATGAAGGGGGCACTTACTAGAGGCCTGAGTAATGGAATTT CAATTAGCTTCTGCTTCCCTTTTCTAAAAAAGAACCACCTTTCTTAAGAATAATTAGGCCAAAATAAACTTGAGTAATTTGTACAGTCAATATTTTGAAG TTTCGGAGTTACCAGTGAAATAGCAAAGCTGTTCTCCAGGAAAGGACATCCAGGTTCCCACCCAGGCCTTCCTTCCTCAGCTCCTGCAGCCTCATCCTAG TTTAATATGAAGGTTGTGAATGGGTTAAAAAGCAGTTCTTAAGCACCTACTTAGATCTTTTCAGTATCTGGAAATAGCACCCAGGGCTGTGTTAGTAAAG CTTTTCACTGGGTAAGAGTCATACAATTTTTACATTTAATTTGTTTTTTTAAAACAAGGCAGTCTTGTTTTACATATTGGAATGACTAGATATTTTTTGA CATTTCTGGCACCTCTGGTTCTGCTGAATTTTAAGGGAGGTGAGTGGTATGTACACAAAGTTAAGATTGCTGCATAATAATTCCTTTGCATTTCCAAAAT AAATTACACAAATGTCACTTTTCCTTTTCTATTCCATAGTACTAGTTTTAGCCAGATCTTTGCCAAACATTCTAGGACATTCAGAAAAGTCATCCATGGA ATTTTGGAATGCCATTCTCTCTGTATCTTTGCAGATCATGAGGAAGGAAAGTTGATGAACTAGTACGGGATGGCTCAGACTTGTAAAGTGTCTAAGTCAC AACGCTGCAGAGCCAGCTTCCGGGTAGCAATGGCATGGCCTCAGCTGTGCCAAGGCTACAACTGGAGCTTCCTTACTCGCATCTGAGGCAAACACAGCAA GGCTGGCCCAGGCCGCCCACAGTCAACAGATGTTCACGGGAGATGGATCAGCCCCTCTTTTGCTTGTTCTATCAAGGAAAGAGATCTAACCATGGGCAAG GGTGGAACACACCCGGTTCAGGTCAGGGAATGAGTTGCTCTAAGTGAACAGTGAATTCTTACTTCTCAGCCTGTATCAATTGTACCCAGGGTTCATGATT ACTGTGACCCACTAAGGGATGATCTTTGAGGAAACAGACCTGGAGGGAGGTAATTTAAGGAGGGAAAGCGAGTGGGTTTTACAGACTGCTTTGTGGCGAC ATTCAGGAACAGACCATTCAATGGATGACTTTTCAGTACATAAAAGCAAGCAGTAATGGCCAGGAGAGAGCAAAGATGCCCTAAGAATGACATCAGACTG ACCAAATGTGCCTTTTCAAATAAGTTATGAGAACGAATGAATTGGAATTAGAGCAAGGTATTGGACAGAACCATGCTAGTCTTTGAGAGAACGTGTGGAT TGGGTGGATGTTTGGACCGAATGAATGGTAACTTTTTAATGTGAAACAGAGAACTGATATATGGGCTGTCATGTCACCCTAGGGTACTGTCGCTCTGTCA TGTGTAGCAATCTTTCCAAATTTTTGTTGGTGGGCTGAACAAAGATAAAACTAATAAAGTATGTGTGGATACCACTAGA

refseq_AGPAT4.F1 (forward primer)
refseq_AGPAT4.R1 (reverse primer)
Single exon skipping

Frame 0

MGGVSLFLSSVG*LTALIN*WQFLNPAGSISAS GF*VHVPKAARKETPS*VLDLSSFWKSLTVGSYL FLNKSVHASWTSRDC*SLSSCATWSSATSLLPQ G*SSTPFSSSLSSSGPLTSSSSGRSTADCPIAS QASW*CCWSGGRARNAPSSRTRAPTSSMGRKMPS WFSTTSLKLTFCVAGACPNALGC*GAPRSWPRK SWPMSQLSAGCGTSPRWSSVRASGSRIARRLPP VCSTSGTTPRSIFS*FTVRAHGSRRRSMRSACRW PGPRGCLASSITCCHEPRASPSP*GA*EM*FQL YMTVHSISEIMKIQHCWES*TERNTMQICMLGG SHWKTSLKTMTSARPGCTSSTRRRMPFRRSTTGR APSQRRPWCPPGGPGPS*TGCFGPRWCSTLSSS SWSA*SGAGLP*RWPASSSSSLWPPWEFDG*LV *RKLTRALPTATLTASRN*MTDSGRCHHPKGTLG NWWPLHILLSGTR*QRLGEPLLGTAEVTTSPAR ESGLKAGWGGRCFVIFFSPCALVGFGFLFVRVC VRMAVW*V*TLFCDHRKGILGCRGGQGWGPKGTS SPFILWC*VFCNPWLPEIK*KVL*VR*LNYASK KKKLKCFSGSSVVYVDMDRCPSILPLPVALVFQ ILLTGGSCRGGVGS*EGWAGQHSSWQETQVQEPV SICESRDGKHVRGDQRSVSLAHSKGEFRSVSAV QSPGVSLEDGPNSLKKWILKLNTNIMNVQPGHS AVICEMSLHRLFLSAVTAKVTPDKMQFHFIKCLA PSQHVELVCSPKGARSCSDEGQIRADIPCTKNP QASAFLPGSCLLPFYSTE*FCPVASPAAFADCC QPTGAKNAKEEETKSSAIFAEQMLAIKSS*AKTP MALGPIGIWSMS*EGYVFLQVRS*KLLRKAACV AVVT*EGMNSSIQKLATHYKAYSMQLCFWLFAH LVIVYQPN*VSQGVTRQL*RSSLCLQDNNALFVC *YK*II*IHRSEKGKKSWLTCGKSVLQSLGPEL TRWRPCRQA*WERPAHLPTLHRA*STVCRDLWA KAQDVTLGKRGPWRSGGVHGV*PCVSVSTP*WTC AWIIGPVSRRGP*C*VTPFT*TLQLIGCRIWVR HFSLLTHFPICKMGVTISTSWGVSRVKEHTLRI LKH*AKDERLESCLYVATLKNAQKFSCRTKNVLF FSFSFFHSTQK*SK*VLKT*IRGPTDIQFSSEL ALVSHNTQASKQPAC*GLGFQAGGMQAEIS*EI TK*APSFELP*SRKRMWRAGERSGPLSHQSFDLS TKNNFVMQPGNLVSLHPAPLMTQTTTGTPGRKL ITPPQIPSPMFRSHGGHHLLSTQQLLWFPGLFF LRVENPRSLSHLFVP*GEPHRLRATLPVCLSHLS ISF*LKAYHATESLFCSSRWQRKIRTLSEIWRA VQGHPQT*RAIS*ATPAAYRSREFNGLLPGKPA HGDS*KDTNHLEDAA*GLGDWTDSATI**TRLIG RKK*GTL*VSLPPY*L*QRENQLFSYDHTVVLE YLDCNIL*TNACKAL*KQLMIKKILKH*GFSNI SICGTVI*MSLIVQPQLVTVM*KTQKRPHWSGGV VWLVPKQNRMGFA*ALELIHKY*PKCEARYPVK IL*GLYFCSRDLIQLSLKQWSRLATY*VKDKVR KTNTLVCTSLEGP*AVWRNI*RNICELPFVFKRT *KQDGP*PACVCS*PGGHCFLPPELLGEPPKMQ LSKVLWSSPLIMPPPHLCLTMP*LT*LLSDVHR EGNT*ASPCVTCSQMCWLCQCFRTPPCFLILLVL VTPWHGAEAAELC*QVLLRANSHSAEKAFLGEG MRAGIPGC*VRHLQVHHIAPRCVQKRPAS*ISG SGLILCLCQGTIQGLQASC*KSPFSGFFPIYIRI WWLCGQEIGFISEGCFPTKIFRNLIENNFLNFF CLARMNEAEVTS*MCMPDKG*ASSLYKCQSLCT PKPEPVMKAQVPVITGSPKPAQCLMPP*ALAHPS TLIHILSIPASCIQGLG*VFPECQVHLNTNQIL QGFDLSQWLLKTTDVQFERRTRWTS*AED*GHV AVPLGPGHSIPFTAFITN*HSIDHDIQLFEITAI LVKN*EIRNCTYIVCLLIGVPSL*AQGWAPELT ALPCPPSRASPSWPSVNSDYMKGALTRGLSNGI SISFCFPFLKKNHLS*E*LGQNKLE*FVQSIF*S FGVTSEIAKLFSRKGHPGSHPGLPSSAPAASS* FNMKVVNGLKSSS*APT*IFSVSGNSTQGCVSK AFHWVRVIQFLHLICFFKTRQSCFTYWND*IFFD ISGTSGSAEF*GR*VVCTQS*DCCIIIPLHFQN KLHKCHFSFSIP*Y*F*PDLCQTF*DIQKSHPW NFGMPFSLYLCRS*GRKVDELVRDGSDL*SV*VT TLQSQLPGSNGMASAVPRLQLELPYSHLRQTQQ GWPRPPTVNRCSREMDQPLFCLFYQGKRSNHGQ GWNTPGSGQGMSCSK*TVNSYFSACINCTQGS*L L*PTKG*SLRKQTWREVI*GGKASGFYRLLCGD IQEQTIQWMTFQYIKASSNGQERAKMP*E*HQT DQMCLFK*VMRTNELELEQGIGQNHASL*ENVWI GWMFGPNEW*LFNVKQRTDIWAVMSP*GTVALS CVAIFPNFCWWAEQR*N**SMCGYH*

Frame 1

WVESRFFFPVLADLQLL*TSGNF*TQPAPSQLL VSKSMCQRLPGRRRLPESWIFLPSGNL*LWVVI YF*IRASTHHGPRGTAEVSVPVPPGLLLRLYCLR ANHQHHSALHSPPLAH*QAALPEDQLQTVLLHL KPAGDAAGVVVGHGMHHLHGPARLPQVWEGKCH RGSQPQV*N*LSVWLEPVRTLWAVRGLQGPGQER AGLCPNYRLDVVLHRDGLLFAQVGAGSQDGCHQ FAAPPGLPREVFFPDSL*GHTVHGEEA*DQHAG GPGQGAASPQASPVATNQGLRHHREELEKCSFSC I*LYTQFQK**KSNTAGSPKRKEIPCRFVC*ED PTGRHP*RR*RVLGLAAQALPGEGCLSGGVLQD GHLPRDAHGAPPAALDPRELAVLGLAGALPFLPV PGQHDQERVFPDAGQLHPRLLCGLRGSSMDDWC DGN*QGLCLRQL*QQAETE*LTQGGVTIRREPW GTGGLCISSLVGHGDKGWVSPCWARRKSRPLQPG SLVSRPDGEEDVL*SFFPHVL*WALVFFLCECV *EWLCGECELCSVIIERVF*AAGEGRAGDRRGQ VPLSSFGAEFSVTLGCQR*SEKCFR*DD*IMPPR KKN*SAFLGHLWCMLTWTAAPPSCPCPWLWCFR SYSREAAAAEVWAAERGGQASTAPGRRHRSRSP *AFVRAEMASTCVETNEACPWHTARESFARSLQC SLLA*VLKMDPTP*RNGS*N*TQTS*TYSLGIL Q*FVR*ACIDCSSQQ*QPKSPLIKCSFTL*NAS PLPSM*SLSAALKEPGPALMRVKSEPTSHVQKTP RLLPSFRAPVCSHFTPRNSSAL*HLQRPLQTAV SQQVPKMQKKKKLKAVLFLQNKC*LLNHLRPRH QWHLDR*VFGA*VRKDMSFSRCAAESC*EKLPVL LW*HEKE*TALYKNWPHIIRPILCSYVFGYLLI *SLFINQTR*AKELQDSSREAPSAYRTIMHCLF VDTNELFESTEVKKGKNPG*LVGRAYCRA*GLS* LAGGPAGRLDGSAQHICPPCTGHEAPCAEICGQ KPRT*P*ARGVLGEVEVCMVCSLACL*ARPSGP APGLSGQSLGGVRDAELRHSPELCSSSAVGFG*D TFPC*LISPSAKWG*RYLPLGVCQG*KSTL*ES *STKPRMKD*KAASMLLLSKMLRNFLVVLKMCY FSHFHSSIQHRSNLSKF*KHRSGGPQTFNFLLNW LWYHTTHKQANSLPARALGSRLEECRQKSLRK* LSERQVLNCLRAERECGVLGRDLDLFLISHLTC PRRTILLCSLGTWFPYTLRP**HRQPRVLLDGSS SRLLKYLHPCSGHTEVTISSAHNNCFGSQGYFF YELKTHGA*AIFLSPKESPID*ELHFLSVFPTF PSHSSLKLITLLRACSVVQGGREKYEP*VKSGEQ CRVIPRRDGPYPRRLLQLIEAGNLMDFFQESQP TGTLKKTPTIWKMQLEVWGTGLILPPYSEHD*S GGRSKALCELVYHPIDFDREKTNFFHMITQ*YWN ISTAISSKQMHAKLCKSS**LKKF*NIRVFLIY PYVGQ*FKCH*LSSHNL*Q*CERHKKGPTGVEE WSGWSPSKTEWASHRL*NLFINIDQSVKQGIQ*R FSRVSTSAQEI*SSFL*SSGPDLPHTRLKIK*E KQILWFARLLRGPRLYGGIFRGIFVSCHLCLKE HESKTGPNPPVSVADQEATASFPQSF*ESPPKCN SPRSCGVVPS*CLHHTCVSRCLN*RSS*VMFTE KEILRQAHV*HAHRCAGFANAFGLHHAF*FFWS *SRLGTGQKRPSYADRCC*EPTAIQPRRRF*VRA *ELAFQAAECGICRCTTLLHGVFRRDQHLESQA LGSFFASAKGPFRGFKPAAENPPFLDFSPSI*G YGGCVDKKLVSSQKGASLQRSSEI*LKTIF*TFF VWPG*MKLKSHPECACQTKGKQAVFTNANPSAL QNRSP**RPRFQSSQDPPSQHSA*CLPRH*HIP AL*FTSSASLPPASRA*VECFQSVKYI*TQTKYS RVLTCHNGF*KQQTYNLREGLDGLLRLRTKDML LFLLVQDIQYHSQHSSPTDIVSITTFNCLKLLL FWSKTKKSEIAPT*CVSSLESHLSEHRDGLLSSQ PCPAPPAGPHPHGPQ*IVTT*RGHLLEA*VMEF QLASASLF*KRTTFLKNN*AKINLSNLYSQYFE VSELPVK*QSCSPGKDIQVPTQAFLPQLLQPHPS LI*RL*MG*KAVLKHLLRSFQYLEIAPRAVLVK LFTG*ESYNFYI*FVFLKQGSLVLHIGMTRYFL TFLAPLVLLNFKGGEWYVHKVKIAA**FLCISKI NYTNVTFPFLFHSTSFSQIFAKHSRTFRKVIHG ILECHSLCIFADHEEGKLMN*YGMAQTCKVSKS QRCRASFRVAMAWPQLCQGYNWSFLTRI*GKHSK AGPGRPQSTDVHGRWISPSFACSIKERDLTMGK GGTHPVQVRE*VALSEQ*ILTSQPVSIVPRVHD YCDPLRDDL*GNRPGGR*FKEGKRVGFTDCFVAT FRNRPFNG*LFST*KQAVMARREQRCPKNDIRL TKCAFSNKL*ERMNWN*SKVLDRTMLVFERTCG LGGCLDRMNGNFLM*NRELIYGLSCHPRVLSLCH V*QSFQIFVGGLNKDKTNKVCVDTTR

Frame 2

GWSLAFSFQCWLTYSSYKLVAISEPSRLHLSFW FLSPCAKGCQEGDAFLSPGSFFLLEIFDCG*LF ISE*ERPRIMDLAGLLKSQFLCHLVFCYVFIAS GLIINTIQLFTLLLWPINKQLFRKINCRLSYCIS SQLVMLLEWWSGTECTIFTDPRAYLKYGKENAI VVLNHKFEIDFLCGWSLSERFGLLGGSKVLAKK ELAYVPIIGWMWYFTEMVFCSRKWEQDRKTVATS LQHLRDYPEKYFFLIHCEGTRFTEKKHEISMQV ARAKGLPRLKHHLLPRTKGFAITVRSLRNVVSA VYDCTLNFRNNENPTLLGVLNGKKYHADLYVRRI PLEDIPEDDDECSAWLHKLYQEKDAFQEEYYRT GTFPETPMVPPRRPWTLVNWLFWASLVLYPFFQ FLVSMIRSGSSLTLASFILVFFVASVGVRWMIGV TEIDKGSAYGNSDSKQKLND*LREVSPSEGNLG ELVASAYPP*WDTVTKAG*APAGHGGSHDLSSQ GVWSQGRMGRKMFCNLFFPMCFSGLWFSFCASVC ENGCVVSVNFVL*S*KGYFRLQGRAGLGTEGDK FPFHPLVLSFL*PLVARDKVKSALGEMTKLCLQ EKKIKVLFWVICGVC*HGPLPLHPALARGFGVSD PTHGRQLPRRCGQLRGVGRPAQLLAGDTGPGAR EHL*EQRWQARAWRPTKRVPGTQQGRVSLGLCS AVSWRKS*RWTQLPEEMDLEIEHKHHERTAWAFC SDL*DELASTVPLSSDSQSHP**NAVSLYKMPR PFPACRACLQP*RSQVLL**GSNQSRHPMYKKP PGFCLPSGLLSAPILLHGIVLPCSISSGLCRLLS ANRCQKCKRRRN*KQCYFCRTNASY*IILGQDT NGTWTDRYLEHELGRICLSPGAQLKVAEKSCLC CCGNMRRNEQLYTKTGHTL*GLFYAVMFLAICSF SHCLSTKLGKPRSYKTALEKLPLLTGQ*CIVCL LIQMNYLNPQK*KREKILADLWEERTAEPRA*V NSLAALQAGLMGAPSTSAHPAQGMKHRVQRSVGK SPGRDLRQEGSLEKWRCAWCVALRVCEHALVDL RLDYRASLSEGSVMLSYAIHLNSAAHRL*DLGE TLFLADSFPHLQNGGDDIYLLGCVKGKRAHFKNP KALSQG*KTRKLPLCCYSQKCSEIFLSY*KCVI FLIFILPFNTEVI*VSSKNIDPGAHRHSIFF*I GSGITQHTSKQTACLLGPWVPGWRNAGRNLLGNN *VSAKF*IALEPKENVACWGEIWTSFSSVI*PV HEEQFCYAAWEPGFLTPCALNDTDNHGYSWTEA HHASSNTFTHVQVTRRSPSPQHTTTALVPRVIFS TS*KPTEPKPSFCPLRRAP*TKSYTSCLSFPPF HLILA*SLSRY*EPVL*FKVAEKNTNLE*NLES SAGSSPDVTGHILGDSCSL*KQGI*WTSSRKASP RGLLKRHQPFGRCSLRSGGLD*FCHHIVNTINR EEEVRHSVS*STTLLTLTERKPTFFI*SHSSTG ISRLQYPLNKCMQSFVKAADD*KNFKTLGFF*YI HMWDSDLNVINCPATTCNSDVKDTKKAPLEWRS GLAGPQAKQNGLRIGFRTYS*ILTKV*SKVSSE DSLGSLLLLKRFNPAFSKAVVQTCHILG*R*SEK NKYFGLHVS*GALGCMEEYLEEYL*AAICV*KN MKARRALTRLCL*LTRRPLLPSPRASRRAPQNA TLQGPVE*SPHNASTTPVSHDALTDVAPK*CSQR RKYLGKPMCNMLTDVLALPMLSDSTMLSDSSGP SHALARGRSGRAMLTGAAESQQPFSREGVSR*G HESWHSRLLSAASAGAPHCSTVCSEETSILNLRL WAHSLPLPRDHSGASSQLLKIPLFWIFPHLYKD MVAVWTRNWFHLRRVLPYKDLQKFN*KQFFELF LFGQDE*S*SHILNVHARQRVSKQSLQMPIPLHS KTGARNEGPGSSHHRIPQASTVPDASLGISTSQ HSNSHPQHPCLLHPGLRLSVSRVSSTFKHKPNT PGF*PVTMAFENNRRTI*EKDSMDFLG*GLRTCC CSSWSRTFNTIHSIHHQLT*YRSRHSTV*NYCY FGQKLRNQKLHLHSVSPHWSPISLSTGMGS*AH SPALPPQQGLTLMALSE**LHEGGTY*RPE*WNF N*LLLPFSKKEPPFLRIIRPK*T*VICTVNILK FRSYQ*NSKAVLQERTSRFPPRPSFLSSCSLIL V*YEGCEWVKKQFLSTYLDLFSIWK*HPGLC**S FSLGKSHTIFTFNLFF*NKAVLFYILE*LDIF* HFWHLWFC*ILREVSGMYTKLRLLHNNSFAFPK *ITQMSLFLFYSIVLVLARSLPNILGHSEKSSME FWNAILSVSLQIMRKES**TSTGWLRLVKCLSH NAAEPASG*QWHGLSCAKATTGASLLASEANTA RLAQAAHSQQMFTGDGSAPLLLVLSRKEI*PWAR VEHTRFRSGNELL*VNSEFLLLSLYQLYPGFMI TVTH*GMIFEETDLEGGNLRRESEWVLQTALWR HSGTDHSMDDFSVHKSKQ*WPGESKDALRMTSD* PNVPFQISYENE*IGIRARYWTEPC*SLRERVD WVDVWTE*MVTF*CETEN*YMGCHVTLGYCRSV MCSNLSKFLLVG*TKIKLIKYVWIPL

Sequence NM_001012733

ATGGGTGGAGTCTCGCTTTTTCTTTCCAGTGTTGGCTGACTTACAGCTCTTATAAACTAGTGGCAATTTCTGAACCCAGCCGGCTCCATCTCAGCTTCTG GTTTCTAAGTCCATGTGCCAAAGGCTGCCAGGAAGGAGACGCCTTCCTGAGTCCTGGATCTTTCTTCCTTCTGGAAATCTTTGACTGTGGGTAGTTATTT ATTTCTGAATAAGAGCGTCCACGCATCATGGACCTCGCGGGACTGCTGAAGTCTCAGTTCCTGTGCCACCTGGTCTTCTGCTACGTCTTTATTGCCTCAG GGCTAATCATCAACACCATTCAGCTCTTCACTCTCCTCCTCTGGCCCATTAACAAGCAGCTCTTCCGGAAGATCAACTGCAGACTGTCCTATTGCATCTC AAGCCAGCTGGTGATGCTGCTGGAGTGGTGGTCGGGCACGGAATGCACCATCTTCACGGACCCGCGCGCCTACCTCAAGTATGGGAAGGAAAATGCCATC GTGGTTCTCAACCACAAGTTTGAAATTGACTTTCTGTGTGGCTGGAGCCTGTCCGAACGCTTTGGGCTGTTAGGGTTTGACCTCTTGCACGTAGCTTGCA TGCCTTTGGAACTGGGTCTCTTCAGTTGACTTTTGTCGTCTTCAACACACTGCAGCGTGTAGCGGTTGAGCCTGTGTCACATAGCTGAATCAGAGTCATC TGCGGTACTCAGGGCTCCAAGGTCCTGGCCAAGAAAGAGCTGGCCTATGTCCCAATTATCGGCTGGATGTGGTACTTCACCGAGATGGTCTTCTGTTCGC GCAAGTGGGAGCAGGATCGCAAGACGGTTGCCACCAGTTTGCAGCACCTCCGGGACTACCCCGAGAAGTATTTTTTCCTGATTCACTGTGAGGGCACACG GTTCACGGAGAAGAAGCATGAGATCAGCATGCAGGTGGCCCGGGCCAAGGGGCTGCCTCGCCTCAAGCATCACCTGTTGCCACGAACCAAGGGCTTCGCC ATCACCGTGAGGAGCTTGAGAAATGTAGTTTCAGCTGTATATGACTGTACACTCAATTTCAGAAATAATGAAAATCCAACACTGCTGGGAGTCCTAAACG GAAAGAAATACCATGCAGATTTGTATGTTAGGAGGATCCCACTGGAAGACATCCCTGAAGACGATGACGAGTGCTCGGCCTGGCTGCACAAGCTCTACCA GGAGAAGGATGCCTTTCAGGAGGAGTACTACAGGACGGGCACCTTCCCAGAGACGCCCATGGTGCCCCCCCGGCGGCCCTGGACCCTCGTGAACTGGCTG TTTTGGGCCTCGCTGGTGCTCTACCCTTTCTTCCAGTTCCTGGTCAGCATGATCAGGAGCGGGTCTTCCCTGACGCTGGCCAGCTTCATCCTCGTCTTCT TTGTGGCCTCCGTGGGAGTTCGATGGATGATTGGTGTGACGGAAATTGACAAGGGCTCTGCCTACGGCAACTCTGACAGCAAGCAGAAACTGAATGACTG ACTCAGGGAGGTGTCACCATCCGAAGGGAACCTTGGGGAACTGGTGGCCTCTGCATATCCTCCTTAGTGGGACACGGTGACAAAGGCTGGGTGAGCCCCT GCTGGGCACGGCGGAAGTCACGACCTCTCCAGCCAGGGAGTCTGGTCTCAAGGCCGGATGGGGAGGAAGATGTTTTGTAATCTTTTTTTCCCCATGTGCT TTAGTGGGCTTTGGTTTTCTTTTTGTGCGAGTGTGTGTGAGAATGGCTGTGTGGTGAGTGTGAACTTTGTTCTGTGATCATAGAAAGGGTATTTTAGGCT GCAGGGGAGGGCAGGGCTGGGGACCGAAGGGGACAAGTTCCCCTTTCATCCTTTGGTGCTGAGTTTTCTGTAACCCTTGGTTGCCAGAGATAAAGTGAAA AGTGCTTTAGGTGAGATGACTAAATTATGCCTCCAAGAAAAAAAAATTAAAGTGCTTTTCTGGGTCATCTGTGGTGTATGTTGACATGGACCGCTGCCCC TCCATCCTGCCCTTGCCCGTGGCTTTGGTGTTTCAGATCCTACTCACGGGAGGCAGCTGCCGCGGAGGTGTGGGCAGCTGAGAGGGGTGGGCAGGCCAGC ACAGCTCCTGGCAGGAGACACAGGTCCAGGAGCCCGTGAGCATTTGTGAGAGCAGAGATGGCAAGCACGTGCGTGGAGACCAACGAAGCGTGTCCCTGGC ACACAGCAAGGGAGAGTTTCGCTCGGTCTCTGCAGTGCAGTCTCCTGGCGTAAGTCTTGAAGATGGACCCAACTCCCTGAAGAAATGGATCTTGAAATTG AACACAAACATCATGAACGTACAGCCTGGGCATTCTGCAGTGATTTGTGAGATGAGCTTGCATCGACTGTTCCTCTCAGCAGTGACAGCCAAAGTCACCC CTGATAAAATGCAGTTTCACTTTATAAAATGCCTCGCCCCTTCCCAGCATGTAGAGCTTGTCTGCAGCCCTAAAGGAGCCAGGTCCTGCTCTGATGAGGG TCAAATCAGAGCCGACATCCCATGTACAAAAAACCCCCAGGCTTCTGCCTTCCTTCCGGGCTCCTGTCTGCTCCCATTTTACTCCACGGAATAGTTCTGC CCTGTAGCATCTCCAGCGGCCTTTGCAGACTGCTGTCAGCCAACAGGTGCCAAAAATGCAAAAGAAGAAGAAACTAAAAGCAGTGCTATTTTTGCAGAAC AAATGCTAGCTATTAAATCATCTTAGGCCAAGACACCAATGGCACTTGGACCGATAGGTATTTGGAGCATGAGTTAGGAAGGATATGTCTTTCTCCAGGT GCGCAGCTGAAAGTTGCTGAGAAAAGCTGCCTGTGTTGCTGTGGTAACATGAGAAGGAATGAACAGCTCTATACAAAAACTGGCCACACATTATAAGGCC TATTCTATGCAGTTATGTTTTTGGCTATTTGCTCATTTAGTCATTGTTTATCAACCAAACTAGGTAAGCCAAGGAGTTACAAGACAGCTCTAGAGAAGCT CCCTCTGCTTACAGGACAATAATGCATTGTTTGTTTGTTGATACAAATGAATTATTTGAATCCACAGAAGTGAAAAAGGGAAAAAATCCTGGCTGACTTG TGGGAAGAGCGTACTGCAGAGCCTAGGGCCTGAGTTAACTCGCTGGCGGCCCTGCAGGCAGGCTTGATGGGAGCGCCCAGCACATCTGCCCACCCTGCAC AGGGCATGAAGCACCGTGTGCAGAGATCTGTGGGCAAAAGCCCAGGACGTGACCTTAGGCAAGAGGGGTCCTTGGAGAAGTGGAGGTGTGCATGGTGTGT AGCCTTGCGTGTCTGTGAGCACGCCCTAGTGGACCTGCGCCTGGATTATCGGGCCAGTCTCTCGGAGGGGTCCGTGATGCTGAGTTACGCCATTCACCTG AACTCTGCAGCTCATCGGCTGTAGGATTTGGGTGAGACACTTTTCCTTGCTGACTCATTTCCCCATCTGCAAAATGGGGGTGACGATATCTACCTCTTGG GGTGTGTCAAGGGTAAAAGAGCACACTTTAAGAATCCTAAAGCACTAAGCCAAGGATGAAAGACTAGAAAGCTGCCTCTATGTTGCTACTCTCAAAAATG CTCAGAAATTTTCTTGTCGTACTAAAAATGTGTTATTTTTCTCATTTTCATTCTTCCATTCAACACAGAAGTAATCTAAGTAAGTTCTAAAAACATAGAT CCGGGGGCCCACAGACATTCAATTTTCTTCTGAATTGGCTCTGGTATCACACAACACACAAGCAAGCAAACAGCCTGCCTGCTAGGGCCTTGGGTTCCAG GCTGGAGGAATGCAGGCAGAAATCTCTTAGGAAATAACTAAGTGAGCGCCAAGTTTTGAATTGCCTTAGAGCCGAAAGAGAATGTGGCGTGCTGGGGAGA GATCTGGACCTCTTTCTCATCAGTCATTTGACCTGTCCACGAAGAACAATTTTGTTATGCAGCCTGGGAACCTGGTTTCCTTACACCCTGCGCCCTTAAT GACACAGACAACCACGGGTACTCCTGGACGGAAGCTCATCACGCCTCCTCAAATACCTTCACCCATGTTCAGGTCACACGGAGGTCACCATCTCCTCAGC ACACAACAACTGCTTTGGTTCCCAGGGTTATTTTTTCTACGAGTTGAAAACCCACGGAGCCTAAGCCATCTTTTTGTCCCCTAAGGAGAGCCCCATAGAC TAAGAGCTACACTTCCTGTCTGTCTTTCCCACCTTTCCATCTCATTCTAGCTTAAAGCTTATCACGCTACTGAGAGCCTGTTCTGTAGTTCAAGGTGGCA GAGAAAAATACGAACCTTGAGTGAAATCTGGAGAGCAGTGCAGGGTCATCCCCAGACGTGACGGGCCATATCCTAGGCGACTCCTGCAGCTTATAGAAGC AGGGAATTTAATGGACTTCTTCCAGGAAAGCCAGCCCACGGGGACTCTTAAAAAGACACCAACCATTTGGAAGATGCAGCTTGAGGTCTGGGGGACTGGA CTGATTCTGCCACCATATAGTGAACACGATTAATCGGGAGGAAGAAGTAAGGCACTCTGTGAGTTAGTCTACCACCCTATTGACTTTGACAGAGAGAAAA CCAACTTTTTTCATATGATCACACAGTAGTACTGGAATATCTCGACTGCAATATCCTCTAAACAAATGCATGCAAAGCTTTGTAAAAGCAGCTGATGATT AAAAAAATTTTAAAACATTAGGGTTTTTCTAATATATCCATATGTGGGACAGTGATTTAAATGTCATTAATTGTCCAGCCACAACTTGTAACAGTGATGT GAAAGACACAAAAAAGGCCCCACTGGAGTGGAGGAGTGGTCTGGCTGGTCCCCAAGCAAAACAGAATGGGCTTCGCATAGGCTTTAGAACTTATTCATAA ATATTGACCAAAGTGTGAAGCAAGGTATCCAGTGAAGATTCTCTAGGGTCTCTACTTCTGCTCAAGAGATTTAATCCAGCTTTCTCTAAAGCAGTGGTCC AGACTTGCCACATACTAGGTTAAAGATAAAGTGAGAAAAACAAATACTTTGGTTTGCACGTCTCTTGAGGGGCCCTAGGCTGTATGGAGGAATATTTAGA GGAATATTTGTGAGCTGCCATTTGTGTTTAAAAGAACATGAAAGCAAGACGGGCCCTAACCCGCCTGTGTCTGTAGCTGACCAGGAGGCCACTGCTTCCT TCCCCCAGAGCTTCTAGGAGAGCCCCCCAAAATGCAACTCTCCAAGGTCCTGTGGAGTAGTCCCCTCATAATGCCTCCACCACACCTGTGTCTCACGATG CCTTAACTGACGTAGCTCCTAAGTGATGTTCACAGAGAAGGAAATACTTAGGCAAGCCCATGTGTAACATGCTCACAGATGTGCTGGCTTTGCCAATGCT TTCGGACTCCACCATGCTTTCTGATTCTTCTGGTCCTAGTCACGCCTTGGCACGGGGCAGAAGCGGCCGAGCTATGCTGACAGGTGCTGCTGAGAGCCAA CAGCCATTCAGCCGAGAAGGCGTTTCTAGGTGAGGGCATGAGAGCTGGCATTCCAGGCTGCTGAGTGCGGCATCTGCAGGTGCACCACATTGCTCCACGG TGTGTTCAGAAGAGACCAGCATCTTGAATCTCAGGCTCTGGGCTCATTCTTTGCCTCTGCCAAGGGACCATTCAGGGGCTTCAAGCCAGCTGCTGAAAAT CCCCCTTTTCTGGATTTTTCCCCATCTATATAAGGATATGGTGGCTGTGTGGACAAGAAATTGGTTTCATCTCAGAAGGGTGCTTCCCTACAAAGATCTT CAGAAATTTAATTGAAAACAATTTTTTGAACTTTTTTTGTTTGGCCAGGATGAATGAAGCTGAAGTCACATCCTGAATGTGCATGCCAGACAAAGGGTAA GCAAGCAGTCTTTACAAATGCCAATCCCTCTGCACTCCAAAACCGGAGCCCGTAATGAAGGCCCAGGTTCCAGTCATCACAGGATCCCCCAAGCCAGCAC AGTGCCTGATGCCTCCCTAGGCATTAGCACATCCCAGCACTCTAATTCACATCCTCAGCATCCCTGCCTCCTGCATCCAGGGCTTAGGTTGAGTGTTTCC AGAGTGTCAAGTACATTTAAACACAAACCAAATACTCCAGGGTTTTGACCTGTCACAATGGCTTTTGAAAACAACAGACGTACAATTTGAGAGAAGGACT CGATGGACTTCTTAGGCTGAGGACTAAGGACATGTTGCTGTTCCTCTTGGTCCAGGACATTCAATACCATTCACAGCATTCATCACCAACTGACATAGTA TCGATCACGACATTCAACTGTTTGAAATTACTGCTATTTTGGTCAAAAACTAAGAAATCAGAAATTGCACCTACATAGTGTGTCTCCTCATTGGAGTCCC ATCTCTCTGAGCACAGGGATGGGCTCCTGAGCTCACAGCCCTGCCCTGCCCCCCCAGCAGGGCCTCACCCTCATGGCCCTCAGTGAATAGTGACTACATG AAGGGGGCACTTACTAGAGGCCTGAGTAATGGAATTTCAATTAGCTTCTGCTTCCCTTTTCTAAAAAAGAACCACCTTTCTTAAGAATAATTAGGCCAAA ATAAACTTGAGTAATTTGTACAGTCAATATTTTGAAGTTTCGGAGTTACCAGTGAAATAGCAAAGCTGTTCTCCAGGAAAGGACATCCAGGTTCCCACCC AGGCCTTCCTTCCTCAGCTCCTGCAGCCTCATCCTAGTTTAATATGAAGGTTGTGAATGGGTTAAAAAGCAGTTCTTAAGCACCTACTTAGATCTTTTCA GTATCTGGAAATAGCACCCAGGGCTGTGTTAGTAAAGCTTTTCACTGGGTAAGAGTCATACAATTTTTACATTTAATTTGTTTTTTTAAAACAAGGCAGT CTTGTTTTACATATTGGAATGACTAGATATTTTTTGACATTTCTGGCACCTCTGGTTCTGCTGAATTTTAAGGGAGGTGAGTGGTATGTACACAAAGTTA AGATTGCTGCATAATAATTCCTTTGCATTTCCAAAATAAATTACACAAATGTCACTTTTCCTTTTCTATTCCATAGTACTAGTTTTAGCCAGATCTTTGC CAAACATTCTAGGACATTCAGAAAAGTCATCCATGGAATTTTGGAATGCCATTCTCTCTGTATCTTTGCAGATCATGAGGAAGGAAAGTTGATGAACTAG TACGGGATGGCTCAGACTTGTAAAGTGTCTAAGTCACAACGCTGCAGAGCCAGCTTCCGGGTAGCAATGGCATGGCCTCAGCTGTGCCAAGGCTACAACT GGAGCTTCCTTACTCGCATCTGAGGCAAACACAGCAAGGCTGGCCCAGGCCGCCCACAGTCAACAGATGTTCACGGGAGATGGATCAGCCCCTCTTTTGC TTGTTCTATCAAGGAAAGAGATCTAACCATGGGCAAGGGTGGAACACACCCGGTTCAGGTCAGGGAATGAGTTGCTCTAAGTGAACAGTGAATTCTTACT TCTCAGCCTGTATCAATTGTACCCAGGGTTCATGATTACTGTGACCCACTAAGGGATGATCTTTGAGGAAACAGACCTGGAGGGAGGTAATTTAAGGAGG GAAAGCGAGTGGGTTTTACAGACTGCTTTGTGGCGACATTCAGGAACAGACCATTCAATGGATGACTTTTCAGTACATAAAAGCAAGCAGTAATGGCCAG GAGAGAGCAAAGATGCCCTAAGAATGACATCAGACTGACCAAATGTGCCTTTTCAAATAAGTTATGAGAACGAATGAATTGGAATTAGAGCAAGGTATTG GACAGAACCATGCTAGTCTTTGAGAGAACGTGTGGATTGGGTGGATGTTTGGACCGAATGAATGGTAACTTTTTAATGTGAAACAGAGAACTGATATATG GGCTGTCATGTCACCCTAGGGTACTGTCGCTCTGTCATGTGTAGCAATCTTTCCAAATTTTTGTTGGTGGGCTGAACAAAGATAAAACTAATAAAGTATG TGTGGATACCACTAGA

refseq_AGPAT4.F1 (forward primer)
refseq_AGPAT4.R1 (reverse primer)
Single exon skipping

Frame 0

MGGVSLFLSSVG*LTALIN*WQFLNPAGSISAS GF*VHVPKAARKETPS*VLDLSSFWKSLTVGSYL FLNKSVHASWTSRDC*SLSSCATWSSATSLLPQ G*SSTPFSSSLSSSGPLTSSSSGRSTADCPIAS QASW*CCWSGGRARNAPSSRTRAPTSSMGRKMPS WFSTTSLKLTFCVAGACPNALGC*GLTSCT*LA CLWNWVSSVDFCRLQHTAACSG*ACVT*LNQSH LRYSGLQGPGQERAGLCPNYRLDVVLHRDGLLFA QVGAGSQDGCHQFAAPPGLPREVFFPDSL*GHT VHGEEA*DQHAGGPGQGAASPQASPVATNQGLR HHREELEKCSFSCI*LYTQFQK**KSNTAGSPKR KEIPCRFVC*EDPTGRHP*RR*RVLGLAAQALP GEGCLSGGVLQDGHLPRDAHGAPPAALDPRELA VLGLAGALPFLPVPGQHDQERVFPDAGQLHPRLL CGLRGSSMDDWCDGN*QGLCLRQL*QQAETE*L TQGGVTIRREPWGTGGLCISSLVGHGDKGWVSP CWARRKSRPLQPGSLVSRPDGEEDVL*SFFPHVL *WALVFFLCECV*EWLCGECELCSVIIERVF*A AGEGRAGDRRGQVPLSSFGAEFSVTLGCQR*SE KCFR*DD*IMPPRKKN*SAFLGHLWCMLTWTAAP PSCPCPWLWCFRSYSREAAAAEVWAAERGGQAS TAPGRRHRSRSP*AFVRAEMASTCVETNEACPW HTARESFARSLQCSLLA*VLKMDPTP*RNGS*N* TQTS*TYSLGILQ*FVR*ACIDCSSQQ*QPKSP LIKCSFTL*NASPLPSM*SLSAALKEPGPALMR VKSEPTSHVQKTPRLLPSFRAPVCSHFTPRNSSA L*HLQRPLQTAVSQQVPKMQKKKKLKAVLFLQN KC*LLNHLRPRHQWHLDR*VFGA*VRKDMSFSR CAAESC*EKLPVLLW*HEKE*TALYKNWPHIIRP ILCSYVFGYLLI*SLFINQTR*AKELQDSSREA PSAYRTIMHCLFVDTNELFESTEVKKGKNPG*L VGRAYCRA*GLS*LAGGPAGRLDGSAQHICPPCT GHEAPCAEICGQKPRT*P*ARGVLGEVEVCMVC SLACL*ARPSGPAPGLSGQSLGGVRDAELRHSP ELCSSSAVGFG*DTFPC*LISPSAKWG*RYLPLG VCQG*KSTL*ES*STKPRMKD*KAASMLLLSKM LRNFLVVLKMCYFSHFHSSIQHRSNLSKF*KHR SGGPQTFNFLLNWLWYHTTHKQANSLPARALGSR LEECRQKSLRK*LSERQVLNCLRAERECGVLGR DLDLFLISHLTCPRRTILLCSLGTWFPYTLRP* *HRQPRVLLDGSSSRLLKYLHPCSGHTEVTISSA HNNCFGSQGYFFYELKTHGA*AIFLSPKESPID *ELHFLSVFPTFPSHSSLKLITLLRACSVVQGG REKYEP*VKSGEQCRVIPRRDGPYPRRLLQLIEA GNLMDFFQESQPTGTLKKTPTIWKMQLEVWGTG LILPPYSEHD*SGGRSKALCELVYHPIDFDREK TNFFHMITQ*YWNISTAISSKQMHAKLCKSS**L KKF*NIRVFLIYPYVGQ*FKCH*LSSHNL*Q*C ERHKKGPTGVEEWSGWSPSKTEWASHRL*NLFI NIDQSVKQGIQ*RFSRVSTSAQEI*SSFL*SSGP DLPHTRLKIK*EKQILWFARLLRGPRLYGGIFR GIFVSCHLCLKEHESKTGPNPPVSVADQEATAS FPQSF*ESPPKCNSPRSCGVVPS*CLHHTCVSRC LN*RSS*VMFTEKEILRQAHV*HAHRCAGFANA FGLHHAF*FFWS*SRLGTGQKRPSYADRCC*EP TAIQPRRRF*VRA*ELAFQAAECGICRCTTLLHG VFRRDQHLESQALGSFFASAKGPFRGFKPAAEN PPFLDFSPSI*GYGGCVDKKLVSSQKGASLQRS SEI*LKTIF*TFFVWPG*MKLKSHPECACQTKGK QAVFTNANPSALQNRSP**RPRFQSSQDPPSQH SA*CLPRH*HIPAL*FTSSASLPPASRA*VECF QSVKYI*TQTKYSRVLTCHNGF*KQQTYNLREGL DGLLRLRTKDMLLFLLVQDIQYHSQHSSPTDIV SITTFNCLKLLLFWSKTKKSEIAPT*CVSSLES HLSEHRDGLLSSQPCPAPPAGPHPHGPQ*IVTT* RGHLLEA*VMEFQLASASLF*KRTTFLKNN*AK INLSNLYSQYFEVSELPVK*QSCSPGKDIQVPT QAFLPQLLQPHPSLI*RL*MG*KAVLKHLLRSFQ YLEIAPRAVLVKLFTG*ESYNFYI*FVFLKQGS LVLHIGMTRYFLTFLAPLVLLNFKGGEWYVHKV KIAA**FLCISKINYTNVTFPFLFHSTSFSQIFA KHSRTFRKVIHGILECHSLCIFADHEEGKLMN* YGMAQTCKVSKSQRCRASFRVAMAWPQLCQGYN WSFLTRI*GKHSKAGPGRPQSTDVHGRWISPSFA CSIKERDLTMGKGGTHPVQVRE*VALSEQ*ILT SQPVSIVPRVHDYCDPLRDDL*GNRPGGR*FKE GKRVGFTDCFVATFRNRPFNG*LFST*KQAVMAR REQRCPKNDIRLTKCAFSNKL*ERMNWN*SKVL DRTMLVFERTCGLGGCLDRMNGNFLM*NRELIY GLSCHPRVLSLCHV*QSFQIFVGGLNKDKTNKVC VDTTR

Frame 1

WVESRFFFPVLADLQLL*TSGNF*TQPAPSQLL VSKSMCQRLPGRRRLPESWIFLPSGNL*LWVVI YF*IRASTHHGPRGTAEVSVPVPPGLLLRLYCLR ANHQHHSALHSPPLAH*QAALPEDQLQTVLLHL KPAGDAAGVVVGHGMHHLHGPARLPQVWEGKCH RGSQPQV*N*LSVWLEPVRTLWAVRV*PLARSLH AFGTGSLQLTFVVFNTLQRVAVEPVSHS*IRVI CGTQGSKVLAKKELAYVPIIGWMWYFTEMVFCS RKWEQDRKTVATSLQHLRDYPEKYFFLIHCEGTR FTEKKHEISMQVARAKGLPRLKHHLLPRTKGFA ITVRSLRNVVSAVYDCTLNFRNNENPTLLGVLN GKKYHADLYVRRIPLEDIPEDDDECSAWLHKLYQ EKDAFQEEYYRTGTFPETPMVPPRRPWTLVNWL FWASLVLYPFFQFLVSMIRSGSSLTLASFILVF FVASVGVRWMIGVTEIDKGSAYGNSDSKQKLND* LREVSPSEGNLGELVASAYPP*WDTVTKAG*AP AGHGGSHDLSSQGVWSQGRMGRKMFCNLFFPMC FSGLWFSFCASVCENGCVVSVNFVL*S*KGYFRL QGRAGLGTEGDKFPFHPLVLSFL*PLVARDKVK SALGEMTKLCLQEKKIKVLFWVICGVC*HGPLP LHPALARGFGVSDPTHGRQLPRRCGQLRGVGRPA QLLAGDTGPGAREHL*EQRWQARAWRPTKRVPG TQQGRVSLGLCSAVSWRKS*RWTQLPEEMDLEI EHKHHERTAWAFCSDL*DELASTVPLSSDSQSHP **NAVSLYKMPRPFPACRACLQP*RSQVLL**G SNQSRHPMYKKPPGFCLPSGLLSAPILLHGIVL PCSISSGLCRLLSANRCQKCKRRRN*KQCYFCRT NASY*IILGQDTNGTWTDRYLEHELGRICLSPG AQLKVAEKSCLCCCGNMRRNEQLYTKTGHTL*G LFYAVMFLAICSFSHCLSTKLGKPRSYKTALEKL PLLTGQ*CIVCLLIQMNYLNPQK*KREKILADL WEERTAEPRA*VNSLAALQAGLMGAPSTSAHPA QGMKHRVQRSVGKSPGRDLRQEGSLEKWRCAWCV ALRVCEHALVDLRLDYRASLSEGSVMLSYAIHL NSAAHRL*DLGETLFLADSFPHLQNGGDDIYLL GCVKGKRAHFKNPKALSQG*KTRKLPLCCYSQKC SEIFLSY*KCVIFLIFILPFNTEVI*VSSKNID PGAHRHSIFF*IGSGITQHTSKQTACLLGPWVP GWRNAGRNLLGNN*VSAKF*IALEPKENVACWGE IWTSFSSVI*PVHEEQFCYAAWEPGFLTPCALN DTDNHGYSWTEAHHASSNTFTHVQVTRRSPSPQ HTTTALVPRVIFSTS*KPTEPKPSFCPLRRAP*T KSYTSCLSFPPFHLILA*SLSRY*EPVL*FKVA EKNTNLE*NLESSAGSSPDVTGHILGDSCSL*K QGI*WTSSRKASPRGLLKRHQPFGRCSLRSGGLD *FCHHIVNTINREEEVRHSVS*STTLLTLTERK PTFFI*SHSSTGISRLQYPLNKCMQSFVKAADD *KNFKTLGFF*YIHMWDSDLNVINCPATTCNSDV KDTKKAPLEWRSGLAGPQAKQNGLRIGFRTYS* ILTKV*SKVSSEDSLGSLLLLKRFNPAFSKAVV QTCHILG*R*SEKNKYFGLHVS*GALGCMEEYLE EYL*AAICV*KNMKARRALTRLCL*LTRRPLLP SPRASRRAPQNATLQGPVE*SPHNASTTPVSHD ALTDVAPK*CSQRRKYLGKPMCNMLTDVLALPML SDSTMLSDSSGPSHALARGRSGRAMLTGAAESQ QPFSREGVSR*GHESWHSRLLSAASAGAPHCST VCSEETSILNLRLWAHSLPLPRDHSGASSQLLKI PLFWIFPHLYKDMVAVWTRNWFHLRRVLPYKDL QKFN*KQFFELFLFGQDE*S*SHILNVHARQRV SKQSLQMPIPLHSKTGARNEGPGSSHHRIPQAST VPDASLGISTSQHSNSHPQHPCLLHPGLRLSVS RVSSTFKHKPNTPGF*PVTMAFENNRRTI*EKD SMDFLG*GLRTCCCSSWSRTFNTIHSIHHQLT*Y RSRHSTV*NYCYFGQKLRNQKLHLHSVSPHWSP ISLSTGMGS*AHSPALPPQQGLTLMALSE**LH EGGTY*RPE*WNFN*LLLPFSKKEPPFLRIIRPK *T*VICTVNILKFRSYQ*NSKAVLQERTSRFPP RPSFLSSCSLILV*YEGCEWVKKQFLSTYLDLF SIWK*HPGLC**SFSLGKSHTIFTFNLFF*NKAV LFYILE*LDIF*HFWHLWFC*ILREVSGMYTKL RLLHNNSFAFPK*ITQMSLFLFYSIVLVLARSL PNILGHSEKSSMEFWNAILSVSLQIMRKES**TS TGWLRLVKCLSHNAAEPASG*QWHGLSCAKATT GASLLASEANTARLAQAAHSQQMFTGDGSAPLL LVLSRKEI*PWARVEHTRFRSGNELL*VNSEFLL LSLYQLYPGFMITVTH*GMIFEETDLEGGNLRR ESEWVLQTALWRHSGTDHSMDDFSVHKSKQ*WP GESKDALRMTSD*PNVPFQISYENE*IGIRARYW TEPC*SLRERVDWVDVWTE*MVTF*CETEN*YM GCHVTLGYCRSVMCSNLSKFLLVG*TKIKLIKY VWIPL

Frame 2

GWSLAFSFQCWLTYSSYKLVAISEPSRLHLSFW FLSPCAKGCQEGDAFLSPGSFFLLEIFDCG*LF ISE*ERPRIMDLAGLLKSQFLCHLVFCYVFIAS GLIINTIQLFTLLLWPINKQLFRKINCRLSYCIS SQLVMLLEWWSGTECTIFTDPRAYLKYGKENAI VVLNHKFEIDFLCGWSLSERFGLLGFDLLHVAC MPLELGLFS*LLSSSTHCSV*RLSLCHIAESESS AVLRAPRSWPRKSWPMSQLSAGCGTSPRWSSVR ASGSRIARRLPPVCSTSGTTPRSIFS*FTVRAH GSRRRSMRSACRWPGPRGCLASSITCCHEPRASP SP*GA*EM*FQLYMTVHSISEIMKIQHCWES*T ERNTMQICMLGGSHWKTSLKTMTSARPGCTSST RRRMPFRRSTTGRAPSQRRPWCPPGGPGPS*TGC FGPRWCSTLSSSSWSA*SGAGLP*RWPASSSSS LWPPWEFDG*LV*RKLTRALPTATLTASRN*MT DSGRCHHPKGTLGNWWPLHILLSGTR*QRLGEPL LGTAEVTTSPARESGLKAGWGGRCFVIFFSPCA LVGFGFLFVRVCVRMAVW*V*TLFCDHRKGILG CRGGQGWGPKGTSSPFILWC*VFCNPWLPEIK*K VL*VR*LNYASKKKKLKCFSGSSVVYVDMDRCP SILPLPVALVFQILLTGGSCRGGVGS*EGWAGQ HSSWQETQVQEPVSICESRDGKHVRGDQRSVSLA HSKGEFRSVSAVQSPGVSLEDGPNSLKKWILKL NTNIMNVQPGHSAVICEMSLHRLFLSAVTAKVT PDKMQFHFIKCLAPSQHVELVCSPKGARSCSDEG QIRADIPCTKNPQASAFLPGSCLLPFYSTE*FC PVASPAAFADCCQPTGAKNAKEEETKSSAIFAE QMLAIKSS*AKTPMALGPIGIWSMS*EGYVFLQV RS*KLLRKAACVAVVT*EGMNSSIQKLATHYKA YSMQLCFWLFAHLVIVYQPN*VSQGVTRQL*RS SLCLQDNNALFVC*YK*II*IHRSEKGKKSWLTC GKSVLQSLGPELTRWRPCRQA*WERPAHLPTLH RA*STVCRDLWAKAQDVTLGKRGPWRSGGVHGV *PCVSVSTP*WTCAWIIGPVSRRGP*C*VTPFT* TLQLIGCRIWVRHFSLLTHFPICKMGVTISTSW GVSRVKEHTLRILKH*AKDERLESCLYVATLKN AQKFSCRTKNVLFFSFSFFHSTQK*SK*VLKT*I RGPTDIQFSSELALVSHNTQASKQPAC*GLGFQ AGGMQAEIS*EITK*APSFELP*SRKRMWRAGE RSGPLSHQSFDLSTKNNFVMQPGNLVSLHPAPLM TQTTTGTPGRKLITPPQIPSPMFRSHGGHHLLS TQQLLWFPGLFFLRVENPRSLSHLFVP*GEPHR LRATLPVCLSHLSISF*LKAYHATESLFCSSRWQ RKIRTLSEIWRAVQGHPQT*RAIS*ATPAAYRS REFNGLLPGKPAHGDS*KDTNHLEDAA*GLGDW TDSATI**TRLIGRKK*GTL*VSLPPY*L*QREN QLFSYDHTVVLEYLDCNIL*TNACKAL*KQLMI KKILKH*GFSNISICGTVI*MSLIVQPQLVTVM *KTQKRPHWSGGVVWLVPKQNRMGFA*ALELIHK Y*PKCEARYPVKIL*GLYFCSRDLIQLSLKQWS RLATY*VKDKVRKTNTLVCTSLEGP*AVWRNI* RNICELPFVFKRT*KQDGP*PACVCS*PGGHCFL PPELLGEPPKMQLSKVLWSSPLIMPPPHLCLTM P*LT*LLSDVHREGNT*ASPCVTCSQMCWLCQC FRTPPCFLILLVLVTPWHGAEAAELC*QVLLRAN SHSAEKAFLGEGMRAGIPGC*VRHLQVHHIAPR CVQKRPAS*ISGSGLILCLCQGTIQGLQASC*K SPFSGFFPIYIRIWWLCGQEIGFISEGCFPTKIF RNLIENNFLNFFCLARMNEAEVTS*MCMPDKG* ASSLYKCQSLCTPKPEPVMKAQVPVITGSPKPA QCLMPP*ALAHPSTLIHILSIPASCIQGLG*VFP ECQVHLNTNQILQGFDLSQWLLKTTDVQFERRT RWTS*AED*GHVAVPLGPGHSIPFTAFITN*HS IDHDIQLFEITAILVKN*EIRNCTYIVCLLIGVP SL*AQGWAPELTALPCPPSRASPSWPSVNSDYM KGALTRGLSNGISISFCFPFLKKNHLS*E*LGQ NKLE*FVQSIF*SFGVTSEIAKLFSRKGHPGSHP GLPSSAPAASS*FNMKVVNGLKSSS*APT*IFS VSGNSTQGCVSKAFHWVRVIQFLHLICFFKTRQ SCFTYWND*IFFDISGTSGSAEF*GR*VVCTQS* DCCIIIPLHFQNKLHKCHFSFSIP*Y*F*PDLC QTF*DIQKSHPWNFGMPFSLYLCRS*GRKVDEL VRDGSDL*SV*VTTLQSQLPGSNGMASAVPRLQL ELPYSHLRQTQQGWPRPPTVNRCSREMDQPLFC LFYQGKRSNHGQGWNTPGSGQGMSCSK*TVNSY FSACINCTQGS*LL*PTKG*SLRKQTWREVI*GG KASGFYRLLCGDIQEQTIQWMTFQYIKASSNGQ ERAKMP*E*HQTDQMCLFK*VMRTNELELEQGI GQNHASL*ENVWIGWMFGPNEW*LFNVKQRTDIW AVMSP*GTVALSCVAIFPNFCWWAEQR*N**SM CGYH*