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Sequences

Sequence NM_007222

ACAGGTCAGAGTTGACTCCCTGAAAAGTGCAGCCGGTTTGAAATGCAAGATGGCGGCGGCGTGGCGCTGAGAGGCGCGGCGGCCCCTGCAGGAGAAGACA
GACTGCTGCTTTGGACCTGTTGGTAATGATGGCCTGAGCTAAACATCTAACTAGAAGGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGCTGT
GGCAAGTGATTGGAGTTGTGCTTCAAAAATTTCAGAAATTCAGCAGTATTTTATCTGCCAACAATAAGCTCTTTACTTGATTGCACCATGAGAAAGCTGC
TAATGAGACTTGTTGAGCACAAAAATGGACTTGAAGAACCAAAAGCCATTGTTTTCAAATGAAGAACACTGAACAGTTTTAAGCCTCGATGCTTTTTAAT
CACCACTGAGCTTTTCCTCATAACATCAGAATGGCAAGCAGGCGAAAATCAACAACACCTTGCATGGTCCTTGCCAGTGAACAAGATCCAGACCTTGAGT
TGATATCAGATTTGGATGAAGGTCCTCCTGTGCTTACACCTGTAGAAAACACCAGAGCAGAGAGTATCTCAAGTGATGAAGAGGTTCATGAATCTGTGGA
TTCAGACAATCAGCAAAATAAAAAAGTTGAAGGTGGATATGAATGTAAATATTGTACTTTTCAAACTCCAGATCTAAATATGTTTACTTTTCATGTGGAT
TCGGAACATCCCAATGTAGTGCTAAATTCATCCTATGTTTGTGTCGAATGCAATTTTCTTACCAAAAGGTATGATGCACTTTCTGAGCATAATCTGAAAT
ATCACCCAGGAGAAGAGAATTTTAAGTTGACTATGGTGAAACGTAATAACCAGACAATCTTTGAACAAACAATAAATGATCTGACTTTTGATGGTAGTTT
TGTTAAAGAGGAGAATGCAGAGCAAGCAGAATCTACAGAAGTTTCTTCTTCGGGAATATCTATCAGTAAAACTCCTATCATGAAAATGATGAAAAATAAA
GTGGAAAATAAACGGATTGCAGTTCATCATAACTCAGTTGAGGACGTTCCTGAAGAGAAAGAGAATGAAATCAAACCAGACCGTGAAGAAATTGTAGAAA
ATCCAAGTTCTTCAGCTTCTGAATCTAATACAAGTACTTCCATTGTAAACAGAATACATCCAAGTACTGCCAGCACGGTAGTGACACCAGCAGCAGTTCT
TCCTGGATTGGCACAGGTGATAACTGCTGTATCTGCTCAGCAGAATTCTAATTTGATTCCCAAAGTCTTAATCCCTGTTAATAGCATTCCCACCTACAAT
GCTGCATTGGATAACAATCCCCTTTTACTTAACACCTACAACAAGTTCCCTTACCCAACAATGTCAGAAATTACAGTTCTTTCTGCTCAAGCAAAATATA
CAGAGGAACAGATCAAGATATGGTTTTCAGCCCAACGTTTAAAACATGGTGTTAGTTGGACTCCCGAGGAAGTAGAGGAGGCAAGAAGGAAACAATTCAA
TGGAACAGTGCATACTGTACCTCAGACCATAACTGTTATTCCTACACACATTTCCACAGGGAGTAATGGTTTACCATCTATTTTACAGACATGCCAAATA
GTTGGTCAGCCTGGTCTGGTCCTTACTCAAGTGGCTGGAACAAACACCTTGCCAGTTACAGCACCTATAGCCTTGACAGTGGCAGGCGTTCCAAGTCAAA
ATAATATACAGAAAAGTCAGGTACCTGCTGCTCAGCCTACTGCAGAAACAAAGCCAGCAACAGCAGCAGTTCCAACTTCTCAAAGTGTCAAACATGAAAC
TGCATTGGTAAACCCTGATTCATTTGGCATTCGGGCAAAAAAGACAAAAGAGCAACTGGCAGAATTAAAAGTTAGCTACCTTAAAAATCAGTTTCCCCAT
GATTCAGAAATTATCAGACTTATGAAAATAACAGGCCTGACGAAAGGAGAGATTAAAAAATGGTTTAGTGACACAAGGTACAACCAGAGAAATTCAAAGA
GTAATCAGTGCTTACATCTCAACAATGATTCCTCTACCACCATTATTATAGACTCCAGTGATGAAACCACGGAATCCCCAACTGTTGGTACTGCACAGCC
TAAGCAATCCTGGAATCCTTTTCCTGACTTTACTCCCCAAAAGTTTAAAGAGAAAACTGCAGAGCAGCTTCGTGTCCTTCAGGCAAGTTTTCTCAACAGC
TCTGTACTTACAGATGAAGAATTAAATAGGTTAAGGGCACAAACCAAACTTACCAGAAGAGAAATCGATGCTTGGTTTACAGAGAAGAAGAAATCAAAAG
CTTTAAAGGAAGAGAAAATGGAAATAGATGAAAGTAATGCAGGTAGTTCCAAAGAAGAAGCTGGAGAAACTTCTCCTGCAGATGAATCTGGTGCACCTAA
GTCAGGGAGTACAGGCAAGATATGTAAAAAAACACCTGAGCAGCTGCACATGCTTAAGAGTGCATTTGTCCGGACACAGTGGCCATCACCAGAAGAGTAT
GACAAGTTGGCCAAAGAAAGCGGGCTTGCTAGAACAGACATAGTTAGTTGGTTTGGGGACACCCGTTATGCTTGGAAGAATGGAAACTTGAAATGGTACT
ACTACTATCAGAGCGCCAATTCAAGTAGTATGAATGGTCTGTCTTCCCTTAGGAAAAGAGGGAGAGGGAGACCCAAAGGACGGGGAAGAGGAAGACCGCG
TGGGCGGCCTAGAGGAAGCAAAAGAATTAACAACTGGGACAGGGGACCATCACTCATAAAATTTAAAACTGGAACTGCAATACTTAAGGATTATTACCTG
AAGCACAAGTTTCTTAATGAGCAAGACCTTGATGAACTTGTTAACAAATCACATATGGGCTATGAGCAGGTCAGAGAGTGGTTTGCAGAAAGACAGAGAA
GATCAGAATTAGGTATAGAATTATTTGAGGAAAATGAGGAGGAAGATGAAGTTATTGATGACCAGGAAGAGGATGAAGAAGAAACAGATGATAGTGACAC
TTGGGAACCTCCACGACATGTGAAACGGAAGCTGTCTAAATCAGATGACTGAAATCTGCCTAAAACGTTGAAGGAGAATCAATTCTTCAACTCAAGATGT
CTGATTTACTGTGAATTTGCCCAATCTTTGATGACATTGAAAACGTTTTGGGGCATACACACTCAAAAAGCAGGATCCAATACCCAAAAGAAATGGAACT
TAATGTTGTGCCAAAGTTAAACTACTGCAGTTGGTGGAAGTTCTGCAATGTAAATAGAACACTAATTAAAAAACAACTTGTAAAAATGCAATTTAAATTT
TAATACAGTACATTTTTCTTCTAATATGATGGAGACATTCTGAATCTTAGACTTTCTGAGGGGGTTTAATGACCACTAGAGCTTGTCCTCATATTCAGTC
CAGTTTAATACTGTATGTCTAGTAAGATGGGCTATATATTGCCTCTATTCTTTGAGATGTGATTAAGCTTAGAACTTTGACCAAAAATTTTACAGTAAAA
ATTGTTAGAAATGGGTAAAAAAAAAAAAAAAAAAAAAACCCTGATTTATTACTATGTCTTATTTATCAGACCCTGCACTAAGATTAAAAGTTGTGCATGG
GCTTATACAATTTCAAATGTAAAAACAGCCTATTTATATATGTTGAAACAATAAGATTTTATGGTTTCACTGGCCCTTAATAATTGGATCCTTCATTAAA
CACATCCATGAATACCACTGTTTCTACTGTTACTTGTTCCATTAGAAGCTGATTTGCTTTTTATTAAACTGCAGGAAATTAATTCTGAAGAAGGAAAAGA
AACTGACATGGTAATTTGGATAGGGTGAAGCTTTGAAATAAATACTGCACCAAAAATGTGAAAAAGAATCACGTAGTAAATGTATACTTAGTGTTTTCTT
ACAATCGTGGAAATCAGAAATGTTTATACAGGTGTCAGATCAGAATTTTTAAGAGTTATCTTTCAGTGAAATTTTATAACAGTTTCAGTGAATAGAGAAA
AAATTCTGAACTGATCATAATGAGTTAAAAGTTAGCTTTTCACTTATGAATCATGGGAGTGTTAACTGTTAACTCTTGCCGTATCAGGAGACTGAAAGTT
CATACTAATTTACAAACTTTGTAAAGACTGGTGCTGTTCATTCATATTGGGAAGAACCCTTTCTTTTGTGACCATAGGACCATTTTTCAAAATGCGCTTT
CTTCGTAGAAGAAATGTTCAGTTTAATTAGCTTTGGATTTTAACATGTTTTTCTGAATGACCAGATGTAGGCTTAGCCAGTTTATTTTCTCTAATTTAAT
GGATAATAAATATTAAGCTCTTGGTTAAATGTTTTTCAGCCATTGAAAATGTTATGAAAAAGCCTTGAATATGCATGCTGCTTTCATTTTTATAAACTTT
TTATTTTTTAACTATAGCTTTATTAGTAATTCCAAAATGCTGCAATGTAGCTGATTTCCTCACTCAGACAGCTGGCTTTGTGGGTGGCTTTTTTCATACT
ACTGTTAACTTTTTTAAAAAAGAAGCAATGTAAAGACCGTAATGCACTAAGCTTTTTTTTTTTCTTTTACACGTTTAAAAACTTCTTAAAGCACTCTGTA
ATATAATGATTAAATTGCTCCCTTTTATAAACATTGCTAATGTGGTCTGAGTTTTGTTTGACAATAATTTTAATGTTCTCAAAATTTGAAATGTCAAGTA
TTAGAAATGAAGCCTTTGAATATAGTTGTTTCCCTTGCAATATGTTAACTCTGTGTAGCTGTATATAACTATTAATTTCCCTCATCTCTTTTATAGGCCT
GATAAAACTCTTCTCTGACGTTTGCTAGCTTTTAAATATATTTATTTGACAGAGCAAAAGAACCCTCTTCTGGTTGATTGTGACCTTGAATACAAAATAA
AAGTAGTGATTAGTAGTTTA
  • refseq_ZHX1.F1 (forward primer)
  • refseq_ZHX1.R1 (reverse primer)
  • Alternative donor

Frame 0

TGQS*LPEKCSRFEMQDGGGVALRGAAAPAGED
RLLLWTCW**WPELNI*LEGIPFHFKEQNAKEAV
ASDWSCASKISEIQQYFICQQ*ALYLIAP*ESC
**DLLSTKMDLKNQKPLFSNEEH*TVLSLDAF*
SPLSFSS*HQNGKQAKINNTLHGPCQ*TRSRP*V
DIRFG*RSSCAYTCRKHQSREYLK**RGS*ICG
FRQSAK*KS*RWI*M*ILYFSNSRSKYVYFSCG
FGTSQCSAKFILCLCRMQFSYQKV*CTF*A*SEI
SPRRREF*VDYGET**PDNL*TNNK*SDF*W*F
C*RGECRASRIYRSFFFGNIYQ*NSYHENDEK*
SGK*TDCSSS*LS*GRS*RERE*NQTRP*RNCRK
SKFFSF*I*YKYFHCKQNTSKYCQHGSDTSSSS
SWIGTGDNCCICSAEF*FDSQSLNPC**HSHLQ
CCIG*QSPFT*HLQQVPLPNNVRNYSSFCSSKIY
RGTDQDMVFSPTFKTWC*LDSRGSRGGKKETIQ
WNSAYCTSDHNCYSYTHFHRE*WFTIYFTDMPN
SWSAWSGPYSSGWNKHLASYSTYSLDSGRRSKSK
*YTEKSGTCCSAYCRNKASNSSSSNFSKCQT*N
CIGKP*FIWHSGKKDKRATGRIKS*LP*KSVSP
*FRNYQTYENNRPDERRD*KMV**HKVQPEKFKE
*SVLTSQQ*FLYHHYYRLQ**NHGIPNCWYCTA
*AILESFS*LYSPKV*RENCRAASCPSGKFSQQ
LCTYR*RIK*VKGTNQTYQKRNRCLVYREEEIKS
FKGRENGNR*K*CR*FQRRSWRNFSCR*IWCT*
VREYRQDM*KNT*AAAHA*ECICPDTVAITRRV
*QVGQRKRAC*NRHS*LVWGHPLCLEEWKLEMVL
LLSERQFK*YEWSVFP*EKRERETQRTGKRKTA
WAA*RKQKN*QLGQGTITHKI*NWNCNT*GLLP
EAQVS**ARP**TC*QITYGL*AGQRVVCRKTEK
IRIRYRII*GK*GGR*SY**PGRG*RRNR***H
LGTSTTCETEAV*IR*LKSA*NVEGESILQLKM
SDLL*ICPIFDDIENVLGHTHSKSRIQYPKEMEL
NVVPKLNYCSWWKFCNVNRTLIKKQLVKMQFKF
*YSTFFF*YDGDILNLRLSEGV**PLELVLIFS
PV*YCMSSKMGYILPLFFEM*LSLEL*PKILQ*K
LLEMGKKKKKKKTLIYYYVLFIRPCTKIKSCAW
AYTISNVKTAYLYMLKQ*DFMVSLALNNWILH*
THP*IPLFLLLLVPLEADLLFIKLQEINSEEGKE
TDMVIWIG*SFEINTAPKM*KRIT**MYT*CFL
TIVEIRNVYTGVRSEFLRVIFQ*NFITVSVNRE
KILN*S**VKS*LFTYESWEC*LLTLAVSGD*KF
ILIYKLCKDWCCSFILGRTLSFVTIGPFFKMRF
LRRRNVQFN*LWILTCFSE*PDVGLASLFSLI*
WIINIKLLVKCFSAIENVMKKP*ICMLLSFL*TF
YFLTIALLVIPKCCNVADFLTQTAGFVGGFFHT
TVNFFKKEAM*RP*CTKLFFFLLHV*KLLKALC
NIMIKLLPFINIANVV*VLFDNNFNVLKI*NVKY
*K*SL*I*LFPLQYVNSV*LYITINFPHLFYRP
DKTLL*RLLAFKYIYLTEQKNPLLVDCDLEYKI
KVVISSL

Frame 1

QVRVDSLKSAAGLKCKMAAAWR*EARRPLQEKT
DCCFGPVGNDGLS*TSN*KGYPSISKNRMLRKL
WQVIGVVLQKFQKFSSILSANNKLFT*LHHEKAA
NETC*AQKWT*RTKSHCFQMKNTEQF*ASMLFN
HH*AFPHNIRMASRRKSTTPCMVLASEQDPDLE
LISDLDEGPPVLTPVENTRAESISSDEEVHESVD
SDNQQNKKVEGGYECKYCTFQTPDLNMFTFHVD
SEHPNVVLNSSYVCVECNFLTKRYDALSEHNLK
YHPGEENFKLTMVKRNNQTIFEQTINDLTFDGSF
VKEENAEQAESTEVSSSGISISKTPIMKMMKNK
VENKRIAVHHNSVEDVPEEKENEIKPDREEIVE
NPSSSASESNTSTSIVNRIHPSTASTVVTPAAVL
PGLAQVITAVSAQQNSNLIPKVLIPVNSIPTYN
AALDNNPLLLNTYNKFPYPTMSEITVLSAQAKY
TEEQIKIWFSAQRLKHGVSWTPEEVEEARRKQFN
GTVHTVPQTITVIPTHISTGSNGLPSILQTCQI
VGQPGLVLTQVAGTNTLPVTAPIALTVAGVPSQ
NNIQKSQVPAAQPTAETKPATAAVPTSQSVKHET
ALVNPDSFGIRAKKTKEQLAELKVSYLKNQFPH
DSEIIRLMKITGLTKGEIKKWFSDTRYNQRNSK
SNQCLHLNNDSSTTIIIDSSDETTESPTVGTAQP
KQSWNPFPDFTPQKFKEKTAEQLRVLQASFLNS
SVLTDEELNRLRAQTKLTRREIDAWFTEKKKSK
ALKEEKMEIDESNAGSSKEEAGETSPADESGAPK
SGSTGKICKKTPEQLHMLKSAFVRTQWPSPEEY
DKLAKESGLARTDIVSWFGDTRYAWKNGNLKWY
YYYQSANSSSMNGLSSLRKRGRGRPKGRGRGRPR
GRPRGSKRINNWDRGPSLIKFKTGTAILKDYYL
KHKFLNEQDLDELVNKSHMGYEQVREWFAERQR
RSELGIELFEENEEEDEVIDDQEEDEEETDDSDT
WEPPRHVKRKLSKSDD*NLPKTLKENQFFNSRC
LIYCEFAQSLMTLKTFWGIHTQKAGSNTQKKWN
LMLCQS*TTAVGGSSAM*IEH*LKNNL*KCNLNF
NTVHFSSNMMETF*ILDFLRGFNDH*SLSSYSV
QFNTVCLVRWAIYCLYSLRCD*A*NFDQKFYSK
NC*KWVKKKKKKKP*FITMSYLSDPALRLKVVHG
LIQFQM*KQPIYIC*NNKILWFHWPLIIGSFIK
HIHEYHCFYCYLFH*KLICFLLNCRKLILKKEK
KLTW*FG*GEALK*ILHQKCEKESRSKCILSVFL
QSWKSEMFIQVSDQNF*ELSFSEIL*QFQ*IEK
KF*TDHNELKVSFSLMNHGSVNC*LLPYQETES
SY*FTNFVKTGAVHSYWEEPFLL*P*DHFSKCAF
FVEEMFSLISFGF*HVFLNDQM*A*PVYFL*FN
G**ILSSWLNVFQPLKML*KSLEYACCFHFYKL
FIF*L*LY**FQNAAM*LISSLRQLALWVAFFIL
LLTFLKKKQCKDRNALSFFFFFYTFKNFLKHSV
I**LNCSLL*TLLMWSEFCLTIILMFSKFEMSS
IRNEAFEYSCFPCNMLTLCSCI*LLISLISFIGL
IKLFSDVC*LLNIFI*QSKRTLFWLIVTLNTK*
K**LVV

Frame 2

RSELTP*KVQPV*NARWRRRGAERRGGPCRRRQ
TAALDLLVMMA*AKHLTRRDTLPFQRTEC*GSC
GK*LELCFKNFRNSAVFYLPTISSLLDCTMRKL
LMRLVEHKNGLEEPKAIVFK*RTLNSFKPRCFLI
TTELFLITSEWQAGENQQHLAWSLPVNKIQTLS
*YQIWMKVLLCLHL*KTPEQRVSQVMKRFMNLW
IQTISKIKKLKVDMNVNIVLFKLQI*ICLLFMWI
RNIPM*C*IHPMFVSNAIFLPKGMMHFLSII*N
ITQEKRILS*LW*NVITRQSLNKQ*MI*LLMVV
LLKRRMQSKQNLQKFLLREYLSVKLLS*K**KIK
WKINGLQFIITQLRTFLKRKRMKSNQTVKKL*K
IQVLQLLNLIQVLPL*TEYIQVLPAR**HQQQF
FLDWHR**LLYLLSRILI*FPKS*SLLIAFPPTM
LHWITIPFYLTPTTSSLTQQCQKLQFFLLKQNI
QRNRSRYGFQPNV*NMVLVGLPRK*RRQEGNNS
MEQCILYLRP*LLFLHTFPQGVMVYHLFYRHAK*
LVSLVWSLLKWLEQTPCQLQHL*P*QWQAFQVK
IIYRKVRYLLLSLLQKQSQQQQQFQLLKVSNMK
LHW*TLIHLAFGQKRQKSNWQN*KLATLKISFPM
IQKLSDL*K*QA*RKERLKNGLVTQGTTREIQR
VISAYISTMIPLPPLL*TPVMKPRNPQLLVLHS
LSNPGILFLTLLPKSLKRKLQSSFVSFRQVFSTA
LYLQMKN*IG*GHKPNLPEEKSMLGLQRRRNQK
L*RKRKWK*MKVMQVVPKKKLEKLLLQMNLVHL
SQGVQARYVKKHLSSCTCLRVHLSGHSGHHQKSM
TSWPKKAGLLEQT*LVGLGTPVMLGRMET*NGT
TTIRAPIQVV*MVCLPLGKEGEGDPKDGEEEDR
VGGLEEAKELTTGTGDHHS*NLKLELQYLRIIT*
STSFLMSKTLMNLLTNHIWAMSRSESGLQKDRE
DQN*V*NYLRKMRRKMKLLMTRKRMKKKQMIVT
LGNLHDM*NGSCLNQMTEICLKR*RRINSSTQDV
*FTVNLPNL**H*KRFGAYTLKKQDPIPKRNGT
*CCAKVKLLQLVEVLQCK*NTN*KTTCKNAI*I
LIQYIFLLI*WRHSES*TF*GGLMTTRACPHIQS
SLILYV**DGLYIASIL*DVIKLRTLTKNFTVK
IVRNG*KKKKKKNPDLLLCLIYQTLH*D*KLCM
GLYNFKCKNSLFIYVETIRFYGFTGP**LDPSLN
TSMNTTVSTVTCSIRS*FAFY*TAGN*F*RRKR
N*HGNLDRVKL*NKYCTKNVKKNHVVNVYLVFS
YNRGNQKCLYRCQIRIFKSYLSVKFYNSFSE*RK
NSELIIMS*KLAFHL*IMGVLTVNSCRIRRLKV
HTNLQTL*RLVLFIHIGKNPFFCDHRTIFQNAL
SS*KKCSV*LALDFNMFF*MTRCRLSQFIFSNLM
DNKY*ALG*MFFSH*KCYEKALNMHAAFIFINF
LFFNYSFISNSKMLQCS*FPHSDSWLCGWLFSY
YC*LF*KRSNVKTVMH*AFFFSFTRLKTS*STL*
YND*IAPFYKHC*CGLSFV*Q*F*CSQNLKCQV
LEMKPLNIVVSLAIC*LCVAVYNY*FPSSLL*A
**NSSLTFASF*IYLFDRAKEPSSG*L*P*IQNK
SSD**F

Sequence NM_001017926

ACAGGTCAGAGTTGACTCCCTGAAAAGTGCAGCCGGTTTGAAATGCAAGATGGCGGCGGCGTGGCGCTGAGAGGCGCGGCGGCCCCTGCAGGTAGTGGCG
CGAGCCGGCTCCGCGACCAGCGGGCCCGGAGACTCTGGGCTGCGGGTGGGAACTGGAGCGCAGAGTCCGCGGCCGGCACGGGAAAG
GAGAAGACAGACTG
CTGCTTTGGACCTGTTGGTAATGATGGCCTGAGCTAAACATCTAACTAGAAGGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGCTGTGGCAA
GTGATTGGAGTTGTGCTTCAAAAATTTCAGAAATTCAGCAGTATTTTATCTGCCAACAATAAGCTCTTTACTTGATTGCACCATGAGAAAGCTGCTAATG
AGACTTGTTGAGCACAAAAATGGACTTGAAGAACCAAAAGCCATTGTTTTCAAATGAAGAACACTGAACAGTTTTAAGCCTCGATGCTTTTTAATCACCA
CTGAGCTTTTCCTCATAACATCAGAATGGCAAGCAGGCGAAAATCAACAACACCTTGCATGGTCCTTGCCAGTGAACAAGATCCAGACCTTGAGTTGATA
TCAGATTTGGATGAAGGTCCTCCTGTGCTTACACCTGTAGAAAACACCAGAGCAGAGAGTATCTCAAGTGATGAAGAGGTTCATGAATCTGTGGATTCAG
ACAATCAGCAAAATAAAAAAGTTGAAGGTGGATATGAATGTAAATATTGTACTTTTCAAACTCCAGATCTAAATATGTTTACTTTTCATGTGGATTCGGA
ACATCCCAATGTAGTGCTAAATTCATCCTATGTTTGTGTCGAATGCAATTTTCTTACCAAAAGGTATGATGCACTTTCTGAGCATAATCTGAAATATCAC
CCAGGAGAAGAGAATTTTAAGTTGACTATGGTGAAACGTAATAACCAGACAATCTTTGAACAAACAATAAATGATCTGACTTTTGATGGTAGTTTTGTTA
AAGAGGAGAATGCAGAGCAAGCAGAATCTACAGAAGTTTCTTCTTCGGGAATATCTATCAGTAAAACTCCTATCATGAAAATGATGAAAAATAAAGTGGA
AAATAAACGGATTGCAGTTCATCATAACTCAGTTGAGGACGTTCCTGAAGAGAAAGAGAATGAAATCAAACCAGACCGTGAAGAAATTGTAGAAAATCCA
AGTTCTTCAGCTTCTGAATCTAATACAAGTACTTCCATTGTAAACAGAATACATCCAAGTACTGCCAGCACGGTAGTGACACCAGCAGCAGTTCTTCCTG
GATTGGCACAGGTGATAACTGCTGTATCTGCTCAGCAGAATTCTAATTTGATTCCCAAAGTCTTAATCCCTGTTAATAGCATTCCCACCTACAATGCTGC
ATTGGATAACAATCCCCTTTTACTTAACACCTACAACAAGTTCCCTTACCCAACAATGTCAGAAATTACAGTTCTTTCTGCTCAAGCAAAATATACAGAG
GAACAGATCAAGATATGGTTTTCAGCCCAACGTTTAAAACATGGTGTTAGTTGGACTCCCGAGGAAGTAGAGGAGGCAAGAAGGAAACAATTCAATGGAA
CAGTGCATACTGTACCTCAGACCATAACTGTTATTCCTACACACATTTCCACAGGGAGTAATGGTTTACCATCTATTTTACAGACATGCCAAATAGTTGG
TCAGCCTGGTCTGGTCCTTACTCAAGTGGCTGGAACAAACACCTTGCCAGTTACAGCACCTATAGCCTTGACAGTGGCAGGCGTTCCAAGTCAAAATAAT
ATACAGAAAAGTCAGGTACCTGCTGCTCAGCCTACTGCAGAAACAAAGCCAGCAACAGCAGCAGTTCCAACTTCTCAAAGTGTCAAACATGAAACTGCAT
TGGTAAACCCTGATTCATTTGGCATTCGGGCAAAAAAGACAAAAGAGCAACTGGCAGAATTAAAAGTTAGCTACCTTAAAAATCAGTTTCCCCATGATTC
AGAAATTATCAGACTTATGAAAATAACAGGCCTGACGAAAGGAGAGATTAAAAAATGGTTTAGTGACACAAGGTACAACCAGAGAAATTCAAAGAGTAAT
CAGTGCTTACATCTCAACAATGATTCCTCTACCACCATTATTATAGACTCCAGTGATGAAACCACGGAATCCCCAACTGTTGGTACTGCACAGCCTAAGC
AATCCTGGAATCCTTTTCCTGACTTTACTCCCCAAAAGTTTAAAGAGAAAACTGCAGAGCAGCTTCGTGTCCTTCAGGCAAGTTTTCTCAACAGCTCTGT
ACTTACAGATGAAGAATTAAATAGGTTAAGGGCACAAACCAAACTTACCAGAAGAGAAATCGATGCTTGGTTTACAGAGAAGAAGAAATCAAAAGCTTTA
AAGGAAGAGAAAATGGAAATAGATGAAAGTAATGCAGGTAGTTCCAAAGAAGAAGCTGGAGAAACTTCTCCTGCAGATGAATCTGGTGCACCTAAGTCAG
GGAGTACAGGCAAGATATGTAAAAAAACACCTGAGCAGCTGCACATGCTTAAGAGTGCATTTGTCCGGACACAGTGGCCATCACCAGAAGAGTATGACAA
GTTGGCCAAAGAAAGCGGGCTTGCTAGAACAGACATAGTTAGTTGGTTTGGGGACACCCGTTATGCTTGGAAGAATGGAAACTTGAAATGGTACTACTAC
TATCAGAGCGCCAATTCAAGTAGTATGAATGGTCTGTCTTCCCTTAGGAAAAGAGGGAGAGGGAGACCCAAAGGACGGGGAAGAGGAAGACCGCGTGGGC
GGCCTAGAGGAAGCAAAAGAATTAACAACTGGGACAGGGGACCATCACTCATAAAATTTAAAACTGGAACTGCAATACTTAAGGATTATTACCTGAAGCA
CAAGTTTCTTAATGAGCAAGACCTTGATGAACTTGTTAACAAATCACATATGGGCTATGAGCAGGTCAGAGAGTGGTTTGCAGAAAGACAGAGAAGATCA
GAATTAGGTATAGAATTATTTGAGGAAAATGAGGAGGAAGATGAAGTTATTGATGACCAGGAAGAGGATGAAGAAGAAACAGATGATAGTGACACTTGGG
AACCTCCACGACATGTGAAACGGAAGCTGTCTAAATCAGATGACTGAAATCTGCCTAAAACGTTGAAGGAGAATCAATTCTTCAACTCAAGATGTCTGAT
TTACTGTGAATTTGCCCAATCTTTGATGACATTGAAAACGTTTTGGGGCATACACACTCAAAAAGCAGGATCCAATACCCAAAAGAAATGGAACTTAATG
TTGTGCCAAAGTTAAACTACTGCAGTTGGTGGAAGTTCTGCAATGTAAATAGAACACTAATTAAAAAACAACTTGTAAAAATGCAATTTAAATTTTAATA
CAGTACATTTTTCTTCTAATATGATGGAGACATTCTGAATCTTAGACTTTCTGAGGGGGTTTAATGACCACTAGAGCTTGTCCTCATATTCAGTCCAGTT
TAATACTGTATGTCTAGTAAGATGGGCTATATATTGCCTCTATTCTTTGAGATGTGATTAAGCTTAGAACTTTGACCAAAAATTTTACAGTAAAAATTGT
TAGAAATGGGTAAAAAAAAAAAAAAAAAAAAAACCCTGATTTATTACTATGTCTTATTTATCAGACCCTGCACTAAGATTAAAAGTTGTGCATGGGCTTA
TACAATTTCAAATGTAAAAACAGCCTATTTATATATGTTGAAACAATAAGATTTTATGGTTTCACTGGCCCTTAATAATTGGATCCTTCATTAAACACAT
CCATGAATACCACTGTTTCTACTGTTACTTGTTCCATTAGAAGCTGATTTGCTTTTTATTAAACTGCAGGAAATTAATTCTGAAGAAGGAAAAGAAACTG
ACATGGTAATTTGGATAGGGTGAAGCTTTGAAATAAATACTGCACCAAAAATGTGAAAAAGAATCACGTAGTAAATGTATACTTAGTGTTTTCTTACAAT
CGTGGAAATCAGAAATGTTTATACAGGTGTCAGATCAGAATTTTTAAGAGTTATCTTTCAGTGAAATTTTATAACAGTTTCAGTGAATAGAGAAAAAATT
CTGAACTGATCATAATGAGTTAAAAGTTAGCTTTTCACTTATGAATCATGGGAGTGTTAACTGTTAACTCTTGCCGTATCAGGAGACTGAAAGTTCATAC
TAATTTACAAACTTTGTAAAGACTGGTGCTGTTCATTCATATTGGGAAGAACCCTTTCTTTTGTGACCATAGGACCATTTTTCAAAATGCGCTTTCTTCG
TAGAAGAAATGTTCAGTTTAATTAGCTTTGGATTTTAACATGTTTTTCTGAATGACCAGATGTAGGCTTAGCCAGTTTATTTTCTCTAATTTAATGGATA
ATAAATATTAAGCTCTTGGTTAAATGTTTTTCAGCCATTGAAAATGTTATGAAAAAGCCTTGAATATGCATGCTGCTTTCATTTTTATAAACTTTTTATT
TTTTAACTATAGCTTTATTAGTAATTCCAAAATGCTGCAATGTAGCTGATTTCCTCACTCAGACAGCTGGCTTTGTGGGTGGCTTTTTTCATACTACTGT
TAACTTTTTTAAAAAAGAAGCAATGTAAAGACCGTAATGCACTAAGCTTTTTTTTTTTCTTTTACACGTTTAAAAACTTCTTAAAGCACTCTGTAATATA
ATGATTAAATTGCTCCCTTTTATAAACATTGCTAATGTGGTCTGAGTTTTGTTTGACAATAATTTTAATGTTCTCAAAATTTGAAATGTCAAGTATTAGA
AATGAAGCCTTTGAATATAGTTGTTTCCCTTGCAATATGTTAACTCTGTGTAGCTGTATATAACTATTAATTTCCCTCATCTCTTTTATAGGCCTGATAA
AACTCTTCTCTGACGTTTGCTAGCTTTTAAATATATTTATTTGACAGAGCAAAAGAACCCTCTTCTGGTTGATTGTGACCTTGAATACAAAATAAAAGTA
GTGATTAGTAGTTTA
  • refseq_ZHX1.F1 (forward primer)
  • refseq_ZHX1.R1 (reverse primer)
  • Alternative donor

Frame 0

TGQS*LPEKCSRFEMQDGGGVALRGAAAPAGSG
ASRLRDQRARRLWAAGGNWSAESAAGTGK
EKTDC
CFGPVGNDGLS*TSN*KGYPSISKNRMLRKLWQ
VIGVVLQKFQKFSSILSANNKLFT*LHHEKAAN
ETC*AQKWT*RTKSHCFQMKNTEQF*ASMLFNHH
*AFPHNIRMASRRKSTTPCMVLASEQDPDLELI
SDLDEGPPVLTPVENTRAESISSDEEVHESVDS
DNQQNKKVEGGYECKYCTFQTPDLNMFTFHVDSE
HPNVVLNSSYVCVECNFLTKRYDALSEHNLKYH
PGEENFKLTMVKRNNQTIFEQTINDLTFDGSFV
KEENAEQAESTEVSSSGISISKTPIMKMMKNKVE
NKRIAVHHNSVEDVPEEKENEIKPDREEIVENP
SSSASESNTSTSIVNRIHPSTASTVVTPAAVLP
GLAQVITAVSAQQNSNLIPKVLIPVNSIPTYNAA
LDNNPLLLNTYNKFPYPTMSEITVLSAQAKYTE
EQIKIWFSAQRLKHGVSWTPEEVEEARRKQFNG
TVHTVPQTITVIPTHISTGSNGLPSILQTCQIVG
QPGLVLTQVAGTNTLPVTAPIALTVAGVPSQNN
IQKSQVPAAQPTAETKPATAAVPTSQSVKHETA
LVNPDSFGIRAKKTKEQLAELKVSYLKNQFPHDS
EIIRLMKITGLTKGEIKKWFSDTRYNQRNSKSN
QCLHLNNDSSTTIIIDSSDETTESPTVGTAQPK
QSWNPFPDFTPQKFKEKTAEQLRVLQASFLNSSV
LTDEELNRLRAQTKLTRREIDAWFTEKKKSKAL
KEEKMEIDESNAGSSKEEAGETSPADESGAPKS
GSTGKICKKTPEQLHMLKSAFVRTQWPSPEEYDK
LAKESGLARTDIVSWFGDTRYAWKNGNLKWYYY
YQSANSSSMNGLSSLRKRGRGRPKGRGRGRPRG
RPRGSKRINNWDRGPSLIKFKTGTAILKDYYLKH
KFLNEQDLDELVNKSHMGYEQVREWFAERQRRS
ELGIELFEENEEEDEVIDDQEEDEEETDDSDTW
EPPRHVKRKLSKSDD*NLPKTLKENQFFNSRCLI
YCEFAQSLMTLKTFWGIHTQKAGSNTQKKWNLM
LCQS*TTAVGGSSAM*IEH*LKNNL*KCNLNFN
TVHFSSNMMETF*ILDFLRGFNDH*SLSSYSVQF
NTVCLVRWAIYCLYSLRCD*A*NFDQKFYSKNC
*KWVKKKKKKKP*FITMSYLSDPALRLKVVHGL
IQFQM*KQPIYIC*NNKILWFHWPLIIGSFIKHI
HEYHCFYCYLFH*KLICFLLNCRKLILKKEKKL
TW*FG*GEALK*ILHQKCEKESRSKCILSVFLQ
SWKSEMFIQVSDQNF*ELSFSEIL*QFQ*IEKKF
*TDHNELKVSFSLMNHGSVNC*LLPYQETESSY
*FTNFVKTGAVHSYWEEPFLL*P*DHFSKCAFF
VEEMFSLISFGF*HVFLNDQM*A*PVYFL*FNG*
*ILSSWLNVFQPLKML*KSLEYACCFHFYKLFI
F*L*LY**FQNAAM*LISSLRQLALWVAFFILL
LTFLKKKQCKDRNALSFFFFFYTFKNFLKHSVI*
*LNCSLL*TLLMWSEFCLTIILMFSKFEMSSIR
NEAFEYSCFPCNMLTLCSCI*LLISLISFIGLI
KLFSDVC*LLNIFI*QSKRTLFWLIVTLNTK*K*
*LVV

Frame 1

QVRVDSLKSAAGLKCKMAAAWR*EARRPLQVVA
RAGSATSGPGDSGLRVGTGAQSPRPARER
RRQT
AALDLLVMMA*AKHLTRRDTLPFQRTEC*GSCGK
*LELCFKNFRNSAVFYLPTISSLLDCTMRKLLM
RLVEHKNGLEEPKAIVFK*RTLNSFKPRCFLIT
TELFLITSEWQAGENQQHLAWSLPVNKIQTLS*Y
QIWMKVLLCLHL*KTPEQRVSQVMKRFMNLWIQ
TISKIKKLKVDMNVNIVLFKLQI*ICLLFMWIR
NIPM*C*IHPMFVSNAIFLPKGMMHFLSII*NIT
QEKRILS*LW*NVITRQSLNKQ*MI*LLMVVLL
KRRMQSKQNLQKFLLREYLSVKLLS*K**KIKW
KINGLQFIITQLRTFLKRKRMKSNQTVKKL*KIQ
VLQLLNLIQVLPL*TEYIQVLPAR**HQQQFFL
DWHR**LLYLLSRILI*FPKS*SLLIAFPPTML
HWITIPFYLTPTTSSLTQQCQKLQFFLLKQNIQR
NRSRYGFQPNV*NMVLVGLPRK*RRQEGNNSME
QCILYLRP*LLFLHTFPQGVMVYHLFYRHAK*L
VSLVWSLLKWLEQTPCQLQHL*P*QWQAFQVKII
YRKVRYLLLSLLQKQSQQQQQFQLLKVSNMKLH
W*TLIHLAFGQKRQKSNWQN*KLATLKISFPMI
QKLSDL*K*QA*RKERLKNGLVTQGTTREIQRVI
SAYISTMIPLPPLL*TPVMKPRNPQLLVLHSLS
NPGILFLTLLPKSLKRKLQSSFVSFRQVFSTAL
YLQMKN*IG*GHKPNLPEEKSMLGLQRRRNQKL*
RKRKWK*MKVMQVVPKKKLEKLLLQMNLVHLSQ
GVQARYVKKHLSSCTCLRVHLSGHSGHHQKSMT
SWPKKAGLLEQT*LVGLGTPVMLGRMET*NGTTT
IRAPIQVV*MVCLPLGKEGEGDPKDGEEEDRVG
GLEEAKELTTGTGDHHS*NLKLELQYLRIIT*S
TSFLMSKTLMNLLTNHIWAMSRSESGLQKDREDQ
N*V*NYLRKMRRKMKLLMTRKRMKKKQMIVTLG
NLHDM*NGSCLNQMTEICLKR*RRINSSTQDV*
FTVNLPNL**H*KRFGAYTLKKQDPIPKRNGT*C
CAKVKLLQLVEVLQCK*NTN*KTTCKNAI*ILI
QYIFLLI*WRHSES*TF*GGLMTTRACPHIQSS
LILYV**DGLYIASIL*DVIKLRTLTKNFTVKIV
RNG*KKKKKKNPDLLLCLIYQTLH*D*KLCMGL
YNFKCKNSLFIYVETIRFYGFTGP**LDPSLNT
SMNTTVSTVTCSIRS*FAFY*TAGN*F*RRKRN*
HGNLDRVKL*NKYCTKNVKKNHVVNVYLVFSYN
RGNQKCLYRCQIRIFKSYLSVKFYNSFSE*RKN
SELIIMS*KLAFHL*IMGVLTVNSCRIRRLKVHT
NLQTL*RLVLFIHIGKNPFFCDHRTIFQNALSS
*KKCSV*LALDFNMFF*MTRCRLSQFIFSNLMD
NKY*ALG*MFFSH*KCYEKALNMHAAFIFINFLF
FNYSFISNSKMLQCS*FPHSDSWLCGWLFSYYC
*LF*KRSNVKTVMH*AFFFSFTRLKTS*STL*Y
ND*IAPFYKHC*CGLSFV*Q*F*CSQNLKCQVLE
MKPLNIVVSLAIC*LCVAVYNY*FPSSLL*A**
NSSLTFASF*IYLFDRAKEPSSG*L*P*IQNKS
SD**F

Frame 2

RSELTP*KVQPV*NARWRRRGAERRGGPCR*WR
EPAPRPAGPETLGCGWELERRVRGRHGK
GEDRL
LLWTCW**WPELNI*LEGIPFHFKEQNAKEAVA
SDWSCASKISEIQQYFICQQ*ALYLIAP*ESC**
DLLSTKMDLKNQKPLFSNEEH*TVLSLDAF*SP
LSFSS*HQNGKQAKINNTLHGPCQ*TRSRP*VD
IRFG*RSSCAYTCRKHQSREYLK**RGS*ICGFR
QSAK*KS*RWI*M*ILYFSNSRSKYVYFSCGFG
TSQCSAKFILCLCRMQFSYQKV*CTF*A*SEIS
PRRREF*VDYGET**PDNL*TNNK*SDF*W*FC*
RGECRASRIYRSFFFGNIYQ*NSYHENDEK*SG
K*TDCSSS*LS*GRS*RERE*NQTRP*RNCRKS
KFFSF*I*YKYFHCKQNTSKYCQHGSDTSSSSSW
IGTGDNCCICSAEF*FDSQSLNPC**HSHLQCC
IG*QSPFT*HLQQVPLPNNVRNYSSFCSSKIYR
GTDQDMVFSPTFKTWC*LDSRGSRGGKKETIQWN
SAYCTSDHNCYSYTHFHRE*WFTIYFTDMPNSW
SAWSGPYSSGWNKHLASYSTYSLDSGRRSKSK*
YTEKSGTCCSAYCRNKASNSSSSNFSKCQT*NCI
GKP*FIWHSGKKDKRATGRIKS*LP*KSVSP*F
RNYQTYENNRPDERRD*KMV**HKVQPEKFKE*
SVLTSQQ*FLYHHYYRLQ**NHGIPNCWYCTA*A
ILESFS*LYSPKV*RENCRAASCPSGKFSQQLC
TYR*RIK*VKGTNQTYQKRNRCLVYREEEIKSF
KGRENGNR*K*CR*FQRRSWRNFSCR*IWCT*VR
EYRQDM*KNT*AAAHA*ECICPDTVAITRRV*Q
VGQRKRAC*NRHS*LVWGHPLCLEEWKLEMVLL
LSERQFK*YEWSVFP*EKRERETQRTGKRKTAWA
A*RKQKN*QLGQGTITHKI*NWNCNT*GLLPEA
QVS**ARP**TC*QITYGL*AGQRVVCRKTEKI
RIRYRII*GK*GGR*SY**PGRG*RRNR***HLG
TSTTCETEAV*IR*LKSA*NVEGESILQLKMSD
LL*ICPIFDDIENVLGHTHSKSRIQYPKEMELN
VVPKLNYCSWWKFCNVNRTLIKKQLVKMQFKF*Y
STFFF*YDGDILNLRLSEGV**PLELVLIFSPV
*YCMSSKMGYILPLFFEM*LSLEL*PKILQ*KL
LEMGKKKKKKKTLIYYYVLFIRPCTKIKSCAWAY
TISNVKTAYLYMLKQ*DFMVSLALNNWILH*TH
P*IPLFLLLLVPLEADLLFIKLQEINSEEGKET
DMVIWIG*SFEINTAPKM*KRIT**MYT*CFLTI
VEIRNVYTGVRSEFLRVIFQ*NFITVSVNREKI
LN*S**VKS*LFTYESWEC*LLTLAVSGD*KFI
LIYKLCKDWCCSFILGRTLSFVTIGPFFKMRFLR
RRNVQFN*LWILTCFSE*PDVGLASLFSLI*WI
INIKLLVKCFSAIENVMKKP*ICMLLSFL*TFY
FLTIALLVIPKCCNVADFLTQTAGFVGGFFHTTV
NFFKKEAM*RP*CTKLFFFLLHV*KLLKALCNI
MIKLLPFINIANVV*VLFDNNFNVLKI*NVKY*
K*SL*I*LFPLQYVNSV*LYITINFPHLFYRPDK
TLL*RLLAFKYIYLTEQKNPLLVDCDLEYKIKV
VISSL

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