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Sequences

Sequence NM_007324

ATGCTGAAGCGGAGGCGGCCGCTGCTCGAGGATCAAACAGAGCATACTGAATCAGCAGGACTGGCTGGTGGTGCAGCAGACATCATGAGTAAGCACCGAG
AAGTCTGTTCCTTATCACGTGTGTAAGGGGAAAAAGGTTTAAACAAGTCTCTTAAGTGGTGTTTCCTCACCGATGGAGAATTACTTCCAAGCAGAAGCTT
ACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCC
TTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCAT
TCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTG
TTGCAGAAGACCAGTTAATTAAGAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCTTGTCTGCC
AGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCC
CTTATGGATGCTTTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAG
AGAAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTCTGTTCCCC
TTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGAT
GGATGTCCTGCTGTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATCTAGGGAGTCCAAATTCCT
TTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACC
AGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAA
CATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACTAATTGGAAGTTGACTAAACTAAATGAGA
TGAATGATAGCCAAGTAAACGAAGAAAAGGAAAAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGC
AGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAAT
GGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGG
AAAGCAAGTCAGAATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGACCTAATGAA
GAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAATCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTT
GGTGGTGCAAGACCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAGTGGAAATA
ATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGA
TACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGT
CAGTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCTCAGGCTCCAAATTGCATGAAATGTGAAG
CCAGG
TTTACATTCACCAAAAGGAGGCATCACTGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGA
CAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGTGGCTCAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTG
CCCAAT
GGAGAAGTTGCTGATGCAGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCACACGACCCAGTCAAGCCAGTAACTA
CCAGTCCTCTACCAGCAGAGACGGATATTTGTCTATTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCTGAAGA
TGGCCTTCCTCCCATTCTCATCTCCACTGGTGTAAAAGGAGACTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGT
GGCCCTGACCCACTTGTATTTGTTTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGAAGTGCTGGTGTTTCACAACCAAGG
GAATGCATGCAGTGGGTCAGTCTGAGATAGTCATTCTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTGCAGCT
TTATCGGGATGCTCTGGCAGGGAATGTGGTGAGCAACTTGGGACATTCCTTCTTCAGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATAT
GTGACATCTACCTACCAGTCACTGCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAGAAATGGGAAACTCCTTGGGCTAAAG
TATTTCCTATCCGTCTGATGTTGAGACTTGGAGCTGAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGAGAGAC
GGGGCATACCATCATGAATCTTCTTGCAGACTTCAGAAATTACCAGTATACCTTGCCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACT
AGCATCAAAATTCCCAGCAACAGATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGG
CAGACTCTCATCTTGTGTGTGTACAGAATGATGATGGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCCAGTTT
CTTTGTGTTCAGTGGCGCTCTGAAATCCTCTTCTGGATACCTTGCCAAGTCCAGTATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATG
GATTCCTTGAGGCAGGCACTGCGAGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCCCAGGAGCACATCCACATCCAGTGGG
TGGATGATGACAAGAACGTTAGCAAGGGTGTCGTAAGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCAGAATA
TAAAGCAAATGGAAAAGTAATCAGATGGACAGAGGTGTTTTTCCTAGAAAACGATGACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTG
ACTGAGCATGTTGCCAAAGCTTTTTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACCAAACTGGGACTACGTGTGACACTTG
ACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATCCATGG
AGGGGCCTGCCAGCTTAGTGAGGGCCCCGTTGTCATGGAACTCATCTTTTATATTCTGGAAAACATCGTATAAACAGAGAAGACTTCATTTTTTTCTGTT
CAGACTTGTTGCAACAGCAGTCATACCCAAATCATTTGCACTTTAAAACTGGAAGATTAAGCTTTTGTTAACACTATTAATGGGGTGGGGAATAGGGTGG
GAGTGGGGGTTTGGGAGACGGGTGGGAAAGGGTGGTTGGGGGGACCGATGTTCCATAATTCTAAGTCTTCTATGCATTGTCCACCAAGAAGATCTGGGCA
GCTTCTGTTCCTGCACAACAGTTATGCTATCCTTGCAGCTAATCCCCTTCTGTTACTGTTTAGACAAGAATTCCGCTCCTCTCTCAAGATTTACTTATGG
TCATGTGCTCAGAAATGCTCAAATGGGTACAACCATCACCAAGGGTGGGATGGGAGGGCAGAGGGGAAATAAAATATAAAGCATCAGTTCTTGCACTCTT
TGTACAGAATTGGTATAAAAAGGATAATTCCACACTGA
  • refseq_ZFYVE9.F2 (forward primer)
  • refseq_ZFYVE9.R2 (reverse primer)
  • Single exon skipping

Frame 0

MLKRRRPLLEDQTEHTESAGLAGGAADIMSKHR
EVCSLSRV*GEKGLNKSLKWCFLTDGELLPSRSL
QPGQGVR*I*TKRR*NSFFYFIGYKVE*DSRSP
FSPAVI*PYFGQCE*ICSF**VTTTTESLLPGS
FSSPDHRGRGSLC*WTGL*SKSRDCHNVD**KCC
CRRPVN*EKL*LG*SMQCC*SGREEMWKPGLSA
R*EECSCCSRHA*L**KDITKRFTGL**L**SI
PYGCF*LFTG**KQTN*SI*F*YK*VH*KRYEFR
ETNGSIE*TENRGEIC*PSVSYFI**SSQCLFP
FTIKG*RKYR*RPLHVCDYKFNG*FSNLIPGNR
WMSCC*KARELYTR*GPHWQNQLS*DRSRESKFL
FPHE*GDFDEKRASRGEHH*RIPPVWFTFASQT
RHA*WVWKE**L*TVFRLPCA**S*G**K*RL*
T*RNSWHYRIP*YDRAFL*ISGHD*LEVD*TK*D
E**PSKRRKGKVSTD*SA*GH*W**WRTVCWIG
RCRSRFKRNLH**K*RM*FLHCYRHTSSKLSI*
WL*FLWNARPRCFFCSKDFTLQRRFSNRRKRNRG
KQVRMLLKYL*TERK*GHRREWTTFKQHW*PNE
EKLFT*FL*SSSISAWAIFPQGSSKPAIYQCSF
WWCKTQATF*S*TSNSKAIIRPFTK*LSCKQWK*
Y*K*K*YSWESKIRGKLSNQCMQSIFGKHL*CR
YKWGTFRKL*G*DLH*TMPCISSR*PR**SQSW
SVWNFCQKAIHHSG*GGSSMGTGFSGSKLHEM*S
Q
VYIHQKEASLQSMWEGFLCFLL*PEM*TVIHG
QKGS*SVCNLPFSANECGSAQRAEASLVC*WDL
AQ
WRSC*CSQINNEWNFLCRNPGCVTRPSQASNY
QSSTSRDGYLSILWEYNSGWKSCWKCNESYS*R
WPSSHSHLHWCKRRLCCGRETITDFSNAAVGGW
WP*PTCICFKCKFVVNG*NCKLCEQEVLVFHNQG
NACSGSV*DSHSSTVFTG*KVFAKGYL*SLCAA
LSGCSGRECGEQLGTFLLQSKFPWQ*RTWWILI
CDIYLPVTARPSTPNPTLLVWDSYPEMGNSLG*S
ISYPSDVETWS*ISTLSMPTIQCQISEAIVWRD
GAYHHESSCRLQKLPVYLASSSRFGG*YGSSEN
*HQNSQQQIQ*DDESHEQVQ*ACPGRRCLLQ*KG
RLSSCVCTE**WKLSDPGYQYSQSAQKSDWCQF
LCVQWRSEILFWIPCQVQYCGRWCYGPDYCREH
GFLEAGTARDEGLHHHLWEGGRGGTPGAHPHPVG
G**QER*QGCRKSYRWEVHGDYNKCEDIPWIRI
*SKWKSNQMDRGVFPRKR*PAQLPQ*SCRSQ*I
D*ACCQSFLPCSLSSPETSEGRWNDQTGTTCDT*
LRSGWLSSREQWPAPSLAVHE*SGQRLGAGDPW
RGLPA**GPRCHGTHLLYSGKHRINREDFIFFC
SDLLQQQSYPNHLHFKTGRLSFC*HY*WGGE*GG
SGGLGDGWERVVGGTDVP*F*VFYALSTKKIWA
ASVPAQQLCYPCS*SPSVTV*TRIPLLSQDLLM
VMCSEMLKWVQPSPRVGWEGRGEIKYKASVLALF
VQNWYKKDNSTL

Frame 1

C*SGGGRCSRIKQSILNQQDWLVVQQTS*VSTE
KSVPYHVCKGKKV*TSLLSGVSSPMENYFQAEA
YNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPP
SHRLSFNPTLASVNESAVSNESQPQLKVFSLAH
SAPLTTEEEDHCANGQDCNLNPEIATMWIDENA
VAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLP
DEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQS
LMDAFSCSLDNENRQTDQFSFSINESTEKDMNS
EKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSP
SQLKDDGSIGRDPSMSAITSLTVDSVISSQGTD
GCPAVKKQENYIPDEDLTGKISSPRTDLGSPNS
FSHMSEGILMKKEPAEESTTEESLRSGLPLLLKP
DMPNGSGRNNDCERCSDCLVPNEVRADENEGYE
HEETLGTTEFLNMTEHFSESQDMTNWKLTKLNE
MNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLA
DAGLDLKGTCISESEECDFSTVIDTPAANYLSN
GCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIE
ESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMK
KNYLHNFCSQVPSVLGQSSPKVVASLPSISVPF
GGARPKQPSNLKLQIPKPLSDHLQNDFPANSGN
NTKNKNDILGKAKLGENSATNVCSPSLGNISNVD
TNGEHLESYEAEISTRPCLALAPDSPDNDLRAG
QFGISARKPFTTLGEVAPVWVPDSQAPNCMKCE
AR
FTFTKRRHHCRACGKVFCASCCSLKCKLLYMD
RKEARVCVICHSVLMNVAQPREQRRVWFADGIL
PN
GEVADAAKLTMNGTSSAGTLAVSHDPVKPVT
TSPLPAETDICLFSGSITQVGSPVGSAMNLIPED
GLPPILISTGVKGDYAVEEKPSQISVMQQLEDG
GPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTK
GMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQL
YRDALAGNVVSNLGHSFFSQSFLGSKEHGGFLY
VTSTYQSLQDLVLPTPPYLFGILIQKWETPWAK
VFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGET
GHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKT
SIKIPSNRYNEMMKAMNKSNEHVLAGGACFNEK
ADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASF
FVFSGALKSSSGYLAKSSIVEDGVMVQITAENM
DSLRQALREMKDFTITCGKADAEEPQEHIHIQW
VDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEY
KANGKVIRWTEVFFLENDDQHNCLSDPADHSRL
TEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTL
DSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHG
GACQLSEGPVVMELIFYILENIV*TEKTSFFSV
QTCCNSSHTQIICTLKLED*AFVNTINGVGNRV
GVGVWETGGKGWLGGPMFHNSKSSMHCPPRRSGQ
LLFLHNSYAILAANPLLLLFRQEFRSSLKIYLW
SCAQKCSNGYNHHQGWDGRAEGK*NIKHQFLHS
LYRIGIKRIIPH*

Frame 2

AEAEAAAARGSNRAY*ISRTGWWCSRHHE*APR
SLFLITCVRGKRFKQVS*VVFPHRWRITSKQKL
TTWTRC*MNLNKTKMKQFLLLYWIQSGIRF*IP
LLTGCHLTLLWPV*MNLQFLMSHNHN*KSSPWLI
QLP*PQRKRITVLMDRTVI*IQRLPQCGLMKML
LQKTS*LRETIVGMINAVLLKWERRNVETWLVC
QMRRMFLL*PSCITVIKGHYKTIYRIVIIIIVNP
LWMLLAVHWIMKTDKLINLVLV*MSPLKKI*IQ
RNKWIH*IDRKQRGDLLTICVLLHLIV*PVSVP
LHN*RMTEV*VETPPCLRLQV*RLIQ*SHPREQM
DVLLLKSKRTIYQMRTSLAKSALLGQI*GVQIP
FPT*VRGF**KKSQQRRAPLKNPSGLVYLCFSN
QTCLMGLEGIMTVNGVQIALCLMKLGLMKMKVMN
MKKLLALQNSLI*QSISLNLRT*LIGS*LN*MR
*MIAK*TKKRKSFYRLVSLRTLMVIVEDSVLDW
QMQV*I*KELALVKVKNVISPLL*THQQQIIYLM
VVIPMECKTQVFLLFQRLYPPKKIQ*QKKKK*R
KASQNATQIFMNREEMRPQKGVDYF*TALVT**
RKIIYIISVVKFHQCLGNLPPR**QACHLSVFLL
VVQDPSNLLILNFKFQSHYQTIYKMTFLQTVEI
ILKIKMIFLGKQN*GKTQQPMYAVHLWETSLMS
IQMGNI*KVMRLRSPLDHALH*LQIAQIMISELV
SLEFLPESHSPLWVRWLQYGYRILRLQIA*NVK
PG
LHSPKGGITAEHVGRFSVLPAVA*NVNCYTW
TERKLECV*SAIQC**MWLSPESRGEFGLLMGSC
PM
EKLLMQPN*Q*MELPLQEPWLCHTTQSSQ*L
PVLYQQRRIFVYSLGV*LRLEVLLEVQ*ILFLK
MAFLPFSSPLV*KETMLWKRNHHRFQ*CSSWRMV
ALTHLYLF*MQICCQWLKL*IM*TGSAGVSQPR
ECMQWVSLR*SFFYSVYRMKSVCQRISLITLCS
FIGMLWQGMW*ATWDIPSSVKVSLAVKNMVDSYM
*HLPTSHCKT*YSQPHLTCLGFLSRNGKLLGLK
YFLSV*C*DLELNIDFIHAHYSVSDFGSHCLER
RGIPS*IFLQTSEITSIPCQ*FKVWWLIWKFGKL
ASKFPATDTMR**KP*TSPMSMSWQEVPASMKR
QTLILCVYRMMMETIRPRLSVFTISPEK*LVPV
SLCSVAL*NPLLDTLPSPVLWKMVLWSRLLQRTW
IP*GRHCER*RTSPSPVGRRTRRNPRSTSTSSG
WMMTRTLARVS*VL*MGSPWRL*QM*RYSMDQN
IKQMEK*SDGQRCFS*KTMTSTIASVILQITVD*
LSMLPKLFALLSVLT*NF*RKME*PNWDYV*HL
TQIRLAIKQGAMASPFPRST*MIWTAPWCR*SM
EGPASLVRAPLSWNSSFIFWKTSYKQRRLHFFLF
RLVATAVIPKSFAL*NWKIKLLLTLLMGWGIGW
EWGFGRRVGKGGWGDRCSIILSLLCIVHQEDLG
SFCSCTTVMLSLQLIPFCYCLDKNSAPLSRFTYG
HVLRNAQMGTTITKGGMGGQRGNKI*SISSCTL
CTELV*KG*FHT

Sequence NM_004799

ATGCTGAAGCGGAGGCGGCCGCTGCTCGAGGATCAAACAGAGCATACTGAATCAGCAGGACTGGCTGGTGGTGCAGCAGACATCATGAGTAAGCACCGAG
AAGTCTGTTCCTTATCACGTGTGTAAGGGGAAAAAGGTTTAAACAAGTCTCTTAAGTGGTGTTTCCTCACCGATGGAGAATTACTTCCAAGCAGAAGCTT
ACAACCTGGACAAGGTGTTAGATGAATTTGAACAAAACGAAGATGAAACAGTTTCTTCTACTTTATTGGATACAAAGTGGAATAAGATTCTAGATCCCCC
TTCTCACCGGCTGTCATTTAACCCTACTTTGGCCAGTGTGAATGAATCTGCAGTTTCTAATGAGTCACAACCACAACTGAAAGTCTTCTCCCTGGCTCAT
TCAGCTCCCCTGACCACAGAGGAAGAGGATCACTGTGCTAATGGACAGGACTGTAATCTAAATCCAGAGATTGCCACAATGTGGATTGATGAAAATGCTG
TTGCAGAAGACCAGTTAATTAAGAGAAACTATAGTTGGGATGATCAATGCAGTGCTGTTGAAGTGGGAGAGAAGAAATGTGGAAACCTGGCTTGTCTGCC
AGATGAGAAGAATGTTCTTGTTGTAGCCGTCATGCATAACTGTGATAAAAGGACATTACAAAACGATTTACAGGATTGTAATAATTATAATAGTCAATCC
CTTATGGATGCTTTTAGCTGTTCACTGGATAATGAAAACAGACAAACTGATCAATTTAGTTTTAGTATAAATGAGTCCACTGAAAAAGATATGAATTCAG
AGAAACAAATGGATCCATTGAATAGACCGAAAACAGAGGGGAGATCTGTTAACCATCTGTGTCCTACTTCATCTGATAGTCTAGCCAGTGTCTGTTCCCC
TTCACAATTAAAGGATGACGGAAGTATAGGTAGAGACCCCTCCATGTCTGCGATTACAAGTTTAACGGTTGATTCAGTAATCTCATCCCAGGGAACAGAT
GGATGTCCTGCTGTTAAAAAGCAAGAGAACTATATACCAGATGAGGACCTCACTGGCAAAATCAGCTCTCCTAGGACAGATCTAGGGAGTCCAAATTCCT
TTTCCCACATGAGTGAGGGGATTTTGATGAAAAAAGAGCCAGCAGAGGAGAGCACCACTGAAGAATCCCTCCGGTCTGGTTTACCTTTGCTTCTCAAACC
AGACATGCCTAATGGGTCTGGAAGGAATAATGACTGTGAACGGTGTTCAGATTGCCTTGTGCCTAATGAAGTTAGGGCTGATGAAAATGAAGGTTATGAA
CATGAAGAAACTCTTGGCACTACAGAATTCCTTAATATGACAGAGCATTTCTCTGAATCTCAGGACATGACTAATTGGAAGTTGACTAAACTAAATGAGA
TGAATGATAGCCAAGTAAACGAAGAAAAGGAAAAGTTTCTACAGATTAGTCAGCCTGAGGACACTAATGGTGATAGTGGAGGACAGTGTGTTGGATTGGC
AGATGCAGGTCTAGATTTAAAAGGAACTTGCATTAGTGAAAGTGAAGAATGTGATTTCTCCACTGTTATAGACACACCAGCAGCAAATTATCTATCTAAT
GGTTGTGATTCCTATGGAATGCAAGACCCAGGTGTTTCTTTTGTTCCAAAGACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAGG
AAAGCAAGTCAGAATGCTACTCAAATATTTATGAACAGAGAGGAAATGAGGCCACAGAAGGGAGTGGACTACTTTTAAACAGCACTGGTGACCTAATGAA
GAAAAATTATTTACATAATTTCTGTAGTCAAGTTCCATCAGTGCTTGGGCAATCTTCCCCCAAGGTAGTAGCAAGCCTGCCATCTATCAGTGTTCCTTTT
GGTGGTGCAAGACCCAAGCAACCTTCTAATCTTAAACTTCAAATTCCAAAGCCATTATCAGACCATTTACAAAATGACTTTCCTGCAAACAGTGGAAATA
ATACTAAAAATAAAAATGATATTCTTGGGAAAGCAAAATTAGGGGAAAACTCAGCAACCAATGTATGCAGTCCATCTTTGGGAAACATCTCTAATGTCGA
TACAAATGGGGAACATTTAGAAAGTTATGAGGCTGAGATCTCCACTAGACCATGCCTTGCATTAGCTCCAGATAGCCCAGATAATGATCTCAGAGCTGGT
CAGTTTGGAATTTCTGCCAGAAAGCCATTCACCACTCTGGGTGAGGTGGCTCCAGTATGGGTACCGGATTCTCAGGCTCCAAATTGCATGAAATGTGAAG
CCAGG
TTTACATTCACCAAAAGGAGGCATCACTGCAGAGCATGTGGGAAGGTTTTCTGTGCTTCCTGCTGTAGCCTGAAATGTAAACTGTTATACATGGA
CAGAAAGGAAGCTAGAGTGTGTGTAATCTGCCATTCAGTGCTAATGAATGCTCAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCCTAACCCT
AACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCTCTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGG
GAGTTTTAAAGCACCCTGGAGCAGAAG
TGGCTCAGCCCAGAGAGCAGAGGCGAGTTTGGTTTGCTGATGGGATCTTGCCCAATGGAGAAGTTGCTGATGC
AGCCAAATTAACAATGAATGGAACTTCCTCTGCAGGAACCCTGGCTGTGTCACACGACCCAGTCAAGCCAGTAACTACCAGTCCTCTACCAGCAGAGACG
GATATTTGTCTATTCTCTGGGAGTATAACTCAGGTTGGAAGTCCTGTTGGAAGTGCAATGAATCTTATTCCTGAAGATGGCCTTCCTCCCATTCTCATCT
CCACTGGTGTAAAAGGAGACTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTAATGCAGCAGTTGGAGGATGGTGGCCCTGACCCACTTGTATTTGT
TTTAAATGCAAATTTGTTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGAAGTGCTGGTGTTTCACAACCAAGGGAATGCATGCAGTGGGTCAGTCT
GAGATAGTCATTCTTCTACAGTGTTTACCGGATGAAAAGTGTTTGCCAAAGGATATCTTTAATCACTTTGTGCAGCTTTATCGGGATGCTCTGGCAGGGA
ATGTGGTGAGCAACTTGGGACATTCCTTCTTCAGTCAAAGTTTCCTTGGCAGTAAAGAACATGGTGGATTCTTATATGTGACATCTACCTACCAGTCACT
GCAAGACCTAGTACTCCCAACCCCACCTTACTTGTTTGGGATTCTTATCCAGAAATGGGAAACTCCTTGGGCTAAAGTATTTCCTATCCGTCTGATGTTG
AGACTTGGAGCTGAATATCGACTTTATCCATGCCCACTATTCAGTGTCAGATTTCGGAAGCCATTGTTTGGAGAGACGGGGCATACCATCATGAATCTTC
TTGCAGACTTCAGAAATTACCAGTATACCTTGCCAGTAGTTCAAGGTTTGGTGGTTGATATGGAAGTTCGGAAAACTAGCATCAAAATTCCCAGCAACAG
ATACAATGAGATGATGAAAGCCATGAACAAGTCCAATGAGCATGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGGCAGACTCTCATCTTGTGTGTGTA
CAGAATGATGATGGAAACTATCAGACCCAGGCTATCAGTATTCACAATCAGCCCAGAAAAGTGACTGGTGCCAGTTTCTTTGTGTTCAGTGGCGCTCTGA
AATCCTCTTCTGGATACCTTGCCAAGTCCAGTATTGTGGAAGATGGTGTTATGGTCCAGATTACTGCAGAGAACATGGATTCCTTGAGGCAGGCACTGCG
AGAGATGAAGGACTTCACCATCACCTGTGGGAAGGCGGACGCGGAGGAACCCCAGGAGCACATCCACATCCAGTGGGTGGATGATGACAAGAACGTTAGC
AAGGGTGTCGTAAGTCCTATAGATGGGAAGTCCATGGAGACTATAACAAATGTGAAGATATTCCATGGATCAGAATATAAAGCAAATGGAAAAGTAATCA
GATGGACAGAGGTGTTTTTCCTAGAAAACGATGACCAGCACAATTGCCTCAGTGATCCTGCAGATCACAGTAGATTGACTGAGCATGTTGCCAAAGCTTT
TTGCCTTGCTCTCTGTCCTCACCTGAAACTTCTGAAGGAAGATGGAATGACCAAACTGGGACTACGTGTGACACTTGACTCAGATCAGGTTGGCTATCAA
GCAGGGAGCAATGGCCAGCCCCTTCCCTCGCAGTACATGAATGATCTGGACAGCGCCTTGGTGCCGGTGATCCATGGAGGGGCCTGCCAGCTTAGTGAGG
GCCCCGTTGTCATGGAACTCATCTTTTATATTCTGGAAAACATCGTATAAACAGAGAAGACTTCATTTTTTTCTGTTCAGACTTGTTGCAACAGCAGTCA
TACCCAAATCATTTGCACTTTAAAACTGGAAGATTAAGCTTTTGTTAACACTATTAATGGGGTGGGGAATAGGGTGGGAGTGGGGGTTTGGGAGACGGGT
GGGAAAGGGTGGTTGGGGGGACCGATGTTCCATAATTCTAAGTCTTCTATGCATTGTCCACCAAGAAGATCTGGGCAGCTTCTGTTCCTGCACAACAGTT
ATGCTATCCTTGCAGCTAATCCCCTTCTGTTACTGTTTAGACAAGAATTCCGCTCCTCTCTCAAGATTTACTTATGGTCATGTGCTCAGAAATGCTCAAA
TGGGTACAACCATCACCAAGGGTGGGATGGGAGGGCAGAGGGGAAATAAAATATAAAGCATCAGTTCTTGCACTCTTTGTACAGAATTGGTATAAAAAGG
ATAATTCCACACTGA
  • refseq_ZFYVE9.F2 (forward primer)
  • refseq_ZFYVE9.R2 (reverse primer)
  • Single exon skipping

Frame 0

MLKRRRPLLEDQTEHTESAGLAGGAADIMSKHR
EVCSLSRV*GEKGLNKSLKWCFLTDGELLPSRSL
QPGQGVR*I*TKRR*NSFFYFIGYKVE*DSRSP
FSPAVI*PYFGQCE*ICSF**VTTTTESLLPGS
FSSPDHRGRGSLC*WTGL*SKSRDCHNVD**KCC
CRRPVN*EKL*LG*SMQCC*SGREEMWKPGLSA
R*EECSCCSRHA*L**KDITKRFTGL**L**SI
PYGCF*LFTG**KQTN*SI*F*YK*VH*KRYEFR
ETNGSIE*TENRGEIC*PSVSYFI**SSQCLFP
FTIKG*RKYR*RPLHVCDYKFNG*FSNLIPGNR
WMSCC*KARELYTR*GPHWQNQLS*DRSRESKFL
FPHE*GDFDEKRASRGEHH*RIPPVWFTFASQT
RHA*WVWKE**L*TVFRLPCA**S*G**K*RL*
T*RNSWHYRIP*YDRAFL*ISGHD*LEVD*TK*D
E**PSKRRKGKVSTD*SA*GH*W**WRTVCWIG
RCRSRFKRNLH**K*RM*FLHCYRHTSSKLSI*
WL*FLWNARPRCFFCSKDFTLQRRFSNRRKRNRG
KQVRMLLKYL*TERK*GHRREWTTFKQHW*PNE
EKLFT*FL*SSSISAWAIFPQGSSKPAIYQCSF
WWCKTQATF*S*TSNSKAIIRPFTK*LSCKQWK*
Y*K*K*YSWESKIRGKLSNQCMQSIFGKHL*CR
YKWGTFRKL*G*DLH*TMPCISSR*PR**SQSW
SVWNFCQKAIHHSG*GGSSMGTGFSGSKLHEM*S
Q
VYIHQKEASLQSMWEGFLCFLL*PEM*TVIHG
QKGS*SVCNLPFSANECSSLGEHDECLKPEP*P
*QSC*ILFYYPSLAASSGLRSSELSTSHCDGTCG
SFKAPWSRS
GSAQRAEASLVC*WDLAQWRSC*C
SQINNEWNFLCRNPGCVTRPSQASNYQSSTSRD
GYLSILWEYNSGWKSCWKCNESYS*RWPSSHSHL
HWCKRRLCCGRETITDFSNAAVGGWWP*PTCIC
FKCKFVVNG*NCKLCEQEVLVFHNQGNACSGSV
*DSHSSTVFTG*KVFAKGYL*SLCAALSGCSGRE
CGEQLGTFLLQSKFPWQ*RTWWILICDIYLPVT
ARPSTPNPTLLVWDSYPEMGNSLG*SISYPSDV
ETWS*ISTLSMPTIQCQISEAIVWRDGAYHHESS
CRLQKLPVYLASSSRFGG*YGSSEN*HQNSQQQ
IQ*DDESHEQVQ*ACPGRRCLLQ*KGRLSSCVC
TE**WKLSDPGYQYSQSAQKSDWCQFLCVQWRSE
ILFWIPCQVQYCGRWCYGPDYCREHGFLEAGTA
RDEGLHHHLWEGGRGGTPGAHPHPVGG**QER*
QGCRKSYRWEVHGDYNKCEDIPWIRI*SKWKSNQ
MDRGVFPRKR*PAQLPQ*SCRSQ*ID*ACCQSF
LPCSLSSPETSEGRWNDQTGTTCDT*LRSGWLS
SREQWPAPSLAVHE*SGQRLGAGDPWRGLPA**G
PRCHGTHLLYSGKHRINREDFIFFCSDLLQQQS
YPNHLHFKTGRLSFC*HY*WGGE*GGSGGLGDG
WERVVGGTDVP*F*VFYALSTKKIWAASVPAQQL
CYPCS*SPSVTV*TRIPLLSQDLLMVMCSEMLK
WVQPSPRVGWEGRGEIKYKASVLALFVQNWYKK
DNSTL

Frame 1

C*SGGGRCSRIKQSILNQQDWLVVQQTS*VSTE
KSVPYHVCKGKKV*TSLLSGVSSPMENYFQAEA
YNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPP
SHRLSFNPTLASVNESAVSNESQPQLKVFSLAH
SAPLTTEEEDHCANGQDCNLNPEIATMWIDENA
VAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLP
DEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQS
LMDAFSCSLDNENRQTDQFSFSINESTEKDMNS
EKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSP
SQLKDDGSIGRDPSMSAITSLTVDSVISSQGTD
GCPAVKKQENYIPDEDLTGKISSPRTDLGSPNS
FSHMSEGILMKKEPAEESTTEESLRSGLPLLLKP
DMPNGSGRNNDCERCSDCLVPNEVRADENEGYE
HEETLGTTEFLNMTEHFSESQDMTNWKLTKLNE
MNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLA
DAGLDLKGTCISESEECDFSTVIDTPAANYLSN
GCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIE
ESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMK
KNYLHNFCSQVPSVLGQSSPKVVASLPSISVPF
GGARPKQPSNLKLQIPKPLSDHLQNDFPANSGN
NTKNKNDILGKAKLGENSATNVCSPSLGNISNVD
TNGEHLESYEAEISTRPCLALAPDSPDNDLRAG
QFGISARKPFTTLGEVAPVWVPDSQAPNCMKCE
AR
FTFTKRRHHCRACGKVFCASCCSLKCKLLYMD
RKEARVCVICHSVLMNAQAWENMMSASSQSPNP
NNPAEYCSTIPPLQQAQASGALSSPPPTVMVPV
GVLKHPGAE
VAQPREQRRVWFADGILPNGEVADA
AKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAET
DICLFSGSITQVGSPVGSAMNLIPEDGLPPILI
STGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFV
LNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQS
EIVILLQCLPDEKCLPKDIFNHFVQLYRDALAG
NVVSNLGHSFFSQSFLGSKEHGGFLYVTSTYQSL
QDLVLPTPPYLFGILIQKWETPWAKVFPIRLML
RLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNL
LADFRNYQYTLPVVQGLVVDMEVRKTSIKIPSNR
YNEMMKAMNKSNEHVLAGGACFNEKADSHLVCV
QNDDGNYQTQAISIHNQPRKVTGASFFVFSGAL
KSSSGYLAKSSIVEDGVMVQITAENMDSLRQALR
EMKDFTITCGKADAEEPQEHIHIQWVDDDKNVS
KGVVSPIDGKSMETITNVKIFHGSEYKANGKVI
RWTEVFFLENDDQHNCLSDPADHSRLTEHVAKAF
CLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQ
AGSNGQPLPSQYMNDLDSALVPVIHGGACQLSE
GPVVMELIFYILENIV*TEKTSFFSVQTCCNSSH
TQIICTLKLED*AFVNTINGVGNRVGVGVWETG
GKGWLGGPMFHNSKSSMHCPPRRSGQLLFLHNS
YAILAANPLLLLFRQEFRSSLKIYLWSCAQKCSN
GYNHHQGWDGRAEGK*NIKHQFLHSLYRIGIKR
IIPH*

Frame 2

AEAEAAAARGSNRAY*ISRTGWWCSRHHE*APR
SLFLITCVRGKRFKQVS*VVFPHRWRITSKQKL
TTWTRC*MNLNKTKMKQFLLLYWIQSGIRF*IP
LLTGCHLTLLWPV*MNLQFLMSHNHN*KSSPWLI
QLP*PQRKRITVLMDRTVI*IQRLPQCGLMKML
LQKTS*LRETIVGMINAVLLKWERRNVETWLVC
QMRRMFLL*PSCITVIKGHYKTIYRIVIIIIVNP
LWMLLAVHWIMKTDKLINLVLV*MSPLKKI*IQ
RNKWIH*IDRKQRGDLLTICVLLHLIV*PVSVP
LHN*RMTEV*VETPPCLRLQV*RLIQ*SHPREQM
DVLLLKSKRTIYQMRTSLAKSALLGQI*GVQIP
FPT*VRGF**KKSQQRRAPLKNPSGLVYLCFSN
QTCLMGLEGIMTVNGVQIALCLMKLGLMKMKVMN
MKKLLALQNSLI*QSISLNLRT*LIGS*LN*MR
*MIAK*TKKRKSFYRLVSLRTLMVIVEDSVLDW
QMQV*I*KELALVKVKNVISPLL*THQQQIIYLM
VVIPMECKTQVFLLFQRLYPPKKIQ*QKKKK*R
KASQNATQIFMNREEMRPQKGVDYF*TALVT**
RKIIYIISVVKFHQCLGNLPPR**QACHLSVFLL
VVQDPSNLLILNFKFQSHYQTIYKMTFLQTVEI
ILKIKMIFLGKQN*GKTQQPMYAVHLWETSLMS
IQMGNI*KVMRLRSPLDHALH*LQIAQIMISELV
SLEFLPESHSPLWVRWLQYGYRILRLQIA*NVK
PG
LHSPKGGITAEHVGRFSVLPAVA*NVNCYTW
TERKLECV*SAIQC**MLKPGRT**VPQARALTL
TILLNTVLLSLPCSKLRPQEL*ALHLPL*WYLW
EF*STLEQK
WLSPESRGEFGLLMGSCPMEKLLM
QPN*Q*MELPLQEPWLCHTTQSSQ*LPVLYQQRR
IFVYSLGV*LRLEVLLEVQ*ILFLKMAFLPFSS
PLV*KETMLWKRNHHRFQ*CSSWRMVALTHLYL
F*MQICCQWLKL*IM*TGSAGVSQPRECMQWVSL
R*SFFYSVYRMKSVCQRISLITLCSFIGMLWQG
MW*ATWDIPSSVKVSLAVKNMVDSYM*HLPTSH
CKT*YSQPHLTCLGFLSRNGKLLGLKYFLSV*C*
DLELNIDFIHAHYSVSDFGSHCLERRGIPS*IF
LQTSEITSIPCQ*FKVWWLIWKFGKLASKFPAT
DTMR**KP*TSPMSMSWQEVPASMKRQTLILCVY
RMMMETIRPRLSVFTISPEK*LVPVSLCSVAL*
NPLLDTLPSPVLWKMVLWSRLLQRTWIP*GRHC
ER*RTSPSPVGRRTRRNPRSTSTSSGWMMTRTLA
RVS*VL*MGSPWRL*QM*RYSMDQNIKQMEK*S
DGQRCFS*KTMTSTIASVILQITVD*LSMLPKL
FALLSVLT*NF*RKME*PNWDYV*HLTQIRLAIK
QGAMASPFPRST*MIWTAPWCR*SMEGPASLVR
APLSWNSSFIFWKTSYKQRRLHFFLFRLVATAV
IPKSFAL*NWKIKLLLTLLMGWGIGWEWGFGRRV
GKGGWGDRCSIILSLLCIVHQEDLGSFCSCTTV
MLSLQLIPFCYCLDKNSAPLSRFTYGHVLRNAQ
MGTTITKGGMGGQRGNKI*SISSCTLCTELV*KG
*FHT

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