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Sequences

Sequence NM_001119

GCACCCAGGTCGGGCGGTGGGGGCGAGCGGAGGGGCTGAGGGGCGGAGAGGCCTGGCGGGCCGCTGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCC GGAGCCGACGGGCGGTGGCCGCACTGGGACCCCGGAATCCCGCGCGCTGCCCACGATTCGCTTCTGAGGAACCTAGAAAGATTGTACAATGAATGGTGAT TCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGTACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGG AGAGGAACATGGCACCAGACCTTCGCCAGGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAATT GGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGATTGCAGATTTTATGACCACGAATGTACCAAAT GTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTTAAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGT ATGACAAAGGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGCTTATCTACAATCATATCACAAC CAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCTTCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGA GATATAGTAGATCGTGGAAGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACGTGAAGTGCGTCG TGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCCAATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCA TGACTACCATGGCATTCTGGTTGATGAAGAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGCTC GTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGATCCAGGTTCGAACTCTGGCCAGTGCAGGAGGAC CAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAAGCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCA GATTGGTGAGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAAGTCT AAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCCTTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTC AGAAGCAGCAGCGGGAGAAGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAAGTCGAAGACTAA GTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAAC AAGATCCGAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATGGACAGGAGCCTCGTCCAGGGAGAGC TGGTGACGGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCCACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGA GCTGGAGGAGTACCGCAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAGAGTCCTCCAGACCAG CCTGCGGTCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGACCTTGTGCCGGAGCCGACTACTGGAGATGACAGTGATGCTGCCACCTTTA AGCCAACTCTCCCCGATCTGTCCCCTGATGAACCTTCAGAAGCACTCGGCTTCCCAATGTTAGAGAAGGAGGAGGAAGCCCATAGACCCCCAAGCCCCAC TGAGGCCCCTACTGAGGCCAGCCCCGAGCCAGCCCCAGACCCAGCCCCGGTGGCTGAAGAGGCTGCCCCCTCAGCTGTCGAGGAGGGGGCCGCCGCGGAC CCTGGCAGCGATGGGTCTCCAGGCAAGTCCCCGTCCAAAAAGAAGAAGAAGTTCCGTACCCCGTCCTTTCTGAAGAAGAGCAAGAAGAAGAGTGACTCCT GAAAGCCCTGCGCTAACACTGTCCTGTCCGGAGCGACCCTGGCTCTGCCAGCGTCCCCGGCCACGTCTGTGCTCTGTCCTTGTGTAATGGAATGCAAAAA AGCCAAGCCCTCCGCCTAGAGGTCCCCTCACGTGACCAGCCCCGTGTAGCCCCGGGCTGACCCAGTGTGTGCTCAGCAGCCCCACCCCACCCTGCCCCTT GTCCTCTCAGAGCCTCAGCTTCTGGGGGAGACATGCTCTCCCCACAGGGGGGAGGCACTAAGTCATGGTCCTGGCTGGAAGGTACTGAAGGCTTCTGCAG CTTTGGCTGCACGTCACCCTCCTGAGCCTCACCTTTCCTGCCGTCCCTCCTGTTGTGAAATCACCACATTCTGTCTCTGCTTGGCTTCCCCTCCACCCTA AAGTCTCAGGTGACGGACTCAGACTCCTGGCTTCATGTGGCATTCTCTCTGCTCAGTGATCTCACTTAAATCTATATACAAAGCCTTGGTCCCGTGAAAA CACTCGTGTGCCCACCAGCGGCCTTGAAGAGGCAGGTCTGGGCCAGATGCTGGGCAGGAAACCCCAGCGGCAGATGGGCCTGTGTGCACCCAACGTGATG CTATGCATGTCTGACCGACGATCCCTCGACCAGAATCAGATTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCTCTGGATTCCTGTCATAGGGAAGGTATA TCAGGAGGGGAAGAGGCCTTTCTAGAATTTTCTTTGAGCAGGTTTACAATTTAGCTTACATTTTTCGACTGTGAACGTGAATAGGCTGCTTTTTGCTTTC TTCTTTCCAGACCCCACAGTAGAGCACTTTTCACTTATTTGGGGGAGGCTTCAGGGGACTGTTCTCACCTTAACTCAGCCAGAAAGATGCCCTAGTTGTG ATCAAAGGTAACTCGAGGTGGAGGGTAGCCCTGGGGCCCCTCGACATCACCGTCATTGATGGAGCCTGAACCGTGTGCTCCTCGGCAGATGCTGTTGTTG TTACTTCCCTCCAAGAGGCTGGAAAAGGGCTCAGAGCTGCTGAGCAGGAACCGGAGGGTGACCCATTTCAGGAGGTGCCGGTACCAGCCTGACTAGGTAC AGGCAAGCTTGTGTGGGCCCAACAGGCCCTTGGTAGAGCTGGTGCCAGATGTGGGCTCAGATCCTGGGCATGATGGGCCGAGCCACCTCGGATCCCACTG ATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTGACCGCCGCTTCCAGCTTCCTCTGAGCCGCAGGGCCTGCTACGCGGGCAAGCGTGCTGCC TCTCTTCTGTGTCGTTTTGTTGCCAAGGCAGAATGAAAAGTCCTTAACCGTGGACTCTTCCTTTATCCCCTCCTTTACCCCACATATGCAATGACTTTTA ATTTTCACTTTTGTAGTTTAATCCTTTGTATTACAACATGAAATATAGTTGCATATATGGACACCGACTTGGGAGGACAGGTCCTGAATGTCCTTTCTCC AGTGTAACATGTTTTACTCACAAATAAAATTCTTTCAGCAA

refseq_ADD1.F1 (forward primer)
refseq_ADD1.R1 (reverse primer)
Alternative donor

Frame 0

APRSGGGGERRG*GAERPGGPLLRARGRGRSRS RSRRAVAALGPRNPARCPRFASEEPRKIVQ*MVI LVLRW*PHHPRPQPLTRRGTSTE*MRTTQST*G RGTWHQTFARTST*WSKRRGCP*FCKALLSVKN WNQ*YRSNLRRGRTPQAYWHYSRLQIL*PRMYQM STQQLRKEGWLP*T*VLVW*LL*TILEDLILLR MTKERSYYGVNWQRFID*QISLGGLSLSTIISQ PE*TPSRNTSSLSLLGFFTVK*LHPVWLRSIYKE I**IVEALIWE*IRPASPYTLQFMLHART*SAS CTFTPQQGLRSLQ*NVASCQSPRRRFPLEKWLI MTTMAFWLMKRKKF*FRKIWGLKARFLFSGTMGS CQLERALRRPSITSITLWLPVRSRFELWPVQED QTT*SC*ILRSTKPSPVPQGLR*GKALDRLPSG RLVSRNLKPSCGCSIIWATELATLIDTLL*ERSL KNTAMWRFLLVSQVTPLLVTVIRALAPHSDTVF RSSSGRRQDG*TLAGATKLPRKGRMEAVPSRRL SGLKRMDIELPPLLSLTCLFH*TLTQKRSRR*GT RSESRIYRTLRRLALSPRFCVV**WTGASSRES W*RPPRPSLKRSTSPTSL*APRAPTPSPHSQTV SWRSTAGRWRGSRRALKRIWTRLENRKKRVLQTS LRSPTRLPALPSSWRKTLCRSRLLEMTVMLPPL SQLSPICPLMNLQKHSASQC*RRRRKPIDPQAP LRPLLRPAPSQPQTQPRWLKRLPPQLSRRGPPRT LAAMGLQASPRPKRRRSSVPRPF*RRARRRVTP ESPALTLSCPERPWLCQRPRPRLCSVLV*WNAK KPSPPPRGPLT*PAPCSPGLTQCVLSSPTPPCPL SSQSLSFWGRHALPTGGRH*VMVLAGRY*RLLQ LWLHVTLLSLTFPAVPPVVKSPHSVSAWLPLHP KVSGDGLRLLASCGILSAQ*SHLNLYTKPWSREN TRVPTSGLEEAGLGQMLGRKPQRQMGLCAPNVM LCMSDRRSLDQNQIQELSFFFTWVSGFLS*GRY IRRGRGLSRIFFEQVYNLAYIFRL*T*IGCFLLS SFQTPQ*STFHLFGGGFRGLFSP*LSQKDALVV IKGNSRWRVALGPLDITVIDGA*TVCSSADAVV VTSLQEAGKGLRAAEQEPEGDPFQEVPVPA*LGT GKLVWAQQALGRAGARCGLRSWA*WAEPPRIPL IGQPSENQAAAWH*PPLPASSEPQGLLRGQACC LSSVSFCCQGRMKSP*PWTLPLSPPLPHICNDF* FSLL*FNPLYYNMKYSCIYGHRLGRTGPECPFS SVTCFTHK*NSFS

Frame 1

HPGRAVGASGGAEGRRGLAGRCCGPGDGGGAGA GADGRWPHWDPGIPRAAHDSLLRNLERLYNEW* FSCCGGDLTTPDHSPSQGEVLRPSR*EQPRVLEG EEHGTRPSPGLQHDGAKEEGVHDSAKPCFL*RI GINDTGAI*EGEEPHRPIGITADCRFYDHECTK CLPSSSARRDGCLKHESWYGDSCERS*RI*FYCV *QRREVITV*IGSVL*TSRSLWVVSAYLQSYHN QSELRAGTLPHCPFWASLQ*SDCIQFG*DQSTR RYSRSWKH*SGSESGRLHLTLCNLCCTPGREVRR AHSHPSRGCGLCNEMWPLANLPGGAFPWRSGLS *LPWHSG**RGKSFDSEKSGA*KQGSYSPEPWA RVSWRER*GGLLLHP*PCGCL*DPGSNSGQCRRT RQLSPAES*EVQSQVPFPRVSGRGRHWIASQVA DW*AGI*SPHADAR*SGLQNWLPLSIPCSEREV *KIQRCGGSC*CHRLLLC**R*FGHLLPTQTQFS EAAAGEDKMAELWPGRRSFRGRAEWKQSQVED* VD*RGWT*NFHLCCP*PVCSIEH*PKRGPGDEE QDPRAEFTGH*DGWPSVPGFVWCSDGQEPRPGRA GDGLQGHH*KGVPAPRHCEHHGPQPLHHTHRP* AGGVPQGGGEEAEGL*RESGRG*RTERKESSRP ACGPPPASQHSHQAGGRPCAGADYWR*Q*CCHL* ANSPRSVP**TFRSTRLPNVREGGGSP*TPKPH *GPY*GQPRASPRPSPGG*RGCPLSCRGGGRRG PWQRWVSRQVPVQKEEEVPYPVLSEEEQEEE*LL KALR*HCPVRSDPGSASVPGHVCALSLCNGMQK SQALRLEVPSRDQPRVAPG*PSVCSAAPPHPAP CPLRASASGGDMLSPQGGGTKSWSWLEGTEGFCS FGCTSPS*ASPFLPSLLL*NHHILSLLGFPSTL KSQVTDSDSWLHVAFSLLSDLT*IYIQSLGPVK TLVCPPAALKRQVWARCWAGNPSGRWACVHPT*C YACLTDDPSTRIRFRSSVSFSLGSLDSCHREGI SGGEEAFLEFSLSRFTI*LTFFDCERE*AAFCF LLSRPHSRALFTYLGEASGDCSHLNSARKMP*L* SKVTRGGG*PWGPSTSPSLMEPEPCAPRQMLLL LLPSKRLEKGSELLSRNRRVTHFRRCRYQPD*V QASLCGPNRPLVELVPDVGSDPGHDGPSHLGSH* LASRARTRLLHGTDRRFQLPLSRRACYAGKRAA SLLCRFVAKAE*KVLNRGLFLYPLLYPTYAMTF NFHFCSLILCITT*NIVAYMDTDLGGQVLNVLSP V*HVLLTNKILSA

Frame 2

TQVGRWGRAEGLRGGEAWRAAAAGQGTGAEPEP EPTGGGRTGTPESRALPTIRF*GT*KDCTMNGD SRAAVVTSPPPTTAPHKERYFDRVDENNPEYLR ERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEEL ESMIQEQFKKGKNPTGLLALQQIADFMTTNVPN VYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIA YDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITT RVNSEQEHFLIVPFGLLYSEVTASSLVKINLQG DIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCV VHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYH DYHGILVDEEEKVLIQKNLGPKSKVLILRNHGL VSVGESVEEAFYYIHNLVVACEIQVRTLASAGG PDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQ IGEQEFEALMRMLDNLGYRTGYPYRYPALREKS KKYSDVEVPASVTGYSFASDGDSGTCSPLRHSF QKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTK WTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRN KIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGE LVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRE LEEYRREVERKQKGSEENLDEAREQKEKSPPDQ PAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATF KPTLPDLSPDEPSEALGFPMLEKEEEAHRPPSPT EAPTEASPEPAPDPAPVAEEAAPSAVEEGAAAD PGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS *KPCANTVLSGATLALPASPATSVLCPCVMECKK AKPSA*RSPHVTSPV*PRADPVCAQQPHPTLPL VLSEPQLLGETCSPHRGEALSHGPGWKVLKASA ALAARHPPEPHLSCRPSCCEITTFCLCLASPPP* SLR*RTQTPGFMWHSLCSVISLKSIYKALVP*K HSCAHQRP*RGRSGPDAGQETPAADGPVCTQRD AMHV*PTIPRPESDSGAQFLFHLGLWIPVIGKVY QEGKRPF*NFL*AGLQFSLHFSTVNVNRLLFAF FFPDPTVEHFSLIWGRLQGTVLTLTQPERCPSC DQR*LEVEGSPGAPRHHRH*WSLNRVLLGRCCCC YFPPRGWKRAQSC*AGTGG*PISGGAGTSLTRY RQACVGPTGPW*SWCQMWAQILGMMGRATSDPT DWPAEREPGCCMALTAASSFL*AAGPATRASVLP LFCVVLLPRQNEKSLTVDSSFIPSFTPHMQ*LL IFTFVV*SFVLQHEI*LHIWTPTWEDRS*MSFL QCNMFYSQIKFFQQ

Sequence NM_014190

GCACCCAGGTCGGGCGGTGGGGGCGAGCGGAGGGGCTGAGGGGCGGAGAGGCCTGGCGGGCCGCTGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCC GGAGCCGACGGGCGGTGGCCGCACTGGGACCCCGGAATCCCGCGCGCTGCCCACGATTCGCTTCTGAGGAACCTAGAAAGATTGTACAATGAATGGTGAT TCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGTACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGG AGAGGAACATGGCACCAGACCTTCGCCAGGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAATT GGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGATTGCAGATTTTATGACCACGAATGTACCAAAT GTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTTAAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGT ATGACAAAGGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGCTTATCTACAATCATATCACAAC CAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCTTCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGA GATATAGTAGATCGTGGAAGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACGTGAAGTGCGTCG TGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCCAATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCA TGACTACCATGGCATTCTGGTTGATGAAGAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGCTC GTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGATCCAGGTTCGAACTCTGGCCAGTGCAGGAGGAC CAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAAGCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCA GATTGGTGAGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAAGTCT AAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCCTTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTC AGAAGCAGCAGCGGGAGAAGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAAGTCGAAGACTAA GTGGACTAAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAAC AAGATCCGAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATGGACAGGAGCCTCGTCCAGGGAGAGC TGGTGACGGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCCACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGA GCTGGAGGAGTACCGCAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAGAGTCCTCCAGACCAG CCTGCGGTCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGGAGACGGATGCGCTAGAGAGTACCTGTTACCCTAACCTTGTGCCGGAGCCG ACTACTGGAGATGACAGTGATGCTGCCACCTTTAAGCCAACTCTCCCCGATCTGTCCCCTGATGAACCTTCAGAAGCACTCGGCTTCCCAATGTTAGAGA AGGAGGAGGAAGCCCATAGACCCCCAAGCCCCACTGAGGCCCCTACTGAGGCCAGCCCCGAGCCAGCCCCAGACCCAGCCCCGGTGGCTGAAGAGGCTGC CCCCTCAGCTGTCGAGGAGGGGGCCGCCGCGGACCCTGGCAGCGATGGGTCTCCAGGCAAGTCCCCGTCCAAAAAGAAGAAGAAGTTCCGTACCCCGTCC TTTCTGAAGAAGAGCAAGAAGAAGAGTGACTCCTGAAAGCCCTGCGCTAACACTGTCCTGTCCGGAGCGACCCTGGCTCTGCCAGCGTCCCCGGCCACGT CTGTGCTCTGTCCTTGTGTAATGGAATGCAAAAAAGCCAAGCCCTCCGCCTAGAGGTCCCCTCACGTGACCAGCCCCGTGTAGCCCCGGGCTGACCCAGT GTGTGCTCAGCAGCCCCACCCCACCCTGCCCCTTGTCCTCTCAGAGCCTCAGCTTCTGGGGGAGACATGCTCTCCCCACAGGGGGGAGGCACTAAGTCAT GGTCCTGGCTGGAAGGTACTGAAGGCTTCTGCAGCTTTGGCTGCACGTCACCCTCCTGAGCCTCACCTTTCCTGCCGTCCCTCCTGTTGTGAAATCACCA CATTCTGTCTCTGCTTGGCTTCCCCTCCACCCTAAAGTCTCAGGTGACGGACTCAGACTCCTGGCTTCATGTGGCATTCTCTCTGCTCAGTGATCTCACT TAAATCTATATACAAAGCCTTGGTCCCGTGAAAACACTCGTGTGCCCACCAGCGGCCTTGAAGAGGCAGGTCTGGGCCAGATGCTGGGCAGGAAACCCCA GCGGCAGATGGGCCTGTGTGCACCCAACGTGATGCTATGCATGTCTGACCGACGATCCCTCGACCAGAATCAGATTCAGGAGCTCAGTTTCTTTTTCACT TGGGTCTCTGGATTCCTGTCATAGGGAAGGTATATCAGGAGGGGAAGAGGCCTTTCTAGAATTTTCTTTGAGCAGGTTTACAATTTAGCTTACATTTTTC GACTGTGAACGTGAATAGGCTGCTTTTTGCTTTCTTCTTTCCAGACCCCACAGTAGAGCACTTTTCACTTATTTGGGGGAGGCTTCAGGGGACTGTTCTC ACCTTAACTCAGCCAGAAAGATGCCCTAGTTGTGATCAAAGGTAACTCGAGGTGGAGGGTAGCCCTGGGGCCCCTCGACATCACCGTCATTGATGGAGCC TGAACCGTGTGCTCCTCGGCAGATGCTGTTGTTGTTACTTCCCTCCAAGAGGCTGGAAAAGGGCTCAGAGCTGCTGAGCAGGAACCGGAGGGTGACCCAT TTCAGGAGGTGCCGGTACCAGCCTGACTAGGTACAGGCAAGCTTGTGTGGGCCCAACAGGCCCTTGGTAGAGCTGGTGCCAGATGTGGGCTCAGATCCTG GGCATGATGGGCCGAGCCACCTCGGATCCCACTGATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTGACCGCCGCTTCCAGCTTCCTCTGAG CCGCAGGGCCTGCTACGCGGGCAAGCGTGCTGCCTCTCTTCTGTGTCGTTTTGTTGCCAAGGCAGAATGAAAAGTCCTTAACCGTGGACTCTTCCTTTAT CCCCTCCTTTACCCCACATATGCAATGACTTTTAATTTTCACTTTTGTAGTTTAATCCTTTGTATTACAACATGAAATATAGTTGCATATATGGACACCG ACTTGGGAGGACAGGTCCTGAATGTCCTTTCTCCAGTGTAACATGTTTTACTCACAAATAAAATTCTTTCAGCAA

refseq_ADD1.F1 (forward primer)
refseq_ADD1.R1 (reverse primer)
Alternative donor

Frame 0

APRSGGGGERRG*GAERPGGPLLRARGRGRSRS RSRRAVAALGPRNPARCPRFASEEPRKIVQ*MVI LVLRW*PHHPRPQPLTRRGTSTE*MRTTQST*G RGTWHQTFARTST*WSKRRGCP*FCKALLSVKN WNQ*YRSNLRRGRTPQAYWHYSRLQIL*PRMYQM STQQLRKEGWLP*T*VLVW*LL*TILEDLILLR MTKERSYYGVNWQRFID*QISLGGLSLSTIISQ PE*TPSRNTSSLSLLGFFTVK*LHPVWLRSIYKE I**IVEALIWE*IRPASPYTLQFMLHART*SAS CTFTPQQGLRSLQ*NVASCQSPRRRFPLEKWLI MTTMAFWLMKRKKF*FRKIWGLKARFLFSGTMGS CQLERALRRPSITSITLWLPVRSRFELWPVQED QTT*SC*ILRSTKPSPVPQGLR*GKALDRLPSG RLVSRNLKPSCGCSIIWATELATLIDTLL*ERSL KNTAMWRFLLVSQVTPLLVTVIRALAPHSDTVF RSSSGRRQDG*TLAGATKLPRKGRMEAVPSRRL SGLKRMDIELPPLLSLTCLFH*TLTQKRSRR*GT RSESRIYRTLRRLALSPRFCVV**WTGASSRES W*RPPRPSLKRSTSPTSL*APRAPTPSPHSQTV SWRSTAGRWRGSRRALKRIWTRLENRKKRVLQTS LRSPTRLPALPSSWRKETDALESTCYPNLVPEP TTGDDSDAATFKPTLPDLSPDEPSEALGFPMLE KEEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAA PSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPS FLKKSKKKSDS*KPCANTVLSGATLALPASPAT SVLCPCVMECKKAKPSA*RSPHVTSPV*PRADPV CAQQPHPTLPLVLSEPQLLGETCSPHRGEALSH GPGWKVLKASAALAARHPPEPHLSCRPSCCEIT TFCLCLASPPP*SLR*RTQTPGFMWHSLCSVISL KSIYKALVP*KHSCAHQRP*RGRSGPDAGQETP AADGPVCTQRDAMHV*PTIPRPESDSGAQFLFH LGLWIPVIGKVYQEGKRPF*NFL*AGLQFSLHFS TVNVNRLLFAFFFPDPTVEHFSLIWGRLQGTVL TLTQPERCPSCDQR*LEVEGSPGAPRHHRH*WS LNRVLLGRCCCCYFPPRGWKRAQSC*AGTGG*PI SGGAGTSLTRYRQACVGPTGPW*SWCQMWAQIL GMMGRATSDPTDWPAEREPGCCMALTAASSFL* AAGPATRASVLPLFCVVLLPRQNEKSLTVDSSFI PSFTPHMQ*LLIFTFVV*SFVLQHEI*LHIWTP TWEDRS*MSFLQCNMFYSQIKFFQQ

Frame 1

HPGRAVGASGGAEGRRGLAGRCCGPGDGGGAGA GADGRWPHWDPGIPRAAHDSLLRNLERLYNEW* FSCCGGDLTTPDHSPSQGEVLRPSR*EQPRVLEG EEHGTRPSPGLQHDGAKEEGVHDSAKPCFL*RI GINDTGAI*EGEEPHRPIGITADCRFYDHECTK CLPSSSARRDGCLKHESWYGDSCERS*RI*FYCV *QRREVITV*IGSVL*TSRSLWVVSAYLQSYHN QSELRAGTLPHCPFWASLQ*SDCIQFG*DQSTR RYSRSWKH*SGSESGRLHLTLCNLCCTPGREVRR AHSHPSRGCGLCNEMWPLANLPGGAFPWRSGLS *LPWHSG**RGKSFDSEKSGA*KQGSYSPEPWA RVSWRER*GGLLLHP*PCGCL*DPGSNSGQCRRT RQLSPAES*EVQSQVPFPRVSGRGRHWIASQVA DW*AGI*SPHADAR*SGLQNWLPLSIPCSEREV *KIQRCGGSC*CHRLLLC**R*FGHLLPTQTQFS EAAAGEDKMAELWPGRRSFRGRAEWKQSQVED* VD*RGWT*NFHLCCP*PVCSIEH*PKRGPGDEE QDPRAEFTGH*DGWPSVPGFVWCSDGQEPRPGRA GDGLQGHH*KGVPAPRHCEHHGPQPLHHTHRP* AGGVPQGGGEEAEGL*RESGRG*RTERKESSRP ACGPPPASQHSHQAGGRRRMR*RVPVTLTLCRSR LLEMTVMLPPLSQLSPICPLMNLQKHSASQC*R RRRKPIDPQAPLRPLLRPAPSQPQTQPRWLKRL PPQLSRRGPPRTLAAMGLQASPRPKRRRSSVPRP F*RRARRRVTPESPALTLSCPERPWLCQRPRPR LCSVLV*WNAKKPSPPPRGPLT*PAPCSPGLTQ CVLSSPTPPCPLSSQSLSFWGRHALPTGGRH*VM VLAGRY*RLLQLWLHVTLLSLTFPAVPPVVKSP HSVSAWLPLHPKVSGDGLRLLASCGILSAQ*SH LNLYTKPWSRENTRVPTSGLEEAGLGQMLGRKPQ RQMGLCAPNVMLCMSDRRSLDQNQIQELSFFFT WVSGFLS*GRYIRRGRGLSRIFFEQVYNLAYIF RL*T*IGCFLLSSFQTPQ*STFHLFGGGFRGLFS P*LSQKDALVVIKGNSRWRVALGPLDITVIDGA *TVCSSADAVVVTSLQEAGKGLRAAEQEPEGDP FQEVPVPA*LGTGKLVWAQQALGRAGARCGLRSW A*WAEPPRIPLIGQPSENQAAAWH*PPLPASSE PQGLLRGQACCLSSVSFCCQGRMKSP*PWTLPL SPPLPHICNDF*FSLL*FNPLYYNMKYSCIYGHR LGRTGPECPFSSVTCFTHK*NSFS

Frame 2

TQVGRWGRAEGLRGGEAWRAAAAGQGTGAEPEP EPTGGGRTGTPESRALPTIRF*GT*KDCTMNGD SRAAVVTSPPPTTAPHKERYFDRVDENNPEYLR ERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEEL ESMIQEQFKKGKNPTGLLALQQIADFMTTNVPN VYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIA YDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITT RVNSEQEHFLIVPFGLLYSEVTASSLVKINLQG DIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCV VHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYH DYHGILVDEEEKVLIQKNLGPKSKVLILRNHGL VSVGESVEEAFYYIHNLVVACEIQVRTLASAGG PDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQ IGEQEFEALMRMLDNLGYRTGYPYRYPALREKS KKYSDVEVPASVTGYSFASDGDSGTCSPLRHSF QKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTK WTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRN KIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGE LVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRE LEEYRREVERKQKGSEENLDEAREQKEKSPPDQ PAVPHPPPSTPIKLEEGDGCAREYLLP*PCAGA DYWR*Q*CCHL*ANSPRSVP**TFRSTRLPNVRE GGGSP*TPKPH*GPY*GQPRASPRPSPGG*RGC PLSCRGGGRRGPWQRWVSRQVPVQKEEEVPYPV LSEEEQEEE*LLKALR*HCPVRSDPGSASVPGHV CALSLCNGMQKSQALRLEVPSRDQPRVAPG*PS VCSAAPPHPAPCPLRASASGGDMLSPQGGGTKS WSWLEGTEGFCSFGCTSPS*ASPFLPSLLL*NHH ILSLLGFPSTLKSQVTDSDSWLHVAFSLLSDLT *IYIQSLGPVKTLVCPPAALKRQVWARCWAGNP SGRWACVHPT*CYACLTDDPSTRIRFRSSVSFSL GSLDSCHREGISGGEEAFLEFSLSRFTI*LTFF DCERE*AAFCFLLSRPHSRALFTYLGEASGDCS HLNSARKMP*L*SKVTRGGG*PWGPSTSPSLMEP EPCAPRQMLLLLLPSKRLEKGSELLSRNRRVTH FRRCRYQPD*VQASLCGPNRPLVELVPDVGSDP GHDGPSHLGSH*LASRARTRLLHGTDRRFQLPLS RRACYAGKRAASLLCRFVAKAE*KVLNRGLFLY PLLYPTYAMTFNFHFCSLILCITT*NIVAYMDT DLGGQVLNVLSPV*HVLLTNKILSA

Sequence NM_014189

GCACCCAGGTCGGGCGGTGGGGGCGAGCGGAGGGGCTGAGGGGCGGAGAGGCCTGGCGGGCCGCTGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCC GGAGCCGACGGGCGGTGGCCGCACTGGGACCCCGGAATCCCGCGCGCTGCCCACGATTCGCTTCTGAGGAACCTAGAAAGATTGTACAATGAATGGTGAT TCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGTACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGG AGAGGAACATGGCACCAGACCTTCGCCAGGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAATT GGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGATTGCAGATTTTATGACCACGAATGTACCAAAT GTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTTAAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGT ATGACAAAGGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGCTTATCTACAATCATATCACAAC CAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCTTCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGA GATATAGTAGATCGTGGAAGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACGTGAAGTGCGTCG TGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCCAATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCA TGACTACCATGGCATTCTGGTTGATGAAGAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGCTC GTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGATCCAGGTTCGAACTCTGGCCAGTGCAGGAGGAC CAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAAGCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCA GATTGGTGAGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAAGTCT AAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCCTTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTC AGAAGCAGCAGCGGGAGAAGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAAGTCGAAGACTAA GGTGTGGACGAACATTACACACGATCACGTGAAACCCTTGCTGCAGTCTCTCTCGTCCGGTGTCTGCGTGCCAAGCTGTATTACCAACTGCTTGTGGACT AAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATCC GAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATGGACAGGAGCCTCGTCCAGGGAGAGCTGGTGAC GGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCCACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGAGCTGGAG GAGTACCGCAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAGAGTCCTCCAGACCAGCCTGCGG TCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGACCTTGTGCCGGAGCCGACTACTGGAGATGACAGTGATGCTGCCACCTTTAAGCCAAC TCTCCCCGATCTGTCCCCTGATGAACCTTCAGAAGCACTCGGCTTCCCAATGTTAGAGAAGGAGGAGGAAGCCCATAGACCCCCAAGCCCCACTGAGGCC CCTACTGAGGCCAGCCCCGAGCCAGCCCCAGACCCAGCCCCGGTGGCTGAAGAGGCTGCCCCCTCAGCTGTCGAGGAGGGGGCCGCCGCGGACCCTGGCA GCGATGGGTCTCCAGGCAAGTCCCCGTCCAAAAAGAAGAAGAAGTTCCGTACCCCGTCCTTTCTGAAGAAGAGCAAGAAGAAGAGTGACTCCTGAAAGCC CTGCGCTAACACTGTCCTGTCCGGAGCGACCCTGGCTCTGCCAGCGTCCCCGGCCACGTCTGTGCTCTGTCCTTGTGTAATGGAATGCAAAAAAGCCAAG CCCTCCGCCTAGAGGTCCCCTCACGTGACCAGCCCCGTGTAGCCCCGGGCTGACCCAGTGTGTGCTCAGCAGCCCCACCCCACCCTGCCCCTTGTCCTCT CAGAGCCTCAGCTTCTGGGGGAGACATGCTCTCCCCACAGGGGGGAGGCACTAAGTCATGGTCCTGGCTGGAAGGTACTGAAGGCTTCTGCAGCTTTGGC TGCACGTCACCCTCCTGAGCCTCACCTTTCCTGCCGTCCCTCCTGTTGTGAAATCACCACATTCTGTCTCTGCTTGGCTTCCCCTCCACCCTAAAGTCTC AGGTGACGGACTCAGACTCCTGGCTTCATGTGGCATTCTCTCTGCTCAGTGATCTCACTTAAATCTATATACAAAGCCTTGGTCCCGTGAAAACACTCGT GTGCCCACCAGCGGCCTTGAAGAGGCAGGTCTGGGCCAGATGCTGGGCAGGAAACCCCAGCGGCAGATGGGCCTGTGTGCACCCAACGTGATGCTATGCA TGTCTGACCGACGATCCCTCGACCAGAATCAGATTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCTCTGGATTCCTGTCATAGGGAAGGTATATCAGGAG GGGAAGAGGCCTTTCTAGAATTTTCTTTGAGCAGGTTTACAATTTAGCTTACATTTTTCGACTGTGAACGTGAATAGGCTGCTTTTTGCTTTCTTCTTTC CAGACCCCACAGTAGAGCACTTTTCACTTATTTGGGGGAGGCTTCAGGGGACTGTTCTCACCTTAACTCAGCCAGAAAGATGCCCTAGTTGTGATCAAAG GTAACTCGAGGTGGAGGGTAGCCCTGGGGCCCCTCGACATCACCGTCATTGATGGAGCCTGAACCGTGTGCTCCTCGGCAGATGCTGTTGTTGTTACTTC CCTCCAAGAGGCTGGAAAAGGGCTCAGAGCTGCTGAGCAGGAACCGGAGGGTGACCCATTTCAGGAGGTGCCGGTACCAGCCTGACTAGGTACAGGCAAG CTTGTGTGGGCCCAACAGGCCCTTGGTAGAGCTGGTGCCAGATGTGGGCTCAGATCCTGGGCATGATGGGCCGAGCCACCTCGGATCCCACTGATTGGCC AGCCGAGCGAGAACCAGGCTGCTGCATGGCACTGACCGCCGCTTCCAGCTTCCTCTGAGCCGCAGGGCCTGCTACGCGGGCAAGCGTGCTGCCTCTCTTC TGTGTCGTTTTGTTGCCAAGGCAGAATGAAAAGTCCTTAACCGTGGACTCTTCCTTTATCCCCTCCTTTACCCCACATATGCAATGACTTTTAATTTTCA CTTTTGTAGTTTAATCCTTTGTATTACAACATGAAATATAGTTGCATATATGGACACCGACTTGGGAGGACAGGTCCTGAATGTCCTTTCTCCAGTGTAA CATGTTTTACTCACAAATAAAATTCTTTCAGCAA

refseq_ADD1.F1 (forward primer)
refseq_ADD1.R1 (reverse primer)
Alternative donor

Frame 0

APRSGGGGERRG*GAERPGGPLLRARGRGRSRS RSRRAVAALGPRNPARCPRFASEEPRKIVQ*MVI LVLRW*PHHPRPQPLTRRGTSTE*MRTTQST*G RGTWHQTFARTST*WSKRRGCP*FCKALLSVKN WNQ*YRSNLRRGRTPQAYWHYSRLQIL*PRMYQM STQQLRKEGWLP*T*VLVW*LL*TILEDLILLR MTKERSYYGVNWQRFID*QISLGGLSLSTIISQ PE*TPSRNTSSLSLLGFFTVK*LHPVWLRSIYKE I**IVEALIWE*IRPASPYTLQFMLHART*SAS CTFTPQQGLRSLQ*NVASCQSPRRRFPLEKWLI MTTMAFWLMKRKKF*FRKIWGLKARFLFSGTMGS CQLERALRRPSITSITLWLPVRSRFELWPVQED QTT*SC*ILRSTKPSPVPQGLR*GKALDRLPSG RLVSRNLKPSCGCSIIWATELATLIDTLL*ERSL KNTAMWRFLLVSQVTPLLVTVIRALAPHSDTVF RSSSGRRQDG*TLAGATKLPRKGRMEAVPSRRL RCGRTLHTIT*NPCCSLSRPVSACQAVLPTACGL KRMDIELPPLLSLTCLFH*TLTQKRSRR*GTRS ESRIYRTLRRLALSPRFCVV**WTGASSRESW* RPPRPSLKRSTSPTSL*APRAPTPSPHSQTVSWR STAGRWRGSRRALKRIWTRLENRKKRVLQTSLR SPTRLPALPSSWRKTLCRSRLLEMTVMLPPLSQ LSPICPLMNLQKHSASQC*RRRRKPIDPQAPLRP LLRPAPSQPQTQPRWLKRLPPQLSRRGPPRTLA AMGLQASPRPKRRRSSVPRPF*RRARRRVTPES PALTLSCPERPWLCQRPRPRLCSVLV*WNAKKPS PPPRGPLT*PAPCSPGLTQCVLSSPTPPCPLSS QSLSFWGRHALPTGGRH*VMVLAGRY*RLLQLW LHVTLLSLTFPAVPPVVKSPHSVSAWLPLHPKVS GDGLRLLASCGILSAQ*SHLNLYTKPWSRENTR VPTSGLEEAGLGQMLGRKPQRQMGLCAPNVMLC MSDRRSLDQNQIQELSFFFTWVSGFLS*GRYIRR GRGLSRIFFEQVYNLAYIFRL*T*IGCFLLSSF QTPQ*STFHLFGGGFRGLFSP*LSQKDALVVIK GNSRWRVALGPLDITVIDGA*TVCSSADAVVVTS LQEAGKGLRAAEQEPEGDPFQEVPVPA*LGTGK LVWAQQALGRAGARCGLRSWA*WAEPPRIPLIG QPSENQAAAWH*PPLPASSEPQGLLRGQACCLSS VSFCCQGRMKSP*PWTLPLSPPLPHICNDF*FS LL*FNPLYYNMKYSCIYGHRLGRTGPECPFSSV TCFTHK*NSFS

Frame 1

HPGRAVGASGGAEGRRGLAGRCCGPGDGGGAGA GADGRWPHWDPGIPRAAHDSLLRNLERLYNEW* FSCCGGDLTTPDHSPSQGEVLRPSR*EQPRVLEG EEHGTRPSPGLQHDGAKEEGVHDSAKPCFL*RI GINDTGAI*EGEEPHRPIGITADCRFYDHECTK CLPSSSARRDGCLKHESWYGDSCERS*RI*FYCV *QRREVITV*IGSVL*TSRSLWVVSAYLQSYHN QSELRAGTLPHCPFWASLQ*SDCIQFG*DQSTR RYSRSWKH*SGSESGRLHLTLCNLCCTPGREVRR AHSHPSRGCGLCNEMWPLANLPGGAFPWRSGLS *LPWHSG**RGKSFDSEKSGA*KQGSYSPEPWA RVSWRER*GGLLLHP*PCGCL*DPGSNSGQCRRT RQLSPAES*EVQSQVPFPRVSGRGRHWIASQVA DW*AGI*SPHADAR*SGLQNWLPLSIPCSEREV *KIQRCGGSC*CHRLLLC**R*FGHLLPTQTQFS EAAAGEDKMAELWPGRRSFRGRAEWKQSQVED* GVDEHYTRSRETLAAVSLVRCLRAKLYYQLLVD *RGWT*NFHLCCP*PVCSIEH*PKRGPGDEEQDP RAEFTGH*DGWPSVPGFVWCSDGQEPRPGRAGD GLQGHH*KGVPAPRHCEHHGPQPLHHTHRP*AG GVPQGGGEEAEGL*RESGRG*RTERKESSRPACG PPPASQHSHQAGGRPCAGADYWR*Q*CCHL*AN SPRSVP**TFRSTRLPNVREGGGSP*TPKPH*G PY*GQPRASPRPSPGG*RGCPLSCRGGGRRGPWQ RWVSRQVPVQKEEEVPYPVLSEEEQEEE*LLKA LR*HCPVRSDPGSASVPGHVCALSLCNGMQKSQ ALRLEVPSRDQPRVAPG*PSVCSAAPPHPAPCPL RASASGGDMLSPQGGGTKSWSWLEGTEGFCSFG CTSPS*ASPFLPSLLL*NHHILSLLGFPSTLKS QVTDSDSWLHVAFSLLSDLT*IYIQSLGPVKTLV CPPAALKRQVWARCWAGNPSGRWACVHPT*CYA CLTDDPSTRIRFRSSVSFSLGSLDSCHREGISG GEEAFLEFSLSRFTI*LTFFDCERE*AAFCFLLS RPHSRALFTYLGEASGDCSHLNSARKMP*L*SK VTRGGG*PWGPSTSPSLMEPEPCAPRQMLLLLL PSKRLEKGSELLSRNRRVTHFRRCRYQPD*VQAS LCGPNRPLVELVPDVGSDPGHDGPSHLGSH*LA SRARTRLLHGTDRRFQLPLSRRACYAGKRAASL LCRFVAKAE*KVLNRGLFLYPLLYPTYAMTFNFH FCSLILCITT*NIVAYMDTDLGGQVLNVLSPV* HVLLTNKILSA

Frame 2

TQVGRWGRAEGLRGGEAWRAAAAGQGTGAEPEP EPTGGGRTGTPESRALPTIRF*GT*KDCTMNGD SRAAVVTSPPPTTAPHKERYFDRVDENNPEYLR ERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEEL ESMIQEQFKKGKNPTGLLALQQIADFMTTNVPN VYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIA YDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITT RVNSEQEHFLIVPFGLLYSEVTASSLVKINLQG DIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCV VHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYH DYHGILVDEEEKVLIQKNLGPKSKVLILRNHGL VSVGESVEEAFYYIHNLVVACEIQVRTLASAGG PDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQ IGEQEFEALMRMLDNLGYRTGYPYRYPALREKS KKYSDVEVPASVTGYSFASDGDSGTCSPLRHSF QKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTK VWTNITHDHVKPLLQSLSSGVCVPSCITNCLWT KEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKI REQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVT ASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELE EYRREVERKQKGSEENLDEAREQKEKSPPDQPA VPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPT LPDLSPDEPSEALGFPMLEKEEEAHRPPSPTEA PTEASPEPAPDPAPVAEEAAPSAVEEGAAADPG SDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS*KP CANTVLSGATLALPASPATSVLCPCVMECKKAK PSA*RSPHVTSPV*PRADPVCAQQPHPTLPLVL SEPQLLGETCSPHRGEALSHGPGWKVLKASAALA ARHPPEPHLSCRPSCCEITTFCLCLASPPP*SL R*RTQTPGFMWHSLCSVISLKSIYKALVP*KHS CAHQRP*RGRSGPDAGQETPAADGPVCTQRDAMH V*PTIPRPESDSGAQFLFHLGLWIPVIGKVYQE GKRPF*NFL*AGLQFSLHFSTVNVNRLLFAFFF PDPTVEHFSLIWGRLQGTVLTLTQPERCPSCDQR *LEVEGSPGAPRHHRH*WSLNRVLLGRCCCCYF PPRGWKRAQSC*AGTGG*PISGGAGTSLTRYRQ ACVGPTGPW*SWCQMWAQILGMMGRATSDPTDWP AEREPGCCMALTAASSFL*AAGPATRASVLPLF CVVLLPRQNEKSLTVDSSFIPSFTPHMQ*LLIF TFVV*SFVLQHEI*LHIWTPTWEDRS*MSFLQCN MFYSQIKFFQQ

Sequence NM_176801

GCACCCAGGTCGGGCGGTGGGGGCGAGCGGAGGGGCTGAGGGGCGGAGAGGCCTGGCGGGCCGCTGCTGCGGGCCAGGGGACGGGGGCGGAGCCGGAGCC GGAGCCGACGGGCGGTGGCCGCACTGGGACCCCGGAATCCCGCGCGCTGCCCACGATTCGCTTCTGAGGAACCTAGAAAGATTGTACAATGAATGGTGAT TCTCGTGCTGCGGTGGTGACCTCACCACCCCCGACCACAGCCCCTCACAAGGAGAGGTACTTCGACCGAGTAGATGAGAACAACCCAGAGTACTTGAGGG AGAGGAACATGGCACCAGACCTTCGCCAGGACTTCAACATGATGGAGCAAAAGAAGAGGGTGTCCATGATTCTGCAAAGCCCTGCTTTCTGTGAAGAATT GGAATCAATGATACAGGAGCAATTTAAGAAGGGGAAGAACCCCACAGGCCTATTGGCATTACAGCAGATTGCAGATTTTATGACCACGAATGTACCAAAT GTCTACCCAGCAGCTCCGCAAGGAGGGATGGCTGCCTTAAACATGAGTCTTGGTATGGTGACTCCTGTGAACGATCTTAGAGGATCTGATTCTATTGCGT ATGACAAAGGAGAGAAGTTATTACGGTGTAAATTGGCAGCGTTTTATAGACTAGCAGATCTCTTTGGGTGGTCTCAGCTTATCTACAATCATATCACAAC CAGAGTGAACTCCGAGCAGGAACACTTCCTCATTGTCCCTTTTGGGCTTCTTTACAGTGAAGTGACTGCATCCAGTTTGGTTAAGATCAATCTACAAGGA GATATAGTAGATCGTGGAAGCACTAATCTGGGAGTGAATCAGGCCGGCTTCACCTTACACTCTGCAATTTATGCTGCACGCCCGGACGTGAAGTGCGTCG TGCACATTCACACCCCAGCAGGGGCTGCGGTCTCTGCAATGAAATGTGGCCTCTTGCCAATCTCCCCGGAGGCGCTTTCCCTTGGAGAAGTGGCTTATCA TGACTACCATGGCATTCTGGTTGATGAAGAGGAAAAAGTTTTGATTCAGAAAAATCTGGGGCCTAAAAGCAAGGTTCTTATTCTCCGGAACCATGGGCTC GTGTCAGTTGGAGAGAGCGTTGAGGAGGCCTTCTATTACATCCATAACCTTGTGGTTGCCTGTGAGATCCAGGTTCGAACTCTGGCCAGTGCAGGAGGAC CAGACAACTTAGTCCTGCTGAATCCTGAGAAGTACAAAGCCAAGTCCCGTTCCCCAGGGTCTCCGGTAGGGGAAGGCACTGGATCGCCTCCCAAGTGGCA GATTGGTGAGCAGGAATTTGAAGCCCTCATGCGGATGCTCGATAATCTGGGCTACAGAACTGGCTACCCTTATCGATACCCTGCTCTGAGAGAGAAGTCT AAAAAATACAGCGATGTGGAGGTTCCTGCTAGTGTCACAGGTTACTCCTTTGCTAGTGACGGTGATTCGGGCACTTGCTCCCCACTCAGACACAGTTTTC AGAAGCAGCAGCGGGAGAAGACAAGATGGCTGAACTCTGGCCGGGGCGACGAAGCTTCCGAGGAAGGGCAGAATGGAAGCAGTCCCAAGTCGAAGACTAA GGTGTGGACGAACATTACACACGATCACGTGAAACCCTTGCTGCAGTCTCTCTCGTCCGGTGTCTGCGTGCCAAGCTGTATTACCAACTGCTTGTGGACT AAAGAGGATGGACATAGAACTTCCACCTCTGCTGTCCCTAACCTGTTTGTTCCATTGAACACTAACCCAAAAGAGGTCCAGGAGATGAGGAACAAGATCC GAGAGCAGAATTTACAGGACATTAAGACGGCTGGCCCTCAGTCCCAGGTTTTGTGTGGTGTAGTGATGGACAGGAGCCTCGTCCAGGGAGAGCTGGTGAC GGCCTCCAAGGCCATCATTGAAAAGGAGTACCAGCCCCACGTCATTGTGAGCACCACGGGCCCCAACCCCTTCACCACACTCACAGACCGTGAGCTGGAG GAGTACCGCAGGGAGGTGGAGAGGAAGCAGAAGGGCTCTGAAGAGAATCTGGACGAGGCTAGAGAACAGAAAGAAAAGAGTCCTCCAGACCAGCCTGCGG TCCCCCACCCGCCTCCCAGCACTCCCATCAAGCTGGAGGAAGGAGACGGATGCGCTAGAGAGTACCTGTTACCCTAACCTTGTGCCGGAGCCGACTACTG GAGATGACAGTGATGCTGCCACCTTTAAGCCAACTCTCCCCGATCTGTCCCCTGATGAACCTTCAGAAGCACTCGGCTTCCCAATGTTAGAGAAGGAGGA GGAAGCCCATAGACCCCCAAGCCCCACTGAGGCCCCTACTGAGGCCAGCCCCGAGCCAGCCCCAGACCCAGCCCCGGTGGCTGAAGAGGCTGCCCCCTCA GCTGTCGAGGAGGGGGCCGCCGCGGACCCTGGCAGCGATGGGTCTCCAGGCAAGTCCCCGTCCAAAAAGAAGAAGAAGTTCCGTACCCCGTCCTTTCTGA AGAAGAGCAAGAAGAAGAGTGACTCCTGAAAGCCCTGCGCTAACACTGTCCTGTCCGGAGCGACCCTGGCTCTGCCAGCGTCCCCGGCCACGTCTGTGCT CTGTCCTTGTGTAATGGAATGCAAAAAAGCCAAGCCCTCCGCCTAGAGGTCCCCTCACGTGACCAGCCCCGTGTAGCCCCGGGCTGACCCAGTGTGTGCT CAGCAGCCCCACCCCACCCTGCCCCTTGTCCTCTCAGAGCCTCAGCTTCTGGGGGAGACATGCTCTCCCCACAGGGGGGAGGCACTAAGTCATGGTCCTG GCTGGAAGGTACTGAAGGCTTCTGCAGCTTTGGCTGCACGTCACCCTCCTGAGCCTCACCTTTCCTGCCGTCCCTCCTGTTGTGAAATCACCACATTCTG TCTCTGCTTGGCTTCCCCTCCACCCTAAAGTCTCAGGTGACGGACTCAGACTCCTGGCTTCATGTGGCATTCTCTCTGCTCAGTGATCTCACTTAAATCT ATATACAAAGCCTTGGTCCCGTGAAAACACTCGTGTGCCCACCAGCGGCCTTGAAGAGGCAGGTCTGGGCCAGATGCTGGGCAGGAAACCCCAGCGGCAG ATGGGCCTGTGTGCACCCAACGTGATGCTATGCATGTCTGACCGACGATCCCTCGACCAGAATCAGATTCAGGAGCTCAGTTTCTTTTTCACTTGGGTCT CTGGATTCCTGTCATAGGGAAGGTATATCAGGAGGGGAAGAGGCCTTTCTAGAATTTTCTTTGAGCAGGTTTACAATTTAGCTTACATTTTTCGACTGTG AACGTGAATAGGCTGCTTTTTGCTTTCTTCTTTCCAGACCCCACAGTAGAGCACTTTTCACTTATTTGGGGGAGGCTTCAGGGGACTGTTCTCACCTTAA CTCAGCCAGAAAGATGCCCTAGTTGTGATCAAAGGTAACTCGAGGTGGAGGGTAGCCCTGGGGCCCCTCGACATCACCGTCATTGATGGAGCCTGAACCG TGTGCTCCTCGGCAGATGCTGTTGTTGTTACTTCCCTCCAAGAGGCTGGAAAAGGGCTCAGAGCTGCTGAGCAGGAACCGGAGGGTGACCCATTTCAGGA GGTGCCGGTACCAGCCTGACTAGGTACAGGCAAGCTTGTGTGGGCCCAACAGGCCCTTGGTAGAGCTGGTGCCAGATGTGGGCTCAGATCCTGGGCATGA TGGGCCGAGCCACCTCGGATCCCACTGATTGGCCAGCCGAGCGAGAACCAGGCTGCTGCATGGCACTGACCGCCGCTTCCAGCTTCCTCTGAGCCGCAGG GCCTGCTACGCGGGCAAGCGTGCTGCCTCTCTTCTGTGTCGTTTTGTTGCCAAGGCAGAATGAAAAGTCCTTAACCGTGGACTCTTCCTTTATCCCCTCC TTTACCCCACATATGCAATGACTTTTAATTTTCACTTTTGTAGTTTAATCCTTTGTATTACAACATGAAATATAGTTGCATATATGGACACCGACTTGGG AGGACAGGTCCTGAATGTCCTTTCTCCAGTGTAACATGTTTTACTCACAAATAAAATTCTTTCAGCAA

refseq_ADD1.F1 (forward primer)
refseq_ADD1.R1 (reverse primer)
Alternative donor

Frame 0

APRSGGGGERRG*GAERPGGPLLRARGRGRSRS RSRRAVAALGPRNPARCPRFASEEPRKIVQ*MVI LVLRW*PHHPRPQPLTRRGTSTE*MRTTQST*G RGTWHQTFARTST*WSKRRGCP*FCKALLSVKN WNQ*YRSNLRRGRTPQAYWHYSRLQIL*PRMYQM STQQLRKEGWLP*T*VLVW*LL*TILEDLILLR MTKERSYYGVNWQRFID*QISLGGLSLSTIISQ PE*TPSRNTSSLSLLGFFTVK*LHPVWLRSIYKE I**IVEALIWE*IRPASPYTLQFMLHART*SAS CTFTPQQGLRSLQ*NVASCQSPRRRFPLEKWLI MTTMAFWLMKRKKF*FRKIWGLKARFLFSGTMGS CQLERALRRPSITSITLWLPVRSRFELWPVQED QTT*SC*ILRSTKPSPVPQGLR*GKALDRLPSG RLVSRNLKPSCGCSIIWATELATLIDTLL*ERSL KNTAMWRFLLVSQVTPLLVTVIRALAPHSDTVF RSSSGRRQDG*TLAGATKLPRKGRMEAVPSRRL RCGRTLHTIT*NPCCSLSRPVSACQAVLPTACGL KRMDIELPPLLSLTCLFH*TLTQKRSRR*GTRS ESRIYRTLRRLALSPRFCVV**WTGASSRESW* RPPRPSLKRSTSPTSL*APRAPTPSPHSQTVSWR STAGRWRGSRRALKRIWTRLENRKKRVLQTSLR SPTRLPALPSSWRKETDALESTCYPNLVPEPTT GDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEE EAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPS AVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFL KKSKKKSDS*KPCANTVLSGATLALPASPATSVL CPCVMECKKAKPSA*RSPHVTSPV*PRADPVCA QQPHPTLPLVLSEPQLLGETCSPHRGEALSHGP GWKVLKASAALAARHPPEPHLSCRPSCCEITTFC LCLASPPP*SLR*RTQTPGFMWHSLCSVISLKS IYKALVP*KHSCAHQRP*RGRSGPDAGQETPAA DGPVCTQRDAMHV*PTIPRPESDSGAQFLFHLGL WIPVIGKVYQEGKRPF*NFL*AGLQFSLHFSTV NVNRLLFAFFFPDPTVEHFSLIWGRLQGTVLTL TQPERCPSCDQR*LEVEGSPGAPRHHRH*WSLNR VLLGRCCCCYFPPRGWKRAQSC*AGTGG*PISG GAGTSLTRYRQACVGPTGPW*SWCQMWAQILGM MGRATSDPTDWPAEREPGCCMALTAASSFL*AAG PATRASVLPLFCVVLLPRQNEKSLTVDSSFIPS FTPHMQ*LLIFTFVV*SFVLQHEI*LHIWTPTW EDRS*MSFLQCNMFYSQIKFFQQ

Frame 1

HPGRAVGASGGAEGRRGLAGRCCGPGDGGGAGA GADGRWPHWDPGIPRAAHDSLLRNLERLYNEW* FSCCGGDLTTPDHSPSQGEVLRPSR*EQPRVLEG EEHGTRPSPGLQHDGAKEEGVHDSAKPCFL*RI GINDTGAI*EGEEPHRPIGITADCRFYDHECTK CLPSSSARRDGCLKHESWYGDSCERS*RI*FYCV *QRREVITV*IGSVL*TSRSLWVVSAYLQSYHN QSELRAGTLPHCPFWASLQ*SDCIQFG*DQSTR RYSRSWKH*SGSESGRLHLTLCNLCCTPGREVRR AHSHPSRGCGLCNEMWPLANLPGGAFPWRSGLS *LPWHSG**RGKSFDSEKSGA*KQGSYSPEPWA RVSWRER*GGLLLHP*PCGCL*DPGSNSGQCRRT RQLSPAES*EVQSQVPFPRVSGRGRHWIASQVA DW*AGI*SPHADAR*SGLQNWLPLSIPCSEREV *KIQRCGGSC*CHRLLLC**R*FGHLLPTQTQFS EAAAGEDKMAELWPGRRSFRGRAEWKQSQVED* GVDEHYTRSRETLAAVSLVRCLRAKLYYQLLVD *RGWT*NFHLCCP*PVCSIEH*PKRGPGDEEQDP RAEFTGH*DGWPSVPGFVWCSDGQEPRPGRAGD GLQGHH*KGVPAPRHCEHHGPQPLHHTHRP*AG GVPQGGGEEAEGL*RESGRG*RTERKESSRPACG PPPASQHSHQAGGRRRMR*RVPVTLTLCRSRLL EMTVMLPPLSQLSPICPLMNLQKHSASQC*RRR RKPIDPQAPLRPLLRPAPSQPQTQPRWLKRLPPQ LSRRGPPRTLAAMGLQASPRPKRRRSSVPRPF* RRARRRVTPESPALTLSCPERPWLCQRPRPRLC SVLV*WNAKKPSPPPRGPLT*PAPCSPGLTQCVL SSPTPPCPLSSQSLSFWGRHALPTGGRH*VMVL AGRY*RLLQLWLHVTLLSLTFPAVPPVVKSPHS VSAWLPLHPKVSGDGLRLLASCGILSAQ*SHLNL YTKPWSRENTRVPTSGLEEAGLGQMLGRKPQRQ MGLCAPNVMLCMSDRRSLDQNQIQELSFFFTWV SGFLS*GRYIRRGRGLSRIFFEQVYNLAYIFRL* T*IGCFLLSSFQTPQ*STFHLFGGGFRGLFSP* LSQKDALVVIKGNSRWRVALGPLDITVIDGA*T VCSSADAVVVTSLQEAGKGLRAAEQEPEGDPFQE VPVPA*LGTGKLVWAQQALGRAGARCGLRSWA* WAEPPRIPLIGQPSENQAAAWH*PPLPASSEPQ GLLRGQACCLSSVSFCCQGRMKSP*PWTLPLSPP LPHICNDF*FSLL*FNPLYYNMKYSCIYGHRLG RTGPECPFSSVTCFTHK*NSFS

Frame 2

TQVGRWGRAEGLRGGEAWRAAAAGQGTGAEPEP EPTGGGRTGTPESRALPTIRF*GT*KDCTMNGD SRAAVVTSPPPTTAPHKERYFDRVDENNPEYLR ERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEEL ESMIQEQFKKGKNPTGLLALQQIADFMTTNVPN VYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIA YDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITT RVNSEQEHFLIVPFGLLYSEVTASSLVKINLQG DIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCV VHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYH DYHGILVDEEEKVLIQKNLGPKSKVLILRNHGL VSVGESVEEAFYYIHNLVVACEIQVRTLASAGG PDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQ IGEQEFEALMRMLDNLGYRTGYPYRYPALREKS KKYSDVEVPASVTGYSFASDGDSGTCSPLRHSF QKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTK VWTNITHDHVKPLLQSLSSGVCVPSCITNCLWT KEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKI REQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVT ASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELE EYRREVERKQKGSEENLDEAREQKEKSPPDQPA VPHPPPSTPIKLEEGDGCAREYLLP*PCAGADYW R*Q*CCHL*ANSPRSVP**TFRSTRLPNVREGG GSP*TPKPH*GPY*GQPRASPRPSPGG*RGCPL SCRGGGRRGPWQRWVSRQVPVQKEEEVPYPVLSE EEQEEE*LLKALR*HCPVRSDPGSASVPGHVCA LSLCNGMQKSQALRLEVPSRDQPRVAPG*PSVC SAAPPHPAPCPLRASASGGDMLSPQGGGTKSWSW LEGTEGFCSFGCTSPS*ASPFLPSLLL*NHHIL SLLGFPSTLKSQVTDSDSWLHVAFSLLSDLT*I YIQSLGPVKTLVCPPAALKRQVWARCWAGNPSGR WACVHPT*CYACLTDDPSTRIRFRSSVSFSLGS LDSCHREGISGGEEAFLEFSLSRFTI*LTFFDC ERE*AAFCFLLSRPHSRALFTYLGEASGDCSHLN SARKMP*L*SKVTRGGG*PWGPSTSPSLMEPEP CAPRQMLLLLLPSKRLEKGSELLSRNRRVTHFR RCRYQPD*VQASLCGPNRPLVELVPDVGSDPGHD GPSHLGSH*LASRARTRLLHGTDRRFQLPLSRR ACYAGKRAASLLCRFVAKAE*KVLNRGLFLYPL LYPTYAMTFNFHFCSLILCITT*NIVAYMDTDLG GQVLNVLSPV*HVLLTNKILSA