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Sequences

Sequence NM_198977

AGAGCCAGGAAGCGGGAGCCGGGACCCAGGGCCCGGGATCGCCGAGCCCGACCTCGGGCGCCCCGCCGGTCACCTCCGCGCGGACACCAGCCCTGCAGAG CCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATT TTGAGAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGCCCAGCCCACCTCATGGCCCTCCTGCA GCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACTAGGGCTGACCTCATC TCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGG GCATGACGCCCTGGGAGCAGGAGCTGGCCCAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAGCGGCT GCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGGTCAACGCCATTGGCCTGTACATGCGCCACCTTGGG GTGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAGACCAAGAAGG GGCTGAGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAGCAGAGGTTGATGCCGAGAAGCCAGG TGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAATATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCC CTGGACAGCCCAGACCGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGTCCTTGGCGCCCCCAG AGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGAGCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGG CTGGCGGGAACTCGTCCCCCCAGACACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGGCGGCC CACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCCTTGGAGGAGCTGCAGAACATCTTCCCCAGCC TGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTCGATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCT GGCCCGGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGCCTTAGAGCAGCTCAAAGCCAAGCAA CGCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGCCGCCCGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACGGAGATGCAGC GGCTGACCAAGTACCCCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGCCGAGTGCTGCCGGGA AATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTCAAGGACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGC AGCGACCCTATGCTGAGCGAGTTCAAGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAGGACAAGGCAG TGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTGCTGCTCAAGTCCCATAGCCGGACACTGACGCCCAC GCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGCGGCTCACCTCCGCCATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACC TGGGACCAGGAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATCACTGAGACTGCCGGATCCCTGAAAG TCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCAGCACCCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGAC GTCTCCAGCTGATGCCCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCCACGGCCCTTCGGAAA GTGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGCCTCCTCGAGATGGGGATGGGGTCCCAGGGGGCGGCCCCCTGAGCC CAGCACGGACCCAGGAAATCCAGGAGAACCTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGACCCCT CCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACTTGAGGTTCCCGCCCAGGAAGGCCTTTTGCAAGAAGGAGAGGAATGGGGGAGAGG ACGTGAGGGACCACCCCCACCCACACAGCTGCCGCAGCATCTCACACCCCGAGGGCCTGAGGAGAGGGAGCTGTGGGCCACGCCTGGGAGGGGCCCAGCT GGGGTTACTGGCCCCGCATGAGCCTCGGCCATCTCTCCCTCCTGCCCTCTGCTTGGGGGACTCAGGGCTCCATTCTGGAGGGCACCACGGTGACCCGGGC CATCTCAGTATTGCCTGTGGGGGCCACCCCTCCACCCCCACCCCCAAGTGCCTTCGCTCTGTTTTTATACCCTGAATTGGAGGTTTATTTTTTAATATAT ATTATCTAAGAAGA

refseq_ARHGEF1.F2 (forward primer)
refseq_ARHGEF1.R2 (reverse primer)
Single exon skipping

Frame 0

RARKREPGPRARDRRARPRAPRRSPPRGHQPCR AQGDGRLRPRGGLPRPLPAWPGSRQHHRG*G*GF *ERAGDKLRRAKQPVPEPGAGEAAPSPPHGPPA ARGPAV*ARTPGSPGAGPSQRRL*T*PH*G*PH LRGCPAAVRAGGGAKPAGSRGPAAGGLPFQAAHG HDALGAGAGPAGGLGWAGPSQLRGPGAARGGAA AHAPGGDATYHLYRRRKECCRGQRHWPVHAPPW GADQEWRQEVGEELLPEKGDGEPAVGRACQDQEG AEQHPGCRPLEPGRAPGSRFSTPQSRG*CREAR CYRPEGRRGDALSGPEYRGSWAGHPWSLSAPSV PGQPRPGTRC*RPPGAGGLIPAGPNEPGVLGAPR EYRRGGRNREPRAWR*GGAGAVGTGA*TRRASR LAGTRPPRHPAQPAQEPGEAAGGHQRAAGDRGG PRAHAAGAARPLLPAHGRMPVLPLGGAAEHLPQP GRAHRGAFPVPRSPDEAEAGEWLPHRGDRRRAA GPV*WC*GLLVPENLLPLLQPPVICLRAAQSQA TQGPSVLCLRAGS*EPPAVPPPAAEGHDPHGDAA ADQVPPAPAEHRAEHRRAHRTGESGAGSRVLPG NSTPRQPSRA*HGGPAEAQGLSAAPGLVPPSAE QRPYAERVQEPGHHQEEIGPRGPTDVAGD*GQGS GGACAAAGRPAAAAPAPGRAAAAQVP*PDTDAH ARWQDHAAARAAAHLRHDPRGGHRSQSLLRPFY LGPGGPDIRAGGTDCVGAEKLVCSHH*DCRIPES PCPCLSP*APAQPEQHPRTPPQQL*ERQWWPRD VSS*CPDRENPQ*PPALLQTRPRGPARCHGPSE SAVPEAASVSGGGRQWGGASSRWGWGPRGRPPEP STDPGNPGEPAQLGGDHEAAGGVGGGILPPETP PVSAWGELCPPAWLHLRFPPRKAFCKKERNGGE DVRDHPHPHSCRSISHPEGLRRGSCGPRLGGAQL GLLAPHEPRPSLPPALCLGDSGLHSGGHHGDPG HLSIACGGHPSTPTPKCLRSVFIP*IGGLFFNI YYLRR

Frame 1

EPGSGSRDPGPGIAEPDLGRPAGHLRADTSPAE PREMEDFARGAASPGPSRPGLVPVSIIGAEDED FENELETNSEEQNSQFQSLEQVKRRPAHLMALLQ HVALQFEPGPLVLRVPVPPNVAFELDRTRADLI SEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLM GMTPWEQELAQLEAWVGRDRASYEARERHVAERL LMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLG VRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKK GLSSILDAARWNRGEPQVPDFRHLKAEVDAEKPG ATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLS LDSPDREPGADAPLELGDSSPQGPMSLESLAPP ESTDEGAETESPEPGDEGEPGRSGLELEPEEPPG WRELVPPDTLHSLPKSQVKRQEVISELLVTEAA HVRMLRVLHDLFFQPMAECLFFPLEELQNIFPS LDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLL ARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQ RKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQ RLTKYPLLLQSIGQNTEEPTEREKVELAAECCRE ILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQS SDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKA VEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPT PDGKTMLRPVLRLTSAMTREVATDHKAFYVLFT WDQEAQIYELVAQTVSERKNWCALITETAGSLK VPAPASRPKPRPSPSSTREPLLSSSENGNGGRET SPADARTERILSDLLPFCRPGPEGQLAATALRK VLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLS PARTQEIQENLLSLEETMKQLEELEEEFCRLRPL LSQLGGNSVPQPGCT*GSRPGRPFARRRGMGER T*GTTPTHTAAAASHTPRA*GEGAVGHAWEGPS WGYWPRMSLGHLSLLPSAWGTQGSILEGTTVTRA ISVLPVGATPPPPPPSAFALFLYPELEVYFLIY II*E

Frame 2

SQEAGAGTQGPGSPSPTSGAPPVTSARTPALQS PGRWKTSPEGRPPQAPPGLAWFPSASSGLRMRI LRTSWRQTQKSKTASSRAWSR*SGAQPTSWPSC STWPCSLSQDPWFSGCRSLPTSPLNLTALGLTSS PRMSSGGSCRRWCKASR*PWAGSWRTSVPSGSW A*RPGSRSWPSWRLGLGGTEPATRPGSGTWRSG CSCTWRRCNIPSLPTKKRVLPWSTPLACTCATLG CGPRVETRSRGGTSSGKR*WGTGGRTSLPRPRR G*AASWMPPAGTGESPRFQIFDTSKQRLMPRSQ VLQTGREAWGCPLGTGISGLLGRTPLESLCTLCP WTAQTGNQVLTPPWSWGTHPRRAQ*AWSPWRPQ RVPTRGPKPRAPSLEMRGSRGGRDWSLNQKSLP AGGNSSPQTPCTACPRAR*SGRRSSASCW*QRRP TCACCGCCTTSSSSPWQNACSSPWRSCRTSSPA WTSSSRCIPCSSIA**SGGRRVATSSRRSETCC WPGLMVLRAPGSRKSPPASAAASHLP*SSSKPSN ARTLGSVPSCRKLRAARGAAACS*RT*SPRRCS G*PSTPCSCRASGRTQKSPQNGRKWSWQPSAAG KFYTTSTKPCVTWRTC*GSRTISGAWTCPTFGRA ATLC*ASSRTWTSPRRNWSTRAH*RGG*LRTRQ WRCMCCCWTTCCCCSSARTSGCCSSPIAGH*RP RPMARPCCGPCCGSPPP*PARWPPITKPSTSFLP GTRRPRYTSWWHRLCRSGKTGVLSSLRLPDP*K SLPLPLALSPGPARAAPENPSSAALRTAMVAER RLQLMPGPRESSVTSCPSADQAPRASSLPRPFGK CCP*SSFCFRRRKTMGRGLLEMGMGSQGAAP*A QHGPRKSRRTCSAWRRP*SSWRSWRRNFAA*DP SCLSLGGTLSPSLAALEVPAQEGLLQEGEEWGRG REGPPPPTQLPQHLTPRGPEERELWATPGRGPA GVTGPA*ASAISPSCPLLGGLRAPFWRAPR*PG PSQYCLWGPPLHPHPQVPSLCFYTLNWRFIF*YI LSKK

Sequence NM_004706

AGAGCCAGGAAGCGGGAGCCGGGACCCAGGGCCCGGGATCGCCGAGCCCGACCTCGGGCGCCCCGCCGGTCACCTCCGCGCGGACACCAGCCCTGCAGAG CCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGCATCATCGGGGCTGAGGATGAGGATT TTGAGAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGCCCAGCCCACCTCATGGCCCTCCTGCA GCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGCTTTGCTGTCTGCATGCCGACATGCTGGGCTCACTGGGCCCCAAGGAGGCCAAGAAGGCCTTCCTG GACTTCTACCACAGCTTCCTGGAGAAGACAGCGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTTGACCGCACTAGGGCTGACCTCATCT CCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGGCAGCTGGAGGACTTCCGTTCCAAGCGGCTCATGGG CATGACGCCCTGGGAGCAGGAGCTGGCCCAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCGGGAGCGGCACGTGGCGGAGCGGCTG CTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGGTCAACGCCATTGGCCTGTACATGCGCCACCTTGGGG TGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGGACGAGCCTGCCAAGACCAAGAAGGG GCTGAGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAGCAGAGGTTGATGCCGAGAAGCCAGGT GCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAATATCGGGGCTCCTGGGCAGGACACCCCTGGAGTCTCTCTGCACCCTCTGTCCC TGGACAGCCCAGACCGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGAGCCTGGAGTCCTTGGCGCCCCCAGA GAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGAGCCGGGGCGGTCGGGACTGGAGCTTGAACCAGAAGAGCCTCCCGGC TGGCGGGAACTCGTCCCCCCAGACACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGCGAGCTGCTGGTGACAGAGGCGGCCC ACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCCTTGGAGGAGCTGCAGAACATCTTCCCCAGCCT GGACGAGCTCATCGAGGTGCATTCCCTGTTCCTCGATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCATCGAGGAGATCGGAGACGTGCTGCTG GCCCGGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGCCTTAGAGCAGCTCAAAGCCAAGCAAC GCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGCCGCCCGCGGTGCCGCCGCCTGCAGCTGAAGGACATGATCCCCACGGAGATGCAGCG GCTGACCAAGTACCCCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGAGCTGGCAGCCGAGTGCTGCCGGGAA ATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTCAAGGACTATCAGCGGCGCCTGGACTTGTCCCACCTTCGGCAGAGCA GCGACCCTATGCTGAGCGAGTTCAAGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGTGGCGGGTGACTAAGGACAAGGCAGT GGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTGCTGCTCAAGTCCCATAGCCGGACACTGACGCCCACG CCCGATGGCAAGACCATGCTGCGGCCCGTGCTGCGGCTCACCTCCGCCATGACCCGCGAGGTGGCCACCGATCACAAAGCCTTCTACGTCCTTTTTACCT GGGACCAGGAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATCACTGAGACTGCCGGATCCCTGAAAGT CCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCAGCACCCGAGAACCCCTCCTCAGCAGCTCTGAGAACGGCAATGGTGGCCGAGAGACG TCTCCAGCTGATGCCCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAGCTCGCTGCCACGGCCCTTCGGAAAG TGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGCCTCCTCGAGATGGGGATGGGGTCCCAGGGGGCGGCCCCCTGAGCCC AGCACGGACCCAGGAAATCCAGGAGAACCTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGAGGAATTTTGCCGCCTGAGACCCCTC CTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACTTGAGGTTCCCGCCCAGGAAGGCCTTTTGCAAGAAGGAGAGGAATGGGGGAGAGGA CGTGAGGGACCACCCCCACCCACACAGCTGCCGCAGCATCTCACACCCCGAGGGCCTGAGGAGAGGGAGCTGTGGGCCACGCCTGGGAGGGGCCCAGCTG GGGTTACTGGCCCCGCATGAGCCTCGGCCATCTCTCCCTCCTGCCCTCTGCTTGGGGGACTCAGGGCTCCATTCTGGAGGGCACCACGGTGACCCGGGCC ATCTCAGTATTGCCTGTGGGGGCCACCCCTCCACCCCCACCCCCAAGTGCCTTCGCTCTGTTTTTATACCCTGAATTGGAGGTTTATTTTTTAATATATA TTATCTAAGAAGA

refseq_ARHGEF1.F2 (forward primer)
refseq_ARHGEF1.R2 (reverse primer)
Single exon skipping

Frame 0

RARKREPGPRARDRRARPRAPRRSPPRGHQPCR AQGDGRLRPRGGLPRPLPAWPGSRQHHRG*G*GF *ERAGDKLRRAKQPVPEPGAGEAAPSPPHGPPA ARGPAV*ARTPALLSACRHAGLTGPQGGQEGLP GLLPQLPGEDSGSPGAGPSQRRL*T*PH*G*PHL RGCPAAVRAGGGAKPAGSRGPAAGGLPFQAAHG HDALGAGAGPAGGLGWAGPSQLRGPGAARGGAA AHAPGGDATYHLYRRRKECCRGQRHWPVHAPPWG ADQEWRQEVGEELLPEKGDGEPAVGRACQDQEG AEQHPGCRPLEPGRAPGSRFSTPQSRG*CREAR CYRPEGRRGDALSGPEYRGSWAGHPWSLSAPSVP GQPRPGTRC*RPPGAGGLIPAGPNEPGVLGAPR EYRRGGRNREPRAWR*GGAGAVGTGA*TRRASR LAGTRPPRHPAQPAQEPGEAAGGHQRAAGDRGGP RAHAAGAARPLLPAHGRMPVLPLGGAAEHLPQP GRAHRGAFPVPRSPDEAEAGEWLPHRGDRRRAA GPV*WC*GLLVPENLLPLLQPPVICLRAAQSQAT QGPSVLCLRAGS*EPPAVPPPAAEGHDPHGDAA ADQVPPAPAEHRAEHRRAHRTGESGAGSRVLPG NSTPRQPSRA*HGGPAEAQGLSAAPGLVPPSAEQ RPYAERVQEPGHHQEEIGPRGPTDVAGD*GQGS GGACAAAGRPAAAAPAPGRAAAAQVP*PDTDAH ARWQDHAAARAAAHLRHDPRGGHRSQSLLRPFYL GPGGPDIRAGGTDCVGAEKLVCSHH*DCRIPES PCPCLSP*APAQPEQHPRTPPQQL*ERQWWPRD VSS*CPDRENPQ*PPALLQTRPRGPARCHGPSES AVPEAASVSGGGRQWGGASSRWGWGPRGRPPEP STDPGNPGEPAQLGGDHEAAGGVGGGILPPETP PVSAWGELCPPAWLHLRFPPRKAFCKKERNGGED VRDHPHPHSCRSISHPEGLRRGSCGPRLGGAQL GLLAPHEPRPSLPPALCLGDSGLHSGGHHGDPG HLSIACGGHPSTPTPKCLRSVFIP*IGGLFFNIY YLRR

Frame 1

EPGSGSRDPGPGIAEPDLGRPAGHLRADTSPAE PREMEDFARGAASPGPSRPGLVPVSIIGAEDED FENELETNSEEQNSQFQSLEQVKRRPAHLMALLQ HVALQFEPGPLLCCLHADMLGSLGPKEAKKAFL DFYHSFLEKTAVLRVPVPPNVAFELDRTRADLI SEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMG MTPWEQELAQLEAWVGRDRASYEARERHVAERL LMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLG VRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKG LSSILDAARWNRGEPQVPDFRHLKAEVDAEKPG ATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLS LDSPDREPGADAPLELGDSSPQGPMSLESLAPPE STDEGAETESPEPGDEGEPGRSGLELEPEEPPG WRELVPPDTLHSLPKSQVKRQEVISELLVTEAA HVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSL DELIEVHSLFLDRLMKRRQESGYLIEEIGDVLL ARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQ RKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQR LTKYPLLLQSIGQNTEEPTEREKVELAAECCRE ILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQS SDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAV EVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPT PDGKTMLRPVLRLTSAMTREVATDHKAFYVLFT WDQEAQIYELVAQTVSERKNWCALITETAGSLKV PAPASRPKPRPSPSSTREPLLSSSENGNGGRET SPADARTERILSDLLPFCRPGPEGQLAATALRK VLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSP ARTQEIQENLLSLEETMKQLEELEEEFCRLRPL LSQLGGNSVPQPGCT*GSRPGRPFARRRGMGER T*GTTPTHTAAAASHTPRA*GEGAVGHAWEGPSW GYWPRMSLGHLSLLPSAWGTQGSILEGTTVTRA ISVLPVGATPPPPPPSAFALFLYPELEVYFLIY II*E

Frame 2

SQEAGAGTQGPGSPSPTSGAPPVTSARTPALQS PGRWKTSPEGRPPQAPPGLAWFPSASSGLRMRI LRTSWRQTQKSKTASSRAWSR*SGAQPTSWPSC STWPCSLSQDPCFAVCMPTCWAHWAPRRPRRPSW TSTTASWRRQRFSGCRSLPTSPLNLTALGLTSS PRMSSGGSCRRWCKASR*PWAGSWRTSVPSGSW A*RPGSRSWPSWRLGLGGTEPATRPGSGTWRSGC SCTWRRCNIPSLPTKKRVLPWSTPLACTCATLG CGPRVETRSRGGTSSGKR*WGTGGRTSLPRPRR G*AASWMPPAGTGESPRFQIFDTSKQRLMPRSQV LQTGREAWGCPLGTGISGLLGRTPLESLCTLCP WTAQTGNQVLTPPWSWGTHPRRAQ*AWSPWRPQ RVPTRGPKPRAPSLEMRGSRGGRDWSLNQKSLPA GGNSSPQTPCTACPRAR*SGRRSSASCW*QRRP TCACCGCCTTSSSSPWQNACSSPWRSCRTSSPA WTSSSRCIPCSSIA**SGGRRVATSSRRSETCCW PGLMVLRAPGSRKSPPASAAASHLP*SSSKPSN ARTLGSVPSCRKLRAARGAAACS*RT*SPRRCS G*PSTPCSCRASGRTQKSPQNGRKWSWQPSAAGK FYTTSTKPCVTWRTC*GSRTISGAWTCPTFGRA ATLC*ASSRTWTSPRRNWSTRAH*RGG*LRTRQ WRCMCCCWTTCCCCSSARTSGCCSSPIAGH*RPR PMARPCCGPCCGSPPP*PARWPPITKPSTSFLP GTRRPRYTSWWHRLCRSGKTGVLSSLRLPDP*K SLPLPLALSPGPARAAPENPSSAALRTAMVAERR LQLMPGPRESSVTSCPSADQAPRASSLPRPFGK CCP*SSFCFRRRKTMGRGLLEMGMGSQGAAP*A QHGPRKSRRTCSAWRRP*SSWRSWRRNFAA*DPS CLSLGGTLSPSLAALEVPAQEGLLQEGEEWGRG REGPPPPTQLPQHLTPRGPEERELWATPGRGPA GVTGPA*ASAISPSCPLLGGLRAPFWRAPR*PGP SQYCLWGPPLHPHPQVPSLCFYTLNWRFIF*YI LSKK

Sequence NM_199002

GGTGGACTCAGGGCGGCTAGAGCGACGCGGCGGCAGGGGTGGGGAGAGTGCGGAGCCCGAGCGCGGAGGCTTCGGTTCCGGTGGCGGCGATGGCTTCTCT TTCCACCTGGAGCAGCCCTGCAGAGCCCAGGGAGATGGAAGACTTCGCCCGAGGGGCGGCCTCCCCAGGCCCCTCCCGGCCTGGCCTGGTTCCCGTCAGC ATCATCGGGGCTGAGGATGAGGATTTTGAGAACGAGCTGGAGACAAACTCAGAAGAGCAAAACAGCCAGTTCCAGAGCCTGGAGCAGGTGAAGCGGCGCC CAGCCCACCTCATGGCCCTCCTGCAGCACGTGGCCCTGCAGTTTGAGCCAGGACCCCTGCTTTGCTGTCTGCATGCCGACATGCTGGGCTCACTGGGCCC CAAGGAGGCCAAGAAGGCCTTCCTGGACTTCTACCACAGCTTCCTGGAGAAGACAGCGGTTCTCCGGGTGCCGGTCCCTCCCAACGTCGCCTTTGAACTT GACCGCACTAGGGCTGACCTCATCTCCGAGGATGTCCAGCGGCGGTTCGTGCAGGAGGTGGTGCAAAGCCAGCAGGTAGCCGTGGGCCGGCAGCTGGAGG ACTTCCGTTCCAAGCGGCTCATGGGCATGACGCCCTGGGAGCAGGAGCTGGCCCAGCTGGAGGCTTGGGTTGGGCGGGACCGAGCCAGCTACGAGGCCCG GGAGCGGCACGTGGCGGAGCGGCTGCTCATGCACCTGGAGGAGATGCAACATACCATCTCTACCGACGAAGAAAAGAGTGCTGCCGTGGTCAACGCCATT GGCCTGTACATGCGCCACCTTGGGGTGCGGACCAAGAGTGGAGACAAGAAGTCGGGGAGGAACTTCTTCCGGAAAAAGGTGATGGGGAACCGGCGGTCGG ACGAGCCTGCCAAGACCAAGAAGGGGCTGAGCAGCATCCTGGATGCCGCCCGCTGGAACCGGGGAGAGCCCCAGGTTCCAGATTTTCGACACCTCAAAGC AGAGGTTGATGCCGAGAAGCCAGGTGCTACAGACCGGAAGGGAGGCGTGGGGATGCCCTCTCGGGACCGGAATATCGGGGCTCCTGGGCAGGACACCCCT GGAGTCTCTCTGCACCCTCTGTCCCTGGACAGCCCAGACCGGGAACCAGGTGCTGACGCCCCCCTGGAGCTGGGGGACTCATCCCCGCAGGGCCCAATGA GCCTGGAGTCCTTGGCGCCCCCAGAGAGTACCGACGAGGGGGCCGAAACCGAGAGCCCCGAGCCTGGAGATGAGGGGGAGCCGGGGCGGTCGGGACTGGA GCTTGAACCAGAAGAGCCTCCCGGCTGGCGGGAACTCGTCCCCCCAGACACCCTGCACAGCCTGCCCAAGAGCCAGGTGAAGCGGCAGGAGGTCATCAGC GAGCTGCTGGTGACAGAGGCGGCCCACGTGCGCATGCTGCGGGTGCTGCACGACCTCTTCTTCCAGCCCATGGCAGAATGCCTGTTCTTCCCCTTGGAGG AGCTGCAGAACATCTTCCCCAGCCTGGACGAGCTCATCGAGGTGCATTCCCTGTTCCTCGATCGCCTGATGAAGCGGAGGCAGGAGAGTGGCTACCTCAT CGAGGAGATCGGAGACGTGCTGCTGGCCCGGTTTGATGGTGCTGAGGGCTCCTGGTTCCAGAAAATCTCCTCCCGCTTCTGCAGCCGCCAGTCATTTGCC TTAGAGCAGCTCAAAGCCAAGCAACGCAAGGACCCTCGGTTCTGTGCCTTCGTGCAGGAAGCTGAGAGCCGCCCGCGGTGCCGCCGCCTGCAGCTGAAGG ACATGATCCCCACGGAGATGCAGCGGCTGACCAAGTACCCCCTGCTCCTGCAGAGCATCGGGCAGAACACAGAAGAGCCCACAGAACGGGAGAAAGTGGA GCTGGCAGCCGAGTGCTGCCGGGAAATTCTACACCACGTCAACCAAGCCGTGCGTGACATGGAGGACCTGCTGAGGCTCAAGGACTATCAGCGGCGCCTG GACTTGTCCCACCTTCGGCAGAGCAGCGACCCTATGCTGAGCGAGTTCAAGAACCTGGACATCACCAAGAAGAAATTGGTCCACGAGGGCCCACTGACGT GGCGGGTGACTAAGGACAAGGCAGTGGAGGTGCATGTGCTGCTGCTGGACGACCTGCTGCTGCTGCTCCAGCGCCAGGACGAGCGGCTGCTGCTCAAGTC CCATAGCCGGACACTGACGCCCACGCCCGATGGCAAGACCATGCTGCGGCCCGTGCTGCGGCTCACCTCCGCCATGACCCGCGAGGTGGCCACCGATCAC AAAGCCTTCTACGTCCTTTTTACCTGGGACCAGGAGGCCCAGATATACGAGCTGGTGGCACAGACTGTGTCGGAGCGGAAAAACTGGTGTGCTCTCATCA CTGAGACTGCCGGATCCCTGAAAGTCCCTGCCCCTGCCTCTCGCCCTAAGCCCCGGCCCAGCCCGAGCAGCACCCGAGAACCCCTCCTCAGCAGCTCTGA GAACGGCAATGGTGGCCGAGAGACGTCTCCAGCTGATGCCCGGACCGAGAGAATCCTCAGTGACCTCCTGCCCTTCTGCAGACCAGGCCCCGAGGGCCAG CTCGCTGCCACGGCCCTTCGGAAAGTGCTGTCCCTGAAGCAGCTTCTGTTTCCGGCGGAGGAAGACAATGGGGCGGGGCCTCCTCGAGATGGGGATGGGG TCCCAGGGGGCGGCCCCCTGAGCCCAGCACGGACCCAGGAAATCCAGGAGAACCTGCTCAGCTTGGAGGAGACCATGAAGCAGCTGGAGGAGTTGGAGGA GGAATTTTGCCGCCTGAGACCCCTCCTGTCTCAGCTTGGGGGGAACTCTGTCCCCCAGCCTGGCTGCACTTGAGGTTCCCGCCCAGGAAGGCCTTTTGCA AGAAGGAGAGGAATGGGGGAGAGGACGTGAGGGACCACCCCCACCCACACAGCTGCCGCAGCATCTCACACCCCGAGGGCCTGAGGAGAGGGAGCTGTGG GCCACGCCTGGGAGGGGCCCAGCTGGGGTTACTGGCCCCGCATGAGCCTCGGCCATCTCTCCCTCCTGCCCTCTGCTTGGGGGACTCAGGGCTCCATTCT GGAGGGCACCACGGTGACCCGGGCCATCTCAGTATTGCCTGTGGGGGCCACCCCTCCACCCCCACCCCCAAGTGCCTTCGCTCTGTTTTTATACCCTGAA TTGGAGGTTTATTTTTTAATATATATTATCTAAGAAGA

refseq_ARHGEF1.F2 (forward primer)
refseq_ARHGEF1.R2 (reverse primer)
Single exon skipping

Frame 0

GGLRAARATRRQGWGECGARARRLRFRWRRWLL FPPGAALQSPGRWKTSPEGRPPQAPPGLAWFPSA SSGLRMRILRTSWRQTQKSKTASSRAWSR*SGA QPTSWPSCSTWPCSLSQDPCFAVCMPTCWAHWA PRRPRRPSWTSTTASWRRQRFSGCRSLPTSPLNL TALGLTSSPRMSSGGSCRRWCKASR*PWAGSWR TSVPSGSWA*RPGSRSWPSWRLGLGGTEPATRP GSGTWRSGCSCTWRRCNIPSLPTKKRVLPWSTPL ACTCATLGCGPRVETRSRGGTSSGKR*WGTGGR TSLPRPRRG*AASWMPPAGTGESPRFQIFDTSK QRLMPRSQVLQTGREAWGCPLGTGISGLLGRTPL ESLCTLCPWTAQTGNQVLTPPWSWGTHPRRAQ* AWSPWRPQRVPTRGPKPRAPSLEMRGSRGGRDW SLNQKSLPAGGNSSPQTPCTACPRAR*SGRRSSA SCW*QRRPTCACCGCCTTSSSSPWQNACSSPWR SCRTSSPAWTSSSRCIPCSSIA**SGGRRVATS SRRSETCCWPGLMVLRAPGSRKSPPASAAASHLP *SSSKPSNARTLGSVPSCRKLRAARGAAACS*R T*SPRRCSG*PSTPCSCRASGRTQKSPQNGRKW SWQPSAAGKFYTTSTKPCVTWRTC*GSRTISGAW TCPTFGRAATLC*ASSRTWTSPRRNWSTRAH*R GG*LRTRQWRCMCCCWTTCCCCSSARTSGCCSS PIAGH*RPRPMARPCCGPCCGSPPP*PARWPPIT KPSTSFLPGTRRPRYTSWWHRLCRSGKTGVLSS LRLPDP*KSLPLPLALSPGPARAAPENPSSAAL RTAMVAERRLQLMPGPRESSVTSCPSADQAPRAS SLPRPFGKCCP*SSFCFRRRKTMGRGLLEMGMG SQGAAP*AQHGPRKSRRTCSAWRRP*SSWRSWR RNFAA*DPSCLSLGGTLSPSLAALEVPAQEGLLQ EGEEWGRGREGPPPPTQLPQHLTPRGPEERELW ATPGRGPAGVTGPA*ASAISPSCPLLGGLRAPF WRAPR*PGPSQYCLWGPPLHPHPQVPSLCFYTLN WRFIF*YILSKK

Frame 1

VDSGRLERRGGRGGESAEPERGGFGSGGGDGFS FHLEQPCRAQGDGRLRPRGGLPRPLPAWPGSRQ HHRG*G*GF*ERAGDKLRRAKQPVPEPGAGEAAP SPPHGPPAARGPAV*ARTPALLSACRHAGLTGP QGGQEGLPGLLPQLPGEDSGSPGAGPSQRRL*T *PH*G*PHLRGCPAAVRAGGGAKPAGSRGPAAGG LPFQAAHGHDALGAGAGPAGGLGWAGPSQLRGP GAARGGAAAHAPGGDATYHLYRRRKECCRGQRH WPVHAPPWGADQEWRQEVGEELLPEKGDGEPAVG RACQDQEGAEQHPGCRPLEPGRAPGSRFSTPQS RG*CREARCYRPEGRRGDALSGPEYRGSWAGHP WSLSAPSVPGQPRPGTRC*RPPGAGGLIPAGPNE PGVLGAPREYRRGGRNREPRAWR*GGAGAVGTG A*TRRASRLAGTRPPRHPAQPAQEPGEAAGGHQ RAAGDRGGPRAHAAGAARPLLPAHGRMPVLPLGG AAEHLPQPGRAHRGAFPVPRSPDEAEAGEWLPH RGDRRRAAGPV*WC*GLLVPENLLPLLQPPVIC LRAAQSQATQGPSVLCLRAGS*EPPAVPPPAAEG HDPHGDAAADQVPPAPAEHRAEHRRAHRTGESG AGSRVLPGNSTPRQPSRA*HGGPAEAQGLSAAP GLVPPSAEQRPYAERVQEPGHHQEEIGPRGPTDV AGD*GQGSGGACAAAGRPAAAAPAPGRAAAAQV P*PDTDAHARWQDHAAARAAAHLRHDPRGGHRS QSLLRPFYLGPGGPDIRAGGTDCVGAEKLVCSHH *DCRIPESPCPCLSP*APAQPEQHPRTPPQQL* ERQWWPRDVSS*CPDRENPQ*PPALLQTRPRGP ARCHGPSESAVPEAASVSGGGRQWGGASSRWGWG PRGRPPEPSTDPGNPGEPAQLGGDHEAAGGVGG GILPPETPPVSAWGELCPPAWLHLRFPPRKAFC KKERNGGEDVRDHPHPHSCRSISHPEGLRRGSCG PRLGGAQLGLLAPHEPRPSLPPALCLGDSGLHS GGHHGDPGHLSIACGGHPSTPTPKCLRSVFIP* IGGLFFNIYYLRR

Frame 2

WTQGG*SDAAAGVGRVRSPSAEASVPVAAMASL STWSSPAEPREMEDFARGAASPGPSRPGLVPVS IIGAEDEDFENELETNSEEQNSQFQSLEQVKRR PAHLMALLQHVALQFEPGPLLCCLHADMLGSLGP KEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFEL DRTRADLISEDVQRRFVQEVVQSQQVAVGRQLE DFRSKRLMGMTPWEQELAQLEAWVGRDRASYEAR ERHVAERLLMHLEEMQHTISTDEEKSAAVVNAI GLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRS DEPAKTKKGLSSILDAARWNRGEPQVPDFRHLKA EVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTP GVSLHPLSLDSPDREPGADAPLELGDSSPQGPM SLESLAPPESTDEGAETESPEPGDEGEPGRSGLE LEPEEPPGWRELVPPDTLHSLPKSQVKRQEVIS ELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLE ELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLI EEIGDVLLARFDGAEGSWFQKISSRFCSRQSFA LEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLK DMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVE LAAECCREILHHVNQAVRDMEDLLRLKDYQRRL DLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLT WRVTKDKAVEVHVLLLDDLLLLLQRQDERLLLKS HSRTLTPTPDGKTMLRPVLRLTSAMTREVATDH KAFYVLFTWDQEAQIYELVAQTVSERKNWCALI TETAGSLKVPAPASRPKPRPSPSSTREPLLSSSE NGNGGRETSPADARTERILSDLLPFCRPGPEGQ LAATALRKVLSLKQLLFPAEEDNGAGPPRDGDG VPGGGPLSPARTQEIQENLLSLEETMKQLEELEE EFCRLRPLLSQLGGNSVPQPGCT*GSRPGRPFA RRRGMGERT*GTTPTHTAAAASHTPRA*GEGAV GHAWEGPSWGYWPRMSLGHLSLLPSAWGTQGSIL EGTTVTRAISVLPVGATPPPPPPSAFALFLYPE LEVYFLIYII*E