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Sequences

Sequence NM_006125

refseq_ARHGAP6.F1 (forward primer)
refseq_ARHGAP6.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_013427

GGCTGGGCTGCGAATAGCGTGTTCCTCTCCGGCGGAACACACACACCCGGCCTTGGGGCTGTCTCCTGAGCTCCCTCCTCCACGGAGAGCGCTGAGCGCC GCCGGGAATTCCATCCCACCGTGGGCACGCAGTCTTTGGAGGTCCCGGGCGCAGCACGCTCGGTGTCCCCACACTGCAGCAAGACAGAGACCCCGCGGGA ACCTTGAGCTTGGAACAACCCTTGAGCCTCTGCAGTCGGAAGAGTGGGCGCAGCAGCCCAGCGGAGGCCAGGCGCGCAACCTCGGGCGCCGGGGCAAGGA GAGAGTGCAGGGAGGCGCAGCTCAGGCGCCCGGCTCAGGAGCGGGAGGAAGTTCTCGCGGCGCCGGGAGCGCGGTGGACGCGCCCTGGGCGCACGCCCAG GCAGCCTTCTCCCTGGCCCTCGGGACTGTCCTCGGGCCGCAAGGAGGAGCTTGCTGGAGTCTTAGAGGCCATCCAGAGCCAGCGAGCAGGAGCGCTGCGT CTCCCGCCTCAGCTAGGAAGGGGGAGTGGCGCTGGCAGGCTGGAGCTGGGAACCCAGCGAGCGCCTGACCTTCCTCCTCCTCTTCCTGACCCTCTTCGCG TCTTGGGCTCCGGAGGAAGGTTCTAGCGGCTGCAGGAGGTCCCCAGACCCATTTTCCTAGAAGGCTGGTGATGGATCTGCTGCTCCTGCCGCCGCCGGGG CACTTGGAGCGCACCGGCGGCGCGTGAGCTGGGCTTTGCTCTCCACTGCCCTGGGCAAACCCCGGGCCAGCCCCGCCTGGCACCTTTGCCTGAGTCCCTT TCGGTTCCCGACCCAAAGCCACCAGCGTCCAGGGAGGGAGGAGGAGGTGGTCCTCAGGTGCAGCCCCGCCGAGATGTCCGCGCAGAGCCTGCTCCACAGC GTCTTCTCCTGTTCCTCGCCCGCTTCAAGTAGCGCGGCCTCGGCCAAGGGCTTCTCCAAGAGGAAGCTGCGCCAGACCCGCAGCCTGGACCCGGCCCTGA TCGGCGGCTGCGGGAGCGACGAGGCGGGCGCGGAGGGCAGTGCGCGGGGAGCCACGGCGGGCCGCCTCTACTCCCCATCACTCCCAGCCGAGAGTCTCGG CCCTCGCTTGGCGTCCTCTTCCCGGGGTCCGCCCCCCAGGGCCACCAGGCTACCGCCTCCTGGACCTCTTTGCTCGTCCTTCTCCACACCCAGCACCCCG CAGGAGAAGTCACCATCCGGCAGCTTTCACTTTGACTATGAGGTTCCCCTGGGTCGCGGCGGCCTCAAGAAGAGCATGGCCTGGGACCTGCCTTCTGTCC TGGCCGGGCCAGCCAGTAGCCGAAGCGCTTCCAGCATCCTCTGTTCATCCGGGGGAGGCCCCAATGGCATCTTCGCTTCTCCTAGGAGGTGGCTCCAGCA GAGGAAGTTCCAGTCCCCACCCGACAGTCGCGGGCACCCCTACGTCGTGTGGAAATCCGAGGGTGATTTCACCTGGAACAGCATGTCAGGCCGCAGTGTA CGGCTGAGGTCAGTCCCCATCCAGAGTCTCTCAGAGCTGGAGAGGGCCCGGCTGCAGGAAGTGGCTTTTTATCAGTTGCAACAGGACTGTGACCTGAGCT GTCAGATCACCATTCCCAAAGATGGACAAAAGAGAAAGAAATCTTTAAGAAAGAAACTGGATTCACTAGGAAAGGAGAAAAACAAAGACAAAGAATTCAT CCCACAGGCATTTGGAATGCCCTTATCCCAAGTCATTGCGAATGACAGGGCCTATAAACTCAAGCAGGACTTGCAGAGGGACGAGCAGAAAGATGCATCT GACTTTGTGGCTTCCCTCCTCCCATTTGGAAATAAAAGACAAAACAAAGAACTCTCAAGCAGTAACTCATCTCTCAGCTCAACCTCAGAAACACCGAATG AGTCAACGTCCCCAAACACCCCGGAACCGGCTCCTCGGGCTAGGAGGAGGGGTGCCATGTCAGTGGATTCTATCACCGATCTTGATGACAATCAGTCTCG ACTACTAGAAGCTTTACAACTTTCCTTGCCTGCTGAGGCTCAAAGTAAAAAGGAAAAAGCCAGAGATAAGAAACTCAGTCTGAATCCTATTTACAGACAG GTCCCTAGGCTGGTGGACAGCTGCTGTCAGCACCTAGAAAAACATGGCCTCCAGACAGTGGGGATATTCCGAGTTGGAAGCTCAAAAAAGAGAGTGAGAC AATTACGTGAGGAATTTGACCGTGGGATTGATGTCTCTCTGGAGGAGGAGCACAGTGTTCATGATGTGGCAGCCTTGCTGAAAGAGTTCCTGAGGGACAT GCCAGACCCCCTTCTCACCAGGGAGCTGTACACAGCTTTCATCAACACTCTCTTGTTGGAGCCGGAGGAACAGCTGGGCACCTTGCAGCTCCTCATATAC CTTCTACCTCCCTGCAACTGCGACACCCTCCACCGCCTGCTACAGTTCCTCTCCATCGTGGCCAGGCATGCCGATGACAACATCAGCAAAGATGGGCAAG AGGTCACTGGGAATAAAATGACATCTCTAAACTTAGCCACCATATTTGGACCCAACCTGCTGCACAAGCAGAAGTCATCAGACAAAGAATTCTCAGTTCA GAGTTCAGCCCGGGCTGAGGAGAGCACGGCCATCATCGCTGTTGTGCAAAAGATGATTGAAAATTATGAAGCCCTGTTCATGGTTCCCCCAGATCTCCAG AACGAAGTGCTGATCAGCCTGTTAGAGACCGATCCTGATGTCGTGGACTATTTACTCAGAAGAAAGGCTTCCCAATCATCAAGCCCTGACATGCTGCAGT CGGAAGTTTCCTTTTCCGTGGGAGGGAGGCATTCATCTACAGACTCCAACAAGGCCTCCAGCGGAGACATCTCCCCTTATGACAACAACTCCCCAGTGCT GTCTGAGCGCTCCCTGCTGGCTATGCAAGAGGACGCGGCCCCGGGGGGCTCGGAGAAGCTTTACAGAGTGCCAGGGCAGTTTATGCTGGTGGGCCACTTG TCGTCGTCAAAGTCAAGGGAAAGTTCTCCTGGACCAAGGCTTGGGAAAGATCTGTCAGAGGAGCCTTTCGATATCTGGGGAACTTGGCATTCAACATTAA AAAGCGGATCCAAAGACCCAGGAATGACAGGTTCCTCTGGAGACATTTTTGAAAGCAGCTCCCTAAGAGCGGGGCCCTGCTCCCTTTCTCAAGGGAACCT GTCCCCAAATTGGCCTCGGTGGCAGGGGAGCCCCGCAGAGCTGGACAGCGACACGCAGGGGGCTCGGAGGACTCAGGCCGCAGCCCCCGCGACGGAGGGC AGGGCCCACCCTGCGGTGTCGCGCGCCTGCAGCACGCCCCACGTCCAGGTGGCAGGGAAAGCCGAGCGGCCCACGGCCAGGTCGGAGCAGTACTTGACCC TGAGCGGCGCCCACGACCTCAGCGAGAGTGAGCTGGATGTGGCCGGGCTGCAGAGCCGGGCCACACCTCAGTGCCAAAGACCCCATGGGAGTGGGAGGGA TGACAAGCGGCCCCCGCCTCCATACCCGGGCCCAGGGAAGCCCGCGGCAGCGGCAGCCTGGATCCAGGGGCCCCCGGAAGGCGTGGAGACACCCACGGAC CAGGGAGGCCAAGCAGCCGAGCGAGAGCAGCAGGTCACGCAGAAAAAACTGAGCAGCGCCAACTCCCTGCCAGCGGGCGAGCAGGACAGTCCGCGCCTGG GGGACGCTGGCTGGCTCGACTGGCAGAGAGAGCGCTGGCAGATCTGGGAGCTCCTGTCGACCGACAACCCCGATGCCCTGCCCGAGACGCTGGTCTGAGC CCGCACCCAGCCGAGCCCCCCCTGCCCCGAGCCCCCCGCCCTCCAGCCCAGGGGGGACCGTGGGTGGTGGCCACTGGCACACTTAGTGTTCTTCTTTCAC ACTTCTCAAAAGTGACACAAGAGAAATCCAGTTCACCTACAGAGGTAGAGCACTCACGCCCCCGCCATTGAGAATAAGGTTCCATTGCGTAGCCAGCCTT AGGAAAAACAAACAGAACCCAAACCAGATGGCAATGTCCAATCTAAAAACGTCCCTCTTGGCTCTATAATATAAGATACAACTCTTGCTTGGTATAGCCT AACCGTATTTATGTGTCTTCGGTTTTGACTATTGTGTATTCTGTAACAGATTATGTATAATCATATATGATATATTCACAAAGAGAAAACAAAAGGAACT TTTAAAAAAAAAATCACTTCACTTATATTAAGCAATGAGATATACTAAACAATGAGATTCTATAGAATGTTCTAGAATGTGCACAAGCGGGTTTCTGTGC TTTTGCCATAGCTTTATAACTGGGGATAACCCTTCCTTCGATACCAAACACTAACAAGAGGAAGCAGAATATGAGAAGCCATATTTTTACATAGGAGTCA GATACAAAAAGAAAAATCACTGAATGCTTTTAGATATTGAATACGTTTTCAGGAAAATGCTAAATCTGATAGATTACGAAATATATTTTTAGAACTTGTT TAGAAAGGATTCAGTTAACCAAACAAGAAAAAGGCAGTGCCTCACAAAGAAATTAAGAAGTTGTCCGTCCCACGTTACATCAAATTCAGTTTTATATAGG CCATATATAATATATATTTATAATGTATAATTTTTATGTATTTTTCAAAACTACAAACTGGAATCCAACTATAAAGTGTTTAAGAATCTACACAGAATAT TCAAATTATAGAACATGTTTTTTCCCTTTGCCCCATAATCAGTATTTGCCAAATTACATGCAATTCCTTAAAAACTAAATCACATTTGGTAAAAGGCCTA CAGCTTTGTACTTACATTGTGCCAAAGGCTGAGGAAATGTTTTCTTTCGTAATTTTATGTGTATTGTAAAATGTTCTACCGTACTTTAGTAGTTTGAAGT TTTTCAAGTGCATAACTATTTTTGACCAGCAGATGGCGATACGCTTCAGTATTTTATGCAATTTTTTTTCACTTCTGAAGGGAAAGTGTATTATAAAAAA AGATTTTTTTTTTTTTTATAAAACATGCTACTCTTAATTTTCATGTTGGTGATGAAATTCCCAGTGGTGTTTCTTAAGGTTCTATCTTGTGCCATGATGA ATAAAAAGTTAAGCAAA

refseq_ARHGAP6.F1 (forward primer)
refseq_ARHGAP6.R1 (reverse primer)
Single exon skipping

Frame 0

GWAANSVFLSGGTHTPGLGAVS*APSSTESAER RREFHPTVGTQSLEVPGAARSVSPHCSKTETPRE P*AWNNP*ASAVGRVGAAAQRRPGAQPRAPGQG ESAGRRSSGARLRSGRKFSRRRERGGRALGARP GSLLPGPRDCPRAARRSLLES*RPSRASEQERCV SRLS*EGGVALAGWSWEPSERLTFLLLFLTLFA SWAPEEGSSGCRRSPDPFS*KAGDGSAAPAAAG ALGAHRRRVSWALLSTALGKPRASPAWHLCLSPF RFPTQSHQRPGREEEVVLRCSPAEMSAQSLLHS VFSCSSPASSSAASAKGFSKRKLRQTRSLDPAL IGGCGSDEAGAEGSARGATAGRLYSPSLPAESLG PRLASSSRGPPPRATRLPPPGPLCSSFSTPSTP QEKSPSGSFHFDYEVPLGRGGLKKSMAWDLPSV LAGPASSRSASSILCSSGGGPNGIFASPRRWLQQ RKFQSPPDSRGHPYVVWKSEGDFTWNSMSGRSV RLRSVPIQSLSELERARLQEVAFYQLQQDCDLS CQITIPKDGQKRKKSLRKKLDSLGKEKNKDKEFI PQAFGMPLSQVIANDRAYKLKQDLQRDEQKDAS DFVASLLPFGNKRQNKELSSSNSSLSSTSETPN ESTSPNTPEPAPRARRRGAMSVDSITDLDDNQSR LLEALQLSLPAEAQSKKEKARDKKLSLNPIYRQ VPRLVDSCCQHLEKHGLQTVGIFRVGSSKKRVR QLREEFDRGIDVSLEEEHSVHDVAALLKEFLRDM PDPLLTRELYTAFINTLLLEPEEQLGTLQLLIY LLPPCNCDTLHRLLQFLSIVARHADDNISKDGQ EVTGNKMTSLNLATIFGPNLLHKQKSSDKEFSVQ SSARAEESTAIIAVVQKMIENYEALFMVPPDLQ NEVLISLLETDPDVVDYLLRRKASQSSSPDMLQ SEVSFSVGGRHSSTDSNKASSGDISPYDNNSPVL SERSLLAMQEDAAPGGSEKLYRVPGQFMLVGHL SSSKSRESSPGPRLGKDLSEEPFDIWGTWHSTL KSGSKDPGMTGSSGDIFESSSLRAGPCSLSQGNL SPNWPRWQGSPAELDSDTQGARRTQAAAPATEG RAHPAVSRACSTPHVQVAGKAERPTARSEQYLT LSGAHDLSESELDVAGLQSRATPQCQRPHGSGRD DKRPPPPYPGPGKPAAAAAWIQGPPEGVETPTD QGGQAAEREQQVTQKKLSSANSLPAGEQDSPRL GDAGWLDWQRERWQIWELLSTDNPDALPETLV*A RTQPSPPCPEPPALQPRGDRGWWPLAHLVFFFH TSQK*HKRNPVHLQR*STHAPAIENKVPLRSQP *EKQTEPKPDGNVQSKNVPLGSII*DTTLAWYSL TVFMCLRF*LLCIL*QIMYNHI*YIHKEKTKGT FKKKITSLILSNEIY*TMRFYRMF*NVHKRVSV LLP*LYNWG*PFLRYQTLTRGSRI*EAIFLHRSQ IQKEKSLNAFRY*IRFQENAKSDRLRNIFLELV *KGFS*PNKKKAVPHKEIKKLSVPRYIKFSFI* AIYNIYL*CIIFMYFSKLQTGIQL*SV*ESTQNI QIIEHVFSLCPIISICQITCNSLKTKSHLVKGL QLCTYIVPKAEEMFSFVILCVL*NVLPYFSSLK FFKCITIFDQQMAIRFSILCNFFSLLKGKCIIKK DFFFFYKTCYS*FSCW**NSQWCFLRFYLVP** IKS*A

Frame 1

AGLRIACSSPAEHTHPALGLSPELPPPRRALSA AGNSIPPWARSLWRSRAQHARCPHTAARQRPRG NLELGTTLEPLQSEEWAQQPSGGQARNLGRRGKE RVQGGAAQAPGSGAGGSSRGAGSAVDAPWAHAQ AAFSLALGTVLGPQGGACWSLRGHPEPASRSAA SPASARKGEWRWQAGAGNPASA*PSSSSS*PSSR LGLRRKVLAAAGGPQTHFPRRLVMDLLLLPPPG HLERTGGA*AGLCSPLPWANPGPAPPGTFA*VP FGSRPKATSVQGGRRRWSSGAAPPRCPRRACSTA SSPVPRPLQVARPRPRASPRGSCARPAAWTRP* SAAAGATRRARRAVRGEPRRAASTPHHSQPRVS ALAWRPLPGVRPPGPPGYRLLDLFARPSPHPAPR RRSHHPAAFTLTMRFPWVAAASRRAWPGTCLLS WPGQPVAEALPASSVHPGEAPMASSLLLGGGSS RGSSSPHPTVAGTPTSCGNPRVISPGTACQAAVY G*GQSPSRVSQSWRGPGCRKWLFISCNRTVT*A VRSPFPKMDKRERNL*ERNWIH*ERRKTKTKNS SHRHLECPYPKSLRMTGPINSSRTCRGTSRKMHL TLWLPSSHLEIKDKTKNSQAVTHLSAQPQKHRM SQRPQTPRNRLLGLGGGVPCQWILSPILMTISL DY*KLYNFPCLLRLKVKRKKPEIRNSV*ILFTDR SLGWWTAAVST*KNMASRQWGYSELEAQKRE*D NYVRNLTVGLMSLWRRSTVFMMWQPC*KSS*GT CQTPFSPGSCTQLSSTLSCWSRRNSWAPCSSSYT FYLPATATPSTACYSSSPSWPGMPMTTSAKMGK RSLGIK*HL*T*PPYLDPTCCTSRSHQTKNSQF RVQPGLRRARPSSLLCKR*LKIMKPCSWFPQISR TKC*SAC*RPILMSWTIYSEERLPNHQALTCCS RKFPFPWEGGIHLQTPTRPPAETSPLMTTTPQC CLSAPCWLCKRTRPRGARRSFTECQGSLCWWATC RRQSQGKVLLDQGLGKICQRSLSISGELGIQH* KADPKTQE*QVPLETFLKAAP*ERGPAPFLKGT CPQIGLGGRGAPQSWTATRRGLGGLRPQPPRRRA GPTLRCRAPAARPTSRWQGKPSGPRPGRSST*P *AAPTTSARVSWMWPGCRAGPHLSAKDPMGVGG MTSGPRLHTRAQGSPRQRQPGSRGPRKAWRHPRT REAKQPSESSRSRRKN*AAPTPCQRASRTVRAW GTLAGSTGRESAGRSGSSCRPTTPMPCPRRWSE PAPSRAPPAPSPPPSSPGGTVGGGHWHT*CSSFT LLKSDTREIQFTYRGRALTPPPLRIRFHCVASL RKNKQNPNQMAMSNLKTSLLAL*YKIQLLLGIA *PYLCVFGFDYCVFCNRLCIIIYDIFTKRKQKEL LKKKSLHLY*AMRYTKQ*DSIECSRMCTSGFLC FCHSFITGDNPSFDTKH*QEEAEYEKPYFYIGV RYKKKNH*MLLDIEYVFRKMLNLIDYEIYF*NLF RKDSVNQTRKRQCLTKKLRSCPSHVTSNSVLYR PYIIYIYNV*FLCIFQNYKLESNYKVFKNLHRI FKL*NMFFPFAP*SVFAKLHAIP*KLNHIW*KAY SFVLTLCQRLRKCFLS*FYVYCKMFYRTLVV*S FSSA*LFLTSRWRYASVFYAIFFHF*RESVL*K KIFFFFIKHATLNFHVGDEIPSGVS*GSILCHDE *KVKQ

Frame 2

LGCE*RVPLRRNTHTRPWGCLLSSLLHGER*AP PGIPSHRGHAVFGGPGRSTLGVPTLQQDRDPAG TLSLEQPLSLCSRKSGRSSPAEARRATSGAGAR RECREAQLRRPAQEREEVLAAPGARWTRPGRTPR QPSPWPSGLSSGRKEELAGVLEAIQSQRAGALR LPPQLGRGSGAGRLELGTQRAPDLPPPLPDPLR VLGSGGRF*RLQEVPRPIFLEGW*WICCSCRRRG TWSAPAARELGFALHCPGQTPGQPRLAPLPESL SVPDPKPPASREGGGGGPQVQPRRDVRAEPAPQ RLLLFLARFK*RGLGQGLLQEEAAPDPQPGPGPD RRLRERRGGRGGQCAGSHGGPPLLPITPSRESR PSLGVLFPGSAPQGHQATASWTSLLVLLHTQHP AGEVTIRQLSL*L*GSPGSRRPQEEHGLGPAFCP GRASQ*PKRFQHPLFIRGRPQWHLRFS*EVAPA EEVPVPTRQSRAPLRRVEIRG*FHLEQHVRPQC TAEVSPHPESLRAGEGPAAGSGFLSVATGL*PEL SDHHSQRWTKEKEIFKKETGFTRKGEKQRQRIH PTGIWNALIPSHCE*QGL*TQAGLAEGRAERCI *LCGFPPPIWK*KTKQRTLKQ*LISQLNLRNTE* VNVPKHPGTGSSG*EEGCHVSGFYHRS**QSVS TTRSFTTFLAC*GSK*KGKSQR*ETQSESYLQT GP*AGGQLLSAPRKTWPPDSGDIPSWKLKKESET IT*GI*PWD*CLSGGGAQCS*CGSLAERVPEGH ARPPSHQGAVHSFHQHSLVGAGGTAGHLAAPHI PSTSLQLRHPPPPATVPLHRGQACR*QHQQRWAR GHWE*NDISKLSHHIWTQPAAQAEVIRQRILSS EFSPG*GEHGHHRCCAKDD*KL*SPVHGSPRSP ERSADQPVRDRS*CRGLFTQKKGFPIIKP*HAAV GSFLFRGREAFIYRLQQGLQRRHLPL*QQLPSA V*ALPAGYARGRGPGGLGEALQSARAVYAGGPL VVVKVKGKFSWTKAWERSVRGAFRYLGNLAFNIK KRIQRPRNDRFLWRHF*KQLPKSGALLPFSREP VPKLASVAGEPRRAGQRHAGGSEDSGRSPRDGG QGPPCGVARLQHAPRPGGRESRAAHGQVGAVLDP ERRPRPQRE*AGCGRAAEPGHTSVPKTPWEWEG *QAAPASIPGPREARGSGSLDPGAPGRRGDTHG PGRPSSRARAAGHAEKTEQRQLPASGRAGQSAPG GRWLARLAERALADLGAPVDRQPRCPARDAGLS PHPAEPPLPRAPRPPAQGGPWVVATGTLSVLLS HFSKVTQEKSSSPTEVEHSRPRH*E*GSIA*PAL GKTNRTQTRWQCPI*KRPSWLYNIRYNSCLV*P NRIYVSSVLTIVYSVTDYV*SYMIYSQRENKRN F*KKNHFTYIKQ*DILNNEIL*NVLECAQAGFCA FAIAL*LGITLPSIPNTNKRKQNMRSHIFT*ES DTKRKITECF*ILNTFSGKC*I**ITKYIFRTC LERIQLTKQEKGSASQRN*EVVRPTLHQIQFYIG HI*YIFIMYNFYVFFKTTNWNPTIKCLRIYTEY SNYRTCFFPLPHNQYLPNYMQFLKN*ITFGKRP TALYLHCAKG*GNVFFRNFMCIVKCSTVL**FEV FQVHNYF*PADGDTLQYFMQFFFTSEGKVYYKK RFFFFL*NMLLLIFMLVMKFPVVFLKVLSCAMM NKKLSK

Sequence NM_001174

GGCTGGGCTGCGAATAGCGTGTTCCTCTCCGGCGGAACACACACACCCGGCCTTGGGGCTGTCTCCTGAGCTCCCTCCTCCACGGAGAGCGCTGAGCGCC GCCGGGAATTCCATCCCACCGTGGGCACGCAGTCTTTGGAGGTCCCGGGCGCAGCACGCTCGGTGTCCCCACACTGCAGCAAGACAGAGACCCCGCGGGA ACCTTGAGCTTGGAACAACCCTTGAGCCTCTGCAGTCGGAAGAGTGGGCGCAGCAGCCCAGCGGAGGCCAGGCGCGCAACCTCGGGCGCCGGGGCAAGGA GAGAGTGCAGGGAGGCGCAGCTCAGGCGCCCGGCTCAGGAGCGGGAGGAAGTTCTCGCGGCGCCGGGAGCGCGGTGGACGCGCCCTGGGCGCACGCCCAG GCAGCCTTCTCCCTGGCCCTCGGGACTGTCCTCGGGCCGCAAGGAGGAGCTTGCTGGAGTCTTAGAGGCCATCCAGAGCCAGCGAGCAGGAGCGCTGCGT CTCCCGCCTCAGCTAGGAAGGGGGAGTGGCGCTGGCAGGCTGGAGCTGGGAACCCAGCGAGCGCCTGACCTTCCTCCTCCTCTTCCTGACCCTCTTCGCG TCTTGGGCTCCGGAGGAAGGTTCTAGCGGCTGCAGGAGGTCCCCAGACCCATTTTCCTAGAAGGCTGGTGATGGATCTGCTGCTCCTGCCGCCGCCGGGG CACTTGGAGCGCACCGGCGGCGCGTGAGCTGGGCTTTGCTCTCCACTGCCCTGGGCAAACCCCGGGCCAGCCCCGCCTGGCACCTTTGCCTGAGTCCCTT TCGGTTCCCGACCCAAAGCCACCAGCGTCCAGGGAGGGAGGAGGAGGTGGTCCTCAGGTGCAGCCCCGCCGAGATGTCCGCGCAGAGCCTGCTCCACAGC GTCTTCTCCTGTTCCTCGCCCGCTTCAAGTAGCGCGGCCTCGGCCAAGGGCTTCTCCAAGAGGAAGCTGCGCCAGACCCGCAGCCTGGACCCGGCCCTGA TCGGCGGCTGCGGGAGCGACGAGGCGGGCGCGGAGGGCAGTGCGCGGGGAGCCACGGCGGGCCGCCTCTACTCCCCATCACTCCCAGCCGAGAGTCTCGG CCCTCGCTTGGCGTCCTCTTCCCGGGGTCCGCCCCCCAGGGCCACCAGGCTACCGCCTCCTGGACCTCTTTGCTCGTCCTTCTCCACACCCAGCACCCCG CAGGAGAAGTCACCATCCGGCAGCTTTCACTTTGACTATGAGGTTCCCCTGGGTCGCGGCGGCCTCAAGAAGAGCATGGCCTGGGACCTGCCTTCTGTCC TGGCCGGGCCAGCCAGTAGCCGAAGCGCTTCCAGCATCCTCTGTTCATCCGGGGGAGGCCCCAATGGCATCTTCGCTTCTCCTAGGAGGTGGCTCCAGCA GAGGAAGTTCCAGTCCCCACCCGACAGTCGCGGGCACCCCTACGTCGTGTGGAAATCCGAGTTGGAGTTGTATGATCTTCAGATCTTAGGCACAAAACCA CCGATGAATTCCGATACACATCGTAACTTTGACCCCACCGCAACCCTTAGAAATCAGGGTGATTTCACCTGGAACAGCATGTCAGGCCGCAGTGTACGGC TGAGGTCAGTCCCCATCCAGAGTCTCTCAGAGCTGGAGAGGGCCCGGCTGCAGGAAGTGGCTTTTTATCAGTTGCAACAGGACTGTGACCTGAGCTGTCA GATCACCATTCCCAAAGATGGACAAAAGAGAAAGAAATCTTTAAGAAAGAAACTGGATTCACTAGGAAAGGAGAAAAACAAAGACAAAGAATTCATCCCA CAGGCATTTGGAATGCCCTTATCCCAAGTCATTGCGAATGACAGGGCCTATAAACTCAAGCAGGACTTGCAGAGGGACGAGCAGAAAGATGCATCTGACT TTGTGGCTTCCCTCCTCCCATTTGGAAATAAAAGACAAAACAAAGAACTCTCAAGCAGTAACTCATCTCTCAGCTCAACCTCAGAAACACCGAATGAGTC AACGTCCCCAAACACCCCGGAACCGGCTCCTCGGGCTAGGAGGAGGGGTGCCATGTCAGTGGATTCTATCACCGATCTTGATGACAATCAGTCTCGACTA CTAGAAGCTTTACAACTTTCCTTGCCTGCTGAGGCTCAAAGTAAAAAGGAAAAAGCCAGAGATAAGAAACTCAGTCTGAATCCTATTTACAGACAGGTCC CTAGGCTGGTGGACAGCTGCTGTCAGCACCTAGAAAAACATGGCCTCCAGACAGTGGGGATATTCCGAGTTGGAAGCTCAAAAAAGAGAGTGAGACAATT ACGTGAGGAATTTGACCGTGGGATTGATGTCTCTCTGGAGGAGGAGCACAGTGTTCATGATGTGGCAGCCTTGCTGAAAGAGTTCCTGAGGGACATGCCA GACCCCCTTCTCACCAGGGAGCTGTACACAGCTTTCATCAACACTCTCTTGTTGGAGCCGGAGGAACAGCTGGGCACCTTGCAGCTCCTCATATACCTTC TACCTCCCTGCAACTGCGACACCCTCCACCGCCTGCTACAGTTCCTCTCCATCGTGGCCAGGCATGCCGATGACAACATCAGCAAAGATGGGCAAGAGGT CACTGGGAATAAAATGACATCTCTAAACTTAGCCACCATATTTGGACCCAACCTGCTGCACAAGCAGAAGTCATCAGACAAAGAATTCTCAGTTCAGAGT TCAGCCCGGGCTGAGGAGAGCACGGCCATCATCGCTGTTGTGCAAAAGATGATTGAAAATTATGAAGCCCTGTTCATGGTTCCCCCAGATCTCCAGAACG AAGTGCTGATCAGCCTGTTAGAGACCGATCCTGATGTCGTGGACTATTTACTCAGAAGAAAGGCTTCCCAATCATCAACAAGTTCTGTGCTTCCGGCTGC AGTGCAGGCCTGCCCACAGTACCCTGCCAGCATGTTTACGCCCTGACCTGGAGGGACCAGCTAAGGAAGCCCTGACATGCTGCAGTCGGAAGTTTCCTTT TCCGTGGGAGGGAGGCATTCATCTACAGACTCCAACAAGGCCTCCAGCGGAGACATCTCCCCTTATGACAACAACTCCCCAGTGCTGTCTGAGCGCTCCC TGCTGGCTATGCAAGAGGACGCGGCCCCGGGGGGCTCGGAGAAGCTTTACAGAGTGCCAGGGCAGTTTATGCTGGTGGGCCACTTGTCGTCGTCAAAGTC AAGGGAAAGTTCTCCTGGACCAAGGCTTGGGAAAGATCTGTCAGAGGAGCCTTTCGATATCTGGGGAACTTGGCATTCAACATTAAAAAGCGGATCCAAA GACCCAGGAATGACAGGTTCCTCTGGAGACATTTTTGAAAGCAGCTCCCTAAGAGCGGGGCCCTGCTCCCTTTCTCAAGGGAACCTGTCCCCAAATTGGC CTCGGTGGCAGGGGAGCCCCGCAGAGCTGGACAGCGACACGCAGGGGGCTCGGAGGACTCAGGCCGCAGCCCCCGCGACGGAGGGCAGGGCCCACCCTGC GGTGTCGCGCGCCTGCAGCACGCCCCACGTCCAGGTGGCAGGGAAAGCCGAGCGGCCCACGGCCAGGTCGGAGCAGTACTTGACCCTGAGCGGCGCCCAC GACCTCAGCGAGAGTGAGCTGGATGTGGCCGGGCTGCAGAGCCGGGCCACACCTCAGTGCCAAAGACCCCATGGGAGTGGGAGGGATGACAAGCGGCCCC CGCCTCCATACCCGGGCCCAGGGAAGCCCGCGGCAGCGGCAGCCTGGATCCAGGGGCCCCCGGAAGGCGTGGAGACACCCACGGACCAGGGAGGCCAAGC AGCCGAGCGAGAGCAGCAGGTCACGCAGAAAAAACTGAGCAGCGCCAACTCCCTGCCAGCGGGCGAGCAGGACAGTCCGCGCCTGGGGGACGCTGGCTGG CTCGACTGGCAGAGAGAGCGCTGGCAGATCTGGGAGCTCCTGTCGACCGACAACCCCGATGCCCTGCCCGAGACGCTGGTCTGAGCCCGCACCCAGCCGA GCCCCCCCTGCCCCGAGCCCCCCGCCCTCCAGCCCAGGGGGGACCGTGGGTGGTGGCCACTGGCACACTTAGTGTTCTTCTTTCACACTTCTCAAAAGTG ACACAAGAGAAATCCAGTTCACCTACAGAGGTAGAGCACTCACGCCCCCGCCATTGAGAATAAGGTTCCATTGCGTAGCCAGCCTTAGGAAAAACAAACA GAACCCAAACCAGATGGCAATGTCCAATCTAAAAACGTCCCTCTTGGCTCTATAATATAAGATACAACTCTTGCTTGGTATAGCCTAACCGTATTTATGT GTCTTCGGTTTTGACTATTGTGTATTCTGTAACAGATTATGTATAATCATATATGATATATTCACAAAGAGAAAACAAAAGGAACTTTTAAAAAAAAAAT CACTTCACTTATATTAAGCAATGAGATATACTAAACAATGAGATTCTATAGAATGTTCTAGAATGTGCACAAGCGGGTTTCTGTGCTTTTGCCATAGCTT TATAACTGGGGATAACCCTTCCTTCGATACCAAACACTAACAAGAGGAAGCAGAATATGAGAAGCCATATTTTTACATAGGAGTCAGATACAAAAAGAAA AATCACTGAATGCTTTTAGATATTGAATACGTTTTCAGGAAAATGCTAAATCTGATAGATTACGAAATATATTTTTAGAACTTGTTTAGAAAGGATTCAG TTAACCAAACAAGAAAAAGGCAGTGCCTCACAAAGAAATTAAGAAGTTGTCCGTCCCACGTTACATCAAATTCAGTTTTATATAGGCCATATATAATATA TATTTATAATGTATAATTTTTATGTATTTTTCAAAACTACAAACTGGAATCCAACTATAAAGTGTTTAAGAATCTACACAGAATATTCAAATTATAGAAC ATGTTTTTTCCCTTTGCCCCATAATCAGTATTTGCCAAATTACATGCAATTCCTTAAAAACTAAATCACATTTGGTAAAAGGCCTACAGCTTTGTACTTA CATTGTGCCAAAGGCTGAGGAAATGTTTTCTTTCGTAATTTTATGTGTATTGTAAAATGTTCTACCGTACTTTAGTAGTTTGAAGTTTTTCAAGTGCATA ACTATTTTTGACCAGCAGATGGCGATACGCTTCAGTATTTTATGCAATTTTTTTTCACTTCTGAAGGGAAAGTGTATTATAAAAAAAGATTTTTTTTTTT TTTATAAAACATGCTACTCTTAATTTTCATGTTGGTGATGAAATTCCCAGTGGTGTTTCTTAAGGTTCTATCTTGTGCCATGATGAATAAAAAGTTAAGC AAA

refseq_ARHGAP6.F1 (forward primer)
refseq_ARHGAP6.R1 (reverse primer)
Single exon skipping

Frame 0

GWAANSVFLSGGTHTPGLGAVS*APSSTESAER RREFHPTVGTQSLEVPGAARSVSPHCSKTETPRE P*AWNNP*ASAVGRVGAAAQRRPGAQPRAPGQG ESAGRRSSGARLRSGRKFSRRRERGGRALGARP GSLLPGPRDCPRAARRSLLES*RPSRASEQERCV SRLS*EGGVALAGWSWEPSERLTFLLLFLTLFA SWAPEEGSSGCRRSPDPFS*KAGDGSAAPAAAG ALGAHRRRVSWALLSTALGKPRASPAWHLCLSPF RFPTQSHQRPGREEEVVLRCSPAEMSAQSLLHS VFSCSSPASSSAASAKGFSKRKLRQTRSLDPAL IGGCGSDEAGAEGSARGATAGRLYSPSLPAESLG PRLASSSRGPPPRATRLPPPGPLCSSFSTPSTP QEKSPSGSFHFDYEVPLGRGGLKKSMAWDLPSV LAGPASSRSASSILCSSGGGPNGIFASPRRWLQQ RKFQSPPDSRGHPYVVWKSELELYDLQILGTKP PMNSDTHRNFDPTATLRNQGDFTWNSMSGRSVR LRSVPIQSLSELERARLQEVAFYQLQQDCDLSCQ ITIPKDGQKRKKSLRKKLDSLGKEKNKDKEFIP QAFGMPLSQVIANDRAYKLKQDLQRDEQKDASD FVASLLPFGNKRQNKELSSSNSSLSSTSETPNES TSPNTPEPAPRARRRGAMSVDSITDLDDNQSRL LEALQLSLPAEAQSKKEKARDKKLSLNPIYRQV PRLVDSCCQHLEKHGLQTVGIFRVGSSKKRVRQL REEFDRGIDVSLEEEHSVHDVAALLKEFLRDMP DPLLTRELYTAFINTLLLEPEEQLGTLQLLIYL LPPCNCDTLHRLLQFLSIVARHADDNISKDGQEV TGNKMTSLNLATIFGPNLLHKQKSSDKEFSVQS SARAEESTAIIAVVQKMIENYEALFMVPPDLQN EVLISLLETDPDVVDYLLRRKASQSSTSSVLPAA VQACPQYPASMFTP*PGGTS*GSPDMLQSEVSF SVGGRHSSTDSNKASSGDISPYDNNSPVLSERS LLAMQEDAAPGGSEKLYRVPGQFMLVGHLSSSKS RESSPGPRLGKDLSEEPFDIWGTWHSTLKSGSK DPGMTGSSGDIFESSSLRAGPCSLSQGNLSPNW PRWQGSPAELDSDTQGARRTQAAAPATEGRAHPA VSRACSTPHVQVAGKAERPTARSEQYLTLSGAH DLSESELDVAGLQSRATPQCQRPHGSGRDDKRP PPPYPGPGKPAAAAAWIQGPPEGVETPTDQGGQA AEREQQVTQKKLSSANSLPAGEQDSPRLGDAGW LDWQRERWQIWELLSTDNPDALPETLV*ARTQP SPPCPEPPALQPRGDRGWWPLAHLVFFFHTSQK* HKRNPVHLQR*STHAPAIENKVPLRSQP*EKQT EPKPDGNVQSKNVPLGSII*DTTLAWYSLTVFM CLRF*LLCIL*QIMYNHI*YIHKEKTKGTFKKKI TSLILSNEIY*TMRFYRMF*NVHKRVSVLLP*L YNWG*PFLRYQTLTRGSRI*EAIFLHRSQIQKE KSLNAFRY*IRFQENAKSDRLRNIFLELV*KGFS *PNKKKAVPHKEIKKLSVPRYIKFSFI*AIYNI YL*CIIFMYFSKLQTGIQL*SV*ESTQNIQIIE HVFSLCPIISICQITCNSLKTKSHLVKGLQLCTY IVPKAEEMFSFVILCVL*NVLPYFSSLKFFKCI TIFDQQMAIRFSILCNFFSLLKGKCIIKKDFFF FYKTCYS*FSCW**NSQWCFLRFYLVP**IKS*A

Frame 1

AGLRIACSSPAEHTHPALGLSPELPPPRRALSA AGNSIPPWARSLWRSRAQHARCPHTAARQRPRG NLELGTTLEPLQSEEWAQQPSGGQARNLGRRGKE RVQGGAAQAPGSGAGGSSRGAGSAVDAPWAHAQ AAFSLALGTVLGPQGGACWSLRGHPEPASRSAA SPASARKGEWRWQAGAGNPASA*PSSSSS*PSSR LGLRRKVLAAAGGPQTHFPRRLVMDLLLLPPPG HLERTGGA*AGLCSPLPWANPGPAPPGTFA*VP FGSRPKATSVQGGRRRWSSGAAPPRCPRRACSTA SSPVPRPLQVARPRPRASPRGSCARPAAWTRP* SAAAGATRRARRAVRGEPRRAASTPHHSQPRVS ALAWRPLPGVRPPGPPGYRLLDLFARPSPHPAPR RRSHHPAAFTLTMRFPWVAAASRRAWPGTCLLS WPGQPVAEALPASSVHPGEAPMASSLLLGGGSS RGSSSPHPTVAGTPTSCGNPSWSCMIFRS*AQNH R*IPIHIVTLTPPQPLEIRVISPGTACQAAVYG *GQSPSRVSQSWRGPGCRKWLFISCNRTVT*AV RSPFPKMDKRERNL*ERNWIH*ERRKTKTKNSSH RHLECPYPKSLRMTGPINSSRTCRGTSRKMHLT LWLPSSHLEIKDKTKNSQAVTHLSAQPQKHRMS QRPQTPRNRLLGLGGGVPCQWILSPILMTISLDY *KLYNFPCLLRLKVKRKKPEIRNSV*ILFTDRS LGWWTAAVST*KNMASRQWGYSELEAQKRE*DN YVRNLTVGLMSLWRRSTVFMMWQPC*KSS*GTCQ TPFSPGSCTQLSSTLSCWSRRNSWAPCSSSYTF YLPATATPSTACYSSSPSWPGMPMTTSAKMGKR SLGIK*HL*T*PPYLDPTCCTSRSHQTKNSQFRV QPGLRRARPSSLLCKR*LKIMKPCSWFPQISRT KC*SAC*RPILMSWTIYSEERLPNHQQVLCFRL QCRPAHSTLPACLRPDLEGPAKEALTCCSRKFPF PWEGGIHLQTPTRPPAETSPLMTTTPQCCLSAP CWLCKRTRPRGARRSFTECQGSLCWWATCRRQS QGKVLLDQGLGKICQRSLSISGELGIQH*KADPK TQE*QVPLETFLKAAP*ERGPAPFLKGTCPQIG LGGRGAPQSWTATRRGLGGLRPQPPRRRAGPTL RCRAPAARPTSRWQGKPSGPRPGRSST*P*AAPT TSARVSWMWPGCRAGPHLSAKDPMGVGGMTSGP RLHTRAQGSPRQRQPGSRGPRKAWRHPRTREAK QPSESSRSRRKN*AAPTPCQRASRTVRAWGTLAG STGRESAGRSGSSCRPTTPMPCPRRWSEPAPSR APPAPSPPPSSPGGTVGGGHWHT*CSSFTLLKS DTREIQFTYRGRALTPPPLRIRFHCVASLRKNKQ NPNQMAMSNLKTSLLAL*YKIQLLLGIA*PYLC VFGFDYCVFCNRLCIIIYDIFTKRKQKELLKKK SLHLY*AMRYTKQ*DSIECSRMCTSGFLCFCHSF ITGDNPSFDTKH*QEEAEYEKPYFYIGVRYKKK NH*MLLDIEYVFRKMLNLIDYEIYF*NLFRKDS VNQTRKRQCLTKKLRSCPSHVTSNSVLYRPYIIY IYNV*FLCIFQNYKLESNYKVFKNLHRIFKL*N MFFPFAP*SVFAKLHAIP*KLNHIW*KAYSFVL TLCQRLRKCFLS*FYVYCKMFYRTLVV*SFSSA* LFLTSRWRYASVFYAIFFHF*RESVL*KKIFFF FIKHATLNFHVGDEIPSGVS*GSILCHDE*KVK Q

Frame 2

LGCE*RVPLRRNTHTRPWGCLLSSLLHGER*AP PGIPSHRGHAVFGGPGRSTLGVPTLQQDRDPAG TLSLEQPLSLCSRKSGRSSPAEARRATSGAGAR RECREAQLRRPAQEREEVLAAPGARWTRPGRTPR QPSPWPSGLSSGRKEELAGVLEAIQSQRAGALR LPPQLGRGSGAGRLELGTQRAPDLPPPLPDPLR VLGSGGRF*RLQEVPRPIFLEGW*WICCSCRRRG TWSAPAARELGFALHCPGQTPGQPRLAPLPESL SVPDPKPPASREGGGGGPQVQPRRDVRAEPAPQ RLLLFLARFK*RGLGQGLLQEEAAPDPQPGPGPD RRLRERRGGRGGQCAGSHGGPPLLPITPSRESR PSLGVLFPGSAPQGHQATASWTSLLVLLHTQHP AGEVTIRQLSL*L*GSPGSRRPQEEHGLGPAFCP GRASQ*PKRFQHPLFIRGRPQWHLRFS*EVAPA EEVPVPTRQSRAPLRRVEIRVGVV*SSDLRHKT TDEFRYTS*L*PHRNP*KSG*FHLEQHVRPQCTA EVSPHPESLRAGEGPAAGSGFLSVATGL*PELS DHHSQRWTKEKEIFKKETGFTRKGEKQRQRIHP TGIWNALIPSHCE*QGL*TQAGLAEGRAERCI*L CGFPPPIWK*KTKQRTLKQ*LISQLNLRNTE*V NVPKHPGTGSSG*EEGCHVSGFYHRS**QSVST TRSFTTFLAC*GSK*KGKSQR*ETQSESYLQTGP *AGGQLLSAPRKTWPPDSGDIPSWKLKKESETI T*GI*PWD*CLSGGGAQCS*CGSLAERVPEGHA RPPSHQGAVHSFHQHSLVGAGGTAGHLAAPHIPS TSLQLRHPPPPATVPLHRGQACR*QHQQRWARG HWE*NDISKLSHHIWTQPAAQAEVIRQRILSSE FSPG*GEHGHHRCCAKDD*KL*SPVHGSPRSPER SADQPVRDRS*CRGLFTQKKGFPIINKFCASGC SAGLPTVPCQHVYALTWRDQLRKP*HAAVGSFL FRGREAFIYRLQQGLQRRHLPL*QQLPSAV*ALP AGYARGRGPGGLGEALQSARAVYAGGPLVVVKV KGKFSWTKAWERSVRGAFRYLGNLAFNIKKRIQ RPRNDRFLWRHF*KQLPKSGALLPFSREPVPKLA SVAGEPRRAGQRHAGGSEDSGRSPRDGGQGPPC GVARLQHAPRPGGRESRAAHGQVGAVLDPERRP RPQRE*AGCGRAAEPGHTSVPKTPWEWEG*QAAP ASIPGPREARGSGSLDPGAPGRRGDTHGPGRPS SRARAAGHAEKTEQRQLPASGRAGQSAPGGRWL ARLAERALADLGAPVDRQPRCPARDAGLSPHPAE PPLPRAPRPPAQGGPWVVATGTLSVLLSHFSKV TQEKSSSPTEVEHSRPRH*E*GSIA*PALGKTN RTQTRWQCPI*KRPSWLYNIRYNSCLV*PNRIYV SSVLTIVYSVTDYV*SYMIYSQRENKRNF*KKN HFTYIKQ*DILNNEIL*NVLECAQAGFCAFAIA L*LGITLPSIPNTNKRKQNMRSHIFT*ESDTKRK ITECF*ILNTFSGKC*I**ITKYIFRTCLERIQ LTKQEKGSASQRN*EVVRPTLHQIQFYIGHI*Y IFIMYNFYVFFKTTNWNPTIKCLRIYTEYSNYRT CFFPLPHNQYLPNYMQFLKN*ITFGKRPTALYL HCAKG*GNVFFRNFMCIVKCSTVL**FEVFQVH NYF*PADGDTLQYFMQFFFTSEGKVYYKKRFFFF L*NMLLLIFMLVMKFPVVFLKVLSCAMMNKKLS K