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Sequences

Sequence NM_139026

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGCGCCAACGAGCAGTGCCGCGTGGCCTTCGGCC CCAAGGCTGTCGCCTGCACCTTCGCCAGGGAGCACCTGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAGCTGCAGCCGCCT CCTCGTTCCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCCATAGCAGCAGTG CATGGGCGCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACAACCCCAGACCTG CCTTTGGGGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCATGTCGCAACAGTG CGCCAGGACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGGGATGCTCTGTGC AGACACATGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCCCCCGGGAGGACG GGACCCTGAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCAGGTGTGTGGTGG GGACAACAGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAACCTGACCAGTGTC TACATTGCCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCCCTAACACCACCT ACCCCTCCCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTGGGGACCCCTCCA GGAAGATGCTGACATCCAGGTTTACAGGCGGTATGGCGAGGAGTATGGCAACCTCACCCGCCCAGACATCACCTTCACCTACTTCCAGCCTAAGCCACGG CAGGCCTGGGTGTGGGCCGCTGTGCGTGGGCCCTGCTCGGTGAGCTGTGGGGCAGGGCTGCGCTGGGTAAACTACAGCTGCCTGGACCAGGCCAGGAAGG AGTTGGTGGAGACTGTCCAGTGCCAAGGGAGCCAGCAGCCACCAGCGTGGCCAGAGGCCTGCGTGCTCGAACCCTGCCCTCCCTACTGGGCGGTGGGAGA CTTCGGCCCATGCAGCGCCTCCTGTGGGGGCGGCCTGCGGGAGCGGCCAGTGCGCTGCGTGGAGGCCCAGGGCAGCCTCCTGAAGACATTGCCCCCAGCC CGGTGCAGAGCAGGGGCCCAGCAGCCAGCTGTGGCGCTGGAAACCTGCAACCCCCAGCCCTGCCCTGCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCA CATCAGCTGGTGGAGCAGGCCTGGCCTTGGAGAACGAGACCTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGA TGAGAAGCTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCATGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCA TGGGGCAGCATCAGGACGGGGGCTCAAGCTGCACACGTGTGGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTT TCCTGTGCATGGACTCTGCCCTCAGGGTGCCTGTCCAGGAAGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCC GTGCCCTGCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGCGTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAG GACGATGGTGAGGAGATCCTGTTGGACACCCAGTGCCAGGGGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTGCCCACCTAGGTGGA AAGTCATGTCCCTTGGCCCATGTTCGGCCAGCTGTGGCCTTGGCACTGCTAGACGCTCGGTGGCCTGTGTGCAGCTCGACCAAGGCCAGGACGTGGAGGT GGACGAGGCGGCCTGTGCGGCGCTGGTGCGGCCCGAGGCCAGTGTCCCCTGTCTCATTGCCGACTGCACCTACCGCTGGCATGTTGGCACCTGGATGGAG TGCTCTGTTTCCTGTGGGGATGGCATCCAGCGCCGGCGTGACACCTGCCTCGGACCCCAGGCCCAGGCGCCTGTGCCAGCTGATTTCTGCCAGCACTTGC CCAAGCCGGTGACTGTGCGTGGCTGCTGGGCTGGGCCCTGTGTGGGACAGGGTGCCTGTGGCAGGCAGCACCTTGAGCCAACAGGAACCATTGACATGCG AGGCCCAGGGCAGGCAGACTGTGCAGTGGCCATTGGGCGGCCCCTCGGGGAGGTGGTGACCCTCCGCGTCCTTGAGAGTTCTCTCAACTGCAGTGCGGGG GACATGTTGCTGCTTTGGGGCCGGCTCACCTGGAGGAAGATGTGCAGGAAGCTGTTGGACATGACTTTCAGCTCCAAGACCAACACGCTGGTGGTGAGGC AGCGCTGCGGGCGGCCAGGAGGTGGGGTGCTGCTGCGGTATGGGAGCCAGCTTGCTCCTGAAACCTTCTACAGAGAATGTGACATGCAGCTCTTTGGGCC CTGGGGTGAAATCGTGAGCCCCTCGCTGAGTCCAGCCACGAGTAATGCAGGGGGCTGCCGGCTCTTCATTAATGTGGCTCCGCACGCACGGATTGCCATC CATGCCCTGGCCACCAACATGGGCGCTGGGACCGAGGGAGCCAATGCCAGCTACATCTTGATCCGGGACACCCACAGCTTGAGGACCACAGCGTTCCATG GGCAGCAGGTGCTCTACTGGGAGTCAGAGAGCAGCCAGGCTGAGATGGAGTTCAGCGAGGGCTTCCTGAAGGCTCAGGCCAGCCTGCGGGGCCAGTACTG GACCCTCCAATCATGGGTACCGGAGATGCAGGACCCTCAGTCCTGGAAGGGAAAGGAAGGAACCTGAGGGTCATTGAACATTTGTTCCGTGTCTGGCCAG CCCTGGAGGGTTGACCCCTGGTCTCAGTGCTTTCCAATTCGAACTTTTTCCAATCTTAGGTATCTACTTTAGAGTCTTCTCCAATGTCCAAAAGGCTAGG GGGTTGGAGGTGGGGACTCTGGAAAAGCAGCCCCCATTTCCTCGGGTACCAATAAATAAAACATGCAGGCTG

refseq_ADAMTS13.F5 (forward primer)
refseq_ADAMTS13.R5 (reverse primer)
Alternative donor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG LGCRLP*TATILEQGPACSRRSCRPPTLATAPA LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD GAAPRAGLAWSPCSRRQLLSLLSANEQCRVAFG PKAVACTFAREHLDMCQALSCHTDPLDQSSCSRL LVPLLDGTECGVEKWCSKGRCRSLVELTPIAAV HGRWSSWGPRSPCSRSCGGGVVTRRRQCNNPRP AFGGRACVGADLQAEMCNTQACEKTQLEFMSQQC ARTDGQPLRSSPGGASFYHWGAAVPHSQGDALC RHMCRAIGESFIMKRGDSFLDGTRCMPSGPRED GTLSLCVSGSCRTFGCDGRMDSQQVWDRCQVCGG DNSTCSPRKGSFTAGRAREYVTFLTVTPNLTSV YIANHRPLFTHLAVRIGGRYVVAGKMSISPNTT YPSLLEDGRVEYRVALTEDRLPRLEEIRIWGPLQ EDADIQVYRRYGEEYGNLTRPDITFTYFQPKPR QAWVWAAVRGPCSVSCGAGLRWVNYSCLDQARK ELVETVQCQGSQQPPAWPEACVLEPCPPYWAVGD FGPCSASCGGGLRERPVRCVEAQGSLLKTLPPA RCRAGAQQPAVALETCNPQPCPARWEVSEPSSC TSAGGAGLALENETCVPGADGLEAPVTEGPGSVD EKLPAPEPCVGMSCPPGWGHLDATSAGEKAPSP WGSIRTGAQAAHVWTPAAGSCSVSCGRGLMELR FLCMDSALRVPVQEELCGLASKPGSRREVCQAVP CPARWQYKLAACSVSCGRGVVRRILYCARAHGE DDGEEILLDTQCQGLPRPEPQEACSLEPCPPRW KVMSLGPCSASCGLGTARRSVACVQLDQGQDVEV DEAACAALVRPEASVPCLIADCTYRWHVGTWME CSVSCGDGIQRRRDTCLGPQAQAPVPADFCQHL PKPVTVRGCWAGPCVGQGACGRQHLEPTGTIDMR GPGQADCAVAIGRPLGEVVTLRVLESSLNCSAG DMLLLWGRLTWRKMCRKLLDMTFSSKTNTLVVR QRCGRPGGGVLLRYGSQLAPETFYRECDMQLFGP WGEIVSPSLSPATSNAGGCRLFINVAPHARIAI HALATNMGAGTEGANASYILIRDTHSLRTTAFH GQQVLYWESESSQAEMEFSEGFLKAQASLRGQYW TLQSWVPEMQDPQSWKGKEGT*GSLNICSVSGQ PWRVDPWSQCFPIRTFSNLRYLL*SLLQCPKG* GVGGGDSGKAAPISSGTNK*NMQA

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC* ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT APRPAPASPGPPAAAGSC*ACSAPTSSAAWPSA PRLSPAPSPGSTWICARPSPATQTRWTKAAAAA SSFLSWMGQNVAWRSGAPRVAAAPWWS*PP*QQC MGAGLAGVPEVLAPAPAEEVWSPGGGSATTPDL PLGGVHVLVLTSRPRCATLRPARRPSWSSCRNS APGPTASRCAPPLAAPPSTTGVLLYHTAKGMLCA DTCAGPLARASS*SVETASSMGPGVCQVAPGRT GP*ACVCRAAAGHLAVMVGWTPSRYGTGARCVV GTTARAAHGRALSQLAERENMSHF*QLPPT*PVS TLPTTGLSSHTWR*GSEGAMSWLGR*ASPLTPP TPPSWRMVVSSTEWPSPRTGCPAWRRSASGDPS RKMLTSRFTGGMARSMATSPAQTSPSPTSSLSHG RPGCGPLCVGPAR*AVGQGCAG*TTAAWTRPGR SWWRLSSAKGASSHQRGQRPACSNPALPTGRWE TSAHAAPPVGAACGSGQCAAWRPRAAS*RHCPQP GAEQGPSSQLWRWKPATPSPALPGGRCQSPAHA HQLVEQAWPWRTRPVCQGQMAWRLQ*LRGLAP* MRSCLPLSPVSGCHVLQAGAIWMPPLQGRRLPPH GAASGRGLKLHTCGPLRQGRAPSPAGEV*WSCV SCAWTLPSGCLSRKSCVAWQASLGAGGRSARLS RALLGGSTSWRPAA*AVGEGSCGGSCIVPGPMGR TMVRRSCWTPSARGCLARNPRRPAAWSPAHLGG KSCPLAHVRPAVALALLDARWPVCSSTKARTWR WTRRPVRRWCGPRPVSPVSLPTAPTAGMLAPGWS ALFPVGMASSAGVTPASDPRPRRLCQLISASTC PSR*LCVAAGLGPVWDRVPVAGSTLSQQEPLTC EAQGRQTVQWPLGGPSGRW*PSASLRVLSTAVRG TCCCFGAGSPGGRCAGSCWT*LSAPRPTRWW*G SAAGGQEVGCCCGMGASLLLKPSTENVTCSSLG PGVKS*APR*VQPRVMQGAAGSSLMWLRTHGLPS MPWPPTWALGPREPMPATS*SGTPTA*GPQRSM GSRCSTGSQRAARLRWSSARAS*RLRPACGAST GPSNHGYRRCRTLSPGRERKEPEGH*TFVPCLAS PGGLTPGLSAFQFELFPILGIYFRVFSNVQKAR GLEVGTLEKQPPFPRVPINKTCRL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA *W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG RRRAPRRPRLVPLQPPAAAEPAQRQRAVPRGLRP QGCRLHLRQGAPGYVPGPLLPHRPAGPKQLQPP PRSSPGWDRMWRGEVVLQGSLPLPGGADPHSSS AWALV*LGSPKSLLPLLRRRCGHQEAAVQQPQTC LWGACMCWC*PPGRDVQHSGLREDPAGVHVATV RQDRRPAAALLPWRRLLLPLGCCCTTQPRGCSV QTHVPGHWRELHHEAWRQLPRWDPVYAKWPPGGR DPEPVCVGQLQDIWL*W*DGLPAGMGQVPGVWW GQQHVQPTEGLFHSWQSERICHISDSYPQPDQC LHCQPQASLHTLGGEDRRALCRGWEDEHLP*HHL PLPPGGWSCRVQSGPHRGPAAPPGGDPHLGTPP GRC*HPGLQAVWRGVWQPHPPRHHLHLLPA*AT AGLGVGRCAWALLGELWGRAALGKLQLPGPGQEG VGGDCPVPREPAATSVARGLRARTLPSLLGGGR LRPMQRLLWGRPAGAASALRGGPGQPPEDIAPS PVQSRGPAASCGAGNLQPPALPCQVGGVRAQLMH ISWWSRPGLGERDLCARGRWPGGSSD*GAWLRR *EAACP*ALCRDVMSSRLGPSGCHLCRGEGSLP MGQHQDGGSSCTRVDPCGRVVLRLLRARSDGAAF PVHGLCPQGACPGRAVWPGKQAWEPAGGLPGCP VPCSVAVQAGGLQRELWERGRAEDPVLCPGPWG GRW*GDPVGHPVPGAASPGTPGGLQPGALPT*VE SHVPWPMFGQLWPWHC*TLGGLCAARPRPGRGG GRGGLCGAGAARGQCPLSHCRLHLPLACWHLDG VLCFLWGWHPAPA*HLPRTPGPGACAS*FLPALA QAGDCAWLLGWALCGTGCLWQAAP*ANRNH*HA RPRAGRLCSGHWAAPRGGGDPPRP*EFSQLQCG GHVAALGPAHLEEDVQEAVGHDFQLQDQHAGGEA ALRAARRWGAAAVWEPACS*NLLQRM*HAALWA LG*NREPLAESSHE*CRGLPALH*CGSARTDCH PCPGHQHGRWDRGSQCQLHLDPGHPQLEDHSVPW AAGALLGVREQPG*DGVQRGLPEGSGQPAGPVL DPPIMGTGDAGPSVLEGKGRNLRVIEHLFRVWP ALEG*PLVSVLSNSNFFQS*VSTLESSPMSKRLG GWRWGLWKSSPHFLGYQ*IKHAG

Sequence NM_139027

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGCGCAGGACGGGCGCGCTGCGTGTGGGACCCGC CGCGGCCTCAACCCGGGTCCGCGGGGCACCCGCCGGATGCGCAGCCTGGCCTCTACTACAGCGCCAACGAGCAGTGCCGCGTGGCCTTCGGCCCCAAGGC TGTCGCCTGCACCTTCGCCAGGGAGCACCTGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAGCTGCAGCCGCCTCCTCGTT CCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCCATAGCAGCAGTGCATGGGC GCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACAACCCCAGACCTGCCTTTGG GGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCATGTCGCAACAGTGCGCCAGG ACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGGGATGCTCTGTGCAGACACA TGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCCCCCGGGAGGACGGGACCCT GAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCAGGTGTGTGGTGGGGACAAC AGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAACCTGACCAGTGTCTACATTG CCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCCCTAACACCACCTACCCCTC CCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTGGGGACCCCTCCAGGAAGAT GCTGACATCCAGGTTTACAGGCGGTATGGCGAGGAGTATGGCAACCTCACCCGCCCAGACATCACCTTCACCTACTTCCAGCCTAAGCCACGGCAGGCCT GGGTGTGGGCCGCTGTGCGTGGGCCCTGCTCGGTGAGCTGTGGGGCAGGGCTGCGCTGGGTAAACTACAGCTGCCTGGACCAGGCCAGGAAGGAGTTGGT GGAGACTGTCCAGTGCCAAGGGAGCCAGCAGCCACCAGCGTGGCCAGAGGCCTGCGTGCTCGAACCCTGCCCTCCCTACTGGGCGGTGGGAGACTTCGGC CCATGCAGCGCCTCCTGTGGGGGCGGCCTGCGGGAGCGGCCAGTGCGCTGCGTGGAGGCCCAGGGCAGCCTCCTGAAGACATTGCCCCCAGCCCGGTGCA GAGCAGGGGCCCAGCAGCCAGCTGTGGCGCTGGAAACCTGCAACCCCCAGCCCTGCCCTGCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCACATCAGC TGGTGGAGCAGGCCTGGCCTTGGAGAACGAGACCTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGATGAGAAG CTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCATGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCATGGGGCA GCATCAGGACGGGGGCTCAAGCTGCACACGTGTGGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTTTCCTGTG CATGGACTCTGCCCTCAGGGTGCCTGTCCAGGAAGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCCGTGCCCT GCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGCGTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAGGACGATG GTGAGGAGATCCTGTTGGACACCCAGTGCCAGGGGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTGCCCACCTAGGTGGAAAGTCAT GTCCCTTGGCCCATGTTCGGCCAGCTGTGGCCTTGGCACTGCTAGACGCTCGGTGGCCTGTGTGCAGCTCGACCAAGGCCAGGACGTGGAGGTGGACGAG GCGGCCTGTGCGGCGCTGGTGCGGCCCGAGGCCAGTGTCCCCTGTCTCATTGCCGACTGCACCTACCGCTGGCATGTTGGCACCTGGATGGAGTGCTCTG TTTCCTGTGGGGATGGCATCCAGCGCCGGCGTGACACCTGCCTCGGACCCCAGGCCCAGGCGCCTGTGCCAGCTGATTTCTGCCAGCACTTGCCCAAGCC GGTGACTGTGCGTGGCTGCTGGGCTGGGCCCTGTGTGGGACAGGGTGCCTGTGGCAGGCAGCACCTTGAGCCAACAGGAACCATTGACATGCGAGGCCCA GGGCAGGCAGACTGTGCAGTGGCCATTGGGCGGCCCCTCGGGGAGGTGGTGACCCTCCGCGTCCTTGAGAGTTCTCTCAACTGCAGTGCGGGGGACATGT TGCTGCTTTGGGGCCGGCTCACCTGGAGGAAGATGTGCAGGAAGCTGTTGGACATGACTTTCAGCTCCAAGACCAACACGCTGGTGGTGAGGCAGCGCTG CGGGCGGCCAGGAGGTGGGGTGCTGCTGCGGTATGGGAGCCAGCTTGCTCCTGAAACCTTCTACAGAGAATGTGACATGCAGCTCTTTGGGCCCTGGGGT GAAATCGTGAGCCCCTCGCTGAGTCCAGCCACGAGTAATGCAGGGGGCTGCCGGCTCTTCATTAATGTGGCTCCGCACGCACGGATTGCCATCCATGCCC TGGCCACCAACATGGGCGCTGGGACCGAGGGAGCCAATGCCAGCTACATCTTGATCCGGGACACCCACAGCTTGAGGACCACAGCGTTCCATGGGCAGCA GGTGCTCTACTGGGAGTCAGAGAGCAGCCAGGCTGAGATGGAGTTCAGCGAGGGCTTCCTGAAGGCTCAGGCCAGCCTGCGGGGCCAGTACTGGACCCTC CAATCATGGGTACCGGAGATGCAGGACCCTCAGTCCTGGAAGGGAAAGGAAGGAACCTGAGGGTCATTGAACATTTGTTCCGTGTCTGGCCAGCCCTGGA GGGTTGACCCCTGGTCTCAGTGCTTTCCAATTCGAACTTTTTCCAATCTTAGGTATCTACTTTAGAGTCTTCTCCAATGTCCAAAAGGCTAGGGGGTTGG AGGTGGGGACTCTGGAAAAGCAGCCCCCATTTCCTCGGGTACCAATAAATAAAACATGCAGGCTG

refseq_ADAMTS13.F5 (forward primer)
refseq_ADAMTS13.R5 (reverse primer)
Alternative donor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG LGCRLP*TATILEQGPACSRRSCRPPTLATAPA LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD GAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDP PRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKA VACTFAREHLDMCQALSCHTDPLDQSSCSRLLV PLLDGTECGVEKWCSKGRCRSLVELTPIAAVHG RWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFG GRACVGADLQAEMCNTQACEKTQLEFMSQQCAR TDGQPLRSSPGGASFYHWGAAVPHSQGDALCRH MCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTL SLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDN STCSPRKGSFTAGRAREYVTFLTVTPNLTSVYI ANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPS LLEDGRVEYRVALTEDRLPRLEEIRIWGPLQED ADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQA WVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELV ETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFG PCSASCGGGLRERPVRCVEAQGSLLKTLPPARC RAGAQQPAVALETCNPQPCPARWEVSEPSSCTSA GGAGLALENETCVPGADGLEAPVTEGPGSVDEK LPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWG SIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLC MDSALRVPVQEELCGLASKPGSRREVCQAVPCP ARWQYKLAACSVSCGRGVVRRILYCARAHGEDD GEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVM SLGPCSASCGLGTARRSVACVQLDQGQDVEVDE AACAALVRPEASVPCLIADCTYRWHVGTWMECS VSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKP VTVRGCWAGPCVGQGACGRQHLEPTGTIDMRGP GQADCAVAIGRPLGEVVTLRVLESSLNCSAGDM LLLWGRLTWRKMCRKLLDMTFSSKTNTLVVRQRC GRPGGGVLLRYGSQLAPETFYRECDMQLFGPWG EIVSPSLSPATSNAGGCRLFINVAPHARIAIHA LATNMGAGTEGANASYILIRDTHSLRTTAFHGQQ VLYWESESSQAEMEFSEGFLKAQASLRGQYWTL QSWVPEMQDPQSWKGKEGT*GSLNICSVSGQPW RVDPWSQCFPIRTFSNLRYLL*SLLQCPKG*GVG GGDSGKAAPISSGTNK*NMQA

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC* ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT APRPAPASPGPPAAAGSC*ACSAQDGRAACGTR RGLNPGPRGTRRMRSLASTTAPTSSAAWPSAPR LSPAPSPGSTWICARPSPATQTRWTKAAAAASSF LSWMGQNVAWRSGAPRVAAAPWWS*PP*QQCMG AGLAGVPEVLAPAPAEEVWSPGGGSATTPDLPL GGVHVLVLTSRPRCATLRPARRPSWSSCRNSAPG PTASRCAPPLAAPPSTTGVLLYHTAKGMLCADT CAGPLARASS*SVETASSMGPGVCQVAPGRTGP *ACVCRAAAGHLAVMVGWTPSRYGTGARCVVGTT ARAAHGRALSQLAERENMSHF*QLPPT*PVSTL PTTGLSSHTWR*GSEGAMSWLGR*ASPLTPPTP PSWRMVVSSTEWPSPRTGCPAWRRSASGDPSRKM LTSRFTGGMARSMATSPAQTSPSPTSSLSHGRP GCGPLCVGPAR*AVGQGCAG*TTAAWTRPGRSW WRLSSAKGASSHQRGQRPACSNPALPTGRWETSA HAAPPVGAACGSGQCAAWRPRAAS*RHCPQPGA EQGPSSQLWRWKPATPSPALPGGRCQSPAHAHQ LVEQAWPWRTRPVCQGQMAWRLQ*LRGLAP*MRS CLPLSPVSGCHVLQAGAIWMPPLQGRRLPPHGA ASGRGLKLHTCGPLRQGRAPSPAGEV*WSCVSC AWTLPSGCLSRKSCVAWQASLGAGGRSARLSRAL LGGSTSWRPAA*AVGEGSCGGSCIVPGPMGRTM VRRSCWTPSARGCLARNPRRPAAWSPAHLGGKS CPLAHVRPAVALALLDARWPVCSSTKARTWRWTR RPVRRWCGPRPVSPVSLPTAPTAGMLAPGWSAL FPVGMASSAGVTPASDPRPRRLCQLISASTCPS R*LCVAAGLGPVWDRVPVAGSTLSQQEPLTCEAQ GRQTVQWPLGGPSGRW*PSASLRVLSTAVRGTC CCFGAGSPGGRCAGSCWT*LSAPRPTRWW*GSA AGGQEVGCCCGMGASLLLKPSTENVTCSSLGPGV KS*APR*VQPRVMQGAAGSSLMWLRTHGLPSMP WPPTWALGPREPMPATS*SGTPTA*GPQRSMGS RCSTGSQRAARLRWSSARAS*RLRPACGASTGPS NHGYRRCRTLSPGRERKEPEGH*TFVPCLASPG GLTPGLSAFQFELFPILGIYFRVFSNVQKARGL EVGTLEKQPPFPRVPINKTCRL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA *W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG RRRAPRRPRLVPLQPPAAAEPAQRRTGALRVGPA AASTRVRGAPAGCAAWPLLQRQRAVPRGLRPQG CRLHLRQGAPGYVPGPLLPHRPAGPKQLQPPPR SSPGWDRMWRGEVVLQGSLPLPGGADPHSSSAWA LV*LGSPKSLLPLLRRRCGHQEAAVQQPQTCLW GACMCWC*PPGRDVQHSGLREDPAGVHVATVRQ DRRPAAALLPWRRLLLPLGCCCTTQPRGCSVQTH VPGHWRELHHEAWRQLPRWDPVYAKWPPGGRDP EPVCVGQLQDIWL*W*DGLPAGMGQVPGVWWGQ QHVQPTEGLFHSWQSERICHISDSYPQPDQCLHC QPQASLHTLGGEDRRALCRGWEDEHLP*HHLPL PPGGWSCRVQSGPHRGPAAPPGGDPHLGTPPGR C*HPGLQAVWRGVWQPHPPRHHLHLLPA*ATAGL GVGRCAWALLGELWGRAALGKLQLPGPGQEGVG GDCPVPREPAATSVARGLRARTLPSLLGGGRLR PMQRLLWGRPAGAASALRGGPGQPPEDIAPSPVQ SRGPAASCGAGNLQPPALPCQVGGVRAQLMHIS WWSRPGLGERDLCARGRWPGGSSD*GAWLRR*E AACP*ALCRDVMSSRLGPSGCHLCRGEGSLPMGQ HQDGGSSCTRVDPCGRVVLRLLRARSDGAAFPV HGLCPQGACPGRAVWPGKQAWEPAGGLPGCPVP CSVAVQAGGLQRELWERGRAEDPVLCPGPWGGRW *GDPVGHPVPGAASPGTPGGLQPGALPT*VESH VPWPMFGQLWPWHC*TLGGLCAARPRPGRGGGR GGLCGAGAARGQCPLSHCRLHLPLACWHLDGVLC FLWGWHPAPA*HLPRTPGPGACAS*FLPALAQA GDCAWLLGWALCGTGCLWQAAP*ANRNH*HARP RAGRLCSGHWAAPRGGGDPPRP*EFSQLQCGGHV AALGPAHLEEDVQEAVGHDFQLQDQHAGGEAAL RAARRWGAAAVWEPACS*NLLQRM*HAALWALG *NREPLAESSHE*CRGLPALH*CGSARTDCHPCP GHQHGRWDRGSQCQLHLDPGHPQLEDHSVPWAA GALLGVREQPG*DGVQRGLPEGSGQPAGPVLDP PIMGTGDAGPSVLEGKGRNLRVIEHLFRVWPALE G*PLVSVLSNSNFFQS*VSTLESSPMSKRLGGW RWGLWKSSPHFLGYQ*IKHAG

Sequence NM_139025

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGCGCAGGACGGGCGCGCTGCGTGTGGGACCCGC CGCGGCCTCAACCCGGGTCCGCGGGGCACCCGCCGGATGCGCAGCCTGGCCTCTACTACAGCGCCAACGAGCAGTGCCGCGTGGCCTTCGGCCCCAAGGC TGTCGCCTGCACCTTCGCCAGGGAGCACCTGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAGCTGCAGCCGCCTCCTCGTT CCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCCATAGCAGCAGTGCATGGGC GCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACAACCCCAGACCTGCCTTTGG GGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCATGTCGCAACAGTGCGCCAGG ACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGGGATGCTCTGTGCAGACACA TGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCCCCCGGGAGGACGGGACCCT GAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCAGGTGTGTGGTGGGGACAAC AGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAACCTGACCAGTGTCTACATTG CCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCCCTAACACCACCTACCCCTC CCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTGGGGACCCCTCCAGGAAGAT GCTGACATCCAGGTTTACAGGCGGTATGGCGAGGAGTATGGCAACCTCACCCGCCCAGACATCACCTTCACCTACTTCCAGCCTAAGCCACGGCAGGCCT GGGTGTGGGCCGCTGTGCGTGGGCCCTGCTCGGTGAGCTGTGGGGCAGGGCTGCGCTGGGTAAACTACAGCTGCCTGGACCAGGCCAGGAAGGAGTTGGT GGAGACTGTCCAGTGCCAAGGGAGCCAGCAGCCACCAGCGTGGCCAGAGGCCTGCGTGCTCGAACCCTGCCCTCCCTACTGGGCGGTGGGAGACTTCGGC CCATGCAGCGCCTCCTGTGGGGGCGGCCTGCGGGAGCGGCCAGTGCGCTGCGTGGAGGCCCAGGGCAGCCTCCTGAAGACATTGCCCCCAGCCCGGTGCA GAGCAGGGGCCCAGCAGCCAGCTGTGGCGCTGGAAACCTGCAACCCCCAGCCCTGCCCTGCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCACATCAGC TGGTGGAGCAGGCCTGGCCTTGGAGAACGAGACCTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGATGAGAAG CTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCATGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCATGGGGCA GCATCAGGACGGGGGCTCAAGCTGCACACGTGTGGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTTTCCTGTG CATGGACTCTGCCCTCAGGGTGCCTGTCCAGGAAGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCCGTGCCCT GCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGCGTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAGGACGATG GTGAGGAGATCCTGTTGGACACCCAGTGCCAGGGGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTGCCCACCTAGGTGGAAAGTCAT GTCCCTTGGCCCATGTTCGGCCAGCTGTGGCCTTGGCACTGCTAGACGCTCGGTGGCCTGTGTGCAGCTCGACCAAGGCCAGGACGTGGAGGTGGACGAG GCGGCCTGTGCGGCGCTGGTGCGGCCCGAGGCCAGTGTCCCCTGTCTCATTGCCGACTGCACCTACCGCTGGCATGTTGGCACCTGGATGGAGTGCTCTG TTTCCTGTGGGGATGGCATCCAGCGCCGGCGTGACACCTGCCTCGGACCCCAGGCCCAGGCGCCTGTGCCAGCTGATTTCTGCCAGCACTTGCCCAAGCC GGTGACTGTGCGTGGCTGCTGGGCTGGGCCCTGTGTGGGACAGGGTACGCCCAGCCTGGTGCCCCACGAAGAAGCCGCTGCTCCAGGACGGACCACAGCC ACCCCTGCTGGTGCCTCCCTGGAGTGGTCCCAGGCCCGGGGCCTGCTCTTCTCCCCGGCTCCCCAGCCTCGGCGGCTCCTGCCCGGGCCCCAGGAAAACT CAGTGCAGTCCAGTGCCTGTGGCAGGCAGCACCTTGAGCCAACAGGAACCATTGACATGCGAGGCCCAGGGCAGGCAGACTGTGCAGTGGCCATTGGGCG GCCCCTCGGGGAGGTGGTGACCCTCCGCGTCCTTGAGAGTTCTCTCAACTGCAGTGCGGGGGACATGTTGCTGCTTTGGGGCCGGCTCACCTGGAGGAAG ATGTGCAGGAAGCTGTTGGACATGACTTTCAGCTCCAAGACCAACACGCTGGTGGTGAGGCAGCGCTGCGGGCGGCCAGGAGGTGGGGTGCTGCTGCGGT ATGGGAGCCAGCTTGCTCCTGAAACCTTCTACAGAGAATGTGACATGCAGCTCTTTGGGCCCTGGGGTGAAATCGTGAGCCCCTCGCTGAGTCCAGCCAC GAGTAATGCAGGGGGCTGCCGGCTCTTCATTAATGTGGCTCCGCACGCACGGATTGCCATCCATGCCCTGGCCACCAACATGGGCGCTGGGACCGAGGGA GCCAATGCCAGCTACATCTTGATCCGGGACACCCACAGCTTGAGGACCACAGCGTTCCATGGGCAGCAGGTGCTCTACTGGGAGTCAGAGAGCAGCCAGG CTGAGATGGAGTTCAGCGAGGGCTTCCTGAAGGCTCAGGCCAGCCTGCGGGGCCAGTACTGGACCCTCCAATCATGGGTACCGGAGATGCAGGACCCTCA GTCCTGGAAGGGAAAGGAAGGAACCTGAGGGTCATTGAACATTTGTTCCGTGTCTGGCCAGCCCTGGAGGGTTGACCCCTGGTCTCAGTGCTTTCCAATT CGAACTTTTTCCAATCTTAGGTATCTACTTTAGAGTCTTCTCCAATGTCCAAAAGGCTAGGGGGTTGGAGGTGGGGACTCTGGAAAAGCAGCCCCCATTT CCTCGGGTACCAATAAATAAAACATGCAGGCTG

refseq_ADAMTS13.F5 (forward primer)
refseq_ADAMTS13.R5 (reverse primer)
Alternative donor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG LGCRLP*TATILEQGPACSRRSCRPPTLATAPA LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD GAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDP PRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKA VACTFAREHLDMCQALSCHTDPLDQSSCSRLLV PLLDGTECGVEKWCSKGRCRSLVELTPIAAVHG RWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFG GRACVGADLQAEMCNTQACEKTQLEFMSQQCAR TDGQPLRSSPGGASFYHWGAAVPHSQGDALCRH MCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTL SLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDN STCSPRKGSFTAGRAREYVTFLTVTPNLTSVYI ANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPS LLEDGRVEYRVALTEDRLPRLEEIRIWGPLQED ADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQA WVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELV ETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFG PCSASCGGGLRERPVRCVEAQGSLLKTLPPARC RAGAQQPAVALETCNPQPCPARWEVSEPSSCTSA GGAGLALENETCVPGADGLEAPVTEGPGSVDEK LPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWG SIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLC MDSALRVPVQEELCGLASKPGSRREVCQAVPCP ARWQYKLAACSVSCGRGVVRRILYCARAHGEDD GEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVM SLGPCSASCGLGTARRSVACVQLDQGQDVEVDE AACAALVRPEASVPCLIADCTYRWHVGTWMECS VSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKP VTVRGCWAGPCVGQGTPSLVPHEEAAAPGRTTA TPAGASLEWSQARGLLFSPAPQPRRLLPGPQEN SVQSSACGRQHLEPTGTIDMRGPGQADCAVAIGR PLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRK MCRKLLDMTFSSKTNTLVVRQRCGRPGGGVLLR YGSQLAPETFYRECDMQLFGPWGEIVSPSLSPAT SNAGGCRLFINVAPHARIAIHALATNMGAGTEG ANASYILIRDTHSLRTTAFHGQQVLYWESESSQ AEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQ SWKGKEGT*GSLNICSVSGQPWRVDPWSQCFPI RTFSNLRYLL*SLLQCPKG*GVGGGDSGKAAPI SSGTNK*NMQA

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC* ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT APRPAPASPGPPAAAGSC*ACSAQDGRAACGTR RGLNPGPRGTRRMRSLASTTAPTSSAAWPSAPR LSPAPSPGSTWICARPSPATQTRWTKAAAAASSF LSWMGQNVAWRSGAPRVAAAPWWS*PP*QQCMG AGLAGVPEVLAPAPAEEVWSPGGGSATTPDLPL GGVHVLVLTSRPRCATLRPARRPSWSSCRNSAPG PTASRCAPPLAAPPSTTGVLLYHTAKGMLCADT CAGPLARASS*SVETASSMGPGVCQVAPGRTGP *ACVCRAAAGHLAVMVGWTPSRYGTGARCVVGTT ARAAHGRALSQLAERENMSHF*QLPPT*PVSTL PTTGLSSHTWR*GSEGAMSWLGR*ASPLTPPTP PSWRMVVSSTEWPSPRTGCPAWRRSASGDPSRKM LTSRFTGGMARSMATSPAQTSPSPTSSLSHGRP GCGPLCVGPAR*AVGQGCAG*TTAAWTRPGRSW WRLSSAKGASSHQRGQRPACSNPALPTGRWETSA HAAPPVGAACGSGQCAAWRPRAAS*RHCPQPGA EQGPSSQLWRWKPATPSPALPGGRCQSPAHAHQ LVEQAWPWRTRPVCQGQMAWRLQ*LRGLAP*MRS CLPLSPVSGCHVLQAGAIWMPPLQGRRLPPHGA ASGRGLKLHTCGPLRQGRAPSPAGEV*WSCVSC AWTLPSGCLSRKSCVAWQASLGAGGRSARLSRAL LGGSTSWRPAA*AVGEGSCGGSCIVPGPMGRTM VRRSCWTPSARGCLARNPRRPAAWSPAHLGGKS CPLAHVRPAVALALLDARWPVCSSTKARTWRWTR RPVRRWCGPRPVSPVSLPTAPTAGMLAPGWSAL FPVGMASSAGVTPASDPRPRRLCQLISASTCPS R*LCVAAGLGPVWDRVRPAWCPTKKPLLQDGPQP PLLVPPWSGPRPGACSSPRLPSLGGSCPGPRKT QCSPVPVAGSTLSQQEPLTCEAQGRQTVQWPLG GPSGRW*PSASLRVLSTAVRGTCCCFGAGSPGGR CAGSCWT*LSAPRPTRWW*GSAAGGQEVGCCCG MGASLLLKPSTENVTCSSLGPGVKS*APR*VQP RVMQGAAGSSLMWLRTHGLPSMPWPPTWALGPRE PMPATS*SGTPTA*GPQRSMGSRCSTGSQRAAR LRWSSARAS*RLRPACGASTGPSNHGYRRCRTL SPGRERKEPEGH*TFVPCLASPGGLTPGLSAFQF ELFPILGIYFRVFSNVQKARGLEVGTLEKQPPF PRVPINKTCRL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA *W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG RRRAPRRPRLVPLQPPAAAEPAQRRTGALRVGPA AASTRVRGAPAGCAAWPLLQRQRAVPRGLRPQG CRLHLRQGAPGYVPGPLLPHRPAGPKQLQPPPR SSPGWDRMWRGEVVLQGSLPLPGGADPHSSSAWA LV*LGSPKSLLPLLRRRCGHQEAAVQQPQTCLW GACMCWC*PPGRDVQHSGLREDPAGVHVATVRQ DRRPAAALLPWRRLLLPLGCCCTTQPRGCSVQTH VPGHWRELHHEAWRQLPRWDPVYAKWPPGGRDP EPVCVGQLQDIWL*W*DGLPAGMGQVPGVWWGQ QHVQPTEGLFHSWQSERICHISDSYPQPDQCLHC QPQASLHTLGGEDRRALCRGWEDEHLP*HHLPL PPGGWSCRVQSGPHRGPAAPPGGDPHLGTPPGR C*HPGLQAVWRGVWQPHPPRHHLHLLPA*ATAGL GVGRCAWALLGELWGRAALGKLQLPGPGQEGVG GDCPVPREPAATSVARGLRARTLPSLLGGGRLR PMQRLLWGRPAGAASALRGGPGQPPEDIAPSPVQ SRGPAASCGAGNLQPPALPCQVGGVRAQLMHIS WWSRPGLGERDLCARGRWPGGSSD*GAWLRR*E AACP*ALCRDVMSSRLGPSGCHLCRGEGSLPMGQ HQDGGSSCTRVDPCGRVVLRLLRARSDGAAFPV HGLCPQGACPGRAVWPGKQAWEPAGGLPGCPVP CSVAVQAGGLQRELWERGRAEDPVLCPGPWGGRW *GDPVGHPVPGAASPGTPGGLQPGALPT*VESH VPWPMFGQLWPWHC*TLGGLCAARPRPGRGGGR GGLCGAGAARGQCPLSHCRLHLPLACWHLDGVLC FLWGWHPAPA*HLPRTPGPGACAS*FLPALAQA GDCAWLLGWALCGTGYAQPGAPRRSRCSRTDHS HPCWCLPGVVPGPGPALLPGSPASAAPARAPGKL SAVQCLWQAAP*ANRNH*HARPRAGRLCSGHWA APRGGGDPPRP*EFSQLQCGGHVAALGPAHLEE DVQEAVGHDFQLQDQHAGGEAALRAARRWGAAAV WEPACS*NLLQRM*HAALWALG*NREPLAESSH E*CRGLPALH*CGSARTDCHPCPGHQHGRWDRG SQCQLHLDPGHPQLEDHSVPWAAGALLGVREQPG *DGVQRGLPEGSGQPAGPVLDPPIMGTGDAGPS VLEGKGRNLRVIEHLFRVWPALEG*PLVSVLSN SNFFQS*VSTLESSPMSKRLGGWRWGLWKSSPHF LGYQ*IKHAG

Sequence NM_139026

Frame 0

Frame 1

Frame 2

Sequence NM_139027

Frame 0

Frame 1

Frame 2

Sequence NM_139025

Frame 0

Frame 1

Frame 2

Sequence NM_139028

Frame 0

Frame 1

Frame 2