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Sequences

Sequence NM_139027

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT
CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG
ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC
CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG
CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT
TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT
GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG
TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC
CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG
AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC
CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG
AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC
GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGCGCAGGACGGGCGCGCTGCGTGTGGGACCCGC
CGCGGCCTCAACCCGGGTCCGCGGGGCACCCGCCGGATGCGCAGCCTGGCCTCTACTACAGCGCCAACGAGCAGTGCCGCGTGGCCTTCGGCCCCAAGGC
TGTCGCCTGCACCTTCGCCAGGGAGCACCTGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAGCTGCAGCCGCCTCCTCGTT
CCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCCATAGCAGCAGTGCATGGGC
GCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACAACCCCAGACCTGCCTTTGG
GGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCATGTCGCAACAGTGCGCCAGG
ACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGGGATGCTCTGTGCAGACACA
TGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCCCCCGGGAGGACGGGACCCT
GAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCAGGTGTGTGGTGGGGACAAC
AGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAACCTGACCAGTGTCTACATTG
CCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCCCTAACACCACCTACCCCTC
CCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTGGGGACCCCTCCAGGAAGAT
GCTGACATCCAGGTTTACAGGCGGTATGGCGAGGAGTATGGCAACCTCACCCGCCCAGACATCACCTTCACCTACTTCCAGCCTAAGCCACGGCAGGCCT
GGGTGTGGGCCGCTGTGCGTGGGCCCTGCTCGGTGAGCTGTGGGGCAGGGCTGCGCTGGGTAAACTACAGCTGCCTGGACCAGGCCAGGAAGGAGTTGGT
GGAGACTGTCCAGTGCCAAGGGAGCCAGCAGCCACCAGCGTGGCCAGAGGCCTGCGTGCTCGAACCCTGCCCTCCCTACTGGGCGGTGGGAGACTTCGGC
CCATGCAGCGCCTCCTGTGGGGGCGGCCTGCGGGAGCGGCCAGTGCGCTGCGTGGAGGCCCAGGGCAGCCTCCTGAAGACATTGCCCCCAGCCCGGTGCA
GAGCAGGGGCCCAGCAGCCAGCTGTGGCGCTGGAAACCTGCAACCCCCAGCCCTGCCCTGCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCACATCAGC
TGGTGGAGCAGGCCTGGCCTTGGAGAACGAGACCTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGATGAGAAG
CTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCATGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCATGGGGCA
GCATCAGGACGGGGGCTCAAGCTGCACACGTGTGGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTTTCCTGTG
CATGGACTCTGCCCTCAGGGTGCCTGTCCAGGAAGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCCGTGCCCT
GCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGCGTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAGGACGATG
GTGAGGAGATCCTGTTGGACACCCAGTGCCAGGGGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTGCCCACCTAGGTGGAAAGTCAT
GTCCCTTGGCCCATGTTCGGCCAGCTGTGGCCTTGGCACTGCTAGACGCTCGGTGGCCTGTGTGCAGCTCGACCAAGGCCAGGACGTGGAGGTGGACGAG
GCGGCCTGTGCGGCGCTGGTGCGGCCCGAGGCCAGTGTCCCCTGTCTCATTGCCGACTGCACCTACCGCTGGCATGTTGGCACCTGGATGGAGTGCTCTG
TTTCCTGTGGGGATGGCATCCAGCGCCGGCGTGACACCTGCCTCGGACCCCAGGCCCAGGCGCCTGTGCCAGCTGATTTCTGCCAGCACTTGCCCAAGCC
GGTGACTGTGCGTGGCTGCTGGGCTGGGCCCTGTGTGGGACAGGGTGCCTGTGGCAGGCAGCACCTTGAGCCAACAGGAACCATTGACATGCGAGGCCCA
GGGCAGGCAGACTGTGCAGTGGCCATTGGGCGGCCCCTCGGGGAGGTGGTGACCCTCCGCGTCCTTGAGAGTTCTCTCAACTGCAGTGCGGGGGACATGT
TGCTGCTTTGGGGCCGGCTCACCTGGAGGAAGATGTGCAGGAAGCTGTTGGACATGACTTTCAGCTCCAAGACCAACACGCTGGTGGTGAGGCAGCGCTG
CGGGCGGCCAGGAGGTGGGGTGCTGCTGCGGTATGGGAGCCAGCTTGCTCCTGAAACCTTCTACAGAGAATGTGACATGCAGCTCTTTGGGCCCTGGGGT
GAAATCGTGAGCCCCTCGCTGAGTCCAGCCACGAGTAATGCAGGGGGCTGCCGGCTCTTCATTAATGTGGCTCCGCACGCACGGATTGCCATCCATGCCC
TGGCCACCAACATGGGCGCTGGGACCGAGGGAGCCAATGCCAGCTACATCTTGATCCGGGACACCCACAGCTTGAGGACCACAGCGTTCCATGGGCAGCA
GGTGCTCTACTGGGAGTCAGAGAGCAGCCAGGCTGAGATGGAGTTCAGCGAGGGCTTCCTGAAGGCTCAGGCCAGCCTGCGGGGCCAGTACTGGACCCTC
CAATCATGGGTACCGGAGATGCAGGACCCTCAGTCCTGGAAGGGAAAGGAAGGAACCTGAGGGTCATTGAACATTTGTTCCGTGTCTGGCCAGCCCTGGA
GGGTTGACCCCTGGTCTCAGTGCTTTCCAATTCGAACTTTTTCCAATCTTAGGTATCTACTTTAGAGTCTTCTCCAATGTCCAAAAGGCTAGGGGGTTGG
AGGTGGGGACTCTGGAAAAGCAGCCCCCATTTCCTCGGGTACCAATAAATAAAACATGCAGGCTG
  • refseq_ADAMTS13.F3 (forward primer)
  • refseq_ADAMTS13.R3 (reverse primer)
  • Alternative acceptor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP
SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG
LGCRLP*TATILEQGPACSRRSCRPPTLATAPA
LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK
APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL
ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP
GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL
VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS
LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL
SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL
PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG
VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD
GAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDP
PRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKA
VACTFAREHLDMCQALSCHTDPLDQSSCSRLLV
PLLDGTECGVEKWCSKGRCRSLVELTPIAAVHG
RWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFG
GRACVGADLQAEMCNTQACEKTQLEFMSQQCAR
TDGQPLRSSPGGASFYHWGAAVPHSQGDALCRH
MCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTL
SLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDN
STCSPRKGSFTAGRAREYVTFLTVTPNLTSVYI
ANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPS
LLEDGRVEYRVALTEDRLPRLEEIRIWGPLQED
ADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQA
WVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELV
ETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFG
PCSASCGGGLRERPVRCVEAQGSLLKTLPPARC
RAGAQQPAVALETCNPQPCPARWEVSEPSSCTSA
GGAGLALENETCVPGADGLEAPVTEGPGSVDEK
LPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWG
SIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLC
MDSALRVPVQEELCGLASKPGSRREVCQAVPCP
ARWQYKLAACSVSCGRGVVRRILYCARAHGEDD
GEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVM
SLGPCSASCGLGTARRSVACVQLDQGQDVEVDE
AACAALVRPEASVPCLIADCTYRWHVGTWMECS
VSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKP
VTVRGCWAGPCVGQGACGRQHLEPTGTIDMRGP
GQADCAVAIGRPLGEVVTLRVLESSLNCSAGDM
LLLWGRLTWRKMCRKLLDMTFSSKTNTLVVRQRC
GRPGGGVLLRYGSQLAPETFYRECDMQLFGPWG
EIVSPSLSPATSNAGGCRLFINVAPHARIAIHA
LATNMGAGTEGANASYILIRDTHSLRTTAFHGQQ
VLYWESESSQAEMEFSEGFLKAQASLRGQYWTL
QSWVPEMQDPQSWKGKEGT*GSLNICSVSGQPW
RVDPWSQCFPIRTFSNLRYLL*SLLQCPKG*GVG
GGDSGKAAPISSGTNK*NMQA

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR
LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ
DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP
WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR
PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES
LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL
VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW
WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR
PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC*
ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC
LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW
ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT
APRPAPASPGPPAAAGSC*ACSAQDGRAACGTR
RGLNPGPRGTRRMRSLASTTAPTSSAAWPSAPR
LSPAPSPGSTWICARPSPATQTRWTKAAAAASSF
LSWMGQNVAWRSGAPRVAAAPWWS*PP*QQCMG
AGLAGVPEVLAPAPAEEVWSPGGGSATTPDLPL
GGVHVLVLTSRPRCATLRPARRPSWSSCRNSAPG
PTASRCAPPLAAPPSTTGVLLYHTAKGMLCADT
CAGPLARASS*SVETASSMGPGVCQVAPGRTGP
*ACVCRAAAGHLAVMVGWTPSRYGTGARCVVGTT
ARAAHGRALSQLAERENMSHF*QLPPT*PVSTL
PTTGLSSHTWR*GSEGAMSWLGR*ASPLTPPTP
PSWRMVVSSTEWPSPRTGCPAWRRSASGDPSRKM
LTSRFTGGMARSMATSPAQTSPSPTSSLSHGRP
GCGPLCVGPAR*AVGQGCAG*TTAAWTRPGRSW
WRLSSAKGASSHQRGQRPACSNPALPTGRWETSA
HAAPPVGAACGSGQCAAWRPRAAS*RHCPQPGA
EQGPSSQLWRWKPATPSPALPGGRCQSPAHAHQ
LVEQAWPWRTRPVCQGQMAWRLQ*LRGLAP*MRS
CLPLSPVSGCHVLQAGAIWMPPLQGRRLPPHGA
ASGRGLKLHTCGPLRQGRAPSPAGEV*WSCVSC
AWTLPSGCLSRKSCVAWQASLGAGGRSARLSRAL
LGGSTSWRPAA*AVGEGSCGGSCIVPGPMGRTM
VRRSCWTPSARGCLARNPRRPAAWSPAHLGGKS
CPLAHVRPAVALALLDARWPVCSSTKARTWRWTR
RPVRRWCGPRPVSPVSLPTAPTAGMLAPGWSAL
FPVGMASSAGVTPASDPRPRRLCQLISASTCPS
R*LCVAAGLGPVWDRVPVAGSTLSQQEPLTCEAQ
GRQTVQWPLGGPSGRW*PSASLRVLSTAVRGTC
CCFGAGSPGGRCAGSCWT*LSAPRPTRWW*GSA
AGGQEVGCCCGMGASLLLKPSTENVTCSSLGPGV
KS*APR*VQPRVMQGAAGSSLMWLRTHGLPSMP
WPPTWALGPREPMPATS*SGTPTA*GPQRSMGS
RCSTGSQRAARLRWSSARAS*RLRPACGASTGPS
NHGYRRCRTLSPGRERKEPEGH*TFVPCLASPG
GLTPGLSAFQFELFPILGIYFRVFSNVQKARGL
EVGTLEKQPPFPRVPINKTCRL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV
CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR
TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC
PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG
PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP
CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP
WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG
GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV
PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA
ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA
*W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG
SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG
RRRAPRRPRLVPLQPPAAAEPAQRRTGALRVGPA
AASTRVRGAPAGCAAWPLLQRQRAVPRGLRPQG
CRLHLRQGAPGYVPGPLLPHRPAGPKQLQPPPR
SSPGWDRMWRGEVVLQGSLPLPGGADPHSSSAWA
LV*LGSPKSLLPLLRRRCGHQEAAVQQPQTCLW
GACMCWC*PPGRDVQHSGLREDPAGVHVATVRQ
DRRPAAALLPWRRLLLPLGCCCTTQPRGCSVQTH
VPGHWRELHHEAWRQLPRWDPVYAKWPPGGRDP
EPVCVGQLQDIWL*W*DGLPAGMGQVPGVWWGQ
QHVQPTEGLFHSWQSERICHISDSYPQPDQCLHC
QPQASLHTLGGEDRRALCRGWEDEHLP*HHLPL
PPGGWSCRVQSGPHRGPAAPPGGDPHLGTPPGR
C*HPGLQAVWRGVWQPHPPRHHLHLLPA*ATAGL
GVGRCAWALLGELWGRAALGKLQLPGPGQEGVG
GDCPVPREPAATSVARGLRARTLPSLLGGGRLR
PMQRLLWGRPAGAASALRGGPGQPPEDIAPSPVQ
SRGPAASCGAGNLQPPALPCQVGGVRAQLMHIS
WWSRPGLGERDLCARGRWPGGSSD*GAWLRR*E
AACP*ALCRDVMSSRLGPSGCHLCRGEGSLPMGQ
HQDGGSSCTRVDPCGRVVLRLLRARSDGAAFPV
HGLCPQGACPGRAVWPGKQAWEPAGGLPGCPVP
CSVAVQAGGLQRELWERGRAEDPVLCPGPWGGRW
*GDPVGHPVPGAASPGTPGGLQPGALPT*VESH
VPWPMFGQLWPWHC*TLGGLCAARPRPGRGGGR
GGLCGAGAARGQCPLSHCRLHLPLACWHLDGVLC
FLWGWHPAPA*HLPRTPGPGACAS*FLPALAQA
GDCAWLLGWALCGTGCLWQAAP*ANRNH*HARP
RAGRLCSGHWAAPRGGGDPPRP*EFSQLQCGGHV
AALGPAHLEEDVQEAVGHDFQLQDQHAGGEAAL
RAARRWGAAAVWEPACS*NLLQRM*HAALWALG
*NREPLAESSHE*CRGLPALH*CGSARTDCHPCP
GHQHGRWDRGSQCQLHLDPGHPQLEDHSVPWAA
GALLGVREQPG*DGVQRGLPEGSGQPAGPVLDP
PIMGTGDAGPSVLEGKGRNLRVIEHLFRVWPALE
G*PLVSVLSNSNFFQS*VSTLESSPMSKRLGGW
RWGLWKSSPHFLGYQ*IKHAG

Sequence NM_139025

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT
CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG
ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC
CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG
CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT
TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT
GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG
TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC
CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG
AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC
CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG
AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC
GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGCGCAGGACGGGCGCGCTGCGTGTGGGACCCGC
CGCGGCCTCAACCCGGGTCCGCGGGGCACCCGCCGGATGCGCAGCCTGGCCTCTACTACAGCGCCAACGAGCAGTGCCGCGTGGCCTTCGGCCCCAAGGC
TGTCGCCTGCACCTTCGCCAGGGAGCACCTGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAGCTGCAGCCGCCTCCTCGTT
CCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCCATAGCAGCAGTGCATGGGC
GCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACAACCCCAGACCTGCCTTTGG
GGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCATGTCGCAACAGTGCGCCAGG
ACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGGGATGCTCTGTGCAGACACA
TGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCCCCCGGGAGGACGGGACCCT
GAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCAGGTGTGTGGTGGGGACAAC
AGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAACCTGACCAGTGTCTACATTG
CCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCCCTAACACCACCTACCCCTC
CCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTGGGGACCCCTCCAGGAAGAT
GCTGACATCCAGGTTTACAGGCGGTATGGCGAGGAGTATGGCAACCTCACCCGCCCAGACATCACCTTCACCTACTTCCAGCCTAAGCCACGGCAGGCCT
GGGTGTGGGCCGCTGTGCGTGGGCCCTGCTCGGTGAGCTGTGGGGCAGGGCTGCGCTGGGTAAACTACAGCTGCCTGGACCAGGCCAGGAAGGAGTTGGT
GGAGACTGTCCAGTGCCAAGGGAGCCAGCAGCCACCAGCGTGGCCAGAGGCCTGCGTGCTCGAACCCTGCCCTCCCTACTGGGCGGTGGGAGACTTCGGC
CCATGCAGCGCCTCCTGTGGGGGCGGCCTGCGGGAGCGGCCAGTGCGCTGCGTGGAGGCCCAGGGCAGCCTCCTGAAGACATTGCCCCCAGCCCGGTGCA
GAGCAGGGGCCCAGCAGCCAGCTGTGGCGCTGGAAACCTGCAACCCCCAGCCCTGCCCTGCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCACATCAGC
TGGTGGAGCAGGCCTGGCCTTGGAGAACGAGACCTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGATGAGAAG
CTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCATGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCATGGGGCA
GCATCAGGACGGGGGCTCAAGCTGCACACGTGTGGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTTTCCTGTG
CATGGACTCTGCCCTCAGGGTGCCTGTCCAGGAAGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCCGTGCCCT
GCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGCGTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAGGACGATG
GTGAGGAGATCCTGTTGGACACCCAGTGCCAGGGGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTGCCCACCTAGGTGGAAAGTCAT
GTCCCTTGGCCCATGTTCGGCCAGCTGTGGCCTTGGCACTGCTAGACGCTCGGTGGCCTGTGTGCAGCTCGACCAAGGCCAGGACGTGGAGGTGGACGAG
GCGGCCTGTGCGGCGCTGGTGCGGCCCGAGGCCAGTGTCCCCTGTCTCATTGCCGACTGCACCTACCGCTGGCATGTTGGCACCTGGATGGAGTGCTCTG
TTTCCTGTGGGGATGGCATCCAGCGCCGGCGTGACACCTGCCTCGGACCCCAGGCCCAGGCGCCTGTGCCAGCTGATTTCTGCCAGCACTTGCCCAAGCC
GGTGACTGTGCGTGGCTGCTGGGCTGGGCCCTGTGTGGGACAGGGTACGCCCAGCCTGGTGCCCCACGAAGAAGCCGCTGCTCCAGGACGGACCACAGCC
ACCCCTGCTGGTGCCTCCCTGGAGTGGTCCCAGGCCCGGGGCCTGCTCTTCTCCCCGGCTCCCCAGCCTCGGCGGCTCCTGCCCGGGCCCCAGGAAAACT
CAGTGCAGTCCAGTGCCTGTGGCAGGCAGCACCTTGAGCCAACAGGAACCATTGACATGCGAGGCCCAGGGCAGGCAGACTGTGCAGTGGCCATTGGGCG
GCCCCTCGGGGAGGTGGTGACCCTCCGCGTCCTTGAGAGTTCTCTCAACTGCAGTGCGGGGGACATGTTGCTGCTTTGGGGCCGGCTCACCTGGAGGAAG
ATGTGCAGGAAGCTGTTGGACATGACTTTCAGCTCCAAGACCAACACGCTGGTGGTGAGGCAGCGCTGCGGGCGGCCAGGAGGTGGGGTGCTGCTGCGGT
ATGGGAGCCAGCTTGCTCCTGAAACCTTCTACAGAGAATGTGACATGCAGCTCTTTGGGCCCTGGGGTGAAATCGTGAGCCCCTCGCTGAGTCCAGCCAC
GAGTAATGCAGGGGGCTGCCGGCTCTTCATTAATGTGGCTCCGCACGCACGGATTGCCATCCATGCCCTGGCCACCAACATGGGCGCTGGGACCGAGGGA
GCCAATGCCAGCTACATCTTGATCCGGGACACCCACAGCTTGAGGACCACAGCGTTCCATGGGCAGCAGGTGCTCTACTGGGAGTCAGAGAGCAGCCAGG
CTGAGATGGAGTTCAGCGAGGGCTTCCTGAAGGCTCAGGCCAGCCTGCGGGGCCAGTACTGGACCCTCCAATCATGGGTACCGGAGATGCAGGACCCTCA
GTCCTGGAAGGGAAAGGAAGGAACCTGAGGGTCATTGAACATTTGTTCCGTGTCTGGCCAGCCCTGGAGGGTTGACCCCTGGTCTCAGTGCTTTCCAATT
CGAACTTTTTCCAATCTTAGGTATCTACTTTAGAGTCTTCTCCAATGTCCAAAAGGCTAGGGGGTTGGAGGTGGGGACTCTGGAAAAGCAGCCCCCATTT
CCTCGGGTACCAATAAATAAAACATGCAGGCTG
  • refseq_ADAMTS13.F3 (forward primer)
  • refseq_ADAMTS13.R3 (reverse primer)
  • Alternative acceptor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP
SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG
LGCRLP*TATILEQGPACSRRSCRPPTLATAPA
LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK
APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL
ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP
GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL
VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS
LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL
SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL
PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG
VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD
GAAPRAGLAWSPCSRRQLLSLLSAGRARCVWDP
PRPQPGSAGHPPDAQPGLYYSANEQCRVAFGPKA
VACTFAREHLDMCQALSCHTDPLDQSSCSRLLV
PLLDGTECGVEKWCSKGRCRSLVELTPIAAVHG
RWSSWGPRSPCSRSCGGGVVTRRRQCNNPRPAFG
GRACVGADLQAEMCNTQACEKTQLEFMSQQCAR
TDGQPLRSSPGGASFYHWGAAVPHSQGDALCRH
MCRAIGESFIMKRGDSFLDGTRCMPSGPREDGTL
SLCVSGSCRTFGCDGRMDSQQVWDRCQVCGGDN
STCSPRKGSFTAGRAREYVTFLTVTPNLTSVYI
ANHRPLFTHLAVRIGGRYVVAGKMSISPNTTYPS
LLEDGRVEYRVALTEDRLPRLEEIRIWGPLQED
ADIQVYRRYGEEYGNLTRPDITFTYFQPKPRQA
WVWAAVRGPCSVSCGAGLRWVNYSCLDQARKELV
ETVQCQGSQQPPAWPEACVLEPCPPYWAVGDFG
PCSASCGGGLRERPVRCVEAQGSLLKTLPPARC
RAGAQQPAVALETCNPQPCPARWEVSEPSSCTSA
GGAGLALENETCVPGADGLEAPVTEGPGSVDEK
LPAPEPCVGMSCPPGWGHLDATSAGEKAPSPWG
SIRTGAQAAHVWTPAAGSCSVSCGRGLMELRFLC
MDSALRVPVQEELCGLASKPGSRREVCQAVPCP
ARWQYKLAACSVSCGRGVVRRILYCARAHGEDD
GEEILLDTQCQGLPRPEPQEACSLEPCPPRWKVM
SLGPCSASCGLGTARRSVACVQLDQGQDVEVDE
AACAALVRPEASVPCLIADCTYRWHVGTWMECS
VSCGDGIQRRRDTCLGPQAQAPVPADFCQHLPKP
VTVRGCWAGPCVGQGTPSLVPHEEAAAPGRTTA
TPAGASLEWSQARGLLFSPAPQPRRLLPGPQEN
SVQSSACGRQHLEPTGTIDMRGPGQADCAVAIGR
PLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRK
MCRKLLDMTFSSKTNTLVVRQRCGRPGGGVLLR
YGSQLAPETFYRECDMQLFGPWGEIVSPSLSPAT
SNAGGCRLFINVAPHARIAIHALATNMGAGTEG
ANASYILIRDTHSLRTTAFHGQQVLYWESESSQ
AEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQ
SWKGKEGT*GSLNICSVSGQPWRVDPWSQCFPI
RTFSNLRYLL*SLLQCPKG*GVGGGDSGKAAPI
SSGTNK*NMQA

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR
LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ
DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP
WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR
PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES
LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL
VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW
WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR
PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC*
ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC
LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW
ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT
APRPAPASPGPPAAAGSC*ACSAQDGRAACGTR
RGLNPGPRGTRRMRSLASTTAPTSSAAWPSAPR
LSPAPSPGSTWICARPSPATQTRWTKAAAAASSF
LSWMGQNVAWRSGAPRVAAAPWWS*PP*QQCMG
AGLAGVPEVLAPAPAEEVWSPGGGSATTPDLPL
GGVHVLVLTSRPRCATLRPARRPSWSSCRNSAPG
PTASRCAPPLAAPPSTTGVLLYHTAKGMLCADT
CAGPLARASS*SVETASSMGPGVCQVAPGRTGP
*ACVCRAAAGHLAVMVGWTPSRYGTGARCVVGTT
ARAAHGRALSQLAERENMSHF*QLPPT*PVSTL
PTTGLSSHTWR*GSEGAMSWLGR*ASPLTPPTP
PSWRMVVSSTEWPSPRTGCPAWRRSASGDPSRKM
LTSRFTGGMARSMATSPAQTSPSPTSSLSHGRP
GCGPLCVGPAR*AVGQGCAG*TTAAWTRPGRSW
WRLSSAKGASSHQRGQRPACSNPALPTGRWETSA
HAAPPVGAACGSGQCAAWRPRAAS*RHCPQPGA
EQGPSSQLWRWKPATPSPALPGGRCQSPAHAHQ
LVEQAWPWRTRPVCQGQMAWRLQ*LRGLAP*MRS
CLPLSPVSGCHVLQAGAIWMPPLQGRRLPPHGA
ASGRGLKLHTCGPLRQGRAPSPAGEV*WSCVSC
AWTLPSGCLSRKSCVAWQASLGAGGRSARLSRAL
LGGSTSWRPAA*AVGEGSCGGSCIVPGPMGRTM
VRRSCWTPSARGCLARNPRRPAAWSPAHLGGKS
CPLAHVRPAVALALLDARWPVCSSTKARTWRWTR
RPVRRWCGPRPVSPVSLPTAPTAGMLAPGWSAL
FPVGMASSAGVTPASDPRPRRLCQLISASTCPS
R*LCVAAGLGPVWDRVRPAWCPTKKPLLQDGPQP
PLLVPPWSGPRPGACSSPRLPSLGGSCPGPRKT
QCSPVPVAGSTLSQQEPLTCEAQGRQTVQWPLG
GPSGRW*PSASLRVLSTAVRGTCCCFGAGSPGGR
CAGSCWT*LSAPRPTRWW*GSAAGGQEVGCCCG
MGASLLLKPSTENVTCSSLGPGVKS*APR*VQP
RVMQGAAGSSLMWLRTHGLPSMPWPPTWALGPRE
PMPATS*SGTPTA*GPQRSMGSRCSTGSQRAAR
LRWSSARAS*RLRPACGASTGPSNHGYRRCRTL
SPGRERKEPEGH*TFVPCLASPGGLTPGLSAFQF
ELFPILGIYFRVFSNVQKARGLEVGTLEKQPPF
PRVPINKTCRL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV
CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR
TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC
PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG
PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP
CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP
WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG
GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV
PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA
ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA
*W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG
SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG
RRRAPRRPRLVPLQPPAAAEPAQRRTGALRVGPA
AASTRVRGAPAGCAAWPLLQRQRAVPRGLRPQG
CRLHLRQGAPGYVPGPLLPHRPAGPKQLQPPPR
SSPGWDRMWRGEVVLQGSLPLPGGADPHSSSAWA
LV*LGSPKSLLPLLRRRCGHQEAAVQQPQTCLW
GACMCWC*PPGRDVQHSGLREDPAGVHVATVRQ
DRRPAAALLPWRRLLLPLGCCCTTQPRGCSVQTH
VPGHWRELHHEAWRQLPRWDPVYAKWPPGGRDP
EPVCVGQLQDIWL*W*DGLPAGMGQVPGVWWGQ
QHVQPTEGLFHSWQSERICHISDSYPQPDQCLHC
QPQASLHTLGGEDRRALCRGWEDEHLP*HHLPL
PPGGWSCRVQSGPHRGPAAPPGGDPHLGTPPGR
C*HPGLQAVWRGVWQPHPPRHHLHLLPA*ATAGL
GVGRCAWALLGELWGRAALGKLQLPGPGQEGVG
GDCPVPREPAATSVARGLRARTLPSLLGGGRLR
PMQRLLWGRPAGAASALRGGPGQPPEDIAPSPVQ
SRGPAASCGAGNLQPPALPCQVGGVRAQLMHIS
WWSRPGLGERDLCARGRWPGGSSD*GAWLRR*E
AACP*ALCRDVMSSRLGPSGCHLCRGEGSLPMGQ
HQDGGSSCTRVDPCGRVVLRLLRARSDGAAFPV
HGLCPQGACPGRAVWPGKQAWEPAGGLPGCPVP
CSVAVQAGGLQRELWERGRAEDPVLCPGPWGGRW
*GDPVGHPVPGAASPGTPGGLQPGALPT*VESH
VPWPMFGQLWPWHC*TLGGLCAARPRPGRGGGR
GGLCGAGAARGQCPLSHCRLHLPLACWHLDGVLC
FLWGWHPAPA*HLPRTPGPGACAS*FLPALAQA
GDCAWLLGWALCGTGYAQPGAPRRSRCSRTDHS
HPCWCLPGVVPGPGPALLPGSPASAAPARAPGKL
SAVQCLWQAAP*ANRNH*HARPRAGRLCSGHWA
APRGGGDPPRP*EFSQLQCGGHVAALGPAHLEE
DVQEAVGHDFQLQDQHAGGEAALRAARRWGAAAV
WEPACS*NLLQRM*HAALWALG*NREPLAESSH
E*CRGLPALH*CGSARTDCHPCPGHQHGRWDRG
SQCQLHLDPGHPQLEDHSVPWAAGALLGVREQPG
*DGVQRGLPEGSGQPAGPVLDPPIMGTGDAGPS
VLEGKGRNLRVIEHLFRVWPALEG*PLVSVLSN
SNFFQS*VSTLESSPMSKRLGGWRWGLWKSSPHF
LGYQ*IKHAG

Sequence NM_139028

AACCACGATGTCTTTGGCACAGCCTCTCATCTGTCAGATGGGAGCGGGGACCCCGGAGAGGGAGTCAGCCGAGGTCCTGGCATTCCTTGTGAACCCCCGT
CTGTGGGTTTCTGGTCCAGTGTCCCTTCTCCAGATTAGATGGCTTAGGCCTCCTCTAAGGGGGTGGGCGTGCACATCCGGAGAGCTGTCTGGTGTGCAGG
ACTGGGCTGCAGGTTACCCTGAACTGCAACCATCTTAGAGCAAGGCCCAGCTTGCAGCAGGAGGAGCTGCAGGCCGCCCACCCTAGCCACGGCCCCTGCC
CTGGCAGGAAGCTTCCAAGAGTAAACACTGCCTAATCGTCCCGCCCAGTAGTGAGCAGGCCTGTCCCATTCCATACTGACCAGATTCCCAGTCACCAAGG
CCCCCTCTCACTCCGCTCCACTCCTCGGGCTGGCTCTCCTGAGGATGCACCAGCGTCACCCCCGGGCAAGATGCCCTCCCCTCTGTGTGGCCGGAATCCT
TGCCTGTGGCTTTCTCCTGGGCTGCTGGGGACCCTCCCATTTCCAGCAGAGTTGTCTTCAGGCTTTGGAGCCACAGGCCGTGTCTTCTTACTTGAGCCCT
GGTGCTCCCTTAAAAGGCCGCCCTCCTTCCCCTGGCTTCCAGAGGCAGAGGCAGAGGCAGAGGCGGGCTGCAGGCGGCATCCTACACCTGGAGCTGCTGG
TGGCCGTGGGCCCCGATGTCTTCCAGGCTCACCAGGAGGACACAGAGCGCTATGTGCTCACCAACCTCAACATCGGGGCAGAACTGCTTCGGGACCCGTC
CCTGGGGGCTCAGTTTCGGGTGCACCTGGTGAAGATGGTCATTCTGACAGAGCCTGAGGGTGCCCCAAATATCACAGCCAACCTCACCTCGTCCCTGCTG
AGCGTCTGTGGGTGGAGCCAGACCATCAACCCTGAGGACGACACGGATCCTGGCCATGCTGACCTGGTCCTCTATATCACTAGGTTTGACCTGGAGTTGC
CTGATGGTAACCGGCAGGTGCGGGGCGTCACCCAGCTGGGCGGTGCCTGCTCCCCAACCTGGAGCTGCCTCATTACCGAGGACACTGGCTTCGACCTGGG
AGTCACCATTGCCCATGAGATTGGGCACAGCTTCGGCCTGGAGCACGACGGCGCGCCCGGCAGCGGCTGCGGCCCCAGCGGACACGTGATGGCTTCGGAC
GGCGCCGCGCCCCGCGCCGGCCTCGCCTGGTCCCCCTGCAGCCGCCGGCAGCTGCTGAGCCTGCTCAGACCCGTCCCTCCGTCGCCGCTCCCTCTGCTGG
CCACCCACCTCTGCGCCGGCAGGAGCCTTAGTCTTGGTCCCAGCCAAGAGCCGGCTCCTGGTGGGGGGCGCGGGCCGAGAACTCCTGTTCCCACTCACAA
AAGGCCACGCTTCCAAACGCTTCCATCCTCGTGCCCACTCCTCCGTCCCGCCTCCTCCCGGTGTACACCCCGGGACTGAGCCGGGCCTGAGCCGGGCCTT
GTCGCAG
CGCAGGACGGGCGCGCTGCGTGTGGGACCCGCCGCGGCCTCAACCCGGGTCCGCGGGGCACCCGCCGGATGCGCAGCCTGGCCTCTACTACAG
CGCCAACGAGCAG
TGCCGCGTGGCCTTCGGCCCCAAGGCTGTCGCCTGCACCTTCGCCAGGGAGCACCTGGTGAGTCTGCCGGCGGTGGCCTGGGATTGG
CTGTGAGGTCCCTCCGCATCACCCAGCTCACGTCCCCCAAAACGTGCATGGATATGTGCCAGGCCCTCTCCTGCCACACAGACCCGCTGGACCAAAGCAG
CTGCAGCCGCCTCCTCGTTCCTCTCCTGGATGGGACAGAATGTGGCGTGGAGAAGTGGTGCTCCAAGGGTCGCTGCCGCTCCCTGGTGGAGCTGACCCCC
ATAGCAGCAGTGCATGGGCGCTGGTCTAGCTGGGGTCCCCGAAGTCCTTGCTCCCGCTCCTGCGGAGGAGGTGTGGTCACCAGGAGGCGGCAGTGCAACA
ACCCCAGACCTGCCTTTGGGGGGCGTGCATGTGTTGGTGCTGACCTCCAGGCCGAGATGTGCAACACTCAGGCCTGCGAGAAGACCCAGCTGGAGTTCAT
GTCGCAACAGTGCGCCAGGACCGACGGCCAGCCGCTGCGCTCCTCCCCTGGCGGCGCCTCCTTCTACCACTGGGGTGCTGCTGTACCACACAGCCAAGGG
GATGCTCTGTGCAGACACATGTGCCGGGCCATTGGCGAGAGCTTCATCATGAAGCGTGGAGACAGCTTCCTCGATGGGACCCGGTGTATGCCAAGTGGCC
CCCGGGAGGACGGGACCCTGAGCCTGTGTGTGTCGGGCAGCTGCAGGACATTTGGCTGTGATGGTAGGATGGACTCCCAGCAGGTATGGGACAGGTGCCA
GGTGTGTGGTGGGGACAACAGCACGTGCAGCCCACGGAAGGGCTCTTTCACAGCTGGCAGAGCGAGAGAATATGTCACATTTCTGACAGTTACCCCCAAC
CTGACCAGTGTCTACATTGCCAACCACAGGCCTCTCTTCACACACTTGGCGGTGAGGATCGGAGGGCGCTATGTCGTGGCTGGGAAGATGAGCATCTCCC
CTAACACCACCTACCCCTCCCTCCTGGAGGATGGTCGTGTCGAGTACAGAGTGGCCCTCACCGAGGACCGGCTGCCCCGCCTGGAGGAGATCCGCATCTG
GGGACCCCTCCAGGAAGATGCTGACATCCAGGTGGGAGGTGTCAGAGCCCAGCTCATGCACATCAGCTGGTGGAGCAGGCCTGGCCTTGGAGAACGAGAC
CTGTGTGCCAGGGGCAGATGGCCTGGAGGCTCCAGTGACTGAGGGGCCTGGCTCCGTAGATGAGAAGCTGCCTGCCCCTGAGCCCTGTGTCGGGATGTCA
TGTCCTCCAGGCTGGGGCCATCTGGATGCCACCTCTGCAGGGGAGAAGGCTCCCTCCCCATGGGGCAGCATCAGGACGGGGGCTCAAGCTGCACACGTGT
GGACCCCTGCGGCAGGGTCGTGCTCCGTCTCCTGCGGGCGAGGTCTGATGGAGCTGCGTTTCCTGTGCATGGACTCTGCCCTCAGGGTGCCTGTCCAGGA
AGAGCTGTGTGGCCTGGCAAGCAAGCCTGGGAGCCGGCGGGAGGTCTGCCAGGCTGTCCCGTGCCCTGCTCGGTGGCAGTACAAGCTGGCGGCCTGCAGC
GTGAGCTGTGGGAGAGGGGTCGTGCGGAGGATCCTGTATTGTGCCCGGGCCCATGGGGAGGACGATGGTGAGGAGATCCTGTTGGACACCCAGTGCCAGG
GGCTGCCTCGCCCGGAACCCCAGGAGGCCTGCAGCCTGGAGCCCTG
  • refseq_ADAMTS13.F3 (forward primer)
  • refseq_ADAMTS13.R3 (reverse primer)
  • Alternative acceptor

Frame 0

NHDVFGTASHLSDGSGDPGEGVSRGPGIPCEPP
SVGFWSSVPSPD*MA*ASSKGVGVHIRRAVWCAG
LGCRLP*TATILEQGPACSRRSCRPPTLATAPA
LAGSFQE*TLPNRPAQ**AGLSHSILTRFPVTK
APSHSAPLLGLALLRMHQRHPRARCPPLCVAGIL
ACGFLLGCWGPSHFQQSCLQALEPQAVSSYLSP
GAPLKGRPPSPGFQRQRQRQRRAAGGILHLELL
VAVGPDVFQAHQEDTERYVLTNLNIGAELLRDPS
LGAQFRVHLVKMVILTEPEGAPNITANLTSSLL
SVCGWSQTINPEDDTDPGHADLVLYITRFDLEL
PDGNRQVRGVTQLGGACSPTWSCLITEDTGFDLG
VTIAHEIGHSFGLEHDGAPGSGCGPSGHVMASD
GAAPRAGLAWSPCSRRQLLSLLRPVPPSPLPLL
ATHLCAGRSLSLGPSQEPAPGGGRGPRTPVPTHK
RPRFQTLPSSCPLLRPASSRCTPRD*AGPEPGL
VA
AQDGRAACGTRRGLNPGPRGTRRMRSLASTT
APTSS
AAWPSAPRLSPAPSPGSTW*VCRRWPGIG
CEVPPHHPAHVPQNVHGYVPGPLLPHRPAGPKQ
LQPPPRSSPGWDRMWRGEVVLQGSLPLPGGADP
HSSSAWALV*LGSPKSLLPLLRRRCGHQEAAVQQ
PQTCLWGACMCWC*PPGRDVQHSGLREDPAGVH
VATVRQDRRPAAALLPWRRLLLPLGCCCTTQPR
GCSVQTHVPGHWRELHHEAWRQLPRWDPVYAKWP
PGGRDPEPVCVGQLQDIWL*W*DGLPAGMGQVP
GVWWGQQHVQPTEGLFHSWQSERICHISDSYPQ
PDQCLHCQPQASLHTLGGEDRRALCRGWEDEHLP
*HHLPLPPGGWSCRVQSGPHRGPAAPPGGDPHL
GTPPGRC*HPGGRCQSPAHAHQLVEQAWPWRTR
PVCQGQMAWRLQ*LRGLAP*MRSCLPLSPVSGCH
VLQAGAIWMPPLQGRRLPPHGAASGRGLKLHTC
GPLRQGRAPSPAGEV*WSCVSCAWTLPSGCLSR
KSCVAWQASLGAGGRSARLSRALLGGSTSWRPAA
*AVGEGSCGGSCIVPGPMGRTMVRRSCWTPSAR
GCLARNPRRPAAWSP

Frame 1

TTMSLAQPLICQMGAGTPERESAEVLAFLVNPR
LWVSGPVSLLQIRWLRPPLRGWACTSGELSGVQ
DWAAGYPELQPS*SKAQLAAGGAAGRPP*PRPLP
WQEASKSKHCLIVPPSSEQACPIPY*PDSQSPR
PPLTPLHSSGWLS*GCTSVTPGQDALPSVWPES
LPVAFSWAAGDPPISSRVVFRLWSHRPCLLT*AL
VLP*KAALLPLASRGRGRGRGGLQAASYTWSCW
WPWAPMSSRLTRRTQSAMCSPTSTSGQNCFGTR
PWGLSFGCTW*RWSF*QSLRVPQISQPTSPRPC*
ASVGGARPSTLRTTRILAMLTWSSISLGLTWSC
LMVTGRCGASPSWAVPAPQPGAASLPRTLASTW
ESPLPMRLGTASAWSTTARPAAAAAPADT*WLRT
APRPAPASPGPPAAAGSC*ACSDPSLRRRSLCW
PPTSAPAGALVLVPAKSRLLVGGAGRELLFPLT
KGHASKRFHPRAHSSVPPPPGVHPGTEPGLSRAL
SQ
RRTGALRVGPAAASTRVRGAPAGCAAWPLLQ
RQRA
VPRGLRPQGCRLHLRQGAPGESAGGGLGL
AVRSLRITQLTSPKTCMDMCQALSCHTDPLDQSS
CSRLLVPLLDGTECGVEKWCSKGRCRSLVELTP
IAAVHGRWSSWGPRSPCSRSCGGGVVTRRRQCN
NPRPAFGGRACVGADLQAEMCNTQACEKTQLEFM
SQQCARTDGQPLRSSPGGASFYHWGAAVPHSQG
DALCRHMCRAIGESFIMKRGDSFLDGTRCMPSG
PREDGTLSLCVSGSCRTFGCDGRMDSQQVWDRCQ
VCGGDNSTCSPRKGSFTAGRAREYVTFLTVTPN
LTSVYIANHRPLFTHLAVRIGGRYVVAGKMSIS
PNTTYPSLLEDGRVEYRVALTEDRLPRLEEIRIW
GPLQEDADIQVGGVRAQLMHISWWSRPGLGERD
LCARGRWPGGSSD*GAWLRR*EAACP*ALCRDV
MSSRLGPSGCHLCRGEGSLPMGQHQDGGSSCTRV
DPCGRVVLRLLRARSDGAAFPVHGLCPQGACPG
RAVWPGKQAWEPAGGLPGCPVPCSVAVQAGGLQ
RELWERGRAEDPVLCPGPWGGRW*GDPVGHPVPG
AASPGTPGGLQPGAL

Frame 2

PRCLWHSLSSVRWERGPRRGSQPRSWHSL*TPV
CGFLVQCPFSRLDGLGLL*GGGRAHPESCLVCR
TGLQVTLNCNHLRARPSLQQEELQAAHPSHGPC
PGRKLPRVNTA*SSRPVVSRPVPFHTDQIPSHQG
PLSLRSTPRAGSPEDAPASPPGKMPSPLCGRNP
CLWLSPGLLGTLPFPAELSSGFGATGRVFLLEP
WCSLKRPPSFPWLPEAEAEAEAGCRRHPTPGAAG
GRGPRCLPGSPGGHRALCAHQPQHRGRTASGPV
PGGSVSGAPGEDGHSDRA*GCPKYHSQPHLVPA
ERLWVEPDHQP*GRHGSWPC*PGPLYH*V*PGVA
*W*PAGAGRHPAGRCLLPNLELPHYRGHWLRPG
SHHCP*DWAQLRPGARRRARQRLRPQRTRDGFG
RRRAPRRPRLVPLQPPAAAEPAQTRPSVAAPSAG
HPPLRRQEP*SWSQPRAGSWWGARAENSCSHSQ
KATLPNASILVPTPPSRLLPVYTPGLSRA*AGP
CRS
AGRARCVWDPPRPQPGSAGHPPDAQPGLYYS
ANEQ
CRVAFGPKAVACTFAREHLVSLPAVAWDW
L*GPSASPSSRPPKRAWICARPSPATQTRWTKA
AAAASSFLSWMGQNVAWRSGAPRVAAAPWWS*PP
*QQCMGAGLAGVPEVLAPAPAEEVWSPGGGSAT
TPDLPLGGVHVLVLTSRPRCATLRPARRPSWSS
CRNSAPGPTASRCAPPLAAPPSTTGVLLYHTAKG
MLCADTCAGPLARASS*SVETASSMGPGVCQVA
PGRTGP*ACVCRAAAGHLAVMVGWTPSRYGTGA
RCVVGTTARAAHGRALSQLAERENMSHF*QLPPT
*PVSTLPTTGLSSHTWR*GSEGAMSWLGR*ASP
LTPPTPPSWRMVVSSTEWPSPRTGCPAWRRSAS
GDPSRKMLTSRWEVSEPSSCTSAGGAGLALENET
CVPGADGLEAPVTEGPGSVDEKLPAPEPCVGMS
CPPGWGHLDATSAGEKAPSPWGSIRTGAQAAHV
WTPAAGSCSVSCGRGLMELRFLCMDSALRVPVQE
ELCGLASKPGSRREVCQAVPCPARWQYKLAACS
VSCGRGVVRRILYCARAHGEDDGEEILLDTQCQ
GLPRPEPQEACSLEP

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