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Sequence NM_080648
CAGACAGACCAATCACGCGCATTCTTCGGCCACGACAAGCGCGCCTCTGATCACGTGACCAGGTCCGCTACCCACGTGGGGGCTCAGCGTGCACCCTTCTTTGTGCTCGGGTTAGGAGGAGCTAGGCTGCCATCGGGCCGGTGCAGATACGGGGTTGCTCTTTTGCTCATAAGAGGGGCTTCGCTGGCAGTCTGAACGGC
AACGCGGTAAAAATATTGCTTCGGTGGGTGACGCGGTACAGCTGCCCAAGGGCGTTCGTAACGGGAATGCCGAAGCGTGGGAAAAAGGGAGCGGTGGCGG
AAGACGGGGATGAGCTCAGGACAGAGCCAGAGGCCAAGAAGAGTAAGACGGCCGCAAAGAAAAATGACAAAGAGGCAGCAGGAGAGGGCCCAGCCCTGTA
TGAGGACCCCCCAGATCAGAAAACCTCACCCAGTGGCAAACCTGCCACACTCAAGATCTGCTCTTGGAATGTGGATGGGCTTCGAGCCTGGATTAAGAAG
AAAGGATTAGATTGGGTAAAGGAAGAAGCCCCAGATATACTGTGCCTTCAAGAGACCAAATGTTCAGAGAACAAACTACCAGCTGAACTTCAGGAGCTGC
CTGGACTCTCTCATCAATACTGGTCAGCTCCTTCGGACAAGGAAGGGTACAGTGGCGTGGGCCTGCTTTCCCGCCAGTGCCCACTCAAAGTTTCTTACGG
CATAGGCGATGAGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGACTCGTTTGTGCTGGTAACAGCATATGTACCTAATGCAGGCCGAGGT
CTGGTACGACTGGAGTACCGGCAGCGCTGGGATGAAGCCTTTCGCAAGTTCCTGAAGGGCCTGGCTTCCCGAAAGCCCCTTGTGCTGTGTGGAGACCTCA
ATGTGGCACATGAAGAAATTGACCTTCGCAACCCCAAGGGGAACAAAAAGAATGCTGGCTTCACGCCACAAGAGCGCCAAGGCTTCGGGGAATTACTGCA
GGCTGTGCCACTGGCTGACAGCTTTAGGCACCTCTACCCCAACACACCCTATGCCTACACCTTTTGGACTTATATGATGAATGCTCGATCCAAGAATGTT
GGTTGGCGCCTTGATTACTTTTTGTTGTCCCACTCTCTGTTACCTGCATTGTGTGACAGCAAGATCCGTTCCAAGGCCCTCGGCAGTGATCACTGTCCTA
TCACCCTATACCTAGCACTGTGACACCACCCCTAAATCACTTTGAGCCTGGGAAATAAGCCCCCTCAACTACCATTCCTTCTTTAAACACTCTTCAGAGA
AATCTGCATTCTATTTCTCATGTATAAAACTAGGAATCCTCCAACCAGGCTCCTGTGATAGAGTTCTTTTAAGCCCAAGATTTTTTATTTGAGGGTTTTT
TGTTTTTTAAAAAAAAATTGAACAAAGACTACTAATGACTTTGTTTGAATTATCCACATGAAAATAAAGAGCCATAGTTTCA
- refseq_APEX1.F1 (forward primer)
- refseq_APEX1.R1 (reverse primer)
- Alternative donor
Frame 0
QTDQSRAFFGHDKRASDHVTRSATHVGAQRAPFFVLGLGGARLPSGRCRYGVALLLIRGASLAV*TA
TR*KYCFGG*RGTAAQGRS*RECRSVGKRERWR
KTGMSSGQSQRPRRVRRPQRKMTKRQQERAQPC
MRTPQIRKPHPVANLPHSRSALGMWMGFEPGLRR
KD*IG*RKKPQIYCAFKRPNVQRTNYQLNFRSC
LDSLINTGQLLRTRKGTVAWACFPASAHSKFLT
A*AMRSMIRKAG*LWLNLTRLCW*QHMYLMQAEV
WYDWSTGSAGMKPFASS*RAWLPESPLCCVETS
MWHMKKLTFATPRGTKRMLASRHKSAKASGNYC
RLCHWLTALGTSTPTHPMPTPFGLI**MLDPRML
VGALITFCCPTLCYLHCVTARSVPRPSAVITVL
SPYT*HCDTTPKSL*AWEISPLNYHSFFKHSSE
KSAFYFSCIKLGILQPGSCDRVLLSPRFFI*GFF
VF*KKIEQRLLMTLFELST*K*RAIVS
Frame 1
RQTNHAHSSATTSAPLIT*PGPLPTWGLSVHPSLCSG*EELGCHRAGADTGLLFCS*EGLRWQSER
QRGKNIASVGDAVQLPKGVRNGNAEAWEKGSGGG
RRG*AQDRARGQEE*DGRKEK*QRGSRRGPSPV
*GPPRSENLTQWQTCHTQDLLLECGWASSLD*E
ERIRLGKGRSPRYTVPSRDQMFREQTTS*TSGAA
WTLSSILVSSFGQGRVQWRGPAFPPVPTQSFLR
HRR*GA*SGRPGDCG*I*LVCAGNSICT*CRPR
SGTTGVPAALG*SLSQVPEGPGFPKAPCAVWRPQ
CGT*RN*PSQPQGEQKECWLHATRAPRLRGITA
GCATG*QL*APLPQHTLCLHLLDLYDECSIQEC
WLAP*LLFVVPLSVTCIV*QQDPFQGPRQ*SLSY
HPIPSTVTPPLNHFEPGK*APSTTIPSLNTLQR
NLHSISHV*N*ESSNQAPVIEFF*AQDFLFEGF
LFFKKKLNKDY**LCLNYPHENKEP*F
Frame 2
DRPITRILRPRQARL*SRDQVRYPRGGSACTLLCARVRRS*AAIGPVQIRGCSFAHKRGFAGSLNG
NAVKILLRWVTRYSCPRAFVTGMPKRGKKGAVA
EDGDELRTEPEAKKSKTAAKKNDKEAAGEGPALY
EDPPDQKTSPSGKPATLKICSWNVDGLRAWIKK
KGLDWVKEEAPDILCLQETKCSENKLPAELQEL
PGLSHQYWSAPSDKEGYSGVGLLSRQCPLKVSYG
IGDEEHDQEGRVIVAEFDSFVLVTAYVPNAGRG
LVRLEYRQRWDEAFRKFLKGLASRKPLVLCGDL
NVAHEEIDLRNPKGNKKNAGFTPQERQGFGELLQ
AVPLADSFRHLYPNTPYAYTFWTYMMNARSKNV
GWRLDYFLLSHSLLPALCDSKIRSKALGSDHCP
ITLYLAL*HHP*ITLSLGNKPPQLPFLL*TLFRE
ICILFLMYKTRNPPTRLL**SSFKPKIFYLRVF
CFLKKN*TKTTNDFV*IIHMKIKSHSF
Sequence NM_001641
CAGACAGACCAATCACGCGCATTCTTCGGCCACGACAAGCGCGCCTCTGATCACGTGACCAGGTCCGCTACCCACGTGGGGGCTCAGCGTGCACCCTTCTTTGTGCTCGGGTTAGGAGGAGCTAGGCTGCCATCGGGCCGGTGCAGATACGGGGTTGCTCTTTTGCTCATAAGAGGGGCTTCGCTGGCAGTCTGAACGGC
AAGCTTGAGTCAGGACCCTTAATTAAGATCCTCAATTGGCTGGAGGGCAGATCTCGCGAGTAGGGCAACGCGGTAAAAATATTGCTTCGGTGGGTGACGC
GGTACAGCTGCCCAAGGGCGTTCGTAACGGGAATGCCGAAGCGTGGGAAAAAGGGAGCGGTGGCGGAAGACGGGGATGAGCTCAGGACAGAGCCAGAGGC
CAAGAAGAGTAAGACGGCCGCAAAGAAAAATGACAAAGAGGCAGCAGGAGAGGGCCCAGCCCTGTATGAGGACCCCCCAGATCAGAAAACCTCACCCAGT
GGCAAACCTGCCACACTCAAGATCTGCTCTTGGAATGTGGATGGGCTTCGAGCCTGGATTAAGAAGAAAGGATTAGATTGGGTAAAGGAAGAAGCCCCAG
ATATACTGTGCCTTCAAGAGACCAAATGTTCAGAGAACAAACTACCAGCTGAACTTCAGGAGCTGCCTGGACTCTCTCATCAATACTGGTCAGCTCCTTC
GGACAAGGAAGGGTACAGTGGCGTGGGCCTGCTTTCCCGCCAGTGCCCACTCAAAGTTTCTTACGGCATAGGCGATGAGGAGCATGATCAGGAAGGCCGG
GTGATTGTGGCTGAATTTGACTCGTTTGTGCTGGTAACAGCATATGTACCTAATGCAGGCCGAGGTCTGGTACGACTGGAGTACCGGCAGCGCTGGGATG
AAGCCTTTCGCAAGTTCCTGAAGGGCCTGGCTTCCCGAAAGCCCCTTGTGCTGTGTGGAGACCTCAATGTGGCACATGAAGAAATTGACCTTCGCAACCC
CAAGGGGAACAAAAAGAATGCTGGCTTCACGCCACAAGAGCGCCAAGGCTTCGGGGAATTACTGCAGGCTGTGCCACTGGCTGACAGCTTTAGGCACCTC
TACCCCAACACACCCTATGCCTACACCTTTTGGACTTATATGATGAATGCTCGATCCAAGAATGTTGGTTGGCGCCTTGATTACTTTTTGTTGTCCCACT
CTCTGTTACCTGCATTGTGTGACAGCAAGATCCGTTCCAAGGCCCTCGGCAGTGATCACTGTCCTATCACCCTATACCTAGCACTGTGACACCACCCCTA
AATCACTTTGAGCCTGGGAAATAAGCCCCCTCAACTACCATTCCTTCTTTAAACACTCTTCAGAGAAATCTGCATTCTATTTCTCATGTATAAAACTAGG
AATCCTCCAACCAGGCTCCTGTGATAGAGTTCTTTTAAGCCCAAGATTTTTTATTTGAGGGTTTTTTGTTTTTTAAAAAAAAATTGAACAAAGACTACTA
ATGACTTTGTTTGAATTATCCACATGAAAATAAAGAGCCATAGTTTCA
- refseq_APEX1.F1 (forward primer)
- refseq_APEX1.R1 (reverse primer)
- Alternative donor
Frame 0
QTDQSRAFFGHDKRASDHVTRSATHVGAQRAPFFVLGLGGARLPSGRCRYGVALLLIRGASLAV*TA
SLSQDP*LRSSIGWRADLASRATR*KYCFGG*R
GTAAQGRS*RECRSVGKRERWRKTGMSSGQSQR
PRRVRRPQRKMTKRQQERAQPCMRTPQIRKPHPV
ANLPHSRSALGMWMGFEPGLRRKD*IG*RKKPQ
IYCAFKRPNVQRTNYQLNFRSCLDSLINTGQLL
RTRKGTVAWACFPASAHSKFLTA*AMRSMIRKAG
*LWLNLTRLCW*QHMYLMQAEVWYDWSTGSAGM
KPFASS*RAWLPESPLCCVETSMWHMKKLTFAT
PRGTKRMLASRHKSAKASGNYCRLCHWLTALGTS
TPTHPMPTPFGLI**MLDPRMLVGALITFCCPT
LCYLHCVTARSVPRPSAVITVLSPYT*HCDTTP
KSL*AWEISPLNYHSFFKHSSEKSAFYFSCIKLG
ILQPGSCDRVLLSPRFFI*GFFVF*KKIEQRLL
MTLFELST*K*RAIVS
Frame 1
RQTNHAHSSATTSAPLIT*PGPLPTWGLSVHPSLCSG*EELGCHRAGADTGLLFCS*EGLRWQSER
QA*VRTLN*DPQLAGGQISRVGQRGKNIASVGDA
VQLPKGVRNGNAEAWEKGSGGGRRG*AQDRARG
QEE*DGRKEK*QRGSRRGPSPV*GPPRSENLTQ
WQTCHTQDLLLECGWASSLD*EERIRLGKGRSPR
YTVPSRDQMFREQTTS*TSGAAWTLSSILVSSF
GQGRVQWRGPAFPPVPTQSFLRHRR*GA*SGRP
GDCG*I*LVCAGNSICT*CRPRSGTTGVPAALG*
SLSQVPEGPGFPKAPCAVWRPQCGT*RN*PSQP
QGEQKECWLHATRAPRLRGITAGCATG*QL*AP
LPQHTLCLHLLDLYDECSIQECWLAP*LLFVVPL
SVTCIV*QQDPFQGPRQ*SLSYHPIPSTVTPPL
NHFEPGK*APSTTIPSLNTLQRNLHSISHV*N*
ESSNQAPVIEFF*AQDFLFEGFLFFKKKLNKDY*
*LCLNYPHENKEP*F
Frame 2
DRPITRILRPRQARL*SRDQVRYPRGGSACTLLCARVRRS*AAIGPVQIRGCSFAHKRGFAGSLNG
KLESGPLIKILNWLEGRSRE*GNAVKILLRWVT
RYSCPRAFVTGMPKRGKKGAVAEDGDELRTEPEA
KKSKTAAKKNDKEAAGEGPALYEDPPDQKTSPS
GKPATLKICSWNVDGLRAWIKKKGLDWVKEEAP
DILCLQETKCSENKLPAELQELPGLSHQYWSAPS
DKEGYSGVGLLSRQCPLKVSYGIGDEEHDQEGR
VIVAEFDSFVLVTAYVPNAGRGLVRLEYRQRWD
EAFRKFLKGLASRKPLVLCGDLNVAHEEIDLRNP
KGNKKNAGFTPQERQGFGELLQAVPLADSFRHL
YPNTPYAYTFWTYMMNARSKNVGWRLDYFLLSH
SLLPALCDSKIRSKALGSDHCPITLYLAL*HHP*
ITLSLGNKPPQLPFLL*TLFREICILFLMYKTR
NPPTRLL**SSFKPKIFYLRVFCFLKKN*TKTT
NDFV*IIHMKIKSHSF
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