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Sequences

Sequence NM_004458

GCGATTCGGCTGGCTCTGCCACACCACCGCGCGCCCCCGCTCCGCCCGCCCCTCCGGGCGCGTCTTTTCCGGGCTCGCGCTGAGTCCCGCCTCCGCCGGC TGTCCGGGTGCGCGCGCGCCGCTGCGGCTTTTTCTCTGGCCTCCGCCGCGCGCTCCTCCTCGTCCCAGCGCTAGCGGGCACGCGGTTCCTTTTTGCGAGC TTTCCGAGTGCCAGGCGCCGGCCGGCTGCGAAGACGCGGTGGGCCGCCCCTCCGATTGAAATCACAGAAGATATTCGTGTTCTTCTTAAGAGAAAAAGAG GACATTTAAAAACGCTATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACTTCGACTCACTAGCT GTAATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATCCAAGTTTGGGAAGAAGGACAGCCTTGGGACCAGGGAAATCCTAA GTGAAGAAAATGAAATGCAGCCAAATGGAAAAGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAATCGCAGAGTGAA TAACTTTGGTAGTGGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGAGACCAGGGCCGAATGGATGATTGCAGCACAGACC TGCTTTAAGTACAACTTTCCTCTTGTGACTTTATATGCCACACTTGGCAAAGAAGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTTCCTATCTGATTA CCAGTGTTGAACTTCTGGAAAGTAAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGTGGACAATAAGGCTATCAATAAAGC AGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAAGAGTTGGGATCTAACCCAGAAAACTTGGGCATTCCTCCAAGTAGACCAACGCCT TCAGACATGGCCATTGTTATGTATACTAGTGGTTCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGGAATGACAGGCCAGT GTGAAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTGGCTCATGTGCTAGAACTGACAGCAGAGATATCTTGCTTTACCTA TGGCTGCAGGATTGGATATTCTTCTCCGCTTACACTCTCTGACCAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTACTGAAGCCCACA CTTATGGCTGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAGATGAATTATATTCAGAAAACTCTGTTCAAGATAG GGTATGATTACAAATTGGAACAGATCAAAAAGGGATATGATGCACCTCTTTGCAATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCTGGGAGGGAATGT CCGCATGATGCTGTCTGGAGGGGCCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCAATTGGCCAGGGTTATGGACTGACA GAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTACTGGCAGAGTTGGAGCACCTCTTATTTGCTGTGAAATTAAGCTAAAAGACTGGC AAGAAGGCGGTTATACAATTAATGACAAGCCAAACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTTAAAAATGAAGAGAA AACAGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTGATATTGGAGAATTCCATCCCGATGGATGTTTACAGATTATAGAT CGTAAGAAAGATCTAGTGAAGTTACAAGCAGGAGAGTATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTTATTGACAACATCTGTG CTTTTGCCAAAAGTGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACACTTTTGGCACAACAGAAAGGGGTAGAAGGAACTTG GGTTGATATCTGCAATAATCCTGCTATGGAAGCTGAAATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCAATC AAGGTTCGATTAAGCCCAGAGCCATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGGAGCTGAGGAACCATTACCTCAAAG ACATTGAACGAATGTATGGGGGCAAATAAAATGTTGTTGTCTTATTGACAGTTGTGCAGGAGGTAGCCTGGTGGTTTTCAACCTCTAGAATTTTAAGCCT TTGTTGAACTGTTAGAATGTAAGGTATATCATTCTAAAGATAGAGTAAAAAGAAAACAAAACCAAAAGTTATTAAAATTGTTGTCCGGTTTACTTTAACT TAGTTTTGCATAGTTCTAGTGCAGCTGAAATTGAAAAGTTATTTCCCTTTAGCTGTGTTATTATAGAGCAGAAATTCTGTTTTTAAAAATTAGCCTAAGA TATACTTGTTTTTGTAAAGAAAAATATTTAATGTTGAACAAAATAAATTGGAGTTGGAGTAGAATGTAGTTTGAGGAAATTTGCAGCTTCCAATGCCTCT TGTCTTCCTATTTCAGAAGTTTAAATATTAAGCATGACAGAAAATATGTATTAACACTACTCAAAGCAAAAGTGCTGCAGGGCTTTAAAATTCTCTTCCA ACCATTTATCTTGAAGGAAAAATTCAATAGTAATATAATACACAAAATCAAATAATACCTTAGAAGGTATTAAGATTATAATTGTTGCATAGGTTAGATA TAGAGTCATTGTAATGTTGTGAATAATTACAGTGCCTAAAATAAGAATAGAACAACATATACAACACCAAAAAATATCTAGTAATATATTTAAAGGGAAA TTGAGCTGCTTTTTTTGAAACTTTGAGATCTAAAAATAACTGTAATTATTTGAATGACTAAGAGGAAAGTACATTTTTTGAAATGCTGAAAATTGCCTTT CTGTGTTTATTCAAACTGAAAAGCTGAGACCAAGAGCAAGGAAGGTAAAAAGTTAACAGGCAAACATTTTCTCTTAGAAAAGGTGATAAAATCATAAGTA TTTGGAATTAGAACCCTTGCACAGCACTGAACCTGGGAAAGAGATTTAAACTCTGAATTTATCTTTGATAACAGGGATTGATTTTAAAATGTACATGTAT TAAATTACATTTGTAATTTAAGGTCTGTTTGCTGTTGCTGATTTTATTCTTGATCAGTAGTTTGCATTTCAGAAAGCCTTTCATTTTGCTTTAATTTTAG CAAAGCGGGTTATAATGAATGACTTCCCCAATATCTTGCTTGAACTTACAGGTGATTAACTTGGATGAGTTTTGGGAAGTTAAAGGGAAGAAAACACTGT TATCATTTTTTCCTGTTTGGGAAGAGCTTAGAAACTGGAAATACTAGATTTGGGAGAAGGGCAGAGTTACTTGATAAGGGACTGATGTTTGTGCAGTAAC TTGGGAGTGTGGTTTCTTTTTGAATCTTTAATTAAAACCTGGGATTATATATCCCTGATAAATATTCACACTTGAACCATAGTTACTGTAAAATGCAAAA AATCTTAATACTGTTATTCTTTGCACTTTTTCTTAATCATTTTTTATATATATGCATATATATATGTGTGTGTGTGTGTTGCTTATGTTGTTTTGTACAG ATGTGGGCCACCATTGCAACAAAATACATTCTTTTTGCTCTAAAATATTTATGAAGAAAATACTTAAATGTTATGTATATGGTGGTAATAAGGGAAAAAT CAAGTATTATAAACAAGAATGAAGGTTTTTGTAAAGATTTCTGTTCAGCGTTTTGCAAGGTAAAATTTTAGGCAAGTTTTCCCTGAAGTTATGTGTATGT GAGTATTCTCATTCTTCCCAACTTGCCTTTGAAGAGTGAAATACCATTATTATCAAGTAGACTACTGTTCAGCTTTTATTCCTGCCCTGCTGTTTATCCC TTAAGAATGAGTTTCTTAGACTTTTCCAATATGTGATTTTTTTTCCCATTTAGAATGGTGATTTTAAATGTGTGAGTGCATGTACTATCTTATCTCAGAT ATTTGCACCCCCAATCTGCCCCCAACTCCCAAAAGCTAGAACACTGCCAACTGATCTGTTATAGGTCCTTTAGAAACACATAATTAACACTTAAGGTTGG GTGCTGCTAATTCTTTGCAAAAATCCAAATATTGTTAAGGGACCAGGGAGATGCCACTACCCCTTGATTTTCCATCTAAAAATATACATGTTTATGTAAA CAAATCTTTCCATATCCATAGTGACTTTTCAAGTATTTAAGCCTAAAGATTTTGATCTCACATTTTTATACCTGTTTAAATTGCTCACAGTTATTACATA CACATCAGCCATCAACTAAAGTTGTACTTTAAAAATTTACTACAATATGTACATTTCTAAGTCAAACACTTGTGACTTTTGCTTTAATTCCATGAATGTT CCTGCCTCCTTGATATTTGTATTTATTCTTTTTTTCTCTAGAGTAGAGGTATAATTGTGTGATATTTCAGAAATACAGATAAATGATTCAAAAAGTCACA GTTAAGGAGAATCATGTTTCTTTGATCATGAATAACTGATTAGTAAGTCTTGCCTATATTTTCCTGATAGCATATGACAAATGTTTCTAAGGTAACAAGA TGAGAACAGATAAAGATTGTGTGGTGTTTTGGATTTGGAGAGAAATATTTTAATTTTTAAATGCAGTTACAAATTATAATGTATTCATATTTGTACTTTC TGTTAAAATGCATGATTGCAGAATTGTTTAGATTTTGTGTTTATTCTTGATGAAAAGCTTTGTTTGTTCTTGTTTTTAAGTTTGCACTCAAATCTTAAGA AATAAATCCACCCATGTTATCAA

refseq_ACSL4.F1 (forward primer)
refseq_ACSL4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AIRLALPHHRAPPLRPPLRARLFRARAESRLRR LSGCARAAAAFSLASAARSSSSQR*RARGSFLRA FRVPGAGRLRRRGGPPLRLKSQKIFVFFLREKE DI*KRYGKENKS*AHFRQTWKSISLCHTLRLTS CNRHPWSRYSG*II*PCCIQVWEEGQPWDQGNPK *RK*NAAKWKSF*EVNSWEL*MDELS*SESQSE *LW*WTHCTGTKTKEHHCHLL*DQGRMDDCSTD LL*VQLSSCDFICHTWQRSSSSWAK*I*GFLSDY QC*TSGK*T*DCIVRYQLC*TYHLCGQ*GYQ*S RVP*RI*DSQHAISRRVGI*PRKLGHSSK*TNA FRHGHCYVY*WFYWPT*GSDDAS*QFDSWNDRPV *KNTWTGTEGHIYWLLAFGSCARTDSRDILLYL WLQDWIFFSAYTL*PVQQN*KRKQRRLYCTEAH TYGCCSGNHG*NL*ECYEQSPRDELYSENSVQDR V*LQIGTDQKGI*CTSLQSVTV*KGQGPAGREC PHDAVWRGPAISSDTPIHECLLLLPNWPGLWTD RIMWCWDSY*SN*LYYWQSWSTSYLL*N*AKRLA RRRLYN**QAKPQR*NRNWWTEHLHGIF*K*RE NSRRLFCG*KWTKVVLHW*YWRIPSRWMFTDYR S*ERSSEVTSRRVCISWESRSCTEELSTY*QHLC FCQK*SVLCDQFCGS*PEKVDTFGTTERGRRNL G*YLQ*SCYGS*NTERNSRSCKCHEIGAI*NSN QGSIKPRAMDP*NWFGN*CFQTEKEGAEEPLPQR H*TNVWGQIKCCCLIDSCAGGSLVVFNL*NFKP LLNC*NVRYIILKIE*KENKTKSY*NCCPVYFN LVLHSSSAAEIEKLFPFSCVIIEQKFCF*KLA*D ILVFVKKNI*C*TK*IGVGVECSLRKFAASNAS CLPISEV*ILSMTENMY*HYSKQKCCRALKFSS NHLS*RKNSIVI*YTKSNNTLEGIKIIIVA*VRY RVIVML*IITVPKIRIEQHIQHQKISSNIFKGK LSCFF*NFEI*K*L*LFE*LRGKYIF*NAENCL SVFIQTEKLRPRARKVKS*QANIFS*KR**NHKY LELEPLHSTEPGKEI*TLNLSLITGIDFKMYMY *ITFVI*GLFAVADFILDQ*FAFQKAFHFALIL AKRVIMNDFPNILLELTGD*LG*VLGS*REENTV IIFSCLGRA*KLEILDLGEGQSYLIRD*CLCSN LGVWFLFESLIKTWDYISLINIHT*TIVTVKCK KS*YCYSLHFFLIIFYIYAYIYVCVCVAYVVLYR CGPPLQQNTFFLL*NIYEENT*MLCIWW**GKN QVL*TRMKVFVKISVQRFAR*NFRQVFPEVMCM *VFSFFPTCL*RVKYHYYQVDYCSAFIPALLFIP *E*VS*TFPICDFFSHLEW*F*MCECMYYLISD ICTPNLPPTPKS*NTAN*SVIGPLETHN*HLRL GAANSLQKSKYC*GTREMPLPLDFPSKNIHVYVN KSFHIHSDFSSI*A*RF*SHIFIPV*IAHSYYI HISHQLKLYFKNLLQYVHF*VKHL*LLL*FHEC SCLLDICIYSFFL*SRGIIV*YFRNTDK*FKKSQ LRRIMFL*S*ITD**VLPIFS**HMTNVSKVTR *EQIKIVWCFGFGEKYFNF*MQLQIIMYSYLYF LLKCMIAELFRFCVYS**KALFVLVFKFALKS*E INPPMLS

Frame 1

RFGWLCHTTARPRSARPSGRVFSGLALSPASAG CPGARAPLRLFLWPPPRAPPRPSASGHAVPFCE LSECQAPAGCEDAVGRPSD*NHRRYSCSS*EKKR TFKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLA VIDIPGADTLDKLFDHAVSKFGKKDSLGTREIL SEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVN NFGSGLTALGLKPKNTIAIFCETRAEWMIAAQT CFKYNFPLVTLYATLGKEAVVHGLNESEASYLI TSVELLESKLKTALLDISCVKHIIYVDNKAINKA EYPEGFEIHSMQSVEELGSNPENLGIPPSRPTP SDMAIVMYTSGSTGRPKGVMMHHSNLIAGMTGQ CERIPGLGPKDTYIGYLPLAHVLELTAEISCFTY GCRIGYSSPLTLSDQSSKIKKGSKGDCTVLKPT LMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKI GYDYKLEQIKKGYDAPLCNLLLFKKVKALLGGNV RMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLT ESCGAGTVTEVTDYTTGRVGAPLICCEIKLKDW QEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEK TAEDYSVDENGQRWFCTGDIGEFHPDGCLQIID RKKDLVKLQAGEYVSLGKVEAALKNCPLIDNIC AFAKSDQSYVISFVVPNQKRLTLLAQQKGVEGTW VDICNNPAMEAEILKEIREAANAMKLERFEIPI KVRLSPEPWTPETGLVTDAFKLKRKELRNHYLK DIERMYGGK*NVVVLLTVVQEVAWWFSTSRILSL C*TVRM*GISF*R*SKKKTKPKVIKIVVRFTLT *FCIVLVQLKLKSYFPLAVLL*SRNSVFKN*PK IYLFL*RKIFNVEQNKLELE*NVV*GNLQLPMPL VFLFQKFKY*A*QKICINTTQSKSAAGL*NSLP TIYLEGKIQ**YNTQNQIIP*KVLRL*LLHRLD IESL*CCE*LQCLK*E*NNIYNTKKYLVIYLKGN *AAFFETLRSKNNCNYLND*EESTFFEMLKIAF LCLFKLKS*DQEQGR*KVNRQTFSLRKGDKIIS IWN*NPCTALNLGKRFKL*IYL**QGLILKCTCI KLHL*FKVCLLLLILFLISSLHFRKPFILL*F* QSGL**MTSPISCLNLQVINLDEFWEVKGKKTL LSFFPVWEELRNWKY*IWEKGRVT**GTDVCAVT WECGFFLNL*LKPGIIYP**IFTLEP*LL*NAK NLNTVILCTFS*SFFIYMHIYMCVCVLLMLFCT DVGHHCNKIHSFCSKIFMKKILKCYVYGGNKGKI KYYKQE*RFL*RFLFSVLQGKILGKFSLKLCVC EYSHSSQLAFEE*NTIIIK*TTVQLLFLPCCLS LKNEFLRLFQYVIFFPI*NGDFKCVSACTILSQI FAPPICPQLPKARTLPTDLL*VL*KHIINT*GW VLLILCKNPNIVKGPGRCHYPLIFHLKIYMFM* TNLSISIVTFQVFKPKDFDLTFLYLFKLLTVITY TSAIN*SCTLKIYYNMYISKSNTCDFCFNSMNV PASLIFVFILFFSRVEV*LCDISEIQINDSKSH S*GESCFFDHE*LISKSCLYFPDSI*QMFLR*QD ENR*RLCGVLDLERNILIFKCSYKL*CIHICTF C*NA*LQNCLDFVFILDEKLCLFLFLSLHSNLK K*IHPCYQ

Frame 2

DSAGSATPPRAPAPPAPPGASFPGSR*VPPPPA VRVRARRCGFFSGLRRALLLVPALAGTRFLFAS FPSARRRPAAKTRWAAPPIEITEDIRVLLKRKR GHLKTLWQRE*KLSPLQTNLEVHIALSHTSTH*L **TSLEQILWINYLTMLYPSLGRRTALGPGKS* VKKMKCSQMEKFLRS*FLGIING*TILK*IAE* ITLVVDSLHWD*NQRTPLPSSVRPGPNG*LQHRP ALSTTFLL*LYMPHLAKKQ*FMG*MNLRLPI*L PVLNFWKVNLRLHC*ISVVLNISFMWTIRLSIK QSTLKDLRFTACNQ*KSWDLTQKTWAFLQVDQRL QTWPLLCILVVLLADLRE**CIIAI**LE*QAS VKEYLDWDRRTHILATCLWLMC*N*QQRYLALP MAAGLDILLRLHSLTSPAKLKKEAKETVLY*SPH LWLLFRKSWIEFIRML*AKSKR*IIFRKLCSR* GMITNWNRSKRDMMHLFAICYCLKRSRPCWEGM SA*CCLEGPRYLLRHTDS*MSASAAQLARVMD*Q NHVVLGQLLK*LTILLAELEHLLFAVKLS*KTG KKAVIQLMTSQTPEVKS*LVDRTSPWDILKMKR KQQKIILWMKMDKGGFALVILENSIPMDVYRL*I VRKI**SYKQESMYLLGK*KLH*RIVHLLTTSV LLPKVISPM*SVLWFLTRKG*HFWHNRKG*KEL GLISAIILLWKLKY*KKFEKLQMP*NWSDLKFQS RFD*AQSHGPLKLVW*LMLSN*KGRS*GTITSK TLNECMGANKMLLSY*QLCRR*PGGFQPLEF*A FVELLECKVYHSKDRVKRKQNQKLLKLLSGLL*L SFA*F*CS*N*KVISL*LCYYRAEILFLKISLR YTCFCKEKYLMLNKINWSWSRM*FEEICSFQCL LSSYFRSLNIKHDRKYVLTLLKAKVLQGFKILFQ PFILKEKFNSNIIHKIK*YLRRY*DYNCCIG*I *SHCNVVNNYSA*NKNRTTYTTPKNI**YI*RE IELLFLKL*DLKITVII*MTKRKVHFLKC*KLPF CVYSN*KAETKSKEGKKLTGKHFLLEKVIKS*V FGIRTLAQH*TWERDLNSEFIFDNRD*F*NVHV LNYICNLRSVCCC*FYS*SVVCISESLSFCFNFS KAGYNE*LPQYLA*TYR*LTWMSFGKLKGRKHC YHFFLFGKSLETGNTRFGRRAELLDKGLMFVQ* LGSVVSF*IFN*NLGLYIPDKYSHLNHSYCKMQK ILILLFFALFLNHFLYICIYICVCVCCLCCFVQ MWATIATKYILFALKYL*RKYLNVMYMVVIREK SSIINKNEGFCKDFCSAFCKVKF*ASFP*SYVYV SILILPNLPLKSEIPLLSSRLLFSFYSCPAVYP LRMSFLDFSNM*FFFPFRMVILNV*VHVLSYLR YLHPQSAPNSQKLEHCQLICYRSFRNT*LTLKVG CC*FFAKIQILLRDQGDATTP*FSI*KYTCLCK QIFPYP**LFKYLSLKILISHFYTCLNCSQLLH THQPSTKVVL*KFTTICTFLSQTLVTFALIP*MF LPP*YLYLFFFSLE*RYNCVIFQKYR*MIQKVT VKENHVSLIMNN*LVSLAYIFLIAYDKCF*GNK MRTDKDCVVFWIWREIF*FLNAVTNYNVFIFVLS VKMHDCRIV*ILCLFLMKSFVCSCF*VCTQILR NKSTHVI

Sequence NM_022977

GCGATTCGGCTGGCTCTGCCACACCACCGCGCGCCCCCGCTCCGCCCGCCCCTCCGGGCGCGTCTTTTCCGGGCTCGCGCTGAGTCCCGCCTCCGCCGGC TGTCCGGGTGCGCGCGCGCCGCTGCGGCTTTTTCTCTGGCCTCCGCCGCGCGCTCCTCCTCGTCCCAGCGCTAGCGGGCACGCGGTTCCTTTTTGCGAGC TTTCCGAGTGCCAGGCGCCGGCCGGCTGCGAAGACGCGGTGGGCCGCCCCTCCGATTGAAATCACAGAAGATATTCGTGTTCTTCTTAAGAGAAAAAGAG GACATTTTAGCTTTCTCAGTTGAAGGCGTACTTTATTGTCGGCTTCCAAAGATTACTAACTTTTATCTGTATCACTAAGATTGAACTGCCTTGGCTGTAC TGCTATTCTTACTGCTGCTTCTATTATTGCCTTCTTCAGCACAATAAGGCTTTCAAAAGCCAAAGAATAACAAGAAATAAGCACCATTTTAGAAGCCTTT CCACTATGAAACTTAAGCTAAATGTGCTCACCATTATTTTGCTGCCTGTCCACTTGTTAATAACAATATACAGTGCCCTTATATTTATTCCATGGTATTT TCTTACCAATGCCAAGAAGAAAAACGCTATGGCAAAGAGAATAAAAGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACTTC GACTCACTAGCTGTAATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATCCAAGTTTGGGAAGAAGGACAGCCTTGGGACCA GGGAAATCCTAAGTGAAGAAAATGAAATGCAGCCAAATGGAAAAGTTTTTAAGAAGTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAA TCGCAGAGTGAATAACTTTGGTAGTGGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGAGACCAGGGCCGAATGGATGATT GCAGCACAGACCTGCTTTAAGTACAACTTTCCTCTTGTGACTTTATATGCCACACTTGGCAAAGAAGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTT CCTATCTGATTACCAGTGTTGAACTTCTGGAAAGTAAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGTGGACAATAAGGC TATCAATAAAGCAGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAAGAGTTGGGATCTAACCCAGAAAACTTGGGCATTCCTCCAAGT AGACCAACGCCTTCAGACATGGCCATTGTTATGTATACTAGTGGTTCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGGAA TGACAGGCCAGTGTGAAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTGGCTCATGTGCTAGAACTGACAGCAGAGATATC TTGCTTTACCTATGGCTGCAGGATTGGATATTCTTCTCCGCTTACACTCTCTGACCAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTA CTGAAGCCCACACTTATGGCTGCTGTTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAGATGAATTATATTCAGAAAACTC TGTTCAAGATAGGGTATGATTACAAATTGGAACAGATCAAAAAGGGATATGATGCACCTCTTTGCAATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCT GGGAGGGAATGTCCGCATGATGCTGTCTGGAGGGGCCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCAATTGGCCAGGGT TATGGACTGACAGAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTACTGGCAGAGTTGGAGCACCTCTTATTTGCTGTGAAATTAAGC TAAAAGACTGGCAAGAAGGCGGTTATACAATTAATGACAAGCCAAACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTTAA AAATGAAGAGAAAACAGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTGATATTGGAGAATTCCATCCCGATGGATGTTTA CAGATTATAGATCGTAAGAAAGATCTAGTGAAGTTACAAGCAGGAGAGTATGTATCTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTTATTG ACAACATCTGTGCTTTTGCCAAAAGTGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACACTTTTGGCACAACAGAAAGGGGT AGAAGGAACTTGGGTTGATATCTGCAATAATCCTGCTATGGAAGCTGAAATACTGAAAGAAATTCGAGAAGCTGCAAATGCCATGAAATTGGAGCGATTT GAAATTCCAATCAAGGTTCGATTAAGCCCAGAGCCATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGGAGCTGAGGAACC ATTACCTCAAAGACATTGAACGAATGTATGGGGGCAAATAAAATGTTGTTGTCTTATTGACAGTTGTGCAGGAGGTAGCCTGGTGGTTTTCAACCTCTAG AATTTTAAGCCTTTGTTGAACTGTTAGAATGTAAGGTATATCATTCTAAAGATAGAGTAAAAAGAAAACAAAACCAAAAGTTATTAAAATTGTTGTCCGG TTTACTTTAACTTAGTTTTGCATAGTTCTAGTGCAGCTGAAATTGAAAAGTTATTTCCCTTTAGCTGTGTTATTATAGAGCAGAAATTCTGTTTTTAAAA ATTAGCCTAAGATATACTTGTTTTTGTAAAGAAAAATATTTAATGTTGAACAAAATAAATTGGAGTTGGAGTAGAATGTAGTTTGAGGAAATTTGCAGCT TCCAATGCCTCTTGTCTTCCTATTTCAGAAGTTTAAATATTAAGCATGACAGAAAATATGTATTAACACTACTCAAAGCAAAAGTGCTGCAGGGCTTTAA AATTCTCTTCCAACCATTTATCTTGAAGGAAAAATTCAATAGTAATATAATACACAAAATCAAATAATACCTTAGAAGGTATTAAGATTATAATTGTTGC ATAGGTTAGATATAGAGTCATTGTAATGTTGTGAATAATTACAGTGCCTAAAATAAGAATAGAACAACATATACAACACCAAAAAATATCTAGTAATATA TTTAAAGGGAAATTGAGCTGCTTTTTTTGAAACTTTGAGATCTAAAAATAACTGTAATTATTTGAATGACTAAGAGGAAAGTACATTTTTTGAAATGCTG AAAATTGCCTTTCTGTGTTTATTCAAACTGAAAAGCTGAGACCAAGAGCAAGGAAGGTAAAAAGTTAACAGGCAAACATTTTCTCTTAGAAAAGGTGATA AAATCATAAGTATTTGGAATTAGAACCCTTGCACAGCACTGAACCTGGGAAAGAGATTTAAACTCTGAATTTATCTTTGATAACAGGGATTGATTTTAAA ATGTACATGTATTAAATTACATTTGTAATTTAAGGTCTGTTTGCTGTTGCTGATTTTATTCTTGATCAGTAGTTTGCATTTCAGAAAGCCTTTCATTTTG CTTTAATTTTAGCAAAGCGGGTTATAATGAATGACTTCCCCAATATCTTGCTTGAACTTACAGGTGATTAACTTGGATGAGTTTTGGGAAGTTAAAGGGA AGAAAACACTGTTATCATTTTTTCCTGTTTGGGAAGAGCTTAGAAACTGGAAATACTAGATTTGGGAGAAGGGCAGAGTTACTTGATAAGGGACTGATGT TTGTGCAGTAACTTGGGAGTGTGGTTTCTTTTTGAATCTTTAATTAAAACCTGGGATTATATATCCCTGATAAATATTCACACTTGAACCATAGTTACTG TAAAATGCAAAAAATCTTAATACTGTTATTCTTTGCACTTTTTCTTAATCATTTTTTATATATATGCATATATATATGTGTGTGTGTGTGTTGCTTATGT TGTTTTGTACAGATGTGGGCCACCATTGCAACAAAATACATTCTTTTTGCTCTAAAATATTTATGAAGAAAATACTTAAATGTTATGTATATGGTGGTAA TAAGGGAAAAATCAAGTATTATAAACAAGAATGAAGGTTTTTGTAAAGATTTCTGTTCAGCGTTTTGCAAGGTAAAATTTTAGGCAAGTTTTCCCTGAAG TTATGTGTATGTGAGTATTCTCATTCTTCCCAACTTGCCTTTGAAGAGTGAAATACCATTATTATCAAGTAGACTACTGTTCAGCTTTTATTCCTGCCCT GCTGTTTATCCCTTAAGAATGAGTTTCTTAGACTTTTCCAATATGTGATTTTTTTTCCCATTTAGAATGGTGATTTTAAATGTGTGAGTGCATGTACTAT CTTATCTCAGATATTTGCACCCCCAATCTGCCCCCAACTCCCAAAAGCTAGAACACTGCCAACTGATCTGTTATAGGTCCTTTAGAAACACATAATTAAC ACTTAAGGTTGGGTGCTGCTAATTCTTTGCAAAAATCCAAATATTGTTAAGGGACCAGGGAGATGCCACTACCCCTTGATTTTCCATCTAAAAATATACA TGTTTATGTAAACAAATCTTTCCATATCCATAGTGACTTTTCAAGTATTTAAGCCTAAAGATTTTGATCTCACATTTTTATACCTGTTTAAATTGCTCAC AGTTATTACATACACATCAGCCATCAACTAAAGTTGTACTTTAAAAATTTACTACAATATGTACATTTCTAAGTCAAACACTTGTGACTTTTGCTTTAAT TCCATGAATGTTCCTGCCTCCTTGATATTTGTATTTATTCTTTTTTTCTCTAGAGTAGAGGTATAATTGTGTGATATTTCAGAAATACAGATAAATGATT CAAAAAGTCACAGTTAAGGAGAATCATGTTTCTTTGATCATGAATAACTGATTAGTAAGTCTTGCCTATATTTTCCTGATAGCATATGACAAATGTTTCT AAGGTAACAAGATGAGAACAGATAAAGATTGTGTGGTGTTTTGGATTTGGAGAGAAATATTTTAATTTTTAAATGCAGTTACAAATTATAATGTATTCAT ATTTGTACTTTCTGTTAAAATGCATGATTGCAGAATTGTTTAGATTTTGTGTTTATTCTTGATGAAAAGCTTTGTTTGTTCTTGTTTTTAAGTTTGCACT CAAATCTTAAGAAATAAATCCACCCATGTTATCAA

refseq_ACSL4.F1 (forward primer)
refseq_ACSL4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

AIRLALPHHRAPPLRPPLRARLFRARAESRLRR LSGCARAAAAFSLASAARSSSSQR*RARGSFLRA FRVPGAGRLRRRGGPPLRLKSQKIFVFFLREKE DILAFSVEGVLYCRLPKITNFYLYH*D*TALAV LLFLLLLLLLPSSAQ*GFQKPKNNKK*APF*KPF HYET*AKCAHHYFAACPLVNNNIQCPYIYSMVF SYQCQEEKRYGKENKS*AHFRQTWKSISLCHTL RLTSCNRHPWSRYSG*II*PCCIQVWEEGQPWDQ GNPK*RK*NAAKWKSF*EVNSWEL*MDELS*SE SQSE*LW*WTHCTGTKTKEHHCHLL*DQGRMDD CSTDLL*VQLSSCDFICHTWQRSSSSWAK*I*GF LSDYQC*TSGK*T*DCIVRYQLC*TYHLCGQ*G YQ*SRVP*RI*DSQHAISRRVGI*PRKLGHSSK *TNAFRHGHCYVY*WFYWPT*GSDDAS*QFDSWN DRPV*KNTWTGTEGHIYWLLAFGSCARTDSRDI LLYLWLQDWIFFSAYTL*PVQQN*KRKQRRLYC TEAHTYGCCSGNHG*NL*ECYEQSPRDELYSENS VQDRV*LQIGTDQKGI*CTSLQSVTV*KGQGPA GRECPHDAVWRGPAISSDTPIHECLLLLPNWPG LWTDRIMWCWDSY*SN*LYYWQSWSTSYLL*N*A KRLARRRLYN**QAKPQR*NRNWWTEHLHGIF* K*RENSRRLFCG*KWTKVVLHW*YWRIPSRWMF TDYRS*ERSSEVTSRRVCISWESRSCTEELSTY* QHLCFCQK*SVLCDQFCGS*PEKVDTFGTTERG RRNLG*YLQ*SCYGS*NTERNSRSCKCHEIGAI *NSNQGSIKPRAMDP*NWFGN*CFQTEKEGAEEP LPQRH*TNVWGQIKCCCLIDSCAGGSLVVFNL* NFKPLLNC*NVRYIILKIE*KENKTKSY*NCCP VYFNLVLHSSSAAEIEKLFPFSCVIIEQKFCF*K LA*DILVFVKKNI*C*TK*IGVGVECSLRKFAA SNASCLPISEV*ILSMTENMY*HYSKQKCCRAL KFSSNHLS*RKNSIVI*YTKSNNTLEGIKIIIVA *VRYRVIVML*IITVPKIRIEQHIQHQKISSNI FKGKLSCFF*NFEI*K*L*LFE*LRGKYIF*NA ENCLSVFIQTEKLRPRARKVKS*QANIFS*KR** NHKYLELEPLHSTEPGKEI*TLNLSLITGIDFK MYMY*ITFVI*GLFAVADFILDQ*FAFQKAFHF ALILAKRVIMNDFPNILLELTGD*LG*VLGS*RE ENTVIIFSCLGRA*KLEILDLGEGQSYLIRD*C LCSNLGVWFLFESLIKTWDYISLINIHT*TIVT VKCKKS*YCYSLHFFLIIFYIYAYIYVCVCVAYV VLYRCGPPLQQNTFFLL*NIYEENT*MLCIWW* *GKNQVL*TRMKVFVKISVQRFAR*NFRQVFPE VMCM*VFSFFPTCL*RVKYHYYQVDYCSAFIPAL LFIP*E*VS*TFPICDFFSHLEW*F*MCECMYY LISDICTPNLPPTPKS*NTAN*SVIGPLETHN* HLRLGAANSLQKSKYC*GTREMPLPLDFPSKNIH VYVNKSFHIHSDFSSI*A*RF*SHIFIPV*IAH SYYIHISHQLKLYFKNLLQYVHF*VKHL*LLL* FHECSCLLDICIYSFFL*SRGIIV*YFRNTDK*F KKSQLRRIMFL*S*ITD**VLPIFS**HMTNVS KVTR*EQIKIVWCFGFGEKYFNF*MQLQIIMYS YLYFLLKCMIAELFRFCVYS**KALFVLVFKFAL KS*EINPPMLS

Frame 1

RFGWLCHTTARPRSARPSGRVFSGLALSPASAG CPGARAPLRLFLWPPPRAPPRPSASGHAVPFCE LSECQAPAGCEDAVGRPSD*NHRRYSCSS*EKKR TF*LSQLKAYFIVGFQRLLTFICITKIELPWLY CYSYCCFYYCLLQHNKAFKSQRITRNKHHFRSL STMKLKLNVLTIILLPVHLLITIYSALIFIPWYF LTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHF DSLAVIDIPGADTLDKLFDHAVSKFGKKDSLGT REILSEENEMQPNGKVFKKLILGNYKWMNYLEVN RRVNNFGSGLTALGLKPKNTIAIFCETRAEWMI AAQTCFKYNFPLVTLYATLGKEAVVHGLNESEA SYLITSVELLESKLKTALLDISCVKHIIYVDNKA INKAEYPEGFEIHSMQSVEELGSNPENLGIPPS RPTPSDMAIVMYTSGSTGRPKGVMMHHSNLIAG MTGQCERIPGLGPKDTYIGYLPLAHVLELTAEIS CFTYGCRIGYSSPLTLSDQSSKIKKGSKGDCTV LKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKT LFKIGYDYKLEQIKKGYDAPLCNLLLFKKVKALL GGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQG YGLTESCGAGTVTEVTDYTTGRVGAPLICCEIK LKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFK NEEKTAEDYSVDENGQRWFCTGDIGEFHPDGCL QIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLI DNICAFAKSDQSYVISFVVPNQKRLTLLAQQKGV EGTWVDICNNPAMEAEILKEIREAANAMKLERF EIPIKVRLSPEPWTPETGLVTDAFKLKRKELRN HYLKDIERMYGGK*NVVVLLTVVQEVAWWFSTSR ILSLC*TVRM*GISF*R*SKKKTKPKVIKIVVR FTLT*FCIVLVQLKLKSYFPLAVLL*SRNSVFK N*PKIYLFL*RKIFNVEQNKLELE*NVV*GNLQL PMPLVFLFQKFKY*A*QKICINTTQSKSAAGL* NSLPTIYLEGKIQ**YNTQNQIIP*KVLRL*LL HRLDIESL*CCE*LQCLK*E*NNIYNTKKYLVIY LKGN*AAFFETLRSKNNCNYLND*EESTFFEML KIAFLCLFKLKS*DQEQGR*KVNRQTFSLRKGD KIISIWN*NPCTALNLGKRFKL*IYL**QGLILK CTCIKLHL*FKVCLLLLILFLISSLHFRKPFIL L*F*QSGL**MTSPISCLNLQVINLDEFWEVKG KKTLLSFFPVWEELRNWKY*IWEKGRVT**GTDV CAVTWECGFFLNL*LKPGIIYP**IFTLEP*LL *NAKNLNTVILCTFS*SFFIYMHIYMCVCVLLM LFCTDVGHHCNKIHSFCSKIFMKKILKCYVYGGN KGKIKYYKQE*RFL*RFLFSVLQGKILGKFSLK LCVCEYSHSSQLAFEE*NTIIIK*TTVQLLFLP CCLSLKNEFLRLFQYVIFFPI*NGDFKCVSACTI LSQIFAPPICPQLPKARTLPTDLL*VL*KHIIN T*GWVLLILCKNPNIVKGPGRCHYPLIFHLKIY MFM*TNLSISIVTFQVFKPKDFDLTFLYLFKLLT VITYTSAIN*SCTLKIYYNMYISKSNTCDFCFN SMNVPASLIFVFILFFSRVEV*LCDISEIQIND SKSHS*GESCFFDHE*LISKSCLYFPDSI*QMFL R*QDENR*RLCGVLDLERNILIFKCSYKL*CIH ICTFC*NA*LQNCLDFVFILDEKLCLFLFLSLH SNLKK*IHPCYQ

Frame 2

DSAGSATPPRAPAPPAPPGASFPGSR*VPPPPA VRVRARRCGFFSGLRRALLLVPALAGTRFLFAS FPSARRRPAAKTRWAAPPIEITEDIRVLLKRKR GHFSFLS*RRTLLSASKDY*LLSVSLRLNCLGCT AILTAASIIAFFSTIRLSKAKE*QEISTILEAF PL*NLS*MCSPLFCCLSTC**QYTVPLYLFHGI FLPMPRRKTLWQRE*KLSPLQTNLEVHIALSHTS TH*L**TSLEQILWINYLTMLYPSLGRRTALGP GKS*VKKMKCSQMEKFLRS*FLGIING*TILK* IAE*ITLVVDSLHWD*NQRTPLPSSVRPGPNG*L QHRPALSTTFLL*LYMPHLAKKQ*FMG*MNLRL PI*LPVLNFWKVNLRLHC*ISVVLNISFMWTIR LSIKQSTLKDLRFTACNQ*KSWDLTQKTWAFLQV DQRLQTWPLLCILVVLLADLRE**CIIAI**LE *QASVKEYLDWDRRTHILATCLWLMC*N*QQRY LALPMAAGLDILLRLHSLTSPAKLKKEAKETVLY *SPHLWLLFRKSWIEFIRML*AKSKR*IIFRKL CSR*GMITNWNRSKRDMMHLFAICYCLKRSRPC WEGMSA*CCLEGPRYLLRHTDS*MSASAAQLARV MD*QNHVVLGQLLK*LTILLAELEHLLFAVKLS *KTGKKAVIQLMTSQTPEVKS*LVDRTSPWDIL KMKRKQQKIILWMKMDKGGFALVILENSIPMDVY RL*IVRKI**SYKQESMYLLGK*KLH*RIVHLL TTSVLLPKVISPM*SVLWFLTRKG*HFWHNRKG *KELGLISAIILLWKLKY*KKFEKLQMP*NWSDL KFQSRFD*AQSHGPLKLVW*LMLSN*KGRS*GT ITSKTLNECMGANKMLLSY*QLCRR*PGGFQPL EF*AFVELLECKVYHSKDRVKRKQNQKLLKLLSG LL*LSFA*F*CS*N*KVISL*LCYYRAEILFLK ISLRYTCFCKEKYLMLNKINWSWSRM*FEEICS FQCLLSSYFRSLNIKHDRKYVLTLLKAKVLQGFK ILFQPFILKEKFNSNIIHKIK*YLRRY*DYNCC IG*I*SHCNVVNNYSA*NKNRTTYTTPKNI**Y I*REIELLFLKL*DLKITVII*MTKRKVHFLKC* KLPFCVYSN*KAETKSKEGKKLTGKHFLLEKVI KS*VFGIRTLAQH*TWERDLNSEFIFDNRD*F* NVHVLNYICNLRSVCCC*FYS*SVVCISESLSFC FNFSKAGYNE*LPQYLA*TYR*LTWMSFGKLKG RKHCYHFFLFGKSLETGNTRFGRRAELLDKGLM FVQ*LGSVVSF*IFN*NLGLYIPDKYSHLNHSYC KMQKILILLFFALFLNHFLYICIYICVCVCCLC CFVQMWATIATKYILFALKYL*RKYLNVMYMVV IREKSSIINKNEGFCKDFCSAFCKVKF*ASFP*S YVYVSILILPNLPLKSEIPLLSSRLLFSFYSCP AVYPLRMSFLDFSNM*FFFPFRMVILNV*VHVL SYLRYLHPQSAPNSQKLEHCQLICYRSFRNT*LT LKVGCC*FFAKIQILLRDQGDATTP*FSI*KYT CLCKQIFPYP**LFKYLSLKILISHFYTCLNCS QLLHTHQPSTKVVL*KFTTICTFLSQTLVTFALI P*MFLPP*YLYLFFFSLE*RYNCVIFQKYR*MI QKVTVKENHVSLIMNN*LVSLAYIFLIAYDKCF *GNKMRTDKDCVVFWIWREIF*FLNAVTNYNVFI FVLSVKMHDCRIV*ILCLFLMKSFVCSCF*VCT QILRNKSTHVI