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Sequences

Sequence NM_203372

GTCCCAGGCGGTTCCGCTCAACAGACGCTGCTGTGGCTGCGCCGGGCTGCGACACTGCAGTTGTCTACGCGGCCGGGGCCGGGACGAGGAGGCGTTGGAC GGGGTCGCATACGTTCGTCCCCTCGCATTGCGGCCCCGACAGCTGCGCCAGGATCCCCGGGCGGCGGCGCGGGGCGTGAACGCTCTGGGGCTCAGCCAGG CCTGCGCGGGCCCGAGGCCGGAGGAACCCGGACTCCGGCGTAGCGGTTTTGACACAAGGGCGCATATCTTCAAAGCACCTAGTACCTCCTACCATTGTCA ACTGATTCTCGCTGAAGTCTGTTAATTCTACTTTTTGAGTACTTATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATACCATCAA CCCTATTCTTTTATATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTCAAGACAAGAAAAATCA AACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCTGTTAATAGTTTGGATGGTTTGGCTTCAGTATTATACCCTGGATGTG ATACTTTAGATAAAGTTTTTACATATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAGTACAACC AAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTCTTTGTTCGAGCCTTTAATTTTGGAAATGGATTACAG ATGTTGGGTCAGAAACCAAAGACCAACATCGCCATCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTTATGTATAATTTTCAGC TTGTTACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCATGCATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTACAAAC AAAGTTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACATCATCACTGTTGATGGAAAGCCACCGACCTGGTCCGAGTTCCCCAAGGGCATCATT GTGCATACCATGGCTGCAGTGGAGGCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGATATTGCAGTAATCATGT ACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCACATAGTAACATTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAGAACTAGG AGAGGAAGATGTCTACATTGGATATTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTTGTCTGTCTTTCTCACGGATGCCGCATTGGTTACTCT TCACCACAGACTTTAGCAGATCAGTCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCATGTTGAAACCAACACTGATGGCAGCAGTTCCGGAAA TCATGGATCGGATCTACAAAAATGTCATGAATAAAGTCAGTGAAATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAATGGAACA GATTTCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTGCTAGGGGGAAATATTCGTCTCCTGTTGTGTGGTGGC GCTCCACTTTCTGCAACCACGCAGCGATTCATGAACATCTGTTTCTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCTGCTGGGGCTGGAACAA TTTCCGAAGTGTGGGACTACAATACTGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAGGTGGATACTTTAATAC TGATAAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAATGGGGTACTACAAAAATGAAGCAAAAACAAAAGCTGATTTCTTTGAA GATGAAAATGGACAAAGGTGGCTCTGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAAAAGGACCTTGTAAAAC TACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGAATCTTCCACTAGTAGATAACATTTGTGCATATGCAAACAGTTATCATTC TTATGTCATTGGATTTGTTGTGCCAAATCAAAAGGAACTAACTGAACTAGCTCGAAAGAAAGGACTTAAAGGGACTTGGGAGGAGCTGTGTAACAGTTGT GAAATGGAAAATGAGGTACTTAAAGTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCCAGTAAAAATTCGTTTGAGTCCTGAAC CGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTGAAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGTATGGAAG AAAATAATTATTCTCTTCTGGCATCAGTTTGCTACAGTGAGCTCAGATCAAATAGGAAAATACTTGAAATGCATGTCTCAAGCTGCAAGGCAAACTCCAT TCCTCATATTAAACTATTACTTCTCATGACGTCACCATTTTTAACTGACAGGATTAGTAAAACATTAAGACAGCAAACTTGTGTCTGTCTCTTCTTTCAT TTTCCCCGCCACCAACTTACTTTACCACCTATGACTGTACTTGTCAGTATGAGAATTTTTCTGAATCATATTGGGGAAGCAGTGATTTTAAAACCTCAAG TTTTTAAACATGATTTATATGTTCTGTATAATGTTCAGTTTGTAACTTTTTAAAAGTTTGGATGTATAGAGGGATAAATAGGAAATATAAGAATTGGTTA TTTGGGGGCTTTTTTACTTACTGTATTTAAAAATACAAGGGTATTGATATGAAATTATGTAAATTTCAAATGCTTATGAATCAAATCATTGTTGAACAAA AGATTTGTTGCTGTGTAATTATTGTCTTGTATGCATTTGAGAGAAATAAATATACCCATACTTATGTTTTAAGAAGTTGAGATCTTGTGAATATATGCCT GTCAGTGTCTTCTTTATATATTTATTTTTTATTAGAAAAAATGAAGTTTGGTTGGTGATGCATGAAACAAAATAGCAAGAGAGGGTTATAGTTTAATAGT AAGGGAGATAACACAGCATGTGTAGCACCAGTTGATAATTGGTCTCTAGTAGCTTACTGTCAAAATGTTCAATGAAGTCTTCTGTTCATCTGTTGAAACT AGGAAAATACCCAAACTTAAATGGAAGAATTCTGAAAGAGAGGATAGAATTTAAAGAACAAGAGTATATAAAGTTATTCTTTGAATATTTCGTTGACTAT ATGTACATTGAGTTATCTATATTTGTAAACAAATTAGTCATGGAAAATTATTCTATCTCAAAGTCTCCTTTTAGTCTAGATAATCATTATTTCATTTTAA AATTAGTGTTTTTCCTAGTTTGCACTGATGCGTGTATGGATGTGTGTGAGTCAGTGGTAGCTTATTTAAAAAGCACCTTATCCTTTCTCCCATAACCTTT GTACACTAAAAAATGAAAGAATTTAGAATGTATTTGATGATAGCATTCTCACTAAGACACATGAGAATTTAACTTTATAACCGCGTGAGTTAAGATTTAA TTCATAGGTTTTGATGTCATTGTTGAAGTTATTTGTAATTCAGAAACCTTGCTTGTGTGATACATAGTCTCTTCATTTATTACTGCTTGTCTGTTGTTAT ATCTGGATTATCAAAAGCAATAGTGCACCAATTAAGATGTGCTCAAATCAGGACTTAAATCATAGGCACCACATTTTTCATGTCAGACTAGTTACTTTGT TGATTCTCAGTTACTGTAGGCATCAAAAGGCAAAAATCAAAAAAAAAAAAAACAAAAACAAAAAAAAAGATGAACCTAGGTCTGTGTAAAGTAAGGGGAG TGTTAGGAGCAGCCAGGACTGTGTAGTGTGTGTTTGGTTGCATCACAAACATCGTATGTGGAGACATTGCAATACAGTGTTTTTTGTTTTCAACTTTTCT TGTATTGTATATTTGTATTATGTTTTGAATGCTTTTCTCTTTTCATAATTAAATATTAATGTTTGGGATAACTGCCAAGAAGAAGTAAAAATATTGAATG GAACTTCTATATGAGGATGCTGTGATCTAAAAATTAAATCTCAGTGGGCGGAGA

refseq_ACSL3.F1 (forward primer)
refseq_ACSL3.R1 (reverse primer)
Single exon skipping

Frame 0

VPGGSAQQTLLWLRRAATLQLSTRPGPGRGGVG RGRIRSSPRIAAPTAAPGSPGGGAGRERSGAQPG LRGPEAGGTRTPA*RF*HKGAYLQST*YLLPLS TDSR*SLLILLFEYL*ITTCLQNHLP*S*NIPS TLFFYILYIF*YHFILF*HTFRFIFSPSQDKKNQ TELKQSL*IQNLILHTDLLIVWMVWLQYYTLDV IL*IKFLHMQKTNLRTKDSWEHVKF*MRKMKYN QMEKFLKRLFLDSIIGFPMKMSLFEPLILEMDYR CWVRNQRPTSPSSVRPGPSG**LHRRVLCIIFS LLHYMPL*EVQPLFMH*MKQR*PTSLLVKNSYK QS*RI*FLWSHACGTSSLLMESHRPGPSSPRASL CIPWLQWRPWEPRPAWKTNLIANHCPQILQ*SC TQVDPQDFQRES*SHIVTLLLV*LGWQKGFQN* ERKMSTLDICLWPMF*N*VLSLSVFLTDAALVTL HHRL*QISLQKLKKEAKGIHPC*NQH*WQQFRK SWIGSTKMS*IKSVK*VVFNVICLFWPIITKWN RFQKDVILHCATALFSGKFEAC*GEIFVSCCVVA LHFLQPRSDS*TSVSAVLLVRDTGSLNLLGLEQ FPKCGTTILAEWEHH*FAVKSN*KTGRKVDTLI LISHTPGVKFLLGAKV*QWGTTKMKQKQKLISLK MKMDKGGSVLGILESLNPMDA*RLLIVKRTL*N YRQGNMFLLGK*RQL*RIFH**ITFVHMQTVII LMSLDLLCQIKRN*LN*LERKDLKGLGRSCVTVV KWKMRYLKCFPKLLFQQVWKSLKFQ*KFV*VLN RGPLKLVW*QMPSS*NAKSLKHITRRTLSECME ENNYSLLASVCYSELRSNRKILEMHVSSCKANSI PHIKLLLLMTSPFLTDRISKTLRQQTCVCLFFH FPRHQLTLPPMTVLVSMRIFLNHIGEAVILKPQ VFKHDLYVLYNVQFVTF*KFGCIEG*IGNIRIGY LGAFLLTVFKNTRVLI*NYVNFKCL*IKSLLNK RFVAV*LLSCMHLREINIPILMF*EVEIL*IYA CQCLLYIFIFY*KK*SLVGDA*NKIAREGYSLIV REITQHV*HQLIIGL**LTVKMFNEVFCSSVET RKIPKLKWKNSEREDRI*RTRVYKVIL*IFR*L YVH*VIYICKQISHGKLFYLKVSF*SR*SLFHFK ISVFPSLH*CVYGCV*VSGSLFKKHLILSPITF VH*KMKEFRMYLMIAFSLRHMRI*LYNRVS*DL IHRF*CHC*SYL*FRNLACVIHSLFIYYCLSVVI SGLSKAIVHQLRCAQIRT*IIGTTFFMSD*LLC *FSVTVGIKRQKSKKKKTKTKKKMNLGLCKVRG VLGAARTV*CVFGCITNIVCGDIAIQCFLFSTFL VLYICIMF*MLFSFHN*ILMFGITAKKK*KY*M ELLYEDAVI*KLNLSGRR

Frame 1

SQAVPLNRRCCGCAGLRHCSCLRGRGRDEEALD GVAYVRPLALRPRQLRQDPRAAARGVNALGLSQ ACAGPRPEEPGLRRSGFDTRAHIFKAPSTSYHCQ LILAEVC*FYFLSTYE*PRVFKTIYHEAKTYHQ PYSFIFYTFSNITLYYFNIHSVLFFLRVKTRKI KPN*SKACKFKT*FCIQIC**FGWFGFSIIPWM* YFR*SFYICKKQI*EQKTLGNT*SFK*GR*STT KWKNF*KGYSWTV*LAFL*RCLCSSL*FWKWIT DVGSETKDQHRHLL*DQGRVDDSCTGVFYV*FSA CYIICHSRRSSHCSCIK*NRGDQHHY**RTLTN KVEGYSFFGPTPAAHHHC*WKATDLVRVPQGHH CAYHGCSGGPGSQGQHGKPTS*QTIALRYCSNHV HKWIHRTSKGSHDLT**HYCWYNWDGRKDSRTR RGRCLHWIFASGPCSRIKC*ACLSFSRMPHWLL FTTDFSRSVFKN*KRKQRGYIHVETNTDGSSSGN HGSDLQKCHE*SQ*NE*FST*SVYSGL*LQNGT DFKRT*YSTVRQLCFPESSKLARGKYSSPVVWW RSTFCNHAAIHEHLFLLSCWSGIRAH*ICWGWNN FRSVGLQYWQSGSTISLL*NQIKKLGGRWIL*Y **ATPQG*NSYWGPKCDNGVLQK*SKNKS*FL* R*KWTKVALYWGYWRV*TRWMLKDY*S*KGPCKT TGRGICFSWESRGSFEESSTSR*HLCICKQLSF LCHWICCAKSKGTN*TSSKERT*RDLGGAV*QL *NGK*GT*SAFRSCYFSKSGKV*NSSKNSFES*T VDP*NWSGDRCLQAETQRA*NTLPGGH*ANVWK KIIILFWHQFATVSSDQIGKYLKCMSQAARQTP FLILNYYFS*RHHF*LTGLVKH*DSKLVSVSSFI FPATNLLYHL*LYLSV*EFF*IILGKQ*F*NLK FLNMIYMFCIMFSL*LFKSLDV*RDK*EI*ELV IWGLFYLLYLKIQGY*YEIM*ISNAYESNHC*TK DLLLCNYCLVCI*EK*IYPYLCFKKLRSCEYMP VSVFFIYLFFIRKNEVWLVMHETK*QERVIV** *GR*HSMCSTS**LVSSSLLSKCSMKSSVHLLKL GKYPNLNGRILKERIEFKEQEYIKLFFEYFVDY MYIELSIFVNKLVMENYSISKSPFSLDNHYFIL KLVFFLVCTDACMDVCESVVAYLKSTLSFLP*PL YTKK*KNLECI***HSH*DT*EFNFITA*VKI* FIGFDVIVEVICNSETLLV*YIVSSFITACLLL YLDYQKQ*CTN*DVLKSGLKS*APHFSCQTSYFV DSQLL*ASKGKNQKKKKQKQKKR*T*VCVK*GE C*EQPGLCSVCLVASQTSYVETLQYSVFCFQLF LYCIFVLCFECFSLFIIKY*CLG*LPRRSKNIEW NFYMRML*SKN*ISVGG

Frame 2

PRRFRSTDAAVAAPGCDTAVVYAAGAGTRRRWT GSHTFVPSHCGPDSCARIPGRRRGA*TLWGSAR PARARGRRNPDSGVAVLTQGRISSKHLVPPTIV N*FSLKSVNSTF*VLMNNHVSSKPSTMKLKHTIN PILLYFIHFLISLYTILTYIPFYFFSESRQEKS NRIKAKPVNSKPDSAYRSVNSLDGLASVLYPGC DTLDKVFTYAKNKFKNKRLLGTREVLNEEDEVQP NGKIFKKVILGQYNWLSYEDVFVRAFNFGNGLQ MLGQKPKTNIAIFCETRAEWMIAAQACFMYNFQ LVTLYATLGGPAIVHALNETEVTNIITSKELLQT KLKDIVSLVPRLRHIITVDGKPPTWSEFPKGII VHTMAAVEALGAKASMENQPHSKPLPSDIAVIM YTSGSTGLPKGVMISHSNIIAGITGMAERIPELG EEDVYIGYLPLAHVLELSAELVCLSHGCRIGYS SPQTLADQSSKIKKGSKGDTSMLKPTLMAAVPE IMDRIYKNVMNKVSEMSSFQRNLFILAYNYKMEQ ISKGRNTPLCDSFVFRKVRSLLGGNIRLLLCGG APLSATTQRFMNICFCCPVGQGYGLTESAGAGT ISEVWDYNTGRVGAPLVCCEIKLKNWEEGGYFNT DKPHPRGEILIGGQSVTMGYYKNEAKTKADFFE DENGQRWLCTGDIGEFEPDGCLKIIDRKKDLVK LQAGEYVSLGKVEAALKNLPLVDNICAYANSYHS YVIGFVVPNQKELTELARKKGLKGTWEELCNSC EMENEVLKVLSEAAISASLEKFEIPVKIRLSPE PWTPETGLVTDAFKLKRKELKTHYQADIERMYGR K*LFSSGISLLQ*AQIK*ENT*NACLKLQGKLH SSY*TITSHDVTIFN*QD**NIKTANLCLSLLS FSPPPTYFTTYDCTCQYENFSESYWGSSDFKTSS F*T*FICSV*CSVCNFLKVWMYRGINRKYKNWL FGGFFTYCI*KYKGIDMKLCKFQMLMNQIIVEQ KICCCVIIVLYAFERNKYTHTYVLRS*DLVNICL SVSSLYIYFLLEKMKFGW*CMKQNSKRGL*FNS KGDNTACVAPVDNWSLVAYCQNVQ*SLLFIC*N *ENTQT*MEEF*KRG*NLKNKSI*SYSLNISLTI CTLSYLYL*TN*SWKIILSQSLLLV*IIIISF* N*CFS*FALMRVWMCVSQW*LI*KAPYPFSHNL CTLKNERI*NVFDDSILTKTHENLTL*PRELRFN S*VLMSLLKLFVIQKPCLCDT*SLHLLLLVCCY IWIIKSNSAPIKMCSNQDLNHRHHIFHVRLVTL LILSYCRHQKAKIKKKKNKNKKKDEPRSV*SKGS VRSSQDCVVCVWLHHKHRMWRHCNTVFFVFNFS CIVYLYYVLNAFLFS*LNINVWDNCQEEVKILN GTSI*GCCDLKIKSQWAE

Sequence NM_004457

GTCCCAGGCGGTTCCGCTCAACAGACGCTGCTGTGGCTGCGCCGGGCTGCGACACTGCAGTTGTCTACGCGGCCGGGGCCGGGACGAGGAGGCGTTGGAC GGGGTCGCATACGTTCGTCCCCTCGCATTGCGGCCCCGACAGCTGCGCCAGGATCCCCGGGCGGCGGCGCGGGGCGTGAACGCTCTGGGGCTCAGCCAGG CCTGCGCGGGCCCGAGGCCGGAGGAACCCGGACTCCGGCGTAGCGGTTTTGACACAAGGGCGCATATCTTCAAAGCACCTAGTACCTCCTACCATTGTCA ACTGATACAGAATTCGTTGTTGGGAAGGACTGGGGAAACAGCTGTAACATTTGCCACCCTCAGAAGCTGCTGGTCCTGTGTCACACCACCTTAGCCTCTT GATCGAGGAAGATTCTCGCTGAAGTCTGTTAATTCTACTTTTTGAGTACTTATGAATAACCACGTGTCTTCAAAACCATCTACCATGAAGCTAAAACATA CCATCAACCCTATTCTTTTATATTTTATACATTTTCTAATATCACTTTATACTATTTTAACATACATTCCGTTTTATTTTTTCTCCGAGTCAAGACAAGA AAAATCAAACCGAATTAAAGCAAAGCCTGTAAATTCAAAACCTGATTCTGCATACAGATCTGTTAATAGTTTGGATGGTTTGGCTTCAGTATTATACCCT GGATGTGATACTTTAGATAAAGTTTTTACATATGCAAAAAACAAATTTAAGAACAAAAGACTCTTGGGAACACGTGAAGTTTTAAATGAGGAAGATGAAG TACAACCAAATGGAAAAATTTTTAAAAAGGTTATTCTTGGACAGTATAATTGGCTTTCCTATGAAGATGTCTTTGTTCGAGCCTTTAATTTTGGAAATGG ATTACAGATGTTGGGTCAGAAACCAAAGACCAACATCGCCATCTTCTGTGAGACCAGGGCCGAGTGGATGATAGCTGCACAGGCGTGTTTTATGTATAAT TTTCAGCTTGTTACATTATATGCCACTCTAGGAGGTCCAGCCATTGTTCATGCATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCT TACAAACAAAGTTGAAGGATATAGTTTCTTTGGTCCCACGCCTGCGGCACATCATCACTGTTGATGGAAAGCCACCGACCTGGTCCGAGTTCCCCAAGGG CATCATTGTGCATACCATGGCTGCAGTGGAGGCCCTGGGAGCCAAGGCCAGCATGGAAAACCAACCTCATAGCAAACCATTGCCCTCAGATATTGCAGTA ATCATGTACACAAGTGGATCCACAGGACTTCCAAAGGGAGTCATGATCTCACATAGTAACATTATTGCTGGTATAACTGGGATGGCAGAAAGGATTCCAG AACTAGGAGAGGAAGATGTCTACATTGGATATTTGCCTCTGGCCCATGTTCTAGAATTAAGTGCTGAGCTTGTCTGTCTTTCTCACGGATGCCGCATTGG TTACTCTTCACCACAGACTTTAGCAGATCAGTCTTCAAAAATTAAAAAAGGAAGCAAAGGGGATACATCCATGTTGAAACCAACACTGATGGCAGCAGTT CCGGAAATCATGGATCGGATCTACAAAAATGTCATGAATAAAGTCAGTGAAATGAGTAGTTTTCAACGTAATCTGTTTATTCTGGCCTATAATTACAAAA TGGAACAGATTTCAAAAGGACGTAATACTCCACTGTGCGACAGCTTTGTTTTCCGGAAAGTTCGAAGCTTGCTAGGGGGAAATATTCGTCTCCTGTTGTG TGGTGGCGCTCCACTTTCTGCAACCACGCAGCGATTCATGAACATCTGTTTCTGCTGTCCTGTTGGTCAGGGATACGGGCTCACTGAATCTGCTGGGGCT GGAACAATTTCCGAAGTGTGGGACTACAATACTGGCAGAGTGGGAGCACCATTAGTTTGCTGTGAAATCAAATTAAAAAACTGGGAGGAAGGTGGATACT TTAATACTGATAAGCCACACCCCAGGGGTGAAATTCTTATTGGGGGCCAAAGTGTGACAATGGGGTACTACAAAAATGAAGCAAAAACAAAAGCTGATTT CTTTGAAGATGAAAATGGACAAAGGTGGCTCTGTACTGGGGATATTGGAGAGTTTGAACCCGATGGATGCTTAAAGATTATTGATCGTAAAAAGGACCTT GTAAAACTACAGGCAGGGGAATATGTTTCTCTTGGGAAAGTAGAGGCAGCTTTGAAGAATCTTCCACTAGTAGATAACATTTGTGCATATGCAAACAGTT ATCATTCTTATGTCATTGGATTTGTTGTGCCAAATCAAAAGGAACTAACTGAACTAGCTCGAAAGAAAGGACTTAAAGGGACTTGGGAGGAGCTGTGTAA CAGTTGTGAAATGGAAAATGAGGTACTTAAAGTGCTTTCCGAAGCTGCTATTTCAGCAAGTCTGGAAAAGTTTGAAATTCCAGTAAAAATTCGTTTGAGT CCTGAACCGTGGACCCCTGAAACTGGTCTGGTGACAGATGCCTTCAAGCTGAAACGCAAAGAGCTTAAAACACATTACCAGGCGGACATTGAGCGAATGT ATGGAAGAAAATAATTATTCTCTTCTGGCATCAGTTTGCTACAGTGAGCTCAGATCAAATAGGAAAATACTTGAAATGCATGTCTCAAGCTGCAAGGCAA ACTCCATTCCTCATATTAAACTATTACTTCTCATGACGTCACCATTTTTAACTGACAGGATTAGTAAAACATTAAGACAGCAAACTTGTGTCTGTCTCTT CTTTCATTTTCCCCGCCACCAACTTACTTTACCACCTATGACTGTACTTGTCAGTATGAGAATTTTTCTGAATCATATTGGGGAAGCAGTGATTTTAAAA CCTCAAGTTTTTAAACATGATTTATATGTTCTGTATAATGTTCAGTTTGTAACTTTTTAAAAGTTTGGATGTATAGAGGGATAAATAGGAAATATAAGAA TTGGTTATTTGGGGGCTTTTTTACTTACTGTATTTAAAAATACAAGGGTATTGATATGAAATTATGTAAATTTCAAATGCTTATGAATCAAATCATTGTT GAACAAAAGATTTGTTGCTGTGTAATTATTGTCTTGTATGCATTTGAGAGAAATAAATATACCCATACTTATGTTTTAAGAAGTTGAGATCTTGTGAATA TATGCCTGTCAGTGTCTTCTTTATATATTTATTTTTTATTAGAAAAAATGAAGTTTGGTTGGTGATGCATGAAACAAAATAGCAAGAGAGGGTTATAGTT TAATAGTAAGGGAGATAACACAGCATGTGTAGCACCAGTTGATAATTGGTCTCTAGTAGCTTACTGTCAAAATGTTCAATGAAGTCTTCTGTTCATCTGT TGAAACTAGGAAAATACCCAAACTTAAATGGAAGAATTCTGAAAGAGAGGATAGAATTTAAAGAACAAGAGTATATAAAGTTATTCTTTGAATATTTCGT TGACTATATGTACATTGAGTTATCTATATTTGTAAACAAATTAGTCATGGAAAATTATTCTATCTCAAAGTCTCCTTTTAGTCTAGATAATCATTATTTC ATTTTAAAATTAGTGTTTTTCCTAGTTTGCACTGATGCGTGTATGGATGTGTGTGAGTCAGTGGTAGCTTATTTAAAAAGCACCTTATCCTTTCTCCCAT AACCTTTGTACACTAAAAAATGAAAGAATTTAGAATGTATTTGATGATAGCATTCTCACTAAGACACATGAGAATTTAACTTTATAACCGCGTGAGTTAA GATTTAATTCATAGGTTTTGATGTCATTGTTGAAGTTATTTGTAATTCAGAAACCTTGCTTGTGTGATACATAGTCTCTTCATTTATTACTGCTTGTCTG TTGTTATATCTGGATTATCAAAAGCAATAGTGCACCAATTAAGATGTGCTCAAATCAGGACTTAAATCATAGGCACCACATTTTTCATGTCAGACTAGTT ACTTTGTTGATTCTCAGTTACTGTAGGCATCAAAAGGCAAAAATCAAAAAAAAAAAAAACAAAAACAAAAAAAAAGATGAACCTAGGTCTGTGTAAAGTA AGGGGAGTGTTAGGAGCAGCCAGGACTGTGTAGTGTGTGTTTGGTTGCATCACAAACATCGTATGTGGAGACATTGCAATACAGTGTTTTTTGTTTTCAA CTTTTCTTGTATTGTATATTTGTATTATGTTTTGAATGCTTTTCTCTTTTCATAATTAAATATTAATGTTTGGGATAACTGCCAAGAAGAAGTAAAAATA TTGAATGGAACTTCTATATGAGGATGCTGTGATCTAAAAATTAAATCTCAGTGGGCGGAGA

refseq_ACSL3.F1 (forward primer)
refseq_ACSL3.R1 (reverse primer)
Single exon skipping

Frame 0

VPGGSAQQTLLWLRRAATLQLSTRPGPGRGGVG RGRIRSSPRIAAPTAAPGSPGGGAGRERSGAQPG LRGPEAGGTRTPA*RF*HKGAYLQST*YLLPLS TDTEFVVGKDWGNSCNICHPQKLLVLCHTTLAS *SRKILAEVC*FYFLSTYE*PRVFKTIYHEAKTY HQPYSFIFYTFSNITLYYFNIHSVLFFLRVKTR KIKPN*SKACKFKT*FCIQIC**FGWFGFSIIP WM*YFR*SFYICKKQI*EQKTLGNT*SFK*GR*S TTKWKNF*KGYSWTV*LAFL*RCLCSSL*FWKW ITDVGSETKDQHRHLL*DQGRVDDSCTGVFYV* FSACYIICHSRRSSHCSCIK*NRGDQHHY**RTL TNKVEGYSFFGPTPAAHHHC*WKATDLVRVPQG HHCAYHGCSGGPGSQGQHGKPTS*QTIALRYCS NHVHKWIHRTSKGSHDLT**HYCWYNWDGRKDSR TRRGRCLHWIFASGPCSRIKC*ACLSFSRMPHW LLFTTDFSRSVFKN*KRKQRGYIHVETNTDGSS SGNHGSDLQKCHE*SQ*NE*FST*SVYSGL*LQN GTDFKRT*YSTVRQLCFPESSKLARGKYSSPVV WWRSTFCNHAAIHEHLFLLSCWSGIRAH*ICWG WNNFRSVGLQYWQSGSTISLL*NQIKKLGGRWIL *Y**ATPQG*NSYWGPKCDNGVLQK*SKNKS*F L*R*KWTKVALYWGYWRV*TRWMLKDY*S*KGP CKTTGRGICFSWESRGSFEESSTSR*HLCICKQL SFLCHWICCAKSKGTN*TSSKERT*RDLGGAV* QL*NGK*GT*SAFRSCYFSKSGKV*NSSKNSFE S*TVDP*NWSGDRCLQAETQRA*NTLPGGH*ANV WKKIIILFWHQFATVSSDQIGKYLKCMSQAARQ TPFLILNYYFS*RHHF*LTGLVKH*DSKLVSVS SFIFPATNLLYHL*LYLSV*EFF*IILGKQ*F*N LKFLNMIYMFCIMFSL*LFKSLDV*RDK*EI*E LVIWGLFYLLYLKIQGY*YEIM*ISNAYESNHC *TKDLLLCNYCLVCI*EK*IYPYLCFKKLRSCEY MPVSVFFIYLFFIRKNEVWLVMHETK*QERVIV ***GR*HSMCSTS**LVSSSLLSKCSMKSSVHL LKLGKYPNLNGRILKERIEFKEQEYIKLFFEYFV DYMYIELSIFVNKLVMENYSISKSPFSLDNHYF ILKLVFFLVCTDACMDVCESVVAYLKSTLSFLP *PLYTKK*KNLECI***HSH*DT*EFNFITA*VK I*FIGFDVIVEVICNSETLLV*YIVSSFITACL LLYLDYQKQ*CTN*DVLKSGLKS*APHFSCQTS YFVDSQLL*ASKGKNQKKKKQKQKKR*T*VCVK* GEC*EQPGLCSVCLVASQTSYVETLQYSVFCFQ LFLYCIFVLCFECFSLFIIKY*CLG*LPRRSKN IEWNFYMRML*SKN*ISVGG

Frame 1

SQAVPLNRRCCGCAGLRHCSCLRGRGRDEEALD GVAYVRPLALRPRQLRQDPRAAARGVNALGLSQ ACAGPRPEEPGLRRSGFDTRAHIFKAPSTSYHCQ LIQNSLLGRTGETAVTFATLRSCWSCVTPP*PL DRGRFSLKSVNSTF*VLMNNHVSSKPSTMKLKH TINPILLYFIHFLISLYTILTYIPFYFFSESRQE KSNRIKAKPVNSKPDSAYRSVNSLDGLASVLYP GCDTLDKVFTYAKNKFKNKRLLGTREVLNEEDE VQPNGKIFKKVILGQYNWLSYEDVFVRAFNFGNG LQMLGQKPKTNIAIFCETRAEWMIAAQACFMYN FQLVTLYATLGGPAIVHALNETEVTNIITSKEL LQTKLKDIVSLVPRLRHIITVDGKPPTWSEFPKG IIVHTMAAVEALGAKASMENQPHSKPLPSDIAV IMYTSGSTGLPKGVMISHSNIIAGITGMAERIP ELGEEDVYIGYLPLAHVLELSAELVCLSHGCRIG YSSPQTLADQSSKIKKGSKGDTSMLKPTLMAAV PEIMDRIYKNVMNKVSEMSSFQRNLFILAYNYK MEQISKGRNTPLCDSFVFRKVRSLLGGNIRLLLC GGAPLSATTQRFMNICFCCPVGQGYGLTESAGA GTISEVWDYNTGRVGAPLVCCEIKLKNWEEGGY FNTDKPHPRGEILIGGQSVTMGYYKNEAKTKADF FEDENGQRWLCTGDIGEFEPDGCLKIIDRKKDL VKLQAGEYVSLGKVEAALKNLPLVDNICAYANS YHSYVIGFVVPNQKELTELARKKGLKGTWEELCN SCEMENEVLKVLSEAAISASLEKFEIPVKIRLS PEPWTPETGLVTDAFKLKRKELKTHYQADIERM YGRK*LFSSGISLLQ*AQIK*ENT*NACLKLQGK LHSSY*TITSHDVTIFN*QD**NIKTANLCLSL LSFSPPPTYFTTYDCTCQYENFSESYWGSSDFK TSSF*T*FICSV*CSVCNFLKVWMYRGINRKYKN WLFGGFFTYCI*KYKGIDMKLCKFQMLMNQIIV EQKICCCVIIVLYAFERNKYTHTYVLRS*DLVN ICLSVSSLYIYFLLEKMKFGW*CMKQNSKRGL*F NSKGDNTACVAPVDNWSLVAYCQNVQ*SLLFIC *N*ENTQT*MEEF*KRG*NLKNKSI*SYSLNIS LTICTLSYLYL*TN*SWKIILSQSLLLV*IIIIS F*N*CFS*FALMRVWMCVSQW*LI*KAPYPFSH NLCTLKNERI*NVFDDSILTKTHENLTL*PREL RFNS*VLMSLLKLFVIQKPCLCDT*SLHLLLLVC CYIWIIKSNSAPIKMCSNQDLNHRHHIFHVRLV TLLILSYCRHQKAKIKKKKNKNKKKDEPRSV*S KGSVRSSQDCVVCVWLHHKHRMWRHCNTVFFVFN FSCIVYLYYVLNAFLFS*LNINVWDNCQEEVKI LNGTSI*GCCDLKIKSQWAE

Frame 2

PRRFRSTDAAVAAPGCDTAVVYAAGAGTRRRWT GSHTFVPSHCGPDSCARIPGRRRGA*TLWGSAR PARARGRRNPDSGVAVLTQGRISSKHLVPPTIV N*YRIRCWEGLGKQL*HLPPSEAAGPVSHHLSLL IEEDSR*SLLILLFEYL*ITTCLQNHLP*S*NI PSTLFFYILYIF*YHFILF*HTFRFIFSPSQDK KNQTELKQSL*IQNLILHTDLLIVWMVWLQYYTL DVIL*IKFLHMQKTNLRTKDSWEHVKF*MRKMK YNQMEKFLKRLFLDSIIGFPMKMSLFEPLILEM DYRCWVRNQRPTSPSSVRPGPSG**LHRRVLCII FSLLHYMPL*EVQPLFMH*MKQR*PTSLLVKNS YKQS*RI*FLWSHACGTSSLLMESHRPGPSSPR ASLCIPWLQWRPWEPRPAWKTNLIANHCPQILQ* SCTQVDPQDFQRES*SHIVTLLLV*LGWQKGFQ N*ERKMSTLDICLWPMF*N*VLSLSVFLTDAAL VTLHHRL*QISLQKLKKEAKGIHPC*NQH*WQQF RKSWIGSTKMS*IKSVK*VVFNVICLFWPIITK WNRFQKDVILHCATALFSGKFEAC*GEIFVSCC VVALHFLQPRSDS*TSVSAVLLVRDTGSLNLLGL EQFPKCGTTILAEWEHH*FAVKSN*KTGRKVDT LILISHTPGVKFLLGAKV*QWGTTKMKQKQKLI SLKMKMDKGGSVLGILESLNPMDA*RLLIVKRTL *NYRQGNMFLLGK*RQL*RIFH**ITFVHMQTV IILMSLDLLCQIKRN*LN*LERKDLKGLGRSCV TVVKWKMRYLKCFPKLLFQQVWKSLKFQ*KFV*V LNRGPLKLVW*QMPSS*NAKSLKHITRRTLSEC MEENNYSLLASVCYSELRSNRKILEMHVSSCKA NSIPHIKLLLLMTSPFLTDRISKTLRQQTCVCLF FHFPRHQLTLPPMTVLVSMRIFLNHIGEAVILK PQVFKHDLYVLYNVQFVTF*KFGCIEG*IGNIR IGYLGAFLLTVFKNTRVLI*NYVNFKCL*IKSLL NKRFVAV*LLSCMHLREINIPILMF*EVEIL*I YACQCLLYIFIFY*KK*SLVGDA*NKIAREGYS LIVREITQHV*HQLIIGL**LTVKMFNEVFCSSV ETRKIPKLKWKNSEREDRI*RTRVYKVIL*IFR *LYVH*VIYICKQISHGKLFYLKVSF*SR*SLF HFKISVFPSLH*CVYGCV*VSGSLFKKHLILSPI TFVH*KMKEFRMYLMIAFSLRHMRI*LYNRVS* DLIHRF*CHC*SYL*FRNLACVIHSLFIYYCLS VVISGLSKAIVHQLRCAQIRT*IIGTTFFMSD*L LC*FSVTVGIKRQKSKKKKTKTKKKMNLGLCKV RGVLGAARTV*CVFGCITNIVCGDIAIQCFLFS TFLVLYICIMF*MLFSFHN*ILMFGITAKKK*KY *MELLYEDAVI*KLNLSGRR