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Sequences

Sequence NM_014776

GAAGCGCCGCCGCCACCGCCGCCTTTGCTGTCCCCCGGCCTCTAGTTCCCCGCAGGTGGGAGGTGGGAGCCATGTCGAAACGGCTCCGGAGCAGCGAGGT GTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGC CATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGG AGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAA GTATCAGATGTTAGCGTTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGCGTGAGAACA GGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAG CAGGGCAGATTTTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCAAGGCAAGG AGGGCACCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGATCACAAAAAT GGACAGCACTTTATAATACCTCAAATGGCAGACAGCAGCCTGGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATT TGTTTGAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTTGCCACGCAAAACCACAGCGCCCTGGTAACCGA GACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCC ACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCA ATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAAC AAACCGGCAGAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGAAACAGGCCACAACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACA GACACTTCCAACCACTCTTCCTTAAAGAGACGTCCGTCTGCCCGGGGCAGTAGGCCCATGTCCATGTACGAGACCGGATCAGGTCAGAAACCATATCTCC CAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGGATGAGACTCCAGCCCTTCCCCGCGCACGCATCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAG TGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGGACGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTA CCAGACACAGCAGAACCCCATGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTCATCAGGAAGACTGAACAGATCACCAAAAACATACAGGAGC TCTTAAGAGCAGCCCAAGAAAATAAACATGACAGTTATATTCCCTGCTCAGAGAGGATACACGTAGCTGTTACAGAAATGGCAGCATTATTCCCCAAAAA ACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGACTGCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGC TCACCCACAGACGTTCAGCTGGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCCAAGGCTGCCAAGCAGCTGGTTACCATCACCACCAAAGAGA ACAACAACTGACAAGGGCAGGGCACCGCCTCCTCTGTTTTCTAGGCTTTATAAAGTCCAATTTCAAATTCAGACGCAGAACTCTTCAGATTTCTACAAAA AGAAACATTTAATGACGGTTTAAAACTTTTTAAAAGAAAACTCAGTATTATTTTTGCATGTTTTCTAACCAATTTTCAACTATTTAACCCACTTGCCTTA TTTTGTGCCTATTTGCTGGTTTAGTTTCTGATGTTCGGTTGTGTTGTCAACAACTGTTGAGTTGATCACATACATCCAAAAGTATAGTTTCATTGTCTAA AGTTTGTGAAAACTTTTCCATCCCTTAAAACTCCCTGCCTATGGCTGAAACTATAAATGAAATTTTTCATAAAACATTTTCTTGAGAGAGTTCAGTTAAA AAAGAAAAAATCAGCGTCATTTGTTCCCTGCCAAGCATCGTGCAATAAGTGAATTTCCTGGCCTACTTCATAATCTCTAAGTTTGGGGTATCTCCAAGCA TGTATGTAGAGAAAGGACTTTGCCGCTTTTTAAAACTAGTGTCACAGTCTCTCTTGGGAGGAAACAATGAATTGGTTCATTTCTTCTCAGTGTTTTACCC TGTTACATGTTCTGTTATTTAATGTTGTTATCTCAAGCTCCTATCTCTTGATGTTTTCTTGTCATCTTGAAGGCTCCATGGAATGAGATAAAGCATTGAC CCACCACAGGACATCTCATGAAGCCATGTTCAGGGTAGGAGCTCCACGCACATCACCACCCATTGTCAGGTGCTCTCCCTCACCCCTGTAAGCCAGACAG TTTGTTCAAAACCTGCAGCAAAGAGAAGCATCAGTCTAACAAGCCAGAACTCATTTTGTGCCACCCATTTAGGTATCACTACCATGAACTATAGGGAAAA GAAATCACCTACTCCCTGACACTGTGTTGTACCCTTCATCCAAAAGTCAGCTTGCTTCTCTGACCACTTACAGCTTTCTCACATGAGTTGCTTCACATTT TTCATCATCTTTAAGGACTTCTTATAAGGAAAGATGTATCGTATCGCAGTCATCTGAGTTAGTTTCTCCTCCATCACCTCATGAGTACTTAAAAATTAGA CATCAACTTTCAAGAGTTCCTTAGAGAGTGTGCTCTAGCTGGCTGCCTGCTACCCCAAAGCTCTGAAAGAGTAAGGTTGAAGTGCTGGAACATGCTCAGA TTGTTTTTTAGTTACTCAAAAGCCCCTTCTTGTTCTGAATGTGAAAGCTCTTCCCGTTCCTGCTTACTGCAGCCATTCTGTGTGTGTGGTTCAGACCCTG GCCTAGGGCCGGTGATGTGGATGAGTGAAGGGAGCCAGGTACACGGCAGCAGGGCAGAGGAGACTATGGGGAGCCCACCGCCTACACAGTAGGGGCAGTA GCCCCCAGCTCCACGCCTGTTGCCATGTGGAAAAGTGGGCCTAGGGTGGCCACAGCTTTGATTTTTCAAGAGACAAGAAACCTAGATGTTTTTTGGTGCC ATCTCTCCATTTATAAATGTTGGCAACCAATTTGAACACTTTCAGAGACCATGTGCAGGTGAGCTGTGTCCTCTAGGCTGCCAGCTCACAGCCCCCACCC CAGGGCCCTGTAAGCTGTGCAGTGTGAGGGCCTGGCTTCCCCGGACACACCTGGAGCAGCCTCTGAGTCTTACCCGTAATGGAGCCGGCCAACCCTGTGT GTTATGTTGCTGTCGCTCCTGTTTTTAACCATATGTTCTGTCCCATAGCAGTCATTCACACATGTGAAGAAGACACAAGAATCAAAGTACAAGTTGCCTC AATACATGCTAATAACTAAGCTTCTATTTTCACAGGACTGATCGATGCACACCTTTATAGCTTGACCATATGCTGTCATGAAAGTAGCACCCCGACGGTG CTTGCTTTACTAAGTCACCGTGTGTGGATGCTTTTGTCGCATTTTGCTTCACTGTGAGTAGCATGGGAAAAGGGTCCACGTCTCCAAAAGACTCTTCCAC TTGTTTGTCCCACAGCCCTAAGTGCCACATTGCTGTTGGATGCTCCCGGGATGCCTTCTAGAAGGGGGCCTCCCACCTTAAGTTCAGGCGCCATCTGATA GCCTTTTCTGCAGGCTTCATGTCCTTAGCTGACCCTCCTTTATCTTGCAAACCCCTGCCCCTGCTGTCCCAGGGAGGACGGGCTTCTGTAGAGGCTTCTG GGAAATGAGCTGTCTGCAGGAGGTGAAGCAGTGTTTTCCTTGGAATGTTTGAGGACCTGCAATAGAAGATGTTGCCTTTATGGCCCTAGAGAAAGTCAAG GAACATGGGGACCAACAAAGCATTTCCAGTTGTAAGAATATGTCAGAAGAGTGCTCGGTTAGCCCACGGTGCCAAAACTCCAAACAAGTTCCCCGGTGGA ATGACGTGGCCATTGGAGGGCTGCAGGTCGTCCTCTCTGTATGTGCCCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGTGTCAGAGTTCTCTGCTCCT GTTGGCAAATCGCACCTTATCTGAACATTTGTTCCAGCCACCTTTTGCACATGGATTCCGTACCTTGCACTAGCAATGTCTACCTTCAGTAACGTCGTTC TTTTTCACCCTGTATTCTCAATGATCCCTTCCTCCCCATATGCCACCCTTCCCCCAAAGCTGATAGGGCAGAGGTGCACTTTATGAAACTGGCACTTGAC TGAACAGAAGGAAACAGCTGTACCTCTAAGCTCCTTTCACAGAAATCGTCATCTGCCTTGGAATGCAAATAGTTCTTATTTGCCAAAGAACAGTGAATTG GGCCCAAAGATCAAACACAGCATTTCATGTAATCAGAATTCTAAACGCACAACTCGGGAGCTGTGCACGGCTTCTCAAGTGATTCAGTTGCAGAACCAAG AGCAAAACTCGCCTGTTTTTGTCCTCAATGGCACCCATGAGCTTTCCAATAGTATAGTTTTCTTTCTTTCCTTGTCCTGGTTGAAGGTAACGTGTTCCTA GAAAAGAAAATGCTGCATAGGAAAACTGAGATGCCTTTTATTCCAACGTGTTTGATCCCTGTCACTCATTATTCTGTACTTTTTATTGTACATTCTATTC CTGTAATTATTGTGCCCTTTAAGTGTTGTAAAATCGTTTTGGAATTTGTGCCTGCTAGTTATGCAATAAACAGGCTCTACAAGTG

refseq_GIT2.F1 (forward primer)
refseq_GIT2.R1 (reverse primer)
Single exon skipping

Frame 0

EAPPPPPPLLSPGL*FPAGGRWEPCRNGSGAAR CALTAAGRILPGHQ*IGERFYVMSAAVSIGV*GA ISPK*GI*NTHRGLQHCFRWLRPCIITVLTLYG SILCWTLRLL*VEDVKLIHRIKYIPIKRNSSEP SIRC*RSSIACPAGMTIV*LPKILASNSIRA*EQ GILKPV*DCYL*EHKPTSFILKKETPHSMLPPK QGRFYRLNYWQYMEQTQAHRILVGKLPLIMQGK EGTMSWQSASWKYSMS*RTD*PSISVAGNQITKM DSTL*YLKWQTAAWICLNWQKLLRRNFNL*VII CLKNLPWMCTMKLTGERRMQSGLPRKTTAPW*P RQRSSPFFRSILSTHQHEIRADRS*LGSTPMSLP RWSLTFSVTPRGDSRAVLSRVQKTMWSSY*KPS ITSTALRVKTTISPTMTAWHQTKTQIWKPLQAK QTGRSFKHSRVKIRTSGNRPQPMYIRCKLVLSTQ TLPTTLP*RDVRLPGAVGPCPCTRPDQVRNHIS QWEKRAAPKRAG*DSSPSPRTHPGWRSRTAHLR VTTTTLPTTWSQMAWGQAERDGKEVWCGQGMAWY QTQQNPMWPQAPLSLAPKMSSGRLNRSPKTYRS S*EQPKKINMTVIFPAQRGYT*LLQKWQHYSPK NPSLIW*GLPFVY*RPVPTDCSQSARRPSQGTPA HPQTFSWSRSRSSSVRTTSPRLPSSWLPSPPKR TTTDKGRAPPPLFSRLYKVQFQIQTQNSSDFYK KKHLMTV*NFLKENSVLFLHVF*PIFNYLTHLPY FVPICWFSF*CSVVLSTTVELITYIQKYSFIV* SL*KLFHPLKLPAYG*NYK*NFS*NIFLREFS* KRKNQRHLFPAKHRAISEFPGLLHNL*VWGISKH VCRERTLPLFKTSVTVSLGRKQ*IGSFLLSVLP CYMFCYLMLLSQAPIS*CFLVILKAPWNEIKH* PTTGHLMKPCSG*ELHAHHHPLSGALPHPCKPDS LFKTCSKEKHQSNKPELILCHPFRYHYHEL*GK EITYSLTLCCTLHPKVSLLL*PLTAFSHELLHI FHHL*GLLIRKDVSYRSHLS*FLLHHLMST*KLD INFQEFLRECALAGCLLPQSSERVRLKCWNMLR LFFSYSKAPSCSECESSSRSCLLQPFCVCGSDP GLGPVMWMSEGSQVHGSRAEETMGSPPPTQ*GQ* PPAPRLLPCGKVGLGWPQL*FFKRQET*MFFGA ISPFINVGNQFEHFQRPCAGELCPLGCQLTAPT PGPCKLCSVRAWLPRTHLEQPLSLTRNGAGQPCV LCCCRSCF*PYVLSHSSHSHM*RRHKNQSTSCL NTC**LSFYFHRTDRCTPL*LDHMLS*K*HPDG ACFTKSPCVDAFVAFCFTVSSMGKGSTSPKDSST CLSHSPKCHIAVGCSRDAF*KGASHLKFRRHLI AFSAGFMSLADPPLSCKPLPLLSQGGRASVEAS GK*AVCRR*SSVFLGMFEDLQ*KMLPLWP*RKSR NMGTNKAFPVVRICQKSARLAHGAKTPNKFPGG MTWPLEGCRSSSLYVPRAFPWPHLRSGVRVLCS CWQIAPYLNICSSHLLHMDSVPCTSNVYLQ*RRS FSPCILNDPFLPICHPSPKADRAEVHFMKLALD *TEGNSCTSKLLSQKSSSALECK*FLFAKEQ*I GPKDQTQHFM*SEF*THNSGAVHGFSSDSVAEPR AKLACFCPQWHP*AFQ*YSFLSFLVLVEGNVFL EKKMLHRKTEMPFIPTCLIPVTHYSVLFIVHSI PVIIVPFKCCKIVLEFVPASYAINRLYK

Frame 1

KRRRHRRLCCPPASSSPQVGGGSHVETAPEQRG VR*LQRAGSFLGISK*GNVFM**VLQCPSESRA PYLPSEASETHTVASNTASDG*DLV**RC*LYMG AFFAGPCVYYEWKT*S*STG*STSQ*SGIHQSQ VSDVSVRPSLALPG*R*CDCQRS*QATPFEREN RES*NLFETVIFRSTSQLLSS*KRKHPTPCCLQS RADFTG*IIGSIWSRPRHTGF*WENSR*LCKAR RAP*AGRAPRGNTV*ANGQTSLLSLWQETRSQK WTALYNTSNGRQQPGFV*IGKSC*EETSISK*SF V*RTCHGCVR*S*QARDGCSLACHAKPQRPGNR DNGRPLSSGQS*VLINTKSGQTEVSSVQRP*VC HAGH*HSQ*RQEETAGQFSLGFKRQCGAHTENHQ *PAQR*ESRQRSARL*QRGIRRRHRFGNHCKQN KPAEASNTPE*KFEPQETGHNQCISGANWF*VH RHFQPLFLKETSVCPGQ*AHVHVRDRIRSETISP NGRSEPPRREQDETPALPRARIQAGEAEQHT*E *LRQHSQRHGARWHGVKPKGTAKKYGVARGWLG TRHSRTPCGPKPHSP*HRRCHQED*TDHQKHTGA LKSSPRK*T*QLYSLLREDTRSCYRNGSIIPQK TQV*YGEDFPSFTDVQCLPTAVRVQEDPPRGPR LTHRRSAGHAAGHPVCVRHRQGCQAAGYHHHQRE QQLTRAGHRLLCFLGFIKSNFKFRRRTLQISTK RNI**RFKTF*KKTQYYFCMFSNQFSTI*PTCL ILCLFAGLVSDVRLCCQQLLS*SHTSKSIVSLSK VCENFSIP*NSLPMAETINEIFHKTFS*ESSVK KEKISVICSLPSIVQ*VNFLAYFIISKFGVSPS MYVEKGLCRFLKLVSQSLLGGNNELVHFFSVFYP VTCSVI*CCYLKLLSLDVFLSS*RLHGMR*SID PPQDIS*SHVQGRSSTHITTHCQVLSLTPVSQT VCSKPAAKRSISLTSQNSFCATHLGITTMNYREK KSPTP*HCVVPFIQKSACFSDHLQLSHMSCFTF FIIFKDFL*GKMYRIAVI*VSFSSITS*VLKN* TSTFKSSLESVL*LAACYPKALKE*G*SAGTCSD CFLVTQKPLLVLNVKALPVPAYCSHSVCVVQTL A*GR*CG*VKGARYTAAGQRRLWGAHRLHSRGS SPQLHACCHVEKWA*GGHSFDFSRDKKPRCFLVP SLHL*MLATNLNTFRDHVQVSCVL*AASSQPPP QGPVSCAV*GPGFPGHTWSSL*VLPVMEPANPV CYVAVAPVFNHMFCPIAVIHTCEEDTRIKVQVAS IHANN*ASIFTGLIDAHLYSLTICCHESSTPTV LALLSHRVWMLLSHFASL*VAWEKGPRLQKTLP LVCPTALSATLLLDAPGMPSRRGPPTLSSGAI** PFLQASCP*LTLLYLANPCPCCPREDGLL*RLL GNELSAGGEAVFSLECLRTCNRRCCLYGPRESQ GTWGPTKHFQL*EYVRRVLG*PTVPKLQTSSPVE *RGHWRAAGRPLCMCPAHFLGLI*DLVSEFSAP VGKSHLI*TFVPATFCTWIPYLALAMSTFSNVV LFHPVFSMIPSSPYATLPPKLIGQRCTL*NWHLT EQKETAVPLSSFHRNRHLPWNANSSYLPKNSEL GPKIKHSISCNQNSKRTTRELCTASQVIQLQNQ EQNSPVFVLNGTHELSNSIVFFLSLSWLKVTCS* KRKCCIGKLRCLLFQRV*SLSLIILYFLLYILF L*LLCPLSVVKSFWNLCLLVMQ*TGSTS

Frame 2

SAAATAAFAVPRPLVPRRWEVGAMSKRLRSSEV CADCSGPDPSWASVNRGTFLCDECCSVHRSLGR HISQVRHLKHTPWPPTLLQMVETLYNNGANSIW EHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAK YQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRT GNLETCLRLLSLGAQANFFHPEKGNTPLHVASK AGQILQAELLAVYGADPGTQDSSGKTPVDYARQG GHHELAERLVEIQYELTDRLAFYLCGRKPDHKN GQHFIIPQMADSSLDLSELAKAAKKKLQSLSNH LFEELAMDVYDEVDRRETDAVWLATQNHSALVTE TTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFA TLVIDILSDAKRRQQGSSLSGSKDNVELILKTI NNQHSVESQDNDQPDYDSVASDEDTDLETTASKT NRQKLQTLQSENSNLRKQATTNVYQVQTGSEYT DTSNHSSLKRRPSARGSRPMSMYETGSGQKPYL PMGEASRPEESRMRLQPFPAHASRLEKQNSTPES DYDNTPNDMEPDGMGSSRKGRQRSMVWPGDGLV PDTAEPHVAPSPTLPSTEDVIRKTEQITKNIQE LLRAAQENKHDSYIPCSERIHVAVTEMAALFPKK PKSDMVRTSLRLLTSSAYRLQSECKKTLPGDPG SPTDVQLVTQQVIQCAYDIAKAAKQLVTITTKE NNN*QGQGTASSVF*AL*SPISNSDAELFRFLQK ETFNDGLKLFKRKLSIIFACFLTNFQLFNPLAL FCAYLLV*FLMFGCVVNNC*VDHIHPKV*FHCL KFVKTFPSLKTPCLWLKL*MKFFIKHFLERVQLK KKKSASFVPCQASCNK*ISWPTS*SLSLGYLQA CM*RKDFAAF*N*CHSLSWEETMNWFISSQCFT LLHVLLFNVVISSSYLLMFSCHLEGSME*DKALT HHRTSHEAMFRVGAPRTSPPIVRCSPSPL*ARQ FVQNLQQREASV*QARTHFVPPI*VSLP*TIGK RNHLLPDTVLYPSSKSQLASLTTYSFLT*VASHF SSSLRTSYKERCIVSQSSELVSPPSPHEYLKIR HQLSRVP*RVCSSWLPATPKL*KSKVEVLEHAQ IVF*LLKSPFLF*M*KLFPFLLTAAILCVWFRPW PRAGDVDE*REPGTRQQGRGDYGEPTAYTVGAV APSSTPVAMWKSGPRVATALIFQETRNLDVFWC HLSIYKCWQPI*TLSETMCR*AVSSRLPAHSPHP RAL*AVQCEGLASPDTPGAASESYP*WSRPTLC VMLLSLLFLTICSVP*QSFTHVKKTQESKYKLP QYMLITKLLFSQD*SMHTFIA*PYAVMKVAPRRC LLY*VTVCGCFCRILLHCE*HGKRVHVSKRLFH LFVPQP*VPHCCWMLPGCLLEGGLPP*VQAPSD SLFCRLHVLS*PSFILQTPAPAVPGRTGFCRGFW EMSCLQEVKQCFPWNV*GPAIEDVAFMALEKVK EHGDQQSISSCKNMSEECSVSPRCQNSKQVPRW NDVAIGGLQVVLSVCAPRISLASSEIWCQSSLLL LANRTLSEHLFQPPFAHGFRTLH*QCLPSVTSF FFTLYSQ*SLPPHMPPFPQS**GRGALYETGT* LNRRKQLYL*APFTEIVICLGMQIVLICQRTVNW AQRSNTAFHVIRILNAQLGSCARLLK*FSCRTK SKTRLFLSSMAPMSFPIV*FSFFPCPG*R*RVP RKENAA*EN*DAFYSNVFDPCHSLFCTFYCTFYS CNYCAL*VL*NRFGICAC*LCNKQALQV

Sequence NM_057169

GAAGCGCCGCCGCCACCGCCGCCTTTGCTGTCCCCCGGCCTCTAGTTCCCCGCAGGTGGGAGGTGGGAGCCATGTCGAAACGGCTCCGGAGCAGCGAGGT GTGCGCTGACTGCAGCGGGCCGGATCCTTCCTGGGCATCAGTAAATAGGGGAACGTTTTTATGTGATGAGTGCTGCAGTGTCCATCGGAGTCTAGGGCGC CATATCTCCCAAGTGAGGCATCTGAAACACACACCGTGGCCTCCAACACTGCTTCAGATGGTTGAGACCTTGTATAATAACGGTGCTAACTCTATATGGG AGCATTCTTTGCTGGACCCTGCGTCTATTATGAGTGGAAGACGTAAAGCTAATCCACAGGATAAAGTACATCCCAATAAAGCGGAATTCATCAGAGCCAA GTATCAGATGTTAGCGTTCGTCCATCGCTTGCCCTGCCGGGATGACGATAGTGTGACTGCCAAAGATCTTAGCAAGCAACTCCATTCGAGCGTGAGAACA GGGAATCTTGAAACCTGTTTGAGACTGTTATCTTTAGGAGCACAAGCCAACTTCTTTCATCCTGAAAAAGGAAACACCCCACTCCATGTTGCCTCCAAAG CAGGGCAGATTTTACAGGCTGAATTATTGGCAGTATATGGAGCAGACCCAGGCACACAGGATTCTAGTGGGAAAACTCCCGTTGATTATGCAAGGCAAGG AGGGCACCATGAGCTGGCAGAGCGCCTCGTGGAAATACAGTATGAGCTAACGGACAGACTAGCCTTCTATCTCTGTGGCAGGAAACCAGATCACAAAAAT GGACAGCACTTTATAATACCTCAAATGGCAGACAGCAGCCTGGATTTGTCTGAATTGGCAAAAGCTGCTAAGAAGAAACTTCAATCTCTAAGTAATCATT TGTTTGAAGAACTTGCCATGGATGTGTACGATGAAGTTGACAGGCGAGAGACGGATGCAGTCTGGCTTGCCACGCAAAACCACAGCGCCCTGGTAACCGA GACAACGGTCGTCCCCTTTCTTCCGGTCAATCCTGAGTACTCATCAACACGAAATCAGGGCAGACAGAAGTTAGCTCGGTTCAACGCCCATGAGTTTGCC ACGCTGGTCATTGACATTCTCAGTGACGCCAAGAGGAGACAGCAGGGCAGTTCTCTCTCGGGTTCAAAAGACAATGTGGAGCTCATACTGAAAACCATCA ATAACCAGCACAGCGTTGAGAGTCAAGACAACGATCAGCCCGACTATGACAGCGTGGCATCAGACGAAGACACAGATTTGGAAACCACTGCAAGCAAAAC AAACCGGCAGAAGAGCCTAGATTCAGATTTATCAGATGGACCAGTCACTGTACAGGAATTTATGGAGGTCAAAAACGCTCTAGTGGCTTCTGAGGCCAAG ATACAGCAGCTAATGAAGGTGAATAACAACTTGAGTGACGAGCTGAGAATTATGCAGAAAAAGCTTCAAACACTCCAGAGTGAAAATTCGAACCTCAGGA AACAGGCCACAACCAATGTATATCAGGTGCAAACTGGTTCTGAGTACACAGACACTTCCAACCACTCTTCCTTAAAGAGACGTCCGTCTGCCCGGGGCAG TAGGCCCATGTCCATGTACGAGACCGGATCAGGTCAGAAACCATATCTCCCAATGGGAGAAGCGAGCCGCCCCGAAGAGAGCAGGATGAGACTCCAGCCC TTCCCCGCGCACATCGGGAGGAGTGCACTTGTGACCTCCTCTTCATCTCTGCCTTCCTTCCCCTCCACACTTTCCTGGTCGAGGGACGAAAGCGCCCGAA GGGCATCCAGGCTGGAGAAGCAGAACAGCACACCTGAGAGTGACTACGACAACACTCCCAACGACATGGAGCCAGATGGCATGGGGTCAAGCCGAAAGGG ACGGCAAAGAAGTATGGTGTGGCCAGGGGATGGCTTGGTACCAGACACAGCAGAACCCCATGTGGCCCCAAGCCCCACTCTCCCTAGCACCGAAGATGTC ATCAGGAAGACTGAACAGATCACCAAAAACATACAGGAGCTCTTAAGAGCAGCCCAAGAAAATAAACATGACAGTTATATTCCCTGCTCAGAGAGGATAC ACGTAGCTGTTACAGAAATGGCAGCATTATTCCCCAAAAAACCCAAGTCTGATATGGTGAGGACTTCCCTTCGTTTACTGACGTCCAGTGCCTACCGACT GCAGTCAGAGTGCAAGAAGACCCTCCCAGGGGACCCCGGCTCACCCACAGACGTTCAGCTGGTCACGCAGCAGGTCATCCAGTGTGCGTACGACATCGCC AAGGCTGCCAAGCAGCTGGTTACCATCACCACCAAAGAGAACAACAACTGACAAGGGCAGGGCACCGCCTCCTCTGTTTTCTAGGCTTTATAAAGTCCAA TTTCAAATTCAGACGCAGAACTCTTCAGATTTCTACAAAAAGAAACATTTAATGACGGTTTAAAACTTTTTAAAAGAAAACTCAGTATTATTTTTGCATG TTTTCTAACCAATTTTCAACTATTTAACCCACTTGCCTTATTTTGTGCCTATTTGCTGGTTTAGTTTCTGATGTTCGGTTGTGTTGTCAACAACTGTTGA GTTGATCACATACATCCAAAAGTATAGTTTCATTGTCTAAAGTTTGTGAAAACTTTTCCATCCCTTAAAACTCCCTGCCTATGGCTGAAACTATAAATGA AATTTTTCATAAAACATTTTCTTGAGAGAGTTCAGTTAAAAAAGAAAAAATCAGCGTCATTTGTTCCCTGCCAAGCATCGTGCAATAAGTGAATTTCCTG GCCTACTTCATAATCTCTAAGTTTGGGGTATCTCCAAGCATGTATGTAGAGAAAGGACTTTGCCGCTTTTTAAAACTAGTGTCACAGTCTCTCTTGGGAG GAAACAATGAATTGGTTCATTTCTTCTCAGTGTTTTACCCTGTTACATGTTCTGTTATTTAATGTTGTTATCTCAAGCTCCTATCTCTTGATGTTTTCTT GTCATCTTGAAGGCTCCATGGAATGAGATAAAGCATTGACCCACCACAGGACATCTCATGAAGCCATGTTCAGGGTAGGAGCTCCACGCACATCACCACC CATTGTCAGGTGCTCTCCCTCACCCCTGTAAGCCAGACAGTTTGTTCAAAACCTGCAGCAAAGAGAAGCATCAGTCTAACAAGCCAGAACTCATTTTGTG CCACCCATTTAGGTATCACTACCATGAACTATAGGGAAAAGAAATCACCTACTCCCTGACACTGTGTTGTACCCTTCATCCAAAAGTCAGCTTGCTTCTC TGACCACTTACAGCTTTCTCACATGAGTTGCTTCACATTTTTCATCATCTTTAAGGACTTCTTATAAGGAAAGATGTATCGTATCGCAGTCATCTGAGTT AGTTTCTCCTCCATCACCTCATGAGTACTTAAAAATTAGACATCAACTTTCAAGAGTTCCTTAGAGAGTGTGCTCTAGCTGGCTGCCTGCTACCCCAAAG CTCTGAAAGAGTAAGGTTGAAGTGCTGGAACATGCTCAGATTGTTTTTTAGTTACTCAAAAGCCCCTTCTTGTTCTGAATGTGAAAGCTCTTCCCGTTCC TGCTTACTGCAGCCATTCTGTGTGTGTGGTTCAGACCCTGGCCTAGGGCCGGTGATGTGGATGAGTGAAGGGAGCCAGGTACACGGCAGCAGGGCAGAGG AGACTATGGGGAGCCCACCGCCTACACAGTAGGGGCAGTAGCCCCCAGCTCCACGCCTGTTGCCATGTGGAAAAGTGGGCCTAGGGTGGCCACAGCTTTG ATTTTTCAAGAGACAAGAAACCTAGATGTTTTTTGGTGCCATCTCTCCATTTATAAATGTTGGCAACCAATTTGAACACTTTCAGAGACCATGTGCAGGT GAGCTGTGTCCTCTAGGCTGCCAGCTCACAGCCCCCACCCCAGGGCCCTGTAAGCTGTGCAGTGTGAGGGCCTGGCTTCCCCGGACACACCTGGAGCAGC CTCTGAGTCTTACCCGTAATGGAGCCGGCCAACCCTGTGTGTTATGTTGCTGTCGCTCCTGTTTTTAACCATATGTTCTGTCCCATAGCAGTCATTCACA CATGTGAAGAAGACACAAGAATCAAAGTACAAGTTGCCTCAATACATGCTAATAACTAAGCTTCTATTTTCACAGGACTGATCGATGCACACCTTTATAG CTTGACCATATGCTGTCATGAAAGTAGCACCCCGACGGTGCTTGCTTTACTAAGTCACCGTGTGTGGATGCTTTTGTCGCATTTTGCTTCACTGTGAGTA GCATGGGAAAAGGGTCCACGTCTCCAAAAGACTCTTCCACTTGTTTGTCCCACAGCCCTAAGTGCCACATTGCTGTTGGATGCTCCCGGGATGCCTTCTA GAAGGGGGCCTCCCACCTTAAGTTCAGGCGCCATCTGATAGCCTTTTCTGCAGGCTTCATGTCCTTAGCTGACCCTCCTTTATCTTGCAAACCCCTGCCC CTGCTGTCCCAGGGAGGACGGGCTTCTGTAGAGGCTTCTGGGAAATGAGCTGTCTGCAGGAGGTGAAGCAGTGTTTTCCTTGGAATGTTTGAGGACCTGC AATAGAAGATGTTGCCTTTATGGCCCTAGAGAAAGTCAAGGAACATGGGGACCAACAAAGCATTTCCAGTTGTAAGAATATGTCAGAAGAGTGCTCGGTT AGCCCACGGTGCCAAAACTCCAAACAAGTTCCCCGGTGGAATGACGTGGCCATTGGAGGGCTGCAGGTCGTCCTCTCTGTATGTGCCCCGCGCATTTCCT TGGCCTCATCTGAGATCTGGTGTCAGAGTTCTCTGCTCCTGTTGGCAAATCGCACCTTATCTGAACATTTGTTCCAGCCACCTTTTGCACATGGATTCCG TACCTTGCACTAGCAATGTCTACCTTCAGTAACGTCGTTCTTTTTCACCCTGTATTCTCAATGATCCCTTCCTCCCCATATGCCACCCTTCCCCCAAAGC TGATAGGGCAGAGGTGCACTTTATGAAACTGGCACTTGACTGAACAGAAGGAAACAGCTGTACCTCTAAGCTCCTTTCACAGAAATCGTCATCTGCCTTG GAATGCAAATAGTTCTTATTTGCCAAAGAACAGTGAATTGGGCCCAAAGATCAAACACAGCATTTCATGTAATCAGAATTCTAAACGCACAACTCGGGAG CTGTGCACGGCTTCTCAAGTGATTCAGTTGCAGAACCAAGAGCAAAACTCGCCTGTTTTTGTCCTCAATGGCACCCATGAGCTTTCCAATAGTATAGTTT TCTTTCTTTCCTTGTCCTGGTTGAAGGTAACGTGTTCCTAGAAAAGAAAATGCTGCATAGGAAAACTGAGATGCCTTTTATTCCAACGTGTTTGATCCCT GTCACTCATTATTCTGTACTTTTTATTGTACATTCTATTCCTGTAATTATTGTGCCCTTTAAGTGTTGTAAAATCGTTTTGGAATTTGTGCCTGCTAGTT ATGCAATAAACAGGCTCTACAAGTG

refseq_GIT2.F1 (forward primer)
refseq_GIT2.R1 (reverse primer)
Single exon skipping

Frame 0

EAPPPPPPLLSPGL*FPAGGRWEPCRNGSGAAR CALTAAGRILPGHQ*IGERFYVMSAAVSIGV*GA ISPK*GI*NTHRGLQHCFRWLRPCIITVLTLYG SILCWTLRLL*VEDVKLIHRIKYIPIKRNSSEP SIRC*RSSIACPAGMTIV*LPKILASNSIRA*EQ GILKPV*DCYL*EHKPTSFILKKETPHSMLPPK QGRFYRLNYWQYMEQTQAHRILVGKLPLIMQGK EGTMSWQSASWKYSMS*RTD*PSISVAGNQITKM DSTL*YLKWQTAAWICLNWQKLLRRNFNL*VII CLKNLPWMCTMKLTGERRMQSGLPRKTTAPW*P RQRSSPFFRSILSTHQHEIRADRS*LGSTPMSLP RWSLTFSVTPRGDSRAVLSRVQKTMWSSY*KPS ITSTALRVKTTISPTMTAWHQTKTQIWKPLQAK QTGRRA*IQIYQMDQSLYRNLWRSKTL*WLLRPR YSS**R*ITT*VTS*ELCRKSFKHSRVKIRTSG NRPQPMYIRCKLVLSTQTLPTTLP*RDVRLPGA VGPCPCTRPDQVRNHISQWEKRAAPKRAG*DSSP SPRTSGGVHL*PPLHLCLPSPPHFPGRGTKAPE GHPGWRSRTAHLRVTTTTLPTTWSQMAWGQAER DGKEVWCGQGMAWYQTQQNPMWPQAPLSLAPKMS SGRLNRSPKTYRSS*EQPKKINMTVIFPAQRGY T*LLQKWQHYSPKNPSLIW*GLPFVY*RPVPTD CSQSARRPSQGTPAHPQTFSWSRSRSSSVRTTSP RLPSSWLPSPPKRTTTDKGRAPPPLFSRLYKVQ FQIQTQNSSDFYKKKHLMTV*NFLKENSVLFLH VF*PIFNYLTHLPYFVPICWFSF*CSVVLSTTVE LITYIQKYSFIV*SL*KLFHPLKLPAYG*NYK* NFS*NIFLREFS*KRKNQRHLFPAKHRAISEFP GLLHNL*VWGISKHVCRERTLPLFKTSVTVSLGR KQ*IGSFLLSVLPCYMFCYLMLLSQAPIS*CFL VILKAPWNEIKH*PTTGHLMKPCSG*ELHAHHH PLSGALPHPCKPDSLFKTCSKEKHQSNKPELILC HPFRYHYHEL*GKEITYSLTLCCTLHPKVSLLL *PLTAFSHELLHIFHHL*GLLIRKDVSYRSHLS *FLLHHLMST*KLDINFQEFLRECALAGCLLPQS SERVRLKCWNMLRLFFSYSKAPSCSECESSSRS CLLQPFCVCGSDPGLGPVMWMSEGSQVHGSRAE ETMGSPPPTQ*GQ*PPAPRLLPCGKVGLGWPQL* FFKRQET*MFFGAISPFINVGNQFEHFQRPCAG ELCPLGCQLTAPTPGPCKLCSVRAWLPRTHLEQ PLSLTRNGAGQPCVLCCCRSCF*PYVLSHSSHSH M*RRHKNQSTSCLNTC**LSFYFHRTDRCTPL* LDHMLS*K*HPDGACFTKSPCVDAFVAFCFTVS SMGKGSTSPKDSSTCLSHSPKCHIAVGCSRDAF* KGASHLKFRRHLIAFSAGFMSLADPPLSCKPLP LLSQGGRASVEASGK*AVCRR*SSVFLGMFEDL Q*KMLPLWP*RKSRNMGTNKAFPVVRICQKSARL AHGAKTPNKFPGGMTWPLEGCRSSSLYVPRAFP WPHLRSGVRVLCSCWQIAPYLNICSSHLLHMDS VPCTSNVYLQ*RRSFSPCILNDPFLPICHPSPKA DRAEVHFMKLALD*TEGNSCTSKLLSQKSSSAL ECK*FLFAKEQ*IGPKDQTQHFM*SEF*THNSG AVHGFSSDSVAEPRAKLACFCPQWHP*AFQ*YSF LSFLVLVEGNVFLEKKMLHRKTEMPFIPTCLIP VTHYSVLFIVHSIPVIIVPFKCCKIVLEFVPAS YAINRLYK

Frame 1

KRRRHRRLCCPPASSSPQVGGGSHVETAPEQRG VR*LQRAGSFLGISK*GNVFM**VLQCPSESRA PYLPSEASETHTVASNTASDG*DLV**RC*LYMG AFFAGPCVYYEWKT*S*STG*STSQ*SGIHQSQ VSDVSVRPSLALPG*R*CDCQRS*QATPFEREN RES*NLFETVIFRSTSQLLSS*KRKHPTPCCLQS RADFTG*IIGSIWSRPRHTGF*WENSR*LCKAR RAP*AGRAPRGNTV*ANGQTSLLSLWQETRSQK WTALYNTSNGRQQPGFV*IGKSC*EETSISK*SF V*RTCHGCVR*S*QARDGCSLACHAKPQRPGNR DNGRPLSSGQS*VLINTKSGQTEVSSVQRP*VC HAGH*HSQ*RQEETAGQFSLGFKRQCGAHTENHQ *PAQR*ESRQRSARL*QRGIRRRHRFGNHCKQN KPAEEPRFRFIRWTSHCTGIYGGQKRSSGF*GQ DTAANEGE*QLE*RAENYAEKASNTPE*KFEPQE TGHNQCISGANWF*VHRHFQPLFLKETSVCPGQ *AHVHVRDRIRSETISPNGRSEPPRREQDETPA LPRAHREECTCDLLFISAFLPLHTFLVEGRKRPK GIQAGEAEQHT*E*LRQHSQRHGARWHGVKPKG TAKKYGVARGWLGTRHSRTPCGPKPHSP*HRRC HQED*TDHQKHTGALKSSPRK*T*QLYSLLREDT RSCYRNGSIIPQKTQV*YGEDFPSFTDVQCLPT AVRVQEDPPRGPRLTHRRSAGHAAGHPVCVRHR QGCQAAGYHHHQREQQLTRAGHRLLCFLGFIKSN FKFRRRTLQISTKRNI**RFKTF*KKTQYYFCM FSNQFSTI*PTCLILCLFAGLVSDVRLCCQQLL S*SHTSKSIVSLSKVCENFSIP*NSLPMAETINE IFHKTFS*ESSVKKEKISVICSLPSIVQ*VNFL AYFIISKFGVSPSMYVEKGLCRFLKLVSQSLLG GNNELVHFFSVFYPVTCSVI*CCYLKLLSLDVFL SS*RLHGMR*SIDPPQDIS*SHVQGRSSTHITT HCQVLSLTPVSQTVCSKPAAKRSISLTSQNSFC ATHLGITTMNYREKKSPTP*HCVVPFIQKSACFS DHLQLSHMSCFTFFIIFKDFL*GKMYRIAVI*V SFSSITS*VLKN*TSTFKSSLESVL*LAACYPK ALKE*G*SAGTCSDCFLVTQKPLLVLNVKALPVP AYCSHSVCVVQTLA*GR*CG*VKGARYTAAGQR RLWGAHRLHSRGSSPQLHACCHVEKWA*GGHSF DFSRDKKPRCFLVPSLHL*MLATNLNTFRDHVQV SCVL*AASSQPPPQGPVSCAV*GPGFPGHTWSS L*VLPVMEPANPVCYVAVAPVFNHMFCPIAVIH TCEEDTRIKVQVASIHANN*ASIFTGLIDAHLYS LTICCHESSTPTVLALLSHRVWMLLSHFASL*V AWEKGPRLQKTLPLVCPTALSATLLLDAPGMPS RRGPPTLSSGAI**PFLQASCP*LTLLYLANPCP CCPREDGLL*RLLGNELSAGGEAVFSLECLRTC NRRCCLYGPRESQGTWGPTKHFQL*EYVRRVLG *PTVPKLQTSSPVE*RGHWRAAGRPLCMCPAHFL GLI*DLVSEFSAPVGKSHLI*TFVPATFCTWIP YLALAMSTFSNVVLFHPVFSMIPSSPYATLPPK LIGQRCTL*NWHLTEQKETAVPLSSFHRNRHLPW NANSSYLPKNSELGPKIKHSISCNQNSKRTTRE LCTASQVIQLQNQEQNSPVFVLNGTHELSNSIV FFLSLSWLKVTCS*KRKCCIGKLRCLLFQRV*SL SLIILYFLLYILFL*LLCPLSVVKSFWNLCLLV MQ*TGSTS

Frame 2

SAAATAAFAVPRPLVPRRWEVGAMSKRLRSSEV CADCSGPDPSWASVNRGTFLCDECCSVHRSLGR HISQVRHLKHTPWPPTLLQMVETLYNNGANSIW EHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAK YQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRT GNLETCLRLLSLGAQANFFHPEKGNTPLHVASK AGQILQAELLAVYGADPGTQDSSGKTPVDYARQG GHHELAERLVEIQYELTDRLAFYLCGRKPDHKN GQHFIIPQMADSSLDLSELAKAAKKKLQSLSNH LFEELAMDVYDEVDRRETDAVWLATQNHSALVTE TTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFA TLVIDILSDAKRRQQGSSLSGSKDNVELILKTI NNQHSVESQDNDQPDYDSVASDEDTDLETTASKT NRQKSLDSDLSDGPVTVQEFMEVKNALVASEAK IQQLMKVNNNLSDELRIMQKKLQTLQSENSNLR KQATTNVYQVQTGSEYTDTSNHSSLKRRPSARGS RPMSMYETGSGQKPYLPMGEASRPEESRMRLQP FPAHIGRSALVTSSSSLPSFPSTLSWSRDESAR RASRLEKQNSTPESDYDNTPNDMEPDGMGSSRKG RQRSMVWPGDGLVPDTAEPHVAPSPTLPSTEDV IRKTEQITKNIQELLRAAQENKHDSYIPCSERI HVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRL QSECKKTLPGDPGSPTDVQLVTQQVIQCAYDIA KAAKQLVTITTKENNN*QGQGTASSVF*AL*SP ISNSDAELFRFLQKETFNDGLKLFKRKLSIIFAC FLTNFQLFNPLALFCAYLLV*FLMFGCVVNNC* VDHIHPKV*FHCLKFVKTFPSLKTPCLWLKL*M KFFIKHFLERVQLKKKKSASFVPCQASCNK*ISW PTS*SLSLGYLQACM*RKDFAAF*N*CHSLSWE ETMNWFISSQCFTLLHVLLFNVVISSSYLLMFS CHLEGSME*DKALTHHRTSHEAMFRVGAPRTSPP IVRCSPSPL*ARQFVQNLQQREASV*QARTHFV PPI*VSLP*TIGKRNHLLPDTVLYPSSKSQLAS LTTYSFLT*VASHFSSSLRTSYKERCIVSQSSEL VSPPSPHEYLKIRHQLSRVP*RVCSSWLPATPK L*KSKVEVLEHAQIVF*LLKSPFLF*M*KLFPF LLTAAILCVWFRPWPRAGDVDE*REPGTRQQGRG DYGEPTAYTVGAVAPSSTPVAMWKSGPRVATAL IFQETRNLDVFWCHLSIYKCWQPI*TLSETMCR *AVSSRLPAHSPHPRAL*AVQCEGLASPDTPGAA SESYP*WSRPTLCVMLLSLLFLTICSVP*QSFT HVKKTQESKYKLPQYMLITKLLFSQD*SMHTFI A*PYAVMKVAPRRCLLY*VTVCGCFCRILLHCE* HGKRVHVSKRLFHLFVPQP*VPHCCWMLPGCLL EGGLPP*VQAPSDSLFCRLHVLS*PSFILQTPA PAVPGRTGFCRGFWEMSCLQEVKQCFPWNV*GPA IEDVAFMALEKVKEHGDQQSISSCKNMSEECSV SPRCQNSKQVPRWNDVAIGGLQVVLSVCAPRIS LASSEIWCQSSLLLLANRTLSEHLFQPPFAHGFR TLH*QCLPSVTSFFFTLYSQ*SLPPHMPPFPQS **GRGALYETGT*LNRRKQLYL*APFTEIVICL GMQIVLICQRTVNWAQRSNTAFHVIRILNAQLGS CARLLK*FSCRTKSKTRLFLSSMAPMSFPIV*F SFFPCPG*R*RVPRKENAA*EN*DAFYSNVFDP CHSLFCTFYCTFYSCNYCAL*VL*NRFGICAC*L CNKQALQV