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Sequence NM_006870
GGGAAGCTCCCCTCCGAGACTCTGGATAGCCGCCGGGGGGGCGTTGCCTGGCCGGCTGGTTGCGTAGCAACGGCAGGCGGTGCGTGACGTGCGCAGCCGCGTTCCGTCCTGAGGCGCGCCCGCCCCGGGGTAAGCTCGCGCCGCCGCGTCAGCTCAGCGCTGGGTCTCTCGGTCCCGCAGCCGTGAGGAGGACGGTCTGC
ATACTCGCTGCCCGCCGGCTCCCTCCCCCGCGTCCCTGCGACCGCCGCGGCGAAGATGGCCTCAGGAGTGCAAGTAGCTGATGAAGTATGTCGCATTTTT
TATGACATGAAAGTTCGTAAATGCTCCACACCAGAAGAAATCAAGAAAAGAAAGAAGGCTGTCATTTTTTGTCTCAGTGCAGACAAAAAGTGCATCATTG
TAGAAGAAGGCAAAGAGATCTTGGTTGGAGATGTTGGTGTAACCATAACTGATCCTTTCAAGCATTTTGTGGGAATGCTTCCTGAAAAAGATTGTCGCTA
TGCTTTGTATGATGCAAGCTTTGAAACAAAAGAATCCAGAAAAGAAGAGTTGATGTTTTTTTTGTGGGCACCAGAACTAGCACCTCTGAAAAGTAAAATG
ATCTATGCAAGCTCCAAGGATGCAATTAAAAAGAAATTTCAAGGCATAAAACATGAATGTCAAGCAAATGGACCAGAAGATCTCAATCGGGCTTGTATTG
CTGAAAAGTTAGGTGGATCCTTAATTGTAGCCTTTGAAGGATGCCCTGTGTAGATTATTCAGTGCCACAAATTGAAAGCTTCCATGTTTAATGTTATCCT
CTTGCTATATAAATAAAGCAAATATATTTAGGCCAGGGTCTCACTGAGGGGGAGCTGTCTTGTCATCTTTTAGAGTAAACTATTCTATAAACATATGCAA
ACAGCCCTAAATAAATCTAAAGTCTAAAGTTTTATTGATGTGAAATTAAATTCTTATTGGCCAAATGCCTGTTTTGATGAGTTGATTTATAAAGATTTTT
GTTAAGCTCAGGATTTTAAATTACACAGTTCACAAACAGTAAAGGCCATGTGAAGAGAATTATTACATCTTTATTAACCTCAGCATTTACTTTGTTTCTT
TTGCTTAGGAAATTGCTCATAATCTGGTTATAATTTTGGTCCAAATTCTTTATTCTTCCTTGAGCTAAGCAGAATAATGGAATATAATATGTCTTCATAA
TATAACAACACTAATACACTAATAGTAAGATTAAGTTAGGCAGTCTTCTACCAAATGTGTAATGGAGATTGCCTCAAAATTGTGTCCACATAATCCACGC
TCATCTTGCAAAGCGCTATTTCAGGCACATCATTGGAATACAGGAAGTAGCCCTGCACCTGCCAGTGAGCTCGCCATTCACTGATTGGAAGAGTGACCTG
GCATCTTGGAAATCATTGTGTGTCTTCAGGAGAATGTGCAGTGTCTTGTAACAACTAATTATAATGCAAATTAGGGCTACATTGTAATCTGCTTTGTTAA
TGAAAATGATAAAACAGAATATTGACAAGCTAGGACACCTGTGGTATCTTTAATTGTATCTCCTTCAGAAGTTTGCTTCTTATGGTATAATAAAGTATGG
AAGAATATTGAGTA
- refseq_DSTN.F1 (forward primer)
- refseq_DSTN.R1 (reverse primer)
- Single exon skipping
Frame 0
GKLPSETLDSRRGGVAWPAGCVATAGGA*RAQPRSVLRRARPGVSSRRRVSSALGLSVPQP*GGRSA
YSLPAGSLPRVPATAAAKMASGVQVADEVCRIF
YDMKVRKCSTPEEIKKRKKAVIFCLSADKKCII
VEEGKEILVGDVGVTITDPFKHFVGMLPEKDCRY
ALYDASFETKESRKEELMFFLWAPELAPLKSKM
IYASSKDAIKKKFQGIKHECQANGPEDLNRACI
AEKLGGSLIVAFEGCPV*IIQCHKLKASMFNVIL
LLYK*SKYI*ARVSLRGSCLVIF*SKLFYKHMQ
TALNKSKV*SFIDVKLNSYWPNACFDELIYKDF
C*AQDFKLHSSQTVKAM*RELLHLY*PQHLLCFF
CLGNCS*SGYNFGPNSLFFLELSRIMEYNMSS*
YNNTNTLIVRLS*AVFYQMCNGDCLKIVST*ST
LILQSAISGTSLEYRK*PCTCQ*ARHSLIGRVTW
HLGNHCVSSGECAVSCNN*L*CKLGLHCNLLC*
*K**NRILTS*DTCGIFNCISFRSLLLMV**SM
EEY*V
Frame 1
GSSPPRLWIAAGGALPGRLVA*QRQAVRDVRSRVPS*GAPAPG*ARAAASAQRWVSRSRSREEDGL
HTRCPPAPSPASLRPPRRRWPQECK*LMKYVAFF
MT*KFVNAPHQKKSRKERRLSFFVSVQTKSASL
*KKAKRSWLEMLV*P*LILSSILWECFLKKIVA
MLCMMQALKQKNPEKKS*CFFCGHQN*HL*KVK*
SMQAPRMQLKRNFKA*NMNVKQMDQKISIGLVL
LKS*VDP*L*PLKDALCRLFSATN*KLPCLMLS
SCYINKANIFRPGSH*GGAVLSSFRVNYSINICK
QP*INLKSKVLLM*N*ILIGQMPVLMS*FIKIF
VKLRILNYTVHKQ*RPCEENYYIFINLSIYFVS
FA*EIAHNLVIILVQILYSSLS*AE*WNIICLHN
ITTLIH***D*VRQSSTKCVMEIASKLCPHNPR
SSCKALFQAHHWNTGSSPAPASELAIH*LEE*P
GILEIIVCLQENVQCLVTTNYNAN*GYIVICFVN
ENDKTEY*QARTPVVSLIVSPSEVCFLWYNKVW
KNIE
Frame 2
EAPLRDSG*PPGGRCLAGWLRSNGRRCVTCAAAFRPEARPPRGKLAPPRQLSAGSLGPAAVRRTVC
ILAARRLPPPRPCDRRGEDGLRSASS**SMSHF
L*HESS*MLHTRRNQEKKEGCHFLSQCRQKVHHC
RRRQRDLGWRCWCNHN*SFQAFCGNAS*KRLSL
CFV*CKL*NKRIQKRRVDVFFVGTRTSTSEK*N
DLCKLQGCN*KEISRHKT*MSSKWTRRSQSGLYC
*KVRWILNCSL*RMPCVDYSVPQIESFHV*CYP
LAI*IKQIYLGQGLTEGELSCHLLE*TIL*TYA
NSPK*I*SLKFY*CEIKFLLAKCLF**VDL*RFL
LSSGF*ITQFTNSKGHVKRIITSLLTSAFTLFL
LLRKLLIIWL*FWSKFFILP*AKQNNGI*YVFI
I*QH*YTNSKIKLGSLLPNV*WRLPQNCVHIIHA
HLAKRYFRHIIGIQEVALHLPVSSPFTDWKSDL
ASWKSLCVFRRMCSVL*QLIIMQIRATL*SALL
MKMIKQNIDKLGHLWYL*LYLLQKFASYGIIKYG
RILS
Sequence NM_001011546
GGGAAGCTCCCCTCCGAGACTCTGGATAGCCGCCGGGGGGGCGTTGCCTGGCCGGCTGGTTGCGTAGCAACGGCAGGCGGTGCGTGACGTGCGCAGCCGCGTTCCGTCCTGAGGCGCGCCCGCCCCGGGGTAAGCTCGCGCCGCCGCGTCAGCTCAGCGCTGGGTCTCTCGGTCCCGCAGCCGTGAGGAGGACGGTCTGC
ATACTCGCTGCCCGCCGGCTCCCTCCCCCGCGTCCCTGCGACCGCCGCGGCGAAGATGATTTTCAGTTCACCCACTAGAGGCAAAGACGTAAGAGAGCTA
CCAGCGTATTGAGAGGAGATACACAGGCACTACGAGAATGGAGTTTAGAAGAAGGTCAGAGCCCTGGGCAGTGTTGGGCCAGGCTTTAGGGCCTCAGGAG
TGCAAGTAGCTGATGAAGTATGTCGCATTTTTTATGACATGAAAGTTCGTAAATGCTCCACACCAGAAGAAATCAAGAAAAGAAAGAAGGCTGTCATTTT
TTGTCTCAGTGCAGACAAAAAGTGCATCATTGTAGAAGAAGGCAAAGAGATCTTGGTTGGAGATGTTGGTGTAACCATAACTGATCCTTTCAAGCATTTT
GTGGGAATGCTTCCTGAAAAAGATTGTCGCTATGCTTTGTATGATGCAAGCTTTGAAACAAAAGAATCCAGAAAAGAAGAGTTGATGTTTTTTTTGTGGG
CACCAGAACTAGCACCTCTGAAAAGTAAAATGATCTATGCAAGCTCCAAGGATGCAATTAAAAAGAAATTTCAAGGCATAAAACATGAATGTCAAGCAAA
TGGACCAGAAGATCTCAATCGGGCTTGTATTGCTGAAAAGTTAGGTGGATCCTTAATTGTAGCCTTTGAAGGATGCCCTGTGTAGATTATTCAGTGCCAC
AAATTGAAAGCTTCCATGTTTAATGTTATCCTCTTGCTATATAAATAAAGCAAATATATTTAGGCCAGGGTCTCACTGAGGGGGAGCTGTCTTGTCATCT
TTTAGAGTAAACTATTCTATAAACATATGCAAACAGCCCTAAATAAATCTAAAGTCTAAAGTTTTATTGATGTGAAATTAAATTCTTATTGGCCAAATGC
CTGTTTTGATGAGTTGATTTATAAAGATTTTTGTTAAGCTCAGGATTTTAAATTACACAGTTCACAAACAGTAAAGGCCATGTGAAGAGAATTATTACAT
CTTTATTAACCTCAGCATTTACTTTGTTTCTTTTGCTTAGGAAATTGCTCATAATCTGGTTATAATTTTGGTCCAAATTCTTTATTCTTCCTTGAGCTAA
GCAGAATAATGGAATATAATATGTCTTCATAATATAACAACACTAATACACTAATAGTAAGATTAAGTTAGGCAGTCTTCTACCAAATGTGTAATGGAGA
TTGCCTCAAAATTGTGTCCACATAATCCACGCTCATCTTGCAAAGCGCTATTTCAGGCACATCATTGGAATACAGGAAGTAGCCCTGCACCTGCCAGTGA
GCTCGCCATTCACTGATTGGAAGAGTGACCTGGCATCTTGGAAATCATTGTGTGTCTTCAGGAGAATGTGCAGTGTCTTGTAACAACTAATTATAATGCA
AATTAGGGCTACATTGTAATCTGCTTTGTTAATGAAAATGATAAAACAGAATATTGACAAGCTAGGACACCTGTGGTATCTTTAATTGTATCTCCTTCAG
AAGTTTGCTTCTTATGGTATAATAAAGTATGGAAGAATATTGAGTA
- refseq_DSTN.F1 (forward primer)
- refseq_DSTN.R1 (reverse primer)
- Single exon skipping
Frame 0
GKLPSETLDSRRGGVAWPAGCVATAGGA*RAQPRSVLRRARPGVSSRRRVSSALGLSVPQP*GGRSA
YSLPAGSLPRVPATAAAKMIFSSPTRGKDVREL
PAY*EEIHRHYENGV*KKVRALGSVGPGFRASG
VQVADEVCRIFYDMKVRKCSTPEEIKKRKKAVIF
CLSADKKCIIVEEGKEILVGDVGVTITDPFKHF
VGMLPEKDCRYALYDASFETKESRKEELMFFLW
APELAPLKSKMIYASSKDAIKKKFQGIKHECQAN
GPEDLNRACIAEKLGGSLIVAFEGCPV*IIQCH
KLKASMFNVILLLYK*SKYI*ARVSLRGSCLVI
F*SKLFYKHMQTALNKSKV*SFIDVKLNSYWPNA
CFDELIYKDFC*AQDFKLHSSQTVKAM*RELLH
LY*PQHLLCFFCLGNCS*SGYNFGPNSLFFLEL
SRIMEYNMSS*YNNTNTLIVRLS*AVFYQMCNGD
CLKIVST*STLILQSAISGTSLEYRK*PCTCQ*
ARHSLIGRVTWHLGNHCVSSGECAVSCNN*L*C
KLGLHCNLLC**K**NRILTS*DTCGIFNCISFR
SLLLMV**SMEEY*V
Frame 1
GSSPPRLWIAAGGALPGRLVA*QRQAVRDVRSRVPS*GAPAPG*ARAAASAQRWVSRSRSREEDGL
HTRCPPAPSPASLRPPRRR*FSVHPLEAKT*ESY
QRIERRYTGTTRMEFRRRSEPWAVLGQALGPQE
CK*LMKYVAFFMT*KFVNAPHQKKSRKERRLSF
FVSVQTKSASL*KKAKRSWLEMLV*P*LILSSIL
WECFLKKIVAMLCMMQALKQKNPEKKS*CFFCG
HQN*HL*KVK*SMQAPRMQLKRNFKA*NMNVKQ
MDQKISIGLVLLKS*VDP*L*PLKDALCRLFSAT
N*KLPCLMLSSCYINKANIFRPGSH*GGAVLSS
FRVNYSINICKQP*INLKSKVLLM*N*ILIGQM
PVLMS*FIKIFVKLRILNYTVHKQ*RPCEENYYI
FINLSIYFVSFA*EIAHNLVIILVQILYSSLS*
AE*WNIICLHNITTLIH***D*VRQSSTKCVME
IASKLCPHNPRSSCKALFQAHHWNTGSSPAPASE
LAIH*LEE*PGILEIIVCLQENVQCLVTTNYNA
N*GYIVICFVNENDKTEY*QARTPVVSLIVSPS
EVCFLWYNKVWKNIE
Frame 2
EAPLRDSG*PPGGRCLAGWLRSNGRRCVTCAAAFRPEARPPRGKLAPPRQLSAGSLGPAAVRRTVC
ILAARRLPPPRPCDRRGEDDFQFTH*RQRRKRA
TSVLRGDTQALREWSLEEGQSPGQCWARL*GLRS
ASS**SMSHFL*HESS*MLHTRRNQEKKEGCHF
LSQCRQKVHHCRRRQRDLGWRCWCNHN*SFQAF
CGNAS*KRLSLCFV*CKL*NKRIQKRRVDVFFVG
TRTSTSEK*NDLCKLQGCN*KEISRHKT*MSSK
WTRRSQSGLYC*KVRWILNCSL*RMPCVDYSVP
QIESFHV*CYPLAI*IKQIYLGQGLTEGELSCHL
LE*TIL*TYANSPK*I*SLKFY*CEIKFLLAKC
LF**VDL*RFLLSSGF*ITQFTNSKGHVKRIIT
SLLTSAFTLFLLLRKLLIIWL*FWSKFFILP*AK
QNNGI*YVFII*QH*YTNSKIKLGSLLPNV*WR
LPQNCVHIIHAHLAKRYFRHIIGIQEVALHLPV
SSPFTDWKSDLASWKSLCVFRRMCSVL*QLIIMQ
IRATL*SALLMKMIKQNIDKLGHLWYL*LYLLQ
KFASYGIIKYGRILS
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