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Sequence NM_139003
CTAAAGTTCTGAAAGACCTGTTGCTTTTCACCAGGAAGTTTTACTGGGCATCTCCTGAGCCTAGGCAATAGCTGTAGGGTGACTTCTGGAGCCATCCCCGTTTCCCCGCCCCCCAAAAGAAGCGGAGATTTAACGGGGACGTGCGGCCAGAGCTGGGGAAATGGGCCCGCGAGCCAGGCCGGCGCTTCTCCTCCTGATGC
TTTTGCAGACCGCGGTCCTGCAGGGGCGCTTGCTGCGTTCACACTCTCTGCACTACCTCTTCATGGGTGCCTCAGAGCAGGACCTTGGTCTTTCCTTGTT
TGAAGCTTTGGGCTACGTGGATGACCAGCTGTTCGTGTTCTATGATCATGAGAGTCGCCGTGTGGAGCCCCGAACTCCATGGGTTTCCAGTAGAATTTCA
AGCCAGATGTGGCTGCAGCTGAGTCAGAGTCTGAAAGGGTGGGATCACATGTTCACTGTTGACTTCTGGACTATTATGGAAAATCACAACCACAGCAAGG
TGACCACTCTACGGTGTCGGGCCTTGAACTACTACCCCCAGAACATCACCATGAAGTGGCTGAAGGATAAGCAGCCAATGGATGCCAAGGAGTTCGAACC
TAAAGACGTATTGCCCAATGGGGATGGGACCTACCAGGGCTGGATAACCTTGGCTGTACCCCCTGGGGAAGAGCAGAGATATACGTGCCAGGTGGAGCAC
CCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGAGCCCTCACCGTCTGGCACCCTAGTCATTGGAGTCATCAGTGGAATTGCTGTTTTTGTCGTCATCT
TGTTCATTGGAATTTTGTTCATAATATTAAGGAAGAGGCAGGGTTCAAGAGGAGCCATGGGGCACTACGTCTTAGCTGAACGTGAGTGACACGCAGCCTG
CAGACTCACTGTGGGAAGGAGACAAAACTAGAGACTCAAAGAGGGAGTGCATTTATGAGCTCTTCATGTTTCAGGAGAGAGTTGAACCTAAACATAGAAA
TTGCCTGACGAACTCCTTGATTTTAGCCTTCTCTGTTCATTTCCTCAAAAAGATTTCCCCATTTAGGTTTCTGAGTTCCTGCATGCCGGTGATCCCTAGC
TGTGACCTCTCCCCTGGAACTGTCTCTCATGAACCTCAAGCTGCATCTAGAGGCTTCCTTCATTTCCTCCGTCACCTCAGAGACATACACCTATGTCATT
TCATTTCCTATTTTTGGAAGAGGACTCCTTAAATTTGGGGGACTTACATGATTCATTTTAACATCTGAGAAAAGCTTTGAACCCTGGGACGTGGCTAGTC
ATAACCTTACCAGATTTTTACACATGTATCTATGCATTTTCTGGACCCGTTCAACTTTTCCTTTGAATCCTCTCTCTGTGTTACCCAGTAACTCATCTGT
CACCAAGCCTTGGGGATTCTTCCATCTGATTGTGATGTGAGTTGCACAGCTATGAAGGCTGTACACTGCACGAATGGAAGAGGCACCTGTCCCAGAAAAA
GCATCATGGCTATCTGTGGGTAGTATGATGGGTGTTTTTAGCAGGTAGGAGGCAAATATCTTGAAAGGGGTTGTGAAGAGGTGTTTTTTCTAATTGGCAT
GAAGGTGTCATACAGATTTGCAAAGTTTAATGGTGCCTTCATTTGGGATGCTACTCTAGTATTCCAGACCTGAAGAATCACAATAATTTTCTACCTGGTC
TCTCCTTGTTCTGATAATGAAAATTATGATAAGGATGATAAAAGCACTTACTTCGTGTCCGACTCTTCTGAGCACCTACTTACATGCATTACTGCATGCA
CTTCTTACAATAATTCTATGAGATAGGTACTATTATCCCCATTTCTTTTTTAAATGAAGAAAGTGAAGTAGGCCGGGCACGGTGGCTCACGCCTGTAATC
CCAG
- refseq_HFE.F5 (forward primer)
- refseq_HFE.R3 (reverse primer)
- Alternative acceptor
Frame 0
LKF*KTCCFSPGSFTGHLLSLGNSCRVTSGAIPVSPPPKRSGDLTGTCGQSWGNGPASQAGASPPDA
FADRGPAGALAAFTLSALPLHGCLRAGPWSFLV
*SFGLRG*PAVRVL*S*ESPCGAPNSMGFQ*NF
KPDVAAAESESERVGSHVHC*LLDYYGKSQPQQG
DHSTVSGLELLPPEHHHEVAEG*AANGCQGVRT
*RRIAQWGWDLPGLDNLGCTPWGRAEIYVPGGA
PRPGSAPHCDLGALTVWHPSHWSHQWNCCFCRHL
VHWNFVHNIKEEAGFKRSHGALRLS*T*VTRSL
QTHCGKETKLETQRGSAFMSSSCFRRELNLNIE
IA*RTP*F*PSLFISSKRFPHLGF*VPACR*SLA
VTSPLELSLMNLKLHLEASFISSVTSETYTYVI
SFPIFGRGLLKFGGLT*FILTSEKSFEPWDVAS
HNLTRFLHMYLCIFWTRSTFPLNPLSVLPSNSSV
TKPWGFFHLIVM*VAQL*RLYTARMEEAPVPEK
ASWLSVGSMMGVFSR*EANILKGVVKRCFF*LA
*RCHTDLQSLMVPSFGMLL*YSRPEESQ*FSTWS
LLVLIMKIMIRMIKALTSCPTLLSTYLHALLHA
LLTIIL*DRYYYPHFFFK*RK*SRPGTVAHACN
P
Frame 1
*SSERPVAFHQEVLLGIS*A*AIAVG*LLEPSPFPRPPKEAEI*RGRAARAGEMGPRARPALLLLM
LLQTAVLQGRLLRSHSLHYLFMGASEQDLGLSLF
EALGYVDDQLFVFYDHESRRVEPRTPWVSSRIS
SQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSK
VTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEP
KDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEH
PGLDQPLIVIWEPSPSGTLVIGVISGIAVFVVI
LFIGILFIILRKRQGSRGAMGHYVLAERE*HAAC
RLTVGRRQN*RLKEGVHL*ALHVSGES*T*T*K
LPDELLDFSLLCSFPQKDFPI*VSEFLHAGDP*
L*PLPWNCLS*TSSCI*RLPSFPPSPQRHTPMSF
HFLFLEEDSLNLGDLHDSF*HLRKALNPGTWLV
ITLPDFYTCIYAFSGPVQLFL*ILSLCYPVTHL
SPSLGDSSI*L*CELHSYEGCTLHEWKRHLSQKK
HHGYLWVV*WVFLAGRRQIS*KGL*RGVFSNWH
EGVIQICKV*WCLHLGCYSSIPDLKNHNNFLPG
LSLF***KL**G**KHLLRVRLF*APTYMHYCMH
FLQ*FYEIGTIIPISFLNEESEVGRARWLTPVI
P
Frame 2
KVLKDLLLFTRKFYWASPEPRQ*L*GDFWSHPRFPAPQKKRRFNGDVRPELGKWAREPGRRFSS*C
FCRPRSCRGACCVHTLCTTSSWVPQSRTLVFPC
LKLWATWMTSCSCSMIMRVAVWSPELHGFPVEFQ
ARCGCS*VRV*KGGITCSLLTSGLLWKITTTAR
*PLYGVGP*TTTPRTSP*SG*RISSQWMPRSSN
LKTYCPMGMGPTRAG*PWLYPLGKSRDIRARWST
QAWISPSL*SGSPHRLAP*SLESSVELLFLSSS
CSLEFCS*Y*GRGRVQEEPWGTTS*LNVSDTQP
ADSLWEGDKTRDSKRECIYELFMFQERVEPKHRN
CLTNSLILAFSVHFLKKISPFRFLSSCMPVIPS
CDLSPGTVSHEPQAASRGFLHFLRHLRDIHLCH
FISYFWKRTP*IWGTYMIHFNI*EKL*TLGRG*S
*PYQIFTHVSMHFLDPFNFSFESSLCVTQ*LIC
HQALGILPSDCDVSCTAMKAVHCTNGRGTCPRK
SIMAICG*YDGCF*QVGGKYLERGCEEVFFLIGM
KVSYRFAKFNGAFIWDATLVFQT*RITIIFYLV
SPCSDNENYDKDDKSTYFVSDSSEHLLTCITAC
TSYNNSMR*VLLSPFLF*MKKVK*AGHGGSRL*S
Q
Sequence NM_139004
CTAAAGTTCTGAAAGACCTGTTGCTTTTCACCAGGAAGTTTTACTGGGCATCTCCTGAGCCTAGGCAATAGCTGTAGGGTGACTTCTGGAGCCATCCCCGTTTCCCCGCCCCCCAAAAGAAGCGGAGATTTAACGGGGACGTGCGGCCAGAGCTGGGGAAATGGGCCCGCGAGCCAGGCCGGCGCTTCTCCTCCTGATGC
TTTTGCAGACCGCGGTCCTGCAGGGGCGCTTGCTGCGTTCACACTCTCTGCACTACCTCTTCATGGGTGCCTCAGAGCAGGACCTTGGTCTTTCCTTGTT
TGAAGCTTTGGGCTACGTGGATGACCAGCTGTTCGTGTTCTATGATCATGAGAGTCGCCGTGTGGAGCCCCGAACTCCATGGGTTTCCAGTAGAATTTCA
AGCCAGATGTGGCTGCAGCTGAGTCAGAGTCTGAAAGGGTGGGATCACATGTTCACTGTTGACTTCTGGACTATTATGGAAAATCACAACCACAGCAAGG
TGCCTCCTTTGGTGAAGGTGACACATCATGTGACCTCTTCAGTGACCACTCTACGGTGTCGGGCCTTGAACTACTACCCCCAGAACATCACCATGAAGTG
GCTGAAGGATAAGCAGCCAATGGATGCCAAGGAGTTCGAACCTAAAGACGTATTGCCCAATGGGGATGGGACCTACCAGGGCTGGATAACCTTGGCTGTA
CCCCCTGGGGAAGAGCAGAGATATACGTGCCAGGTGGAGCACCCAGGCCTGGATCAGCCCCTCATTGTGATCTGGGAGCCCTCACCGTCTGGCACCCTAG
TCATTGGAGTCATCAGTGGAATTGCTGTTTTTGTCGTCATCTTGTTCATTGGAATTTTGTTCATAATATTAAGGAAGAGGCAGGGTTCAAGAGGAGCCAT
GGGGCACTACGTCTTAGCTGAACGTGAGTGACACGCAGCCTGCAGACTCACTGTGGGAAGGAGACAAAACTAGAGACTCAAAGAGGGAGTGCATTTATGA
GCTCTTCATGTTTCAGGAGAGAGTTGAACCTAAACATAGAAATTGCCTGACGAACTCCTTGATTTTAGCCTTCTCTGTTCATTTCCTCAAAAAGATTTCC
CCATTTAGGTTTCTGAGTTCCTGCATGCCGGTGATCCCTAGCTGTGACCTCTCCCCTGGAACTGTCTCTCATGAACCTCAAGCTGCATCTAGAGGCTTCC
TTCATTTCCTCCGTCACCTCAGAGACATACACCTATGTCATTTCATTTCCTATTTTTGGAAGAGGACTCCTTAAATTTGGGGGACTTACATGATTCATTT
TAACATCTGAGAAAAGCTTTGAACCCTGGGACGTGGCTAGTCATAACCTTACCAGATTTTTACACATGTATCTATGCATTTTCTGGACCCGTTCAACTTT
TCCTTTGAATCCTCTCTCTGTGTTACCCAGTAACTCATCTGTCACCAAGCCTTGGGGATTCTTCCATCTGATTGTGATGTGAGTTGCACAGCTATGAAGG
CTGTACACTGCACGAATGGAAGAGGCACCTGTCCCAGAAAAAGCATCATGGCTATCTGTGGGTAGTATGATGGGTGTTTTTAGCAGGTAGGAGGCAAATA
TCTTGAAAGGGGTTGTGAAGAGGTGTTTTTTCTAATTGGCATGAAGGTGTCATACAGATTTGCAAAGTTTAATGGTGCCTTCATTTGGGATGCTACTCTA
GTATTCCAGACCTGAAGAATCACAATAATTTTCTACCTGGTCTCTCCTTGTTCTGATAATGAAAATTATGATAAGGATGATAAAAGCACTTACTTCGTGT
CCGACTCTTCTGAGCACCTACTTACATGCATTACTGCATGCACTTCTTACAATAATTCTATGAGATAGGTACTATTATCCCCATTTCTTTTTTAAATGAA
GAAAGTGAAGTAGGCCGGGCACGGTGGCTCACGCCTGTAATCCCAG
- refseq_HFE.F5 (forward primer)
- refseq_HFE.R3 (reverse primer)
- Alternative acceptor
Frame 0
LKF*KTCCFSPGSFTGHLLSLGNSCRVTSGAIPVSPPPKRSGDLTGTCGQSWGNGPASQAGASPPDA
FADRGPAGALAAFTLSALPLHGCLRAGPWSFLV
*SFGLRG*PAVRVL*S*ESPCGAPNSMGFQ*NF
KPDVAAAESESERVGSHVHC*LLDYYGKSQPQQG
ASFGEGDTSCDLFSDHSTVSGLELLPPEHHHEV
AEG*AANGCQGVRT*RRIAQWGWDLPGLDNLGC
TPWGRAEIYVPGGAPRPGSAPHCDLGALTVWHPS
HWSHQWNCCFCRHLVHWNFVHNIKEEAGFKRSH
GALRLS*T*VTRSLQTHCGKETKLETQRGSAFM
SSSCFRRELNLNIEIA*RTP*F*PSLFISSKRFP
HLGF*VPACR*SLAVTSPLELSLMNLKLHLEAS
FISSVTSETYTYVISFPIFGRGLLKFGGLT*FI
LTSEKSFEPWDVASHNLTRFLHMYLCIFWTRSTF
PLNPLSVLPSNSSVTKPWGFFHLIVM*VAQL*R
LYTARMEEAPVPEKASWLSVGSMMGVFSR*EAN
ILKGVVKRCFF*LA*RCHTDLQSLMVPSFGMLL*
YSRPEESQ*FSTWSLLVLIMKIMIRMIKALTSC
PTLLSTYLHALLHALLTIIL*DRYYYPHFFFK*
RK*SRPGTVAHACNP
Frame 1
*SSERPVAFHQEVLLGIS*A*AIAVG*LLEPSPFPRPPKEAEI*RGRAARAGEMGPRARPALLLLM
LLQTAVLQGRLLRSHSLHYLFMGASEQDLGLSLF
EALGYVDDQLFVFYDHESRRVEPRTPWVSSRIS
SQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSK
VPPLVKVTHHVTSSVTTLRCRALNYYPQNITMKW
LKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAV
PPGEEQRYTCQVEHPGLDQPLIVIWEPSPSGTL
VIGVISGIAVFVVILFIGILFIILRKRQGSRGAM
GHYVLAERE*HAACRLTVGRRQN*RLKEGVHL*
ALHVSGES*T*T*KLPDELLDFSLLCSFPQKDF
PI*VSEFLHAGDP*L*PLPWNCLS*TSSCI*RLP
SFPPSPQRHTPMSFHFLFLEEDSLNLGDLHDSF
*HLRKALNPGTWLVITLPDFYTCIYAFSGPVQL
FL*ILSLCYPVTHLSPSLGDSSI*L*CELHSYEG
CTLHEWKRHLSQKKHHGYLWVV*WVFLAGRRQI
S*KGL*RGVFSNWHEGVIQICKV*WCLHLGCYS
SIPDLKNHNNFLPGLSLF***KL**G**KHLLRV
RLF*APTYMHYCMHFLQ*FYEIGTIIPISFLNE
ESEVGRARWLTPVIP
Frame 2
KVLKDLLLFTRKFYWASPEPRQ*L*GDFWSHPRFPAPQKKRRFNGDVRPELGKWAREPGRRFSS*C
FCRPRSCRGACCVHTLCTTSSWVPQSRTLVFPC
LKLWATWMTSCSCSMIMRVAVWSPELHGFPVEFQ
ARCGCS*VRV*KGGITCSLLTSGLLWKITTTAR
CLLW*R*HIM*PLQ*PLYGVGP*TTTPRTSP*S
G*RISSQWMPRSSNLKTYCPMGMGPTRAG*PWLY
PLGKSRDIRARWSTQAWISPSL*SGSPHRLAP*
SLESSVELLFLSSSCSLEFCS*Y*GRGRVQEEP
WGTTS*LNVSDTQPADSLWEGDKTRDSKRECIYE
LFMFQERVEPKHRNCLTNSLILAFSVHFLKKIS
PFRFLSSCMPVIPSCDLSPGTVSHEPQAASRGF
LHFLRHLRDIHLCHFISYFWKRTP*IWGTYMIHF
NI*EKL*TLGRG*S*PYQIFTHVSMHFLDPFNF
SFESSLCVTQ*LICHQALGILPSDCDVSCTAMK
AVHCTNGRGTCPRKSIMAICG*YDGCF*QVGGKY
LERGCEEVFFLIGMKVSYRFAKFNGAFIWDATL
VFQT*RITIIFYLVSPCSDNENYDKDDKSTYFV
SDSSEHLLTCITACTSYNNSMR*VLLSPFLF*MK
KVK*AGHGGSRL*SQ
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