Sequences - Rnomics 📊 Palace

        ☰
    
        Rnomics 📊 Palace
    
                       Back to Rnomics Platform
            
                       Open Session

1 warning:

Access restricted to public data.
Please sign in at Rnomics Platform to access your data.

Navigation

Home

Sequences

Sequence NM_001018095

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKMFPMLSRSIWRITSKPSRLPWI VLMP*TQPWHQIPLACKKPSWM*LWKTATLRIK SEICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K *RWNRPKKPMLR*CER*PRSCTASMRRSCRKNRG STVLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA *RRKTRGSGSWTG*LSAWKRNVINCNFNS*NMK QKCLGS*LILTRKGISSWRRHQPASVSGSDT*MT WCIASRRKSSRWSRRIMNYKAGWTI*QKPRPRL KWKPER*EWAVIFYPGKVENSKRSSTSLYSCSH SLITSCGPLEMYVGGDILDCCNDLGVP*HLAGRS QKHQMS*QCLRRCYK*SPSPNALSPKKNR*F*G TFQL*QFQDVPKLS*EAPRTGRRHHAQARVSQI LSRTLSHLINGQLIFQVST*TTSQRYLPSAW*GH AGAEYFLYKPTNS**PHECSHKGKGRQAKEPAS SDVTSAL*RSCGRPLLPVRVCFRNFNKARFGSK KTPFFRENGDSRSCPGVTAVRGSGASSGACRGSR VPAAAPASARAECARCPAASSPKLPRTWRSGVW WSCGKC*SARRDFCAT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCFPCSAGVSGESHPNPVVCPG SF*CHELSLGIRFHWPAKNPRGCDFGKQQH*GSN QKSAADV*SIHGQAEGKAEAVGGSAS*KPAAKN EGGIVPRSQC*GDARDDQEAVQPV*GEAAGRTE EAQC*EGGSFGRNQ*FSESD*RSQ*KDASSRDQP RGERPEDRGAGQAD*AHGKGTSSTATSTPRT*N RNVWGVN*F*QGKVSAVGGGISQPP*ADQTPR* HGALPAEESQADGRGE**TTKQVGLFNRNPGQD* SGNQRDRSGL*SSTQAK*KIRKGPQRVCTAAPT PSSPLVGLWKCMLEETFWIVAMTWECHST*QAE ARNTKCLNNA*GGATNEVLLQMH*APRKIADSEE PSNFNNFRTSLS*ARRHHAQAGGTTHRPESPKS CPALSPTLLMASSSFKFRLEPLLRDIFPVLGEV TLALNTFCINRRTADDRMNAHTRGKGAKPRSRRH PT*LPPFNAPADAPFSQSVFVSGISIKLDSARR RPRSSGKMATPARAPESPPSADPALVAGPAEEA ECPPPRQPQPAQNVLAAPRLRAPSSRGLGAAEFG GAAGNVEAPGETFAQR

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVSHAQQEYLENHIQTQSSALD RFNAMNSALASDSIGLQKTLVDVTLENSNIKDQ IRNLQQTYEASMDKLREKQRQLEVAQVENQLLKM KVESSQEANAEVMREMTKKLYSQYEEKLQEEQR KHSAEKEALLEETNSFLKAIEEANKKMQAAEIS LEEKDQRIGELDRLIERMEKERHQLQLQLLEHET EMSGELTDSDKERYQQLEEASASLRERIRHLDD MVHCQQKKVKQMVEENNELQSRLDYLTETQAKT EVETREIGVGCDLLPRQSRKFEKVLNEFVQLLPL PHHLLWAFGNVCWRRHFGLLQ*LGSAIAPSRQK PETPNVLTMLEAVLQMKSFSKCIEPQEKSLILR NLPTLTISGRP*AELGGTTHRPEAPRTGQSLPNP VPHSLPPY*WPAHLSSFDLNHFSEISSQCLVRS RWR*ILFV*TDEQLMTA*MLTQGERAPSQGAGV IRRNFRPLTLLRTPPSPSPCLFPEFQ*SSIRLEE DPVLPGKWRLPLVPRSHRRPRIRR**RGLPRKP SARRRASLSPRRMCSLPRGFEPQAPEDLAQRSL VELREMLKRQERLLRN

Sequence NM_001018091

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA GATATCTTTCTTAGAAGGAGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA GTGGGCTGTGATCTTCTACCCAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGG GGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGT CATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAA AGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATT GTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTA TCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATG AAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAA TGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTT TTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCA ATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCA CCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCA GTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTC TCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAA TGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTC CTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTT GATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACAT TTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATAT ATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTC AGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCT CTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAATATATC CAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGT GAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCT CTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCA GTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTAT TTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTT AATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCC CCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTC AATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAA ACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACAC TCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTA CATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K RYLS*KERIMNYKAGWTI*QKPRPRLKWKPER*E WAVIFYPGDASKARSAKIS*KTEY*NAEL*SRR GVFREIPRSKG*R*RLVL**ILKIIS*ITDVEM SSSYIRLSN*YQDQCQILLREVIL*SYTKVVIKK AQFVFQKVTFMCLKSLIFEYCV*PHGIKILQYI VYWSDILVYSRTYFFFL*AK*KEVTLLFSFFLP IK*HLLHVFQ*NT*LFQAPKIN*EDIVPFFFG** REQSKELYACLHGVIETLDVKYLGRHVWRMLNK CSSPFSVFP*EERYLSSD*EGLPF*VDAGKWRF FLLVCLFGFVFFFEMGSCSVAQAGVQWQDLGSLQ SRWRSF*ITSCYFRQIWYRRSPS*LSWVKACAS PGLSSLLSQMIGSCTQH*CLIRKAFFFC*N*IV STLLFSPQIGIYRLQKLQSHI*TFMSF*RIIWLS PEKLTYIIKKFFV*YIGVFKSVEE*NIN*NNFK C*GDVLPGLN*IQLFPFEKDCSHLLPGVIIAFI PYPPQANVKSILT*DSH*F*FLKRIVNDFTA*NL MLFLEAG*YGRVHVPGHTVPCNPAI*GDKLC*H FDVVFLLIFLCIVMHLYSQK*NHNVHCFVVCSI YCQYVPILNILLFRQL*DILFHGYTITLRFISYS AI*LCIIM*SVNSDMINILITSIENDLFPEDTA LKMKLLGESVFENFYIYYSLQRSCSFTFPLAIY PMLRIENYYLKSLQIE*LQKRATFKICICFSYLV KVVFFFIFIGHAIFILLRIAHSYFFCCLLGEFA LFLAYKFLRMSVLCHIL*LFLALFVI*HSLWYF SIEVVFFRSLPLCMCLKVFL*SKNG*MSLFSSSI FLFHFYTYL*KEFKFCLYTLTKSVQVWGEKEIF NPSQMYLVILYKVGI*FYFEEFTNWFSM*NHHF PSD**CYFYRILNSYVSFVS*ANFWAFWVFSTLS IIIALIGVNIW*YTGSPLFF*IL*NITYK*VLK TELNG*SNSCVITN*MQRRSCQHSKISVCPLLT LTVSPRANYPDVW*FSLCPFKNYFTSYVYNNMVY ILLTFDLYKNRIIL

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGD*IIKEKVATETALNTAAFRK DIFLRRRE**TTKQVGLFNRNPGQD*SGNQRDR SGL*SSTQEMQAKLAAQKLAERLNIKMRSYNPEG ESSGRYREVRDEDDDWSSDEF*R*SPKSLTLRC HHLTSDFLTSIKISVRYC*GK*FYKVTQR*L*K KPSLSFRR*LSCA*KV*YLNIVFNHMVLKFCNIL CIGLIF*YIVEHTFFFFKPNEKR*LCFFPFSYL SNSIYYMSFSEILSCSRHLKSIRKT*FPSFLGN EGSSLKNCMHVCMGLLRHWM*NT*EDMYGECLTN ALLLFLSSLRKNVISLLIRKDFPFE*MLESGGF FCWFVCLVLCFFLRWGLALSPRLECSGRISAHC NLGGGLFK*PHVTSGKSGTGEAQAN*AG*RLVHH LA*VPCCHR**VHVHNINV**GKHFFFVKIELS VLYFFPPK*VYIDCRSFSPIYKRSCHFRGLYGC LQRN*LTLSKNSLSNILESLKVLKNKILIRTTSN AKVMFYRDLIKYNFFLSKKTVHICFQV*LLLSF LTPLKQM*KVY*PKILISFSS*NE**MISQRKI *CYFWKLDDMEEFMCLDTQFPVILPSREINSVNI LMLCFCLFFYA*LCIYILKNEIIMYIAL*SALY IVNMFPY*IFCFLDSCRIFYFMGIP*L*DLFPI QQFDYVL*CNL*IVI*LTS*SHP*KMTYFQKIPL *K*NC*VKVFLRIFIYITHCRDHVHLHSH*QYI QCSELKITT*NLCKLNNYRKGLHLKFVFVFLT* *RLCFFSYSLAMLYSFF*ELLIHIFSVACLENLL FFWHISS*ECPSFVIYCNFF*PYLSFNIVCGIS V*KLFSLGVYLCVCA*KYFCNPRMDKCPYFLLV FSYFTFTPIFKRNLNFVYIH*LNQYKFGVKRKFL IHLKCIWLYYIR*GFDFISKNLPIGSVCETTIS PLISNAIFIEY*ILMCLLCLRPISGPSGFFLHC Q*LSL*LVLIFGSTQVLPCSFKFCEI*LTNKYLK QSLMGKATAV*SQTRCRGGLASTPKSLCVPS*H SLFPPELTILTYGNSPSVLLRIILPVMCITIWF TFCSLLTYIKIE*Y

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK ISFLEGENNELQSRLDYLTETQAKTEVETREIG VGCDLLPRRCKQSSQRKN*LKD*ILKCGVIIQK GSLQGDTEK*GMKMTIGPLMNSEDNLLNH*R*DV IILHQTF*LVSRSVSDIVEGSNFIKLHKGSYKK SPVCLSEGDFHVLEKFNI*ILCLTTWY*NFAIY CVLV*YFSI**NILFFSLSQMKRGNFAFFLFLTY QIAFITCLSVKYLVVPGT*NQLGRHSSLLFWVM KGAV*RIVCMFAWGY*DIGCEIPRKTCMENA*Q MLFSFFCLPLGRTLSLF*LGRTSLLSRCWKVEVF FVGLFVWFCVFF*DGVLLCRPGWSAVAGSRLTA I*VEVFLNNLMLLQANLVQEKPKLTELGEGLCI TWLEFLAVTDDRFMYTTLMFDKESIFFLLKLNCQ YFTFFPPNRYI*TAEASVPYINVHVILEDYMVV SREINLHYQKILCLIYWSL*KC*RIKY*LEQLQ MLR*CFTGT*LNTTFSFRKRLFTFASRCNYCFHS LPPSSKCEKYTDLRFSLVLVLKTNSK*FHSVKF DVISGSWMIWKSSCAWTHSSL*SCHLGR*TLLT F*CCVFAYFSMHSYAFIFSKMKS*CTLLCSLLYI LSICSHIKYFAF*TAVGYFISWVYHNFKIYFLF SNLIMYYNVICK**YD*HLNHIHRK*LISRRYR SKNEIVR*KCF*EFLYILLTAEIMFIYIPISNIS NAQN*KLLLKIFAN*IITEKGYI*NLYLFFLLS EGCVFFHIHWPCYIHSFENCSFIFFLLLAWRIC SFFGI*VLKNVRPLSYIVTFFSLICHLT*FVVFQ YRSCFL*ESTFVYVLESIFVIQEWINVLIFF*Y FLISLLHLSLKGI*ILFIYIN*ISTSLG*KGNF *SISNVFGYII*GRDLILFRRIYQLVQYVKPPFP L*LVMLFL*NIKFLCVFCVLGQFLGLLGFFYTV NNYRFNWC*YLVVHRFSLVLLNFVKYNLQIST* NRA*WVKQQLCNHKLDAEEVLPALQNLCVSPLDT HCFPQS*LS*RMVILPLSF*ELFYQLCV*QYGL HFAHF*LI*K*NNT

Sequence NM_001018100

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_001018090

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA GATATCTTTCTTAGAAGGAGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA GTGGGCTGTGATCTTCTACCCAGGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGC GCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACT TCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCA GAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGA GATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATA AAAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAAT ATATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTT ACCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGG TAATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTA ACAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAG GTTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCA CTGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTG CATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAAT TGTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGG TTGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACT TCAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTT CATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAA AATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTT AACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTC TATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCC TATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATA CCGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAAT ATATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTAC TTAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAAT TTGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTA TTTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCT AGTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAA TTTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCA TTTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACA CTGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTAC TTAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTT GACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATG GTTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K RYLS*KERIMNYKAGWTI*QKPRPRLKWKPER*E WAVIFYPGYLFDKMIHLVIARRVGLLFPQKSGG AGISSILMTSQQLGFYHFTICPRSCSP*KNPWH FRNSRNRIMRRCKQSSQRKN*LKD*ILKCGVIIQ KGSLQGDTEK*GMKMTIGPLMNSEDNLLNH*R* DVIILHQTF*LVSRSVSDIVEGSNFIKLHKGSY KKSPVCLSEGDFHVLEKFNI*ILCLTTWY*NFAI YCVLV*YFSI**NILFFSLSQMKRGNFAFFLFL TYQIAFITCLSVKYLVVPGT*NQLGRHSSLLFW VMKGAV*RIVCMFAWGY*DIGCEIPRKTCMENA* QMLFSFFCLPLGRTLSLF*LGRTSLLSRCWKVE VFFVGLFVWFCVFF*DGVLLCRPGWSAVAGSRL TAI*VEVFLNNLMLLQANLVQEKPKLTELGEGLC ITWLEFLAVTDDRFMYTTLMFDKESIFFLLKLN CQYFTFFPPNRYI*TAEASVPYINVHVILEDYM VVSREINLHYQKILCLIYWSL*KC*RIKY*LEQL QMLR*CFTGT*LNTTFSFRKRLFTFASRCNYCF HSLPPSSKCEKYTDLRFSLVLVLKTNSK*FHSV KFDVISGSWMIWKSSCAWTHSSL*SCHLGR*TLL TF*CCVFAYFSMHSYAFIFSKMKS*CTLLCSLL YILSICSHIKYFAF*TAVGYFISWVYHNFKIYF LFSNLIMYYNVICK**YD*HLNHIHRK*LISRRY RSKNEIVR*KCF*EFLYILLTAEIMFIYIPISN ISNAQN*KLLLKIFAN*IITEKGYI*NLYLFFL LSEGCVFFHIHWPCYIHSFENCSFIFFLLLAWRI CSFFGI*VLKNVRPLSYIVTFFSLICHLT*FVV FQYRSCFL*ESTFVYVLESIFVIQEWINVLIFF *YFLISLLHLSLKGI*ILFIYIN*ISTSLG*KGN F*SISNVFGYII*GRDLILFRRIYQLVQYVKPP FPL*LVMLFL*NIKFLCVFCVLGQFLGLLGFFY TVNNYRFNWC*YLVVHRFSLVLLNFVKYNLQIST *NRA*WVKQQLCNHKLDAEEVLPALQNLCVSPL DTHCFPQS*LS*RMVILPLSF*ELFYQLCV*QY GLHFAHF*LI*K*NNT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGD*IIKEKVATETALNTAAFRK DIFLRRRE**TTKQVGLFNRNPGQD*SGNQRDR SGL*SSTQVTFSTK*FI*SLPEEWVSYFLRRAAA QG*AAS**HHSSSASTTSPYAHAAALHRRILGT SETAETEL*GDASKARSAKIS*KTEY*NAEL*S RRGVFREIPRSKG*R*RLVL**ILKIIS*ITDVE MSSSYIRLSN*YQDQCQILLREVIL*SYTKVVI KKAQFVFQKVTFMCLKSLIFEYCV*PHGIKILQ YIVYWSDILVYSRTYFFFL*AK*KEVTLLFSFFL PIK*HLLHVFQ*NT*LFQAPKIN*EDIVPFFFG **REQSKELYACLHGVIETLDVKYLGRHVWRML NKCSSPFSVFP*EERYLSSD*EGLPF*VDAGKWR FFLLVCLFGFVFFFEMGSCSVAQAGVQWQDLGS LQSRWRSF*ITSCYFRQIWYRRSPS*LSWVKAC ASPGLSSLLSQMIGSCTQH*CLIRKAFFFC*N*I VSTLLFSPQIGIYRLQKLQSHI*TFMSF*RIIW LSPEKLTYIIKKFFV*YIGVFKSVEE*NIN*NN FKC*GDVLPGLN*IQLFPFEKDCSHLLPGVIIAF IPYPPQANVKSILT*DSH*F*FLKRIVNDFTA* NLMLFLEAG*YGRVHVPGHTVPCNPAI*GDKLC *HFDVVFLLIFLCIVMHLYSQK*NHNVHCFVVCS IYCQYVPILNILLFRQL*DILFHGYTITLRFIS YSAI*LCIIM*SVNSDMINILITSIENDLFPED TALKMKLLGESVFENFYIYYSLQRSCSFTFPLAI YPMLRIENYYLKSLQIE*LQKRATFKICICFSY LVKVVFFFIFIGHAIFILLRIAHSYFFCCLLGE FALFLAYKFLRMSVLCHIL*LFLALFVI*HSLWY FSIEVVFFRSLPLCMCLKVFL*SKNG*MSLFSS SIFLFHFYTYL*KEFKFCLYTLTKSVQVWGEKE IFNPSQMYLVILYKVGI*FYFEEFTNWFSM*NHH FPSD**CYFYRILNSYVSFVS*ANFWAFWVFST LSIIIALIGVNIW*YTGSPLFF*IL*NITYK*V LKTELNG*SNSCVITN*MQRRSCQHSKISVCPLL TLTVSPRANYPDVW*FSLCPFKNYFTSYVYNNM VYILLTFDLYKNRIIL

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK ISFLEGENNELQSRLDYLTETQAKTEVETREIG VGCDLLPRLPFRQNDSSSHCQKSGSPISSEERR RRDKQHLDDITAARLLPLHHMPTQLLSIEESLAL QKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNP EGESSGRYREVRDEDDDWSSDEF*R*SPKSLTL RCHHLTSDFLTSIKISVRYC*GK*FYKVTQR*L* KKPSLSFRR*LSCA*KV*YLNIVFNHMVLKFCN ILCIGLIF*YIVEHTFFFFKPNEKR*LCFFPFS YLSNSIYYMSFSEILSCSRHLKSIRKT*FPSFLG NEGSSLKNCMHVCMGLLRHWM*NT*EDMYGECL TNALLLFLSSLRKNVISLLIRKDFPFE*MLESG GFFCWFVCLVLCFFLRWGLALSPRLECSGRISAH CNLGGGLFK*PHVTSGKSGTGEAQAN*AG*RLV HHLA*VPCCHR**VHVHNINV**GKHFFFVKIE LSVLYFFPPK*VYIDCRSFSPIYKRSCHFRGLYG CLQRN*LTLSKNSLSNILESLKVLKNKILIRTT SNAKVMFYRDLIKYNFFLSKKTVHICFQV*LLL SFLTPLKQM*KVY*PKILISFSS*NE**MISQRK I*CYFWKLDDMEEFMCLDTQFPVILPSREINSV NILMLCFCLFFYA*LCIYILKNEIIMYIAL*SA LYIVNMFPY*IFCFLDSCRIFYFMGIP*L*DLFP IQQFDYVL*CNL*IVI*LTS*SHP*KMTYFQKI PL*K*NC*VKVFLRIFIYITHCRDHVHLHSH*Q YIQCSELKITT*NLCKLNNYRKGLHLKFVFVFLT **RLCFFSYSLAMLYSFF*ELLIHIFSVACLEN LLFFWHISS*ECPSFVIYCNFF*PYLSFNIVCG ISV*KLFSLGVYLCVCA*KYFCNPRMDKCPYFLL VFSYFTFTPIFKRNLNFVYIH*LNQYKFGVKRK FLIHLKCIWLYYIR*GFDFISKNLPIGSVCETT ISPLISNAIFIEY*ILMCLLCLRPISGPSGFFLH CQ*LSL*LVLIFGSTQVLPCSFKFCEI*LTNKY LKQSLMGKATAV*SQTRCRGGLASTPKSLCVPS *HSLFPPELTILTYGNSPSVLLRIILPVMCITIW FTFCSLLTYIKIE*Y

Sequence NM_001018101

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_152451

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_001018096

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_001018093

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA GATATCTTTCTTAGAAGGAGAGGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCG CAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTT CAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAG AAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGAG ATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATAA AAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAATA TATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTA CCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGT AATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTAA CAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGG TTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCAC TGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGC ATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATT GTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGGT TGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACTT CAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTC ATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAAA ATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTA ACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTCT ATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCCT ATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATAC CGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAATA TATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTACT TAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATT TGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTAT TTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCTA GTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAAT TTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCAT TTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACAC TGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTACT TAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTG ACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATGG TTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K RYLS*KERLPFRQNDSSSHCQKSGSPISSEERRR RDKQHLDDITAARLLPLHHMPTQLLSIEESLAL QKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNP EGESSGRYREVRDEDDDWSSDEF*R*SPKSLTLR CHHLTSDFLTSIKISVRYC*GK*FYKVTQR*L* KKPSLSFRR*LSCA*KV*YLNIVFNHMVLKFCN ILCIGLIF*YIVEHTFFFFKPNEKR*LCFFPFSY LSNSIYYMSFSEILSCSRHLKSIRKT*FPSFLG NEGSSLKNCMHVCMGLLRHWM*NT*EDMYGECL TNALLLFLSSLRKNVISLLIRKDFPFE*MLESGG FFCWFVCLVLCFFLRWGLALSPRLECSGRISAH CNLGGGLFK*PHVTSGKSGTGEAQAN*AG*RLV HHLA*VPCCHR**VHVHNINV**GKHFFFVKIEL SVLYFFPPK*VYIDCRSFSPIYKRSCHFRGLYG CLQRN*LTLSKNSLSNILESLKVLKNKILIRTT SNAKVMFYRDLIKYNFFLSKKTVHICFQV*LLLS FLTPLKQM*KVY*PKILISFSS*NE**MISQRK I*CYFWKLDDMEEFMCLDTQFPVILPSREINSV NILMLCFCLFFYA*LCIYILKNEIIMYIAL*SAL YIVNMFPY*IFCFLDSCRIFYFMGIP*L*DLFP IQQFDYVL*CNL*IVI*LTS*SHP*KMTYFQKI PL*K*NC*VKVFLRIFIYITHCRDHVHLHSH*QY IQCSELKITT*NLCKLNNYRKGLHLKFVFVFLT **RLCFFSYSLAMLYSFF*ELLIHIFSVACLEN LLFFWHISS*ECPSFVIYCNFF*PYLSFNIVCGI SV*KLFSLGVYLCVCA*KYFCNPRMDKCPYFLL VFSYFTFTPIFKRNLNFVYIH*LNQYKFGVKRK FLIHLKCIWLYYIR*GFDFISKNLPIGSVCETTI SPLISNAIFIEY*ILMCLLCLRPISGPSGFFLH CQ*LSL*LVLIFGSTQVLPCSFKFCEI*LTNKY LKQSLMGKATAV*SQTRCRGGLASTPKSLCVPS* HSLFPPELTILTYGNSPSVLLRIILPVMCITIW FTFCSLLTYIKIE*Y

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGD*IIKEKVATETALNTAAFRK DIFLRRRGYLFDKMIHLVIARRVGLLFPQKSGG AGISSILMTSQQLGFYHFTICPRSCSP*KNPWHF RNSRNRIMRRCKQSSQRKN*LKD*ILKCGVIIQ KGSLQGDTEK*GMKMTIGPLMNSEDNLLNH*R* DVIILHQTF*LVSRSVSDIVEGSNFIKLHKGSYK KSPVCLSEGDFHVLEKFNI*ILCLTTWY*NFAI YCVLV*YFSI**NILFFSLSQMKRGNFAFFLFL TYQIAFITCLSVKYLVVPGT*NQLGRHSSLLFWV MKGAV*RIVCMFAWGY*DIGCEIPRKTCMENA* QMLFSFFCLPLGRTLSLF*LGRTSLLSRCWKVE VFFVGLFVWFCVFF*DGVLLCRPGWSAVAGSRLT AI*VEVFLNNLMLLQANLVQEKPKLTELGEGLC ITWLEFLAVTDDRFMYTTLMFDKESIFFLLKLN CQYFTFFPPNRYI*TAEASVPYINVHVILEDYMV VSREINLHYQKILCLIYWSL*KC*RIKY*LEQL QMLR*CFTGT*LNTTFSFRKRLFTFASRCNYCF HSLPPSSKCEKYTDLRFSLVLVLKTNSK*FHSVK FDVISGSWMIWKSSCAWTHSSL*SCHLGR*TLL TF*CCVFAYFSMHSYAFIFSKMKS*CTLLCSLL YILSICSHIKYFAF*TAVGYFISWVYHNFKIYFL FSNLIMYYNVICK**YD*HLNHIHRK*LISRRY RSKNEIVR*KCF*EFLYILLTAEIMFIYIPISN ISNAQN*KLLLKIFAN*IITEKGYI*NLYLFFLL SEGCVFFHIHWPCYIHSFENCSFIFFLLLAWRI CSFFGI*VLKNVRPLSYIVTFFSLICHLT*FVV FQYRSCFL*ESTFVYVLESIFVIQEWINVLIFF* YFLISLLHLSLKGI*ILFIYIN*ISTSLG*KGN F*SISNVFGYII*GRDLILFRRIYQLVQYVKPP FPL*LVMLFL*NIKFLCVFCVLGQFLGLLGFFYT VNNYRFNWC*YLVVHRFSLVLLNFVKYNLQIST *NRA*WVKQQLCNHKLDAEEVLPALQNLCVSPL DTHCFPQS*LS*RMVILPLSF*ELFYQLCV*QYG LHFAHF*LI*K*NNT

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK ISFLEGEVTFSTK*FI*SLPEEWVSYFLRRAAA QG*AAS**HHSSSASTTSPYAHAAALHRRILGT SETAETEL*GDASKARSAKIS*KTEY*NAEL*SR RGVFREIPRSKG*R*RLVL**ILKIIS*ITDVE MSSSYIRLSN*YQDQCQILLREVIL*SYTKVVI KKAQFVFQKVTFMCLKSLIFEYCV*PHGIKILQY IVYWSDILVYSRTYFFFL*AK*KEVTLLFSFFL PIK*HLLHVFQ*NT*LFQAPKIN*EDIVPFFFG **REQSKELYACLHGVIETLDVKYLGRHVWRMLN KCSSPFSVFP*EERYLSSD*EGLPF*VDAGKWR FFLLVCLFGFVFFFEMGSCSVAQAGVQWQDLGS LQSRWRSF*ITSCYFRQIWYRRSPS*LSWVKACA SPGLSSLLSQMIGSCTQH*CLIRKAFFFC*N*I VSTLLFSPQIGIYRLQKLQSHI*TFMSF*RIIW LSPEKLTYIIKKFFV*YIGVFKSVEE*NIN*NNF KC*GDVLPGLN*IQLFPFEKDCSHLLPGVIIAF IPYPPQANVKSILT*DSH*F*FLKRIVNDFTA* NLMLFLEAG*YGRVHVPGHTVPCNPAI*GDKLC* HFDVVFLLIFLCIVMHLYSQK*NHNVHCFVVCS IYCQYVPILNILLFRQL*DILFHGYTITLRFIS YSAI*LCIIM*SVNSDMINILITSIENDLFPEDT ALKMKLLGESVFENFYIYYSLQRSCSFTFPLAI YPMLRIENYYLKSLQIE*LQKRATFKICICFSY LVKVVFFFIFIGHAIFILLRIAHSYFFCCLLGEF ALFLAYKFLRMSVLCHIL*LFLALFVI*HSLWY FSIEVVFFRSLPLCMCLKVFL*SKNG*MSLFSS SIFLFHFYTYL*KEFKFCLYTLTKSVQVWGEKEI FNPSQMYLVILYKVGI*FYFEEFTNWFSM*NHH FPSD**CYFYRILNSYVSFVS*ANFWAFWVFST LSIIIALIGVNIW*YTGSPLFF*IL*NITYK*VL KTELNG*SNSCVITN*MQRRSCQHSKISVCPLL TLTVSPRANYPDVW*FSLCPFKNYFTSYVYNNM VYILLTFDLYKNRIIL

Sequence NM_001018097

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGA AACCAGAGAGATAGGAGTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGA GTCCTGGAGTTAACCATGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCC TTTGGGAAGGGTGACTGTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGG CAGAGTGAGCAGGTAAATACCTAGCAGAAGAATGGCTGGGTCAAACGGTATATTTTAAGAAACTGCCAAACTGGTTTCCAAAGAGGTTGTACTATTTTGC ATTCCCATTAGCAGTGTATCAAAGTTCCAGTTGCTCTACATCCTTTACAACACTTGGAATGGTCAGTCTTTCTGGCAAAGTAGAAAATTCGAAAAGGTCC TCAACGAGTTTGTACAGCTGCTCCCACTCCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATTTTGGATTGTTGCAATGACTTGG GAGTGCCATAGCACCTAGCAGGCAGAAGCCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAAGTCCTTCTCCAAATGCATTGAG CCCCAAGAAAAATCGCTGATTCTGAGGAACCTTCCAACTTTAACAATTTCAGGACGTCCCTAAGCTGAGCTAGGAGGCACCACGCACAGGCCGGAGGCAC CACGCACAGGCCAGAGTCTCCCAAATCCTGTCCCGCACTCTCTCCCACCTTATTAATGGCCAGCTCATCTTTCAAGTTTCGACTTGAACCACTTCTCAGA GATATCTTCCCAGAAAATTCATTTGCAAATTGCCCGACAAAGGTAAAAAGATCTTTGACTCTTTTGCCAAACTGAAAGCTGCCATTGCAGAATGTGAAGA AGTTAGAAGAAAAAGTGAACTGTTTAACCCTGTTAGTTTAGACTGTAAGCTAAGGCAAAAAGCAATTGCAGAAGTTGATGTGGGTACAGATAAGGCCCAG AATTCTGACCCGATACTTGATACTTCATCACTAGTTCCTGGATGTTCCTCTGTAGATAACATCAAGTCATCTCAAACCTCACAAAACCAGGGACTTGGAC GTCCTACTCTTGAAGGTGATGAAGAGACTTCAGAGGTTGAGTACACAGTGAATAAGGGCCCAGCTTCCAGCAATAGAGACAGGGTACCACCTTCATCTGA AGCTAGTGAGCATCACCCGCGGCATCGTGTTTCAAGTCAAGCGGAAGATACTTCCAGCAGCTTTGACAACCTGTTTATTGACAGGTTACAGAGGATCACC ATTGCGGACCAAGGTGAACAACAGTCAGAAGAAAACGCAAGTACTAAGAACTTGACAGGCCTTTCCAGTGGGACTGAGAAGAAACCTCATTACATGGAAG TGCTAGAAATGCGAGCCAAAAACCCAGTGCCCCAGCTGCGTAAATTTAAAACCAATGTGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAA GAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCC ACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTG AAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGA ATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGG AAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATA TTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAA TGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAA TCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGT GAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAA GGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCG CCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAG AAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTG ATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATA TATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAA AAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGAC TGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGT TCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCC CTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAA TGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTAT TTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAAT CACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTG CAGAGATCATGTTCATTTACATTCCCATTAGCAATATATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAA GGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGC TCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTT TTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGT AATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATT AACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAA GAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTT AGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTT TTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCT TGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTA AGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRIMNYKAGWTI*QKPRPRLKW KPER*EWAVIFYPAKQAGLVKL*CLLGTTPHTQE SWS*P*RKL*LRHSEAYTFYRWTKALEPCGFKS FGKGDCCFPYTQCKPEWESLRL*STSKTGRKVK AE*AGKYLAEEWLGQTVYFKKLPNWFPKRLYYFA FPLAVYQSSSCSTSFTTLGMVSLSGKVENSKRS STSLYSCSHSLITSCGPLEMYVGGDILDCCNDL GVP*HLAGRSQKHQMS*QCLRRCYK*SPSPNALS PKKNR*F*GTFQL*QFQDVPKLS*EAPRTGRRH HAQARVSQILSRTLSHLINGQLIFQVST*TTSQ RYLPRKFICKLPDKGKKIFDSFAKLKAAIAECEE VRRKSELFNPVSLDCKLRQKAIAEVDVGTDKAQ NSDPILDTSSLVPGCSSVDNIKSSQTSQNQGLG RPTLEGDEETSEVEYTVNKGPASSNRDRVPPSSE ASEHHPRHRVSSQAEDTSSSFDNLFIDRLQRIT IADQGEQQSEENASTKNLTGLSSGTEKKPHYME VLEMRAKNPVPQLRKFKTNVLPFRQNDSSSHCQK SGSPISSEERRRRDKQHLDDITAARLLPLHHMP TQLLSIEESLALQKQQKQNYEEMQAKLAAQKLA ERLNIKMRSYNPEGESSGRYREVRDEDDDWSSDE F*R*SPKSLTLRCHHLTSDFLTSIKISVRYC*G K*FYKVTQR*L*KKPSLSFRR*LSCA*KV*YLN IVFNHMVLKFCNILCIGLIF*YIVEHTFFFFKPN EKR*LCFFPFSYLSNSIYYMSFSEILSCSRHLK SIRKT*FPSFLGNEGSSLKNCMHVCMGLLRHWM *NT*EDMYGECLTNALLLFLSSLRKNVISLLIRK DFPFE*MLESGGFFCWFVCLVLCFFLRWGLALS PRLECSGRISAHCNLGGGLFK*PHVTSGKSGTG EAQAN*AG*RLVHHLA*VPCCHR**VHVHNINV* *GKHFFFVKIELSVLYFFPPK*VYIDCRSFSPI YKRSCHFRGLYGCLQRN*LTLSKNSLSNILESL KVLKNKILIRTTSNAKVMFYRDLIKYNFFLSKKT VHICFQV*LLLSFLTPLKQM*KVY*PKILISFS S*NE**MISQRKI*CYFWKLDDMEEFMCLDTQF PVILPSREINSVNILMLCFCLFFYA*LCIYILKN EIIMYIAL*SALYIVNMFPY*IFCFLDSCRIFY FMGIP*L*DLFPIQQFDYVL*CNL*IVI*LTS* SHP*KMTYFQKIPL*K*NC*VKVFLRIFIYITHC RDHVHLHSH*QYIQCSELKITT*NLCKLNNYRK GLHLKFVFVFLT**RLCFFSYSLAMLYSFF*EL LIHIFSVACLENLLFFWHISS*ECPSFVIYCNFF *PYLSFNIVCGISV*KLFSLGVYLCVCA*KYFC NPRMDKCPYFLLVFSYFTFTPIFKRNLNFVYIH *LNQYKFGVKRKFLIHLKCIWLYYIR*GFDFISK NLPIGSVCETTISPLISNAIFIEY*ILMCLLCL RPISGPSGFFLHCQ*LSL*LVLIFGSTQVLPCS FKFCEI*LTNKYLKQSLMGKATAV*SQTRCRGGL ASTPKSLCVPS*HSLFPPELTILTYGNSPSVLL RIILPVMCITIWFTFCSLLTYIKIE*Y

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGE**TTKQVGLFNRNPGQD*SG NQRDRSGL*SSTQPNRQDS*NCDAF*ELHPIHK SPGVNHEENSDLGTQRHTLFTDGQKLWNPVASNP LGRVTVVSPTHSVSRNGNR*GSDPLLRQEGK*R QSEQVNT*QKNGWVKRYILRNCQTGFQRGCTIL HSH*QCIKVPVALHPLQHLEWSVFLAK*KIRKGP QRVCTAAPTPSSPLVGLWKCMLEETFWIVAMTW ECHST*QAEARNTKCLNNA*GGATNEVLLQMH* APRKIADSEEPSNFNNFRTSLS*ARRHHAQAGGT THRPESPKSCPALSPTLLMASSSFKFRLEPLLR DIFPENSFANCPTKVKRSLTLLPN*KLPLQNVK KLEEKVNCLTLLV*TVS*GKKQLQKLMWVQIRPR ILTRYLILHH*FLDVPL*ITSSHLKPHKTRDLD VLLLKVMKRLQRLSTQ*IRAQLPAIETGYHLHL KLVSITRGIVFQVKRKILPAALTTCLLTGYRGSP LRTKVNNSQKKTQVLRT*QAFPVGLRRNLITWK C*KCEPKTQCPSCVNLKPMCYLFDKMIHLVIAR RVGLLFPQKSGGAGISSILMTSQQLGFYHFTICP RSCSP*KNPWHFRNSRNRIMRRCKQSSQRKN*L KD*ILKCGVIIQKGSLQGDTEK*GMKMTIGPLM NSEDNLLNH*R*DVIILHQTF*LVSRSVSDIVEG SNFIKLHKGSYKKSPVCLSEGDFHVLEKFNI*I LCLTTWY*NFAIYCVLV*YFSI**NILFFSLSQ MKRGNFAFFLFLTYQIAFITCLSVKYLVVPGT*N QLGRHSSLLFWVMKGAV*RIVCMFAWGY*DIGC EIPRKTCMENA*QMLFSFFCLPLGRTLSLF*LG RTSLLSRCWKVEVFFVGLFVWFCVFF*DGVLLCR PGWSAVAGSRLTAI*VEVFLNNLMLLQANLVQE KPKLTELGEGLCITWLEFLAVTDDRFMYTTLMF DKESIFFLLKLNCQYFTFFPPNRYI*TAEASVPY INVHVILEDYMVVSREINLHYQKILCLIYWSL* KC*RIKY*LEQLQMLR*CFTGT*LNTTFSFRKR LFTFASRCNYCFHSLPPSSKCEKYTDLRFSLVLV LKTNSK*FHSVKFDVISGSWMIWKSSCAWTHSS L*SCHLGR*TLLTF*CCVFAYFSMHSYAFIFSK MKS*CTLLCSLLYILSICSHIKYFAF*TAVGYFI SWVYHNFKIYFLFSNLIMYYNVICK**YD*HLN HIHRK*LISRRYRSKNEIVR*KCF*EFLYILLT AEIMFIYIPISNISNAQN*KLLLKIFAN*IITEK GYI*NLYLFFLLSEGCVFFHIHWPCYIHSFENC SFIFFLLLAWRICSFFGI*VLKNVRPLSYIVTF FSLICHLT*FVVFQYRSCFL*ESTFVYVLESIFV IQEWINVLIFF*YFLISLLHLSLKGI*ILFIYI N*ISTSLG*KGNF*SISNVFGYII*GRDLILFR RIYQLVQYVKPPFPL*LVMLFL*NIKFLCVFCVL GQFLGLLGFFYTVNNYRFNWC*YLVVHRFSLVL LNFVKYNLQIST*NRA*WVKQQLCNHKLDAEEV LPALQNLCVSPLDTHCFPQS*LS*RMVILPLSF* ELFYQLCV*QYGLHFAHF*LI*K*NNT

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEENNELQSRLDYLTETQAKTEVE TREIGVGCDLLPSQTGRTREIVMPSRNYTPYTR VLELTMKKTLT*ALRGIHFLQMDKSSGTLWLQI LWEG*LLFPLHTV*AGMGIAEALIHF*DRKESEG RVSR*IPSRRMAGSNGIF*ETAKLVSKEVVLFC IPISSVSKFQLLYILYNTWNGQSFWQSRKFEKV LNEFVQLLPLPHHLLWAFGNVCWRRHFGLLQ*LG SAIAPSRQKPETPNVLTMLEAVLQMKSFSKCIE PQEKSLILRNLPTLTISGRP*AELGGTTHRPEA PRTGQSLPNPVPHSLPPY*WPAHLSSFDLNHFSE ISSQKIHLQIARQR*KDL*LFCQTESCHCRM*R S*KKK*TV*PC*FRL*AKAKSNCRS*CGYR*GP EF*PDT*YFITSSWMFLCR*HQVISNLTKPGTWT SYS*R**RDFRG*VHSE*GPSFQQ*RQGTTFI* S**ASPAASCFKSSGRYFQQL*QPVY*QVTEDH HCGPR*TTVRRKRKY*ELDRPFQWD*EETSLHGS ARNASQKPSAPAA*I*NQCVTFSTK*FI*SLPE EWVSYFLRRAAAQG*AAS**HHSSSASTTSPYA HAAALHRRILGTSETAETEL*GDASKARSAKIS* KTEY*NAEL*SRRGVFREIPRSKG*R*RLVL** ILKIIS*ITDVEMSSSYIRLSN*YQDQCQILLR EVIL*SYTKVVIKKAQFVFQKVTFMCLKSLIFEY CV*PHGIKILQYIVYWSDILVYSRTYFFFL*AK *KEVTLLFSFFLPIK*HLLHVFQ*NT*LFQAPK IN*EDIVPFFFG**REQSKELYACLHGVIETLDV KYLGRHVWRMLNKCSSPFSVFP*EERYLSSD*E GLPF*VDAGKWRFFLLVCLFGFVFFFEMGSCSV AQAGVQWQDLGSLQSRWRSF*ITSCYFRQIWYRR SPS*LSWVKACASPGLSSLLSQMIGSCTQH*CL IRKAFFFC*N*IVSTLLFSPQIGIYRLQKLQSH I*TFMSF*RIIWLSPEKLTYIIKKFFV*YIGVFK SVEE*NIN*NNFKC*GDVLPGLN*IQLFPFEKD CSHLLPGVIIAFIPYPPQANVKSILT*DSH*F* FLKRIVNDFTA*NLMLFLEAG*YGRVHVPGHTVP CNPAI*GDKLC*HFDVVFLLIFLCIVMHLYSQK *NHNVHCFVVCSIYCQYVPILNILLFRQL*DIL FHGYTITLRFISYSAI*LCIIM*SVNSDMINILI TSIENDLFPEDTALKMKLLGESVFENFYIYYSL QRSCSFTFPLAIYPMLRIENYYLKSLQIE*LQK RATFKICICFSYLVKVVFFFIFIGHAIFILLRIA HSYFFCCLLGEFALFLAYKFLRMSVLCHIL*LF LALFVI*HSLWYFSIEVVFFRSLPLCMCLKVFL *SKNG*MSLFSSSIFLFHFYTYL*KEFKFCLYTL TKSVQVWGEKEIFNPSQMYLVILYKVGI*FYFE EFTNWFSM*NHHFPSD**CYFYRILNSYVSFVS *ANFWAFWVFSTLSIIIALIGVNIW*YTGSPLFF *IL*NITYK*VLKTELNG*SNSCVITN*MQRRS CQHSKISVCPLLTLTVSPRANYPDVW*FSLCPF KNYFTSYVYNNMVYILLTFDLYKNRIIL

Sequence NM_001018098

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATCATGTCGCACGAGCTCTTCTCCAGATTTAGTCTCCGGCTCTTTGGAAGATGATAAGTTGG TAGCCTGCTCTGGGTTGGAAGCGATTCCTTTATTGTTCGTGATTAGAAGAACACTTGTACACTCTCTGCTGGGAAGAGGTGGTATTTATCTATGATGAGC CAATGAACAAAACAGGAAGGACATATTATTTAAAGGAAAATTTGACCATGTGGGGCTCTTGTTTGTGAAAACACCCACATACTGTCACCCTGACTTGTAC AGCCTCAAACTGCAGAGTCAAGAGAACTCACAGATCCACAGAGTATTTTTAGGAACTTGAGTAGACATTGAAGAAGGAACTGCAATGAAAAAGGCTACTG AAAAACCATTCAGAAAGATCCAGGTGGAGGCTTGGAAGATGAATCCTTACTTATGGAAAAGCTGCCAAAGACATCTGCTAAGGAGATGGGAAGAGAGCTT GAAGAATGTTAGTGCTCTGGGAAGGAGGTGCTCCCACCAGATCACCCCCAAGAAAGTACAGGAGCAGCCAGATTGAATCATTAAAGAAAAAGTTGCAACA GAAACAGCTCTTAATACTGCAGCTTTTAGAAAAGATATCTTTCTTAGAAGGAGAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTA CACCCCATACACAAGAGTCCTGGAGTTAACCATGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACC CTGTGGCTTCAAATCCTTTGGGAAGGGTGACTGTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACA GGAAGGAAAGTGAAGGCAGAGTGAGCAGGGAAGGAAAGGCCTAAAAGGGAGACTAAAGATGAGTTGCTAAAAAAGAGAGAACAGAAGCAAAGAGACCAGA AGGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTG ATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAA TTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTCAGGGAGA TACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGTCATCATCTTACATCAG ACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAAAGCCCAGTTTGTCTTT CAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATTGTGTATTGGTCTGATA TTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTATCAAATAGCATTTATT ACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATGAAGGGAGCAGTCTAAA GAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTT CTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTTTTTTGTTGGTTTGTTT GTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCAATCTAGGTGGAGGTCT TTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCACCTGGCTTGAGTTCCT TGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCAGTACTTTACTTTTTTC CCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTCTCCAGAGAAATTAACT TACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAATGCTAAGGTGATGTTT TACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCTCAAGC AAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTTGATGTTATTTCTGGAA GCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACATTTTGATGTTGTGTTTT TGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATATATTGTCAATATGTTCC CATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTCAGCAATTTGATTATGT ATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCTCTAAAAATGAAATTGT TAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAATATATCCAATGCTCAGAATTGA AAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGTGAAGGTTGTGTTTTTT TTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCTCTTTTTTTGGCATATA AGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCAGTATAGAAGTTGTTTT CTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTATTTTCTTATTTCACTTT TACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTTAATCCATCTCAAATGT ATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCCCCTCTGATTAGTAATG CTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTCAATAATTATCGCTTTA ATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAAACAGAGCTTAATGGGT AAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACACTCACTGTTTCCCCCAG AGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTACATTTTGCTCACTTTT GACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRSCRTSSSPDLVSGSLEDDKL VACSGLEAIPLLFVIRRTLVHSLLGRGGIYL**A NEQNRKDILFKGKFDHVGLLFVKTPTYCHPDLY SLKLQSQENSQIHRVFLGT*VDIEEGTAMKKAT EKPFRKIQVEAWKMNPYLWKSCQRHLLRRWEESL KNVSALGRRCSHQITPKKVQEQPD*IIKEKVAT ETALNTAAFRKDIFLRRRAKQAGLVKL*CLLGT TPHTQESWS*P*RKL*LRHSEAYTFYRWTKALEP CGFKSFGKGDCCFPYTQCKPEWESLRL*STSKT GRKVKAE*AGKERPKRETKDELLKKREQKQRDQ KVTFSTK*FI*SLPEEWVSYFLRRAAAQG*AAS* *HHSSSASTTSPYAHAAALHRRILGTSETAETE L*GDASKARSAKIS*KTEY*NAEL*SRRGVFRE IPRSKG*R*RLVL**ILKIIS*ITDVEMSSSYIR LSN*YQDQCQILLREVIL*SYTKVVIKKAQFVF QKVTFMCLKSLIFEYCV*PHGIKILQYIVYWSD ILVYSRTYFFFL*AK*KEVTLLFSFFLPIK*HLL HVFQ*NT*LFQAPKIN*EDIVPFFFG**REQSK ELYACLHGVIETLDVKYLGRHVWRMLNKCSSPF SVFP*EERYLSSD*EGLPF*VDAGKWRFFLLVCL FGFVFFFEMGSCSVAQAGVQWQDLGSLQSRWRS F*ITSCYFRQIWYRRSPS*LSWVKACASPGLSS LLSQMIGSCTQH*CLIRKAFFFC*N*IVSTLLFS PQIGIYRLQKLQSHI*TFMSF*RIIWLSPEKLT YIIKKFFV*YIGVFKSVEE*NIN*NNFKC*GDV LPGLN*IQLFPFEKDCSHLLPGVIIAFIPYPPQA NVKSILT*DSH*F*FLKRIVNDFTA*NLMLFLE AG*YGRVHVPGHTVPCNPAI*GDKLC*HFDVVF LLIFLCIVMHLYSQK*NHNVHCFVVCSIYCQYVP ILNILLFRQL*DILFHGYTITLRFISYSAI*LC IIM*SVNSDMINILITSIENDLFPEDTALKMKL LGESVFENFYIYYSLQRSCSFTFPLAIYPMLRIE NYYLKSLQIE*LQKRATFKICICFSYLVKVVFF FIFIGHAIFILLRIAHSYFFCCLLGEFALFLAY KFLRMSVLCHIL*LFLALFVI*HSLWYFSIEVVF FRSLPLCMCLKVFL*SKNG*MSLFSSSIFLFHF YTYL*KEFKFCLYTLTKSVQVWGEKEIFNPSQM YLVILYKVGI*FYFEEFTNWFSM*NHHFPSD**C YFYRILNSYVSFVS*ANFWAFWVFSTLSIIIAL IGVNIW*YTGSPLFF*IL*NITYK*VLKTELNG *SNSCVITN*MQRRSCQHSKISVCPLLTLTVSPR ANYPDVW*FSLCPFKNYFTSYVYNNMVYILLTF DLYKNRIIL

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGDHVARALLQI*SPALWKMISW *PALGWKRFLYCS*LEEHLYTLCWEEVVFIYDE PMNKTGRTYYLKENLTMWGSCL*KHPHTVTLTCT ASNCRVKRTHRSTEYF*ELE*TLKKELQ*KRLL KNHSERSRWRLGR*ILTYGKAAKDIC*GDGKRA *RMLVLWEGGAPTRSPPRKYRSSQIESLKKKLQQ KQLLILQLLEKISFLEGEPNRQDS*NCDAF*EL HPIHKSPGVNHEENSDLGTQRHTLFTDGQKLWN PVASNPLGRVTVVSPTHSVSRNGNR*GSDPLLRQ EGK*RQSEQGRKGLKGRLKMSC*KRENRSKETR RLPFRQNDSSSHCQKSGSPISSEERRRRDKQHL DDITAARLLPLHHMPTQLLSIEESLALQKQQKQN YEEMQAKLAAQKLAERLNIKMRSYNPEGESSGR YREVRDEDDDWSSDEF*R*SPKSLTLRCHHLTS DFLTSIKISVRYC*GK*FYKVTQR*L*KKPSLSF RR*LSCA*KV*YLNIVFNHMVLKFCNILCIGLI F*YIVEHTFFFFKPNEKR*LCFFPFSYLSNSIY YMSFSEILSCSRHLKSIRKT*FPSFLGNEGSSLK NCMHVCMGLLRHWM*NT*EDMYGECLTNALLLF LSSLRKNVISLLIRKDFPFE*MLESGGFFCWFV CLVLCFFLRWGLALSPRLECSGRISAHCNLGGGL FK*PHVTSGKSGTGEAQAN*AG*RLVHHLA*VP CCHR**VHVHNINV**GKHFFFVKIELSVLYFF PPK*VYIDCRSFSPIYKRSCHFRGLYGCLQRN*L TLSKNSLSNILESLKVLKNKILIRTTSNAKVMF YRDLIKYNFFLSKKTVHICFQV*LLLSFLTPLK QM*KVY*PKILISFSS*NE**MISQRKI*CYFWK LDDMEEFMCLDTQFPVILPSREINSVNILMLCF CLFFYA*LCIYILKNEIIMYIAL*SALYIVNMF PY*IFCFLDSCRIFYFMGIP*L*DLFPIQQFDYV L*CNL*IVI*LTS*SHP*KMTYFQKIPL*K*NC *VKVFLRIFIYITHCRDHVHLHSH*QYIQCSEL KITT*NLCKLNNYRKGLHLKFVFVFLT**RLCFF SYSLAMLYSFF*ELLIHIFSVACLENLLFFWHI SS*ECPSFVIYCNFF*PYLSFNIVCGISV*KLF SLGVYLCVCA*KYFCNPRMDKCPYFLLVFSYFTF TPIFKRNLNFVYIH*LNQYKFGVKRKFLIHLKC IWLYYIR*GFDFISKNLPIGSVCETTISPLISN AIFIEY*ILMCLLCLRPISGPSGFFLHCQ*LSL* LVLIFGSTQVLPCSFKFCEI*LTNKYLKQSLMG KATAV*SQTRCRGGLASTPKSLCVPS*HSLFPP ELTILTYGNSPSVLLRIILPVMCITIWFTFCSLL TYIKIE*Y

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEIMSHELFSRFSLRLFGR**VG SLLWVGSDSFIVRD*KNTCTLSAGKRWYLSMMS Q*TKQEGHII*RKI*PCGALVCENTHILSP*LV QPQTAESRELTDPQSIFRNLSRH*RRNCNEKGY* KTIQKDPGGGLEDESLLMEKLPKTSAKEMGREL EEC*CSGKEVLPPDHPQESTGAARLNH*RKSCN RNSS*YCSF*KRYLS*KESQTGRTREIVMPSRNY TPYTRVLELTMKKTLT*ALRGIHFLQMDKSSGT LWLQILWEG*LLFPLHTV*AGMGIAEALIHF*D RKESEGRVSREGKA*KGD*R*VAKKERTEAKRPE GYLFDKMIHLVIARRVGLLFPQKSGGAGISSIL MTSQQLGFYHFTICPRSCSP*KNPWHFRNSRNR IMRRCKQSSQRKN*LKD*ILKCGVIIQKGSLQGD TEK*GMKMTIGPLMNSEDNLLNH*R*DVIILHQ TF*LVSRSVSDIVEGSNFIKLHKGSYKKSPVCL SEGDFHVLEKFNI*ILCLTTWY*NFAIYCVLV*Y FSI**NILFFSLSQMKRGNFAFFLFLTYQIAFI TCLSVKYLVVPGT*NQLGRHSSLLFWVMKGAV* RIVCMFAWGY*DIGCEIPRKTCMENA*QMLFSFF CLPLGRTLSLF*LGRTSLLSRCWKVEVFFVGLF VWFCVFF*DGVLLCRPGWSAVAGSRLTAI*VEV FLNNLMLLQANLVQEKPKLTELGEGLCITWLEFL AVTDDRFMYTTLMFDKESIFFLLKLNCQYFTFF PPNRYI*TAEASVPYINVHVILEDYMVVSREIN LHYQKILCLIYWSL*KC*RIKY*LEQLQMLR*CF TGT*LNTTFSFRKRLFTFASRCNYCFHSLPPSS KCEKYTDLRFSLVLVLKTNSK*FHSVKFDVISG SWMIWKSSCAWTHSSL*SCHLGR*TLLTF*CCVF AYFSMHSYAFIFSKMKS*CTLLCSLLYILSICS HIKYFAF*TAVGYFISWVYHNFKIYFLFSNLIM YYNVICK**YD*HLNHIHRK*LISRRYRSKNEIV R*KCF*EFLYILLTAEIMFIYIPISNISNAQN* KLLLKIFAN*IITEKGYI*NLYLFFLLSEGCVF FHIHWPCYIHSFENCSFIFFLLLAWRICSFFGI* VLKNVRPLSYIVTFFSLICHLT*FVVFQYRSCF L*ESTFVYVLESIFVIQEWINVLIFF*YFLISL LHLSLKGI*ILFIYIN*ISTSLG*KGNF*SISNV FGYII*GRDLILFRRIYQLVQYVKPPFPL*LVM LFL*NIKFLCVFCVLGQFLGLLGFFYTVNNYRF NWC*YLVVHRFSLVLLNFVKYNLQIST*NRA*WV KQQLCNHKLDAEEVLPALQNLCVSPLDTHCFPQ S*LS*RMVILPLSF*ELFYQLCV*QYGLHFAHF *LI*K*NNT

Sequence NM_001018092

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA GATATCTTTCTTAGAAGGAGAGGGAAGGAAAGGCCTAAAAGGGAGACTAAAGATGAGTTGCTAAAAAAGAGAGAACAGAAGCAAAGAGACCAGAAGGTTA CCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTGATGACA TCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGA GGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTCAGGGAGATACCGA GAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGTCATCATCTTACATCAGACTTTC TAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAAAGCCCAGTTTGTCTTTCAGAAG GTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATTGTGTATTGGTCTGATATTTTAG TATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTATCAAATAGCATTTATTACATGT CTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATGAAGGGAGCAGTCTAAAGAATTG TATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCT TCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTTTTTTGTTGGTTTGTTTGTTTGG TTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCAATCTAGGTGGAGGTCTTTTTAA ATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCACCTGGCTTGAGTTCCTTGCTGT CACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCAGTACTTTACTTTTTTCCCCCCA AATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTCTCCAGAGAAATTAACTTACATT ATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAATGCTAAGGTGATGTTTTACCGG GACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCTCAAGCAAATGT GAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTTGATGTTATTTCTGGAAGCTGGA TGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACATTTTGATGTTGTGTTTTTGCTTA TTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATATATTGTCAATATGTTCCCATATT AAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTCAGCAATTTGATTATGTATTATA ATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCTCTAAAAATGAAATTGTTAGGTG AAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAATATATCCAATGCTCAGAATTGAAAATTA CTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGTGAAGGTTGTGTTTTTTTTCATA TTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCTCTTTTTTTGGCATATAAGTTCT TAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCAGTATAGAAGTTGTTTTCTTTAG GAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTATTTTCTTATTTCACTTTTACACC TATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTTAATCCATCTCAAATGTATTTGG TTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCCCCTCTGATTAGTAATGCTATTT TTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTCAATAATTATCGCTTTAATTGGT GTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAAACAGAGCTTAATGGGTAAAGCA ACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACACTCACTGTTTCCCCCAGAGCTAA CTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTACATTTTGCTCACTTTTGACTTA TATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K RYLS*KEREGKA*KGD*R*VAKKERTEAKRPEGY LFDKMIHLVIARRVGLLFPQKSGGAGISSILMT SQQLGFYHFTICPRSCSP*KNPWHFRNSRNRIM RRCKQSSQRKN*LKD*ILKCGVIIQKGSLQGDTE K*GMKMTIGPLMNSEDNLLNH*R*DVIILHQTF *LVSRSVSDIVEGSNFIKLHKGSYKKSPVCLSE GDFHVLEKFNI*ILCLTTWY*NFAIYCVLV*YFS I**NILFFSLSQMKRGNFAFFLFLTYQIAFITC LSVKYLVVPGT*NQLGRHSSLLFWVMKGAV*RI VCMFAWGY*DIGCEIPRKTCMENA*QMLFSFFCL PLGRTLSLF*LGRTSLLSRCWKVEVFFVGLFVW FCVFF*DGVLLCRPGWSAVAGSRLTAI*VEVFL NNLMLLQANLVQEKPKLTELGEGLCITWLEFLAV TDDRFMYTTLMFDKESIFFLLKLNCQYFTFFPP NRYI*TAEASVPYINVHVILEDYMVVSREINLH YQKILCLIYWSL*KC*RIKY*LEQLQMLR*CFTG T*LNTTFSFRKRLFTFASRCNYCFHSLPPSSKC EKYTDLRFSLVLVLKTNSK*FHSVKFDVISGSW MIWKSSCAWTHSSL*SCHLGR*TLLTF*CCVFAY FSMHSYAFIFSKMKS*CTLLCSLLYILSICSHI KYFAF*TAVGYFISWVYHNFKIYFLFSNLIMYY NVICK**YD*HLNHIHRK*LISRRYRSKNEIVR* KCF*EFLYILLTAEIMFIYIPISNISNAQN*KL LLKIFAN*IITEKGYI*NLYLFFLLSEGCVFFH IHWPCYIHSFENCSFIFFLLLAWRICSFFGI*VL KNVRPLSYIVTFFSLICHLT*FVVFQYRSCFL* ESTFVYVLESIFVIQEWINVLIFF*YFLISLLH LSLKGI*ILFIYIN*ISTSLG*KGNF*SISNVFG YII*GRDLILFRRIYQLVQYVKPPFPL*LVMLF L*NIKFLCVFCVLGQFLGLLGFFYTVNNYRFNW C*YLVVHRFSLVLLNFVKYNLQIST*NRA*WVKQ QLCNHKLDAEEVLPALQNLCVSPLDTHCFPQS* LS*RMVILPLSF*ELFYQLCV*QYGLHFAHF*L I*K*NNT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGD*IIKEKVATETALNTAAFRK DIFLRRRGKERPKRETKDELLKKREQKQRDQKV TFSTK*FI*SLPEEWVSYFLRRAAAQG*AAS**H HSSSASTTSPYAHAAALHRRILGTSETAETEL* GDASKARSAKIS*KTEY*NAEL*SRRGVFREIP RSKG*R*RLVL**ILKIIS*ITDVEMSSSYIRLS N*YQDQCQILLREVIL*SYTKVVIKKAQFVFQK VTFMCLKSLIFEYCV*PHGIKILQYIVYWSDIL VYSRTYFFFL*AK*KEVTLLFSFFLPIK*HLLHV FQ*NT*LFQAPKIN*EDIVPFFFG**REQSKEL YACLHGVIETLDVKYLGRHVWRMLNKCSSPFSV FP*EERYLSSD*EGLPF*VDAGKWRFFLLVCLFG FVFFFEMGSCSVAQAGVQWQDLGSLQSRWRSF* ITSCYFRQIWYRRSPS*LSWVKACASPGLSSLL SQMIGSCTQH*CLIRKAFFFC*N*IVSTLLFSPQ IGIYRLQKLQSHI*TFMSF*RIIWLSPEKLTYI IKKFFV*YIGVFKSVEE*NIN*NNFKC*GDVLP GLN*IQLFPFEKDCSHLLPGVIIAFIPYPPQANV KSILT*DSH*F*FLKRIVNDFTA*NLMLFLEAG *YGRVHVPGHTVPCNPAI*GDKLC*HFDVVFLL IFLCIVMHLYSQK*NHNVHCFVVCSIYCQYVPIL NILLFRQL*DILFHGYTITLRFISYSAI*LCII M*SVNSDMINILITSIENDLFPEDTALKMKLLG ESVFENFYIYYSLQRSCSFTFPLAIYPMLRIENY YLKSLQIE*LQKRATFKICICFSYLVKVVFFFI FIGHAIFILLRIAHSYFFCCLLGEFALFLAYKF LRMSVLCHIL*LFLALFVI*HSLWYFSIEVVFFR SLPLCMCLKVFL*SKNG*MSLFSSSIFLFHFYT YL*KEFKFCLYTLTKSVQVWGEKEIFNPSQMYL VILYKVGI*FYFEEFTNWFSM*NHHFPSD**CYF YRILNSYVSFVS*ANFWAFWVFSTLSIIIALIG VNIW*YTGSPLFF*IL*NITYK*VLKTELNG*S NSCVITN*MQRRSCQHSKISVCPLLTLTVSPRAN YPDVW*FSLCPFKNYFTSYVYNNMVYILLTFDL YKNRIIL

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK ISFLEGEGRKGLKGRLKMSC*KRENRSKETRRL PFRQNDSSSHCQKSGSPISSEERRRRDKQHLDD ITAARLLPLHHMPTQLLSIEESLALQKQQKQNYE EMQAKLAAQKLAERLNIKMRSYNPEGESSGRYR EVRDEDDDWSSDEF*R*SPKSLTLRCHHLTSDF LTSIKISVRYC*GK*FYKVTQR*L*KKPSLSFRR *LSCA*KV*YLNIVFNHMVLKFCNILCIGLIF* YIVEHTFFFFKPNEKR*LCFFPFSYLSNSIYYM SFSEILSCSRHLKSIRKT*FPSFLGNEGSSLKNC MHVCMGLLRHWM*NT*EDMYGECLTNALLLFLS SLRKNVISLLIRKDFPFE*MLESGGFFCWFVCL VLCFFLRWGLALSPRLECSGRISAHCNLGGGLFK *PHVTSGKSGTGEAQAN*AG*RLVHHLA*VPCC HR**VHVHNINV**GKHFFFVKIELSVLYFFPP K*VYIDCRSFSPIYKRSCHFRGLYGCLQRN*LTL SKNSLSNILESLKVLKNKILIRTTSNAKVMFYR DLIKYNFFLSKKTVHICFQV*LLLSFLTPLKQM *KVY*PKILISFSS*NE**MISQRKI*CYFWKLD DMEEFMCLDTQFPVILPSREINSVNILMLCFCL FFYA*LCIYILKNEIIMYIAL*SALYIVNMFPY *IFCFLDSCRIFYFMGIP*L*DLFPIQQFDYVL* CNL*IVI*LTS*SHP*KMTYFQKIPL*K*NC*V KVFLRIFIYITHCRDHVHLHSH*QYIQCSELKI TT*NLCKLNNYRKGLHLKFVFVFLT**RLCFFSY SLAMLYSFF*ELLIHIFSVACLENLLFFWHISS *ECPSFVIYCNFF*PYLSFNIVCGISV*KLFSL GVYLCVCA*KYFCNPRMDKCPYFLLVFSYFTFTP IFKRNLNFVYIH*LNQYKFGVKRKFLIHLKCIW LYYIR*GFDFISKNLPIGSVCETTISPLISNAI FIEY*ILMCLLCLRPISGPSGFFLHCQ*LSL*LV LIFGSTQVLPCSFKFCEI*LTNKYLKQSLMGKA TAV*SQTRCRGGLASTPKSLCVPS*HSLFPPEL TILTYGNSPSVLLRIILPVMCITIWFTFCSLLTY IKIE*Y

Sequence NM_001018094

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCT CAGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGA AGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATG CGGAGTTATAATCCAGAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTA AATCACTGACGTTGAGATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTAC ACAAAGGTAGTTATAAAAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTA TTAAAATTTTGCAATATATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCT TTTTTCCTTTTTCTTACCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTT CCCTTCTTTTTTGGGTAATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATG TATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGA TGCTGGAAAGTGGAGGTTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGG CAGGATCTCGGCTCACTGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCT GGGTGAAGGCTTGTGCATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTT TTGTTAAAATTGAATTGTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTT TAGAGGATTATATGGTTGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATA TTAATTAGAACAACTTCAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGG TGTAATTATTGCTTTCATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAAT GATTTCACAGCGTAAAATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGG GAGATAAACTCTGTTAACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTG CTTTGTAGTCTGCTCTATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACT TTAAGATTTATTTCCTATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACT TATTTCCAGAAGATACCGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTAC ATTCCCATTAGCAATATATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGT ATTTGTTTTTCTTACTTAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTT GCTTGCTTGGAGAATTTGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTA ACATAGTTTGTGGTATTTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATG TCCTTATTTTCTTCTAGTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTT GGGGTGAAAAGGAAATTTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAG TATGTGAAACCACCATTTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTC TGGGTTTTTTCTACACTGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAA CTTACAAATAAGTACTTAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTC TGTGTGTCCCCTCTTGACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTAT GTGTATAACAATATGGTTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA

refseq_Gcom1.F1 (forward primer)
refseq_Gcom1.R1 (reverse primer)
Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC IASRRKSSRWSRRLPFRQNDSSSHCQKSGSPIS SEERRRRDKQHLDDITAARLLPLHHMPTQLLSIE ESLALQKQQKQNYEEMQAKLAAQKLAERLNIKM RSYNPEGESSGRYREVRDEDDDWSSDEF*R*SP KSLTLRCHHLTSDFLTSIKISVRYC*GK*FYKVT QR*L*KKPSLSFRR*LSCA*KV*YLNIVFNHMV LKFCNILCIGLIF*YIVEHTFFFFKPNEKR*LC FFPFSYLSNSIYYMSFSEILSCSRHLKSIRKT*F PSFLGNEGSSLKNCMHVCMGLLRHWM*NT*EDM YGECLTNALLLFLSSLRKNVISLLIRKDFPFE* MLESGGFFCWFVCLVLCFFLRWGLALSPRLECSG RISAHCNLGGGLFK*PHVTSGKSGTGEAQAN*A G*RLVHHLA*VPCCHR**VHVHNINV**GKHFF FVKIELSVLYFFPPK*VYIDCRSFSPIYKRSCHF RGLYGCLQRN*LTLSKNSLSNILESLKVLKNKI LIRTTSNAKVMFYRDLIKYNFFLSKKTVHICFQ V*LLLSFLTPLKQM*KVY*PKILISFSS*NE**M ISQRKI*CYFWKLDDMEEFMCLDTQFPVILPSR EINSVNILMLCFCLFFYA*LCIYILKNEIIMYI AL*SALYIVNMFPY*IFCFLDSCRIFYFMGIP*L *DLFPIQQFDYVL*CNL*IVI*LTS*SHP*KMT YFQKIPL*K*NC*VKVFLRIFIYITHCRDHVHL HSH*QYIQCSELKITT*NLCKLNNYRKGLHLKFV FVFLT**RLCFFSYSLAMLYSFF*ELLIHIFSV ACLENLLFFWHISS*ECPSFVIYCNFF*PYLSF NIVCGISV*KLFSLGVYLCVCA*KYFCNPRMDKC PYFLLVFSYFTFTPIFKRNLNFVYIH*LNQYKF GVKRKFLIHLKCIWLYYIR*GFDFISKNLPIGS VCETTISPLISNAIFIEY*ILMCLLCLRPISGPS GFFLHCQ*LSL*LVLIFGSTQVLPCSFKFCEI* LTNKYLKQSLMGKATAV*SQTRCRGGLASTPKS LCVPS*HSLFPPELTILTYGNSPSVLLRIILPVM CITIWFTFCSLLTYIKIE*Y

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH QSAERRDELHQRCESHFGKGEKANVWRL**DET EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF* CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA LPAEESQADGRGGYLFDKMIHLVIARRVGLLFP QKSGGAGISSILMTSQQLGFYHFTICPRSCSP* KNPWHFRNSRNRIMRRCKQSSQRKN*LKD*ILKC GVIIQKGSLQGDTEK*GMKMTIGPLMNSEDNLL NH*R*DVIILHQTF*LVSRSVSDIVEGSNFIKL HKGSYKKSPVCLSEGDFHVLEKFNI*ILCLTTWY *NFAIYCVLV*YFSI**NILFFSLSQMKRGNFA FFLFLTYQIAFITCLSVKYLVVPGT*NQLGRHS SLLFWVMKGAV*RIVCMFAWGY*DIGCEIPRKTC MENA*QMLFSFFCLPLGRTLSLF*LGRTSLLSR CWKVEVFFVGLFVWFCVFF*DGVLLCRPGWSAV AGSRLTAI*VEVFLNNLMLLQANLVQEKPKLTEL GEGLCITWLEFLAVTDDRFMYTTLMFDKESIFF LLKLNCQYFTFFPPNRYI*TAEASVPYINVHVI LEDYMVVSREINLHYQKILCLIYWSL*KC*RIKY *LEQLQMLR*CFTGT*LNTTFSFRKRLFTFASR CNYCFHSLPPSSKCEKYTDLRFSLVLVLKTNSK *FHSVKFDVISGSWMIWKSSCAWTHSSL*SCHLG R*TLLTF*CCVFAYFSMHSYAFIFSKMKS*CTL LCSLLYILSICSHIKYFAF*TAVGYFISWVYHN FKIYFLFSNLIMYYNVICK**YD*HLNHIHRK*L ISRRYRSKNEIVR*KCF*EFLYILLTAEIMFIY IPISNISNAQN*KLLLKIFAN*IITEKGYI*NL YLFFLLSEGCVFFHIHWPCYIHSFENCSFIFFLL LAWRICSFFGI*VLKNVRPLSYIVTFFSLICHL T*FVVFQYRSCFL*ESTFVYVLESIFVIQEWIN VLIFF*YFLISLLHLSLKGI*ILFIYIN*ISTSL G*KGNF*SISNVFGYII*GRDLILFRRIYQLVQ YVKPPFPL*LVMLFL*NIKFLCVFCVLGQFLGL LGFFYTVNNYRFNWC*YLVVHRFSLVLLNFVKYN LQIST*NRA*WVKQQLCNHKLDAEEVLPALQNL CVSPLDTHCFPQS*LS*RMVILPLSF*ELFYQL CV*QYGLHFAHF*LI*K*NNT

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE KDQRIGELDRLIERMEKERHQLQLQLLEHETEM SGELTDSDKERYQQLEEASASLRERIRHLDDMV HCQQKKVKQMVEEVTFSTK*FI*SLPEEWVSYFL RRAAAQG*AAS**HHSSSASTTSPYAHAAALHR RILGTSETAETEL*GDASKARSAKIS*KTEY*N AEL*SRRGVFREIPRSKG*R*RLVL**ILKIIS* ITDVEMSSSYIRLSN*YQDQCQILLREVIL*SY TKVVIKKAQFVFQKVTFMCLKSLIFEYCV*PHG IKILQYIVYWSDILVYSRTYFFFL*AK*KEVTLL FSFFLPIK*HLLHVFQ*NT*LFQAPKIN*EDIV PFFFG**REQSKELYACLHGVIETLDVKYLGRH VWRMLNKCSSPFSVFP*EERYLSSD*EGLPF*VD AGKWRFFLLVCLFGFVFFFEMGSCSVAQAGVQW QDLGSLQSRWRSF*ITSCYFRQIWYRRSPS*LS WVKACASPGLSSLLSQMIGSCTQH*CLIRKAFFF C*N*IVSTLLFSPQIGIYRLQKLQSHI*TFMSF *RIIWLSPEKLTYIIKKFFV*YIGVFKSVEE*N IN*NNFKC*GDVLPGLN*IQLFPFEKDCSHLLPG VIIAFIPYPPQANVKSILT*DSH*F*FLKRIVN DFTA*NLMLFLEAG*YGRVHVPGHTVPCNPAI* GDKLC*HFDVVFLLIFLCIVMHLYSQK*NHNVHC FVVCSIYCQYVPILNILLFRQL*DILFHGYTIT LRFISYSAI*LCIIM*SVNSDMINILITSIEND LFPEDTALKMKLLGESVFENFYIYYSLQRSCSFT FPLAIYPMLRIENYYLKSLQIE*LQKRATFKIC ICFSYLVKVVFFFIFIGHAIFILLRIAHSYFFC CLLGEFALFLAYKFLRMSVLCHIL*LFLALFVI* HSLWYFSIEVVFFRSLPLCMCLKVFL*SKNG*M SLFSSSIFLFHFYTYL*KEFKFCLYTLTKSVQV WGEKEIFNPSQMYLVILYKVGI*FYFEEFTNWFS M*NHHFPSD**CYFYRILNSYVSFVS*ANFWAF WVFSTLSIIIALIGVNIW*YTGSPLFF*IL*NI TYK*VLKTELNG*SNSCVITN*MQRRSCQHSKIS VCPLLTLTVSPRANYPDVW*FSLCPFKNYFTSY VYNNMVYILLTFDLYKNRIIL