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Sequences

Sequence NM_001018095

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGAT
CGTTTTAATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAA
TCAGAAATCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAAT
GAAGGTGGAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGG
AAGCACAGTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCC
TAGAGGAGAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAAC
AGAAATGTCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGAC
ATGGTGCATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTG
AAGTGGAAACCA
GAGAGATAGGAGTGGGCTGTGATCTTCTACCCAGGCAAAGTAGAAAATTCGAAAAGGTCCTCAACGAGTTTGTACAGCTGCTCCCACT
CCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATTTTGGATTGTTGCAATGACTTGGGAGTGCCATAGCACCTAGCAGGCAGAAG
CCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAAGTCCTTCTCCAAATGCATTGAGCCCCAAGAAAAATCGCTGATTCTGAGGA
ACCTTCCAACTTTAACAATTTCAGGACGTCCCTAAGCTGAGCTAGGAGGCACCACGCACAGGCCGGAGGCACCACGCACAGGCCAGAGTCTCCCAAATCC
TGTCCCGCACTCTCTCCCACCTTATTAATGGCCAGCTCATCTTTCAAGTTTCGACTTGAACCACTTCTCAGAGATATCTTCCCAGTGCTTGGTGAGGTCA
CGCTGGCGCTGAATACTTTTTGTATAAACCGACGAACAGCTGATGACCGCATGAATGCTCACACAAGGGGAAAGGGCGCCAAGCCAAGGAGCCGGCGTCA
TCCGACGTAACTTCCGCCCTTTAACGCTCCTGCGGACGCCCCCTTCTCCCAGTCCGTGTTTGTTTCCGGAATTTCAATAAAGCTCGATTCGGCTCGAAGA
AGACCCCGTTCTTCCGGGAAAATGGCGACTCCCGCTCGTGCCCCGGAGTCACCGCCGTCCGCGGATCCGGCGCTAGTAGCGGGGCCTGCCGAGGAAGCCG
AGTGCCCGCCGCCGCGCCAGCCTCAGCCCGCGCAGAATGTGCTCGCTGCCCCGCGGCTTCGAGCCCCAAGCTCCCGAGGACTTGGCGCAGCGGAGTTTGG
TGGAGCTGCGGGAAATGTTGAAGCGCCAGGAGAGACTTTTGCGCAACGA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKMFPMLSRSIWRITSKPSRLPWI
VLMP*TQPWHQIPLACKKPSWM*LWKTATLRIK
SEICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K
*RWNRPKKPMLR*CER*PRSCTASMRRSCRKNRG
STVLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA
*RRKTRGSGSWTG*LSAWKRNVINCNFNS*NMK
QKCLGS*LILTRKGISSWRRHQPASVSGSDT*MT
WCIASRRKSSRWSRRIMNYKAGWTI*QKPRPRL
KWKP
ER*EWAVIFYPGKVENSKRSSTSLYSCSH
SLITSCGPLEMYVGGDILDCCNDLGVP*HLAGRS
QKHQMS*QCLRRCYK*SPSPNALSPKKNR*F*G
TFQL*QFQDVPKLS*EAPRTGRRHHAQARVSQI
LSRTLSHLINGQLIFQVST*TTSQRYLPSAW*GH
AGAEYFLYKPTNS**PHECSHKGKGRQAKEPAS
SDVTSAL*RSCGRPLLPVRVCFRNFNKARFGSK
KTPFFRENGDSRSCPGVTAVRGSGASSGACRGSR
VPAAAPASARAECARCPAASSPKLPRTWRSGVW
WSCGKC*SARRDFCAT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCFPCSAGVSGESHPNPVVCPG
SF*CHELSLGIRFHWPAKNPRGCDFGKQQH*GSN
QKSAADV*SIHGQAEGKAEAVGGSAS*KPAAKN
EGGIVPRSQC*GDARDDQEAVQPV*GEAAGRTE
EAQC*EGGSFGRNQ*FSESD*RSQ*KDASSRDQP
RGERPEDRGAGQAD*AHGKGTSSTATSTPRT*N
RNVWGVN*F*QGKVSAVGGGISQPP*ADQTPR*
HGALPAEESQADGRGE**TTKQVGLFNRNPGQD*
SGNQ
RDRSGL*SSTQAK*KIRKGPQRVCTAAPT
PSSPLVGLWKCMLEETFWIVAMTWECHST*QAE
ARNTKCLNNA*GGATNEVLLQMH*APRKIADSEE
PSNFNNFRTSLS*ARRHHAQAGGTTHRPESPKS
CPALSPTLLMASSSFKFRLEPLLRDIFPVLGEV
TLALNTFCINRRTADDRMNAHTRGKGAKPRSRRH
PT*LPPFNAPADAPFSQSVFVSGISIKLDSARR
RPRSSGKMATPARAPESPPSADPALVAGPAEEA
ECPPPRQPQPAQNVLAAPRLRAPSSRGLGAAEFG
GAAGNVEAPGETFAQR

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVSHAQQEYLENHIQTQSSALD
RFNAMNSALASDSIGLQKTLVDVTLENSNIKDQ
IRNLQQTYEASMDKLREKQRQLEVAQVENQLLKM
KVESSQEANAEVMREMTKKLYSQYEEKLQEEQR
KHSAEKEALLEETNSFLKAIEEANKKMQAAEIS
LEEKDQRIGELDRLIERMEKERHQLQLQLLEHET
EMSGELTDSDKERYQQLEEASASLRERIRHLDD
MVHCQQKKVKQMVEENNELQSRLDYLTETQAKT
EVET
REIGVGCDLLPRQSRKFEKVLNEFVQLLPL
PHHLLWAFGNVCWRRHFGLLQ*LGSAIAPSRQK
PETPNVLTMLEAVLQMKSFSKCIEPQEKSLILR
NLPTLTISGRP*AELGGTTHRPEAPRTGQSLPNP
VPHSLPPY*WPAHLSSFDLNHFSEISSQCLVRS
RWR*ILFV*TDEQLMTA*MLTQGERAPSQGAGV
IRRNFRPLTLLRTPPSPSPCLFPEFQ*SSIRLEE
DPVLPGKWRLPLVPRSHRRPRIRR**RGLPRKP
SARRRASLSPRRMCSLPRGFEPQAPEDLAQRSL
VELREMLKRQERLLRN

Sequence NM_001018101

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGA
AACCA
GAGAGATAGGAGTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGA
GTCCTGGAGTTAACCATGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCC
TTTGGGAAGGGTGACTGTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGG
CAGAGTGAGCAGGTAAGAGAGGGATATACAAGGTCACATTTCAGACACCCACTCGGCATACCCTGCCGTACTGCATCATCATTTGTTTTCTTTGTAGACA
CTGAAATCCTATCAGGAGGATTCCTTCACAATGTATTTTATTTGCTAGACTTTGGTTGGGAGGGAAAAGGACATTAATTTGAAGTTTCATGTTATTCATG
CCAGGATTGTTTGATAGAGCATGAAGGTTTTGTTTACCCATAAAAGTATTAGAGGCAGCGTTTCTCTGATACAGAGAGGCCTGTCCACAAGAAGCATGGG
CACCCAGCCAAACTTGAACCTGGAAGGGAGGGTTCCCGGCCTGCAGGTGCTCTTTCCTCTTGGTCCCAAGCATCTGTGCAGGGTCGTGGGAGCCACACTG
AGAGACTTGTGTGGGCCAGACAAGCTTCATTCTGATGCGCTAGTCCCTTGGTTTAATTTGTGCCTTATGCTTTCATTGGACCAGCTGAAATCACTGTATT
TATTCAACTAGTGATTTTTTTTTCTTTCTCACTTTAACTTAAAGAGAATTTTATATGTCTTGGAAATTTAATAATTTAGTGTTCTCAGTATCAATTGGTG
TTTTTGTTAAACGAATGAATCATCTGTTCATGCATGCTCTACTTTGATATTATAACCTATGTCACATGTGTTTAATAAATACCATATATTTTGTTCTACT
AA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRIMNYKAGWTI*QKPRPRLKW
KP
ER*EWAVIFYPAKQAGLVKL*CLLGTTPHTQE
SWS*P*RKL*LRHSEAYTFYRWTKALEPCGFKS
FGKGDCCFPYTQCKPEWESLRL*STSKTGRKVK
AE*AGKRGIYKVTFQTPTRHTLPYCIIICFLCRH
*NPIRRIPSQCILFARLWLGGKRTLI*SFMLFM
PGLFDRA*RFCLPIKVLEAAFL*YREACPQEAW
APSQT*TWKGGFPACRCSFLLVPSICAGSWEPH*
ETCVGQTSFILMR*SLGLICALCFHWTS*NHCI
YSTSDFFFFLTLT*REFYMSWKFNNLVFSVSIG
VFVKRMNHLFMHALL*YYNLCHMCLINTIYFVLL

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGE**TTKQVGLFNRNPGQD*SG
NQ
RDRSGL*SSTQPNRQDS*NCDAF*ELHPIHK
SPGVNHEENSDLGTQRHTLFTDGQKLWNPVASNP
LGRVTVVSPTHSVSRNGNR*GSDPLLRQEGK*R
QSEQVREGYTRSHFRHPLGIPCRTASSFVFFVD
TEILSGGFLHNVFYLLDFGWEGKGH*FEVSCYSC
QDCLIEHEGFVYP*KY*RQRFSDTERPVHKKHG
HPAKLEPGREGSRPAGALSSWSQASVQGRGSHT
ERLVWARQASF*CASPLV*FVPYAFIGPAEITVF
IQLVIFFSFSL*LKENFICLGNLII*CSQYQLV
FLLNE*IICSCMLYFDIITYVTCV**IPYILFY
*

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEENNELQSRLDYLTETQAKTEVE
T
REIGVGCDLLPSQTGRTREIVMPSRNYTPYTR
VLELTMKKTLT*ALRGIHFLQMDKSSGTLWLQI
LWEG*LLFPLHTV*AGMGIAEALIHF*DRKESEG
RVSR*ERDIQGHISDTHSAYPAVLHHHLFSL*T
LKSYQEDSFTMYFIC*TLVGREKDINLKFHVIH
ARIV**SMKVLFTHKSIRGSVSLIQRGLSTRSMG
TQPNLNLEGRVPGLQVLFPLGPKHLCRVVGATL
RDLCGPDKLHSDALVPWFNLCLMLSLDQLKSLY
LFN**FFFLSHFNLKRILYVLEI**FSVLSINWC
FC*TNESSVHACSTLIL*PMSHVFNKYHIFCST

Sequence NM_001018097

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGA
AACCA
GAGAGATAGGAGTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGA
GTCCTGGAGTTAACCATGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCC
TTTGGGAAGGGTGACTGTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGG
CAGAGTGAGCAGGTAAATACCTAGCAGAAGAATGGCTGGGTCAAACGGTATATTTTAAGAAACTGCCAAACTGGTTTCCAAAGAGGTTGTACTATTTTGC
ATTCCCATTAGCAGTGTATCAAAGTTCCAGTTGCTCTACATCCTTTACAACACTTGGAATGGTCAGTCTTTCTGGCAAAGTAGAAAATTCGAAAAGGTCC
TCAACGAGTTTGTACAGCTGCTCCCACTCCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATTTTGGATTGTTGCAATGACTTGG
GAGTGCCATAGCACCTAGCAGGCAGAAGCCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAAGTCCTTCTCCAAATGCATTGAG
CCCCAAGAAAAATCGCTGATTCTGAGGAACCTTCCAACTTTAACAATTTCAGGACGTCCCTAAGCTGAGCTAGGAGGCACCACGCACAGGCCGGAGGCAC
CACGCACAGGCCAGAGTCTCCCAAATCCTGTCCCGCACTCTCTCCCACCTTATTAATGGCCAGCTCATCTTTCAAGTTTCGACTTGAACCACTTCTCAGA
GATATCTTCCCAGAAAATTCATTTGCAAATTGCCCGACAAAGGTAAAAAGATCTTTGACTCTTTTGCCAAACTGAAAGCTGCCATTGCAGAATGTGAAGA
AGTTAGAAGAAAAAGTGAACTGTTTAACCCTGTTAGTTTAGACTGTAAGCTAAGGCAAAAAGCAATTGCAGAAGTTGATGTGGGTACAGATAAGGCCCAG
AATTCTGACCCGATACTTGATACTTCATCACTAGTTCCTGGATGTTCCTCTGTAGATAACATCAAGTCATCTCAAACCTCACAAAACCAGGGACTTGGAC
GTCCTACTCTTGAAGGTGATGAAGAGACTTCAGAGGTTGAGTACACAGTGAATAAGGGCCCAGCTTCCAGCAATAGAGACAGGGTACCACCTTCATCTGA
AGCTAGTGAGCATCACCCGCGGCATCGTGTTTCAAGTCAAGCGGAAGATACTTCCAGCAGCTTTGACAACCTGTTTATTGACAGGTTACAGAGGATCACC
ATTGCGGACCAAGGTGAACAACAGTCAGAAGAAAACGCAAGTACTAAGAACTTGACAGGCCTTTCCAGTGGGACTGAGAAGAAACCTCATTACATGGAAG
TGCTAGAAATGCGAGCCAAAAACCCAGTGCCCCAGCTGCGTAAATTTAAAACCAATGTGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAA
GAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCC
ACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTG
AAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGA
ATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGG
AAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATA
TTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAA
TGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAA
TCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGT
GAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAA
GGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCG
CCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAG
AAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTG
ATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATA
TATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAA
AAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGAC
TGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGT
TCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCC
CTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAA
TGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTAT
TTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAAT
CACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTG
CAGAGATCATGTTCATTTACATTCCCATTAGCAATATATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAA
GGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGC
TCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTT
TTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGT
AATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATT
AACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAA
GAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTT
AGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTT
TTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCT
TGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTA
AGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRIMNYKAGWTI*QKPRPRLKW
KP
ER*EWAVIFYPAKQAGLVKL*CLLGTTPHTQE
SWS*P*RKL*LRHSEAYTFYRWTKALEPCGFKS
FGKGDCCFPYTQCKPEWESLRL*STSKTGRKVK
AE*AGKYLAEEWLGQTVYFKKLPNWFPKRLYYFA
FPLAVYQSSSCSTSFTTLGMVSLSGKVENSKRS
STSLYSCSHSLITSCGPLEMYVGGDILDCCNDL
GVP*HLAGRSQKHQMS*QCLRRCYK*SPSPNALS
PKKNR*F*GTFQL*QFQDVPKLS*EAPRTGRRH
HAQARVSQILSRTLSHLINGQLIFQVST*TTSQ
RYLPRKFICKLPDKGKKIFDSFAKLKAAIAECEE
VRRKSELFNPVSLDCKLRQKAIAEVDVGTDKAQ
NSDPILDTSSLVPGCSSVDNIKSSQTSQNQGLG
RPTLEGDEETSEVEYTVNKGPASSNRDRVPPSSE
ASEHHPRHRVSSQAEDTSSSFDNLFIDRLQRIT
IADQGEQQSEENASTKNLTGLSSGTEKKPHYME
VLEMRAKNPVPQLRKFKTNVLPFRQNDSSSHCQK
SGSPISSEERRRRDKQHLDDITAARLLPLHHMP
TQLLSIEESLALQKQQKQNYEEMQAKLAAQKLA
ERLNIKMRSYNPEGESSGRYREVRDEDDDWSSDE
F*R*SPKSLTLRCHHLTSDFLTSIKISVRYC*G
K*FYKVTQR*L*KKPSLSFRR*LSCA*KV*YLN
IVFNHMVLKFCNILCIGLIF*YIVEHTFFFFKPN
EKR*LCFFPFSYLSNSIYYMSFSEILSCSRHLK
SIRKT*FPSFLGNEGSSLKNCMHVCMGLLRHWM
*NT*EDMYGECLTNALLLFLSSLRKNVISLLIRK
DFPFE*MLESGGFFCWFVCLVLCFFLRWGLALS
PRLECSGRISAHCNLGGGLFK*PHVTSGKSGTG
EAQAN*AG*RLVHHLA*VPCCHR**VHVHNINV*
*GKHFFFVKIELSVLYFFPPK*VYIDCRSFSPI
YKRSCHFRGLYGCLQRN*LTLSKNSLSNILESL
KVLKNKILIRTTSNAKVMFYRDLIKYNFFLSKKT
VHICFQV*LLLSFLTPLKQM*KVY*PKILISFS
S*NE**MISQRKI*CYFWKLDDMEEFMCLDTQF
PVILPSREINSVNILMLCFCLFFYA*LCIYILKN
EIIMYIAL*SALYIVNMFPY*IFCFLDSCRIFY
FMGIP*L*DLFPIQQFDYVL*CNL*IVI*LTS*
SHP*KMTYFQKIPL*K*NC*VKVFLRIFIYITHC
RDHVHLHSH*QYIQCSELKITT*NLCKLNNYRK
GLHLKFVFVFLT**RLCFFSYSLAMLYSFF*EL
LIHIFSVACLENLLFFWHISS*ECPSFVIYCNFF
*PYLSFNIVCGISV*KLFSLGVYLCVCA*KYFC
NPRMDKCPYFLLVFSYFTFTPIFKRNLNFVYIH
*LNQYKFGVKRKFLIHLKCIWLYYIR*GFDFISK
NLPIGSVCETTISPLISNAIFIEY*ILMCLLCL
RPISGPSGFFLHCQ*LSL*LVLIFGSTQVLPCS
FKFCEI*LTNKYLKQSLMGKATAV*SQTRCRGGL
ASTPKSLCVPS*HSLFPPELTILTYGNSPSVLL
RIILPVMCITIWFTFCSLLTYIKIE*Y

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGE**TTKQVGLFNRNPGQD*SG
NQ
RDRSGL*SSTQPNRQDS*NCDAF*ELHPIHK
SPGVNHEENSDLGTQRHTLFTDGQKLWNPVASNP
LGRVTVVSPTHSVSRNGNR*GSDPLLRQEGK*R
QSEQVNT*QKNGWVKRYILRNCQTGFQRGCTIL
HSH*QCIKVPVALHPLQHLEWSVFLAK*KIRKGP
QRVCTAAPTPSSPLVGLWKCMLEETFWIVAMTW
ECHST*QAEARNTKCLNNA*GGATNEVLLQMH*
APRKIADSEEPSNFNNFRTSLS*ARRHHAQAGGT
THRPESPKSCPALSPTLLMASSSFKFRLEPLLR
DIFPENSFANCPTKVKRSLTLLPN*KLPLQNVK
KLEEKVNCLTLLV*TVS*GKKQLQKLMWVQIRPR
ILTRYLILHH*FLDVPL*ITSSHLKPHKTRDLD
VLLLKVMKRLQRLSTQ*IRAQLPAIETGYHLHL
KLVSITRGIVFQVKRKILPAALTTCLLTGYRGSP
LRTKVNNSQKKTQVLRT*QAFPVGLRRNLITWK
C*KCEPKTQCPSCVNLKPMCYLFDKMIHLVIAR
RVGLLFPQKSGGAGISSILMTSQQLGFYHFTICP
RSCSP*KNPWHFRNSRNRIMRRCKQSSQRKN*L
KD*ILKCGVIIQKGSLQGDTEK*GMKMTIGPLM
NSEDNLLNH*R*DVIILHQTF*LVSRSVSDIVEG
SNFIKLHKGSYKKSPVCLSEGDFHVLEKFNI*I
LCLTTWY*NFAIYCVLV*YFSI**NILFFSLSQ
MKRGNFAFFLFLTYQIAFITCLSVKYLVVPGT*N
QLGRHSSLLFWVMKGAV*RIVCMFAWGY*DIGC
EIPRKTCMENA*QMLFSFFCLPLGRTLSLF*LG
RTSLLSRCWKVEVFFVGLFVWFCVFF*DGVLLCR
PGWSAVAGSRLTAI*VEVFLNNLMLLQANLVQE
KPKLTELGEGLCITWLEFLAVTDDRFMYTTLMF
DKESIFFLLKLNCQYFTFFPPNRYI*TAEASVPY
INVHVILEDYMVVSREINLHYQKILCLIYWSL*
KC*RIKY*LEQLQMLR*CFTGT*LNTTFSFRKR
LFTFASRCNYCFHSLPPSSKCEKYTDLRFSLVLV
LKTNSK*FHSVKFDVISGSWMIWKSSCAWTHSS
L*SCHLGR*TLLTF*CCVFAYFSMHSYAFIFSK
MKS*CTLLCSLLYILSICSHIKYFAF*TAVGYFI
SWVYHNFKIYFLFSNLIMYYNVICK**YD*HLN
HIHRK*LISRRYRSKNEIVR*KCF*EFLYILLT
AEIMFIYIPISNISNAQN*KLLLKIFAN*IITEK
GYI*NLYLFFLLSEGCVFFHIHWPCYIHSFENC
SFIFFLLLAWRICSFFGI*VLKNVRPLSYIVTF
FSLICHLT*FVVFQYRSCFL*ESTFVYVLESIFV
IQEWINVLIFF*YFLISLLHLSLKGI*ILFIYI
N*ISTSLG*KGNF*SISNVFGYII*GRDLILFR
RIYQLVQYVKPPFPL*LVMLFL*NIKFLCVFCVL
GQFLGLLGFFYTVNNYRFNWC*YLVVHRFSLVL
LNFVKYNLQIST*NRA*WVKQQLCNHKLDAEEV
LPALQNLCVSPLDTHCFPQS*LS*RMVILPLSF*
ELFYQLCV*QYGLHFAHF*LI*K*NNT

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEENNELQSRLDYLTETQAKTEVE
T
REIGVGCDLLPSQTGRTREIVMPSRNYTPYTR
VLELTMKKTLT*ALRGIHFLQMDKSSGTLWLQI
LWEG*LLFPLHTV*AGMGIAEALIHF*DRKESEG
RVSR*IPSRRMAGSNGIF*ETAKLVSKEVVLFC
IPISSVSKFQLLYILYNTWNGQSFWQSRKFEKV
LNEFVQLLPLPHHLLWAFGNVCWRRHFGLLQ*LG
SAIAPSRQKPETPNVLTMLEAVLQMKSFSKCIE
PQEKSLILRNLPTLTISGRP*AELGGTTHRPEA
PRTGQSLPNPVPHSLPPY*WPAHLSSFDLNHFSE
ISSQKIHLQIARQR*KDL*LFCQTESCHCRM*R
S*KKK*TV*PC*FRL*AKAKSNCRS*CGYR*GP
EF*PDT*YFITSSWMFLCR*HQVISNLTKPGTWT
SYS*R**RDFRG*VHSE*GPSFQQ*RQGTTFI*
S**ASPAASCFKSSGRYFQQL*QPVY*QVTEDH
HCGPR*TTVRRKRKY*ELDRPFQWD*EETSLHGS
ARNASQKPSAPAA*I*NQCVTFSTK*FI*SLPE
EWVSYFLRRAAAQG*AAS**HHSSSASTTSPYA
HAAALHRRILGTSETAETEL*GDASKARSAKIS*
KTEY*NAEL*SRRGVFREIPRSKG*R*RLVL**
ILKIIS*ITDVEMSSSYIRLSN*YQDQCQILLR
EVIL*SYTKVVIKKAQFVFQKVTFMCLKSLIFEY
CV*PHGIKILQYIVYWSDILVYSRTYFFFL*AK
*KEVTLLFSFFLPIK*HLLHVFQ*NT*LFQAPK
IN*EDIVPFFFG**REQSKELYACLHGVIETLDV
KYLGRHVWRMLNKCSSPFSVFP*EERYLSSD*E
GLPF*VDAGKWRFFLLVCLFGFVFFFEMGSCSV
AQAGVQWQDLGSLQSRWRSF*ITSCYFRQIWYRR
SPS*LSWVKACASPGLSSLLSQMIGSCTQH*CL
IRKAFFFC*N*IVSTLLFSPQIGIYRLQKLQSH
I*TFMSF*RIIWLSPEKLTYIIKKFFV*YIGVFK
SVEE*NIN*NNFKC*GDVLPGLN*IQLFPFEKD
CSHLLPGVIIAFIPYPPQANVKSILT*DSH*F*
FLKRIVNDFTA*NLMLFLEAG*YGRVHVPGHTVP
CNPAI*GDKLC*HFDVVFLLIFLCIVMHLYSQK
*NHNVHCFVVCSIYCQYVPILNILLFRQL*DIL
FHGYTITLRFISYSAI*LCIIM*SVNSDMINILI
TSIENDLFPEDTALKMKLLGESVFENFYIYYSL
QRSCSFTFPLAIYPMLRIENYYLKSLQIE*LQK
RATFKICICFSYLVKVVFFFIFIGHAIFILLRIA
HSYFFCCLLGEFALFLAYKFLRMSVLCHIL*LF
LALFVI*HSLWYFSIEVVFFRSLPLCMCLKVFL
*SKNG*MSLFSSSIFLFHFYTYL*KEFKFCLYTL
TKSVQVWGEKEIFNPSQMYLVILYKVGI*FYFE
EFTNWFSM*NHHFPSD**CYFYRILNSYVSFVS
*ANFWAFWVFSTLSIIIALIGVNIW*YTGSPLFF
*IL*NITYK*VLKTELNG*SNSCVITN*MQRRS
CQHSKISVCPLLTLTVSPRANYPDVW*FSLCPF
KNYFTSYVYNNMVYILLTFDLYKNRIIL

Sequence NM_001018091

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA
GATATCTTTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA
GTGGGCTGTGATCTTCTACCCAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGG
GGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGAGATGT
CATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATAAAAAA
AGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAATATATT
GTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTTACCTA
TCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGGTAATG
AAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTAACAAA
TGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAGGTTTT
TTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACTGCA
ATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTGCATCA
CCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAATTGTCA
GTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGGTTGTC
TCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACTTCAAA
TGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTTCATTC
CTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAAAATTT
GATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTTAACAT
TTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTCTATAT
ATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCCTATTC
AGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATACCGCT
CTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAATATATC
CAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTACTTAGT
GAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAATTTGCT
CTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTATTTCA
GTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCTAGTAT
TTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAATTTTT
AATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCATTTCC
CCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACACTGTC
AATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTACTTAAA
ACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTTGACAC
TCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATGGTTTA
CATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K
RYLS*KER
IMNYKAGWTI*QKPRPRLKWKPER*E
WAVIFYPGDASKARSAKIS*KTEY*NAEL*SRR
GVFREIPRSKG*R*RLVL**ILKIIS*ITDVEM
SSSYIRLSN*YQDQCQILLREVIL*SYTKVVIKK
AQFVFQKVTFMCLKSLIFEYCV*PHGIKILQYI
VYWSDILVYSRTYFFFL*AK*KEVTLLFSFFLP
IK*HLLHVFQ*NT*LFQAPKIN*EDIVPFFFG**
REQSKELYACLHGVIETLDVKYLGRHVWRMLNK
CSSPFSVFP*EERYLSSD*EGLPF*VDAGKWRF
FLLVCLFGFVFFFEMGSCSVAQAGVQWQDLGSLQ
SRWRSF*ITSCYFRQIWYRRSPS*LSWVKACAS
PGLSSLLSQMIGSCTQH*CLIRKAFFFC*N*IV
STLLFSPQIGIYRLQKLQSHI*TFMSF*RIIWLS
PEKLTYIIKKFFV*YIGVFKSVEE*NIN*NNFK
C*GDVLPGLN*IQLFPFEKDCSHLLPGVIIAFI
PYPPQANVKSILT*DSH*F*FLKRIVNDFTA*NL
MLFLEAG*YGRVHVPGHTVPCNPAI*GDKLC*H
FDVVFLLIFLCIVMHLYSQK*NHNVHCFVVCSI
YCQYVPILNILLFRQL*DILFHGYTITLRFISYS
AI*LCIIM*SVNSDMINILITSIENDLFPEDTA
LKMKLLGESVFENFYIYYSLQRSCSFTFPLAIY
PMLRIENYYLKSLQIE*LQKRATFKICICFSYLV
KVVFFFIFIGHAIFILLRIAHSYFFCCLLGEFA
LFLAYKFLRMSVLCHIL*LFLALFVI*HSLWYF
SIEVVFFRSLPLCMCLKVFL*SKNG*MSLFSSSI
FLFHFYTYL*KEFKFCLYTLTKSVQVWGEKEIF
NPSQMYLVILYKVGI*FYFEEFTNWFSM*NHHF
PSD**CYFYRILNSYVSFVS*ANFWAFWVFSTLS
IIIALIGVNIW*YTGSPLFF*IL*NITYK*VLK
TELNG*SNSCVITN*MQRRSCQHSKISVCPLLT
LTVSPRANYPDVW*FSLCPFKNYFTSYVYNNMVY
ILLTFDLYKNRIIL

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGD*IIKEKVATETALNTAAFRK
DIFLRRR
E**TTKQVGLFNRNPGQD*SGNQRDR
SGL*SSTQEMQAKLAAQKLAERLNIKMRSYNPEG
ESSGRYREVRDEDDDWSSDEF*R*SPKSLTLRC
HHLTSDFLTSIKISVRYC*GK*FYKVTQR*L*K
KPSLSFRR*LSCA*KV*YLNIVFNHMVLKFCNIL
CIGLIF*YIVEHTFFFFKPNEKR*LCFFPFSYL
SNSIYYMSFSEILSCSRHLKSIRKT*FPSFLGN
EGSSLKNCMHVCMGLLRHWM*NT*EDMYGECLTN
ALLLFLSSLRKNVISLLIRKDFPFE*MLESGGF
FCWFVCLVLCFFLRWGLALSPRLECSGRISAHC
NLGGGLFK*PHVTSGKSGTGEAQAN*AG*RLVHH
LA*VPCCHR**VHVHNINV**GKHFFFVKIELS
VLYFFPPK*VYIDCRSFSPIYKRSCHFRGLYGC
LQRN*LTLSKNSLSNILESLKVLKNKILIRTTSN
AKVMFYRDLIKYNFFLSKKTVHICFQV*LLLSF
LTPLKQM*KVY*PKILISFSS*NE**MISQRKI
*CYFWKLDDMEEFMCLDTQFPVILPSREINSVNI
LMLCFCLFFYA*LCIYILKNEIIMYIAL*SALY
IVNMFPY*IFCFLDSCRIFYFMGIP*L*DLFPI
QQFDYVL*CNL*IVI*LTS*SHP*KMTYFQKIPL
*K*NC*VKVFLRIFIYITHCRDHVHLHSH*QYI
QCSELKITT*NLCKLNNYRKGLHLKFVFVFLT*
*RLCFFSYSLAMLYSFF*ELLIHIFSVACLENLL
FFWHISS*ECPSFVIYCNFF*PYLSFNIVCGIS
V*KLFSLGVYLCVCA*KYFCNPRMDKCPYFLLV
FSYFTFTPIFKRNLNFVYIH*LNQYKFGVKRKFL
IHLKCIWLYYIR*GFDFISKNLPIGSVCETTIS
PLISNAIFIEY*ILMCLLCLRPISGPSGFFLHC
Q*LSL*LVLIFGSTQVLPCSFKFCEI*LTNKYLK
QSLMGKATAV*SQTRCRGGLASTPKSLCVPS*H
SLFPPELTILTYGNSPSVLLRIILPVMCITIWF
TFCSLLTYIKIE*Y

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK
ISFLEGE
NNELQSRLDYLTETQAKTEVETREIG
VGCDLLPRRCKQSSQRKN*LKD*ILKCGVIIQK
GSLQGDTEK*GMKMTIGPLMNSEDNLLNH*R*DV
IILHQTF*LVSRSVSDIVEGSNFIKLHKGSYKK
SPVCLSEGDFHVLEKFNI*ILCLTTWY*NFAIY
CVLV*YFSI**NILFFSLSQMKRGNFAFFLFLTY
QIAFITCLSVKYLVVPGT*NQLGRHSSLLFWVM
KGAV*RIVCMFAWGY*DIGCEIPRKTCMENA*Q
MLFSFFCLPLGRTLSLF*LGRTSLLSRCWKVEVF
FVGLFVWFCVFF*DGVLLCRPGWSAVAGSRLTA
I*VEVFLNNLMLLQANLVQEKPKLTELGEGLCI
TWLEFLAVTDDRFMYTTLMFDKESIFFLLKLNCQ
YFTFFPPNRYI*TAEASVPYINVHVILEDYMVV
SREINLHYQKILCLIYWSL*KC*RIKY*LEQLQ
MLR*CFTGT*LNTTFSFRKRLFTFASRCNYCFHS
LPPSSKCEKYTDLRFSLVLVLKTNSK*FHSVKF
DVISGSWMIWKSSCAWTHSSL*SCHLGR*TLLT
F*CCVFAYFSMHSYAFIFSKMKS*CTLLCSLLYI
LSICSHIKYFAF*TAVGYFISWVYHNFKIYFLF
SNLIMYYNVICK**YD*HLNHIHRK*LISRRYR
SKNEIVR*KCF*EFLYILLTAEIMFIYIPISNIS
NAQN*KLLLKIFAN*IITEKGYI*NLYLFFLLS
EGCVFFHIHWPCYIHSFENCSFIFFLLLAWRIC
SFFGI*VLKNVRPLSYIVTFFSLICHLT*FVVFQ
YRSCFL*ESTFVYVLESIFVIQEWINVLIFF*Y
FLISLLHLSLKGI*ILFIYIN*ISTSLG*KGNF
*SISNVFGYII*GRDLILFRRIYQLVQYVKPPFP
L*LVMLFL*NIKFLCVFCVLGQFLGLLGFFYTV
NNYRFNWC*YLVVHRFSLVLLNFVKYNLQIST*
NRA*WVKQQLCNHKLDAEEVLPALQNLCVSPLDT
HCFPQS*LS*RMVILPLSF*ELFYQLCV*QYGL
HFAHF*LI*K*NNT

Sequence NM_001018100

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA
GATATCTTTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA
GTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGAGTCCTGGAGTTAACCA
TGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCCTTTGGGAAGGGTGACT
GTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGGCAGAGTGAGCAGGTAA
GAGAGGGATATACAAGGTCACATTTCAGACACCCACTCGGCATACCCTGCCGTACTGCATCATCATTTGTTTTCTTTGTAGACACTGAAATCCTATCAGG
AGGATTCCTTCACAATGTATTTTATTTGCTAGACTTTGGTTGGGAGGGAAAAGGACATTAATTTGAAGTTTCATGTTATTCATGCCAGGATTGTTTGATA
GAGCATGAAGGTTTTGTTTACCCATAAAAGTATTAGAGGCAGCGTTTCTCTGATACAGAGAGGCCTGTCCACAAGAAGCATGGGCACCCAGCCAAACTTG
AACCTGGAAGGGAGGGTTCCCGGCCTGCAGGTGCTCTTTCCTCTTGGTCCCAAGCATCTGTGCAGGGTCGTGGGAGCCACACTGAGAGACTTGTGTGGGC
CAGACAAGCTTCATTCTGATGCGCTAGTCCCTTGGTTTAATTTGTGCCTTATGCTTTCATTGGACCAGCTGAAATCACTGTATTTATTCAACTAGTGATT
TTTTTTTCTTTCTCACTTTAACTTAAAGAGAATTTTATATGTCTTGGAAATTTAATAATTTAGTGTTCTCAGTATCAATTGGTGTTTTTGTTAAACGAAT
GAATCATCTGTTCATGCATGCTCTACTTTGATATTATAACCTATGTCACATGTGTTTAATAAATACCATATATTTTGTTCTACTAA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K
RYLS*KER
IMNYKAGWTI*QKPRPRLKWKPER*E
WAVIFYPAKQAGLVKL*CLLGTTPHTQESWS*P
*RKL*LRHSEAYTFYRWTKALEPCGFKSFGKGD
CCFPYTQCKPEWESLRL*STSKTGRKVKAE*AGK
RGIYKVTFQTPTRHTLPYCIIICFLCRH*NPIR
RIPSQCILFARLWLGGKRTLI*SFMLFMPGLFD
RA*RFCLPIKVLEAAFL*YREACPQEAWAPSQT*
TWKGGFPACRCSFLLVPSICAGSWEPH*ETCVG
QTSFILMR*SLGLICALCFHWTS*NHCIYSTSD
FFFFLTLT*REFYMSWKFNNLVFSVSIGVFVKRM
NHLFMHALL*YYNLCHMCLINTIYFVLL

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGD*IIKEKVATETALNTAAFRK
DIFLRRR
E**TTKQVGLFNRNPGQD*SGNQRDR
SGL*SSTQPNRQDS*NCDAF*ELHPIHKSPGVNH
EENSDLGTQRHTLFTDGQKLWNPVASNPLGRVT
VVSPTHSVSRNGNR*GSDPLLRQEGK*RQSEQV
REGYTRSHFRHPLGIPCRTASSFVFFVDTEILSG
GFLHNVFYLLDFGWEGKGH*FEVSCYSCQDCLI
EHEGFVYP*KY*RQRFSDTERPVHKKHGHPAKL
EPGREGSRPAGALSSWSQASVQGRGSHTERLVWA
RQASF*CASPLV*FVPYAFIGPAEITVFIQLVI
FFSFSL*LKENFICLGNLII*CSQYQLVFLLNE
*IICSCMLYFDIITYVTCV**IPYILFY*

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK
ISFLEGE
NNELQSRLDYLTETQAKTEVETREIG
VGCDLLPSQTGRTREIVMPSRNYTPYTRVLELT
MKKTLT*ALRGIHFLQMDKSSGTLWLQILWEG*L
LFPLHTV*AGMGIAEALIHF*DRKESEGRVSR*
ERDIQGHISDTHSAYPAVLHHHLFSL*TLKSYQ
EDSFTMYFIC*TLVGREKDINLKFHVIHARIV**
SMKVLFTHKSIRGSVSLIQRGLSTRSMGTQPNL
NLEGRVPGLQVLFPLGPKHLCRVVGATLRDLCG
PDKLHSDALVPWFNLCLMLSLDQLKSLYLFN**F
FFLSHFNLKRILYVLEI**FSVLSINWCFC*TN
ESSVHACSTLIL*PMSHVFNKYHIFCST

Sequence NM_001018090

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA
GATATCTTTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA
GTGGGCTGTGATCTTCTACCCAGGTTACCTTTTCGACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGC
GCAGGGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTGCTCTCCATAGAAGAATCCTTGGCACT
TCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGCTCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCA
GAAGGGGAGTCTTCAGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGAAGATAATCTCCTAAATCACTGACGTTGA
GATGTCATCATCTTACATCAGACTTTCTAACTAGTATCAAGATCAGTGTCAGATATTGTTGAGGGAAGTAATTTTATAAAGTTACACAAAGGTAGTTATA
AAAAAAGCCCAGTTTGTCTTTCAGAAGGTGACTTTCATGTGCTTGAAAAGTTTAATATTTGAATATTGTGTTTAACCACATGGTATTAAAATTTTGCAAT
ATATTGTGTATTGGTCTGATATTTTAGTATATAGTAGAACATACTTTTTTTTTCTTTAAGCCAAATGAAAAGAGGTAACTTTGCTTTTTTCCTTTTTCTT
ACCTATCAAATAGCATTTATTACATGTCTTTCAGTGAAATACTTAGTTGTTCCAGGCACCTAAAATCAATTAGGAAGACATAGTTCCCTTCTTTTTTGGG
TAATGAAGGGAGCAGTCTAAAGAATTGTATGCATGTTTGCATGGGGTTATTGAGACATTGGATGTGAAATACCTAGGAAGACATGTATGGAGAATGCTTA
ACAAATGCTCTTCTCCTTTTTCTGTCTTCCCTTAGGAAGAACGTTATCTCTCTTCTGATTAGGAAGGACTTCCCTTTTGAGTAGATGCTGGAAAGTGGAG
GTTTTTTTTGTTGGTTTGTTTGTTTGGTTTTGTGTTTTTTTTTGAGATGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCAGGATCTCGGCTCA
CTGCAATCTAGGTGGAGGTCTTTTTAAATAACCTCATGTTACTTCAGGCAAATCTGGTACAGGAGAAGCCCAAGCTAACTGAGCTGGGTGAAGGCTTGTG
CATCACCTGGCTTGAGTTCCTTGCTGTCACAGATGATAGGTTCATGTACACAACATTAATGTTTGATAAGGAAAGCATTTTTTTTTTGTTAAAATTGAAT
TGTCAGTACTTTACTTTTTTCCCCCCAAATAGGTATATATAGACTGCAGAAGCTTCAGTCCCATATATAAACGTTCATGTCATTTTAGAGGATTATATGG
TTGTCTCCAGAGAAATTAACTTACATTATCAAAAAATTCTTTGTCTAATATATTGGAGTCTTTAAAAGTGTTGAAGAATAAAATATTAATTAGAACAACT
TCAAATGCTAAGGTGATGTTTTACCGGGACTTAATTAAATACAACTTTTTCCTTTCGAAAAAGACTGTTCACATTTGCTTCCAGGTGTAATTATTGCTTT
CATTCCTTACCCCCCTCAAGCAAATGTGAAAAGTATACTGACCTAAGATTCTCATTAGTTTTAGTTCTTAAAACGAATAGTAAATGATTTCACAGCGTAA
AATTTGATGTTATTTCTGGAAGCTGGATGATATGGAAGAGTTCATGTGCCTGGACACACAGTTCCCTGTAATCCTGCCATCTAGGGAGATAAACTCTGTT
AACATTTTGATGTTGTGTTTTTGCTTATTTTTCTATGCATAGTTATGCATTTATATTCTCAAAAATGAAATCATAATGTACATTGCTTTGTAGTCTGCTC
TATATATTGTCAATATGTTCCCATATTAAATATTTTGCTTTTTAGACAGCTGTAGGATATTTTATTTCATGGGTATACCATAACTTTAAGATTTATTTCC
TATTCAGCAATTTGATTATGTATTATAATGTAATCTGTAAATAGTGATATGATTAACATCTTAATCACATCCATAGAAAATGACTTATTTCCAGAAGATA
CCGCTCTAAAAATGAAATTGTTAGGTGAAAGTGTTTTTGAGAATTTTTATATATATTACTCACTGCAGAGATCATGTTCATTTACATTCCCATTAGCAAT
ATATCCAATGCTCAGAATTGAAAATTACTACTTAAAATCTTTGCAAATTGAATAATTACAGAAAAGGGCTACATTTAAAATTTGTATTTGTTTTTCTTAC
TTAGTGAAGGTTGTGTTTTTTTTCATATTCATTGGCCATGCTATATTCATTCTTTTGAGAATTGCTCATTCATATTTTTTCTGTTGCTTGCTTGGAGAAT
TTGCTCTTTTTTTGGCATATAAGTTCTTAAGAATGTCCGTCCTTTGTCATATATTGTAACTTTTTTTAGCCTTATTTGTCATTTAACATAGTTTGTGGTA
TTTCAGTATAGAAGTTGTTTTCTTTAGGAGTCTACCTTTGTGTATGTGCTTGAAAGTATTTTTGTAATCCAAGAATGGATAAATGTCCTTATTTTCTTCT
AGTATTTTCTTATTTCACTTTTACACCTATCTTTAAAAGGAATTTAAATTTTGTTTATATACATTAACTAAATCAGTACAAGTTTGGGGTGAAAAGGAAA
TTTTTAATCCATCTCAAATGTATTTGGTTATATTATATAAGGTAGGGATTTGATTTTATTTCGAAGAATTTACCAATTGGTTCAGTATGTGAAACCACCA
TTTCCCCTCTGATTAGTAATGCTATTTTTATAGAATATTAAATTCTTATGTGTCTTTTGTGTCTTAGGCCAATTTCTGGGCCTTCTGGGTTTTTTCTACA
CTGTCAATAATTATCGCTTTAATTGGTGTTAATATTTGGTAGTACACAGGTTCTCCCTTGTTCTTTTAAATTTTGTGAAATATAACTTACAAATAAGTAC
TTAAAACAGAGCTTAATGGGTAAAGCAACAGCTGTGTAATCACAAACTAGATGCAGAGGAGGTCTTGCCAGCACTCCAAAATCTCTGTGTGTCCCCTCTT
GACACTCACTGTTTCCCCCAGAGCTAACTATCCTGACGTATGGTAATTCTCCCTCTGTCCTTTTAAGAATTATTTTACCAGTTATGTGTATAACAATATG
GTTTACATTTTGCTCACTTTTGACTTATATAAAAATAGAATAATACTA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K
RYLS*KER
IMNYKAGWTI*QKPRPRLKWKPER*E
WAVIFYPGYLFDKMIHLVIARRVGLLFPQKSGG
AGISSILMTSQQLGFYHFTICPRSCSP*KNPWH
FRNSRNRIMRRCKQSSQRKN*LKD*ILKCGVIIQ
KGSLQGDTEK*GMKMTIGPLMNSEDNLLNH*R*
DVIILHQTF*LVSRSVSDIVEGSNFIKLHKGSY
KKSPVCLSEGDFHVLEKFNI*ILCLTTWY*NFAI
YCVLV*YFSI**NILFFSLSQMKRGNFAFFLFL
TYQIAFITCLSVKYLVVPGT*NQLGRHSSLLFW
VMKGAV*RIVCMFAWGY*DIGCEIPRKTCMENA*
QMLFSFFCLPLGRTLSLF*LGRTSLLSRCWKVE
VFFVGLFVWFCVFF*DGVLLCRPGWSAVAGSRL
TAI*VEVFLNNLMLLQANLVQEKPKLTELGEGLC
ITWLEFLAVTDDRFMYTTLMFDKESIFFLLKLN
CQYFTFFPPNRYI*TAEASVPYINVHVILEDYM
VVSREINLHYQKILCLIYWSL*KC*RIKY*LEQL
QMLR*CFTGT*LNTTFSFRKRLFTFASRCNYCF
HSLPPSSKCEKYTDLRFSLVLVLKTNSK*FHSV
KFDVISGSWMIWKSSCAWTHSSL*SCHLGR*TLL
TF*CCVFAYFSMHSYAFIFSKMKS*CTLLCSLL
YILSICSHIKYFAF*TAVGYFISWVYHNFKIYF
LFSNLIMYYNVICK**YD*HLNHIHRK*LISRRY
RSKNEIVR*KCF*EFLYILLTAEIMFIYIPISN
ISNAQN*KLLLKIFAN*IITEKGYI*NLYLFFL
LSEGCVFFHIHWPCYIHSFENCSFIFFLLLAWRI
CSFFGI*VLKNVRPLSYIVTFFSLICHLT*FVV
FQYRSCFL*ESTFVYVLESIFVIQEWINVLIFF
*YFLISLLHLSLKGI*ILFIYIN*ISTSLG*KGN
F*SISNVFGYII*GRDLILFRRIYQLVQYVKPP
FPL*LVMLFL*NIKFLCVFCVLGQFLGLLGFFY
TVNNYRFNWC*YLVVHRFSLVLLNFVKYNLQIST
*NRA*WVKQQLCNHKLDAEEVLPALQNLCVSPL
DTHCFPQS*LS*RMVILPLSF*ELFYQLCV*QY
GLHFAHF*LI*K*NNT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGD*IIKEKVATETALNTAAFRK
DIFLRRR
E**TTKQVGLFNRNPGQD*SGNQRDR
SGL*SSTQVTFSTK*FI*SLPEEWVSYFLRRAAA
QG*AAS**HHSSSASTTSPYAHAAALHRRILGT
SETAETEL*GDASKARSAKIS*KTEY*NAEL*S
RRGVFREIPRSKG*R*RLVL**ILKIIS*ITDVE
MSSSYIRLSN*YQDQCQILLREVIL*SYTKVVI
KKAQFVFQKVTFMCLKSLIFEYCV*PHGIKILQ
YIVYWSDILVYSRTYFFFL*AK*KEVTLLFSFFL
PIK*HLLHVFQ*NT*LFQAPKIN*EDIVPFFFG
**REQSKELYACLHGVIETLDVKYLGRHVWRML
NKCSSPFSVFP*EERYLSSD*EGLPF*VDAGKWR
FFLLVCLFGFVFFFEMGSCSVAQAGVQWQDLGS
LQSRWRSF*ITSCYFRQIWYRRSPS*LSWVKAC
ASPGLSSLLSQMIGSCTQH*CLIRKAFFFC*N*I
VSTLLFSPQIGIYRLQKLQSHI*TFMSF*RIIW
LSPEKLTYIIKKFFV*YIGVFKSVEE*NIN*NN
FKC*GDVLPGLN*IQLFPFEKDCSHLLPGVIIAF
IPYPPQANVKSILT*DSH*F*FLKRIVNDFTA*
NLMLFLEAG*YGRVHVPGHTVPCNPAI*GDKLC
*HFDVVFLLIFLCIVMHLYSQK*NHNVHCFVVCS
IYCQYVPILNILLFRQL*DILFHGYTITLRFIS
YSAI*LCIIM*SVNSDMINILITSIENDLFPED
TALKMKLLGESVFENFYIYYSLQRSCSFTFPLAI
YPMLRIENYYLKSLQIE*LQKRATFKICICFSY
LVKVVFFFIFIGHAIFILLRIAHSYFFCCLLGE
FALFLAYKFLRMSVLCHIL*LFLALFVI*HSLWY
FSIEVVFFRSLPLCMCLKVFL*SKNG*MSLFSS
SIFLFHFYTYL*KEFKFCLYTLTKSVQVWGEKE
IFNPSQMYLVILYKVGI*FYFEEFTNWFSM*NHH
FPSD**CYFYRILNSYVSFVS*ANFWAFWVFST
LSIIIALIGVNIW*YTGSPLFF*IL*NITYK*V
LKTELNG*SNSCVITN*MQRRSCQHSKISVCPLL
TLTVSPRANYPDVW*FSLCPFKNYFTSYVYNNM
VYILLTFDLYKNRIIL

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK
ISFLEGE
NNELQSRLDYLTETQAKTEVETREIG
VGCDLLPRLPFRQNDSSSHCQKSGSPISSEERR
RRDKQHLDDITAARLLPLHHMPTQLLSIEESLAL
QKQQKQNYEEMQAKLAAQKLAERLNIKMRSYNP
EGESSGRYREVRDEDDDWSSDEF*R*SPKSLTL
RCHHLTSDFLTSIKISVRYC*GK*FYKVTQR*L*
KKPSLSFRR*LSCA*KV*YLNIVFNHMVLKFCN
ILCIGLIF*YIVEHTFFFFKPNEKR*LCFFPFS
YLSNSIYYMSFSEILSCSRHLKSIRKT*FPSFLG
NEGSSLKNCMHVCMGLLRHWM*NT*EDMYGECL
TNALLLFLSSLRKNVISLLIRKDFPFE*MLESG
GFFCWFVCLVLCFFLRWGLALSPRLECSGRISAH
CNLGGGLFK*PHVTSGKSGTGEAQAN*AG*RLV
HHLA*VPCCHR**VHVHNINV**GKHFFFVKIE
LSVLYFFPPK*VYIDCRSFSPIYKRSCHFRGLYG
CLQRN*LTLSKNSLSNILESLKVLKNKILIRTT
SNAKVMFYRDLIKYNFFLSKKTVHICFQV*LLL
SFLTPLKQM*KVY*PKILISFSS*NE**MISQRK
I*CYFWKLDDMEEFMCLDTQFPVILPSREINSV
NILMLCFCLFFYA*LCIYILKNEIIMYIAL*SA
LYIVNMFPY*IFCFLDSCRIFYFMGIP*L*DLFP
IQQFDYVL*CNL*IVI*LTS*SHP*KMTYFQKI
PL*K*NC*VKVFLRIFIYITHCRDHVHLHSH*Q
YIQCSELKITT*NLCKLNNYRKGLHLKFVFVFLT
**RLCFFSYSLAMLYSFF*ELLIHIFSVACLEN
LLFFWHISS*ECPSFVIYCNFF*PYLSFNIVCG
ISV*KLFSLGVYLCVCA*KYFCNPRMDKCPYFLL
VFSYFTFTPIFKRNLNFVYIH*LNQYKFGVKRK
FLIHLKCIWLYYIR*GFDFISKNLPIGSVCETT
ISPLISNAIFIEY*ILMCLLCLRPISGPSGFFLH
CQ*LSL*LVLIFGSTQVLPCSFKFCEI*LTNKY
LKQSLMGKATAV*SQTRCRGGLASTPKSLCVPS
*HSLFPPELTILTYGNSPSVLLRIILPVMCITIW
FTFCSLLTYIKIE*Y

Sequence NM_152451

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA
GATATCTTTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA
GTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGAGTCCTGGAGTTAACCA
TGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCCTTTGGGAAGGGTGACT
GTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGGCAGAGTGAGCAGGCAA
AGTAGAAAATTCGAAAAGGTCCTCAACGAGTTTGTACAGCTGCTCCCACTCCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATT
TTGGATTGTTGCAATGACTTGGGAGTGCCATAGCACCTAGCAGGCAGAAGCCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAA
GTCCTTCTCCAAATGCATTGAGCCCCAAGAAAAATCGCTGATTCTGAGGAACCTTCCAACTTTAACAATTTCAGGACGTCCCTAAGCTGAGCTAGGAGGC
ACCACGCACAGGCCGGAGGCACCACGCACAGGCCAGAGTCTCCCAAATCCTGTCCCGCACTCTCTCCCACCTTATTAATGGCCAGCTCATCTTTCAAGTT
TCGACTTGAACCACTTCTCAGAGATATCTTCCCAGGTGCCCATCCCAACGTTACTATTATATCATAATTTGATTCTCCCTCCCCCCATCCCCCCGCCAAC
TCTGTAAACCCCAAGTATAACACAGGACGTTGCTATTTGTTGACTGACACATAGTGCTTGGTGAGGTCACGCTGGCGCTGAATACTTTTTGTATAAACCG
ACGAACAGCTGATGACCGCATGAATGCTCACACAAGGGGAAAGGGCGCCAAGCCAAGGAGCCGGCGTCATCCGACGTAACTTCCGCCCTTTAACGCTCCT
GCGGACGCCCCCTTCTCCCAGTCCGTGTTTGTTTCCGGAATTTCAATAAAGCTCGATTCGGCTCGAAGAAGACCCCGTTCTTCCGGGAAAATGGCGACTC
CCGCTCGTGCCCCGGAGTCACCGCCGTCCGCGGATCCGGCGCTAGTAGCGGGGCCTGCCGAGGAAGCCGAGTGCCCGCCGCCGCGCCAGCCTCAGCCCGC
GCAGAATGTGCTCGCTGCCCCGCGGCTTCGAGCCCCAAGCTCCCGAGGACTTGGCGCAGCGGAGTTTGGTGGAGCTGCGGGAAATGTTGAAGCGCCAGGA
GAGACTTTTGCGCAACGA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K
RYLS*KER
IMNYKAGWTI*QKPRPRLKWKPER*E
WAVIFYPAKQAGLVKL*CLLGTTPHTQESWS*P
*RKL*LRHSEAYTFYRWTKALEPCGFKSFGKGD
CCFPYTQCKPEWESLRL*STSKTGRKVKAE*AGK
VENSKRSSTSLYSCSHSLITSCGPLEMYVGGDI
LDCCNDLGVP*HLAGRSQKHQMS*QCLRRCYK*
SPSPNALSPKKNR*F*GTFQL*QFQDVPKLS*EA
PRTGRRHHAQARVSQILSRTLSHLINGQLIFQV
ST*TTSQRYLPRCPSQRYYYIII*FSLPPSPRQ
LCKPQV*HRTLLFVD*HIVLGEVTLALNTFCINR
RTADDRMNAHTRGKGAKPRSRRHPT*LPPFNAP
ADAPFSQSVFVSGISIKLDSARRRPRSSGKMAT
PARAPESPPSADPALVAGPAEEAECPPPRQPQPA
QNVLAAPRLRAPSSRGLGAAEFGGAAGNVEAPG
ETFAQR

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGD*IIKEKVATETALNTAAFRK
DIFLRRR
E**TTKQVGLFNRNPGQD*SGNQRDR
SGL*SSTQPNRQDS*NCDAF*ELHPIHKSPGVNH
EENSDLGTQRHTLFTDGQKLWNPVASNPLGRVT
VVSPTHSVSRNGNR*GSDPLLRQEGK*RQSEQA
K*KIRKGPQRVCTAAPTPSSPLVGLWKCMLEETF
WIVAMTWECHST*QAEARNTKCLNNA*GGATNE
VLLQMH*APRKIADSEEPSNFNNFRTSLS*ARR
HHAQAGGTTHRPESPKSCPALSPTLLMASSSFKF
RLEPLLRDIFPGAHPNVTIIS*FDSPSPHPPAN
SVNPKYNTGRCYLLTDT*CLVRSRWR*ILFV*T
DEQLMTA*MLTQGERAPSQGAGVIRRNFRPLTLL
RTPPSPSPCLFPEFQ*SSIRLEEDPVLPGKWRL
PLVPRSHRRPRIRR**RGLPRKPSARRRASLSP
RRMCSLPRGFEPQAPEDLAQRSLVELREMLKRQE
RLLRN

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK
ISFLEGE
NNELQSRLDYLTETQAKTEVETREIG
VGCDLLPSQTGRTREIVMPSRNYTPYTRVLELT
MKKTLT*ALRGIHFLQMDKSSGTLWLQILWEG*L
LFPLHTV*AGMGIAEALIHF*DRKESEGRVSRQ
SRKFEKVLNEFVQLLPLPHHLLWAFGNVCWRRH
FGLLQ*LGSAIAPSRQKPETPNVLTMLEAVLQMK
SFSKCIEPQEKSLILRNLPTLTISGRP*AELGG
TTHRPEAPRTGQSLPNPVPHSLPPY*WPAHLSS
FDLNHFSEISSQVPIPTLLLYHNLILPPPIPPPT
L*TPSITQDVAIC*LTHSAW*GHAGAEYFLYKP
TNS**PHECSHKGKGRQAKEPASSDVTSAL*RS
CGRPLLPVRVCFRNFNKARFGSKKTPFFRENGDS
RSCPGVTAVRGSGASSGACRGSRVPAAAPASAR
AECARCPAASSPKLPRTWRSGVWWSCGKC*SAR
RDFCAT

Sequence NM_001018096

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCGAATGTATGGAGACTATGATGAGATGAGACAG
AAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCATGCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTA
ATGCCATGAACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAAAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAA
TCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTG
GAATCGTCCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACA
GTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGA
GAAAGACCAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATG
TCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGC
ATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAA
GATATCTTTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGA
GTGGGCTGTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGAGTCCTGGAGTTAACCA
TGAAGAAAACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCCTTTGGGAAGGGTGACT
GTTGTTTCCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGGCAGAGTGAGCAGGGAA
GGAAAGGCCTAAAAGGGAGACTAAAGATGAGTTGCTAAAAAAGAGAGAACAGAAGCAAAGAGACCAGAAGCCATGAAGGGATCCATGGTGCTTGCAAGAT
GGTGAAGAAATGGAGTCAGAGAATGTGGACAATTCTTTTAGGACGCGTATCTCTGAAAAAAGAAGGCAGGCAAAGTAGAAAATTCGAAAAGGTCCTCAAC
GAGTTTGTACAGCTGCTCCCACTCCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATTTTGGATTGTTGCAATGACTTGGGAGTG
CCATAGCACCTAGCAGGCAGAAGCCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAAGTCCTTCTCCAAATGCATTGACCCCAA
GCTCCCGAGGACTTGGCGCAGCGGAGTTTGGTGGAGCTGCGGGAAATGTTGAAGCGCCAGGAGAGACTTTTGCGCAACGA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKM*EPLWKR*ESECMETMMR*DR
RFDSSPRNYQFPMLSRSIWRITSKPSRLPWIVL
MP*TQPWHQIPLACKKPSWM*LWKTATLRIKSE
ICSRRMKHPWTS*GKSRGSWR*RKLKTSC*K*RW
NRPKKPMLR*CER*PRSCTASMRRSCRKNRGST
VLRRRLFWKKPIVF*KRLKKPIKRCKQQRSA*R
RKTRGSGSWTG*LSAWKRNVINCNFNS*NMKQKC
LGS*LILTRKGISSWRRHQPASVSGSDT*MTWC
IASRRKSSRWSRRLNH*RKSCNRNSS*YCSF*K
RYLS*KER
IMNYKAGWTI*QKPRPRLKWKPER*E
WAVIFYPAKQAGLVKL*CLLGTTPHTQESWS*P
*RKL*LRHSEAYTFYRWTKALEPCGFKSFGKGD
CCFPYTQCKPEWESLRL*STSKTGRKVKAE*AGK
ERPKRETKDELLKKREQKQRDQKP*RDPWCLQD
GEEMESENVDNSFRTRISEKRRQAK*KIRKGPQ
RVCTAAPTPSSPLVGLWKCMLEETFWIVAMTWEC
HST*QAEARNTKCLNNA*GGATNEVLLQMH*PQ
APEDLAQRSLVELREMLKRQERLLRN

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQRCESHFGKGEKANVWRL**DET
EDSTAHPGTISFPCSAGVSGESHPNPVVCPGSF*
CHELSLGIRFHWPAKNPRGCDFGKQQH*GSNQK
SAADV*SIHGQAEGKAEAVGGSAS*KPAAKNEG
GIVPRSQC*GDARDDQEAVQPV*GEAAGRTEEAQ
C*EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRG
ERPEDRGAGQAD*AHGKGTSSTATSTPRT*NRN
VWGVN*F*QGKVSAVGGGISQPP*ADQTPR*HGA
LPAEESQADGRGD*IIKEKVATETALNTAAFRK
DIFLRRR
E**TTKQVGLFNRNPGQD*SGNQRDR
SGL*SSTQPNRQDS*NCDAF*ELHPIHKSPGVNH
EENSDLGTQRHTLFTDGQKLWNPVASNPLGRVT
VVSPTHSVSRNGNR*GSDPLLRQEGK*RQSEQG
RKGLKGRLKMSC*KRENRSKETRSHEGIHGACKM
VKKWSQRMWTILLGRVSLKKEGRQSRKFEKVLN
EFVQLLPLPHHLLWAFGNVCWRRHFGLLQ*LGS
AIAPSRQKPETPNVLTMLEAVLQMKSFSKCIDPK
LPRTWRSGVWWSCGKC*SARRDFCAT

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDVRATLEKVRKRMYGDYDEMRQ
KIRQLTQELSVSHAQQEYLENHIQTQSSALDRF
NAMNSALASDSIGLQKTLVDVTLENSNIKDQIRN
LQQTYEASMDKLREKQRQLEVAQVENQLLKMKV
ESSQEANAEVMREMTKKLYSQYEEKLQEEQRKH
SAEKEALLEETNSFLKAIEEANKKMQAAEISLEE
KDQRIGELDRLIERMEKERHQLQLQLLEHETEM
SGELTDSDKERYQQLEEASASLRERIRHLDDMV
HCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEK
ISFLEGE
NNELQSRLDYLTETQAKTEVETREIG
VGCDLLPSQTGRTREIVMPSRNYTPYTRVLELT
MKKTLT*ALRGIHFLQMDKSSGTLWLQILWEG*L
LFPLHTV*AGMGIAEALIHF*DRKESEGRVSRE
GKA*KGD*R*VAKKERTEAKRPEAMKGSMVLAR
W*RNGVRECGQFF*DAYL*KKKAGKVENSKRSST
SLYSCSHSLITSCGPLEMYVGGDILDCCNDLGV
P*HLAGRSQKHQMS*QCLRRCYK*SPSPNALTP
SSRGLGAAEFGGAAGNVEAPGETFAQR

Sequence NM_001018099

GCTGAGGCTGCAAGTAGCCGGCGCCGTCCCGCGTCGCCCCCGCGCAGGGCGGGCCCCGCACGCTTATTCTGCCCGGGAGGAACGCCGGCGTCCAGCCCGC
TACCGACCGCCGCTGCGGGATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCAGGAGGGCAAAT
GTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAAGAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATG
GAGAACCTACCAGGAAACTTCCTCAGGGTGTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCAC
CAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAACATTAAGGATCAAATCAGAAATCTGCAG
CAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAGAGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTGGAATCGT
CCCAAGAAGCCAATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACAGAGGAAGCACAGTGCTGA
GAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAAGCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGAGAAAGAC
CAGAGGATCGGGGAGCTGGACAGGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAATGTCTGGGG
AGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGTGAGCGGATCAGACACCTAGATGACATGGTGCATTGCCA
GCAGAAGAAAGTCAAGCAGATGGTCGAGGAGATTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAAGATATCT
TTCTTAGAAGGAGAG
AATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACTGAAGTGGAAACCAGAGAGATAGGAGTGGGCT
GTGATCTTCTACCCAGCCAAACAGGCAGGACTCGTGAAATTGTGATGCCTTCTAGGAACTACACCCCATACACAAGAGTCCTGGAGTTAACCATGAAGAA
AACTCTGACTTAGGCACTCAGAGGCATACACTTTTTACAGATGGACAAAAGCTCTGGAACCCTGTGGCTTCAAATCCTTTGGGAAGGGTGACTGTTGTTT
CCCCTACACACAGTGTAAGCCGGAATGGGAATCGCTGAGGCTCTGATCCACTTCTAAGACAGGAAGGAAAGTGAAGGCAGAGTGAGCAGGCAAAGTAGAA
AATTCGAAAAGGTCCTCAACGAGTTTGTACAGCTGCTCCCACTCCCTCATCACCTCTTGTGGGCCTTTGGAAATGTATGTTGGAGGAGACATTTTGGATT
GTTGCAATGACTTGGGAGTGCCATAGCACCTAGCAGGCAGAAGCCAGAAACACCAAATGTCTTAACAATGCTTGAGGCGGTGCTACAAATGAAGTCCTTC
TCCAAATGCATTGAGCCCCAAGAAAAATCGCTGATTCTGAGGAACCTTCCAACTTTAACAATTTCAGGACGTCCCTAAGCTGAGCTAGGAGGCACCACGC
ACAGGCCGGAGGCACCACGCACAGGCCAGAGTCTCCCAAATCCTGTCCCGCACTCTCTCCCACCTTATTAATGGCCAGCTCATCTTTCAAGTTTCGACTT
GAACCACTTCTCAGAGATATCTTCCCAGTGCTTGGTGAGGTCACGCTGGCGCTGAATACTTTTTGTATAAACCGACGAACAGCTGATGACCGCATGAATG
CTCACACAAGGGGAAAGGGCGCCAAGCCAAGGAGCCGGCGTCATCCGACGTAACTTCCGCCCTTTAACGCTCCTGCGGACGCCCCCTTCTCCCAGTCCGT
GTTTGTTTCCGGAATTTCAATAAAGCTCGATTCGGCTCGAAGAAGACCCCGTTCTTCCGGGAAAATGGCGACTCCCGCTCGTGCCCCGGAGTCACCGCCG
TCCGCGGATCCGGCGCTAGTAGCGGGGCCTGCCGAGGAAGCCGAGTGCCCGCCGCCGCGCCAGCCTCAGCCCGCGCAGAATGTGCTCGCTGCCCCGCGGC
TTCGAGCCCCAAGCTCCCGAGGACTTGGCGCAGCGGAGTTTGGTGGAGCTGCGGGAAATGTTGAAGCGCCAGGAGAGACTTTTGCGCAACGA
  • refseq_Gcom1.F3 (forward primer)
  • refseq_Gcom1.R3 (reverse primer)
  • Single exon skipping

Frame 0

AEAASSRRRPASPPRRAGPARLFCPGGTPASSP
LPTAAAGCCAPRPRSPCSRAAPPGRPGRPAGGQM
FADYG*PYLLRVQFLSNVKRRLREKSSFLT*AM
ENLPGNFLRVLFMVWCEDQIKISRKKWWCMGGP
PVS*KKR*TTSKIKPSWM*LWKTATLRIKSEICS
RRMKHPWTS*GKSRGSWR*RKLKTSC*K*RWNR
PKKPMLR*CER*PRSCTASMRRSCRKNRGSTVL
RRRLFWKKPIVF*KRLKKPIKRCKQQRSA*RRKT
RGSGSWTG*LSAWKRNVINCNFNS*NMKQKCLG
S*LILTRKGISSWRRHQPASVSGSDT*MTWCIA
SRRKSSRWSRRLNH*RKSCNRNSS*YCSF*KRYL
S*KER
IMNYKAGWTI*QKPRPRLKWKPER*EWA
VIFYPAKQAGLVKL*CLLGTTPHTQESWS*P*R
KL*LRHSEAYTFYRWTKALEPCGFKSFGKGDCCF
PYTQCKPEWESLRL*STSKTGRKVKAE*AGKVE
NSKRSSTSLYSCSHSLITSCGPLEMYVGGDILD
CCNDLGVP*HLAGRSQKHQMS*QCLRRCYK*SPS
PNALSPKKNR*F*GTFQL*QFQDVPKLS*EAPR
TGRRHHAQARVSQILSRTLSHLINGQLIFQVST
*TTSQRYLPSAW*GHAGAEYFLYKPTNS**PHEC
SHKGKGRQAKEPASSDVTSAL*RSCGRPLLPVR
VCFRNFNKARFGSKKTPFFRENGDSRSCPGVTA
VRGSGASSGACRGSRVPAAAPASARAECARCPAA
SSPKLPRTWRSGVWWSCGKC*SARRDFCAT

Frame 1

LRLQVAGAVPRRPRAGRAPHAYSAREERRRPAR
YRPPLRDAALHVHGHPALGRRRQDARGAQQEGK
CLQTTADRTS*ESSS*AM*KED*EKRAAS*PEQW
RTYQETSSGCCLWCGAKIRSKSAERNGGVWVVH
QSAERRDELHQR*NPRGCDFGKQQH*GSNQKSA
ADV*SIHGQAEGKAEAVGGSAS*KPAAKNEGGIV
PRSQC*GDARDDQEAVQPV*GEAAGRTEEAQC*
EGGSFGRNQ*FSESD*RSQ*KDASSRDQPRGER
PEDRGAGQAD*AHGKGTSSTATSTPRT*NRNVWG
VN*F*QGKVSAVGGGISQPP*ADQTPR*HGALP
AEESQADGRGD*IIKEKVATETALNTAAFRKDI
FLRRR
E**TTKQVGLFNRNPGQD*SGNQRDRSGL
*SSTQPNRQDS*NCDAF*ELHPIHKSPGVNHEE
NSDLGTQRHTLFTDGQKLWNPVASNPLGRVTVV
SPTHSVSRNGNR*GSDPLLRQEGK*RQSEQAK*K
IRKGPQRVCTAAPTPSSPLVGLWKCMLEETFWI
VAMTWECHST*QAEARNTKCLNNA*GGATNEVL
LQMH*APRKIADSEEPSNFNNFRTSLS*ARRHHA
QAGGTTHRPESPKSCPALSPTLLMASSSFKFRL
EPLLRDIFPVLGEVTLALNTFCINRRTADDRMN
AHTRGKGAKPRSRRHPT*LPPFNAPADAPFSQSV
FVSGISIKLDSARRRPRSSGKMATPARAPESPP
SADPALVAGPAEEAECPPPRQPQPAQNVLAAPR
LRAPSSRGLGAAEFGGAAGNVEAPGETFAQR

Frame 2

*GCK*PAPSRVAPAQGGPRTLILPGRNAGVQPA
TDRRCGMLRSTSTVTLLSGGAARTPGAPSRRAN
VCRLRLTVPPESPVPEQCEKKIERKEQLLDLSN
GEPTRKLPQGVVYGVVRRSDQNQQKEMVVYGWST
SQLKEEMNYIKDKTLVDVTLENSNIKDQIRNLQ
QTYEASMDKLREKQRQLEVAQVENQLLKMKVES
SQEANAEVMREMTKKLYSQYEEKLQEEQRKHSAE
KEALLEETNSFLKAIEEANKKMQAAEISLEEKD
QRIGELDRLIERMEKERHQLQLQLLEHETEMSG
ELTDSDKERYQQLEEASASLRERIRHLDDMVHCQ
QKKVKQMVEEIESLKKKLQQKQLLILQLLEKIS
FLEGE
NNELQSRLDYLTETQAKTEVETREIGVG
CDLLPSQTGRTREIVMPSRNYTPYTRVLELTMKK
TLT*ALRGIHFLQMDKSSGTLWLQILWEG*LLF
PLHTV*AGMGIAEALIHF*DRKESEGRVSRQSR
KFEKVLNEFVQLLPLPHHLLWAFGNVCWRRHFGL
LQ*LGSAIAPSRQKPETPNVLTMLEAVLQMKSF
SKCIEPQEKSLILRNLPTLTISGRP*AELGGTT
HRPEAPRTGQSLPNPVPHSLPPY*WPAHLSSFDL
NHFSEISSQCLVRSRWR*ILFV*TDEQLMTA*M
LTQGERAPSQGAGVIRRNFRPLTLLRTPPSPSP
CLFPEFQ*SSIRLEEDPVLPGKWRLPLVPRSHRR
PRIRR**RGLPRKPSARRRASLSPRRMCSLPRG
FEPQAPEDLAQRSLVELREMLKRQERLLRN

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