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Sequences

Sequence NM_152998

CAAATAAAAGCGATGGCGATTGGGCTGCCGCGTTTGGCGCTCGGTCCGGTCGCGTCCGACACCCGGTGGGACTCAGAAGGCAGTGGAGCCCCGGCGGCGG
CGGCGGCGGCGCGCGGGGGCGACGCGCGGGAACAACGCGAGTCGGCGCGCGGGACGAAGAATAATCATGGGCCAGACTGGGAAGAAATCTGAGAAGGGAC
CAGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACATGCGACTGAGACAGCTCAAGAGGTTCAGACGAGCTGATGAAGTAAAGAGTATGTTTAGTTCCAA
TCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAATGGAAACAGCGAAGGATACAGCCTGTGCACATCCTGACTTCTGTGAGCTCATTGCGC
GGGACTAGGGAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGAGATGAAGTTTTAGATCAGGATGGTACTTTCATTGAAGAACTAATAA
AAAATTATGATGGGAAAGTACACGGGGATAGAGAATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGCCCTTGGTCAATATAATGATGA
TGACGATGATGATGATGGAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCACCGAGATGATAAAGAAAGCCGCCCACCTCGGAAA
TTTCCTTCTGATAAAATTTTTGAAGCCATTTCCTCAATGTTTCCAGATAAGGGCACAGCAGAAGAACTAAAGGAAAAATATAAAGAACTCACCGAACAGC
AGCTCCCAGGCGCACTTCCTCCTGAATGTACCCCCAACATAGATGGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTACACTCCTTTCATACGCT
TTTCTGTAGGCGATGTTTTAAATATGACTGCTTCCTACATCCTTTTCATGCAACACCCAACACTTATAAGCGGAAGAACACAGAAACAGCTCTAGACAAC
AAACCTTGTGGACCACAGTGTTACCAGCATTTGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGCTGAGCGGATAAAGACCCCACCAAAACGTCCAG
GAGGCCGCAGAAGAGGACGGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTGGAATCAAAGGATACAGACAGTGATAGGGAAGC
AGGGACTGAAACGGGGGGAGAGAACAATGATAAAGAAGAAGAAGAGAAGAAAGATGAAACTTCGAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCA
ATAAAGATGAAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCAATGTTTAGAGTCCTCATTGGCACTTACTATGACAATT
TCTGTGCCATTGCTAGGTTAATTGGGACCAAAACATGTAGACAGGTGTATGAGTTTAGAGTCAAAGAATCTAGCATCATAGCTCCAGCTCCCGCTGAGGA
TGTGGATACTCCTCCAAGGAAAAAGAAGAGGAAACACCGGTTGTGGGCTGCACACTGCAGAAAGATACAGCTGAAAAAGGACGGCTCCTCTAACCATGTT
TACAACTATCAACCCTGTGATCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGTGATAGCACAAAATTTTTGTGAAAAGTTTTGTCAATGTAGTT
CAGAGTGTCAAAACCGCTTTCCGGGATGCCGCTGCAAAGCACAGTGCAACACCAAGCAGTGCCCGTGCTACCTGGCTGTCCGAGAGTGTGACCCTGACCT
CTGTCTTACTTGTGGAGCCGCTGACCATTGGGACAGTAAAAATGTGTCCTGCAAGAACTGCAGTATTCAGCGGGGCTCCAAAAAGCATCTATTGCTGGCA
CCATCTGACGTGGCAGGCTGGGGGATTTTTATCAAAGATCCTGTGCAGAAAAATGAATTCATCTCAGAATACTGTGGAGAGATTATTTCTCAAGATGAAG
CTGACAGAAGAGGGAAAGTGTATGATAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGCAAGGGTAACAAAAT
TCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACAGGATAGGTATTTTTGCCAAGAGAGCCATCCAG
ACTGGCGAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGAAAGAGAAATGGAAATCCCTTGACATCTGCTAC
CTCCTCCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAGAAAAAGAACATGCAGTTTGAAATTCTGAATTTGC
AAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCTGCAAAGTGTTTTGTACCAGTGAATTTTTGCAA
TAATGCAGTATGGTACATTTTTCAACTTTGAATAAAGAATACTTGAACTTGTC
  • refseq_EZH2.F1 (forward primer)
  • refseq_EZH2.R1 (reverse primer)
  • Single exon skipping

Frame 0

QIKAMAIGLPRLALGPVASDTRWDSEGSGAPAA
AAAARGGDAREQRESARGTKNNHGPDWEEI*EGT
SLLAEACKIRVHATETAQEVQTS**SKEYV*FQ
SSENFGKNGNLKPRMETAKDTACAHPDFCELIA
RD*GGGR*NCFT*HSLYGR*SFRSGWYFH*RTNK
KL*WESTRG*RMWVYK**NFCGVGECPWSI***
*R***WRRS*RKRRKAERSGGSPR**RKPPTSE
ISF**NF*SHFLNVSR*GHSRRTKGKI*RTHRTA
APRRTSS*MYPQHRWTKC*ICSERAKLTLLSYA
FL*AMF*I*LLPTSFSCNTQHL*AEEHRNSSRQ
QTLWTTVLPAFGGSKGVCCCSHR*ADKDPTKTSR
RPQKRTASQ*Q*QAQHPHH*CAGIKGYRQ**GS
RD*NGGREQ**RRRREER*NFELL*SKFSVSNT
NKDEAKY*TS*ECGVEWC*SLNV*SPHWHLL*QF
LCHC*VNWDQNM*TGV*V*SQRI*HHSSSSR*G
CGYSSKEKEEETPVVGCTLQKDTAEKGRLL*PC
LQLSTL*SSTAAL*QFVPLCDSTKFL*KVLSM*F
RVSKPLSGMPLQSTVQHQAVPVLPGCPRV*P*P
LSYLWSR*PLGQ*KCVLQELQYSAGLQKASIAG
TI*RGRLGDFYQRSCAEK*IHLRILWRDYFSR*S
*QKRESV**IHVQLSVQLEQ*FCGGCNPQG*QN
SFCKSFGKSKLLCKSYDG*R*SQDRYFCQESHP
DWRRAVF*LQIQPG*CPEVCRHRKRNGNPLTSAT
SSPLL*NSCLSFRNLEYCGQFRKRTCSLKF*IC
KVL*E*FIVMSLKINFLLPSHQLQSVLYQ*IFA
IMQYGTFFNFE*RILELV

Frame 1

K*KRWRLGCRVWRSVRSRPTPGGTQKAVEPRRR
RRRRAGATRGNNASRRAGRRIIMGQTGKKSEKG
PVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSN
RQKILERTEILNQEWKQRRIQPVHILTSVSSLR
GTREVEDETVLHNIPYMGDEVLDQDGTFIEELI
KNYDGKVHGDRECGFINDEIFVELVNALGQYNDD
DDDDDGDDPEEREEKQKDLEDHRDDKESRPPRK
FPSDKIFEAISSMFPDKGTAEELKEKYKELTEQ
QLPGALPPECTPNIDGPNAKSVQREQSLHSFHTL
FCRRCFKYDCFLHPFHATPNTYKRKNTETALDN
KPCGPQCYQHLEGAKEFAAALTAERIKTPPKRP
GGRRRGRLPNNSSRPSTPTINVLESKDTDSDREA
GTETGGENNDKEEEEKKDETSSSSEANSRCQTP
IKMKPNIEPPENVEWSGAEASMFRVLIGTYYDN
FCAIARLIGTKTCRQVYEFRVKESSIIAPAPAED
VDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHV
YNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCS
SECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDL
CLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLA
PSDVAGWGIFIKDPVQKNEFISEYCGEIISQDE
ADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKI
RFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQ
TGEELFFDYRYSQADALKYVGIEREMEIP*HLL
PPPPSSETAALASGTSSTVGNLEKEHAV*NSEFA
KYCKNNL***V*KSTFYCLLTSCKVFCTSEFLQ
*CSMVHFSTLNKEYLNL

Frame 2

NKSDGDWAAAFGARSGRVRHPVGLRRQWSPGGG
GGGARGRRAGTTRVGARDEE*SWARLGRNLRRD
QFVGGSV*NQSTCD*DSSRGSDELMK*RVCLVP
IVRKFWKERKS*TKNGNSEGYSLCTS*LL*AHCA
GLGRWKMKLFYITFLIWEMKF*IRMVLSLKN**
KIMMGKYTGIENVGL*MMKFLWSW*MPLVNIMM
MTMMMMETILKKEKKSRKIWRITEMIKKAAHLGN
FLLIKFLKPFPQCFQIRAQQKN*RKNIKNSPNS
SSQAHFLLNVPPT*MDQMLNLFRESKAYTPFIR
FSVGDVLNMTASYILFMQHPTLISGRTQKQL*TT
NLVDHSVTSIWREQRSLLLLSPLSG*RPHQNVQ
EAAEEDGFPITVAGPAPPPLMCWNQRIQTVIGK
QGLKRGERTMIKKKKRRKMKLRAPLKQILGVKHQ
*R*SQILNLLRMWSGVVLKPQCLESSLALTMTI
SVPLLG*LGPKHVDRCMSLESKNLAS*LQLPLR
MWILLQGKRRGNTGCGLHTAERYS*KRTAPLTMF
TTINPVIIHGSLVTVRALV**HKIFVKSFVNVV
QSVKTAFRDAAAKHSATPSSARATWLSESVTLT
SVLLVEPLTIGTVKMCPARTAVFSGAPKSIYCWH
HLTWQAGGFLSKILCRKMNSSQNTVERLFLKMK
LTEEGKCMINTCAAFCST*TMILWWMQPARVTK
FVLQIIR*IQTAMQKL*WLTVITG*VFLPREPSR
LAKSCFLITDTARLMP*SMSASKEKWKSLDICY
LLPPPLKQLP*LQEPRVLWAI*KKNMQFEILNL
QSTVRIIYSNEFKNQLFIAFSPAAKCFVPVNFCN
NAVWYIFQL*IKNT*TC

Sequence NM_004456

CAAATAAAAGCGATGGCGATTGGGCTGCCGCGTTTGGCGCTCGGTCCGGTCGCGTCCGACACCCGGTGGGACTCAGAAGGCAGTGGAGCCCCGGCGGCGG
CGGCGGCGGCGCGCGGGGGCGACGCGCGGGAACAACGCGAGTCGGCGCGCGGGACGAAGAATAATCATGGGCCAGACTGGGAAGAAATCTGAGAAGGGAC
CAGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACATGCGACTGAGACAGCTCAAGAGGTTCAGACGAGCTGATGAAGTAAAGAGTATGTTTAGTTCCAA
TCGTCAGAAAATTTTGGAAAGAACGGAAATCTTAAACCAAGAATGGAAACAGCGAAGGATACAGCCTGTGCACATCCTGACTTCTGTGAGCTCATTGCGC
GGGACTAGGGAGTGTTCGGTGACCAGTGACTTGGATTTTCCAACACAAGTCATCCCATTAAAGACTCTGAATGCAGTTGCTTCAGTACCCATAATGTATT
CTTGGTCTCCCCTACAGCAGAATTTTATGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGAGATGAAGTTTTAGATCAGGATGGTACTTT
CATTGAAGAACTAATAAAAAATTATGATGGGAAAGTACACGGGGATAGAGAATGTGGGTTTATAAATGATGAAATTTTTGTGGAGTTGGTGAATGCCCTT
GGTCAATATAATGATGATGACGATGATGATGATGGAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCACCGAGATGATAAAGAAA
GCCGCCCACCTCGGAAATTTCCTTCTGATAAAATTTTTGAAGCCATTTCCTCAATGTTTCCAGATAAGGGCACAGCAGAAGAACTAAAGGAAAAATATAA
AGAACTCACCGAACAGCAGCTCCCAGGCGCACTTCCTCCTGAATGTACCCCCAACATAGATGGACCAAATGCTAAATCTGTTCAGAGAGAGCAAAGCTTA
CACTCCTTTCATACGCTTTTCTGTAGGCGATGTTTTAAATATGACTGCTTCCTACATCGTAAGTGCAATTATTCTTTTCATGCAACACCCAACACTTATA
AGCGGAAGAACACAGAAACAGCTCTAGACAACAAACCTTGTGGACCACAGTGTTACCAGCATTTGGAGGGAGCAAAGGAGTTTGCTGCTGCTCTCACCGC
TGAGCGGATAAAGACCCCACCAAAACGTCCAGGAGGCCGCAGAAGAGGACGGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG
GAATCAAAGGATACAGACAGTGATAGGGAAGCAGGGACTGAAACGGGGGGAGAGAACAATGATAAAGAAGAAGAAGAGAAGAAAGATGAAACTTCGAGCT
CCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAGATGAAGCCAAATATTGAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCAATGTT
TAGAGTCCTCATTGGCACTTACTATGACAATTTCTGTGCCATTGCTAGGTTAATTGGGACCAAAACATGTAGACAGGTGTATGAGTTTAGAGTCAAAGAA
TCTAGCATCATAGCTCCAGCTCCCGCTGAGGATGTGGATACTCCTCCAAGGAAAAAGAAGAGGAAACACCGGTTGTGGGCTGCACACTGCAGAAAGATAC
AGCTGAAAAAGGACGGCTCCTCTAACCATGTTTACAACTATCAACCCTGTGATCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGTGATAGCACA
AAATTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCGGGATGCCGCTGCAAAGCACAGTGCAACACCAAGCAGTGCCCGTGC
TACCTGGCTGTCCGAGAGTGTGACCCTGACCTCTGTCTTACTTGTGGAGCCGCTGACCATTGGGACAGTAAAAATGTGTCCTGCAAGAACTGCAGTATTC
AGCGGGGCTCCAAAAAGCATCTATTGCTGGCACCATCTGACGTGGCAGGCTGGGGGATTTTTATCAAAGATCCTGTGCAGAAAAATGAATTCATCTCAGA
ATACTGTGGAGAGATTATTTCTCAAGATGAAGCTGACAGAAGAGGGAAAGTGTATGATAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTT
GTGGTGGATGCAACCCGCAAGGGTAACAAAATTCGTTTTGCAAATCATTCGGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAACGGTGATCACA
GGATAGGTATTTTTGCCAAGAGAGCCATCCAGACTGGCGAAGAGCTGTTTTTTGATTACAGATACAGCCAGGCTGATGCCCTGAAGTATGTCGGCATCGA
AAGAGAAATGGAAATCCCTTGACATCTGCTACCTCCTCCCCCCTCCTCTGAAACAGCTGCCTTAGCTTCAGGAACCTCGAGTACTGTGGGCAATTTAGAA
AAAGAACATGCAGTTTGAAATTCTGAATTTGCAAAGTACTGTAAGAATAATTTATAGTAATGAGTTTAAAAATCAACTTTTTATTGCCTTCTCACCAGCT
GCAAAGTGTTTTGTACCAGTGAATTTTTGCAATAATGCAGTATGGTACATTTTTCAACTTTGAATAAAGAATACTTGAACTTGTC
  • refseq_EZH2.F1 (forward primer)
  • refseq_EZH2.R1 (reverse primer)
  • Single exon skipping

Frame 0

QIKAMAIGLPRLALGPVASDTRWDSEGSGAPAA
AAAARGGDAREQRESARGTKNNHGPDWEEI*EGT
SLLAEACKIRVHATETAQEVQTS**SKEYV*FQ
SSENFGKNGNLKPRMETAKDTACAHPDFCELIA
RD*GVFGDQ*LGFSNTSHPIKDSECSCFSTHNVF
LVSPTAEFYGGR*NCFT*HSLYGR*SFRSGWYF
H*RTNKKL*WESTRG*RMWVYK**NFCGVGECP
WSI****R***WRRS*RKRRKAERSGGSPR**RK
PPTSEISF**NF*SHFLNVSR*GHSRRTKGKI*
RTHRTAAPRRTSS*MYPQHRWTKC*ICSERAKL
TLLSYAFL*AMF*I*LLPTS*VQLFFSCNTQHL*
AEEHRNSSRQQTLWTTVLPAFGGSKGVCCCSHR
*ADKDPTKTSRRPQKRTASQ*Q*QAQHPHH*CA
GIKGYRQ**GSRD*NGGREQ**RRRREER*NFEL
L*SKFSVSNTNKDEAKY*TS*ECGVEWC*SLNV
*SPHWHLL*QFLCHC*VNWDQNM*TGV*V*SQR
I*HHSSSSR*GCGYSSKEKEEETPVVGCTLQKDT
AEKGRLL*PCLQLSTL*SSTAAL*QFVPLCDST
KFL*KVLSM*FRVSKPLSGMPLQSTVQHQAVPV
LPGCPRV*P*PLSYLWSR*PLGQ*KCVLQELQYS
AGLQKASIAGTI*RGRLGDFYQRSCAEK*IHLR
ILWRDYFSR*S*QKRESV**IHVQLSVQLEQ*F
CGGCNPQG*QNSFCKSFGKSKLLCKSYDG*R*SQ
DRYFCQESHPDWRRAVF*LQIQPG*CPEVCRHR
KRNGNPLTSATSSPLL*NSCLSFRNLEYCGQFR
KRTCSLKF*ICKVL*E*FIVMSLKINFLLPSHQL
QSVLYQ*IFAIMQYGTFFNFE*RILELV

Frame 1

K*KRWRLGCRVWRSVRSRPTPGGTQKAVEPRRR
RRRRAGATRGNNASRRAGRRIIMGQTGKKSEKG
PVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSN
RQKILERTEILNQEWKQRRIQPVHILTSVSSLR
GTRECSVTSDLDFPTQVIPLKTLNAVASVPIMY
SWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTF
IEELIKNYDGKVHGDRECGFINDEIFVELVNAL
GQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKE
SRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYK
ELTEQQLPGALPPECTPNIDGPNAKSVQREQSL
HSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTY
KRKNTETALDNKPCGPQCYQHLEGAKEFAAALTA
ERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVL
ESKDTDSDREAGTETGGENNDKEEEEKKDETSS
SSEANSRCQTPIKMKPNIEPPENVEWSGAEASMF
RVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKE
SSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKI
QLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQ
NFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPC
YLAVRECDPDLCLTCGAADHWDSKNVSCKNCSI
QRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISE
YCGEIISQDEADRRGKVYDKYMCSFLFNLNNDF
VVDATRKGNKIRFANHSVNPNCYAKVMMVNGDH
RIGIFAKRAIQTGEELFFDYRYSQADALKYVGIE
REMEIP*HLLPPPPSSETAALASGTSSTVGNLE
KEHAV*NSEFAKYCKNNL***V*KSTFYCLLTS
CKVFCTSEFLQ*CSMVHFSTLNKEYLNL

Frame 2

NKSDGDWAAAFGARSGRVRHPVGLRRQWSPGGG
GGGARGRRAGTTRVGARDEE*SWARLGRNLRRD
QFVGGSV*NQSTCD*DSSRGSDELMK*RVCLVP
IVRKFWKERKS*TKNGNSEGYSLCTS*LL*AHCA
GLGSVR*PVTWIFQHKSSH*RL*MQLLQYP*CI
LGLPYSRILWWKMKLFYITFLIWEMKF*IRMVL
SLKN**KIMMGKYTGIENVGL*MMKFLWSW*MPL
VNIMMMTMMMMETILKKEKKSRKIWRITEMIKK
AAHLGNFLLIKFLKPFPQCFQIRAQQKN*RKNI
KNSPNSSSQAHFLLNVPPT*MDQMLNLFRESKAY
TPFIRFSVGDVLNMTASYIVSAIILFMQHPTLI
SGRTQKQL*TTNLVDHSVTSIWREQRSLLLLSP
LSG*RPHQNVQEAAEEDGFPITVAGPAPPPLMCW
NQRIQTVIGKQGLKRGERTMIKKKKRRKMKLRA
PLKQILGVKHQ*R*SQILNLLRMWSGVVLKPQC
LESSLALTMTISVPLLG*LGPKHVDRCMSLESKN
LAS*LQLPLRMWILLQGKRRGNTGCGLHTAERY
S*KRTAPLTMFTTINPVIIHGSLVTVRALV**H
KIFVKSFVNVVQSVKTAFRDAAAKHSATPSSARA
TWLSESVTLTSVLLVEPLTIGTVKMCPARTAVF
SGAPKSIYCWHHLTWQAGGFLSKILCRKMNSSQ
NTVERLFLKMKLTEEGKCMINTCAAFCST*TMIL
WWMQPARVTKFVLQIIR*IQTAMQKL*WLTVIT
G*VFLPREPSRLAKSCFLITDTARLMP*SMSAS
KEKWKSLDICYLLPPPLKQLP*LQEPRVLWAI*K
KNMQFEILNLQSTVRIIYSNEFKNQLFIAFSPA
AKCFVPVNFCNNAVWYIFQL*IKNT*TC

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