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Sequences

Sequence NM_000793

GGCTGCAGAGAGAGGCACTTTGCACCACAGACAGATAGCAAGAAGGGAAAGACAGAGAGTGAGAAAAAAGAGGAGTCAGTCGCTCCTGGGGAAGGGAGAG AGTGAGACTGGGAGAAAGAGAAGCACAGAAAGTGTGTGTAAAACGGAGTAAAGAAAGAAAAAAAAAAAACTACCCTTAAAGCACATTTAAAAAAAAAAAA AACTCTGGCAATTCAAGAAAGAAACAGGCTACGTTTAAAGAGCATAGAGACAATGAAAGGCTAAAGAAAATTTTAAAATCTCTGCCACAGTCTCATAGGT GCTTGGAAATGAAAGTAGAACTGCCTGTCTTTAACGGACTCTGACAGAGAGGGTGAAGGGGAACCAGAGCGCACAAGGGAACTGACTCAGGAGGCAGAGA AGATGGGCATCCTCAGCGTAGACTTGCTGATCACACTGCAAATTCTGCCAGTTTTTTTCTCCAACTGCCTCTTCCTGGCTCTCTATGACTCGGTCATTCT GCTCAAGCACGTGGTGCTGCTGTTGAGCCGCTCCAAGTCCACTCGCGGAGAGTGGCGGCGCATGCTGACCTCAGAGGGACTGCGCTGCGTCTGGAAGAGC TTCCTCCTCGATGCCTACAAACAGGTGAAATTGGGTGAGGATGCCCCCAATTCCAGTGTGGTGCATGTCTCCAGTACAGAAGGAGGTGACAACAGTGGCA ATGGTACCCAGGAGAAGATAGCTGAGGGAGCCACATGCCACCTTCTTGACTTTGCCAGCCCTGAGCGCCCACTAGTGGTCAACTTTGGCTCAGCCACTTG ACCTCCTTTCACGAGCCAGCTGCCAGCCTTCCGCAAACTGGTGGAAGAGTTCTCCTCAGTGGCTGACTTCCTGCTGGTCTACATTGATGAGGCTCATCCA TCAGATGGCTGGGCGATACCGGGGGACTCCTCTTTGTCTTTTGAGGTGAAGAAGCACCAGAACCAGGAAGATCGATGTGCAGCAGCCCAGCAGCTTCTGG AGCGTTTCTCCTTGCCGCCCCAGTGCCGAGTTGTGGCTGACCGCATGGACAATAACGCCAACATAGCTTACGGGGTAGCCTTTGAACGTGTGTGCATTGT GCAGAGACAGAAAATTGCTTATCTGGGAGGAAAGGGCCCCTTCTCCTACAACCTTCAAGAAGTCCGGCATTGGCTGGAGAAGAATTTCAGCAAGAGATGA AAGAAAACTAGATTAGCTGGTTAAAGGTATGATTATAAGAGAGCTTATTGTTTTAAAAAGTTATATAAAGGCAAGGAAATTAAGAACTGAATCCATATTT CAACAGAGCCCTATTGGCTTACTGAAAGACAGGAGTTTATCTATCGGAAGAACATGAATCTCTAACAGCTCCATACTTCTTTCACTACTCAAATGGCATT GGGCTGAGTAAGTAACCATATCACCTCTCTTCTTAGTAAAAAGCCCTATGTGAAAAGATCCCAAGATGGAGAGGAAGAAACGCTAATTCAGCATGTGTTC ATTCTGCATTGAGAAGGAACTGATACATCTGATGCATGCTTTGAGACCAGAAGAAAAGACTTACCTGAATAATTACTACATTAGGGAAGCTACTGTCTAC GTTAAGATAAAGGGTATTGCCTTGGCTCTATTTGGCATGGATGGAGCCCAGTTGGAAAATTCCCAAATATTACAACAAGTCCTTGAACCCAGGCCATGTG GTTAGACGTTGGTGTTAAGGTTAGACCTTATGTTAGAGTCATTTCTGATGTTCCAGCTTCTAGCCATGTAGTGCTCTCAGTCTTCATACCCCAGAAATTA TTGGTATATTTGTAGATACCGAGAATGATCCCTCAGTCTGAGAGGTTAGAATGATCATCTGTAATCTGAGGGTTAATTTCTAGGCAGGTGGAGAGAGTGG TAAAAAAGAAATGAAATTGACAAGCTAGGAAAGAGGAGGCAGAAAGATTTGGAAAATTCACAGAGTTTCACCCTTAAGCTGTAGAGAGTGGGTCACATTT GTTAGCCACGGAAACATAGAAACATACACAAGGCCAGAAAAAGAAGAAGGAGCTCAACTAAAAGTGGCATAGAGAATACACATATAAAAACAATATATTT GTCATATGCTCCTAGAGAGGAGAAAGGGGTGATTGAAAGAAAAAAAAATACTTAAATATTTGTAATTGTGAGGGGTTTCTTTTGGAAATAATTACTTTTG AACCATGTATGTGGTATGTATATTTTCAGTGGGTTAATTATACCCCATGATACCTATTAAAGGAAAACCAGTGGGTCTGGTGGTGCTGGTCTTTTCCTCC CCATTCCTACAATTTCTATGTGGCCCAAGTCATTCCTAATCTTGGTCTCTATAGCAGTGTTCTCTCTGAATGCTGAGCTGAAGAAATTATACGTACATAC ACACATACATACATACATACAAATATATGTATATATATTCTCAGCTGCTGCGGGAGGTAGGTACCATGGCCATTCAGCACAGCCTTGATTTCCTCCCAAA GTAGGTGAGCTATAGTGAAGAATAGGTGCAAACAAACAAGCTTACTTCCATTGCAAAATAGAAGAAGAGGAAGTTAGAGATAATTCTGATCAATCATTTT GGAGGCTTTGTTATAAGGCAACCCCCGGTATATCATGGAATTTCCATTGACATTTGAATTTGGACTTGGATCTTCCCTTGGTCCCATTAGCTGAGGTTTA GTAATCTAAAGTCCCTATAGTATATGATTATAATGCTATTTTAAAAAATATATATATAAAATATTTTTTTCTTTTTAAAATAGACACTATAGTTTTACCC ATAAGTAATATTTAAAGATTATAGCTCCCAAAAGAATGGACCAACCACTTTCGTATCATAATTTCTTTTTGGTAAATATGAGACTATTATGAAATCATAG TATATGATTGTATTTAAAGGTACAATCAAAGGATCTTTTGTCCATTCCATTAATAACTGAATAAAAAATAAATAAAATGGATAGAAAAAAACTAAAGTTG AAAATACATTCTTAAACTAGTTGTCTGAATTGAGAAAAGAGTGAGAACTAGGTGTGCAAGAACCAAACGTATTTTATTTTATTTTTTAAATGGGAGCAAC ATATCAGTCGTGTCACCAGCTGGTATATTGTGTAAATATTAAAGCTCCATTGGGACTGATTTTTCATGGCAACATCAGCTTTCTAATGTTCTAAATTCTA TAAAAACCACCCACAAAGAAACAAAGCAAATTTCATTATCTAATGAGTTGCTGGAAAATCATATTGAGAATAATTATTTCAGATTCCTCAGTTGTTAACT TCTACATTCAAGGCTTATCTCTGCCCCCATTGATTTTTAACCTCAAAATGGTGTTGAGATTTACGTGGAACCCTAAAGCAGTAAAATAAAAAACCTGGTT GCAGCACATTCACACTGTTGTCCTTAAAATTCCCCTTTTTTCTCTATGTACGATAAAGTAACAGTATGTCAGATAAGCCGGTGGGGGGATGAGATTAGGC TGAGGCAGTGCTAGTCAACTGGGGAAAAGGATGATGGAAAAATCACCCAGTTGTGCTATATTTTTAAAGAAGGAGGTCGTTTATGTGTGCAGACAATTCT CCCTGAGGTTAGCCCAATGGAGAAATGAAGCAGAGGAAGGAAACATAGAAAGACATGGGCTATCAGGGAGGAAGATGTTCAATAGAACATGCAAGAATTT CTGGAAGAAAGGCTGTGGAAGGGCCAATGGAGAAAATGAATGGACAAAGCTCAGGAATCCTACGCTATGTAGAATGTCTTGGTGTTATCAGGGTTAAGCC TGTAATTATGTAACCTATTTATCGCAACATGAATTTTTATGATTTCTTGTGATGTATTCTTTTATGAAATTAACAAGAACTCATTATTTTGAGGTAGAGG AAAATCAATGCTTTATCTGATATGCTGAGAAATTATTAGATTGCCAATACTCATGTGCGTTTCATGTGTTTTATAAGGTTTGTTCCTTTGAAGAATTGTA GTTCTTAGTCCCACAGGGAAATGTGTATCTATTTATATATCATAGTATAAATCTATGATATATTTATATCATATATAAAAGTCTGAGTTCTCTTTCTTAG TCCCTAATCATGTTTCTCCCATAGGCTGTGTTTACATGGAGCTATCGGTTTAGCCTTTTAAGCTTCATTAGCTTGTCTATTATTGAAATAGTTTCCAAGA AATTTTAGATATTATCATAACATCTGGGTCTACTCAAACACTTATTGTTTGAAAGACTTATGTCTTGGACCTATCAAAAACTGACTTTATTTATTGCTTA GTGAAAATACTAGTGGGATCAACAATGATTTTCTTGAATGGGCATGAATGGAGATGCCCGCACAGTAATGTAGAAATGTTTCATACAGCTATTAAAATGT AACTGACCTCCTTAGAGGCAGATTAGTAACTGTTCCTACTTTGTATAGCTAAGTGACAGTCACTTAACTTACATGACTTTCTTTTTTCACATTGGGTCTC TGGTCCTGTGTCTTCACCTCATTTATAGCACGTCTCCTTGATTTTTGGTAGTATCAACTTCCCAGTGATCTGTTCAGTTAAGTTCTTCTCCCGTTAACCA GGAAGTGCTTATTCTCTCATCACAGTGGGAAGAATAGCCTATTGTCTTTCATTTTGCCTGAGTGTATTTTACTATTTGGGCTCTGAAATAAAAATTATGA AATATGGTGAGGTCACATGTTGGTGCTGCCTTGCTGCATAAAATTCTAGAGGGCAGGTTAGAGACAGTATGTATGCCTTCGGGAAAATTCAAAGGTGGAT TACAAGGTGTCCTCAGCATGCCCTATGGCCTATGTGCGAAGCAAGAAGAATTGACTGATTTACAGGACTTCTCTTTATGTCAATCTTAAGAGGATGGATG AATCTGGACATTTGTTCCACCCGACCTCTGACTGATGGTTTGGAAAATAACTTTAATTAGGATCATATGACCATTGAAAAAGGAAAAATGTAGACTCTGA CTTCCGTCCCACTGAAGGATTAATGAAAACCTTTACTAGCATTTAGAGCTTTTCAGAACATCCCCACTGTCATGTGTCTCAGCAGTGGAGACTGCAAGTA AGGCTTTTAATTTTAGGAGGTTTTTTTTTTTTTTTTTTTTTCCCTAAATGGTATGGCCAAAAGTCAGAGTTAAAATATATATAGTTAGATTCCAACTTCC TCCTTCACTCTAAAAATAGAATCCAAACCCACTCTTCATATATGCTTCCAGAATGGGGCTTAAGTACCAATCTCTGCTTTGCAATGGGCACAATCTTGGT CATGTCCTGAGGCTCTCTAAGAAAAGAGAGGATCTAGGATGGGAGAGCTAGAAAGTTGCTAACTGGGAAGAACAAGGCCCTGAGGGCTTGGTCTACCAAT CTGGGAAGATTTGAAAACAAACTTCTCGCAACTGAAGGAAGGCTGAAGGCTGCTGCAAGTCATTGAGTGACTTTAGGATGAGCAAAACATTGGGCCACTT CCTAATGCCCTATGTGTATAGTACCAGAAGCAAGGTCTCAGACTTAACAGACCCAGCTCTGTTCCAAGGTGAGTCTGAACCAATAGAAAGCAAACATGTG CAGATATCCAAACAAGACTGCTCATGCAAGTCGGGGCTGGCTACCCGTCTTAGGCAGCAACAGCAGAGCTCCAGGGAGCTTATTCAATATTTACTGAGAC TTCGAAGACCCAGCAGATGTTTAATGAAGTCACTATTTTGGCTCAAACCCTCCACTTCTCCCCCTCCCCTCAAAAAGCCAACAGGTAAACACATAAATGA AAGAAACCCACAGAAGGGGATGGGAAATAAAGAAAATTCTCTCAAGACTTCTCCAGGCCCATGTCACTGGTCAGCGTGGTTTTTATGTGTATTAGGATTG GGGGATGTGAAGAAATAAGTATCCAGTACTTTATAACCAAAGCAATTAAATGATATTGGGTAGGGAATGTTGGCCAGTTTTGTTTAGTTTTGCATCACAT TGTCACCAGACTCACTAGCCCAAGTAATCGGGCGCCCGAAGAGGGAGACAGAGATGTGCAGAGTTGACCAGTGTGCGGATGATAACTACTGACGAAAGAG TCATCGACTCAGTTAGTGGTTGGATGTAGTCACATTAGTTTGCCTCTCCCCATCTTTGTCTCCCTGGCAAGGAGAATATGCGGGACATGATGCTAAGAGT CCTGGGTAAATGTGGTGAGAATGCACGCGTGCATATGCTACACATATGTGCTTCTCAGTTGCAGAAAATGAACTGCTTTGGGAGATTATCAGTAGAAAGA GTGTTATCATATTGGTGCTGAGTGCTATGTGTGCTTATACAATTTGTTCTTGTATTTTAATAAACTTTGAATAAAAGAATAAAGCTA

refseq_DIO2.F1 (forward primer)
refseq_DIO2.R1 (reverse primer)
Single exon skipping

Frame 0

GCRERHFAPQTDSKKGKTESEKKEESVAPGEGR E*DWEKEKHRKCV*NGVKKEKKKTTLKAHLKKKK TLAIQERNRLRLKSIETMKG*RKF*NLCHSLIG AWK*K*NCLSLTDSDREGEGEPERTRELTQEAE KMGILSVDLLITLQILPVFFSNCLFLALYDSVIL LKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKS FLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSG NGTQEKIAEGATCHLLDFASPERPLVVNFGSAT* PPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHP SDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLL ERFSLPPQCRVVADRMDNNANIAYGVAFERVCIV QRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKR* KKTRLAG*RYDYKRAYCFKKLYKGKEIKN*IHI STEPYWLTERQEFIYRKNMNL*QLHTSFTTQMAL G*VSNHITSLLSKKPYVKRSQDGEEETLIQHVF ILH*EGTDTSDACFETRRKDLPE*LLH*GSYCL R*DKGYCLGSIWHGWSPVGKFPNITTSP*TQAMW LDVGVKVRPYVRVISDVPASSHVVLSVFIPQKL LVYL*IPRMIPQSERLE*SSVI*GLISRQVERV VKKK*N*QARKEEAERFGKFTEFHP*AVESGSHL LATET*KHTQGQKKKKELN*KWHREYTYKNNIF VICS*RGERGD*KKKKYLNICNCEGFLLEIITF EPCMWYVYFQWVNYTP*YLLKENQWVWWCWSFPP HSYNFYVAQVIPNLGLYSSVLSEC*AEEIIRTY THTYIHTNICIYILSCCGR*VPWPFSTALISSQ SR*AIVKNRCKQTSLLPLQNRRRGS*R*F*SIIL EALL*GNPRYIMEFPLTFEFGLGSSLGPIS*GL VI*SPYSI*L*CYFKKYIYKIFFSF*NRHYSFT HK*YLKIIAPKRMDQPLSYHNFFLVNMRLL*NHS I*LYLKVQSKDLLSIPLITE*KINKMDRKKLKL KIHS*TSCLN*EKSEN*VCKNQTYFILFFKWEQ HISRVTSWYIV*ILKLHWD*FFMATSAF*CSKFY KNHPQRNKANFII**VAGKSY*E*LFQIPQLLT STFKAYLCPH*FLTSKWC*DLRGTLKQ*NKKPG CSTFTLLSLKFPFFLYVR*SNSMSDKPVGG*D*A EAVLVNWGKG*WKNHPVVLYF*RRRSFMCADNS P*G*PNGEMKQRKET*KDMGYQGGRCSIEHARI SGRKAVEGPMEKMNGQSSGILRYVECLGVIRVKP VIM*PIYRNMNFYDFL*CILL*N*QELIILR*R KINALSDMLRNY*IANTHVRFMCFIRFVPLKNC SS*SHREMCIYLYIIV*IYDIFISYIKV*VLFLS P*SCFSHRLCLHGAIGLAF*ASLACLLLK*FPR NFRYYHNIWVYSNTYCLKDLCLGPIKN*LYLLL SENTSGINNDFLEWA*MEMPAQ*CRNVSYSY*NV TDLLRGRLVTVPTLYS*VTVT*LT*LSFFTLGL WSCVFTSFIARLLDFW*YQLPSDLFS*VLLPLT RKCLFSHHSGKNSLLSFILPECILLFGL*NKNYE IW*GHMLVLPCCIKF*RAG*RQYVCLRENSKVD YKVSSACPMAYVRSKKN*LIYRTSLYVNLKRMD ESGHLFHPTSD*WFGK*L*LGSYDH*KRKNVDSD FRPTEGLMKTFTSI*SFSEHPHCHVSQQWRLQV RLLILGGFFFFFFFPKWYGQKSELKYI*LDSNF LLHSKNRIQTHSSYMLPEWGLSTNLCFAMGTILV MS*GSLRKERI*DGRARKLLTGKNKALRAWSTN LGRFENKLLATEGRLKAAASH*VTLG*AKHWAT S*CPMCIVPEARSQT*QTQLCSKVSLNQ*KANMC RYPNKTAHASRGWLPVLGSNSRAPGSLFNIY*D FEDPADV**SHYFGSNPPLLPLPSKSQQVNT*M KETHRRGWEIKKILSRLLQAHVTGQRGFYVY*DW GM*RNKYPVLYNQSN*MILGRECWPVLFSFASH CHQTH*PK*SGARRGRQRCAELTSVRMITTDER VIDSVSGWM*SH*FASPHLCLPGKENMRDMMLRV LGKCGENARVHMLHICASQLQKMNCFGRLSVER VLSYWC*VLCVLIQFVLVF**TLNKRIKL

Frame 1

AAERGTLHHRQIARRERQRVRKKRSQSLLGKGE SETGRKRSTESVCKTE*RKKKKKLPLKHI*KKK KLWQFKKETGYV*RA*RQ*KAKENFKISATVS*V LGNESRTACL*RTLTERVKGNQSAQGN*LRRQR RWASSA*TC*SHCKFCQFFSPTASSWLSMTRSF CSSTWCCC*AAPSPLAESGGAC*PQRDCAASGRA SSSMPTNR*NWVRMPPIPVWCMSPVQKEVTTVA MVPRRR*LREPHATFLTLPALSAH*WSTLAQPL DLLSRASCQPSANWWKSSPQWLTSCWSTLMRLIH QMAGRYRGTPLCLLR*RSTRTRKIDVQQPSSFW SVSPCRPSAELWLTAWTITPT*LTG*PLNVCAL CRDRKLLIWEERAPSPTTFKKSGIGWRRISARDE RKLD*LVKGMIIRELIVLKSYIKARKLRTESIF QQSPIGLLKDRSLSIGRT*ISNSSILLSLLKWH WAE*VTISPLFLVKSPM*KDPKMERKKR*FSMCS FCIEKELIHLMHALRPEEKTYLNNYYIREATVY VKIKGIALALFGMDGAQLENSQILQQVLEPRPC G*TLVLRLDLMLESFLMFQLLAM*CSQSSYPRNY WYICRYRE*SLSLRG*NDHL*SEG*FLGRWREW *KRNEIDKLGKRRQKDLENSQSFTLKL*RVGHI C*PRKHRNIHKARKRRRSSTKSGIENTHIKTIYL SYAPREEKGVIERKKNT*IFVIVRGFFWK*LLL NHVCGMYIFSGLIIPHDTY*RKTSGSGGAGLFL PIPTISMWPKSFLILVSIAVFSLNAELKKLYVHT HIHTYIQIYVYIFSAAAGGRYHGHSAQP*FPPK VGEL**RIGANKQAYFHCKIEEEEVRDNSDQSF WRLCYKATPGISWNFH*HLNLDLDLPLVPLAEV* *SKVPIVYDYNAILKNIYIKYFFLFKIDTIVLP ISNI*RL*LPKEWTNHFRIIISFW*I*DYYEII VYDCI*RYNQRIFCPFH**LNKK*IKWIEKN*S* KYILKLVV*IEKRVRTRCARTKRILFYFLNGSN ISVVSPAGILCKY*SSIGTDFSWQHQLSNVLNS IKTTHKETKQISLSNELLENHIENNYFRFLSC*L LHSRLISAPIDF*PQNGVEIYVEP*SSKIKNLV AAHSHCCP*NSPFFSMYDKVTVCQISRWGDEIR LRQC*STGEKDDGKITQLCYIFKEGGRLCVQTIL PEVSPMEK*SRGRKHRKTWAIREEDVQ*NMQEF LEERLWKGQWRK*MDKAQESYAM*NVLVLSGLS L*LCNLFIAT*IFMISCDVFFYEINKNSLF*GRG KSMLYLIC*EIIRLPILMCVSCVL*GLFL*RIV VLSPTGKCVSIYIS*YKSMIYLYHI*KSEFSFL VPNHVSPIGCVYMELSV*PFKLH*LVYY*NSFQE ILDIIITSGSTQTLIV*KTYVLDLSKTDFIYCL VKILVGSTMIFLNGHEWRCPHSNVEMFHTAIKM *LTSLEAD**LFLLCIAK*QSLNLHDFLFSHWVS GPVSSPHL*HVSLIFGSINFPVICSVKFFSR*P GSAYSLITVGRIAYCLSFCLSVFYYLGSEIKIM KYGEVTCWCCLAA*NSRGQVRDSMYAFGKIQRWI TRCPQHALWPMCEARRID*FTGLLFMSILRGWM NLDICSTRPLTDGLENNFN*DHMTIEKGKM*TL TSVPLKD**KPLLAFRAFQNIPTVMCLSSGDCK* GF*F*EVFFFFFFSLNGMAKSQS*NIYS*IPTS SFTLKIESKPTLHICFQNGA*VPISALQWAQSW SCPEAL*EKRGSRMGELESC*LGRTRP*GLGLPI WEDLKTNFSQLKEG*RLLQVIE*L*DEQNIGPL PNALCV*YQKQGLRLNRPSSVPR*V*TNRKQTC ADIQTRLLMQVGAGYPS*AATAELQGAYSIFTET SKTQQMFNEVTILAQTLHFSPSPQKANR*THK* KKPTEGDGK*RKFSQDFSRPMSLVSVVFMCIRI GGCEEISIQYFITKAIK*YWVGNVGQFCLVLHHI VTRLTSPSNRAPEEGDRDVQS*PVCG**LLTKE SSTQLVVGCSHISLPLPIFVSLARRICGT*C*E SWVNVVRMHACICYTYVLLSCRK*TALGDYQ*KE CYHIGAECYVCLYNLFLYFNKL*IKE*S

Frame 2

LQREALCTTDR*QEGKDRE*EKRGVSRSWGRER VRLGEREAQKVCVKRSKERKKKNYP*STFKKKK NSGNSRKKQATFKEHRDNERLKKILKSLPQSHR CLEMKVELPVFNGL*QRG*RGTRAHKGTDSGGRE DGHPQRRLADHTANSASFFLQLPLPGSL*LGHS AQARGAAVEPLQVHSRRVAAHADLRGTALRLEE LPPRCLQTGEIG*GCPQFQCGACLQYRRR*QQWQ WYPGEDS*GSHMPPS*LCQP*APTSGQLWLSHL TSFHEPAASLPQTGGRVLLSG*LPAGLH**GSS IRWLGDTGGLLFVF*GEEAPEPGRSMCSSPAASG AFLLAAPVPSCG*PHGQ*RQHSLRGSL*TCVHC AETENCLSGRKGPLLLQPSRSPALAGEEFQQEM KEN*ISWLKV*L*ESLLF*KVI*RQGN*ELNPYF NRALLAY*KTGVYLSEEHESLTAPYFFHYSNGI GLSK*PYHLSS**KALCEKIPRWRGRNANSACV HSALRRN*YI*CML*DQKKRLT*IITTLGKLLST LR*RVLPWLYLAWMEPSWKIPKYYNKSLNPGHV VRRWC*G*TLC*SHF*CSSF*PCSALSLHTPEI IGIFVDTENDPSV*EVRMIICNLRVNF*AGGESG KKEMKLTS*ERGGRKIWKIHRVSPLSCREWVTF VSHGNIETYTRPEKEEGAQLKVA*RIHI*KQYI CHMLLERRKG*LKEKKILKYL*L*GVSFGNNYF* TMYVVCIFSVG*LYPMIPIKGKPVGLVVLVFSS PFLQFLCGPSHS*SWSL*QCSL*MLS*RNYTYI HTYIHTYKYMYIYSQLLREVGTMAIQHSLDFLPK *VSYSEE*VQTNKLTSIAK*KKRKLEIILINHF GGFVIRQPPVYHGISIDI*IWTWIFPWSH*LRF SNLKSL*YMIIMLF*KIYI*NIFFFLK*TL*FYP *VIFKDYSSQKNGPTTFVS*FLFGKYETIMKS* YMIVFKGTIKGSFVHSINN*IKNK*NG*KKTKV ENTFLN*LSELRKE*ELGVQEPNVFYFIF*MGAT YQSCHQLVYCVNIKAPLGLIFHGNISFLMF*IL *KPPTKKQSKFHYLMSCWKIILRIIISDSSVVN FYIQGLSLPPLIFNLKMVLRFTWNPKAVK*KTWL QHIHTVVLKIPLFSLCTIK*QYVR*AGGGMRLG *GSASQLGKRMMEKSPSCAIFLKKEVVYVCRQF SLRLAQWRNEAEEGNIERHGLSGRKMFNRTCKNF WKKGCGRANGENEWTKLRNPTLCRMSWCYQG*A CNYVTYLSQHEFL*FLVMYSFMKLTRTHYFEVE ENQCFI*YAEKLLDCQYSCAFHVFYKVCSFEEL* FLVPQGNVYLFIYHSINL*YIYIIYKSLSSLS* SLIMFLP*AVFTWSYRFSLLSFISLSIIEIVSK KF*ILS*HLGLLKHLLFERLMSWTYQKLTLFIA* *KY*WDQQ*FS*MGMNGDARTVM*KCFIQLLKC N*PP*RQISNCSYFV*LSDSHLTYMTFFFHIGS LVLCLHLIYSTSP*FLVVSTSQ*SVQLSSSPVNQ EVLILSSQWEE*PIVFHFA*VYFTIWALK*KL* NMVRSHVGAALLHKILEGRLETVCMPSGKFKGG LQGVLSMPYGLCAKQEELTDLQDFSLCQS*EDG* IWTFVPPDL*LMVWKITLIRII*PLKKEKCRL* LPSH*RINENLY*HLELFRTSPLSCVSAVETAS KAFNFRRFFFFFFFP*MVWPKVRVKIYIVRFQLP PSL*K*NPNPLFIYASRMGLKYQSLLCNGHNLG HVLRLSKKREDLGWES*KVANWEEQGPEGLVYQ SGKI*KQTSRN*RKAEGCCKSLSDFRMSKTLGHF LMPYVYSTRSKVSDLTDPALFQGESEPIESKHV QISKQDCSCKSGLATRLRQQQQSSRELIQYLLR LRRPSRCLMKSLFWLKPSTSPPPLKKPTGKHINE RNPQKGMGNKENSLKTSPGPCHWSAWFLCVLGL GDVKK*VSSTL*PKQLNDIG*GMLASFV*FCIT LSPDSLAQVIGRPKRETEMCRVDQCADDNY*RKS HRLS*WLDVVTLVCLSPSLSPWQGEYAGHDAKS PG*MW*ECTRAYATHMCFSVAENELLWEIISRK SVIILVLSAMCAYTICSCILINFE*KNKA

Sequence NM_013989

GGCTGCAGAGAGAGGCACTTTGCACCACAGACAGATAGCAAGAAGGGAAAGACAGAGAGTGAGAAAAAAGAGGAGTCAGTCGCTCCTGGGGAAGGGAGAG AGTGAGACTGGGAGAAAGAGAAGCACAGAAAGTGTGTGTAAAACGGAGTAAAGAAAGAAAAAAAAAAAACTACCCTTAAAGCACATTTAAAAAAAAAAAA AACTCTGGCAATTCAAGAAAGAAACAGGCTACGTTTAAAGAGCATAGAGACAATGAAAGGCTAAAGAAAATTTTAAAATCTCTGCCACAGTCTCATAGGT GCTTGGAAATGAAAGTAGAACTGCCTGTCTTTAACGGACTCTGACAGAGGTAACTGGATTAGGGACGAGTACGCCAGCTTTTTTTTTTTTTTTTTTTTTT TTTTTTTAACATCTTAAATCCTGAAAAAAAAAAAAAAAAAAAAAAAAAGGCAGCAGCTCCGAATTGAATGAATTGATGGGCACACTCCAACTGCTGGGCT GGAGAGACTGGACTTAGTCTTGCCATTTCTGCTTCTTTGAAAGAGGAGACAACTTGGGCTTCCTTTTAATTTAGTTTTTTTTCCCCTTCTCCCCCAACCC CCAACCTTCCCCCTTACCTCCCCCACCCCCTTTATCACCACCCCCCTTTTAAATAAGAGGGTGAAGGGGAACCAGAGCGCACAAGGGAACTGACTCAGGA GGCAGAGAAGATGGGCATCCTCAGCGTAGACTTGCTGATCACACTGCAAATTCTGCCAGTTTTTTTCTCCAACTGCCTCTTCCTGGCTCTCTATGACTCG GTCATTCTGCTCAAGCACGTGGTGCTGCTGTTGAGCCGCTCCAAGTCCACTCGCGGAGAGTGGCGGCGCATGCTGACCTCAGAGGGACTGCGCTGCGTCT GGAAGAGCTTCCTCCTCGATGCCTACAAACAGGTGAAATTGGGTGAGGATGCCCCCAATTCCAGTGTGGTGCATGTCTCCAGTACAGAAGGAGGTGACAA CAGTGGCAATGGTACCCAGGAGAAGATAGCTGAGGGAGCCACATGCCACCTTCTTGACTTTGCCAGCCCTGAGCGCCCACTAGTGGTCAACTTTGGCTCA GCCACTTGACCTCCTTTCACGAGCCAGCTGCCAGCCTTCCGCAAACTGGTGGAAGAGTTCTCCTCAGTGGCTGACTTCCTGCTGGTCTACATTGATGAGG CTCATCCATCAGATGGCTGGGCGATACCGGGGGACTCCTCTTTGTCTTTTGAGGTGAAGAAGCACCAGAACCAGGAAGATCGATGTGCAGCAGCCCAGCA GCTTCTGGAGCGTTTCTCCTTGCCGCCCCAGTGCCGAGTTGTGGCTGACCGCATGGACAATAACGCCAACATAGCTTACGGGGTAGCCTTTGAACGTGTG TGCATTGTGCAGAGACAGAAAATTGCTTATCTGGGAGGAAAGGGCCCCTTCTCCTACAACCTTCAAGAAGTCCGGCATTGGCTGGAGAAGAATTTCAGCA AGAGATGAAAGAAAACTAGATTAGCTGGTTAAAGGTATGATTATAAGAGAGCTTATTGTTTTAAAAAGTTATATAAAGGCAAGGAAATTAAGAACTGAAT CCATATTTCAACAGAGCCCTATTGGCTTACTGAAAGACAGGAGTTTATCTATCGGAAGAACATGAATCTCTAACAGCTCCATACTTCTTTCACTACTCAA ATGGCATTGGGCTGAGTAAGTAACCATATCACCTCTCTTCTTAGTAAAAAGCCCTATGTGAAAAGATCCCAAGATGGAGAGGAAGAAACGCTAATTCAGC ATGTGTTCATTCTGCATTGAGAAGGAACTGATACATCTGATGCATGCTTTGAGACCAGAAGAAAAGACTTACCTGAATAATTACTACATTAGGGAAGCTA CTGTCTACGTTAAGATAAAGGGTATTGCCTTGGCTCTATTTGGCATGGATGGAGCCCAGTTGGAAAATTCCCAAATATTACAACAAGTCCTTGAACCCAG GCCATGTGGTTAGACGTTGGTGTTAAGGTTAGACCTTATGTTAGAGTCATTTCTGATGTTCCAGCTTCTAGCCATGTAGTGCTCTCAGTCTTCATACCCC AGAAATTATTGGTATATTTGTAGATACCGAGAATGATCCCTCAGTCTGAGAGGTTAGAATGATCATCTGTAATCTGAGGGTTAATTTCTAGGCAGGTGGA GAGAGTGGTAAAAAAGAAATGAAATTGACAAGCTAGGAAAGAGGAGGCAGAAAGATTTGGAAAATTCACAGAGTTTCACCCTTAAGCTGTAGAGAGTGGG TCACATTTGTTAGCCACGGAAACATAGAAACATACACAAGGCCAGAAAAAGAAGAAGGAGCTCAACTAAAAGTGGCATAGAGAATACACATATAAAAACA ATATATTTGTCATATGCTCCTAGAGAGGAGAAAGGGGTGATTGAAAGAAAAAAAAATACTTAAATATTTGTAATTGTGAGGGGTTTCTTTTGGAAATAAT TACTTTTGAACCATGTATGTGGTATGTATATTTTCAGTGGGTTAATTATACCCCATGATACCTATTAAAGGAAAACCAGTGGGTCTGGTGGTGCTGGTCT TTTCCTCCCCATTCCTACAATTTCTATGTGGCCCAAGTCATTCCTAATCTTGGTCTCTATAGCAGTGTTCTCTCTGAATGCTGAGCTGAAGAAATTATAC GTACATACACACATACATACATACATACAAATATATGTATATATATTCTCAGCTGCTGCGGGAGGTAGGTACCATGGCCATTCAGCACAGCCTTGATTTC CTCCCAAAGTAGGTGAGCTATAGTGAAGAATAGGTGCAAACAAACAAGCTTACTTCCATTGCAAAATAGAAGAAGAGGAAGTTAGAGATAATTCTGATCA ATCATTTTGGAGGCTTTGTTATAAGGCAACCCCCGGTATATCATGGAATTTCCATTGACATTTGAATTTGGACTTGGATCTTCCCTTGGTCCCATTAGCT GAGGTTTAGTAATCTAAAGTCCCTATAGTATATGATTATAATGCTATTTTAAAAAATATATATATAAAATATTTTTTTCTTTTTAAAATAGACACTATAG TTTTACCCATAAGTAATATTTAAAGATTATAGCTCCCAAAAGAATGGACCAACCACTTTCGTATCATAATTTCTTTTTGGTAAATATGAGACTATTATGA AATCATAGTATATGATTGTATTTAAAGGTACAATCAAAGGATCTTTTGTCCATTCCATTAATAACTGAATAAAAAATAAATAAAATGGATAGAAAAAAAC TAAAGTTGAAAATACATTCTTAAACTAGTTGTCTGAATTGAGAAAAGAGTGAGAACTAGGTGTGCAAGAACCAAACGTATTTTATTTTATTTTTTAAATG GGAGCAACATATCAGTCGTGTCACCAGCTGGTATATTGTGTAAATATTAAAGCTCCATTGGGACTGATTTTTCATGGCAACATCAGCTTTCTAATGTTCT AAATTCTATAAAAACCACCCACAAAGAAACAAAGCAAATTTCATTATCTAATGAGTTGCTGGAAAATCATATTGAGAATAATTATTTCAGATTCCTCAGT TGTTAACTTCTACATTCAAGGCTTATCTCTGCCCCCATTGATTTTTAACCTCAAAATGGTGTTGAGATTTACGTGGAACCCTAAAGCAGTAAAATAAAAA ACCTGGTTGCAGCACATTCACACTGTTGTCCTTAAAATTCCCCTTTTTTCTCTATGTACGATAAAGTAACAGTATGTCAGATAAGCCGGTGGGGGGATGA GATTAGGCTGAGGCAGTGCTAGTCAACTGGGGAAAAGGATGATGGAAAAATCACCCAGTTGTGCTATATTTTTAAAGAAGGAGGTCGTTTATGTGTGCAG ACAATTCTCCCTGAGGTTAGCCCAATGGAGAAATGAAGCAGAGGAAGGAAACATAGAAAGACATGGGCTATCAGGGAGGAAGATGTTCAATAGAACATGC AAGAATTTCTGGAAGAAAGGCTGTGGAAGGGCCAATGGAGAAAATGAATGGACAAAGCTCAGGAATCCTACGCTATGTAGAATGTCTTGGTGTTATCAGG GTTAAGCCTGTAATTATGTAACCTATTTATCGCAACATGAATTTTTATGATTTCTTGTGATGTATTCTTTTATGAAATTAACAAGAACTCATTATTTTGA GGTAGAGGAAAATCAATGCTTTATCTGATATGCTGAGAAATTATTAGATTGCCAATACTCATGTGCGTTTCATGTGTTTTATAAGGTTTGTTCCTTTGAA GAATTGTAGTTCTTAGTCCCACAGGGAAATGTGTATCTATTTATATATCATAGTATAAATCTATGATATATTTATATCATATATAAAAGTCTGAGTTCTC TTTCTTAGTCCCTAATCATGTTTCTCCCATAGGCTGTGTTTACATGGAGCTATCGGTTTAGCCTTTTAAGCTTCATTAGCTTGTCTATTATTGAAATAGT TTCCAAGAAATTTTAGATATTATCATAACATCTGGGTCTACTCAAACACTTATTGTTTGAAAGACTTATGTCTTGGACCTATCAAAAACTGACTTTATTT ATTGCTTAGTGAAAATACTAGTGGGATCAACAATGATTTTCTTGAATGGGCATGAATGGAGATGCCCGCACAGTAATGTAGAAATGTTTCATACAGCTAT TAAAATGTAACTGACCTCCTTAGAGGCAGATTAGTAACTGTTCCTACTTTGTATAGCTAAGTGACAGTCACTTAACTTACATGACTTTCTTTTTTCACAT TGGGTCTCTGGTCCTGTGTCTTCACCTCATTTATAGCACGTCTCCTTGATTTTTGGTAGTATCAACTTCCCAGTGATCTGTTCAGTTAAGTTCTTCTCCC GTTAACCAGGAAGTGCTTATTCTCTCATCACAGTGGGAAGAATAGCCTATTGTCTTTCATTTTGCCTGAGTGTATTTTACTATTTGGGCTCTGAAATAAA AATTATGAAATATGGTGAGGTCACATGTTGGTGCTGCCTTGCTGCATAAAATTCTAGAGGGCAGGTTAGAGACAGTATGTATGCCTTCGGGAAAATTCAA AGGTGGATTACAAGGTGTCCTCAGCATGCCCTATGGCCTATGTGCGAAGCAAGAAGAATTGACTGATTTACAGGACTTCTCTTTATGTCAATCTTAAGAG GATGGATGAATCTGGACATTTGTTCCACCCGACCTCTGACTGATGGTTTGGAAAATAACTTTAATTAGGATCATATGACCATTGAAAAAGGAAAAATGTA GACTCTGACTTCCGTCCCACTGAAGGATTAATGAAAACCTTTACTAGCATTTAGAGCTTTTCAGAACATCCCCACTGTCATGTGTCTCAGCAGTGGAGAC TGCAAGTAAGGCTTTTAATTTTAGGAGGTTTTTTTTTTTTTTTTTTTTTCCCTAAATGGTATGGCCAAAAGTCAGAGTTAAAATATATATAGTTAGATTC CAACTTCCTCCTTCACTCTAAAAATAGAATCCAAACCCACTCTTCATATATGCTTCCAGAATGGGGCTTAAGTACCAATCTCTGCTTTGCAATGGGCACA ATCTTGGTCATGTCCTGAGGCTCTCTAAGAAAAGAGAGGATCTAGGATGGGAGAGCTAGAAAGTTGCTAACTGGGAAGAACAAGGCCCTGAGGGCTTGGT CTACCAATCTGGGAAGATTTGAAAACAAACTTCTCGCAACTGAAGGAAGGCTGAAGGCTGCTGCAAGTCATTGAGTGACTTTAGGATGAGCAAAACATTG GGCCACTTCCTAATGCCCTATGTGTATAGTACCAGAAGCAAGGTCTCAGACTTAACAGACCCAGCTCTGTTCCAAGGTGAGTCTGAACCAATAGAAAGCA AACATGTGCAGATATCCAAACAAGACTGCTCATGCAAGTCGGGGCTGGCTACCCGTCTTAGGCAGCAACAGCAGAGCTCCAGGGAGCTTATTCAATATTT ACTGAGACTTCGAAGACCCAGCAGATGTTTAATGAAGTCACTATTTTGGCTCAAACCCTCCACTTCTCCCCCTCCCCTCAAAAAGCCAACAGGTAAACAC ATAAATGAAAGAAACCCACAGAAGGGGATGGGAAATAAAGAAAATTCTCTCAAGACTTCTCCAGGCCCATGTCACTGGTCAGCGTGGTTTTTATGTGTAT TAGGATTGGGGGATGTGAAGAAATAAGTATCCAGTACTTTATAACCAAAGCAATTAAATGATATTGGGTAGGGAATGTTGGCCAGTTTTGTTTAGTTTTG CATCACATTGTCACCAGACTCACTAGCCCAAGTAATCGGGCGCCCGAAGAGGGAGACAGAGATGTGCAGAGTTGACCAGTGTGCGGATGATAACTACTGA CGAAAGAGTCATCGACTCAGTTAGTGGTTGGATGTAGTCACATTAGTTTGCCTCTCCCCATCTTTGTCTCCCTGGCAAGGAGAATATGCGGGACATGATG CTAAGAGTCCTGGGTAAATGTGGTGAGAATGCACGCGTGCATATGCTACACATATGTGCTTCTCAGTTGCAGAAAATGAACTGCTTTGGGAGATTATCAG TAGAAAGAGTGTTATCATATTGGTGCTGAGTGCTATGTGTGCTTATACAATTTGTTCTTGTATTTTAATAAACTTTGAATAAAAGAATAAAGCTA

refseq_DIO2.F1 (forward primer)
refseq_DIO2.R1 (reverse primer)
Single exon skipping

Frame 0

GCRERHFAPQTDSKKGKTESEKKEESVAPGEGR E*DWEKEKHRKCV*NGVKKEKKKTTLKAHLKKKK TLAIQERNRLRLKSIETMKG*RKF*NLCHSLIG AWK*K*NCLSLTDSDRGNWIRDEYASFFFFFFF FFLTS*ILKKKKKKKKRQQLRIE*IDGHTPTAGL ERLDLVLPFLLL*KRRQLGLPFNLVFFPLLPQP PTFPLTSPTPFITTPLLNKRVKGNQSAQGN*LR RQRRWASSA*TC*SHCKFCQFFSPTASSWLSMTR SFCSSTWCCC*AAPSPLAESGGAC*PQRDCAAS GRASSSMPTNR*NWVRMPPIPVWCMSPVQKEVT TVAMVPRRR*LREPHATFLTLPALSAH*WSTLAQ PLDLLSRASCQPSANWWKSSPQWLTSCWSTLMR LIHQMAGRYRGTPLCLLR*RSTRTRKIDVQQPS SFWSVSPCRPSAELWLTAWTITPT*LTG*PLNVC ALCRDRKLLIWEERAPSPTTFKKSGIGWRRISA RDERKLD*LVKGMIIRELIVLKSYIKARKLRTE SIFQQSPIGLLKDRSLSIGRT*ISNSSILLSLLK WHWAE*VTISPLFLVKSPM*KDPKMERKKR*FS MCSFCIEKELIHLMHALRPEEKTYLNNYYIREA TVYVKIKGIALALFGMDGAQLENSQILQQVLEPR PCG*TLVLRLDLMLESFLMFQLLAM*CSQSSYP RNYWYICRYRE*SLSLRG*NDHL*SEG*FLGRW REW*KRNEIDKLGKRRQKDLENSQSFTLKL*RVG HIC*PRKHRNIHKARKRRRSSTKSGIENTHIKT IYLSYAPREEKGVIERKKNT*IFVIVRGFFWK* LLLNHVCGMYIFSGLIIPHDTY*RKTSGSGGAGL FLPIPTISMWPKSFLILVSIAVFSLNAELKKLY VHTHIHTYIQIYVYIFSAAAGGRYHGHSAQP*F PPKVGEL**RIGANKQAYFHCKIEEEEVRDNSDQ SFWRLCYKATPGISWNFH*HLNLDLDLPLVPLA EV**SKVPIVYDYNAILKNIYIKYFFLFKIDTI VLPISNI*RL*LPKEWTNHFRIIISFW*I*DYYE IIVYDCI*RYNQRIFCPFH**LNKK*IKWIEKN *S*KYILKLVV*IEKRVRTRCARTKRILFYFLN GSNISVVSPAGILCKY*SSIGTDFSWQHQLSNVL NSIKTTHKETKQISLSNELLENHIENNYFRFLS C*LLHSRLISAPIDF*PQNGVEIYVEP*SSKIK NLVAAHSHCCP*NSPFFSMYDKVTVCQISRWGDE IRLRQC*STGEKDDGKITQLCYIFKEGGRLCVQ TILPEVSPMEK*SRGRKHRKTWAIREEDVQ*NM QEFLEERLWKGQWRK*MDKAQESYAM*NVLVLSG LSL*LCNLFIAT*IFMISCDVFFYEINKNSLF* GRGKSMLYLIC*EIIRLPILMCVSCVL*GLFL* RIVVLSPTGKCVSIYIS*YKSMIYLYHI*KSEFS FLVPNHVSPIGCVYMELSV*PFKLH*LVYY*NS FQEILDIIITSGSTQTLIV*KTYVLDLSKTDFI YCLVKILVGSTMIFLNGHEWRCPHSNVEMFHTAI KM*LTSLEAD**LFLLCIAK*QSLNLHDFLFSH WVSGPVSSPHL*HVSLIFGSINFPVICSVKFFS R*PGSAYSLITVGRIAYCLSFCLSVFYYLGSEIK IMKYGEVTCWCCLAA*NSRGQVRDSMYAFGKIQ RWITRCPQHALWPMCEARRID*FTGLLFMSILR GWMNLDICSTRPLTDGLENNFN*DHMTIEKGKM* TLTSVPLKD**KPLLAFRAFQNIPTVMCLSSGD CK*GF*F*EVFFFFFFSLNGMAKSQS*NIYS*I PTSSFTLKIESKPTLHICFQNGA*VPISALQWAQ SWSCPEAL*EKRGSRMGELESC*LGRTRP*GLG LPIWEDLKTNFSQLKEG*RLLQVIE*L*DEQNI GPLPNALCV*YQKQGLRLNRPSSVPR*V*TNRKQ TCADIQTRLLMQVGAGYPS*AATAELQGAYSIF TETSKTQQMFNEVTILAQTLHFSPSPQKANR*T HK*KKPTEGDGK*RKFSQDFSRPMSLVSVVFMCI RIGGCEEISIQYFITKAIK*YWVGNVGQFCLVL HHIVTRLTSPSNRAPEEGDRDVQS*PVCG**LL TKESSTQLVVGCSHISLPLPIFVSLARRICGT*C *ESWVNVVRMHACICYTYVLLSCRK*TALGDYQ *KECYHIGAECYVCLYNLFLYFNKL*IKE*S

Frame 1

AAERGTLHHRQIARRERQRVRKKRSQSLLGKGE SETGRKRSTESVCKTE*RKKKKKLPLKHI*KKK KLWQFKKETGYV*RA*RQ*KAKENFKISATVS*V LGNESRTACL*RTLTEVTGLGTSTPAFFFFFFF FF*HLKS*KKKKKKKKGSSSELNELMGTLQLLG WRDWT*SCHFCFFERGDNLGFLLI*FFFPFSPNP QPSPLPPPPPLSPPPF*IRG*RGTRAHKGTDSG GREDGHPQRRLADHTANSASFFLQLPLPGSL*L GHSAQARGAAVEPLQVHSRRVAAHADLRGTALRL EELPPRCLQTGEIG*GCPQFQCGACLQYRRR*Q QWQWYPGEDS*GSHMPPS*LCQP*APTSGQLWL SHLTSFHEPAASLPQTGGRVLLSG*LPAGLH**G SSIRWLGDTGGLLFVF*GEEAPEPGRSMCSSPA ASGAFLLAAPVPSCG*PHGQ*RQHSLRGSL*TC VHCAETENCLSGRKGPLLLQPSRSPALAGEEFQQ EMKEN*ISWLKV*L*ESLLF*KVI*RQGN*ELN PYFNRALLAY*KTGVYLSEEHESLTAPYFFHYS NGIGLSK*PYHLSS**KALCEKIPRWRGRNANSA CVHSALRRN*YI*CML*DQKKRLT*IITTLGKL LSTLR*RVLPWLYLAWMEPSWKIPKYYNKSLNP GHVVRRWC*G*TLC*SHF*CSSF*PCSALSLHTP EIIGIFVDTENDPSV*EVRMIICNLRVNF*AGG ESGKKEMKLTS*ERGGRKIWKIHRVSPLSCREW VTFVSHGNIETYTRPEKEEGAQLKVA*RIHI*KQ YICHMLLERRKG*LKEKKILKYL*L*GVSFGNN YF*TMYVVCIFSVG*LYPMIPIKGKPVGLVVLV FSSPFLQFLCGPSHS*SWSL*QCSL*MLS*RNYT YIHTYIHTYKYMYIYSQLLREVGTMAIQHSLDF LPK*VSYSEE*VQTNKLTSIAK*KKRKLEIILI NHFGGFVIRQPPVYHGISIDI*IWTWIFPWSH*L RFSNLKSL*YMIIMLF*KIYI*NIFFFLK*TL* FYP*VIFKDYSSQKNGPTTFVS*FLFGKYETIM KS*YMIVFKGTIKGSFVHSINN*IKNK*NG*KKT KVENTFLN*LSELRKE*ELGVQEPNVFYFIF*M GATYQSCHQLVYCVNIKAPLGLIFHGNISFLMF *IL*KPPTKKQSKFHYLMSCWKIILRIIISDSSV VNFYIQGLSLPPLIFNLKMVLRFTWNPKAVK*K TWLQHIHTVVLKIPLFSLCTIK*QYVR*AGGGM RLG*GSASQLGKRMMEKSPSCAIFLKKEVVYVCR QFSLRLAQWRNEAEEGNIERHGLSGRKMFNRTC KNFWKKGCGRANGENEWTKLRNPTLCRMSWCYQ G*ACNYVTYLSQHEFL*FLVMYSFMKLTRTHYFE VEENQCFI*YAEKLLDCQYSCAFHVFYKVCSFE EL*FLVPQGNVYLFIYHSINL*YIYIIYKSLSS LS*SLIMFLP*AVFTWSYRFSLLSFISLSIIEIV SKKF*ILS*HLGLLKHLLFERLMSWTYQKLTLF IA**KY*WDQQ*FS*MGMNGDARTVM*KCFIQL LKCN*PP*RQISNCSYFV*LSDSHLTYMTFFFHI GSLVLCLHLIYSTSP*FLVVSTSQ*SVQLSSSP VNQEVLILSSQWEE*PIVFHFA*VYFTIWALK* KL*NMVRSHVGAALLHKILEGRLETVCMPSGKFK GGLQGVLSMPYGLCAKQEELTDLQDFSLCQS*E DG*IWTFVPPDL*LMVWKITLIRII*PLKKEKC RL*LPSH*RINENLY*HLELFRTSPLSCVSAVET ASKAFNFRRFFFFFFFP*MVWPKVRVKIYIVRF QLPPSL*K*NPNPLFIYASRMGLKYQSLLCNGH NLGHVLRLSKKREDLGWES*KVANWEEQGPEGLV YQSGKI*KQTSRN*RKAEGCCKSLSDFRMSKTL GHFLMPYVYSTRSKVSDLTDPALFQGESEPIES KHVQISKQDCSCKSGLATRLRQQQQSSRELIQYL LRLRRPSRCLMKSLFWLKPSTSPPPLKKPTGKH INERNPQKGMGNKENSLKTSPGPCHWSAWFLCV LGLGDVKK*VSSTL*PKQLNDIG*GMLASFV*FC ITLSPDSLAQVIGRPKRETEMCRVDQCADDNY* RKSHRLS*WLDVVTLVCLSPSLSPWQGEYAGHD AKSPG*MW*ECTRAYATHMCFSVAENELLWEIIS RKSVIILVLSAMCAYTICSCILINFE*KNKA

Frame 2

LQREALCTTDR*QEGKDRE*EKRGVSRSWGRER VRLGEREAQKVCVKRSKERKKKNYP*STFKKKK NSGNSRKKQATFKEHRDNERLKKILKSLPQSHR CLEMKVELPVFNGL*QR*LD*GRVRQLFFFFFFF FFNILNPEKKKKKKKKAAAPN*MN*WAHSNCWA GETGLSLAISASLKEETTWASF*FSFFSPSPPT PNLPPYLPHPLYHHPPFK*EGEGEPERTRELTQE AEKMGILSVDLLITLQILPVFFSNCLFLALYDS VILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCV WKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDN SGNGTQEKIAEGATCHLLDFASPERPLVVNFGS AT*PPFTSQLPAFRKLVEEFSSVADFLLVYIDE AHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQ LLERFSLPPQCRVVADRMDNNANIAYGVAFERV CIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFS KR*KKTRLAG*RYDYKRAYCFKKLYKGKEIKN*I HISTEPYWLTERQEFIYRKNMNL*QLHTSFTTQ MALG*VSNHITSLLSKKPYVKRSQDGEEETLIQ HVFILH*EGTDTSDACFETRRKDLPE*LLH*GSY CLR*DKGYCLGSIWHGWSPVGKFPNITTSP*TQ AMWLDVGVKVRPYVRVISDVPASSHVVLSVFIP QKLLVYL*IPRMIPQSERLE*SSVI*GLISRQVE RVVKKK*N*QARKEEAERFGKFTEFHP*AVESG SHLLATET*KHTQGQKKKKELN*KWHREYTYKN NIFVICS*RGERGD*KKKKYLNICNCEGFLLEII TFEPCMWYVYFQWVNYTP*YLLKENQWVWWCWS FPPHSYNFYVAQVIPNLGLYSSVLSEC*AEEII RTYTHTYIHTNICIYILSCCGR*VPWPFSTALIS SQSR*AIVKNRCKQTSLLPLQNRRRGS*R*F*S IILEALL*GNPRYIMEFPLTFEFGLGSSLGPIS *GLVI*SPYSI*L*CYFKKYIYKIFFSF*NRHYS FTHK*YLKIIAPKRMDQPLSYHNFFLVNMRLL* NHSI*LYLKVQSKDLLSIPLITE*KINKMDRKK LKLKIHS*TSCLN*EKSEN*VCKNQTYFILFFKW EQHISRVTSWYIV*ILKLHWD*FFMATSAF*CS KFYKNHPQRNKANFII**VAGKSY*E*LFQIPQ LLTSTFKAYLCPH*FLTSKWC*DLRGTLKQ*NKK PGCSTFTLLSLKFPFFLYVR*SNSMSDKPVGG* D*AEAVLVNWGKG*WKNHPVVLYF*RRRSFMCA DNSP*G*PNGEMKQRKET*KDMGYQGGRCSIEHA RISGRKAVEGPMEKMNGQSSGILRYVECLGVIR VKPVIM*PIYRNMNFYDFL*CILL*N*QELIIL R*RKINALSDMLRNY*IANTHVRFMCFIRFVPLK NCSS*SHREMCIYLYIIV*IYDIFISYIKV*VL FLSP*SCFSHRLCLHGAIGLAF*ASLACLLLK* FPRNFRYYHNIWVYSNTYCLKDLCLGPIKN*LYL LLSENTSGINNDFLEWA*MEMPAQ*CRNVSYSY *NVTDLLRGRLVTVPTLYS*VTVT*LT*LSFFT LGLWSCVFTSFIARLLDFW*YQLPSDLFS*VLLP LTRKCLFSHHSGKNSLLSFILPECILLFGL*NK NYEIW*GHMLVLPCCIKF*RAG*RQYVCLRENS KVDYKVSSACPMAYVRSKKN*LIYRTSLYVNLKR MDESGHLFHPTSD*WFGK*L*LGSYDH*KRKNV DSDFRPTEGLMKTFTSI*SFSEHPHCHVSQQWR LQVRLLILGGFFFFFFFPKWYGQKSELKYI*LDS NFLLHSKNRIQTHSSYMLPEWGLSTNLCFAMGT ILVMS*GSLRKERI*DGRARKLLTGKNKALRAW STNLGRFENKLLATEGRLKAAASH*VTLG*AKHW ATS*CPMCIVPEARSQT*QTQLCSKVSLNQ*KA NMCRYPNKTAHASRGWLPVLGSNSRAPGSLFNI Y*DFEDPADV**SHYFGSNPPLLPLPSKSQQVNT *MKETHRRGWEIKKILSRLLQAHVTGQRGFYVY *DWGM*RNKYPVLYNQSN*MILGRECWPVLFSF ASHCHQTH*PK*SGARRGRQRCAELTSVRMITTD ERVIDSVSGWM*SH*FASPHLCLPGKENMRDMM LRVLGKCGENARVHMLHICASQLQKMNCFGRLS VERVLSYWC*VLCVLIQFVLVF**TLNKRIKL

Sequence NM_001007023

GGCTGCAGAGAGAGGCACTTTGCACCACAGACAGATAGCAAGAAGGGAAAGACAGAGAGTGAGAAAAAAGAGGAGTCAGTCGCTCCTGGGGAAGGGAGAG AGTGAGACTGGGAGAAAGAGAAGCACAGAAAGTGTGTGTAAAACGGAGTAAAGAAAGAAAAAAAAAAAACTACCCTTAAAGCACATTTAAAAAAAAAAAA AACTCTGGCAATTCAAGAAAGAAACAGGCTACGTTTAAAGAGCATAGAGACAATGAAAGGCTAAAGAAAATTTTAAAATCTCTGCCACAGTCTCATAGGT GCTTGGAAATGAAAGTAGAACTGCCTGTCTTTAACGGACTCTGACAGAGGTAACTGGATTAGGGACGAGTACGCCAGCTTTTTTTTTTTTTTTTTTTTTT TTTTTTTAACATCTTAAATCCTGAAAAAAAAAAAAAAAAAAAAAAAAAGGCAGCAGCTCCGAATTGAATGAATTGATGGGCACACTCCAACTGCTGGGCT GGAGAGACTGGACTTAGTCTTGCCATTTCTGCTTCTTTGAAAGAGGAGACAACTTGGGCTTCCTTTTAATTTAGTTTTTTTTCCCCTTCTCCCCCAACCC CCAACCTTCCCCCTTACCTCCCCCACCCCCTTTATCACCACCCCCCTTTTAAATAAGAGGGTGAAGGGGAACCAGAGCGCACAAGGGAACTGACTCAGGA GGCAGAGAAGATGGGCATCCTCAGCGTAGACTTGCTGATCACACTGCAAATTCTGCCAGTTTTTTTCTCCAACTGCCTCTTCCTGGCTCTCTATGACTCG GTCATTCTGCTCAAGCACGTGGTGCTGCTGTTGAGCCGCTCCAAGTCCACTCGCGGAGAGTGGCGGCGCATGCTGACCTCAGAGGGACTGCGCTGCGTCT GGAAGAGCTTCCTCCTCGATGCCTACAAACAGCTAAATTGTCCTCCATCAGGTTTTAGCAAAGATGGACACATTTTATGACTAGTATATGAAGCTTATAA AAGCAGACTACTGGTCTACTCACATTTGGATTTATGGATGGTGAAATTGGGTGAGGATGCCCCCAATTCCAGTGTGGTGCATGTCTCCAGTACAGAAGGA GGTGACAACAGTGGCAATGGTACCCAGGAGAAGATAGCTGAGGGAGCCACATGCCACCTTCTTGACTTTGCCAGCCCTGAGCGCCCACTAGTGGTCAACT TTGGCTCAGCCACTTGACCTCCTTTCACGAGCCAGCTGCCAGCCTTCCGCAAACTGGTGGAAGAGTTCTCCTCAGTGGCTGACTTCCTGCTGGTCTACAT TGATGAGGCTCATCCATCAGATGGCTGGGCGATACCGGGGGACTCCTCTTTGTCTTTTGAGGTGAAGAAGCACCAGAACCAGGAAGATCGATGTGCAGCA GCCCAGCAGCTTCTGGAGCGTTTCTCCTTGCCGCCCCAGTGCCGAGTTGTGGCTGACCGCATGGACAATAACGCCAACATAGCTTACGGGGTAGCCTTTG AACGTGTGTGCATTGTGCAGAGACAGAAAATTGCTTATCTGGGAGGAAAGGGCCCCTTCTCCTACAACCTTCAAGAAGTCCGGCATTGGCTGGAGAAGAA TTTCAGCAAGAGATGAAAGAAAACTAGATTAGCTGGTTAAAGGTATGATTATAAGAGAGCTTATTGTTTTAAAAAGTTATATAAAGGCAAGGAAATTAAG AACTGAATCCATATTTCAACAGAGCCCTATTGGCTTACTGAAAGACAGGAGTTTATCTATCGGAAGAACATGAATCTCTAACAGCTCCATACTTCTTTCA CTACTCAAATGGCATTGGGCTGAGTAAGTAACCATATCACCTCTCTTCTTAGTAAAAAGCCCTATGTGAAAAGATCCCAAGATGGAGAGGAAGAAACGCT AATTCAGCATGTGTTCATTCTGCATTGAGAAGGAACTGATACATCTGATGCATGCTTTGAGACCAGAAGAAAAGACTTACCTGAATAATTACTACATTAG GGAAGCTACTGTCTACGTTAAGATAAAGGGTATTGCCTTGGCTCTATTTGGCATGGATGGAGCCCAGTTGGAAAATTCCCAAATATTACAACAAGTCCTT GAACCCAGGCCATGTGGTTAGACGTTGGTGTTAAGGTTAGACCTTATGTTAGAGTCATTTCTGATGTTCCAGCTTCTAGCCATGTAGTGCTCTCAGTCTT CATACCCCAGAAATTATTGGTATATTTGTAGATACCGAGAATGATCCCTCAGTCTGAGAGGTTAGAATGATCATCTGTAATCTGAGGGTTAATTTCTAGG CAGGTGGAGAGAGTGGTAAAAAAGAAATGAAATTGACAAGCTAGGAAAGAGGAGGCAGAAAGATTTGGAAAATTCACAGAGTTTCACCCTTAAGCTGTAG AGAGTGGGTCACATTTGTTAGCCACGGAAACATAGAAACATACACAAGGCCAGAAAAAGAAGAAGGAGCTCAACTAAAAGTGGCATAGAGAATACACATA TAAAAACAATATATTTGTCATATGCTCCTAGAGAGGAGAAAGGGGTGATTGAAAGAAAAAAAAATACTTAAATATTTGTAATTGTGAGGGGTTTCTTTTG GAAATAATTACTTTTGAACCATGTATGTGGTATGTATATTTTCAGTGGGTTAATTATACCCCATGATACCTATTAAAGGAAAACCAGTGGGTCTGGTGGT GCTGGTCTTTTCCTCCCCATTCCTACAATTTCTATGTGGCCCAAGTCATTCCTAATCTTGGTCTCTATAGCAGTGTTCTCTCTGAATGCTGAGCTGAAGA AATTATACGTACATACACACATACATACATACATACAAATATATGTATATATATTCTCAGCTGCTGCGGGAGGTAGGTACCATGGCCATTCAGCACAGCC TTGATTTCCTCCCAAAGTAGGTGAGCTATAGTGAAGAATAGGTGCAAACAAACAAGCTTACTTCCATTGCAAAATAGAAGAAGAGGAAGTTAGAGATAAT TCTGATCAATCATTTTGGAGGCTTTGTTATAAGGCAACCCCCGGTATATCATGGAATTTCCATTGACATTTGAATTTGGACTTGGATCTTCCCTTGGTCC CATTAGCTGAGGTTTAGTAATCTAAAGTCCCTATAGTATATGATTATAATGCTATTTTAAAAAATATATATATAAAATATTTTTTTCTTTTTAAAATAGA CACTATAGTTTTACCCATAAGTAATATTTAAAGATTATAGCTCCCAAAAGAATGGACCAACCACTTTCGTATCATAATTTCTTTTTGGTAAATATGAGAC TATTATGAAATCATAGTATATGATTGTATTTAAAGGTACAATCAAAGGATCTTTTGTCCATTCCATTAATAACTGAATAAAAAATAAATAAAATGGATAG AAAAAAACTAAAGTTGAAAATACATTCTTAAACTAGTTGTCTGAATTGAGAAAAGAGTGAGAACTAGGTGTGCAAGAACCAAACGTATTTTATTTTATTT TTTAAATGGGAGCAACATATCAGTCGTGTCACCAGCTGGTATATTGTGTAAATATTAAAGCTCCATTGGGACTGATTTTTCATGGCAACATCAGCTTTCT AATGTTCTAAATTCTATAAAAACCACCCACAAAGAAACAAAGCAAATTTCATTATCTAATGAGTTGCTGGAAAATCATATTGAGAATAATTATTTCAGAT TCCTCAGTTGTTAACTTCTACATTCAAGGCTTATCTCTGCCCCCATTGATTTTTAACCTCAAAATGGTGTTGAGATTTACGTGGAACCCTAAAGCAGTAA AATAAAAAACCTGGTTGCAGCACATTCACACTGTTGTCCTTAAAATTCCCCTTTTTTCTCTATGTACGATAAAGTAACAGTATGTCAGATAAGCCGGTGG GGGGATGAGATTAGGCTGAGGCAGTGCTAGTCAACTGGGGAAAAGGATGATGGAAAAATCACCCAGTTGTGCTATATTTTTAAAGAAGGAGGTCGTTTAT GTGTGCAGACAATTCTCCCTGAGGTTAGCCCAATGGAGAAATGAAGCAGAGGAAGGAAACATAGAAAGACATGGGCTATCAGGGAGGAAGATGTTCAATA GAACATGCAAGAATTTCTGGAAGAAAGGCTGTGGAAGGGCCAATGGAGAAAATGAATGGACAAAGCTCAGGAATCCTACGCTATGTAGAATGTCTTGGTG TTATCAGGGTTAAGCCTGTAATTATGTAACCTATTTATCGCAACATGAATTTTTATGATTTCTTGTGATGTATTCTTTTATGAAATTAACAAGAACTCAT TATTTTGAGGTAGAGGAAAATCAATGCTTTATCTGATATGCTGAGAAATTATTAGATTGCCAATACTCATGTGCGTTTCATGTGTTTTATAAGGTTTGTT CCTTTGAAGAATTGTAGTTCTTAGTCCCACAGGGAAATGTGTATCTATTTATATATCATAGTATAAATCTATGATATATTTATATCATATATAAAAGTCT GAGTTCTCTTTCTTAGTCCCTAATCATGTTTCTCCCATAGGCTGTGTTTACATGGAGCTATCGGTTTAGCCTTTTAAGCTTCATTAGCTTGTCTATTATT GAAATAGTTTCCAAGAAATTTTAGATATTATCATAACATCTGGGTCTACTCAAACACTTATTGTTTGAAAGACTTATGTCTTGGACCTATCAAAAACTGA CTTTATTTATTGCTTAGTGAAAATACTAGTGGGATCAACAATGATTTTCTTGAATGGGCATGAATGGAGATGCCCGCACAGTAATGTAGAAATGTTTCAT ACAGCTATTAAAATGTAACTGACCTCCTTAGAGGCAGATTAGTAACTGTTCCTACTTTGTATAGCTAAGTGACAGTCACTTAACTTACATGACTTTCTTT TTTCACATTGGGTCTCTGGTCCTGTGTCTTCACCTCATTTATAGCACGTCTCCTTGATTTTTGGTAGTATCAACTTCCCAGTGATCTGTTCAGTTAAGTT CTTCTCCCGTTAACCAGGAAGTGCTTATTCTCTCATCACAGTGGGAAGAATAGCCTATTGTCTTTCATTTTGCCTGAGTGTATTTTACTATTTGGGCTCT GAAATAAAAATTATGAAATATGGTGAGGTCACATGTTGGTGCTGCCTTGCTGCATAAAATTCTAGAGGGCAGGTTAGAGACAGTATGTATGCCTTCGGGA AAATTCAAAGGTGGATTACAAGGTGTCCTCAGCATGCCCTATGGCCTATGTGCGAAGCAAGAAGAATTGACTGATTTACAGGACTTCTCTTTATGTCAAT CTTAAGAGGATGGATGAATCTGGACATTTGTTCCACCCGACCTCTGACTGATGGTTTGGAAAATAACTTTAATTAGGATCATATGACCATTGAAAAAGGA AAAATGTAGACTCTGACTTCCGTCCCACTGAAGGATTAATGAAAACCTTTACTAGCATTTAGAGCTTTTCAGAACATCCCCACTGTCATGTGTCTCAGCA GTGGAGACTGCAAGTAAGGCTTTTAATTTTAGGAGGTTTTTTTTTTTTTTTTTTTTTCCCTAAATGGTATGGCCAAAAGTCAGAGTTAAAATATATATAG TTAGATTCCAACTTCCTCCTTCACTCTAAAAATAGAATCCAAACCCACTCTTCATATATGCTTCCAGAATGGGGCTTAAGTACCAATCTCTGCTTTGCAA TGGGCACAATCTTGGTCATGTCCTGAGGCTCTCTAAGAAAAGAGAGGATCTAGGATGGGAGAGCTAGAAAGTTGCTAACTGGGAAGAACAAGGCCCTGAG GGCTTGGTCTACCAATCTGGGAAGATTTGAAAACAAACTTCTCGCAACTGAAGGAAGGCTGAAGGCTGCTGCAAGTCATTGAGTGACTTTAGGATGAGCA AAACATTGGGCCACTTCCTAATGCCCTATGTGTATAGTACCAGAAGCAAGGTCTCAGACTTAACAGACCCAGCTCTGTTCCAAGGTGAGTCTGAACCAAT AGAAAGCAAACATGTGCAGATATCCAAACAAGACTGCTCATGCAAGTCGGGGCTGGCTACCCGTCTTAGGCAGCAACAGCAGAGCTCCAGGGAGCTTATT CAATATTTACTGAGACTTCGAAGACCCAGCAGATGTTTAATGAAGTCACTATTTTGGCTCAAACCCTCCACTTCTCCCCCTCCCCTCAAAAAGCCAACAG GTAAACACATAAATGAAAGAAACCCACAGAAGGGGATGGGAAATAAAGAAAATTCTCTCAAGACTTCTCCAGGCCCATGTCACTGGTCAGCGTGGTTTTT ATGTGTATTAGGATTGGGGGATGTGAAGAAATAAGTATCCAGTACTTTATAACCAAAGCAATTAAATGATATTGGGTAGGGAATGTTGGCCAGTTTTGTT TAGTTTTGCATCACATTGTCACCAGACTCACTAGCCCAAGTAATCGGGCGCCCGAAGAGGGAGACAGAGATGTGCAGAGTTGACCAGTGTGCGGATGATA ACTACTGACGAAAGAGTCATCGACTCAGTTAGTGGTTGGATGTAGTCACATTAGTTTGCCTCTCCCCATCTTTGTCTCCCTGGCAAGGAGAATATGCGGG ACATGATGCTAAGAGTCCTGGGTAAATGTGGTGAGAATGCACGCGTGCATATGCTACACATATGTGCTTCTCAGTTGCAGAAAATGAACTGCTTTGGGAG ATTATCAGTAGAAAGAGTGTTATCATATTGGTGCTGAGTGCTATGTGTGCTTATACAATTTGTTCTTGTATTTTAATAAACTTTGAATAAAAGAATAAAG CTA

refseq_DIO2.F1 (forward primer)
refseq_DIO2.R1 (reverse primer)
Single exon skipping

Frame 0

GCRERHFAPQTDSKKGKTESEKKEESVAPGEGR E*DWEKEKHRKCV*NGVKKEKKKTTLKAHLKKKK TLAIQERNRLRLKSIETMKG*RKF*NLCHSLIG AWK*K*NCLSLTDSDRGNWIRDEYASFFFFFFF FFLTS*ILKKKKKKKKRQQLRIE*IDGHTPTAGL ERLDLVLPFLLL*KRRQLGLPFNLVFFPLLPQP PTFPLTSPTPFITTPLLNKRVKGNQSAQGN*LR RQRRWASSA*TC*SHCKFCQFFSPTASSWLSMTR SFCSSTWCCC*AAPSPLAESGGAC*PQRDCAAS GRASSSMPTNS*IVLHQVLAKMDTFYD*YMKLI KADYWSTHIWIYGW*NWVRMPPIPVWCMSPVQKE VTTVAMVPRRR*LREPHATFLTLPALSAH*WST LAQPLDLLSRASCQPSANWWKSSPQWLTSCWST LMRLIHQMAGRYRGTPLCLLR*RSTRTRKIDVQQ PSSFWSVSPCRPSAELWLTAWTITPT*LTG*PL NVCALCRDRKLLIWEERAPSPTTFKKSGIGWRR ISARDERKLD*LVKGMIIRELIVLKSYIKARKLR TESIFQQSPIGLLKDRSLSIGRT*ISNSSILLS LLKWHWAE*VTISPLFLVKSPM*KDPKMERKKR *FSMCSFCIEKELIHLMHALRPEEKTYLNNYYIR EATVYVKIKGIALALFGMDGAQLENSQILQQVL EPRPCG*TLVLRLDLMLESFLMFQLLAM*CSQS SYPRNYWYICRYRE*SLSLRG*NDHL*SEG*FLG RWREW*KRNEIDKLGKRRQKDLENSQSFTLKL* RVGHIC*PRKHRNIHKARKRRRSSTKSGIENTH IKTIYLSYAPREEKGVIERKKNT*IFVIVRGFFW K*LLLNHVCGMYIFSGLIIPHDTY*RKTSGSGG AGLFLPIPTISMWPKSFLILVSIAVFSLNAELK KLYVHTHIHTYIQIYVYIFSAAAGGRYHGHSAQP *FPPKVGEL**RIGANKQAYFHCKIEEEEVRDN SDQSFWRLCYKATPGISWNFH*HLNLDLDLPLV PLAEV**SKVPIVYDYNAILKNIYIKYFFLFKID TIVLPISNI*RL*LPKEWTNHFRIIISFW*I*D YYEIIVYDCI*RYNQRIFCPFH**LNKK*IKWI EKN*S*KYILKLVV*IEKRVRTRCARTKRILFYF LNGSNISVVSPAGILCKY*SSIGTDFSWQHQLS NVLNSIKTTHKETKQISLSNELLENHIENNYFR FLSC*LLHSRLISAPIDF*PQNGVEIYVEP*SSK IKNLVAAHSHCCP*NSPFFSMYDKVTVCQISRW GDEIRLRQC*STGEKDDGKITQLCYIFKEGGRL CVQTILPEVSPMEK*SRGRKHRKTWAIREEDVQ* NMQEFLEERLWKGQWRK*MDKAQESYAM*NVLV LSGLSL*LCNLFIAT*IFMISCDVFFYEINKNS LF*GRGKSMLYLIC*EIIRLPILMCVSCVL*GLF L*RIVVLSPTGKCVSIYIS*YKSMIYLYHI*KS EFSFLVPNHVSPIGCVYMELSV*PFKLH*LVYY *NSFQEILDIIITSGSTQTLIV*KTYVLDLSKTD FIYCLVKILVGSTMIFLNGHEWRCPHSNVEMFH TAIKM*LTSLEAD**LFLLCIAK*QSLNLHDFL FSHWVSGPVSSPHL*HVSLIFGSINFPVICSVKF FSR*PGSAYSLITVGRIAYCLSFCLSVFYYLGS EIKIMKYGEVTCWCCLAA*NSRGQVRDSMYAFG KIQRWITRCPQHALWPMCEARRID*FTGLLFMSI LRGWMNLDICSTRPLTDGLENNFN*DHMTIEKG KM*TLTSVPLKD**KPLLAFRAFQNIPTVMCLS SGDCK*GF*F*EVFFFFFFSLNGMAKSQS*NIYS *IPTSSFTLKIESKPTLHICFQNGA*VPISALQ WAQSWSCPEAL*EKRGSRMGELESC*LGRTRP* GLGLPIWEDLKTNFSQLKEG*RLLQVIE*L*DEQ NIGPLPNALCV*YQKQGLRLNRPSSVPR*V*TN RKQTCADIQTRLLMQVGAGYPS*AATAELQGAY SIFTETSKTQQMFNEVTILAQTLHFSPSPQKANR *THK*KKPTEGDGK*RKFSQDFSRPMSLVSVVF MCIRIGGCEEISIQYFITKAIK*YWVGNVGQFC LVLHHIVTRLTSPSNRAPEEGDRDVQS*PVCG** LLTKESSTQLVVGCSHISLPLPIFVSLARRICG T*C*ESWVNVVRMHACICYTYVLLSCRK*TALG DYQ*KECYHIGAECYVCLYNLFLYFNKL*IKE*S

Frame 1

AAERGTLHHRQIARRERQRVRKKRSQSLLGKGE SETGRKRSTESVCKTE*RKKKKKLPLKHI*KKK KLWQFKKETGYV*RA*RQ*KAKENFKISATVS*V LGNESRTACL*RTLTEVTGLGTSTPAFFFFFFF FF*HLKS*KKKKKKKKGSSSELNELMGTLQLLG WRDWT*SCHFCFFERGDNLGFLLI*FFFPFSPNP QPSPLPPPPPLSPPPF*IRG*RGTRAHKGTDSG GREDGHPQRRLADHTANSASFFLQLPLPGSL*L GHSAQARGAAVEPLQVHSRRVAAHADLRGTALRL EELPPRCLQTAKLSSIRF*QRWTHFMTSI*SL* KQTTGLLTFGFMDGEIG*GCPQFQCGACLQYRR R*QQWQWYPGEDS*GSHMPPS*LCQP*APTSGQL WLSHLTSFHEPAASLPQTGGRVLLSG*LPAGLH **GSSIRWLGDTGGLLFVF*GEEAPEPGRSMCS SPAASGAFLLAAPVPSCG*PHGQ*RQHSLRGSL* TCVHCAETENCLSGRKGPLLLQPSRSPALAGEE FQQEMKEN*ISWLKV*L*ESLLF*KVI*RQGN* ELNPYFNRALLAY*KTGVYLSEEHESLTAPYFFH YSNGIGLSK*PYHLSS**KALCEKIPRWRGRNA NSACVHSALRRN*YI*CML*DQKKRLT*IITTL GKLLSTLR*RVLPWLYLAWMEPSWKIPKYYNKSL NPGHVVRRWC*G*TLC*SHF*CSSF*PCSALSL HTPEIIGIFVDTENDPSV*EVRMIICNLRVNF* AGGESGKKEMKLTS*ERGGRKIWKIHRVSPLSCR EWVTFVSHGNIETYTRPEKEEGAQLKVA*RIHI *KQYICHMLLERRKG*LKEKKILKYL*L*GVSF GNNYF*TMYVVCIFSVG*LYPMIPIKGKPVGLVV LVFSSPFLQFLCGPSHS*SWSL*QCSL*MLS*R NYTYIHTYIHTYKYMYIYSQLLREVGTMAIQHS LDFLPK*VSYSEE*VQTNKLTSIAK*KKRKLEII LINHFGGFVIRQPPVYHGISIDI*IWTWIFPWS H*LRFSNLKSL*YMIIMLF*KIYI*NIFFFLK* TL*FYP*VIFKDYSSQKNGPTTFVS*FLFGKYET IMKS*YMIVFKGTIKGSFVHSINN*IKNK*NG* KKTKVENTFLN*LSELRKE*ELGVQEPNVFYFI F*MGATYQSCHQLVYCVNIKAPLGLIFHGNISFL MF*IL*KPPTKKQSKFHYLMSCWKIILRIIISD SSVVNFYIQGLSLPPLIFNLKMVLRFTWNPKAV K*KTWLQHIHTVVLKIPLFSLCTIK*QYVR*AGG GMRLG*GSASQLGKRMMEKSPSCAIFLKKEVVY VCRQFSLRLAQWRNEAEEGNIERHGLSGRKMFN RTCKNFWKKGCGRANGENEWTKLRNPTLCRMSWC YQG*ACNYVTYLSQHEFL*FLVMYSFMKLTRTH YFEVEENQCFI*YAEKLLDCQYSCAFHVFYKVC SFEEL*FLVPQGNVYLFIYHSINL*YIYIIYKSL SSLS*SLIMFLP*AVFTWSYRFSLLSFISLSII EIVSKKF*ILS*HLGLLKHLLFERLMSWTYQKL TLFIA**KY*WDQQ*FS*MGMNGDARTVM*KCFI QLLKCN*PP*RQISNCSYFV*LSDSHLTYMTFF FHIGSLVLCLHLIYSTSP*FLVVSTSQ*SVQLS SSPVNQEVLILSSQWEE*PIVFHFA*VYFTIWAL K*KL*NMVRSHVGAALLHKILEGRLETVCMPSG KFKGGLQGVLSMPYGLCAKQEELTDLQDFSLCQ S*EDG*IWTFVPPDL*LMVWKITLIRII*PLKKE KCRL*LPSH*RINENLY*HLELFRTSPLSCVSA VETASKAFNFRRFFFFFFFP*MVWPKVRVKIYI VRFQLPPSL*K*NPNPLFIYASRMGLKYQSLLCN GHNLGHVLRLSKKREDLGWES*KVANWEEQGPE GLVYQSGKI*KQTSRN*RKAEGCCKSLSDFRMS KTLGHFLMPYVYSTRSKVSDLTDPALFQGESEPI ESKHVQISKQDCSCKSGLATRLRQQQQSSRELI QYLLRLRRPSRCLMKSLFWLKPSTSPPPLKKPT GKHINERNPQKGMGNKENSLKTSPGPCHWSAWFL CVLGLGDVKK*VSSTL*PKQLNDIG*GMLASFV *FCITLSPDSLAQVIGRPKRETEMCRVDQCADD NY*RKSHRLS*WLDVVTLVCLSPSLSPWQGEYAG HDAKSPG*MW*ECTRAYATHMCFSVAENELLWE IISRKSVIILVLSAMCAYTICSCILINFE*KNK A

Frame 2

LQREALCTTDR*QEGKDRE*EKRGVSRSWGRER VRLGEREAQKVCVKRSKERKKKNYP*STFKKKK NSGNSRKKQATFKEHRDNERLKKILKSLPQSHR CLEMKVELPVFNGL*QR*LD*GRVRQLFFFFFFF FFNILNPEKKKKKKKKAAAPN*MN*WAHSNCWA GETGLSLAISASLKEETTWASF*FSFFSPSPPT PNLPPYLPHPLYHHPPFK*EGEGEPERTRELTQE AEKMGILSVDLLITLQILPVFFSNCLFLALYDS VILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCV WKSFLLDAYKQLNCPPSGFSKDGHIL*LVYEAYK SRLLVYSHLDLWMVKLGEDAPNSSVVHVSSTEG GDNSGNGTQEKIAEGATCHLLDFASPERPLVVN FGSAT*PPFTSQLPAFRKLVEEFSSVADFLLVYI DEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAA AQQLLERFSLPPQCRVVADRMDNNANIAYGVAF ERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKN FSKR*KKTRLAG*RYDYKRAYCFKKLYKGKEIK N*IHISTEPYWLTERQEFIYRKNMNL*QLHTSF TTQMALG*VSNHITSLLSKKPYVKRSQDGEEETL IQHVFILH*EGTDTSDACFETRRKDLPE*LLH* GSYCLR*DKGYCLGSIWHGWSPVGKFPNITTSP *TQAMWLDVGVKVRPYVRVISDVPASSHVVLSVF IPQKLLVYL*IPRMIPQSERLE*SSVI*GLISR QVERVVKKK*N*QARKEEAERFGKFTEFHP*AV ESGSHLLATET*KHTQGQKKKKELN*KWHREYTY KNNIFVICS*RGERGD*KKKKYLNICNCEGFLL EIITFEPCMWYVYFQWVNYTP*YLLKENQWVWW CWSFPPHSYNFYVAQVIPNLGLYSSVLSEC*AEE IIRTYTHTYIHTNICIYILSCCGR*VPWPFSTA LISSQSR*AIVKNRCKQTSLLPLQNRRRGS*R* F*SIILEALL*GNPRYIMEFPLTFEFGLGSSLGP IS*GLVI*SPYSI*L*CYFKKYIYKIFFSF*NR HYSFTHK*YLKIIAPKRMDQPLSYHNFFLVNMR LL*NHSI*LYLKVQSKDLLSIPLITE*KINKMDR KKLKLKIHS*TSCLN*EKSEN*VCKNQTYFILF FKWEQHISRVTSWYIV*ILKLHWD*FFMATSAF *CSKFYKNHPQRNKANFII**VAGKSY*E*LFQI PQLLTSTFKAYLCPH*FLTSKWC*DLRGTLKQ* NKKPGCSTFTLLSLKFPFFLYVR*SNSMSDKPV GG*D*AEAVLVNWGKG*WKNHPVVLYF*RRRSFM CADNSP*G*PNGEMKQRKET*KDMGYQGGRCSI EHARISGRKAVEGPMEKMNGQSSGILRYVECLG VIRVKPVIM*PIYRNMNFYDFL*CILL*N*QELI ILR*RKINALSDMLRNY*IANTHVRFMCFIRFV PLKNCSS*SHREMCIYLYIIV*IYDIFISYIKV *VLFLSP*SCFSHRLCLHGAIGLAF*ASLACLLL K*FPRNFRYYHNIWVYSNTYCLKDLCLGPIKN* LYLLLSENTSGINNDFLEWA*MEMPAQ*CRNVS YSY*NVTDLLRGRLVTVPTLYS*VTVT*LT*LSF FTLGLWSCVFTSFIARLLDFW*YQLPSDLFS*V LLPLTRKCLFSHHSGKNSLLSFILPECILLFGL *NKNYEIW*GHMLVLPCCIKF*RAG*RQYVCLRE NSKVDYKVSSACPMAYVRSKKN*LIYRTSLYVN LKRMDESGHLFHPTSD*WFGK*L*LGSYDH*KR KNVDSDFRPTEGLMKTFTSI*SFSEHPHCHVSQQ WRLQVRLLILGGFFFFFFFPKWYGQKSELKYI* LDSNFLLHSKNRIQTHSSYMLPEWGLSTNLCFA MGTILVMS*GSLRKERI*DGRARKLLTGKNKALR AWSTNLGRFENKLLATEGRLKAAASH*VTLG*A KHWATS*CPMCIVPEARSQT*QTQLCSKVSLNQ *KANMCRYPNKTAHASRGWLPVLGSNSRAPGSLF NIY*DFEDPADV**SHYFGSNPPLLPLPSKSQQ VNT*MKETHRRGWEIKKILSRLLQAHVTGQRGF YVY*DWGM*RNKYPVLYNQSN*MILGRECWPVLF SFASHCHQTH*PK*SGARRGRQRCAELTSVRMI TTDERVIDSVSGWM*SH*FASPHLCLPGKENMR DMMLRVLGKCGENARVHMLHICASQLQKMNCFGR LSVERVLSYWC*VLCVLIQFVLVF**TLNKRIK L