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Sequence NM_001039715
GAGCTTACTCTGGCTTTGCCGAGATGGGGCTGCCCCAGCCAGGGCTGTGGCTGAAGAGGCTCTGGGTGCTCTTGGAGGTGGCTGTGCATGTGGTCGTGGGTAAAGTGCTTCTGATATTGTTTCCAGACAGAGTCAAGCGGAACATCCTGGCCATGGGCGAGAAGACGGGTATGACCAGGAACCCCCATTTCAGCCACGAC
AACTGGATACCAACCTTTTTCAGCACCCAGTATTTCTGGTTCGTCTTGAAGGTCCGTTGGCAGCGACTAGAGGACACGACTGAGCTAGGGGGTCTGGCCC
CAAACTGCCCGGTGGTCCGCCTCTCAGGACAGAGGTGCAACATTTGGGAGTTTATGCAAGATGGCTGGGCTTTTAAGAACAACATGGACATCAGAAATCA
CCAGAACCTTCAGGATCGCCTGCAGGCAGCCCATCTACTGCTGGCCAGGAGCCCCCAGTGCCCTGTGGTGGTGGACACCATGCAGAACCAGAGCAGCCAG
CTCTACGCAGCACTGCCTGAGAGGCTCTACATAATCCAGGAGGGCAGGATCCTCTACAAGGGTAAATCTGGCCCTTGGAACTACAACCCAGAGGAAGTTC
GTGCTGTTCTGGAAAAGCTCCACAGTTAATCTGGACAGATACCTCAATTCTAGGTGACCAACGGGAGGGCTTCTCAAGGCTTAGCTCTCCCTGAGACCCA
GCTGGCTTTTACCCTTGACCTGTGTCCCTAGCTGAATCACTAGCTCAGATTTTTCTGATCTAAGCAAACAACTCCCAGCTGAGGAATGCAGGCCACAGCA
CCCAATCAAGACAAATTGTTATTATCAGAAAATGAAGCAACACTTGAGCTGTTCAGGCCAGTTCCCTGTTGAAGAAACAGTTCCCTGTTGAAGAAAGTAG
AGCCTGACACTGCTCCCACTTTGGAGACCACATTCCCTGCACACGGTCTTTGAGAGAGCAGTTGCACTCTACAGGCACACTTCTGAGGTACGGTATCTCT
CTCCAGCCACTCTGATACCAAGTAATTCAAGCTGGCATTCCTTCTATTAGGGAAATTCATTTTACCCAATTTGCATTTATGGAATTGATCATTTAAGACA
CTAAATTAGTTTTTAGAACCAATTATGGGAAGAATTCCAGTTGTTAGGAAGAGATGAGGAGTTGGAAGAGGAGGGATTAGAAACAGGAGGAGGCAGTCAT
CCTCTCCTTGCCAAAAGATTTAAACCTGTCCACATTGGTGGTGATGATGGGTGAGTTTCCATGGTAACACATCCCTAATTTTACCAGGGAAGAGGAGAGT
ACTCACTTTACCATCTTTGAATATATTTCATAGAAATCTAGCTCTCTGTACCCTGAAATCTTCCACTAGCCTCACTTTTCAACAGAGTCATCTAGAAGGG
AGGGTTGGCTTCCCAAAAGCATAACCTTGACCAAACCAAACAATAGGCACCAGCAATGCTGTCATTCAGTTATGCAGAAGCTCATTTGTGAAATTCTGTT
TCTCTGATTTCTTCGCAAGTCTCTTAATGGTCATTTGTGTTAGATTACATCAAACTGATGGATAGCCATTGGTATTCATCTATTTTAACTCTGTGTCTTT
ACATATTTGTTTATGATGGCCACAGCCTAAAGTACACACGGCTGTGACTTGATTCAAAAGAAAATGTTATAAGATGCAGTAAACTAATAACAGAATTATT
AAAATATATCAGGCTA
- refseq_DIO1.F1 (forward primer)
- refseq_DIO1.R1 (reverse primer)
- Single exon skipping
Frame 0
ELTLALPRWGCPSQGCG*RGSGCSWRWLCMWSWVKCF*YCFQTESSGTSWPWARRRV*PGTPISATT
TGYQPFSAPSISGSS*RSVGSD*RTRLS*GVWP
QTARWSASQDRGATFGSLCKMAGLLRTTWTSEI
TRTFRIACRQPIYCWPGAPSALWWWTPCRTRAAS
STQHCLRGST*SRRAGSSTRVNLALGTTTQRKF
VLFWKSSTVNLDRYLNSR*PTGGLLKA*LSLRP
SWLLPLTCVPS*ITSSDFSDLSKQLPAEECRPQH
PIKTNCYYQKMKQHLSCSGQFPVEETVPC*RK*
SLTLLPLWRPHSLHTVFERAVALYRHTSEVRYL
SPATLIPSNSSWHSFY*GNSFYPICIYGIDHLRH
*ISF*NQLWEEFQLLGRDEELEEEGLETGGGSH
PLLAKRFKPVHIGGDDG*VSMVTHP*FYQGRGE
YSLYHL*IYFIEI*LSVP*NLPLASLFNRVI*KG
GLASQKHNLDQTKQ*APAMLSFSYAEAHL*NSV
SLISSQVS*WSFVLDYIKLMDSHWYSSILTLCL
YIFVYDGHSLKYTRL*LDSKENVIRCSKLITELL
KYIRL
Frame 1
SLLWLCRDGAAPARAVAEEALGALGGGCACGRG*SASDIVSRQSQAEHPGHGREDGYDQEPPFQPR
QLDTNLFQHPVFLVRLEGPLAATRGHD*ARGSGP
KLPGGPPLRTEVQHLGVYARWLGF*EQHGHQKS
PEPSGSPAGSPSTAGQEPPVPCGGGHHAEPEQP
ALRSTA*EALHNPGGQDPLQG*IWPLELQPRGSS
CCSGKAPQLIWTDTSILGDQREGFSRLSSP*DP
AGFYP*PVSLAESLAQIFLI*ANNSQLRNAGHS
TQSRQIVIIRK*SNT*AVQASSLLKKQFPVEESR
A*HCSHFGDHIPCTRSLREQLHSTGTLLRYGIS
LQPL*YQVIQAGIPSIREIHFTQFAFMELII*D
TKLVFRTNYGKNSSC*EEMRSWKRRD*KQEEAVI
LSLPKDLNLSTLVVMMGEFPW*HIPNFTREEES
THFTIFEYIS*KSSSLYPEIFH*PHFSTESSRR
EGWLPKSITLTKPNNRHQQCCHSVMQKLICEILF
L*FLRKSLNGHLC*ITSN*WIAIGIHLF*LCVF
TYLFMMATA*STHGCDLIQKKML*DAVN**QNY
*NISG
Frame 2
AYSGFAEMGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHD
NWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLA
PNCPVVRLSGQRCNIWEFMQDGWAFKNNMDIRNH
QNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQ
LYAALPERLYIIQEGRILYKGKSGPWNYNPEEV
RAVLEKLHS*SGQIPQF*VTNGRASQGLALPETQ
LAFTLDLCP*LNH*LRFF*SKQTTPS*GMQATA
PNQDKLLLSENEATLELFRPVPC*RNSSLLKKV
EPDTAPTLETTFPAHGL*ESSCTLQAHF*GTVSL
SSHSDTK*FKLAFLLLGKFILPNLHLWN*SFKT
LN*FLEPIMGRIPVVRKR*GVGRGGIRNRRRQS
SSPCQKI*TCPHWW**WVSFHGNTSLILPGKRRV
LTLPSLNIFHRNLALCTLKSSTSLTFQQSHLEG
RVGFPKA*P*PNQTIGTSNAVIQLCRSSFVKFC
FSDFFASLLMVICVRLHQTDG*PLVFIYFNSVSL
HICL*WPQPKVHTAVT*FKRKCYKMQ*TNNRII
KIYQA
Sequence NM_000792
GAGCTTACTCTGGCTTTGCCGAGATGGGGCTGCCCCAGCCAGGGCTGTGGCTGAAGAGGCTCTGGGTGCTCTTGGAGGTGGCTGTGCATGTGGTCGTGGGTAAAGTGCTTCTGATATTGTTTCCAGACAGAGTCAAGCGGAACATCCTGGCCATGGGCGAGAAGACGGGTATGACCAGGAACCCCCATTTCAGCCACGAC
AACTGGATACCAACCTTTTTCAGCACCCAGTATTTCTGGTTCGTCTTGAAGGTCCGTTGGCAGCGACTAGAGGACACGACTGAGCTAGGGGGTCTGGCCC
CAAACTGCCCGGTGGTCCGCCTCTCAGGACAGAGGTGCAACATTTGGGAGTTTATGCAAGGTAATAGGCCACTGGTGCTGAATTTTGGAAGTTGTACCTG
ACCTTCATTTATGTTCAAATTTGACCAGTTCAAGAGGCTTATTGAAGACTTTAGTTCCATAGCAGATTTTCTTGTCATTTACATTGAAGAAGCACATGCA
TCAGATGGCTGGGCTTTTAAGAACAACATGGACATCAGAAATCACCAGAACCTTCAGGATCGCCTGCAGGCAGCCCATCTACTGCTGGCCAGGAGCCCCC
AGTGCCCTGTGGTGGTGGACACCATGCAGAACCAGAGCAGCCAGCTCTACGCAGCACTGCCTGAGAGGCTCTACATAATCCAGGAGGGCAGGATCCTCTA
CAAGGGTAAATCTGGCCCTTGGAACTACAACCCAGAGGAAGTTCGTGCTGTTCTGGAAAAGCTCCACAGTTAATCTGGACAGATACCTCAATTCTAGGTG
ACCAACGGGAGGGCTTCTCAAGGCTTAGCTCTCCCTGAGACCCAGCTGGCTTTTACCCTTGACCTGTGTCCCTAGCTGAATCACTAGCTCAGATTTTTCT
GATCTAAGCAAACAACTCCCAGCTGAGGAATGCAGGCCACAGCACCCAATCAAGACAAATTGTTATTATCAGAAAATGAAGCAACACTTGAGCTGTTCAG
GCCAGTTCCCTGTTGAAGAAACAGTTCCCTGTTGAAGAAAGTAGAGCCTGACACTGCTCCCACTTTGGAGACCACATTCCCTGCACACGGTCTTTGAGAG
AGCAGTTGCACTCTACAGGCACACTTCTGAGGTACGGTATCTCTCTCCAGCCACTCTGATACCAAGTAATTCAAGCTGGCATTCCTTCTATTAGGGAAAT
TCATTTTACCCAATTTGCATTTATGGAATTGATCATTTAAGACACTAAATTAGTTTTTAGAACCAATTATGGGAAGAATTCCAGTTGTTAGGAAGAGATG
AGGAGTTGGAAGAGGAGGGATTAGAAACAGGAGGAGGCAGTCATCCTCTCCTTGCCAAAAGATTTAAACCTGTCCACATTGGTGGTGATGATGGGTGAGT
TTCCATGGTAACACATCCCTAATTTTACCAGGGAAGAGGAGAGTACTCACTTTACCATCTTTGAATATATTTCATAGAAATCTAGCTCTCTGTACCCTGA
AATCTTCCACTAGCCTCACTTTTCAACAGAGTCATCTAGAAGGGAGGGTTGGCTTCCCAAAAGCATAACCTTGACCAAACCAAACAATAGGCACCAGCAA
TGCTGTCATTCAGTTATGCAGAAGCTCATTTGTGAAATTCTGTTTCTCTGATTTCTTCGCAAGTCTCTTAATGGTCATTTGTGTTAGATTACATCAAACT
GATGGATAGCCATTGGTATTCATCTATTTTAACTCTGTGTCTTTACATATTTGTTTATGATGGCCACAGCCTAAAGTACACACGGCTGTGACTTGATTCA
AAAGAAAATGTTATAAGATGCAGTAAACTAATAACAGAATTATTAAAATATATCAGGCTA
- refseq_DIO1.F1 (forward primer)
- refseq_DIO1.R1 (reverse primer)
- Single exon skipping
Frame 0
ELTLALPRWGCPSQGCG*RGSGCSWRWLCMWSWVKCF*YCFQTESSGTSWPWARRRV*PGTPISATT
TGYQPFSAPSISGSS*RSVGSD*RTRLS*GVWP
QTARWSASQDRGATFGSLCKVIGHWC*ILEVVP
DLHLCSNLTSSRGLLKTLVP*QIFLSFTLKKHMH
QMAGLLRTTWTSEITRTFRIACRQPIYCWPGAP
SALWWWTPCRTRAASSTQHCLRGST*SRRAGSS
TRVNLALGTTTQRKFVLFWKSSTVNLDRYLNSR*
PTGGLLKA*LSLRPSWLLPLTCVPS*ITSSDFS
DLSKQLPAEECRPQHPIKTNCYYQKMKQHLSCS
GQFPVEETVPC*RK*SLTLLPLWRPHSLHTVFER
AVALYRHTSEVRYLSPATLIPSNSSWHSFY*GN
SFYPICIYGIDHLRH*ISF*NQLWEEFQLLGRD
EELEEEGLETGGGSHPLLAKRFKPVHIGGDDG*V
SMVTHP*FYQGRGEYSLYHL*IYFIEI*LSVP*
NLPLASLFNRVI*KGGLASQKHNLDQTKQ*APA
MLSFSYAEAHL*NSVSLISSQVS*WSFVLDYIKL
MDSHWYSSILTLCLYIFVYDGHSLKYTRL*LDS
KENVIRCSKLITELLKYIRL
Frame 1
SLLWLCRDGAAPARAVAEEALGALGGGCACGRG*SASDIVSRQSQAEHPGHGREDGYDQEPPFQPR
QLDTNLFQHPVFLVRLEGPLAATRGHD*ARGSGP
KLPGGPPLRTEVQHLGVYAR**ATGAEFWKLYL
TFIYVQI*PVQEAY*RL*FHSRFSCHLH*RSTC
IRWLGF*EQHGHQKSPEPSGSPAGSPSTAGQEPP
VPCGGGHHAEPEQPALRSTA*EALHNPGGQDPL
QG*IWPLELQPRGSSCCSGKAPQLIWTDTSILG
DQREGFSRLSSP*DPAGFYP*PVSLAESLAQIFL
I*ANNSQLRNAGHSTQSRQIVIIRK*SNT*AVQ
ASSLLKKQFPVEESRA*HCSHFGDHIPCTRSLR
EQLHSTGTLLRYGISLQPL*YQVIQAGIPSIREI
HFTQFAFMELII*DTKLVFRTNYGKNSSC*EEM
RSWKRRD*KQEEAVILSLPKDLNLSTLVVMMGE
FPW*HIPNFTREEESTHFTIFEYIS*KSSSLYPE
IFH*PHFSTESSRREGWLPKSITLTKPNNRHQQ
CCHSVMQKLICEILFL*FLRKSLNGHLC*ITSN
*WIAIGIHLF*LCVFTYLFMMATA*STHGCDLIQ
KKML*DAVN**QNY*NISG
Frame 2
AYSGFAEMGLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHD
NWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLA
PNCPVVRLSGQRCNIWEFMQGNRPLVLNFGSCT*
PSFMFKFDQFKRLIEDFSSIADFLVIYIEEAHA
SDGWAFKNNMDIRNHQNLQDRLQAAHLLLARSP
QCPVVVDTMQNQSSQLYAALPERLYIIQEGRILY
KGKSGPWNYNPEEVRAVLEKLHS*SGQIPQF*V
TNGRASQGLALPETQLAFTLDLCP*LNH*LRFF
*SKQTTPS*GMQATAPNQDKLLLSENEATLELFR
PVPC*RNSSLLKKVEPDTAPTLETTFPAHGL*E
SSCTLQAHF*GTVSLSSHSDTK*FKLAFLLLGK
FILPNLHLWN*SFKTLN*FLEPIMGRIPVVRKR*
GVGRGGIRNRRRQSSSPCQKI*TCPHWW**WVS
FHGNTSLILPGKRRVLTLPSLNIFHRNLALCTL
KSSTSLTFQQSHLEGRVGFPKA*P*PNQTIGTSN
AVIQLCRSSFVKFCFSDFFASLLMVICVRLHQT
DG*PLVFIYFNSVSLHICL*WPQPKVHTAVT*F
KRKCYKMQ*TNNRIIKIYQA
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