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Sequences

Sequence NM_021131

GGAGGAAGGAGGAGGTCACCGTCCAGCTGTCTCTCCCCTGTCCCCACATGTCTTCTAAGCTGTTGGAGCCGGCCGGCGCTCACTTGTCTTCAGGAAGCTC GGAGCCTTTGGTGGAGCCGGGGAGAGGAAGGGTGGGTGCAAGAGTGAAAGGCGAGAGGGGACTGCAAGCATCCGGGTCGGCTCCTGGCCGGAGCAAGATG GCTGAGGGCGAGCGGCAGCCGCCGCCAGATTCTTCAGAGGAGGCCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAG ACATGGGCAAATGGAAGCGTTCTCAGGCATACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTA CAGAGTCTCCGAGGCCATTGAGAAACTAGTCGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGG AATAAGGCATACAGGACCTGGTATGCCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGG TGGCTGTTTACCTAAAGGAGTCAGTGGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTCTCTGCAA GATTGGGGTGCTCCGGGTGGATGACCAAATAGCTATTGTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATG GAGCCAGCCGGCAGCCAGGGAGTGTGGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGC CCAGACACTTTGTGGATGAGAAGGCCGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACTGGCCCATT TGCAGAGCACTCCAACCAGCTGTGGAACATCAGCGCCGTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAG AAGTTCCCTGTGATCCAGCACTTCAAGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAGGAGGGGCCAAGCCGAAGAGCCACCCAGGCCA CAGTTCCTGTGCCTGCCTTCCCCACCCCAGCAGTGGCCCCTCCCCATCCCCTCCCTCTGTTCGTCCCGTTTGATGAGAGGCTGTTTACTGGGGTGGGGTG GCGAGATGGGCTTGAGGGGGCTCAGAGCATAAGGCTTCAGGGCCCAAGTTGGGAGAAGTGACCAAAGTGTAGCCAGTTTTCTGAGTTCCCGTGTGCTAGA CTGGCCAGAAGAGAGGGTCTGGGGCCTGGTCACTCGGCCACTCTCTCCTGTTTCTGGCCTCTTCTCCCTTCACTCCCGTCCAGTCTGGTTTTGAGAGCAG GGGCTGTTCTGCAGCACCGCAGGGAAGGGAGGAGAGATACCTGCTGCTTCCATTGCTTTTCCCTTCCTGGAGTCGATGCCTTTCTAAGGGTTGGAGCTGC TCCTTGCAGGGGCGGGTCAGTTTCCCAGGCCATGCCGGGGTGGCCATCTATGGTAGGGCTGGAAGCTGAGGCTGGCCGCCAGCTGTGGGCTGGGGTGGGG TGGGTGGGGTCGGGTGGTGGAGAGGCCTTAGCTGTCCTGGCTGGTGCCCCTCCCAGGCTCCTTTTCACCCTGCCCCCTGGGCCTGAGGCCCCCTGTGTCC AAGCCTCCCCCTGGCTCTTCAGTTCTCTAGCCCTTGGCTCTGCTGGGTTTCCTGACTGTAGCCACATCTCTCCCGCTCCCTAAGGGTAACCTAGCCAATG GAAGCTGCCCTTTGGGTAGGTGCTGGGCTCCTGGGAGGGCCCAGATGATGGGGTGAGGCATGTCTTTCCAGAACTTTCCCTGGCAGGGAGGGGATGGCAG AAACTCAGGGAGGGGCTTGGGGCCCATTGTATCTGGAGAGCCTGGATTCCTCTTGGCAGTCTTAGGCCCGGCCACTTCTGCTACCTTTGCGCTGCTGTGA GCCTCACCCTGGGCCCCTGGGCCCTGCTTCTCTGCTCCCCTGGGTGATGGGTGGGCCCAGAAGGTGGCAGTCCCACACCTTGTCCTCCCACCTCCCTGAA CTGTCCATTGCTTTTATAGGGTGAGGTAAGAGACAGCCTCCCAAGCCCAGGCTTTGGCACTCAGAATGGGCCCAGTGGGGGCTGGGCAGGCCCATTGAGG GCCACCGCCGAGGTTTCTCCTAGGGCTGTTCCTGGGCCTGGCTCTTACAGGCTCGTCCCCCAGGCCTGCCCTTCTCCACTGCCCCCTCCTGTGTCTGGGT CCACACACCCTTCAGGAAGGGGGAGCACTGAGAAGCACAGCACAGGGGCTCAGCCTGGGATCCGGTGATGGTCTGGGCAGAGGCTGGGTCAGGAGTCCCA AAGGTCAGTGACAGTTTCTCAGAAGAGGCCCAGCGTCCACCTCTCTCCCAGGGCCAGACAGCCCTTCCTGGCTCCCCCATCCCCCTATGGGCTCCCAGCC CCTTGCACCCTCATTGCTGTTCAGATTAAAGCCTCTGTTTTGCACCTGT

refseq_PPP2R4.F1 (forward primer)
refseq_PPP2R4.R1 (reverse primer)
Single exon skipping

Frame 0

GGRRRSPSSCLSPVPTCLLSCWSRPALTCLQEA RSLWWSRGEEGWVQE*KARGDCKHPGRLLAGARW LRASGSRRQILQRRPLQPLRTSSFQKRRSTQFQ TWANGSVLRHTLTTSDSSLPSTKV*RGRS*PSS TESPRPLRN*SLFSTRWTGGLMRLLQWTSPLGLG IRHTGPGMPNLMRKQKTWWPQWSLPIWQLLCLR WLFT*RSQWGTPRALTTAQGMRQPSLLSSAVSA RLGCSGWMTK*LLSSRCSIGTLRLCGNSRKHTGW SQPAARECGVWMTSSFCPSSGAVRS**TTHTWS PDTLWMRRP*MRTTRTTCSWSVSCLLPR*RLAH LQSTPTSCGTSAPSLPGPK*TRVSSACIRPSAWR SSL*SSTSSSGACCPSILSRRARRGQAEEPPRP QFLCLPSPPQQWPLPIPSLCSSRLMRGCLLGWG GEMGLRGLRA*GFRAQVGRSDQSVASFLSSRVLD WPEERVWGLVTRPLSPVSGLFSLHSRPVWF*EQ GLFCSTAGKGGEIPAASIAFPFLESMPF*GLEL LLAGAGQFPRPCRGGHLW*GWKLRLAASCGLGWG GWGRVVERP*LSWLVPLPGSFSPCPLGLRPPVS KPPPGSSVL*PLALLGFLTVATSLPLPKGNLAN GSCPLGRCWAPGRAQMMG*GMSFQNFPWQGGDGR NSGRGLGPIVSGEPGFLLAVLGPATSATFALL* ASPWAPGPCFSAPLGDGWAQKVAVPHLVLPPP* TVHCFYRVR*ETASQAQALALRMGPVGAGQAH*G PPPRFLLGLFLGLALTGSSPRPALLHCPLLCLG PHTLQEGGALRSTAQGLSLGSGDGLGRGWVRSP KGQ*QFLRRGPASTSLPGPDSPSWLPHPPMGSQP LAPSLLFRLKPLFCTC

Frame 1

EEGGGHRPAVSPLSPHVF*AVGAGRRSLVFRKL GAFGGAGERKGGCKSERREGTASIRVGSWPEQD G*GRAAAAARFFRGGPSSHSELHHSKKGDPHSSR HGQMEAFSGIR*LHRIHPYPQRRCEGEEADLRV QSLRGH*ETSRSSQHAGQVD**DSSSGPALSVW E*GIQDLVCQT**GSRKLGGHSGPYPSGSCCA*G GCLPKGVSGELHAH*LRHRA*GSLRCFPLLSLQ DWGAPGG*PNSYCLQGVQSVP*GYAETPENIQD GASRQPGSVGSG*LPVSALHLGQFAADRPPIPGA QTLCG*EGRE*EPQGLHVPGVYPVYYRDEDWPI CRALQPAVEHQRRPFLVQSEPGSHPHV*GRVPG EVPCDPALQVREPAAHPSCHVGLGGAKPKSHPGH SSCACLPHPSSGPSPSPPSVRPV**EAVYWGGV ARWA*GGSEHKASGPKLGEVTKV*PVF*VPVC* TGQKRGSGAWSLGHSLLFLASSPFTPVQSGFESR GCSAAPQGREERYLLLPLLFPSWSRCLSKGWSC SLQGRVSFPGHAGVAIYGRAGS*GWPPAVGWGG VGGVGWWRGLSCPGWCPSQAPFHPAPWA*GPLCP SLPLALQFSSPWLCWVS*L*PHLSRSLRVT*PM EAALWVGAGLLGGPR*WGEACLSRTFPGREGMA ETQGGAWGPLYLESLDSSWQS*ARPLLLPLRCCE PHPGPLGPASLLPWVMGGPRRWQSHTLSSHLPE LSIAFIG*GKRQPPKPRLWHSEWAQWGLGRPIE GHRRGFS*GCSWAWLLQARPPGLPFSTAPSCVWV HTPFRKGEH*EAQHRGSAWDPVMVWAEAGSGVP KVSDSFSEEAQRPPLSQGQTALPGSPIPLWAPS PLHPHCCSD*SLCFAP

Frame 2

RKEEVTVQLSLPCPHMSSKLLEPAGAHLSSGSS EPLVEPGRGRVGARVKGERGLQASGSAPGRSKM AEGERQPPPDSSEEAPPATQNFIIPKKEIHTVP DMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEY RVSEAIEKLVALLNTLDRWIDETPPVDQPSRFG NKAYRTWYAKLDEEAENLVATVVPTHLAAAVPE VAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCK IGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRM EPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLE PRHFVDEKAVNENHKDYMFLECILFITEMKTGPF AEHSNQLWNISAVPSWSKVNQGLIRMYKAECLE KFPVIQHFKFGSLLPIHPVTSG*EGPSRRATQA TVPVPAFPTPAVAPPHPLPLFVPFDERLFTGVGW RDGLEGAQSIRLQGPSWEK*PKCSQFSEFPCAR LARREGLGPGHSATLSCFWPLLPSLPSSLVLRA GAVLQHRREGRRDTCCFHCFSLPGVDAFLRVGAA PCRGGSVSQAMPGWPSMVGLEAEAGRQLWAGVG WVGSGGGEALAVLAGAPPRLLFTLPPGPEAPCV QASPWLFSSLALGSAGFPDCSHISPAP*G*PSQW KLPFG*VLGSWEGPDDGVRHVFPELSLAGRGWQ KLREGLGAHCIWRAWIPLGSLRPGHFCYLCAAV SLTLGPWALLLCSPG*WVGPEGGSPTPCPPTSLN CPLLL*GEVRDSLPSPGFGTQNGPSGGWAGPLR ATAEVSPRAVPGPGSYRLVPQACPSPLPPPVSG STHPSGRGSTEKHSTGAQPGIR*WSGQRLGQESQ RSVTVSQKRPSVHLSPRARQPFLAPPSPYGLPA PCTLIAVQIKASVLHL

Sequence NM_178000

CCGCACTGACATGGCCGTCGCCCGGTTCCGCGCGTCCGCCGCGCGCCGGCCGTTAATAGGCTTGCTCCCTGAGCGCCCCGCACCGACATGGCGGCCGTCT TCGCTGTGGTGACTTTAACTCTCGGTTTTCGGTTATAGCCGGCCGGCGCTCACTTGTCTTCAGGAAGCTCGGAGCCTTTGGTGGAGCCGGGGAGAGGAAG GGTGGGTGCAAGAGTGAAAGGCGAGAGGGGACTGCAAGCATCCGGGTCGGCTCCTGGCCGGAGCAAGATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGAT TCTTCAGAGGAGGCCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAGCGTTCTCAGGCAT ACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGGCCATTGAGAAACTAGT CGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATACAGGACCTGGTATGCCAAA CTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGTGGGGA ACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGACCAAAT AGCTATTGTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGCAGCCAGGGAGTGTGGGGT CTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGCCGTGA ATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACTGGCCCATTTGCAGAGCACTCCAACCAGCTGTGGAACAT CAGCGCCGTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTGATCCAGCACTTCAAGTTC GGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAGGAGGGGCCAAGCCGAAGAGCCACCCAGGCCACAGTTCCTGTGCCTGCCTTCCCCACCCCAG CAGTGGCCCCTCCCCATCCCCTCCCTCTGTTCGTCCCGTTTGATGAGAGGCTGTTTACTGGGGTGGGGTGGCGAGATGGGCTTGAGGGGGCTCAGAGCAT AAGGCTTCAGGGCCCAAGTTGGGAGAAGTGACCAAAGTGTAGCCAGTTTTCTGAGTTCCCGTGTGCTAGACTGGCCAGAAGAGAGGGTCTGGGGCCTGGT CACTCGGCCACTCTCTCCTGTTTCTGGCCTCTTCTCCCTTCACTCCCGTCCAGTCTGGTTTTGAGAGCAGGGGCTGTTCTGCAGCACCGCAGGGAAGGGA GGAGAGATACCTGCTGCTTCCATTGCTTTTCCCTTCCTGGAGTCGATGCCTTTCTAAGGGTTGGAGCTGCTCCTTGCAGGGGCGGGTCAGTTTCCCAGGC CATGCCGGGGTGGCCATCTATGGTAGGGCTGGAAGCTGAGGCTGGCCGCCAGCTGTGGGCTGGGGTGGGGTGGGTGGGGTCGGGTGGTGGAGAGGCCTTA GCTGTCCTGGCTGGTGCCCCTCCCAGGCTCCTTTTCACCCTGCCCCCTGGGCCTGAGGCCCCCTGTGTCCAAGCCTCCCCCTGGCTCTTCAGTTCTCTAG CCCTTGGCTCTGCTGGGTTTCCTGACTGTAGCCACATCTCTCCCGCTCCCTAAGGGTAACCTAGCCAATGGAAGCTGCCCTTTGGGTAGGTGCTGGGCTC CTGGGAGGGCCCAGATGATGGGGTGAGGCATGTCTTTCCAGAACTTTCCCTGGCAGGGAGGGGATGGCAGAAACTCAGGGAGGGGCTTGGGGCCCATTGT ATCTGGAGAGCCTGGATTCCTCTTGGCAGTCTTAGGCCCGGCCACTTCTGCTACCTTTGCGCTGCTGTGAGCCTCACCCTGGGCCCCTGGGCCCTGCTTC TCTGCTCCCCTGGGTGATGGGTGGGCCCAGAAGGTGGCAGTCCCACACCTTGTCCTCCCACCTCCCTGAACTGTCCATTGCTTTTATAGGGTGAGGTAAG AGACAGCCTCCCAAGCCCAGGCTTTGGCACTCAGAATGGGCCCAGTGGGGGCTGGGCAGGCCCATTGAGGGCCACCGCCGAGGTTTCTCCTAGGGCTGTT CCTGGGCCTGGCTCTTACAGGCTCGTCCCCCAGGCCTGCCCTTCTCCACTGCCCCCTCCTGTGTCTGGGTCCACACACCCTTCAGGAAGGGGGAGCACTG AGAAGCACAGCACAGGGGCTCAGCCTGGGATCCGGTGATGGTCTGGGCAGAGGCTGGGTCAGGAGTCCCAAAGGTCAGTGACAGTTTCTCAGAAGAGGCC CAGCGTCCACCTCTCTCCCAGGGCCAGACAGCCCTTCCTGGCTCCCCCATCCCCCTATGGGCTCCCAGCCCCTTGCACCCTCATTGCTGTTCAGATTAAA GCCTCTGTTTTGCACCTGT

refseq_PPP2R4.F1 (forward primer)
refseq_PPP2R4.R1 (reverse primer)
Single exon skipping

Frame 0

PH*HGRRPVPRVRRAPAVNRLAP*APRTDMAAV FAVVTLTLGFRL*PAGAHLSSGSSEPLVEPGRGR VGARVKGERGLQASGSAPGRSKMAEGERQPPPD SSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQA YADYIGFILTLNEGVKGKKLTFEYRVSEAIEKLV ALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAK LDEEAENLVATVVPTHLAAAVPEVAVYLKESVG NSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQI AIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWG LDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAV NENHKDYMFLECILFITEMKTGPFAEHSNQLWNI SAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKF GSLLPIHPVTSG*EGPSRRATQATVPVPAFPTP AVAPPHPLPLFVPFDERLFTGVGWRDGLEGAQSI RLQGPSWEK*PKCSQFSEFPCARLARREGLGPG HSATLSCFWPLLPSLPSSLVLRAGAVLQHRREG RRDTCCFHCFSLPGVDAFLRVGAAPCRGGSVSQA MPGWPSMVGLEAEAGRQLWAGVGWVGSGGGEAL AVLAGAPPRLLFTLPPGPEAPCVQASPWLFSSL ALGSAGFPDCSHISPAP*G*PSQWKLPFG*VLGS WEGPDDGVRHVFPELSLAGRGWQKLREGLGAHC IWRAWIPLGSLRPGHFCYLCAAVSLTLGPWALL LCSPG*WVGPEGGSPTPCPPTSLNCPLLL*GEVR DSLPSPGFGTQNGPSGGWAGPLRATAEVSPRAV PGPGSYRLVPQACPSPLPPPVSGSTHPSGRGST EKHSTGAQPGIR*WSGQRLGQESQRSVTVSQKRP SVHLSPRARQPFLAPPSPYGLPAPCTLIAVQIK ASVLHL

Frame 1

RTDMAVARFRASAARRPLIGLLPERPAPTWRPS SLW*L*LSVFGYSRPALTCLQEARSLWWSRGEE GWVQE*KARGDCKHPGRLLAGARWLRASGSRRQI LQRRPLQPLRTSSFQKRRSTQFQTWANGSVLRH TLTTSDSSLPSTKV*RGRS*PSSTESPRPLRN* SLFSTRWTGGLMRLLQWTSPLGLGIRHTGPGMPN LMRKQKTWWPQWSLPIWQLLCLRWLFT*RSQWG TPRALTTAQGMRQPSLLSSAVSARLGCSGWMTK *LLSSRCSIGTLRLCGNSRKHTGWSQPAARECGV WMTSSFCPSSGAVRS**TTHTWSPDTLWMRRP* MRTTRTTCSWSVSCLLPR*RLAHLQSTPTSCGT SAPSLPGPK*TRVSSACIRPSAWRSSL*SSTSSS GACCPSILSRRARRGQAEEPPRPQFLCLPSPPQ QWPLPIPSLCSSRLMRGCLLGWGGEMGLRGLRA *GFRAQVGRSDQSVASFLSSRVLDWPEERVWGLV TRPLSPVSGLFSLHSRPVWF*EQGLFCSTAGKG GEIPAASIAFPFLESMPF*GLELLLAGAGQFPR PCRGGHLW*GWKLRLAASCGLGWGGWGRVVERP* LSWLVPLPGSFSPCPLGLRPPVSKPPPGSSVL* PLALLGFLTVATSLPLPKGNLANGSCPLGRCWA PGRAQMMG*GMSFQNFPWQGGDGRNSGRGLGPIV SGEPGFLLAVLGPATSATFALL*ASPWAPGPCF SAPLGDGWAQKVAVPHLVLPPP*TVHCFYRVR* ETASQAQALALRMGPVGAGQAH*GPPPRFLLGLF LGLALTGSSPRPALLHCPLLCLGPHTLQEGGAL RSTAQGLSLGSGDGLGRGWVRSPKGQ*QFLRRG PASTSLPGPDSPSWLPHPPMGSQPLAPSLLFRLK PLFCTC

Frame 2

ALTWPSPGSARPPRAGR**ACSLSAPHRHGGRL RCGDFNSRFSVIAGRRSLVFRKLGAFGGAGERK GGCKSERREGTASIRVGSWPEQDG*GRAAAAAR FFRGGPSSHSELHHSKKGDPHSSRHGQMEAFSGI R*LHRIHPYPQRRCEGEEADLRVQSLRGH*ETS RSSQHAGQVD**DSSSGPALSVWE*GIQDLVCQ T**GSRKLGGHSGPYPSGSCCA*GGCLPKGVSGE LHAH*LRHRA*GSLRCFPLLSLQDWGAPGG*PN SYCLQGVQSVP*GYAETPENIQDGASRQPGSVG SG*LPVSALHLGQFAADRPPIPGAQTLCG*EGRE *EPQGLHVPGVYPVYYRDEDWPICRALQPAVEH QRRPFLVQSEPGSHPHV*GRVPGEVPCDPALQV REPAAHPSCHVGLGGAKPKSHPGHSSCACLPHPS SGPSPSPPSVRPV**EAVYWGGVARWA*GGSEH KASGPKLGEVTKV*PVF*VPVC*TGQKRGSGAW SLGHSLLFLASSPFTPVQSGFESRGCSAAPQGRE ERYLLLPLLFPSWSRCLSKGWSCSLQGRVSFPG HAGVAIYGRAGS*GWPPAVGWGGVGGVGWWRGL SCPGWCPSQAPFHPAPWA*GPLCPSLPLALQFSS PWLCWVS*L*PHLSRSLRVT*PMEAALWVGAGL LGGPR*WGEACLSRTFPGREGMAETQGGAWGPL YLESLDSSWQS*ARPLLLPLRCCEPHPGPLGPAS LLPWVMGGPRRWQSHTLSSHLPELSIAFIG*GK RQPPKPRLWHSEWAQWGLGRPIEGHRRGFS*GC SWAWLLQARPPGLPFSTAPSCVWVHTPFRKGEH* EAQHRGSAWDPVMVWAEAGSGVPKVSDSFSEEA QRPPLSQGQTALPGSPIPLWAPSPLHPHCCSD* SLCFAP

Sequence NM_178001

CCGCACTGACATGGCCGTCGCCCGGTTCCGCGCGTCCGCCGCGCGCCGGCCGTTAATAGGCTTGCTCCCTGAGCGCCCCGCACCGACATGGCGGCCGTCT TCGCTGTGGTGACTTTAACTCTCGGTTTTCGGTTATAGCCGGCCGGCGCTCACTTGTCTTCAGGAAGCTCGGAGCCTTTGGTGGAGCCGGGGAGAGGAAG GGTGGGTGCAAGAGTGAAAGGCGAGAGGGGACTGCAAGCATCCGGGTCGGCTCCTGGCCGGAGCAAGATGGCTGAGGGCGAGCGGCAGCCGCCGCCAGAT TCTTCAGAGGAGGCCCCTCCAGCCACTCAGAACTTCATCATTCCAAAAAAGGAGATCCACACAGTTCCAGACATGGGCAAATGGAAGCGTTCTCAGGCAT ACGCTGACTACATCGGATTCATCCTTACCCTCAACGAAGGTGTGAAGGGGAAGAAGCTGACCTTCGAGTACAGAGTCTCCGAGATGTGGAATGAGGTTCA TGAGGAAAAGGAGCAGGCTGCAAAGCAGAGTGTGTCCTGCGATGAATGCATACCATTACCCCGCGCCGGGCACTGTGCACCTTCGGAGGCCATTGAGAAA CTAGTCGCTCTTCTCAACACGCTGGACAGGTGGATTGATGAGACTCCTCCAGTGGACCAGCCCTCTCGGTTTGGGAATAAGGCATACAGGACCTGGTATG CCAAACTTGATGAGGAAGCAGAAAACTTGGTGGCCACAGTGGTCCCTACCCATCTGGCAGCTGCTGTGCCTGAGGTGGCTGTTTACCTAAAGGAGTCAGT GGGGAACTCCACGCGCATTGACTACGGCACAGGGCATGAGGCAGCCTTCGCTGCTTTCCTCTGCTGTCTCTGCAAGATTGGGGTGCTCCGGGTGGATGAC CAAATAGCTATTGTCTTCAAGGTGTTCAATCGGTACCTTGAGGTTATGCGGAAACTCCAGAAAACATACAGGATGGAGCCAGCCGGCAGCCAGGGAGTGT GGGGTCTGGATGACTTCCAGTTTCTGCCCTTCATCTGGGGCAGTTCGCAGCTGATAGACCACCCATACCTGGAGCCCAGACACTTTGTGGATGAGAAGGC CGTGAATGAGAACCACAAGGACTACATGTTCCTGGAGTGTATCCTGTTTATTACCGAGATGAAGACTGGCCCATTTGCAGAGCACTCCAACCAGCTGTGG AACATCAGCGCCGTCCCTTCCTGGTCCAAAGTGAACCAGGGTCTCATCCGCATGTATAAGGCCGAGTGCCTGGAGAAGTTCCCTGTGATCCAGCACTTCA AGTTCGGGAGCCTGCTGCCCATCCATCCTGTCACGTCGGGCTAGGAGGGGCCAAGCCGAAGAGCCACCCAGGCCACAGTTCCTGTGCCTGCCTTCCCCAC CCCAGCAGTGGCCCCTCCCCATCCCCTCCCTCTGTTCGTCCCGTTTGATGAGAGGCTGTTTACTGGGGTGGGGTGGCGAGATGGGCTTGAGGGGGCTCAG AGCATAAGGCTTCAGGGCCCAAGTTGGGAGAAGTGACCAAAGTGTAGCCAGTTTTCTGAGTTCCCGTGTGCTAGACTGGCCAGAAGAGAGGGTCTGGGGC CTGGTCACTCGGCCACTCTCTCCTGTTTCTGGCCTCTTCTCCCTTCACTCCCGTCCAGTCTGGTTTTGAGAGCAGGGGCTGTTCTGCAGCACCGCAGGGA AGGGAGGAGAGATACCTGCTGCTTCCATTGCTTTTCCCTTCCTGGAGTCGATGCCTTTCTAAGGGTTGGAGCTGCTCCTTGCAGGGGCGGGTCAGTTTCC CAGGCCATGCCGGGGTGGCCATCTATGGTAGGGCTGGAAGCTGAGGCTGGCCGCCAGCTGTGGGCTGGGGTGGGGTGGGTGGGGTCGGGTGGTGGAGAGG CCTTAGCTGTCCTGGCTGGTGCCCCTCCCAGGCTCCTTTTCACCCTGCCCCCTGGGCCTGAGGCCCCCTGTGTCCAAGCCTCCCCCTGGCTCTTCAGTTC TCTAGCCCTTGGCTCTGCTGGGTTTCCTGACTGTAGCCACATCTCTCCCGCTCCCTAAGGGTAACCTAGCCAATGGAAGCTGCCCTTTGGGTAGGTGCTG GGCTCCTGGGAGGGCCCAGATGATGGGGTGAGGCATGTCTTTCCAGAACTTTCCCTGGCAGGGAGGGGATGGCAGAAACTCAGGGAGGGGCTTGGGGCCC ATTGTATCTGGAGAGCCTGGATTCCTCTTGGCAGTCTTAGGCCCGGCCACTTCTGCTACCTTTGCGCTGCTGTGAGCCTCACCCTGGGCCCCTGGGCCCT GCTTCTCTGCTCCCCTGGGTGATGGGTGGGCCCAGAAGGTGGCAGTCCCACACCTTGTCCTCCCACCTCCCTGAACTGTCCATTGCTTTTATAGGGTGAG GTAAGAGACAGCCTCCCAAGCCCAGGCTTTGGCACTCAGAATGGGCCCAGTGGGGGCTGGGCAGGCCCATTGAGGGCCACCGCCGAGGTTTCTCCTAGGG CTGTTCCTGGGCCTGGCTCTTACAGGCTCGTCCCCCAGGCCTGCCCTTCTCCACTGCCCCCTCCTGTGTCTGGGTCCACACACCCTTCAGGAAGGGGGAG CACTGAGAAGCACAGCACAGGGGCTCAGCCTGGGATCCGGTGATGGTCTGGGCAGAGGCTGGGTCAGGAGTCCCAAAGGTCAGTGACAGTTTCTCAGAAG AGGCCCAGCGTCCACCTCTCTCCCAGGGCCAGACAGCCCTTCCTGGCTCCCCCATCCCCCTATGGGCTCCCAGCCCCTTGCACCCTCATTGCTGTTCAGA TTAAAGCCTCTGTTTTGCACCTGT

refseq_PPP2R4.F1 (forward primer)
refseq_PPP2R4.R1 (reverse primer)
Single exon skipping

Frame 0

PH*HGRRPVPRVRRAPAVNRLAP*APRTDMAAV FAVVTLTLGFRL*PAGAHLSSGSSEPLVEPGRGR VGARVKGERGLQASGSAPGRSKMAEGERQPPPD SSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQA YADYIGFILTLNEGVKGKKLTFEYRVSEMWNEVH EEKEQAAKQSVSCDECIPLPRAGHCAPSEAIEK LVALLNTLDRWIDETPPVDQPSRFGNKAYRTWY AKLDEEAENLVATVVPTHLAAAVPEVAVYLKESV GNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDD QIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGV WGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKA VNENHKDYMFLECILFITEMKTGPFAEHSNQLW NISAVPSWSKVNQGLIRMYKAECLEKFPVIQHF KFGSLLPIHPVTSG*EGPSRRATQATVPVPAFPT PAVAPPHPLPLFVPFDERLFTGVGWRDGLEGAQ SIRLQGPSWEK*PKCSQFSEFPCARLARREGLG PGHSATLSCFWPLLPSLPSSLVLRAGAVLQHRRE GRRDTCCFHCFSLPGVDAFLRVGAAPCRGGSVS QAMPGWPSMVGLEAEAGRQLWAGVGWVGSGGGE ALAVLAGAPPRLLFTLPPGPEAPCVQASPWLFSS LALGSAGFPDCSHISPAP*G*PSQWKLPFG*VL GSWEGPDDGVRHVFPELSLAGRGWQKLREGLGA HCIWRAWIPLGSLRPGHFCYLCAAVSLTLGPWAL LLCSPG*WVGPEGGSPTPCPPTSLNCPLLL*GE VRDSLPSPGFGTQNGPSGGWAGPLRATAEVSPR AVPGPGSYRLVPQACPSPLPPPVSGSTHPSGRGS TEKHSTGAQPGIR*WSGQRLGQESQRSVTVSQK RPSVHLSPRARQPFLAPPSPYGLPAPCTLIAVQ IKASVLHL

Frame 1

RTDMAVARFRASAARRPLIGLLPERPAPTWRPS SLW*L*LSVFGYSRPALTCLQEARSLWWSRGEE GWVQE*KARGDCKHPGRLLAGARWLRASGSRRQI LQRRPLQPLRTSSFQKRRSTQFQTWANGSVLRH TLTTSDSSLPSTKV*RGRS*PSSTESPRCGMRF MRKRSRLQSRVCPAMNAYHYPAPGTVHLRRPLRN *SLFSTRWTGGLMRLLQWTSPLGLGIRHTGPGM PNLMRKQKTWWPQWSLPIWQLLCLRWLFT*RSQ WGTPRALTTAQGMRQPSLLSSAVSARLGCSGWMT K*LLSSRCSIGTLRLCGNSRKHTGWSQPAAREC GVWMTSSFCPSSGAVRS**TTHTWSPDTLWMRR P*MRTTRTTCSWSVSCLLPR*RLAHLQSTPTSCG TSAPSLPGPK*TRVSSACIRPSAWRSSL*SSTS SSGACCPSILSRRARRGQAEEPPRPQFLCLPSP PQQWPLPIPSLCSSRLMRGCLLGWGGEMGLRGLR A*GFRAQVGRSDQSVASFLSSRVLDWPEERVWG LVTRPLSPVSGLFSLHSRPVWF*EQGLFCSTAG KGGEIPAASIAFPFLESMPF*GLELLLAGAGQFP RPCRGGHLW*GWKLRLAASCGLGWGGWGRVVER P*LSWLVPLPGSFSPCPLGLRPPVSKPPPGSSV L*PLALLGFLTVATSLPLPKGNLANGSCPLGRCW APGRAQMMG*GMSFQNFPWQGGDGRNSGRGLGP IVSGEPGFLLAVLGPATSATFALL*ASPWAPGP CFSAPLGDGWAQKVAVPHLVLPPP*TVHCFYRVR *ETASQAQALALRMGPVGAGQAH*GPPPRFLLG LFLGLALTGSSPRPALLHCPLLCLGPHTLQEGG ALRSTAQGLSLGSGDGLGRGWVRSPKGQ*QFLRR GPASTSLPGPDSPSWLPHPPMGSQPLAPSLLFR LKPLFCTC

Frame 2

ALTWPSPGSARPPRAGR**ACSLSAPHRHGGRL RCGDFNSRFSVIAGRRSLVFRKLGAFGGAGERK GGCKSERREGTASIRVGSWPEQDG*GRAAAAAR FFRGGPSSHSELHHSKKGDPHSSRHGQMEAFSGI R*LHRIHPYPQRRCEGEEADLRVQSLRDVE*GS *GKGAGCKAECVLR*MHTITPRRALCTFGGH*E TSRSSQHAGQVD**DSSSGPALSVWE*GIQDLVC QT**GSRKLGGHSGPYPSGSCCA*GGCLPKGVS GELHAH*LRHRA*GSLRCFPLLSLQDWGAPGG* PNSYCLQGVQSVP*GYAETPENIQDGASRQPGSV GSG*LPVSALHLGQFAADRPPIPGAQTLCG*EG RE*EPQGLHVPGVYPVYYRDEDWPICRALQPAV EHQRRPFLVQSEPGSHPHV*GRVPGEVPCDPALQ VREPAAHPSCHVGLGGAKPKSHPGHSSCACLPH PSSGPSPSPPSVRPV**EAVYWGGVARWA*GGS EHKASGPKLGEVTKV*PVF*VPVC*TGQKRGSGA WSLGHSLLFLASSPFTPVQSGFESRGCSAAPQG REERYLLLPLLFPSWSRCLSKGWSCSLQGRVSF PGHAGVAIYGRAGS*GWPPAVGWGGVGGVGWWRG LSCPGWCPSQAPFHPAPWA*GPLCPSLPLALQF SSPWLCWVS*L*PHLSRSLRVT*PMEAALWVGA GLLGGPR*WGEACLSRTFPGREGMAETQGGAWGP LYLESLDSSWQS*ARPLLLPLRCCEPHPGPLGP ASLLPWVMGGPRRWQSHTLSSHLPELSIAFIG* GKRQPPKPRLWHSEWAQWGLGRPIEGHRRGFS*G CSWAWLLQARPPGLPFSTAPSCVWVHTPFRKGE H*EAQHRGSAWDPVMVWAEAGSGVPKVSDSFSE EAQRPPLSQGQTALPGSPIPLWAPSPLHPHCCSD *SLCFAP