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Sequences

Sequence NM_002713

CTTCCAGTTTCCCGGCGTGCTTAGGGCGCGCCAAATGGGAGGGGGAGACGCAAGATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACT GCCCAACCTGGTGAGTGGCGGGGCGGCCAGGGCTAGAGTGGCCCGGCCGGAGCTAGCCTGGGCTGGAAGGGCGGCTCTTTTTTTACTTTTCTGCTGCGAG CCGAACGGCTCAGAAACCCCGGAATGGTTGAGGAAAAACTGTTTGCTGCACCGGGCCGGGCGACGTGTTGAAGAACCGAGAGCCTGGAGCCCAGGCCCAG GAACTGAAGAAACCCGGGGTTGGGGGCTCAAAGGCGCTCACTTAGGCAGCCCCTTTGAGCGATTAGCCAGTCGCCGGAGCGCTTCGAGGCCTTGGCCCGA ACTTACGCCCAACTCTTGACTGAGTGCCTGGTGCTCTCGTGGAGCATCGCATCTGGCCCCTTCCTGTACGTCCCGAGCGCGCTCGAGCCAGCCCCGGCCC CAACCCTACCTCCAAGCCCCGCATCCCTCTGTGGTTGCTGCATCCCTCGTGCGGCACTTGTCTGTCTGCCACAGAGAATACGAGGGGCAGGTAAGCCCCC TCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATTATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTG TGTGACTTTACCATTGACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTGAAGAGAGTTTTCCTGATAGATCTCAACAGTA AACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTTACCATTGAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAGAGGAAGA ACTCACGGGTGACATTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTGGATCCCTCAGTTGGTCGATTCAGGAACATGGTGCAAACTGCAGTGGT CCCAGTCAAGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGCGGAGGACTCTAC GGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGCATCCATGGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTG CCCCTGATGTGGACTTGACTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTATAACCCTGAAGCTGTAAATGAACCCAA GAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAGCCCACACCTTCCTTGCTGATTTGATATTTTTGGTCATGGAGAAGGGTGGGATTGGGTGGG AATGGGGTGGAAGGGTGATGGGGAGCTAATGAACTAGGGAGAAAAACTTTCCATGTGTGCGGTATCGTCTTTCAGAATGTCTCCTGGCATCCTAACCATG TAATATGACAATTGGGGGTGGGGTTGAAATAGCCCATAAAGACCTGTCTTCACAACACTTGCATTGTAGAGAAAGGCTTCTTATATCCTTTTCAATAGAC TGCCCTGGCTCTTTCCTAGGCCTTCCACTACCTCCTTTCTTTCTCCCACTTTCTAGGATCATTTTTATGTAAAGTCACATATCCCAGGCCCTCAGGTTGA ATCCAGAGCTGTAGAGGTTACAGTAGCATCACCAGCCTTGGGGGTCCAGAGCCTAATTTATATTCACTATCCTTCCAAGTCCCGGGTAGCAGAAGGGTTG CCATAGATCTCAGTTTGATCAAAAAGAAGGCTTAGAATTCTGCAGTTAAGCTGAGGTTTAAACTAAAAAATGTTTCCTTGGGTCAGTGGTTTTGAGGTCC AGTAGCTAGGCTTTTCTCTTTTGTCCTTCCTGTTGGAATGAAAACATTTCGATTTTCCTTCATCTGTGACTGGTGCCATAGACACAGGTTTATAGTTTTA ACTTACAGTATTGTTTGAAATTTACCTGTTTTTCTTGTCAAACCTGAGCACTCCTCCTGCTGAAGTTTCTTATTTAATTCCAGAGTACTGTCCTCTACTC TAAGGCATTACTTTTAAGTGTATTATGAAGGCAGTTTTCAAAGGATATGACCAGTTGGGGTAATTCAAATTAAAAAGGAAAAGATTTGTTTGGAAGTAAC TGGTGTCTCTAAGAGGAATTTTTAGATGTCAGTTTGGAGGCTCTTTCCCCCCTCAATTGAGAGCTCTTGTTATTCAGAGCTCCAAGACTAGACCTGGCTA ACAAACATAGGAGACAAAGTTAGGAAACATTGATACAAGCTTTGTACAGAGATTTGTACATTTGTGTAATAGGCCTTTTCATGCTTTATGTGTAGCTTTT TACCTGTAACCTTTATTACATTGTAAATTAAACGTAACTTTTGTCATTTGGGTGCAGGCTGTGAATTTGTCTCTCAGTCACTGATTGCCACTGCCATCTG GAAATGTTTGCTAAAGGCACAGTCACTGGGCTTGGGAGGCAATGCTCCATCCCCATTATATTACAAATAAAGATGCCCTAAATGAGTGTG

refseq_PPP1R8.F3 (forward primer)
refseq_PPP1R8.R3 (reverse primer)
Single exon skipping

Frame 0

LPVSRRA*GAPNGRGRRKMAAAANSGSSLPLFD CPTW*VAGRPGLEWPGRS*PGLEGRLFFYFSAAS RTAQKPRNG*GKTVCCTGPGDVLKNREPGAQAQ ELKKPGVGGSKALT*AAPLSD*PVAGALRGLGP NLRPTLD*VPGALVEHRIWPLPVRPERARASPGP NPTSKPRIPLWLLHPSCGTCLSATENTRGR*AP SRFTSGCSQRRQTN*ETDY**EEVLLIWEKP*F V*LYH*PPVLLSGPCCTCLPQASEESFPDRSQQ* T*QSSTLPTTSGFLPLPLRREIWTFKDQRGRGR THG*HSVRMMRSSTQRMWIPQLVDSGTWCKLQW SQSRRSVWRALAPWAWRNQGAGACRTLPSAEDST GACPPHTVKQAPSHMASMGQHSSVACPCHTQTL PLMWT*LLLCRQQ*T*TLHQTLQSITLKL*MNP RRRNMQKRLGQARSPHLPC*FDIFGHGEGWDWVG MGWKGDGELMN*GEKLSMCAVSSFRMSPGILTM *YDNWGWG*NSP*RPVFTTLAL*RKASYILFNR LPWLFPRPSTTSFLSPTF*DHFYVKSHIPGPQVE SRAVEVTVASPALGVQSLIYIHYPSKSRVAEGL P*ISV*SKRRLRILQLS*GLN*KMFPWVSGFEV Q*LGFSLLSFLLE*KHFDFPSSVTGAIDTGL*F* LTVLFEIYLFFLSNLSTPPAEVSYLIPEYCPLL *GITFKCIMKAVFKGYDQLG*FKLKRKRFVWK* LVSLRGIFRCQFGGSFPPQLRALVIQSSKTRPG* QT*ETKLGNIDTSFVQRFVHLCNRPFHALCVAF YL*PLLHCKLNVTFVIWVQAVNLSLSH*LPLPS GNVC*RHSHWAWEAMLHPHYITNKDALNEC

Frame 1

FQFPGVLRARQMGGGDARWRQPRTPALASRCST AQPGEWRGGQG*SGPAGASLGWKGGSFFTFLLR AERLRNPGMVEEKLFAAPGRATC*RTESLEPRPR N*RNPGLGAQRRSLRQPL*AISQSPERFEALAR TYAQLLTECLVLSWSIASGPFLYVPSALEPAPA PTLPPSPASLCGCCIPRAALVCLPQRIRGAGKPP PGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDL CDFTIDHQSCSRVHAALVYHKHLKRVFLIDLNS KPDRVQHCPQQADFYPYH*GGKSGHSKTKEEEEE LTGDIQ*G**DHQPRGCGSLSWSIQEHGANCSG PSQEEACGGPWLPGPGGIREQAHAELCLQRRTL RGPAPHTQ*SRLPATWHPWDSTHRWLAHAIPKPC P*CGLDSCCAVSSEHEPCTKPCSL*P*SCK*TQ EEEICKRGLARQEAHTFLADLIFLVMEKGGIGW EWGGRVMGS**TREKNFPCVRYRLSECLLAS*PC NMTIGGGVEIAHKDLSSQHLHCRERLLISFSID CPGSFLGLPLPPFFLPLSRIIFM*SHISQALRL NPEL*RLQ*HHQPWGSRA*FIFTILPSPG*QKGC HRSQFDQKEGLEFCS*AEV*TKKCFLGSVVLRS SS*AFLFCPSCWNENISIFLHL*LVP*TQVYSF NLQYCLKFTCFSCQT*ALLLLKFLI*FQSTVLYS KALLLSVL*RQFSKDMTSWGNSN*KGKDLFGSN WCL*EEFLDVSLEALSPLN*ELLLFRAPRLDLA NKHRRQS*ETLIQALYRDLYICVIGLFMLYV*LF TCNLYYIVN*T*LLSFGCRL*ICLSVTDCHCHL EMFAKGTVTGLGRQCSIPIILQIKMP*MSV

Frame 2

SSFPACLGRAKWEGETQDGGSRELRL*PPAVRL PNLVSGGAARARVARPELAWAGRAALFLLFCCE PNGSETPEWLRKNCLLHRAGRRVEEPRAWSPGP GTEETRGWGLKGAHLGSPFERLASRRSASRPWPE LTPNS*LSAWCSRGASHLAPSCTSRARSSQPRP QPYLQAPHPSVVAASLVRHLSVCHREYEGQVSP LPVYIWM*SKETN*LRN*LLMRRSITYLGETLIC VTLPLTTSLALGSMLHLSTTSI*REFS**ISTV NLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRK NSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVV PVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLY GGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNL APDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPK KKKYAKEAWPGKKPTPSLLI*YFWSWRRVGLGG NGVEG*WGANELGRKTFHVCGIVFQNVSWHPNH VI*QLGVGLK*PIKTCLHNTCIVEKGFLYPFQ*T ALALS*AFHYLLSFSHFLGSFLCKVTYPRPSG* IQSCRGYSSITSLGGPEPNLYSLSFQVPGSRRV AIDLSLIKKKA*NSAVKLRFKLKNVSLGQWF*GP VARLFSFVLPVGMKTFRFSFICDWCHRHRFIVL TYSIV*NLPVFLVKPEHSSC*SFLFNSRVLSST LRHYF*VYYEGSFQRI*PVGVIQIKKEKICLEVT GVSKRNF*MSVWRLFPPSIESSCYSELQD*TWL TNIGDKVRKH*YKLCTEICTFV**AFSCFMCSF LPVTFITL*IKRNFCHLGAGCEFVSQSLIATAIW KCLLKAQSLGLGGNAPSPLYYK*RCPK*V

Sequence NM_014110

CTTCCAGTTTCCCGGCGTGCTTAGGGCGCGCCAAATGGGAGGGGGAGACGCAAGATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACT GCCCAACCTGGGCAGGTAAGCCCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAACTGATTATTGATGAGAAGAAGTATTA CTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATTGACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTGAAGAGA GTTTTCCTGATAGATCTCAACAGTACACACGGCACTTTCTTGGGTCACATTCGGTTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCT CATTTGGCGCATCCACAAGGGCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGATGAGAAGATGGGTGGAGAGGATGATGA ACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAAACTGAGCTTGATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTTACCATT GAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAGAGGAAGAACTCACGGGTGACATTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTGG ATCCCTCAGTTGGTCGATTCAGGAACATGGTGCAAACTGCAGTGGTCCCAGTCAAGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTGGGCCTGGAGGAATC AGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGCGGAGGACTCTACGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGCATCCAT GGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGATGTGGACTTGACTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTG CACCAAACCCTGCAGTCTATAACCCTGAAGCTGTAAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAGCCCACACCTTCCTT GCTGATTTGATATTTTTGGTCATGGAGAAGGGTGGGATTGGGTGGGAATGGGGTGGAAGGGTGATGGGGAGCTAATGAACTAGGGAGAAAAACTTTCCAT GTGTGCGGTATCGTCTTTCAGAATGTCTCCTGGCATCCTAACCATGTAATATGACAATTGGGGGTGGGGTTGAAATAGCCCATAAAGACCTGTCTTCACA ACACTTGCATTGTAGAGAAAGGCTTCTTATATCCTTTTCAATAGACTGCCCTGGCTCTTTCCTAGGCCTTCCACTACCTCCTTTCTTTCTCCCACTTTCT AGGATCATTTTTATGTAAAGTCACATATCCCAGGCCCTCAGGTTGAATCCAGAGCTGTAGAGGTTACAGTAGCATCACCAGCCTTGGGGGTCCAGAGCCT AATTTATATTCACTATCCTTCCAAGTCCCGGGTAGCAGAAGGGTTGCCATAGATCTCAGTTTGATCAAAAAGAAGGCTTAGAATTCTGCAGTTAAGCTGA GGTTTAAACTAAAAAATGTTTCCTTGGGTCAGTGGTTTTGAGGTCCAGTAGCTAGGCTTTTCTCTTTTGTCCTTCCTGTTGGAATGAAAACATTTCGATT TTCCTTCATCTGTGACTGGTGCCATAGACACAGGTTTATAGTTTTAACTTACAGTATTGTTTGAAATTTACCTGTTTTTCTTGTCAAACCTGAGCACTCC TCCTGCTGAAGTTTCTTATTTAATTCCAGAGTACTGTCCTCTACTCTAAGGCATTACTTTTAAGTGTATTATGAAGGCAGTTTTCAAAGGATATGACCAG TTGGGGTAATTCAAATTAAAAAGGAAAAGATTTGTTTGGAAGTAACTGGTGTCTCTAAGAGGAATTTTTAGATGTCAGTTTGGAGGCTCTTTCCCCCCTC AATTGAGAGCTCTTGTTATTCAGAGCTCCAAGACTAGACCTGGCTAACAAACATAGGAGACAAAGTTAGGAAACATTGATACAAGCTTTGTACAGAGATT TGTACATTTGTGTAATAGGCCTTTTCATGCTTTATGTGTAGCTTTTTACCTGTAACCTTTATTACATTGTAAATTAAACGTAACTTTTGTCATTTGGGTG CAGGCTGTGAATTTGTCTCTCAGTCACTGATTGCCACTGCCATCTGGAAATGTTTGCTAAAGGCACAGTCACTGGGCTTGGGAGGCAATGCTCCATCCCC ATTATATTACAAATAAAGATGCCCTAAATGAGTGTG

refseq_PPP1R8.F3 (forward primer)
refseq_PPP1R8.R3 (reverse primer)
Single exon skipping

Frame 0

LPVSRRA*GAPNGRGRRKMAAAANSGSSLPLFD CPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYY LFGRNPDLCDFTIDHQSCSRVHAALVYHKHLKR VFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTV SFGASTRAYTLREKPQTLPSAVKGDEKMGGEDDE LKGLLGLPEEETELDNLTEFNTAHNKRISTLTI EEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDV DPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEES GSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIH GTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNP APNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSL LI*YFWSWRRVGLGGNGVEG*WGANELGRKTFH VCGIVFQNVSWHPNHVI*QLGVGLK*PIKTCLH NTCIVEKGFLYPFQ*TALALS*AFHYLLSFSHFL GSFLCKVTYPRPSG*IQSCRGYSSITSLGGPEP NLYSLSFQVPGSRRVAIDLSLIKKKA*NSAVKL RFKLKNVSLGQWF*GPVARLFSFVLPVGMKTFRF SFICDWCHRHRFIVLTYSIV*NLPVFLVKPEHS SC*SFLFNSRVLSSTLRHYF*VYYEGSFQRI*P VGVIQIKKEKICLEVTGVSKRNF*MSVWRLFPPS IESSCYSELQD*TWLTNIGDKVRKH*YKLCTEI CTFV**AFSCFMCSFLPVTFITL*IKRNFCHLG AGCEFVSQSLIATAIWKCLLKAQSLGLGGNAPSP LYYK*RCPK*V

Frame 1

FQFPGVLRARQMGGGDARWRQPRTPALASRCST AQPGQVSPLPVYIWM*SKETN*LRN*LLMRRSI TYLGETLICVTLPLTTSLALGSMLHLSTTSI*RE FS**ISTVHTALSWVTFGWNLTSLSKFPSIPRS HLAHPQGHTLCARSLRHCHRL*KEMRRWVERMM NSRAYWGFQRRKLSLIT*QSSTLPTTSGFLPLPL RREIWTFKDQRGRGRTHG*HSVRMMRSSTQRMW IPQLVDSGTWCKLQWSQSRRSVWRALAPWAWRN QGAGACRTLPSAEDSTGACPPHTVKQAPSHMASM GQHSSVACPCHTQTLPLMWT*LLLCRQQ*T*TL HQTLQSITLKL*MNPRRRNMQKRLGQARSPHLP C*FDIFGHGEGWDWVGMGWKGDGELMN*GEKLSM CAVSSFRMSPGILTM*YDNWGWG*NSP*RPVFT TLAL*RKASYILFNRLPWLFPRPSTTSFLSPTF *DHFYVKSHIPGPQVESRAVEVTVASPALGVQSL IYIHYPSKSRVAEGLP*ISV*SKRRLRILQLS* GLN*KMFPWVSGFEVQ*LGFSLLSFLLE*KHFD FPSSVTGAIDTGL*F*LTVLFEIYLFFLSNLSTP PAEVSYLIPEYCPLL*GITFKCIMKAVFKGYDQ LG*FKLKRKRFVWK*LVSLRGIFRCQFGGSFPP QLRALVIQSSKTRPG*QT*ETKLGNIDTSFVQRF VHLCNRPFHALCVAFYL*PLLHCKLNVTFVIWV QAVNLSLSH*LPLPSGNVC*RHSHWAWEAMLHP HYITNKDALNEC

Frame 2

SSFPACLGRAKWEGETQDGGSRELRL*PPAVRL PNLGR*APSRFTSGCSQRRQTN*ETDY**EEVL LIWEKP*FV*LYH*PPVLLSGPCCTCLPQASEE SFPDRSQQYTRHFLGSHSVGTSQASANSHRFHGL IWRIHKGIHSAREASDIAIGCERR*EDGWRG** TQGLTGASRGGN*A**PDRVQHCPQQADFYPYH *GGKSGHSKTKEEEEELTGDIQ*G**DHQPRGCG SLSWSIQEHGANCSGPSQEEACGGPWLPGPGGI REQAHAELCLQRRTLRGPAPHTQ*SRLPATWHP WDSTHRWLAHAIPKPCP*CGLDSCCAVSSEHEPC TKPCSL*P*SCK*TQEEEICKRGLARQEAHTFL ADLIFLVMEKGGIGWEWGGRVMGS**TREKNFP CVRYRLSECLLAS*PCNMTIGGGVEIAHKDLSSQ HLHCRERLLISFSIDCPGSFLGLPLPPFFLPLS RIIFM*SHISQALRLNPEL*RLQ*HHQPWGSRA *FIFTILPSPG*QKGCHRSQFDQKEGLEFCS*AE V*TKKCFLGSVVLRSSS*AFLFCPSCWNENISI FLHL*LVP*TQVYSFNLQYCLKFTCFSCQT*AL LLLKFLI*FQSTVLYSKALLLSVL*RQFSKDMTS WGNSN*KGKDLFGSNWCL*EEFLDVSLEALSPL N*ELLLFRAPRLDLANKHRRQS*ETLIQALYRD LYICVIGLFMLYV*LFTCNLYYIVN*T*LLSFGC RL*ICLSVTDCHCHLEMFAKGTVTGLGRQCSIP IILQIKMP*MSV

Sequence NM_138558

CTTCCAGTTTCCCGGCGTGCTTAGGGCGCGCCAAATGGGAGGGGGAGACGCAAGATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACT GCCCAACCTGGTGAGTGGCGGGGCGGCCAGGGCTAGAGTGGCCCGGCCGGAGCTAGCCTGGGCTGGAAGGGCGGCTCTTTTTTTACTTTTCTGCTGCGAG CCGAACGGCTCAGAAACCCCGGAATGGTTGAGGAAAAACTGTTTGCTGCACCGGGCCGGGCGACGTGTTGAAGAACCGAGAGCCTGGAGCCCAGGCCCAG GAACTGAAGAAACCCGGGGTTGGGGGCTCAAAGGCGCTCACTTAGGCAGCCCCTTTGAGCGATTAGCCAGTCGCCGGAGCGCTTCGAGGCCTTGGCCCGA ACTTACGCCCAACTCTTGACTGAGTGCCTGGTGCTCTCGTGGAGCATCGCATCTGGCCCCTTCCTGGCAGGTAAGCCCCCTCCCGGTTTACATCTGGATG TAGTCAAAGGAGACAAACTAATTGAGAAACTGATTATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTTACCATTGACCA CCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTGAAGAGAGTTTTCCTGATAGATCTCAACAGTACACACGGCACTTTCTTGGGT CACATTCGGTTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATTTGGCGCATCCACAAGGGCATACACTCTGCGCGAGAAGCCTC AGACATTGCCATCGGCTGTGAAAGGAGATGAGAAGATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAAACTGAGCTTGA TAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTTACCATTGAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAGAGGAAG AACTCACGGGTGACATTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTGGATCCCTCAGTTGGTCGATTCAGGAACATGGTGCAAACTGCAGTGG TCCCAGTCAAGAAGAAGCGTGTGGAGGGCCCTGGCTCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGCGGAGGACTCTA CGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGCATCCATGGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTT GCCCCTGATGTGGACTTGACTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCAGTCTATAACCCTGAAGCTGTAAATGAACCCA AGAAGAAGAAATATGCAAAAGAGGCTTGGCCAGGCAAGAAGCCCACACCTTCCTTGCTGATTTGATATTTTTGGTCATGGAGAAGGGTGGGATTGGGTGG GAATGGGGTGGAAGGGTGATGGGGAGCTAATGAACTAGGGAGAAAAACTTTCCATGTGTGCGGTATCGTCTTTCAGAATGTCTCCTGGCATCCTAACCAT GTAATATGACAATTGGGGGTGGGGTTGAAATAGCCCATAAAGACCTGTCTTCACAACACTTGCATTGTAGAGAAAGGCTTCTTATATCCTTTTCAATAGA CTGCCCTGGCTCTTTCCTAGGCCTTCCACTACCTCCTTTCTTTCTCCCACTTTCTAGGATCATTTTTATGTAAAGTCACATATCCCAGGCCCTCAGGTTG AATCCAGAGCTGTAGAGGTTACAGTAGCATCACCAGCCTTGGGGGTCCAGAGCCTAATTTATATTCACTATCCTTCCAAGTCCCGGGTAGCAGAAGGGTT GCCATAGATCTCAGTTTGATCAAAAAGAAGGCTTAGAATTCTGCAGTTAAGCTGAGGTTTAAACTAAAAAATGTTTCCTTGGGTCAGTGGTTTTGAGGTC CAGTAGCTAGGCTTTTCTCTTTTGTCCTTCCTGTTGGAATGAAAACATTTCGATTTTCCTTCATCTGTGACTGGTGCCATAGACACAGGTTTATAGTTTT AACTTACAGTATTGTTTGAAATTTACCTGTTTTTCTTGTCAAACCTGAGCACTCCTCCTGCTGAAGTTTCTTATTTAATTCCAGAGTACTGTCCTCTACT CTAAGGCATTACTTTTAAGTGTATTATGAAGGCAGTTTTCAAAGGATATGACCAGTTGGGGTAATTCAAATTAAAAAGGAAAAGATTTGTTTGGAAGTAA CTGGTGTCTCTAAGAGGAATTTTTAGATGTCAGTTTGGAGGCTCTTTCCCCCCTCAATTGAGAGCTCTTGTTATTCAGAGCTCCAAGACTAGACCTGGCT AACAAACATAGGAGACAAAGTTAGGAAACATTGATACAAGCTTTGTACAGAGATTTGTACATTTGTGTAATAGGCCTTTTCATGCTTTATGTGTAGCTTT TTACCTGTAACCTTTATTACATTGTAAATTAAACGTAACTTTTGTCATTTGGGTGCAGGCTGTGAATTTGTCTCTCAGTCACTGATTGCCACTGCCATCT GGAAATGTTTGCTAAAGGCACAGTCACTGGGCTTGGGAGGCAATGCTCCATCCCCATTATATTACAAATAAAGATGCCCTAAATGAGTGTG

refseq_PPP1R8.F3 (forward primer)
refseq_PPP1R8.R3 (reverse primer)
Single exon skipping

Frame 0

LPVSRRA*GAPNGRGRRKMAAAANSGSSLPLFD CPTW*VAGRPGLEWPGRS*PGLEGRLFFYFSAAS RTAQKPRNG*GKTVCCTGPGDVLKNREPGAQAQ ELKKPGVGGSKALT*AAPLSD*PVAGALRGLGP NLRPTLD*VPGALVEHRIWPLPGR*APSRFTSGC SQRRQTN*ETDY**EEVLLIWEKP*FV*LYH*P PVLLSGPCCTCLPQASEESFPDRSQQYTRHFLG SHSVGTSQASANSHRFHGLIWRIHKGIHSAREAS DIAIGCERR*EDGWRG**TQGLTGASRGGN*A* *PDRVQHCPQQADFYPYH*GGKSGHSKTKEEEE ELTGDIQ*G**DHQPRGCGSLSWSIQEHGANCSG PSQEEACGGPWLPGPGGIREQAHAELCLQRRTL RGPAPHTQ*SRLPATWHPWDSTHRWLAHAIPKP CP*CGLDSCCAVSSEHEPCTKPCSL*P*SCK*TQ EEEICKRGLARQEAHTFLADLIFLVMEKGGIGW EWGGRVMGS**TREKNFPCVRYRLSECLLAS*P CNMTIGGGVEIAHKDLSSQHLHCRERLLISFSID CPGSFLGLPLPPFFLPLSRIIFM*SHISQALRL NPEL*RLQ*HHQPWGSRA*FIFTILPSPG*QKG CHRSQFDQKEGLEFCS*AEV*TKKCFLGSVVLRS SS*AFLFCPSCWNENISIFLHL*LVP*TQVYSF NLQYCLKFTCFSCQT*ALLLLKFLI*FQSTVLY SKALLLSVL*RQFSKDMTSWGNSN*KGKDLFGSN WCL*EEFLDVSLEALSPLN*ELLLFRAPRLDLA NKHRRQS*ETLIQALYRDLYICVIGLFMLYV*L FTCNLYYIVN*T*LLSFGCRL*ICLSVTDCHCHL EMFAKGTVTGLGRQCSIPIILQIKMP*MSV

Frame 1

FQFPGVLRARQMGGGDARWRQPRTPALASRCST AQPGEWRGGQG*SGPAGASLGWKGGSFFTFLLR AERLRNPGMVEEKLFAAPGRATC*RTESLEPRPR N*RNPGLGAQRRSLRQPL*AISQSPERFEALAR TYAQLLTECLVLSWSIASGPFLAGKPPPGLHLD VVKGDKLIEKLIIDEKKYYLFGRNPDLCDFTIDH QSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLG HIRLEPHKPQQIPIDSTVSFGASTRAYTLREKP QTLPSAVKGDEKMGGEDDELKGLLGLPEEETELD NLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRK NSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAV VPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLY GGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNL APDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEP KKKKYAKEAWPGKKPTPSLLI*YFWSWRRVGLGG NGVEG*WGANELGRKTFHVCGIVFQNVSWHPNH VI*QLGVGLK*PIKTCLHNTCIVEKGFLYPFQ* TALALS*AFHYLLSFSHFLGSFLCKVTYPRPSG* IQSCRGYSSITSLGGPEPNLYSLSFQVPGSRRV AIDLSLIKKKA*NSAVKLRFKLKNVSLGQWF*G PVARLFSFVLPVGMKTFRFSFICDWCHRHRFIVL TYSIV*NLPVFLVKPEHSSC*SFLFNSRVLSST LRHYF*VYYEGSFQRI*PVGVIQIKKEKICLEV TGVSKRNF*MSVWRLFPPSIESSCYSELQD*TWL TNIGDKVRKH*YKLCTEICTFV**AFSCFMCSF LPVTFITL*IKRNFCHLGAGCEFVSQSLIATAI WKCLLKAQSLGLGGNAPSPLYYK*RCPK*V

Frame 2

SSFPACLGRAKWEGETQDGGSRELRL*PPAVRL PNLVSGGAARARVARPELAWAGRAALFLLFCCE PNGSETPEWLRKNCLLHRAGRRVEEPRAWSPGP GTEETRGWGLKGAHLGSPFERLASRRSASRPWPE LTPNS*LSAWCSRGASHLAPSWQVSPLPVYIWM *SKETN*LRN*LLMRRSITYLGETLICVTLPLT TSLALGSMLHLSTTSI*REFS**ISTVHTALSWV TFGWNLTSLSKFPSIPRSHLAHPQGHTLCARSL RHCHRL*KEMRRWVERMMNSRAYWGFQRRKLSL IT*QSSTLPTTSGFLPLPLRREIWTFKDQRGRGR THG*HSVRMMRSSTQRMWIPQLVDSGTWCKLQW SQSRRSVWRALAPWAWRNQGAGACRTLPSAEDS TGACPPHTVKQAPSHMASMGQHSSVACPCHTQTL PLMWT*LLLCRQQ*T*TLHQTLQSITLKL*MNP RRRNMQKRLGQARSPHLPC*FDIFGHGEGWDWV GMGWKGDGELMN*GEKLSMCAVSSFRMSPGILTM *YDNWGWG*NSP*RPVFTTLAL*RKASYILFNR LPWLFPRPSTTSFLSPTF*DHFYVKSHIPGPQV ESRAVEVTVASPALGVQSLIYIHYPSKSRVAEGL P*ISV*SKRRLRILQLS*GLN*KMFPWVSGFEV Q*LGFSLLSFLLE*KHFDFPSSVTGAIDTGL*F *LTVLFEIYLFFLSNLSTPPAEVSYLIPEYCPLL *GITFKCIMKAVFKGYDQLG*FKLKRKRFVWK* LVSLRGIFRCQFGGSFPPQLRALVIQSSKTRPG *QT*ETKLGNIDTSFVQRFVHLCNRPFHALCVAF YL*PLLHCKLNVTFVIWVQAVNLSLSH*LPLPS GNVC*RHSHWAWEAMLHPHYITNKDALNEC