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Sequences

Sequence NM_021003

GTGGTTGGGGAGGGGGGGGTGGGGGGACTCTAGACAGCTGAGGCGCGAAAGCGATGAGTCCTCGGCTCTTCCTCCTCCTTCTCCGGGACCCGCTCTCTGC
CTCCCTCTCCAACGCCCGGATGATCTGAGCCGCGAGGGCGCCGACAGCCGGGGGCCCGGACGCAGCCCGGCTCCTCCCCTCCTCCGCCCCTTCCCCAGCC
TGACCTGGCCCGCCGCTGCAGCGGTGACCCCTCCCCCGGCTGCCGCCGTCGCCGCCGCGGTGACCCCCTCCCCGGCTGCCGCCGCCGCCGCCTCGGCCGA
CCAGGGACCTGCCCGCCTGCGGCTGCTCCGGACCTAGAGGATCAAGACATAATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAATGCCCAGG
GGCAGGGTAATGGGTTGCGATATGGGCTAAGCAGCATGCAAGGCTGGCGTGTTGAAATGGAGGATGCACATACGGCTGTGATCGGTTTGCCAAGTGGACT
TGAATCGTGGTCATTCTTTGCTGTGTATGATGGGCATGCTGGTTCTCAGGTTGCCAAATACTGCTGTGAGCATTTGTTAGATCACATCACCAATAACCAG
GATTTTAAAGGGTCTGCAGGAGCACCTTCTGTGGAAAATGTAAAGAATGGAATCAGAACAGGTTTTCTGGAGATTGATGAACACATGAGAGTTATGTCAG
AGAAGAAACATGGTGCAGATAGAAGTGGGTCAACAGCTGTAGGTGTCTTAATTTCTCCCCAACATACTTATTTCATTAACTGTGGAGACTCAAGAGGTTT
ACTTTGTAGGAACAGGAAAGTTCATTTCTTCACACAAGATCACAAACCAAGTAATCCGCTGGAGAAAGAACGAATTCAGAATGCAGGTGGCTCTGTAATG
ATTCAGCGTGTGAATGGCTCTCTGGCTGTATCGAGGGCCCTTGGGGATTTTGATTACAAATGTGTCCATGGAAAAGGTCCTACTGAGCAGCTTGTCTCAC
CAGAGCCTGAAGTCCATGATATTGAAAGATCTGAAGAAGATGATCAGTTCATTATCCTTGCATGTGATGGTATCTGGGATGTTATGGGAAATGAAGAGCT
CTGTGATTTTGTAAGATCCAGACTTGAAGTCACTGATGACCTTGAGAAAGTTTGCAATGAAGTAGTCGACACCTGTTTGTATAAGGGAAGTCGAGACAAC
ATGAGTGTGATTTTGATCTGTTTTCCAAATGCACCCAAAGTATCGCCAGAAGCAGTGAAGAAGGAGGCAGAGTTGGACAAGTACCTGGAATGCAGAGTAG
AAGAAATCATAAAGAAGCAGGGGGAAGGCGTCCCCGACTTAGTCCATGTGATGCGCACATTAGCGAGTGAGAACATCCCCAGCCTCCCACCAGGGGGTGA
ATTGGCAAGCAAGAGGAATGTTATTGAAGCCGTTTACAATAGACTGAATCCTTACAAAAATGACGACACTGACTCTACATCAACAGATGATATGTGGTAA
AACTGCTCATCTAGCCATGGAGTTTACCTTCACCTCCAAAGGAGAGTACAGCTCAACTTTGTTGAAACTTTTAACATCCATCCTCAACTTTAAGGAAGGG
GATATGACATGGGTGAGAATGATTACATCAGAGAACTTCAGCAGTACAACAGCTAGCCCAGAACTGATTTTTTTTTTTTTTTTTGTAAATTTGAGACTTA
TGTAAGCGTGATTTCAAACCATAATTCGTGTTGTAAATCAGACTCCAGCAATTTTTGTTGTATGATTTTGTTTTTTTGTAAAGTGTAATTGTCCTTGTAC
AAAATGCTCATATTTAATTATGAACTGCTTTAAATCACTATCAAAGTTACAAGAAATGTTTGGCTTATTGTGTGATGCAACAGATATATAGCCCTTTCAA
GTCATGTTGTGTTTGGACTTGGGGTTGGAACAGGGAGAGCAGCAGCCATGTCAGCTACACGCTCAAATGTGCAGATGATTATGGAAAATAACCTCAAAAT
CTTACAAAGCTGAACATCCAAGGAGTTATTGAAAACTATCTTAAATGTTCTTGGTAGGGGAGTTGGCATTGTTGATAAAGCCAGTCCCTTCATTTAACTG
TCTTTCAGGATGTTCCTTCGTTGTTTCCATGAGTATTGCAGGTAATAATACAGTGTATTCATAAGAATCTCAATCTTGGGGCTAAATGCCTTGTTTCTTT
GCACCTCTTTTCAAGTCCTTACATTTAATTACTAATTGATAAGCAGCAGCTTCCTACATATAGTAGGAAACTGCCACATTTTTGCTATCATGATTGGCTG
GGCCTGCTGCTGTTCCTAGTAAGATATTCTGAATTCCATTTTATCAATAAAGCTTGATTTAACAAACAAGAAA
  • refseq_PPM1A.F2 (forward primer)
  • refseq_PPM1A.R2 (reverse primer)
  • Single exon skipping

Frame 0

VVGEGGVGGL*TAEARKR*VLGSSSSFSGTRSL
PPSPTPG*SEPRGRRQPGARTQPGSSPPPPLPQP
DLARRCSGDPSPGCRRRRRGDPLPGCRRRRLGR
PGTCPPAAAPDLEDQDIMGAFLDKPKMEKHNAQ
GQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGL
ESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQ
DFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMS
EKKHGADRSGSTAVGVLISPQHTYFINCGDSRGL
LCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVM
IQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVS
PEPEVHDIERSEEDDQFIILACDGIWDVMGNEEL
CDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDN
MSVILICFPNAPKVSPEAVKKEAELDKYLECRV
EEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGE
LASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW*
NCSSSHGVYLHLQRRVQLNFVETFNIHPQL*GR
GYDMGENDYIRELQQYNS*PRTDFFFFFCKFETY
VSVISNHNSCCKSDSSNFCCMILFFCKV*LSLY
KMLIFNYELL*ITIKVTRNVWLIV*CNRYIALS
SHVVFGLGVGTGRAAAMSATRSNVQMIMENNLKI
LQS*TSKELLKTILNVLGRGVGIVDKASPFI*L
SFRMFLRCFHEYCR**YSVFIRISILGLNALFL
CTSFQVLTFNY*LISSSFLHIVGNCHIFAIMIGW
ACCCS**DILNSILSIKLDLTNKK

Frame 1

WLGRGGWGDSRQLRRESDESSALPPPSPGPALC
LPLQRPDDLSREGADSRGPGRSPAPPLLRPFPS
LTWPAAAAVTPPPAAAVAAAVTPSPAAAAAASAD
QGPARLRLLRT*RIKT*WEHF*TSQRWKSIMPR
GRVMGCDMG*AACKAGVLKWRMHIRL*SVCQVD
LNRGHSLLCMMGMLVLRLPNTAVSIC*ITSPITR
ILKGLQEHLLWKM*RMESEQVFWRLMNT*ELCQ
RRNMVQIEVGQQL*VS*FLPNILISLTVETQEV
YFVGTGKFISSHKITNQVIRWRKNEFRMQVAL**
FSV*MALWLYRGPLGILITNVSMEKVLLSSLSH
QSLKSMILKDLKKMISSLSLHVMVSGMLWEMKS
SVIL*DPDLKSLMTLRKFAMK*STPVCIREVETT
*V*F*SVFQMHPKYRQKQ*RRRQSWTSTWNAE*
KKS*RSRGKASPT*SM*CAH*RVRTSPASHQGV
NWQARGMLLKPFTID*ILTKMTTLTLHQQMICGK
TAHLAMEFTFTSKGEYSSTLLKLLTSILNFKEG
DMTWVRMITSENFSSTTASPELIFFFFFVNLRL
M*A*FQTIIRVVNQTPAIFVV*FCFFVKCNCPCT
KCSYLIMNCFKSLSKLQEMFGLLCDATDI*PFQ
VMLCLDLGLEQGEQQPCQLHAQMCR*LWKITSK
SYKAEHPRSY*KLS*MFLVGELALLIKPVPSFNC
LSGCSFVVSMSIAGNNTVYS*ESQSWG*MPCFF
APLFKSLHLITN**AAASYI**ETATFLLS*LA
GPAAVPSKIF*IPFYQ*SLI*QTR

Frame 2

GWGGGGGGTLDS*GAKAMSPRLFLLLLRDPLSA
SLSNARMI*AARAPTAGGPDAARLLPSSAPSPA
*PGPPLQR*PLPRLPPSPPR*PPPRLPPPPPRP
TRDLPACGCSGPRGSRHNGSIFRQAKDGKA*CPG
AG*WVAIWAKQHARLAC*NGGCTYGCDRFAKWT
*IVVILCCV*WACWFSGCQILL*AFVRSHHQ*P
GF*RVCRSTFCGKCKEWNQNRFSGD**THESYVR
EETWCR*KWVNSCRCLNFSPTYLFH*LWRLKRF
TL*EQESSFLHTRSQTK*SAGERTNSECRWLCN
DSACEWLSGCIEGPWGF*LQMCPWKRSY*AACLT
RA*SP*Y*KI*RR*SVHYPCM*WYLGCYGK*RA
L*FCKIQT*SH**P*ESLQ*SSRHLFV*GKSRQ
HECDFDLFSKCTQSIARSSEEGGRVGQVPGMQSR
RNHKEAGGRRPRLSPCDAHISE*EHPQPPTRG*
IGKQEECY*SRLQ*TESLQK*RH*LYINR*YVV
KLLI*PWSLPSPPKESTAQLC*NF*HPSSTLRKG
I*HG*E*LHQRTSAVQQLAQN*FFFFFL*I*DL
CKRDFKP*FVL*IRLQQFLLYDFVFL*SVIVLV
QNAHI*L*TALNHYQSYKKCLAYCVMQQIYSPFK
SCCVWTWGWNRESSSHVSYTLKCADDYGK*PQN
LTKLNIQGVIENYLKCSW*GSWHC**SQSLHLT
VFQDVPSLFP*VLQVIIQCIHKNLNLGAKCLVSL
HLFSSPYI*LLIDKQQLPTYSRKLPHFCYHDWL
GLLLFLVRYSEFHFINKA*FNKQE

Sequence NM_177951

GTGGTTGGGGAGGGGGGGGTGGGGGGACTCTAGACAGCTGAGGCGCGAAAGCGATGAGTCCTCGGCTCTTCCTCCTCCTTCTCCGGGACCCGCTCTCTGC
CTCCCTCTCCAACGCCCGGATGATCTGAGCCGCGAGGGCGCCGACAGCCGGGGGCCCGGACGCAGCCCGGCTCCTCCCCTCCTCCGCCCCTTCCCCAGCC
TGACCTGGCCCGCCGCTGCAGCGGTGACCCCTCCCCCGGCTGCCGCCGTCGCCGCCGCGGTGACCCCCTCCCCGGCTGCCGCCGCCGCCGCCTCGGCCGA
CCAGGGACCTGCCCGCCTGCGGCTGCTCCGGATTTTAAATAAACTTGGAGCCTTTTCTTGAAAGACAGCTGGCTGTGGAGAGCCTTCTACCAACTGCAGT
TTTAGATACGTTTTGAAAATTTCATCACCTGAGACCTAGAGGATCAAGACATAATGGGAGCATTTTTAGACAAGCCAAAGATGGAAAAGCATAATGCCCA
GGGGCAGGGTAATGGGTTGCGATATGGGCTAAGCAGCATGCAAGGCTGGCGTGTTGAAATGGAGGATGCACATACGGCTGTGATCGGTTTGCCAAGTGGA
CTTGAATCGTGGTCATTCTTTGCTGTGTATGATGGGCATGCTGGTTCTCAGGTTGCCAAATACTGCTGTGAGCATTTGTTAGATCACATCACCAATAACC
AGGATTTTAAAGGGTCTGCAGGAGCACCTTCTGTGGAAAATGTAAAGAATGGAATCAGAACAGGTTTTCTGGAGATTGATGAACACATGAGAGTTATGTC
AGAGAAGAAACATGGTGCAGATAGAAGTGGGTCAACAGCTGTAGGTGTCTTAATTTCTCCCCAACATACTTATTTCATTAACTGTGGAGACTCAAGAGGT
TTACTTTGTAGGAACAGGAAAGTTCATTTCTTCACACAAGATCACAAACCAAGTAATCCGCTGGAGAAAGAACGAATTCAGAATGCAGGTGGCTCTGTAA
TGATTCAGCGTGTGAATGGCTCTCTGGCTGTATCGAGGGCCCTTGGGGATTTTGATTACAAATGTGTCCATGGAAAAGGTCCTACTGAGCAGCTTGTCTC
ACCAGAGCCTGAAGTCCATGATATTGAAAGATCTGAAGAAGATGATCAGTTCATTATCCTTGCATGTGATGGTATCTGGGATGTTATGGGAAATGAAGAG
CTCTGTGATTTTGTAAGATCCAGACTTGAAGTCACTGATGACCTTGAGAAAGTTTGCAATGAAGTAGTCGACACCTGTTTGTATAAGGGAAGTCGAGACA
ACATGAGTGTGATTTTGATCTGTTTTCCAAATGCACCCAAAGTATCGCCAGAAGCAGTGAAGAAGGAGGCAGAGTTGGACAAGTACCTGGAATGCAGAGT
AGAAGGTGGATCATTTAACAAAAAATAAGTAGCTTTATTAAAGAAAAATCTTCAACACAATCAAACTTTAACATTTTAGCTTTTAGATTTTTATGTGTCT
TTGACAGCTAGTGTCTAGTGTATGAAAGTGGAAAAGGACATTTCTGTAGTAGCTGTTAAGATGTATGCAGTTGTCCTATCACAGTTATATTAGAAATAAC
AACTAGTGTGGAAAGTAACCCACATTCTGTTCAGGATACTTTTGTTTTAAAAGGCTAAAATGTTTGTGTGCTTTAAAATTCTTAATGAATTCACTGGCAT
GAGTAATTTTATGCCGGGAAAATCTGATTTGTGGTCTATTTAATTACAAAGAGATTGTGATGGTGATAATTTGAATACCATCTTTCTATGAAAAGCTGTG
TATATTAATTTTAATCTCTAGGGCCTTTTTTCAATAACTTGAAAATCACAGCCTCATCATTAGCAACTTTATATACACAGCTTTAAAAAATTTGCTTTAA
ACTGTTTCAGAGCTTCTTCCCTAAGCCATAAACTGTCTCACCTAAACAAGATAGACCTCAATAAAAGACATAATATTTCTCCACCTGGCAGAAAAGTAAC
TGCCAAGTAAGATTAAGAAATAAGTTGAGCATTTCTGGAGTATATTTCTCATAATTCTGCAGAGACTTCCAGTTTATTGCATATATGCAGTAAAGTGTAT
ATATTATGCAGTTTCTCTAAGTGGAAAAACTTGACATGACTTTAATAGTGTTAAAACATGAAAATGCTGGTGAGCTGAGGTAGAAAAAAAACAAGAAAAA
AATTTTAAAAATAGCGTTAAAATGCATTTTAGGCTCCTAATGTCGTTAATTTGCACGTTAAAGACCTCTAATCGCATGCAAGCATTGACCTACCACACCT
ACATCCCACTGTGCCTGCATTATTGAAATTACTACTCACTTTAATTTTTTTTCCCCTTTTTACTGTTTGATAC
  • refseq_PPM1A.F2 (forward primer)
  • refseq_PPM1A.R2 (reverse primer)
  • Single exon skipping

Frame 0

VVGEGGVGGL*TAEARKR*VLGSSSSFSGTRSL
PPSPTPG*SEPRGRRQPGARTQPGSSPPPPLPQP
DLARRCSGDPSPGCRRRRRGDPLPGCRRRRLGR
PGTCPPAAAPDFK*TWSLFLKDSWLWRAFYQLQ
F*IRFENFIT*DLEDQDIMGAFLDKPKMEKHNAQ
GQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSG
LESWSFFAVYDGHAGSQVAKYCCEHLLDHITNN
QDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMS
EKKHGADRSGSTAVGVLISPQHTYFINCGDSRG
LLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSV
MIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVS
PEPEVHDIERSEEDDQFIILACDGIWDVMGNEE
LCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRD
NMSVILICFPNAPKVSPEAVKKEAELDKYLECRV
EGGSFNKK*VALLKKNLQHNQTLTF*LLDFYVS
LTASV*CMKVEKDISVVAVKMYAVVLSQLY*K*
QLVWKVTHILFRILLF*KAKMFVCFKILNEFTGM
SNFMPGKSDLWSI*LQRDCDGDNLNTIFL*KAV
YINFNL*GLFSIT*KSQPHH*QLYIHSFKKFAL
NCFRASSLSHKLSHLNKIDLNKRHNISPPGRKVT
AK*D*EIS*AFLEYISHNSAETSSLLHICSKVY
ILCSFSKWKNLT*L**C*NMKMLVS*GRKKTRK
KF*K*R*NAF*APNVVNLHVKDL*SHASIDLPHL
HPTVPALLKLLLTLIFFPLFTV*Y

Frame 1

WLGRGGWGDSRQLRRESDESSALPPPSPGPALC
LPLQRPDDLSREGADSRGPGRSPAPPLLRPFPS
LTWPAAAAVTPPPAAAVAAAVTPSPAAAAAASAD
QGPARLRLLRILNKLGAFS*KTAGCGEPSTNCS
FRYVLKISSPET*RIKT*WEHF*TSQRWKSIMP
RGRVMGCDMG*AACKAGVLKWRMHIRL*SVCQVD
LNRGHSLLCMMGMLVLRLPNTAVSIC*ITSPIT
RILKGLQEHLLWKM*RMESEQVFWRLMNT*ELC
QRRNMVQIEVGQQL*VS*FLPNILISLTVETQEV
YFVGTGKFISSHKITNQVIRWRKNEFRMQVAL*
*FSV*MALWLYRGPLGILITNVSMEKVLLSSLS
HQSLKSMILKDLKKMISSLSLHVMVSGMLWEMKS
SVIL*DPDLKSLMTLRKFAMK*STPVCIREVET
T*V*F*SVFQMHPKYRQKQ*RRRQSWTSTWNAE
*KVDHLTKNK*LY*RKIFNTIKL*HFSF*IFMCL
*QLVSSV*KWKRTFL**LLRCMQLSYHSYIRNN
N*CGK*PTFCSGYFCFKRLKCLCALKFLMNSLA
*VILCRENLICGLFNYKEIVMVII*IPSFYEKLC
ILILISRAFFQ*LENHSLIISNFIYTALKNLL*
TVSELLP*AINCLT*TR*TSIKDIIFLHLAEK*
LPSKIKK*VEHFWSIFLIILQRLPVYCIYAVKCI
YYAVSLSGKT*HDFNSVKT*KCW*AEVEKKQEK
NFKNSVKMHFRLLMSLICTLKTSNRMQALTYHT
YIPLCLHY*NYYSL*FFFPFLLFD

Frame 2

GWGGGGGGTLDS*GAKAMSPRLFLLLLRDPLSA
SLSNARMI*AARAPTAGGPDAARLLPSSAPSPA
*PGPPLQR*PLPRLPPSPPR*PPPRLPPPPPRP
TRDLPACGCSGF*INLEPFLERQLAVESLLPTAV
LDTF*KFHHLRPRGSRHNGSIFRQAKDGKA*CP
GAG*WVAIWAKQHARLAC*NGGCTYGCDRFAKW
T*IVVILCCV*WACWFSGCQILL*AFVRSHHQ*P
GF*RVCRSTFCGKCKEWNQNRFSGD**THESYV
REETWCR*KWVNSCRCLNFSPTYLFH*LWRLKR
FTL*EQESSFLHTRSQTK*SAGERTNSECRWLCN
DSACEWLSGCIEGPWGF*LQMCPWKRSY*AACL
TRA*SP*Y*KI*RR*SVHYPCM*WYLGCYGK*R
AL*FCKIQT*SH**P*ESLQ*SSRHLFV*GKSRQ
HECDFDLFSKCTQSIARSSEEGGRVGQVPGMQS
RRWII*QKISSFIKEKSSTQSNFNILAFRFLCV
FDS*CLVYESGKGHFCSSC*DVCSCPITVILEIT
TSVESNPHSVQDTFVLKG*NVCVL*NS**IHWH
E*FYAGKI*FVVYLITKRL*W**FEYHLSMKSC
VY*F*SLGPFFNNLKITASSLATLYTQL*KICFK
LFQSFFPKP*TVSPKQDRPQ*KT*YFSTWQKSN
CQVRLRNKLSISGVYFS*FCRDFQFIAYMQ*SV
YIMQFL*VEKLDMTLIVLKHENAGELR*KKNKKK
ILKIALKCILGS*CR*FAR*RPLIACKH*PTTP
TSHCACIIEITTHFNFFSPFYCLI

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