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Sequences

Sequence NM_003988

AGGCTCCAGTCTCCGGCCGAGTCTTCTCGCAGCCGCAACCCACCTGGGGCCAGCCCAGAGCTGCCAGCGCCGCTCGGCTCCCTCCCTCCCTCCCGGCCCT TCGGCCGCGGCGGCGTGCGCCTGCCTTTTCCGGGGGCGGGGGCCTGGCCCGCGCGCTCCCCTCCCGCAGGCGCCACCTCGGACATCCCCGGGATTGCTAC TTCTCTGCCAACTTCGCCAACTCGCCAGCACTTGGAGAGGCCCGGCTCCCCTCCCGGCGCCCTCTGACCGCCCCCGCCCCGCGCGCTCTCCGACCACCGC CTCTCGGATGACCAGGTTCCAGGGGAGCTGAGCGAGTCGCCTCCCCCGCCCAGCTTCAGCCCTGGCTGCAGCTGCAGCGCGAGCCATGCGCCCCCAGTGC ACCCCGGCCCGGCCCACCGCCCCGGGGCCATTCTGCTGACCGCCCAGCCCCGAGCCCCGACAGTGGCAAGTTGCGGCTACTGCAGTTGCAAGCTCCGGCC AACCCGGAGGAGCCCCAGCGGGGAGCGCAGTGCTGCGCCCCCCGCCCCCGCGCGCCCCGCAGCAGCCGGGCGTTCACTCATCCTCCCTCCCCCACCGTCC CTCCCTTTTCTCCTCAAGTCCTGAAGTTGAGTTTGAGAGGCGACACGGCGGCGGCGGCCGCGCTGCTCCCGCTCCTCTGCCTCCCCATGGATATGCACTG CAAAGCAGACCCCTTCTCCGCGATGCACCCAGGGCACGGGGGTGTGAACCAGCTCGGGGGGGTGTTTGTGAACGGCCGGCCCCTACCCGACGTGGTGAGG CAGCGCATCGTGGAGCTGGCCCACCAGGGTGTGCGGCCCTGTGACATCTCCCGGCAGCTGCGGGTCAGCCACGGCTGTGTCAGCAAAATCCTGGGCAGGT ACTACGAGACCGGCAGCATCAAGCCGGGTGTGATCGGTGGCTCCAAGCCCAAAGTGGCGACGCCCAAAGTGGTGGACAAGATTGCTGAATACAAACGACA GAACCCGACTATGTTCGCCTGGGAGATTCGAGACCGGCTCCTGGCCGAGGGCATCTGTGACAATGACACAGTGCCCAGCGTCTCTTCCATCAACAGAATC ATCCGGACCAAAGTTCAGCAGCCTTTCCACCCAACGCCGGATGGGGCTGGGACAGGAGTGACCGCCCCTGGCCACACCATTGTTCCCAGCACGGCCTCCC CTCCTGTTTCCAGCGCCTCCAATGACCCAGTGGGATCCTACTCCATCAATGGGATCCTGGGGATTCCTCGCTCCAATGGTGAGAAGAGGAAACGTGATGA AGATGTGTCTGAGGGCTCAGTCCCCAATGGAGATTCCCAGAGTGGTGTGGACAGTTTGCGGAAGCACTTGCGAGCTGACACCTTCACCCAGCAGCAGCTG GAAGCTTTGGATCGGGTCTTTGAGCGTCCTTCCTACCCTGACGTCTTCCAGGCATCAGAGCACATCAAATCAGAACAGGGGAACGAGTACTCCCTCCCAG CCCTGACCCCTGGGCTTGATGAAGTCAAGTCGAGTCTATCTGCATCCACCAACCCTGAGCTGGGCAGCAACGTGTCAGGCACACAGACATACCCAGTTGT GACTGGTCGTGACATGGCGAGCACCACTCTGCCTGGTTACCCCCCTCACGTGCCCCCCACTGGCCAGGGAAGCTACCCCACCTCCACCCTGGCAGGAATG GTGCCTGAGGCTGCAGTTGGTCCCTCATCCTCCCTCATGAGCAAGCCGGGGAGGAAGCTTGCAGAAGTGCCCCCTTGTGTGCAACCCACTGGAGCGAGTT CTCCGGCAACCCGTACAGCCACCCCCAGTACACGGCCTACAACGAGGCTTGGAGATTCAGCAACCCCGCCTTACTAAGTTCCCCTTATTATTATAGTGCC GCCCCCCGGGGCTCCGCCCCTGCCGCTGCTGCCGCTGCCTATGACCGCCACTAGTTACCGCGGGGACCACATCAAGCTTCAGGCCGACAGCTTCGGCCTC CACATCGTCCCCGTCTGACCCCACCCCGGAGGGAGGGAGGACCGACGCGACGCGATGCCTCCCGGCCACCGCCCCAGCCTCACCCCATCCCACGACCCCC GCAACCCTTCACATCACCCCCCTCGAAGGTCGGACAGGACGGGTGGAGCCGTGGGCGGGACCCTCAGGCCCGGGCCCGCCGCCCCCAGCCCCGCCTGCCG CCCCTCCCCGCCTGCCTGGACTGCGCGGCGCCGTGAGGGGGATTCGGCCCAGCTCGTCCCGGCCTCCACCAAGCCAGCCCCGAAGCCCGCCAGCCACCCT GCCGGACTCGGGCGCGACCTGCTGGCGCGCGCCGGATGTTTCTGTGACACACAATCAGCGCGGACCGCAGCGCGGCCCAGCCCCGGGCACCCGCCTCGGA CGCTCGGGCGCCAGGAGGCTTCGCTGGAGGGGCTGGGCCAAGGAGATTAAGAAGAAAACGACTTTCTGCAGGAGGAAGAGCCCGCTGCCGAATCCCTGGG AAAAATTCTTTTCCCCCAGTGCCAGCCGGACTGCCCTCGCCTTCCGGGTGTGCCCTGTCCCAGAAGATGGAATGGGGGTGTGGGGGTCCGGCTCTAGGAA CGGGCTTTGGGGGCGTCAGGTCTTTCCAAGGTTGGGACCCAAGGATCGGGGGGCCCAGCAGCCCGCACCGATCGAGCCGGACTCTCGGCTCTTCACTGCT CCTCCTGGCCTGCCTAGTTCCCCAGGGCCCGGCACCTCCTGCTGCGAGACCCGGCTCTCAGCCCTGCCTTGCCCCTACCTCAGCGTCTCTTCCACCTGCT GGCCTCCCAGTTTCCCCTCCTGCCAGTCCTTCGCCTGTCCCTTGACGCCCTGCATCCTCCTCCCTGACTCGCAGCCCCATCGGACGCTCTCCCGGGACCG CCGCAGGACCAGTTTCCATAGACTGCGGACTGGGGTCTTCCTCCAGCAGTTACTTGATGCCCCCTCCCCCGACACAGACTCTCAATCTGCCGGTGGTAAG AACCGGTTCTGAGCTGGCGTCTGAGCTGCTGCGGGGTGGAAGTGGGGGGCTGCCCACTCCACTCCTCCCATCCCCTCCCAGCCTCCTCCTCCGGCAGGAA CTGAACAGAACCACAAAAAGTCTACATTTATTTAATATGATGGTCTTTGCAAAAAGGAACAAAACAACACAAAAGCCCACCAGGCTGCTGCTTTGTGGAA AGACGGTGTGTGTCGTGTGAAGGCGAAACCCGGTGTACATAACCCCTCCCCCTCCGCCCCGCCCCGCCCGGCCCCGTAGAGTCCCTGTCGCCCGCCGGCC CTGCCTGTAGATACGCCCCGCTGTCTGTGCTGTGAGAGTCGCCGCTCGCTGGGGGGGAAGGGGGGGACACAGCTACACGCCCATTAAAGCACAGCACGTC CTGGGGGAGGGGGGCATTTTTTATGTTACAAAAAAAAATTACGAAAGAAAAGAAATCTCTATGCAAAATGACGAACATGGTCCTGTGGACTCCTCTGGCC TGTTTTGTTGGCTCTTTCTCTGTAATTCCGTGTTTTCGCTTTTTCCTCCCTGCCCCTCTCTCCCTCTGCCCCTCTCTCCTCTCCGCTTCTCTCCCCCTCT GTCTCTGTCTCTCTCCGTCTCTGTCGCTCTTGTCTGTCTGTCTCTGCTCTTTCCTCGGCCTCTCTCCCCAGACCTGGCCCGGCCGCCCTGTCTCCGCAGG CTAGATCCGAGGTGGCAGCTCCAGCCCCCGGGCTCGCCCCCTCGCGGGCGTGCCCCGCGCGCCCCGGGCGGCCGAAGGCCGGGCCGCCCCGTCCCGCCCC GTAGTTGCTCTTTCGGTAGTGGCGATGCGCCCTGCATGTCTCCTCACCCGTGGATCGTGACGACTCGAAATAACAGAAACAAAGTCAATAAAGTGAAAAT AAATAAAAATCCTTGAACAAATCCGAAAAGGCTTGGAGTCCTCGCCCAGATCTCTCTCCCCTGCGAGCCCTTTTTATTTGAGAAGGAAAAAGAGAAAAGA GAATCGTTTAAGGGAACCCGGCGCCCAGCCAGGCTCCAGTGGCCCGAACGGGGCGGCGAGGGCGGCGAGGGCGCCGAGGTCCGGCCCATCCCAGTCCTGT GGGGCTGGCCGGGCAGAGACCCCGGACCCAGGCCCAGGCCTAACCTGCTAAATGTCCCCGGACGGTTCTGGTCTCCTCGGCCACTTTCAGTGCGTCGGTT CGTTTTGATTCTTTTTCTTTTGTGCACATAAGAAATAAATAATAATAATAAATAAAGAATAAAATTTTGTATGTCA

refseq_PAX2.F5 (forward primer)
refseq_PAX2.R5 (reverse primer)
Single exon skipping

Frame 0

RLQSPAESSRSRNPPGASPELPAPLGSLPPSRP FGRGGVRLPFPGAGAWPARSPPAGATSDIPGIAT SLPTSPTRQHLERPGSPPGAL*PPPPRALSDHR LSDDQVPGELSESPPPPSFSPGCSCSASHAPPV HPGPAHRPGAILLTAQPRAPTVASCGYCSCKLRP TRRSPSGERSAAPPAPARPAAAGRSLILPPPPS LPFLLKS*S*V*EATRRRRPRCSRSSASPWICT AKQTPSPRCTQGTGV*TSSGGCL*TAGPYPTW*G SASWSWPTRVCGPVTSPGSCGSATAVSAKSWAG TTRPAASSRV*SVAPSPKWRRPKWWTRLLNTND RTRLCSPGRFETGSWPRASVTMTQCPASLPSTES SGPKFSSLSTQRRMGLGQE*PPLATPLFPARPP LLFPAPPMTQWDPTPSMGSWGFLAPMVRRGNVM KMCLRAQSPMEIPRVVWTVCGSTCELTPSPSSSW KLWIGSLSVLPTLTSSRHQSTSNQNRGTSTPSQ P*PLGLMKSSRVYLHPPTLSWAATCQAHRHTQL *LVVTWRAPLCLVTPLTCPPLAREATPPPPWQEW CLRLQLVPHPPS*ASRGGSLQKCPLVCNPLERV LRQPVQPPPVHGLQRGLEIQQPRLTKFPLLL*C RPPGLRPCRCCRCL*PPLVTAGTTSSFRPTASAS TSSPSDPTPEGGRTDATRCLPATAPASPHPTTP ATLHITPLEGRTGRVEPWAGPSGPGPPPPAPPA APPRLPGLRGAVRGIRPSSSRPPPSQPRSPPATL PDSGATCWRAPDVSVTHNQRGPQRGPAPGTRLG RSGARRLRWRGWAKEIKKKTTFCRRKSPLPNPW EKFFSPSASRTALAFRVCPVPEDGMGVWGSGSRN GLWGRQVFPRLGPKDRGAQQPAPIEPDSRLFTA PPGLPSSPGPGTSCCETRLSALPCPYLSVSSTC WPPSFPSCQSFACPLTPCILLPDSQPHRTLSRDR RRTSFHRLRTGVFLQQLLDAPSPDTDSQSAGGK NRF*AGV*AAAGWKWGAAHSTPPIPSQPPPPAG TEQNHKKSTFI*YDGLCKKEQNNTKAHQAAALWK DGVCRVKAKPGVHNPSPSAPPRPAP*SPCRPPA LPVDTPRCLCCESRRSLGGKGGTQLHAH*STAR PGGGGHFLCYKKKLRKKRNLYAK*RTWSCGLLWP VLLALSL*FRVFAFSSLPLSPSAPLSSPLLSPS VSVSLRLCRSCLSVSALSSASLPRPGPAALSPQ ARSEVAAPAPGLAPSRACPARPGRPKAGPPRPAP *LLFR*WRCALHVSSPVDRDDSK*QKQSQ*SEN K*KSLNKSEKAWSPRPDLSPLRALFI*EGKREK RIV*GNPAPSQAPVARTGRRGRRGRRGPAHPSPV GLAGQRPRTQAQA*PAKCPRTVLVSSATFSASV RFDSFSFVHIRNK****IKNKILYV

Frame 1

GSSLRPSLLAAATHLGPAQSCQRRSAPSLPPGP SAAAACACLFRGRGPGPRAPLPQAPPRTSPGLL LLCQLRQLASTWRGPAPLPAPSDRPRPARSPTTA SRMTRFQGS*ASRLPRPASALAAAAARAMRPQC TPARPTAPGPFC*PPSPEPRQWQVAATAVASSG QPGGAPAGSAVLRPPPPRAPQQPGVHSSSLPHRP SLFSSSPEVEFERRHGGGGRAAPAPLPPHGYAL QSRPLLRDAPRARGCEPARGGVCERPAPTRRGE AAHRGAGPPGCAAL*HLPAAAGQPRLCQQNPGQV LRDRQHQAGCDRWLQAQSGDAQSGGQDC*IQTT EPDYVRLGDSRPAPGRGHL*Q*HSAQRLFHQQN HPDQSSAAFPPNAGWGWDRSDRPWPHHCSQHGLP SCFQRLQ*PSGILLHQWDPGDSSLQW*EEET** RCV*GLSPQWRFPEWCGQFAEALAS*HLHPAAA GSFGSGL*ASFLP*RLPGIRAHQIRTGERVLPPS PDPWA**SQVESICIHQP*AGQQRVRHTDIPSC DWS*HGEHHSAWLPPSRAPHWPGKLPHLHPGRN GA*GCSWSLILPHEQAGEEACRSAPLCATHWSEF SGNPYSHPQYTAYNEAWRFSNPALLSSPYYYSA APRGSAPAAAAAAYDRH*LPRGPHQASGRQLRP PHRPRLTPPRREGGPTRRDASRPPPQPHPIPRPP QPFTSPPSKVGQDGWSRGRDPQARARRPQPRLP PLPACLDCAAP*GGFGPARPGLHQASPEARQPP CRTRARPAGARRMFL*HTISADRSAAQPRAPASD ARAPGGFAGGAGPRRLRRKRLSAGGRARCRIPG KNSFPPVPAGLPSPSGCALSQKMEWGCGGPALG TGFGGVRSFQGWDPRIGGPSSPHRSSRTLGSSLL LLACLVPQGPAPPAARPGSQPCLAPTSASLPPA GLPVSPPASPSPVP*RPASSSLTRSPIGRSPGT AAGPVSIDCGLGSSSSSYLMPPPPTQTLNLPVVR TGSELASELLRGGSGGLPTPLLPSPPSLLLRQE LNRTTKSLHLFNMMVFAKRNKTTQKPTRLLLCG KTVCVV*RRNPVYITPPPPPRPARPRRVPVARRP CL*IRPAVCAVRVAARWGGRGGHSYTPIKAQHV LGEGGIFYVTKKNYERKEISMQNDEHGPVDSSG LFCWLFLCNSVFSLFPPCPSLPLPLSPLRFSPPL SLSLSVSVALVCLSLLFPRPLSPDLARPPCLRR LDPRWQLQPPGSPPRGRAPRAPGGRRPGRPVPP RSCSFGSGDAPCMSPHPWIVTTRNNRNKVNKVKI NKNP*TNPKRLGVLAQISLPCEPFLFEKEKEKR ESFKGTRRPARLQWPERGGEGGEGAEVRPIPVL WGWPGRDPGPRPRPNLLNVPGRFWSPRPLSVRRF VLILFLLCT*EINNNNK*RIKFCMS

Frame 2

APVSGRVFSQPQPTWGQPRAASAARLPPSLPAL RPRRRAPAFSGGGGLARALPSRRRHLGHPRDCY FSANFANSPALGEARLPSRRPLTAPAPRALRPP PLG*PGSRGAERVASPAQLQPWLQLQREPCAPSA PRPGPPPRGHSADRPAPSPDSGKLRLLQLQAPA NPEEPQRGAQCCAPRPRAPRSSRAFTHPPSPTV PPFSPQVLKLSLRGDTAAAAALLPLLCLPMDMHC KADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQLRVSHGCVSKILGR YYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQ NPTMFAWEIRDRLLAEGICDNDTVPSVSSINRI IRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTAS PPVSSASNDPVGSYSINGILGIPRSNGEKRKRDE DVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQL EALDRVFERPSYPDVFQASEHIKSEQGNEYSLP ALTPGLDEVKSSLSASTNPELGSNVSGTQTYPVV TGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGM VPEAAVGPSSSLMSKPGRKLAEVPPCVQPTGAS SPATRTATPSTRPTTRLGDSATPPY*VPLIIIVP PPGAPPLPLLPLPMTATSYRGDHIKLQADSFGL HIVPV*PHPGGREDRRDAMPPGHRPSLTPSHDP RNPSHHPPRRSDRTGGAVGGTLRPGPAAPSPACR PSPPAWTARRREGDSAQLVPASTKPAPKPASHP AGLGRDLLARAGCFCDTQSARTAARPSPGHPPR TLGRQEASLEGLGQGD*EENDFLQEEEPAAESLG KILFPQCQPDCPRLPGVPCPRRWNGGVGVRL*E RALGASGLSKVGTQGSGGPAARTDRAGLSALHC SSWPA*FPRARHLLLRDPALSPALPLPQRLFHLL ASQFPLLPVLRLSLDALHPPP*LAAPSDALPGP PQDQFP*TADWGLPPAVT*CPLPRHRLSICRW* EPVLSWRLSCCGVEVGGCPLHSSHPLPASSSGRN *TEPQKVYIYLI*WSLQKGTKQHKSPPGCCFVE RRCVSCEGETRCT*PLPLRPAPPGPVESLSPAG PACRYAPLSVL*ESPLAGGEGGDTATRPLKHSTS WGRGAFFMLQKKITKEKKSLCKMTNMVLWTPLA CFVGSFSVIPCFRFFLPAPLSLCPSLLSASLPL CLCLSPSLSLLSVCLCSFLGLSPQTWPGRPVSAG *IRGGSSSPRARPLAGVPRAPRAAEGRAAPSRP VVALSVVAMRPACLLTRGS*RLEITETKSIK*K *IKILEQIRKGLESSPRSLSPASPFYLRRKKRKE NRLREPGAQPGSSGPNGAARAARAPRSGPSQSC GAGRAETPDPGPGLTC*MSPDGSGLLGHFQCVG SF*FFFFCAHKK*IIIINKE*NFVC

Sequence NM_000278

AGGCTCCAGTCTCCGGCCGAGTCTTCTCGCAGCCGCAACCCACCTGGGGCCAGCCCAGAGCTGCCAGCGCCGCTCGGCTCCCTCCCTCCCTCCCGGCCCT TCGGCCGCGGCGGCGTGCGCCTGCCTTTTCCGGGGGCGGGGGCCTGGCCCGCGCGCTCCCCTCCCGCAGGCGCCACCTCGGACATCCCCGGGATTGCTAC TTCTCTGCCAACTTCGCCAACTCGCCAGCACTTGGAGAGGCCCGGCTCCCCTCCCGGCGCCCTCTGACCGCCCCCGCCCCGCGCGCTCTCCGACCACCGC CTCTCGGATGACCAGGTTCCAGGGGAGCTGAGCGAGTCGCCTCCCCCGCCCAGCTTCAGCCCTGGCTGCAGCTGCAGCGCGAGCCATGCGCCCCCAGTGC ACCCCGGCCCGGCCCACCGCCCCGGGGCCATTCTGCTGACCGCCCAGCCCCGAGCCCCGACAGTGGCAAGTTGCGGCTACTGCAGTTGCAAGCTCCGGCC AACCCGGAGGAGCCCCAGCGGGGAGCGCAGTGCTGCGCCCCCCGCCCCCGCGCGCCCCGCAGCAGCCGGGCGTTCACTCATCCTCCCTCCCCCACCGTCC CTCCCTTTTCTCCTCAAGTCCTGAAGTTGAGTTTGAGAGGCGACACGGCGGCGGCGGCCGCGCTGCTCCCGCTCCTCTGCCTCCCCATGGATATGCACTG CAAAGCAGACCCCTTCTCCGCGATGCACCCAGGGCACGGGGGTGTGAACCAGCTCGGGGGGGTGTTTGTGAACGGCCGGCCCCTACCCGACGTGGTGAGG CAGCGCATCGTGGAGCTGGCCCACCAGGGTGTGCGGCCCTGTGACATCTCCCGGCAGCTGCGGGTCAGCCACGGCTGTGTCAGCAAAATCCTGGGCAGGT ACTACGAGACCGGCAGCATCAAGCCGGGTGTGATCGGTGGCTCCAAGCCCAAAGTGGCGACGCCCAAAGTGGTGGACAAGATTGCTGAATACAAACGACA GAACCCGACTATGTTCGCCTGGGAGATTCGAGACCGGCTCCTGGCCGAGGGCATCTGTGACAATGACACAGTGCCCAGCGTCTCTTCCATCAACAGAATC ATCCGGACCAAAGTTCAGCAGCCTTTCCACCCAACGCCGGATGGGGCTGGGACAGGAGTGACCGCCCCTGGCCACACCATTGTTCCCAGCACGGCCTCCC CTCCTGTTTCCAGCGCCTCCAATGACCCAGTGGGATCCTACTCCATCAATGGGATCCTGGGGATTCCTCGCTCCAATGGTGAGAAGAGGAAACGTGATGA AGATGTGTCTGAGGGCTCAGTCCCCAATGGAGATTCCCAGAGTGGTGTGGACAGTTTGCGGAAGCACTTGCGAGCTGACACCTTCACCCAGCAGCAGCTG GAAGCTTTGGATCGGGTCTTTGAGCGTCCTTCCTACCCTGACGTCTTCCAGGCATCAGAGCACATCAAATCAGAACAGGGGAACGAGTACTCCCTCCCAG CCCTGACCCCTGGGCTTGATGAAGTCAAGTCGAGTCTATCTGCATCCACCAACCCTGAGCTGGGCAGCAACGTGTCAGGCACACAGACATACCCAGTTGT GACTGGTCGTGACATGGCGAGCACCACTCTGCCTGGTTACCCCCCTCACGTGCCCCCCACTGGCCAGGGAAGCTACCCCACCTCCACCCTGGCAGGAATG GTGCCTGGGAGCGAGTTCTCCGGCAACCCGTACAGCCACCCCCAGTACACGGCCTACAACGAGGCTTGGAGATTCAGCAACCCCGCCTTACTAAGTTCCC CTTATTATTATAGTGCCGCCCCCCGGGGCTCCGCCCCTGCCGCTGCTGCCGCTGCCTATGACCGCCACTAGTTACCGCGGGGACCACATCAAGCTTCAGG CCGACAGCTTCGGCCTCCACATCGTCCCCGTCTGACCCCACCCCGGAGGGAGGGAGGACCGACGCGACGCGATGCCTCCCGGCCACCGCCCCAGCCTCAC CCCATCCCACGACCCCCGCAACCCTTCACATCACCCCCCTCGAAGGTCGGACAGGACGGGTGGAGCCGTGGGCGGGACCCTCAGGCCCGGGCCCGCCGCC CCCAGCCCCGCCTGCCGCCCCTCCCCGCCTGCCTGGACTGCGCGGCGCCGTGAGGGGGATTCGGCCCAGCTCGTCCCGGCCTCCACCAAGCCAGCCCCGA AGCCCGCCAGCCACCCTGCCGGACTCGGGCGCGACCTGCTGGCGCGCGCCGGATGTTTCTGTGACACACAATCAGCGCGGACCGCAGCGCGGCCCAGCCC CGGGCACCCGCCTCGGACGCTCGGGCGCCAGGAGGCTTCGCTGGAGGGGCTGGGCCAAGGAGATTAAGAAGAAAACGACTTTCTGCAGGAGGAAGAGCCC GCTGCCGAATCCCTGGGAAAAATTCTTTTCCCCCAGTGCCAGCCGGACTGCCCTCGCCTTCCGGGTGTGCCCTGTCCCAGAAGATGGAATGGGGGTGTGG GGGTCCGGCTCTAGGAACGGGCTTTGGGGGCGTCAGGTCTTTCCAAGGTTGGGACCCAAGGATCGGGGGGCCCAGCAGCCCGCACCGATCGAGCCGGACT CTCGGCTCTTCACTGCTCCTCCTGGCCTGCCTAGTTCCCCAGGGCCCGGCACCTCCTGCTGCGAGACCCGGCTCTCAGCCCTGCCTTGCCCCTACCTCAG CGTCTCTTCCACCTGCTGGCCTCCCAGTTTCCCCTCCTGCCAGTCCTTCGCCTGTCCCTTGACGCCCTGCATCCTCCTCCCTGACTCGCAGCCCCATCGG ACGCTCTCCCGGGACCGCCGCAGGACCAGTTTCCATAGACTGCGGACTGGGGTCTTCCTCCAGCAGTTACTTGATGCCCCCTCCCCCGACACAGACTCTC AATCTGCCGGTGGTAAGAACCGGTTCTGAGCTGGCGTCTGAGCTGCTGCGGGGTGGAAGTGGGGGGCTGCCCACTCCACTCCTCCCATCCCCTCCCAGCC TCCTCCTCCGGCAGGAACTGAACAGAACCACAAAAAGTCTACATTTATTTAATATGATGGTCTTTGCAAAAAGGAACAAAACAACACAAAAGCCCACCAG GCTGCTGCTTTGTGGAAAGACGGTGTGTGTCGTGTGAAGGCGAAACCCGGTGTACATAACCCCTCCCCCTCCGCCCCGCCCCGCCCGGCCCCGTAGAGTC CCTGTCGCCCGCCGGCCCTGCCTGTAGATACGCCCCGCTGTCTGTGCTGTGAGAGTCGCCGCTCGCTGGGGGGGAAGGGGGGGACACAGCTACACGCCCA TTAAAGCACAGCACGTCCTGGGGGAGGGGGGCATTTTTTATGTTACAAAAAAAAATTACGAAAGAAAAGAAATCTCTATGCAAAATGACGAACATGGTCC TGTGGACTCCTCTGGCCTGTTTTGTTGGCTCTTTCTCTGTAATTCCGTGTTTTCGCTTTTTCCTCCCTGCCCCTCTCTCCCTCTGCCCCTCTCTCCTCTC CGCTTCTCTCCCCCTCTGTCTCTGTCTCTCTCCGTCTCTGTCGCTCTTGTCTGTCTGTCTCTGCTCTTTCCTCGGCCTCTCTCCCCAGACCTGGCCCGGC CGCCCTGTCTCCGCAGGCTAGATCCGAGGTGGCAGCTCCAGCCCCCGGGCTCGCCCCCTCGCGGGCGTGCCCCGCGCGCCCCGGGCGGCCGAAGGCCGGG CCGCCCCGTCCCGCCCCGTAGTTGCTCTTTCGGTAGTGGCGATGCGCCCTGCATGTCTCCTCACCCGTGGATCGTGACGACTCGAAATAACAGAAACAAA GTCAATAAAGTGAAAATAAATAAAAATCCTTGAACAAATCCGAAAAGGCTTGGAGTCCTCGCCCAGATCTCTCTCCCCTGCGAGCCCTTTTTATTTGAGA AGGAAAAAGAGAAAAGAGAATCGTTTAAGGGAACCCGGCGCCCAGCCAGGCTCCAGTGGCCCGAACGGGGCGGCGAGGGCGGCGAGGGCGCCGAGGTCCG GCCCATCCCAGTCCTGTGGGGCTGGCCGGGCAGAGACCCCGGACCCAGGCCCAGGCCTAACCTGCTAAATGTCCCCGGACGGTTCTGGTCTCCTCGGCCA CTTTCAGTGCGTCGGTTCGTTTTGATTCTTTTTCTTTTGTGCACATAAGAAATAAATAATAATAATAAATAAAGAATAAAATTTTGTATGTCA

refseq_PAX2.F5 (forward primer)
refseq_PAX2.R5 (reverse primer)
Single exon skipping

Frame 0

RLQSPAESSRSRNPPGASPELPAPLGSLPPSRP FGRGGVRLPFPGAGAWPARSPPAGATSDIPGIAT SLPTSPTRQHLERPGSPPGAL*PPPPRALSDHR LSDDQVPGELSESPPPPSFSPGCSCSASHAPPV HPGPAHRPGAILLTAQPRAPTVASCGYCSCKLRP TRRSPSGERSAAPPAPARPAAAGRSLILPPPPS LPFLLKS*S*V*EATRRRRPRCSRSSASPWICT AKQTPSPRCTQGTGV*TSSGGCL*TAGPYPTW*G SASWSWPTRVCGPVTSPGSCGSATAVSAKSWAG TTRPAASSRV*SVAPSPKWRRPKWWTRLLNTND RTRLCSPGRFETGSWPRASVTMTQCPASLPSTES SGPKFSSLSTQRRMGLGQE*PPLATPLFPARPP LLFPAPPMTQWDPTPSMGSWGFLAPMVRRGNVM KMCLRAQSPMEIPRVVWTVCGSTCELTPSPSSSW KLWIGSLSVLPTLTSSRHQSTSNQNRGTSTPSQ P*PLGLMKSSRVYLHPPTLSWAATCQAHRHTQL *LVVTWRAPLCLVTPLTCPPLAREATPPPPWQEW CLGASSPATRTATPSTRPTTRLGDSATPPY*VP LIIIVPPPGAPPLPLLPLPMTATSYRGDHIKLQ ADSFGLHIVPV*PHPGGREDRRDAMPPGHRPSLT PSHDPRNPSHHPPRRSDRTGGAVGGTLRPGPAA PSPACRPSPPAWTARRREGDSAQLVPASTKPAP KPASHPAGLGRDLLARAGCFCDTQSARTAARPSP GHPPRTLGRQEASLEGLGQGD*EENDFLQEEEP AAESLGKILFPQCQPDCPRLPGVPCPRRWNGGV GVRL*ERALGASGLSKVGTQGSGGPAARTDRAGL SALHCSSWPA*FPRARHLLLRDPALSPALPLPQ RLFHLLASQFPLLPVLRLSLDALHPPP*LAAPS DALPGPPQDQFP*TADWGLPPAVT*CPLPRHRLS ICRW*EPVLSWRLSCCGVEVGGCPLHSSHPLPA SSSGRN*TEPQKVYIYLI*WSLQKGTKQHKSPP GCCFVERRCVSCEGETRCT*PLPLRPAPPGPVES LSPAGPACRYAPLSVL*ESPLAGGEGGDTATRP LKHSTSWGRGAFFMLQKKITKEKKSLCKMTNMV LWTPLACFVGSFSVIPCFRFFLPAPLSLCPSLLS ASLPLCLCLSPSLSLLSVCLCSFLGLSPQTWPG RPVSAG*IRGGSSSPRARPLAGVPRAPRAAEGR AAPSRPVVALSVVAMRPACLLTRGS*RLEITETK SIK*K*IKILEQIRKGLESSPRSLSPASPFYLR RKKRKENRLREPGAQPGSSGPNGAARAARAPRS GPSQSCGAGRAETPDPGPGLTC*MSPDGSGLLGH FQCVGSF*FFFFCAHKK*IIIINKE*NFVC

Frame 1

GSSLRPSLLAAATHLGPAQSCQRRSAPSLPPGP SAAAACACLFRGRGPGPRAPLPQAPPRTSPGLL LLCQLRQLASTWRGPAPLPAPSDRPRPARSPTTA SRMTRFQGS*ASRLPRPASALAAAAARAMRPQC TPARPTAPGPFC*PPSPEPRQWQVAATAVASSG QPGGAPAGSAVLRPPPPRAPQQPGVHSSSLPHRP SLFSSSPEVEFERRHGGGGRAAPAPLPPHGYAL QSRPLLRDAPRARGCEPARGGVCERPAPTRRGE AAHRGAGPPGCAAL*HLPAAAGQPRLCQQNPGQV LRDRQHQAGCDRWLQAQSGDAQSGGQDC*IQTT EPDYVRLGDSRPAPGRGHL*Q*HSAQRLFHQQN HPDQSSAAFPPNAGWGWDRSDRPWPHHCSQHGLP SCFQRLQ*PSGILLHQWDPGDSSLQW*EEET** RCV*GLSPQWRFPEWCGQFAEALAS*HLHPAAA GSFGSGL*ASFLP*RLPGIRAHQIRTGERVLPPS PDPWA**SQVESICIHQP*AGQQRVRHTDIPSC DWS*HGEHHSAWLPPSRAPHWPGKLPHLHPGRN GAWERVLRQPVQPPPVHGLQRGLEIQQPRLTKFP LLL*CRPPGLRPCRCCRCL*PPLVTAGTTSSFR PTASASTSSPSDPTPEGGRTDATRCLPATAPAS PHPTTPATLHITPLEGRTGRVEPWAGPSGPGPPP PAPPAAPPRLPGLRGAVRGIRPSSSRPPPSQPR SPPATLPDSGATCWRAPDVSVTHNQRGPQRGPA PGTRLGRSGARRLRWRGWAKEIKKKTTFCRRKSP LPNPWEKFFSPSASRTALAFRVCPVPEDGMGVW GSGSRNGLWGRQVFPRLGPKDRGAQQPAPIEPD SRLFTAPPGLPSSPGPGTSCCETRLSALPCPYLS VSSTCWPPSFPSCQSFACPLTPCILLPDSQPHR TLSRDRRRTSFHRLRTGVFLQQLLDAPSPDTDS QSAGGKNRF*AGV*AAAGWKWGAAHSTPPIPSQP PPPAGTEQNHKKSTFI*YDGLCKKEQNNTKAHQ AAALWKDGVCRVKAKPGVHNPSPSAPPRPAP*S PCRPPALPVDTPRCLCCESRRSLGGKGGTQLHAH *STARPGGGGHFLCYKKKLRKKRNLYAK*RTWS CGLLWPVLLALSL*FRVFAFSSLPLSPSAPLSS PLLSPSVSVSLRLCRSCLSVSALSSASLPRPGPA ALSPQARSEVAAPAPGLAPSRACPARPGRPKAG PPRPAP*LLFR*WRCALHVSSPVDRDDSK*QKQ SQ*SENK*KSLNKSEKAWSPRPDLSPLRALFI*E GKREKRIV*GNPAPSQAPVARTGRRGRRGRRGP AHPSPVGLAGQRPRTQAQA*PAKCPRTVLVSSA TFSASVRFDSFSFVHIRNK****IKNKILYV

Frame 2

APVSGRVFSQPQPTWGQPRAASAARLPPSLPAL RPRRRAPAFSGGGGLARALPSRRRHLGHPRDCY FSANFANSPALGEARLPSRRPLTAPAPRALRPP PLG*PGSRGAERVASPAQLQPWLQLQREPCAPSA PRPGPPPRGHSADRPAPSPDSGKLRLLQLQAPA NPEEPQRGAQCCAPRPRAPRSSRAFTHPPSPTV PPFSPQVLKLSLRGDTAAAAALLPLLCLPMDMHC KADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQLRVSHGCVSKILGR YYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQ NPTMFAWEIRDRLLAEGICDNDTVPSVSSINRI IRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTAS PPVSSASNDPVGSYSINGILGIPRSNGEKRKRDE DVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQL EALDRVFERPSYPDVFQASEHIKSEQGNEYSLP ALTPGLDEVKSSLSASTNPELGSNVSGTQTYPVV TGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGM VPGSEFSGNPYSHPQYTAYNEAWRFSNPALLSS PYYYSAAPRGSAPAAAAAAYDRH*LPRGPHQASG RQLRPPHRPRLTPPRREGGPTRRDASRPPPQPH PIPRPPQPFTSPPSKVGQDGWSRGRDPQARARR PQPRLPPLPACLDCAAP*GGFGPARPGLHQASPE ARQPPCRTRARPAGARRMFL*HTISADRSAAQP RAPASDARAPGGFAGGAGPRRLRRKRLSAGGRA RCRIPGKNSFPPVPAGLPSPSGCALSQKMEWGCG GPALGTGFGGVRSFQGWDPRIGGPSSPHRSSRT LGSSLLLLACLVPQGPAPPAARPGSQPCLAPTS ASLPPAGLPVSPPASPSPVP*RPASSSLTRSPIG RSPGTAAGPVSIDCGLGSSSSSYLMPPPPTQTL NLPVVRTGSELASELLRGGSGGLPTPLLPSPPS LLLRQELNRTTKSLHLFNMMVFAKRNKTTQKPTR LLLCGKTVCVV*RRNPVYITPPPPPRPARPRRV PVARRPCL*IRPAVCAVRVAARWGGRGGHSYTP IKAQHVLGEGGIFYVTKKNYERKEISMQNDEHGP VDSSGLFCWLFLCNSVFSLFPPCPSLPLPLSPL RFSPPLSLSLSVSVALVCLSLLFPRPLSPDLAR PPCLRRLDPRWQLQPPGSPPRGRAPRAPGGRRPG RPVPPRSCSFGSGDAPCMSPHPWIVTTRNNRNK VNKVKINKNP*TNPKRLGVLAQISLPCEPFLFE KEKEKRESFKGTRRPARLQWPERGGEGGEGAEVR PIPVLWGWPGRDPGPRPRPNLLNVPGRFWSPRP LSVRRFVLILFLLCT*EINNNNK*RIKFCMS

Sequence NM_003989

AGGCTCCAGTCTCCGGCCGAGTCTTCTCGCAGCCGCAACCCACCTGGGGCCAGCCCAGAGCTGCCAGCGCCGCTCGGCTCCCTCCCTCCCTCCCGGCCCT TCGGCCGCGGCGGCGTGCGCCTGCCTTTTCCGGGGGCGGGGGCCTGGCCCGCGCGCTCCCCTCCCGCAGGCGCCACCTCGGACATCCCCGGGATTGCTAC TTCTCTGCCAACTTCGCCAACTCGCCAGCACTTGGAGAGGCCCGGCTCCCCTCCCGGCGCCCTCTGACCGCCCCCGCCCCGCGCGCTCTCCGACCACCGC CTCTCGGATGACCAGGTTCCAGGGGAGCTGAGCGAGTCGCCTCCCCCGCCCAGCTTCAGCCCTGGCTGCAGCTGCAGCGCGAGCCATGCGCCCCCAGTGC ACCCCGGCCCGGCCCACCGCCCCGGGGCCATTCTGCTGACCGCCCAGCCCCGAGCCCCGACAGTGGCAAGTTGCGGCTACTGCAGTTGCAAGCTCCGGCC AACCCGGAGGAGCCCCAGCGGGGAGCGCAGTGCTGCGCCCCCCGCCCCCGCGCGCCCCGCAGCAGCCGGGCGTTCACTCATCCTCCCTCCCCCACCGTCC CTCCCTTTTCTCCTCAAGTCCTGAAGTTGAGTTTGAGAGGCGACACGGCGGCGGCGGCCGCGCTGCTCCCGCTCCTCTGCCTCCCCATGGATATGCACTG CAAAGCAGACCCCTTCTCCGCGATGCACCCAGGGCACGGGGGTGTGAACCAGCTCGGGGGGGTGTTTGTGAACGGCCGGCCCCTACCCGACGTGGTGAGG CAGCGCATCGTGGAGCTGGCCCACCAGGGTGTGCGGCCCTGTGACATCTCCCGGCAGCTGCGGGTCAGCCACGGCTGTGTCAGCAAAATCCTGGGCAGGT ACTACGAGACCGGCAGCATCAAGCCGGGTGTGATCGGTGGCTCCAAGCCCAAAGTGGCGACGCCCAAAGTGGTGGACAAGATTGCTGAATACAAACGACA GAACCCGACTATGTTCGCCTGGGAGATTCGAGACCGGCTCCTGGCCGAGGGCATCTGTGACAATGACACAGTGCCCAGCGTCTCTTCCATCAACAGAATC ATCCGGACCAAAGTTCAGCAGCCTTTCCACCCAACGCCGGATGGGGCTGGGACAGGAGTGACCGCCCCTGGCCACACCATTGTTCCCAGCACGGCCTCCC CTCCTGTTTCCAGCGCCTCCAATGACCCAGTGGGATCCTACTCCATCAATGGGATCCTGGGGATTCCTCGCTCCAATGGTGAGAAGAGGAAACGTGATGA AGATGTGTCTGAGGGCTCAGTCCCCAATGGAGATTCCCAGAGTGGTGTGGACAGTTTGCGGAAGCACTTGCGAGCTGACACCTTCACCCAGCAGCAGCTG GAAGCTTTGGATCGGGTCTTTGAGCGTCCTTCCTACCCTGACGTCTTCCAGGCATCAGAGCACATCAAATCAGAACAGGGGAACGAGTACTCCCTCCCAG CCCTGACCCCTGGGCTTGATGAAGTCAAGTCGAGTCTATCTGCATCCACCAACCCTGAGCTGGGCAGCAACGTGTCAGGCACACAGACATACCCAGTTGT GACTGGTCGTGACATGGCGAGCACCACTCTGCCTGGTTACCCCCCTCACGTGCCCCCCACTGGCCAGGGAAGCTACCCCACCTCCACCCTGGCAGGAATG GTGCCTGGGAGCGAGTTCTCCGGCAACCCGTACAGCCACCCCCAGTACACGGCCTACAACGAGGCTTGGAGATTCAGCAACCCCGCCTTACTAATGCCGC CCCCCGGGGCTCCGCCCCTGCCGCTGCTGCCGCTGCCTATGACCGCCACTAGTTACCGCGGGGACCACATCAAGCTTCAGGCCGACAGCTTCGGCCTCCA CATCGTCCCCGTCTGACCCCACCCCGGAGGGAGGGAGGACCGACGCGACGCGATGCCTCCCGGCCACCGCCCCAGCCTCACCCCATCCCACGACCCCCGC AACCCTTCACATCACCCCCCTCGAAGGTCGGACAGGACGGGTGGAGCCGTGGGCGGGACCCTCAGGCCCGGGCCCGCCGCCCCCAGCCCCGCCTGCCGCC CCTCCCCGCCTGCCTGGACTGCGCGGCGCCGTGAGGGGGATTCGGCCCAGCTCGTCCCGGCCTCCACCAAGCCAGCCCCGAAGCCCGCCAGCCACCCTGC CGGACTCGGGCGCGACCTGCTGGCGCGCGCCGGATGTTTCTGTGACACACAATCAGCGCGGACCGCAGCGCGGCCCAGCCCCGGGCACCCGCCTCGGACG CTCGGGCGCCAGGAGGCTTCGCTGGAGGGGCTGGGCCAAGGAGATTAAGAAGAAAACGACTTTCTGCAGGAGGAAGAGCCCGCTGCCGAATCCCTGGGAA AAATTCTTTTCCCCCAGTGCCAGCCGGACTGCCCTCGCCTTCCGGGTGTGCCCTGTCCCAGAAGATGGAATGGGGGTGTGGGGGTCCGGCTCTAGGAACG GGCTTTGGGGGCGTCAGGTCTTTCCAAGGTTGGGACCCAAGGATCGGGGGGCCCAGCAGCCCGCACCGATCGAGCCGGACTCTCGGCTCTTCACTGCTCC TCCTGGCCTGCCTAGTTCCCCAGGGCCCGGCACCTCCTGCTGCGAGACCCGGCTCTCAGCCCTGCCTTGCCCCTACCTCAGCGTCTCTTCCACCTGCTGG CCTCCCAGTTTCCCCTCCTGCCAGTCCTTCGCCTGTCCCTTGACGCCCTGCATCCTCCTCCCTGACTCGCAGCCCCATCGGACGCTCTCCCGGGACCGCC GCAGGACCAGTTTCCATAGACTGCGGACTGGGGTCTTCCTCCAGCAGTTACTTGATGCCCCCTCCCCCGACACAGACTCTCAATCTGCCGGTGGTAAGAA CCGGTTCTGAGCTGGCGTCTGAGCTGCTGCGGGGTGGAAGTGGGGGGCTGCCCACTCCACTCCTCCCATCCCCTCCCAGCCTCCTCCTCCGGCAGGAACT GAACAGAACCACAAAAAGTCTACATTTATTTAATATGATGGTCTTTGCAAAAAGGAACAAAACAACACAAAAGCCCACCAGGCTGCTGCTTTGTGGAAAG ACGGTGTGTGTCGTGTGAAGGCGAAACCCGGTGTACATAACCCCTCCCCCTCCGCCCCGCCCCGCCCGGCCCCGTAGAGTCCCTGTCGCCCGCCGGCCCT GCCTGTAGATACGCCCCGCTGTCTGTGCTGTGAGAGTCGCCGCTCGCTGGGGGGGAAGGGGGGGACACAGCTACACGCCCATTAAAGCACAGCACGTCCT GGGGGAGGGGGGCATTTTTTATGTTACAAAAAAAAATTACGAAAGAAAAGAAATCTCTATGCAAAATGACGAACATGGTCCTGTGGACTCCTCTGGCCTG TTTTGTTGGCTCTTTCTCTGTAATTCCGTGTTTTCGCTTTTTCCTCCCTGCCCCTCTCTCCCTCTGCCCCTCTCTCCTCTCCGCTTCTCTCCCCCTCTGT CTCTGTCTCTCTCCGTCTCTGTCGCTCTTGTCTGTCTGTCTCTGCTCTTTCCTCGGCCTCTCTCCCCAGACCTGGCCCGGCCGCCCTGTCTCCGCAGGCT AGATCCGAGGTGGCAGCTCCAGCCCCCGGGCTCGCCCCCTCGCGGGCGTGCCCCGCGCGCCCCGGGCGGCCGAAGGCCGGGCCGCCCCGTCCCGCCCCGT AGTTGCTCTTTCGGTAGTGGCGATGCGCCCTGCATGTCTCCTCACCCGTGGATCGTGACGACTCGAAATAACAGAAACAAAGTCAATAAAGTGAAAATAA ATAAAAATCCTTGAACAAATCCGAAAAGGCTTGGAGTCCTCGCCCAGATCTCTCTCCCCTGCGAGCCCTTTTTATTTGAGAAGGAAAAAGAGAAAAGAGA ATCGTTTAAGGGAACCCGGCGCCCAGCCAGGCTCCAGTGGCCCGAACGGGGCGGCGAGGGCGGCGAGGGCGCCGAGGTCCGGCCCATCCCAGTCCTGTGG GGCTGGCCGGGCAGAGACCCCGGACCCAGGCCCAGGCCTAACCTGCTAAATGTCCCCGGACGGTTCTGGTCTCCTCGGCCACTTTCAGTGCGTCGGTTCG TTTTGATTCTTTTTCTTTTGTGCACATAAGAAATAAATAATAATAATAAATAAAGAATAAAATTTTGTATGTCA

refseq_PAX2.F5 (forward primer)
refseq_PAX2.R5 (reverse primer)
Single exon skipping

Frame 0

RLQSPAESSRSRNPPGASPELPAPLGSLPPSRP FGRGGVRLPFPGAGAWPARSPPAGATSDIPGIAT SLPTSPTRQHLERPGSPPGAL*PPPPRALSDHR LSDDQVPGELSESPPPPSFSPGCSCSASHAPPV HPGPAHRPGAILLTAQPRAPTVASCGYCSCKLRP TRRSPSGERSAAPPAPARPAAAGRSLILPPPPS LPFLLKS*S*V*EATRRRRPRCSRSSASPWICT AKQTPSPRCTQGTGV*TSSGGCL*TAGPYPTW*G SASWSWPTRVCGPVTSPGSCGSATAVSAKSWAG TTRPAASSRV*SVAPSPKWRRPKWWTRLLNTND RTRLCSPGRFETGSWPRASVTMTQCPASLPSTES SGPKFSSLSTQRRMGLGQE*PPLATPLFPARPP LLFPAPPMTQWDPTPSMGSWGFLAPMVRRGNVM KMCLRAQSPMEIPRVVWTVCGSTCELTPSPSSSW KLWIGSLSVLPTLTSSRHQSTSNQNRGTSTPSQ P*PLGLMKSSRVYLHPPTLSWAATCQAHRHTQL *LVVTWRAPLCLVTPLTCPPLAREATPPPPWQEW CLGASSPATRTATPSTRPTTRLGDSATPPY*CR PPGLRPCRCCRCL*PPLVTAGTTSSFRPTASAS TSSPSDPTPEGGRTDATRCLPATAPASPHPTTPA TLHITPLEGRTGRVEPWAGPSGPGPPPPAPPAA PPRLPGLRGAVRGIRPSSSRPPPSQPRSPPATL PDSGATCWRAPDVSVTHNQRGPQRGPAPGTRLGR SGARRLRWRGWAKEIKKKTTFCRRKSPLPNPWE KFFSPSASRTALAFRVCPVPEDGMGVWGSGSRN GLWGRQVFPRLGPKDRGAQQPAPIEPDSRLFTAP PGLPSSPGPGTSCCETRLSALPCPYLSVSSTCW PPSFPSCQSFACPLTPCILLPDSQPHRTLSRDR RRTSFHRLRTGVFLQQLLDAPSPDTDSQSAGGKN RF*AGV*AAAGWKWGAAHSTPPIPSQPPPPAGT EQNHKKSTFI*YDGLCKKEQNNTKAHQAAALWK DGVCRVKAKPGVHNPSPSAPPRPAP*SPCRPPAL PVDTPRCLCCESRRSLGGKGGTQLHAH*STARP GGGGHFLCYKKKLRKKRNLYAK*RTWSCGLLWP VLLALSL*FRVFAFSSLPLSPSAPLSSPLLSPSV SVSLRLCRSCLSVSALSSASLPRPGPAALSPQA RSEVAAPAPGLAPSRACPARPGRPKAGPPRPAP *LLFR*WRCALHVSSPVDRDDSK*QKQSQ*SENK *KSLNKSEKAWSPRPDLSPLRALFI*EGKREKR IV*GNPAPSQAPVARTGRRGRRGRRGPAHPSPV GLAGQRPRTQAQA*PAKCPRTVLVSSATFSASVR FDSFSFVHIRNK****IKNKILYV

Frame 1

GSSLRPSLLAAATHLGPAQSCQRRSAPSLPPGP SAAAACACLFRGRGPGPRAPLPQAPPRTSPGLL LLCQLRQLASTWRGPAPLPAPSDRPRPARSPTTA SRMTRFQGS*ASRLPRPASALAAAAARAMRPQC TPARPTAPGPFC*PPSPEPRQWQVAATAVASSG QPGGAPAGSAVLRPPPPRAPQQPGVHSSSLPHRP SLFSSSPEVEFERRHGGGGRAAPAPLPPHGYAL QSRPLLRDAPRARGCEPARGGVCERPAPTRRGE AAHRGAGPPGCAAL*HLPAAAGQPRLCQQNPGQV LRDRQHQAGCDRWLQAQSGDAQSGGQDC*IQTT EPDYVRLGDSRPAPGRGHL*Q*HSAQRLFHQQN HPDQSSAAFPPNAGWGWDRSDRPWPHHCSQHGLP SCFQRLQ*PSGILLHQWDPGDSSLQW*EEET** RCV*GLSPQWRFPEWCGQFAEALAS*HLHPAAA GSFGSGL*ASFLP*RLPGIRAHQIRTGERVLPPS PDPWA**SQVESICIHQP*AGQQRVRHTDIPSC DWS*HGEHHSAWLPPSRAPHWPGKLPHLHPGRN GAWERVLRQPVQPPPVHGLQRGLEIQQPRLTNAA PRGSAPAAAAAAYDRH*LPRGPHQASGRQLRPP HRPRLTPPRREGGPTRRDASRPPPQPHPIPRPP QPFTSPPSKVGQDGWSRGRDPQARARRPQPRLPP LPACLDCAAP*GGFGPARPGLHQASPEARQPPC RTRARPAGARRMFL*HTISADRSAAQPRAPASD ARAPGGFAGGAGPRRLRRKRLSAGGRARCRIPGK NSFPPVPAGLPSPSGCALSQKMEWGCGGPALGT GFGGVRSFQGWDPRIGGPSSPHRSSRTLGSSLL LLACLVPQGPAPPAARPGSQPCLAPTSASLPPAG LPVSPPASPSPVP*RPASSSLTRSPIGRSPGTA AGPVSIDCGLGSSSSSYLMPPPPTQTLNLPVVR TGSELASELLRGGSGGLPTPLLPSPPSLLLRQEL NRTTKSLHLFNMMVFAKRNKTTQKPTRLLLCGK TVCVV*RRNPVYITPPPPPRPARPRRVPVARRP CL*IRPAVCAVRVAARWGGRGGHSYTPIKAQHVL GEGGIFYVTKKNYERKEISMQNDEHGPVDSSGL FCWLFLCNSVFSLFPPCPSLPLPLSPLRFSPPL SLSLSVSVALVCLSLLFPRPLSPDLARPPCLRRL DPRWQLQPPGSPPRGRAPRAPGGRRPGRPVPPR SCSFGSGDAPCMSPHPWIVTTRNNRNKVNKVKI NKNP*TNPKRLGVLAQISLPCEPFLFEKEKEKRE SFKGTRRPARLQWPERGGEGGEGAEVRPIPVLW GWPGRDPGPRPRPNLLNVPGRFWSPRPLSVRRF VLILFLLCT*EINNNNK*RIKFCMS

Frame 2

APVSGRVFSQPQPTWGQPRAASAARLPPSLPAL RPRRRAPAFSGGGGLARALPSRRRHLGHPRDCY FSANFANSPALGEARLPSRRPLTAPAPRALRPP PLG*PGSRGAERVASPAQLQPWLQLQREPCAPSA PRPGPPPRGHSADRPAPSPDSGKLRLLQLQAPA NPEEPQRGAQCCAPRPRAPRSSRAFTHPPSPTV PPFSPQVLKLSLRGDTAAAAALLPLLCLPMDMHC KADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQLRVSHGCVSKILGR YYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQ NPTMFAWEIRDRLLAEGICDNDTVPSVSSINRI IRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTAS PPVSSASNDPVGSYSINGILGIPRSNGEKRKRDE DVSEGSVPNGDSQSGVDSLRKHLRADTFTQQQL EALDRVFERPSYPDVFQASEHIKSEQGNEYSLP ALTPGLDEVKSSLSASTNPELGSNVSGTQTYPVV TGRDMASTTLPGYPPHVPPTGQGSYPTSTLAGM VPGSEFSGNPYSHPQYTAYNEAWRFSNPALLMP PPGAPPLPLLPLPMTATSYRGDHIKLQADSFGLH IVPV*PHPGGREDRRDAMPPGHRPSLTPSHDPR NPSHHPPRRSDRTGGAVGGTLRPGPAAPSPACR PSPPAWTARRREGDSAQLVPASTKPAPKPASHPA GLGRDLLARAGCFCDTQSARTAARPSPGHPPRT LGRQEASLEGLGQGD*EENDFLQEEEPAAESLG KILFPQCQPDCPRLPGVPCPRRWNGGVGVRL*ER ALGASGLSKVGTQGSGGPAARTDRAGLSALHCS SWPA*FPRARHLLLRDPALSPALPLPQRLFHLL ASQFPLLPVLRLSLDALHPPP*LAAPSDALPGPP QDQFP*TADWGLPPAVT*CPLPRHRLSICRW*E PVLSWRLSCCGVEVGGCPLHSSHPLPASSSGRN *TEPQKVYIYLI*WSLQKGTKQHKSPPGCCFVER RCVSCEGETRCT*PLPLRPAPPGPVESLSPAGP ACRYAPLSVL*ESPLAGGEGGDTATRPLKHSTS WGRGAFFMLQKKITKEKKSLCKMTNMVLWTPLAC FVGSFSVIPCFRFFLPAPLSLCPSLLSASLPLC LCLSPSLSLLSVCLCSFLGLSPQTWPGRPVSAG *IRGGSSSPRARPLAGVPRAPRAAEGRAAPSRPV VALSVVAMRPACLLTRGS*RLEITETKSIK*K* IKILEQIRKGLESSPRSLSPASPFYLRRKKRKE NRLREPGAQPGSSGPNGAARAARAPRSGPSQSCG AGRAETPDPGPGLTC*MSPDGSGLLGHFQCVGS F*FFFFCAHKK*IIIINKE*NFVC

Sequence NM_003990

AGGCTCCAGTCTCCGGCCGAGTCTTCTCGCAGCCGCAACCCACCTGGGGCCAGCCCAGAGCTGCCAGCGCCGCTCGGCTCCCTCCCTCCCTCCCGGCCCT TCGGCCGCGGCGGCGTGCGCCTGCCTTTTCCGGGGGCGGGGGCCTGGCCCGCGCGCTCCCCTCCCGCAGGCGCCACCTCGGACATCCCCGGGATTGCTAC TTCTCTGCCAACTTCGCCAACTCGCCAGCACTTGGAGAGGCCCGGCTCCCCTCCCGGCGCCCTCTGACCGCCCCCGCCCCGCGCGCTCTCCGACCACCGC CTCTCGGATGACCAGGTTCCAGGGGAGCTGAGCGAGTCGCCTCCCCCGCCCAGCTTCAGCCCTGGCTGCAGCTGCAGCGCGAGCCATGCGCCCCCAGTGC ACCCCGGCCCGGCCCACCGCCCCGGGGCCATTCTGCTGACCGCCCAGCCCCGAGCCCCGACAGTGGCAAGTTGCGGCTACTGCAGTTGCAAGCTCCGGCC AACCCGGAGGAGCCCCAGCGGGGAGCGCAGTGCTGCGCCCCCCGCCCCCGCGCGCCCCGCAGCAGCCGGGCGTTCACTCATCCTCCCTCCCCCACCGTCC CTCCCTTTTCTCCTCAAGTCCTGAAGTTGAGTTTGAGAGGCGACACGGCGGCGGCGGCCGCGCTGCTCCCGCTCCTCTGCCTCCCCATGGATATGCACTG CAAAGCAGACCCCTTCTCCGCGATGCACCCAGGGCACGGGGGTGTGAACCAGCTCGGGGGGGTGTTTGTGAACGGCCGGCCCCTACCCGACGTGGTGAGG CAGCGCATCGTGGAGCTGGCCCACCAGGGTGTGCGGCCCTGTGACATCTCCCGGCAGCTGCGGGTCAGCCACGGCTGTGTCAGCAAAATCCTGGGCAGGT ACTACGAGACCGGCAGCATCAAGCCGGGTGTGATCGGTGGCTCCAAGCCCAAAGTGGCGACGCCCAAAGTGGTGGACAAGATTGCTGAATACAAACGACA GAACCCGACTATGTTCGCCTGGGAGATTCGAGACCGGCTCCTGGCCGAGGGCATCTGTGACAATGACACAGTGCCCAGCGTCTCTTCCATCAACAGAATC ATCCGGACCAAAGTTCAGCAGCCTTTCCACCCAACGCCGGATGGGGCTGGGACAGGAGTGACCGCCCCTGGCCACACCATTGTTCCCAGCACGGCCTCCC CTCCTGTTTCCAGCGCCTCCAATGACCCAGTGGGATCCTACTCCATCAATGGGATCCTGGGGATTCCTCGCTCCAATGGTGAGAAGAGGAAACGTGATGA AGTTGAGGTATACACTGATCCTGCCCACATTAGAGGAGGTGGAGGTTTGCATCTGGTCTGGACTTTAAGAGATGTGTCTGAGGGCTCAGTCCCCAATGGA GATTCCCAGAGTGGTGTGGACAGTTTGCGGAAGCACTTGCGAGCTGACACCTTCACCCAGCAGCAGCTGGAAGCTTTGGATCGGGTCTTTGAGCGTCCTT CCTACCCTGACGTCTTCCAGGCATCAGAGCACATCAAATCAGAACAGGGGAACGAGTACTCCCTCCCAGCCCTGACCCCTGGGCTTGATGAAGTCAAGTC GAGTCTATCTGCATCCACCAACCCTGAGCTGGGCAGCAACGTGTCAGGCACACAGACATACCCAGTTGTGACTGGTCGTGACATGGCGAGCACCACTCTG CCTGGTTACCCCCCTCACGTGCCCCCCACTGGCCAGGGAAGCTACCCCACCTCCACCCTGGCAGGAATGGTGCCTGGGAGCGAGTTCTCCGGCAACCCGT ACAGCCACCCCCAGTACACGGCCTACAACGAGGCTTGGAGATTCAGCAACCCCGCCTTACTAATGCCGCCCCCCGGGGCTCCGCCCCTGCCGCTGCTGCC GCTGCCTATGACCGCCACTAGTTACCGCGGGGACCACATCAAGCTTCAGGCCGACAGCTTCGGCCTCCACATCGTCCCCGTCTGACCCCACCCCGGAGGG AGGGAGGACCGACGCGACGCGATGCCTCCCGGCCACCGCCCCAGCCTCACCCCATCCCACGACCCCCGCAACCCTTCACATCACCCCCCTCGAAGGTCGG ACAGGACGGGTGGAGCCGTGGGCGGGACCCTCAGGCCCGGGCCCGCCGCCCCCAGCCCCGCCTGCCGCCCCTCCCCGCCTGCCTGGACTGCGCGGCGCCG TGAGGGGGATTCGGCCCAGCTCGTCCCGGCCTCCACCAAGCCAGCCCCGAAGCCCGCCAGCCACCCTGCCGGACTCGGGCGCGACCTGCTGGCGCGCGCC GGATGTTTCTGTGACACACAATCAGCGCGGACCGCAGCGCGGCCCAGCCCCGGGCACCCGCCTCGGACGCTCGGGCGCCAGGAGGCTTCGCTGGAGGGGC TGGGCCAAGGAGATTAAGAAGAAAACGACTTTCTGCAGGAGGAAGAGCCCGCTGCCGAATCCCTGGGAAAAATTCTTTTCCCCCAGTGCCAGCCGGACTG CCCTCGCCTTCCGGGTGTGCCCTGTCCCAGAAGATGGAATGGGGGTGTGGGGGTCCGGCTCTAGGAACGGGCTTTGGGGGCGTCAGGTCTTTCCAAGGTT GGGACCCAAGGATCGGGGGGCCCAGCAGCCCGCACCGATCGAGCCGGACTCTCGGCTCTTCACTGCTCCTCCTGGCCTGCCTAGTTCCCCAGGGCCCGGC ACCTCCTGCTGCGAGACCCGGCTCTCAGCCCTGCCTTGCCCCTACCTCAGCGTCTCTTCCACCTGCTGGCCTCCCAGTTTCCCCTCCTGCCAGTCCTTCG CCTGTCCCTTGACGCCCTGCATCCTCCTCCCTGACTCGCAGCCCCATCGGACGCTCTCCCGGGACCGCCGCAGGACCAGTTTCCATAGACTGCGGACTGG GGTCTTCCTCCAGCAGTTACTTGATGCCCCCTCCCCCGACACAGACTCTCAATCTGCCGGTGGTAAGAACCGGTTCTGAGCTGGCGTCTGAGCTGCTGCG GGGTGGAAGTGGGGGGCTGCCCACTCCACTCCTCCCATCCCCTCCCAGCCTCCTCCTCCGGCAGGAACTGAACAGAACCACAAAAAGTCTACATTTATTT AATATGATGGTCTTTGCAAAAAGGAACAAAACAACACAAAAGCCCACCAGGCTGCTGCTTTGTGGAAAGACGGTGTGTGTCGTGTGAAGGCGAAACCCGG TGTACATAACCCCTCCCCCTCCGCCCCGCCCCGCCCGGCCCCGTAGAGTCCCTGTCGCCCGCCGGCCCTGCCTGTAGATACGCCCCGCTGTCTGTGCTGT GAGAGTCGCCGCTCGCTGGGGGGGAAGGGGGGGACACAGCTACACGCCCATTAAAGCACAGCACGTCCTGGGGGAGGGGGGCATTTTTTATGTTACAAAA AAAAATTACGAAAGAAAAGAAATCTCTATGCAAAATGACGAACATGGTCCTGTGGACTCCTCTGGCCTGTTTTGTTGGCTCTTTCTCTGTAATTCCGTGT TTTCGCTTTTTCCTCCCTGCCCCTCTCTCCCTCTGCCCCTCTCTCCTCTCCGCTTCTCTCCCCCTCTGTCTCTGTCTCTCTCCGTCTCTGTCGCTCTTGT CTGTCTGTCTCTGCTCTTTCCTCGGCCTCTCTCCCCAGACCTGGCCCGGCCGCCCTGTCTCCGCAGGCTAGATCCGAGGTGGCAGCTCCAGCCCCCGGGC TCGCCCCCTCGCGGGCGTGCCCCGCGCGCCCCGGGCGGCCGAAGGCCGGGCCGCCCCGTCCCGCCCCGTAGTTGCTCTTTCGGTAGTGGCGATGCGCCCT GCATGTCTCCTCACCCGTGGATCGTGACGACTCGAAATAACAGAAACAAAGTCAATAAAGTGAAAATAAATAAAAATCCTTGAACAAATCCGAAAAGGCT TGGAGTCCTCGCCCAGATCTCTCTCCCCTGCGAGCCCTTTTTATTTGAGAAGGAAAAAGAGAAAAGAGAATCGTTTAAGGGAACCCGGCGCCCAGCCAGG CTCCAGTGGCCCGAACGGGGCGGCGAGGGCGGCGAGGGCGCCGAGGTCCGGCCCATCCCAGTCCTGTGGGGCTGGCCGGGCAGAGACCCCGGACCCAGGC CCAGGCCTAACCTGCTAAATGTCCCCGGACGGTTCTGGTCTCCTCGGCCACTTTCAGTGCGTCGGTTCGTTTTGATTCTTTTTCTTTTGTGCACATAAGA AATAAATAATAATAATAAATAAAGAATAAAATTTTGTATGTCA

refseq_PAX2.F5 (forward primer)
refseq_PAX2.R5 (reverse primer)
Single exon skipping

Frame 0

RLQSPAESSRSRNPPGASPELPAPLGSLPPSRP FGRGGVRLPFPGAGAWPARSPPAGATSDIPGIAT SLPTSPTRQHLERPGSPPGAL*PPPPRALSDHR LSDDQVPGELSESPPPPSFSPGCSCSASHAPPV HPGPAHRPGAILLTAQPRAPTVASCGYCSCKLRP TRRSPSGERSAAPPAPARPAAAGRSLILPPPPS LPFLLKS*S*V*EATRRRRPRCSRSSASPWICT AKQTPSPRCTQGTGV*TSSGGCL*TAGPYPTW*G SASWSWPTRVCGPVTSPGSCGSATAVSAKSWAG TTRPAASSRV*SVAPSPKWRRPKWWTRLLNTND RTRLCSPGRFETGSWPRASVTMTQCPASLPSTES SGPKFSSLSTQRRMGLGQE*PPLATPLFPARPP LLFPAPPMTQWDPTPSMGSWGFLAPMVRRGNVM KLRYTLILPTLEEVEVCIWSGL*EMCLRAQSPME IPRVVWTVCGSTCELTPSPSSSWKLWIGSLSVL PTLTSSRHQSTSNQNRGTSTPSQP*PLGLMKSS RVYLHPPTLSWAATCQAHRHTQL*LVVTWRAPLC LVTPLTCPPLAREATPPPPWQEWCLGASSPATR TATPSTRPTTRLGDSATPPY*CRPPGLRPCRCC RCL*PPLVTAGTTSSFRPTASASTSSPSDPTPEG GRTDATRCLPATAPASPHPTTPATLHITPLEGR TGRVEPWAGPSGPGPPPPAPPAAPPRLPGLRGA VRGIRPSSSRPPPSQPRSPPATLPDSGATCWRAP DVSVTHNQRGPQRGPAPGTRLGRSGARRLRWRG WAKEIKKKTTFCRRKSPLPNPWEKFFSPSASRT ALAFRVCPVPEDGMGVWGSGSRNGLWGRQVFPRL GPKDRGAQQPAPIEPDSRLFTAPPGLPSSPGPG TSCCETRLSALPCPYLSVSSTCWPPSFPSCQSF ACPLTPCILLPDSQPHRTLSRDRRRTSFHRLRTG VFLQQLLDAPSPDTDSQSAGGKNRF*AGV*AAA GWKWGAAHSTPPIPSQPPPPAGTEQNHKKSTFI *YDGLCKKEQNNTKAHQAAALWKDGVCRVKAKPG VHNPSPSAPPRPAP*SPCRPPALPVDTPRCLCC ESRRSLGGKGGTQLHAH*STARPGGGGHFLCYK KKLRKKRNLYAK*RTWSCGLLWPVLLALSL*FRV FAFSSLPLSPSAPLSSPLLSPSVSVSLRLCRSC LSVSALSSASLPRPGPAALSPQARSEVAAPAPG LAPSRACPARPGRPKAGPPRPAP*LLFR*WRCAL HVSSPVDRDDSK*QKQSQ*SENK*KSLNKSEKA WSPRPDLSPLRALFI*EGKREKRIV*GNPAPSQ APVARTGRRGRRGRRGPAHPSPVGLAGQRPRTQA QA*PAKCPRTVLVSSATFSASVRFDSFSFVHIR NK****IKNKILYV

Frame 1

GSSLRPSLLAAATHLGPAQSCQRRSAPSLPPGP SAAAACACLFRGRGPGPRAPLPQAPPRTSPGLL LLCQLRQLASTWRGPAPLPAPSDRPRPARSPTTA SRMTRFQGS*ASRLPRPASALAAAAARAMRPQC TPARPTAPGPFC*PPSPEPRQWQVAATAVASSG QPGGAPAGSAVLRPPPPRAPQQPGVHSSSLPHRP SLFSSSPEVEFERRHGGGGRAAPAPLPPHGYAL QSRPLLRDAPRARGCEPARGGVCERPAPTRRGE AAHRGAGPPGCAAL*HLPAAAGQPRLCQQNPGQV LRDRQHQAGCDRWLQAQSGDAQSGGQDC*IQTT EPDYVRLGDSRPAPGRGHL*Q*HSAQRLFHQQN HPDQSSAAFPPNAGWGWDRSDRPWPHHCSQHGLP SCFQRLQ*PSGILLHQWDPGDSSLQW*EEET** S*GIH*SCPH*RRWRFASGLDFKRCV*GLSPQW RFPEWCGQFAEALAS*HLHPAAAGSFGSGL*ASF LP*RLPGIRAHQIRTGERVLPPSPDPWA**SQV ESICIHQP*AGQQRVRHTDIPSCDWS*HGEHHS AWLPPSRAPHWPGKLPHLHPGRNGAWERVLRQPV QPPPVHGLQRGLEIQQPRLTNAAPRGSAPAAAA AAYDRH*LPRGPHQASGRQLRPPHRPRLTPPRR EGGPTRRDASRPPPQPHPIPRPPQPFTSPPSKVG QDGWSRGRDPQARARRPQPRLPPLPACLDCAAP *GGFGPARPGLHQASPEARQPPCRTRARPAGAR RMFL*HTISADRSAAQPRAPASDARAPGGFAGGA GPRRLRRKRLSAGGRARCRIPGKNSFPPVPAGL PSPSGCALSQKMEWGCGGPALGTGFGGVRSFQG WDPRIGGPSSPHRSSRTLGSSLLLLACLVPQGPA PPAARPGSQPCLAPTSASLPPAGLPVSPPASPS PVP*RPASSSLTRSPIGRSPGTAAGPVSIDCGL GSSSSSYLMPPPPTQTLNLPVVRTGSELASELLR GGSGGLPTPLLPSPPSLLLRQELNRTTKSLHLF NMMVFAKRNKTTQKPTRLLLCGKTVCVV*RRNP VYITPPPPPRPARPRRVPVARRPCL*IRPAVCAV RVAARWGGRGGHSYTPIKAQHVLGEGGIFYVTK KNYERKEISMQNDEHGPVDSSGLFCWLFLCNSV FSLFPPCPSLPLPLSPLRFSPPLSLSLSVSVALV CLSLLFPRPLSPDLARPPCLRRLDPRWQLQPPG SPPRGRAPRAPGGRRPGRPVPPRSCSFGSGDAP CMSPHPWIVTTRNNRNKVNKVKINKNP*TNPKRL GVLAQISLPCEPFLFEKEKEKRESFKGTRRPAR LQWPERGGEGGEGAEVRPIPVLWGWPGRDPGPR PRPNLLNVPGRFWSPRPLSVRRFVLILFLLCT*E INNNNK*RIKFCMS

Frame 2

APVSGRVFSQPQPTWGQPRAASAARLPPSLPAL RPRRRAPAFSGGGGLARALPSRRRHLGHPRDCY FSANFANSPALGEARLPSRRPLTAPAPRALRPP PLG*PGSRGAERVASPAQLQPWLQLQREPCAPSA PRPGPPPRGHSADRPAPSPDSGKLRLLQLQAPA NPEEPQRGAQCCAPRPRAPRSSRAFTHPPSPTV PPFSPQVLKLSLRGDTAAAAALLPLLCLPMDMHC KADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQLRVSHGCVSKILGR YYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQ NPTMFAWEIRDRLLAEGICDNDTVPSVSSINRI IRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTAS PPVSSASNDPVGSYSINGILGIPRSNGEKRKRDE VEVYTDPAHIRGGGGLHLVWTLRDVSEGSVPNG DSQSGVDSLRKHLRADTFTQQQLEALDRVFERP SYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKS SLSASTNPELGSNVSGTQTYPVVTGRDMASTTL PGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNP YSHPQYTAYNEAWRFSNPALLMPPPGAPPLPLLP LPMTATSYRGDHIKLQADSFGLHIVPV*PHPGG REDRRDAMPPGHRPSLTPSHDPRNPSHHPPRRS DRTGGAVGGTLRPGPAAPSPACRPSPPAWTARRR EGDSAQLVPASTKPAPKPASHPAGLGRDLLARA GCFCDTQSARTAARPSPGHPPRTLGRQEASLEG LGQGD*EENDFLQEEEPAAESLGKILFPQCQPDC PRLPGVPCPRRWNGGVGVRL*ERALGASGLSKV GTQGSGGPAARTDRAGLSALHCSSWPA*FPRAR HLLLRDPALSPALPLPQRLFHLLASQFPLLPVLR LSLDALHPPP*LAAPSDALPGPPQDQFP*TADW GLPPAVT*CPLPRHRLSICRW*EPVLSWRLSCC GVEVGGCPLHSSHPLPASSSGRN*TEPQKVYIYL I*WSLQKGTKQHKSPPGCCFVERRCVSCEGETR CT*PLPLRPAPPGPVESLSPAGPACRYAPLSVL *ESPLAGGEGGDTATRPLKHSTSWGRGAFFMLQK KITKEKKSLCKMTNMVLWTPLACFVGSFSVIPC FRFFLPAPLSLCPSLLSASLPLCLCLSPSLSLL SVCLCSFLGLSPQTWPGRPVSAG*IRGGSSSPRA RPLAGVPRAPRAAEGRAAPSRPVVALSVVAMRP ACLLTRGS*RLEITETKSIK*K*IKILEQIRKG LESSPRSLSPASPFYLRRKKRKENRLREPGAQPG SSGPNGAARAARAPRSGPSQSCGAGRAETPDPG PGLTC*MSPDGSGLLGHFQCVGSF*FFFFCAHK K*IIIINKE*NFVC

Sequence NM_003987

AGGCTCCAGTCTCCGGCCGAGTCTTCTCGCAGCCGCAACCCACCTGGGGCCAGCCCAGAGCTGCCAGCGCCGCTCGGCTCCCTCCCTCCCTCCCGGCCCT TCGGCCGCGGCGGCGTGCGCCTGCCTTTTCCGGGGGCGGGGGCCTGGCCCGCGCGCTCCCCTCCCGCAGGCGCCACCTCGGACATCCCCGGGATTGCTAC TTCTCTGCCAACTTCGCCAACTCGCCAGCACTTGGAGAGGCCCGGCTCCCCTCCCGGCGCCCTCTGACCGCCCCCGCCCCGCGCGCTCTCCGACCACCGC CTCTCGGATGACCAGGTTCCAGGGGAGCTGAGCGAGTCGCCTCCCCCGCCCAGCTTCAGCCCTGGCTGCAGCTGCAGCGCGAGCCATGCGCCCCCAGTGC ACCCCGGCCCGGCCCACCGCCCCGGGGCCATTCTGCTGACCGCCCAGCCCCGAGCCCCGACAGTGGCAAGTTGCGGCTACTGCAGTTGCAAGCTCCGGCC AACCCGGAGGAGCCCCAGCGGGGAGCGCAGTGCTGCGCCCCCCGCCCCCGCGCGCCCCGCAGCAGCCGGGCGTTCACTCATCCTCCCTCCCCCACCGTCC CTCCCTTTTCTCCTCAAGTCCTGAAGTTGAGTTTGAGAGGCGACACGGCGGCGGCGGCCGCGCTGCTCCCGCTCCTCTGCCTCCCCATGGATATGCACTG CAAAGCAGACCCCTTCTCCGCGATGCACCCAGGGCACGGGGGTGTGAACCAGCTCGGGGGGGTGTTTGTGAACGGCCGGCCCCTACCCGACGTGGTGAGG CAGCGCATCGTGGAGCTGGCCCACCAGGGTGTGCGGCCCTGTGACATCTCCCGGCAGCTGCGGGTCAGCCACGGCTGTGTCAGCAAAATCCTGGGCAGGT ACTACGAGACCGGCAGCATCAAGCCGGGTGTGATCGGTGGCTCCAAGCCCAAAGTGGCGACGCCCAAAGTGGTGGACAAGATTGCTGAATACAAACGACA GAACCCGACTATGTTCGCCTGGGAGATTCGAGACCGGCTCCTGGCCGAGGGCATCTGTGACAATGACACAGTGCCCAGCGTCTCTTCCATCAACAGAATC ATCCGGACCAAAGTTCAGCAGCCTTTCCACCCAACGCCGGATGGGGCTGGGACAGGAGTGACCGCCCCTGGCCACACCATTGTTCCCAGCACGGCCTCCC CTCCTGTTTCCAGCGCCTCCAATGACCCAGTGGGATCCTACTCCATCAATGGGATCCTGGGGATTCCTCGCTCCAATGGTGAGAAGAGGAAACGTGATGA AGTTGAGGTATACACTGATCCTGCCCACATTAGAGGAGGTGGAGGTTTGCATCTGGTCTGGACTTTAAGAGATGTGTCTGAGGGCTCAGTCCCCAATGGA GATTCCCAGAGTGGTGTGGACAGTTTGCGGAAGCACTTGCGAGCTGACACCTTCACCCAGCAGCAGCTGGAAGCTTTGGATCGGGTCTTTGAGCGTCCTT CCTACCCTGACGTCTTCCAGGCATCAGAGCACATCAAATCAGAACAGGGGAACGAGTACTCCCTCCCAGCCCTGACCCCTGGGCTTGATGAAGTCAAGTC GAGTCTATCTGCATCCACCAACCCTGAGCTGGGCAGCAACGTGTCAGGCACACAGACATACCCAGTTGTGACTGGTCGTGACATGGCGAGCACCACTCTG CCTGGTTACCCCCCTCACGTGCCCCCCACTGGCCAGGGAAGCTACCCCACCTCCACCCTGGCAGGAATGGTGCCTGGGAGCGAGTTCTCCGGCAACCCGT ACAGCCACCCCCAGTACACGGCCTACAACGAGGCTTGGAGATTCAGCAACCCCGCCTTACTAAGTTCCCCTTATTATTATAGTGCCGCCCCCCGGGGCTC CGCCCCTGCCGCTGCTGCCGCTGCCTATGACCGCCACTAGTTACCGCGGGGACCACATCAAGCTTCAGGCCGACAGCTTCGGCCTCCACATCGTCCCCGT CTGACCCCACCCCGGAGGGAGGGAGGACCGACGCGACGCGATGCCTCCCGGCCACCGCCCCAGCCTCACCCCATCCCACGACCCCCGCAACCCTTCACAT CACCCCCCTCGAAGGTCGGACAGGACGGGTGGAGCCGTGGGCGGGACCCTCAGGCCCGGGCCCGCCGCCCCCAGCCCCGCCTGCCGCCCCTCCCCGCCTG CCTGGACTGCGCGGCGCCGTGAGGGGGATTCGGCCCAGCTCGTCCCGGCCTCCACCAAGCCAGCCCCGAAGCCCGCCAGCCACCCTGCCGGACTCGGGCG CGACCTGCTGGCGCGCGCCGGATGTTTCTGTGACACACAATCAGCGCGGACCGCAGCGCGGCCCAGCCCCGGGCACCCGCCTCGGACGCTCGGGCGCCAG GAGGCTTCGCTGGAGGGGCTGGGCCAAGGAGATTAAGAAGAAAACGACTTTCTGCAGGAGGAAGAGCCCGCTGCCGAATCCCTGGGAAAAATTCTTTTCC CCCAGTGCCAGCCGGACTGCCCTCGCCTTCCGGGTGTGCCCTGTCCCAGAAGATGGAATGGGGGTGTGGGGGTCCGGCTCTAGGAACGGGCTTTGGGGGC GTCAGGTCTTTCCAAGGTTGGGACCCAAGGATCGGGGGGCCCAGCAGCCCGCACCGATCGAGCCGGACTCTCGGCTCTTCACTGCTCCTCCTGGCCTGCC TAGTTCCCCAGGGCCCGGCACCTCCTGCTGCGAGACCCGGCTCTCAGCCCTGCCTTGCCCCTACCTCAGCGTCTCTTCCACCTGCTGGCCTCCCAGTTTC CCCTCCTGCCAGTCCTTCGCCTGTCCCTTGACGCCCTGCATCCTCCTCCCTGACTCGCAGCCCCATCGGACGCTCTCCCGGGACCGCCGCAGGACCAGTT TCCATAGACTGCGGACTGGGGTCTTCCTCCAGCAGTTACTTGATGCCCCCTCCCCCGACACAGACTCTCAATCTGCCGGTGGTAAGAACCGGTTCTGAGC TGGCGTCTGAGCTGCTGCGGGGTGGAAGTGGGGGGCTGCCCACTCCACTCCTCCCATCCCCTCCCAGCCTCCTCCTCCGGCAGGAACTGAACAGAACCAC AAAAAGTCTACATTTATTTAATATGATGGTCTTTGCAAAAAGGAACAAAACAACACAAAAGCCCACCAGGCTGCTGCTTTGTGGAAAGACGGTGTGTGTC GTGTGAAGGCGAAACCCGGTGTACATAACCCCTCCCCCTCCGCCCCGCCCCGCCCGGCCCCGTAGAGTCCCTGTCGCCCGCCGGCCCTGCCTGTAGATAC GCCCCGCTGTCTGTGCTGTGAGAGTCGCCGCTCGCTGGGGGGGAAGGGGGGGACACAGCTACACGCCCATTAAAGCACAGCACGTCCTGGGGGAGGGGGG CATTTTTTATGTTACAAAAAAAAATTACGAAAGAAAAGAAATCTCTATGCAAAATGACGAACATGGTCCTGTGGACTCCTCTGGCCTGTTTTGTTGGCTC TTTCTCTGTAATTCCGTGTTTTCGCTTTTTCCTCCCTGCCCCTCTCTCCCTCTGCCCCTCTCTCCTCTCCGCTTCTCTCCCCCTCTGTCTCTGTCTCTCT CCGTCTCTGTCGCTCTTGTCTGTCTGTCTCTGCTCTTTCCTCGGCCTCTCTCCCCAGACCTGGCCCGGCCGCCCTGTCTCCGCAGGCTAGATCCGAGGTG GCAGCTCCAGCCCCCGGGCTCGCCCCCTCGCGGGCGTGCCCCGCGCGCCCCGGGCGGCCGAAGGCCGGGCCGCCCCGTCCCGCCCCGTAGTTGCTCTTTC GGTAGTGGCGATGCGCCCTGCATGTCTCCTCACCCGTGGATCGTGACGACTCGAAATAACAGAAACAAAGTCAATAAAGTGAAAATAAATAAAAATCCTT GAACAAATCCGAAAAGGCTTGGAGTCCTCGCCCAGATCTCTCTCCCCTGCGAGCCCTTTTTATTTGAGAAGGAAAAAGAGAAAAGAGAATCGTTTAAGGG AACCCGGCGCCCAGCCAGGCTCCAGTGGCCCGAACGGGGCGGCGAGGGCGGCGAGGGCGCCGAGGTCCGGCCCATCCCAGTCCTGTGGGGCTGGCCGGGC AGAGACCCCGGACCCAGGCCCAGGCCTAACCTGCTAAATGTCCCCGGACGGTTCTGGTCTCCTCGGCCACTTTCAGTGCGTCGGTTCGTTTTGATTCTTT TTCTTTTGTGCACATAAGAAATAAATAATAATAATAAATAAAGAATAAAATTTTGTATGTCA

refseq_PAX2.F5 (forward primer)
refseq_PAX2.R5 (reverse primer)
Single exon skipping

Frame 0

RLQSPAESSRSRNPPGASPELPAPLGSLPPSRP FGRGGVRLPFPGAGAWPARSPPAGATSDIPGIAT SLPTSPTRQHLERPGSPPGAL*PPPPRALSDHR LSDDQVPGELSESPPPPSFSPGCSCSASHAPPV HPGPAHRPGAILLTAQPRAPTVASCGYCSCKLRP TRRSPSGERSAAPPAPARPAAAGRSLILPPPPS LPFLLKS*S*V*EATRRRRPRCSRSSASPWICT AKQTPSPRCTQGTGV*TSSGGCL*TAGPYPTW*G SASWSWPTRVCGPVTSPGSCGSATAVSAKSWAG TTRPAASSRV*SVAPSPKWRRPKWWTRLLNTND RTRLCSPGRFETGSWPRASVTMTQCPASLPSTES SGPKFSSLSTQRRMGLGQE*PPLATPLFPARPP LLFPAPPMTQWDPTPSMGSWGFLAPMVRRGNVM KLRYTLILPTLEEVEVCIWSGL*EMCLRAQSPME IPRVVWTVCGSTCELTPSPSSSWKLWIGSLSVL PTLTSSRHQSTSNQNRGTSTPSQP*PLGLMKSS RVYLHPPTLSWAATCQAHRHTQL*LVVTWRAPLC LVTPLTCPPLAREATPPPPWQEWCLGASSPATR TATPSTRPTTRLGDSATPPY*VPLIIIVPPPGA PPLPLLPLPMTATSYRGDHIKLQADSFGLHIVPV *PHPGGREDRRDAMPPGHRPSLTPSHDPRNPSH HPPRRSDRTGGAVGGTLRPGPAAPSPACRPSPP AWTARRREGDSAQLVPASTKPAPKPASHPAGLGR DLLARAGCFCDTQSARTAARPSPGHPPRTLGRQ EASLEGLGQGD*EENDFLQEEEPAAESLGKILF PQCQPDCPRLPGVPCPRRWNGGVGVRL*ERALGA SGLSKVGTQGSGGPAARTDRAGLSALHCSSWPA *FPRARHLLLRDPALSPALPLPQRLFHLLASQF PLLPVLRLSLDALHPPP*LAAPSDALPGPPQDQF P*TADWGLPPAVT*CPLPRHRLSICRW*EPVLS WRLSCCGVEVGGCPLHSSHPLPASSSGRN*TEP QKVYIYLI*WSLQKGTKQHKSPPGCCFVERRCVS CEGETRCT*PLPLRPAPPGPVESLSPAGPACRY APLSVL*ESPLAGGEGGDTATRPLKHSTSWGRG AFFMLQKKITKEKKSLCKMTNMVLWTPLACFVGS FSVIPCFRFFLPAPLSLCPSLLSASLPLCLCLS PSLSLLSVCLCSFLGLSPQTWPGRPVSAG*IRG GSSSPRARPLAGVPRAPRAAEGRAAPSRPVVALS VVAMRPACLLTRGS*RLEITETKSIK*K*IKIL EQIRKGLESSPRSLSPASPFYLRRKKRKENRLR EPGAQPGSSGPNGAARAARAPRSGPSQSCGAGRA ETPDPGPGLTC*MSPDGSGLLGHFQCVGSF*FF FFCAHKK*IIIINKE*NFVC

Frame 1

GSSLRPSLLAAATHLGPAQSCQRRSAPSLPPGP SAAAACACLFRGRGPGPRAPLPQAPPRTSPGLL LLCQLRQLASTWRGPAPLPAPSDRPRPARSPTTA SRMTRFQGS*ASRLPRPASALAAAAARAMRPQC TPARPTAPGPFC*PPSPEPRQWQVAATAVASSG QPGGAPAGSAVLRPPPPRAPQQPGVHSSSLPHRP SLFSSSPEVEFERRHGGGGRAAPAPLPPHGYAL QSRPLLRDAPRARGCEPARGGVCERPAPTRRGE AAHRGAGPPGCAAL*HLPAAAGQPRLCQQNPGQV LRDRQHQAGCDRWLQAQSGDAQSGGQDC*IQTT EPDYVRLGDSRPAPGRGHL*Q*HSAQRLFHQQN HPDQSSAAFPPNAGWGWDRSDRPWPHHCSQHGLP SCFQRLQ*PSGILLHQWDPGDSSLQW*EEET** S*GIH*SCPH*RRWRFASGLDFKRCV*GLSPQW RFPEWCGQFAEALAS*HLHPAAAGSFGSGL*ASF LP*RLPGIRAHQIRTGERVLPPSPDPWA**SQV ESICIHQP*AGQQRVRHTDIPSCDWS*HGEHHS AWLPPSRAPHWPGKLPHLHPGRNGAWERVLRQPV QPPPVHGLQRGLEIQQPRLTKFPLLL*CRPPGL RPCRCCRCL*PPLVTAGTTSSFRPTASASTSSP SDPTPEGGRTDATRCLPATAPASPHPTTPATLHI TPLEGRTGRVEPWAGPSGPGPPPPAPPAAPPRL PGLRGAVRGIRPSSSRPPPSQPRSPPATLPDSG ATCWRAPDVSVTHNQRGPQRGPAPGTRLGRSGAR RLRWRGWAKEIKKKTTFCRRKSPLPNPWEKFFS PSASRTALAFRVCPVPEDGMGVWGSGSRNGLWG RQVFPRLGPKDRGAQQPAPIEPDSRLFTAPPGLP SSPGPGTSCCETRLSALPCPYLSVSSTCWPPSF PSCQSFACPLTPCILLPDSQPHRTLSRDRRRTS FHRLRTGVFLQQLLDAPSPDTDSQSAGGKNRF*A GV*AAAGWKWGAAHSTPPIPSQPPPPAGTEQNH KKSTFI*YDGLCKKEQNNTKAHQAAALWKDGVC RVKAKPGVHNPSPSAPPRPAP*SPCRPPALPVDT PRCLCCESRRSLGGKGGTQLHAH*STARPGGGG HFLCYKKKLRKKRNLYAK*RTWSCGLLWPVLLA LSL*FRVFAFSSLPLSPSAPLSSPLLSPSVSVSL RLCRSCLSVSALSSASLPRPGPAALSPQARSEV AAPAPGLAPSRACPARPGRPKAGPPRPAP*LLF R*WRCALHVSSPVDRDDSK*QKQSQ*SENK*KSL NKSEKAWSPRPDLSPLRALFI*EGKREKRIV*G NPAPSQAPVARTGRRGRRGRRGPAHPSPVGLAG QRPRTQAQA*PAKCPRTVLVSSATFSASVRFDSF SFVHIRNK****IKNKILYV

Frame 2

APVSGRVFSQPQPTWGQPRAASAARLPPSLPAL RPRRRAPAFSGGGGLARALPSRRRHLGHPRDCY FSANFANSPALGEARLPSRRPLTAPAPRALRPP PLG*PGSRGAERVASPAQLQPWLQLQREPCAPSA PRPGPPPRGHSADRPAPSPDSGKLRLLQLQAPA NPEEPQRGAQCCAPRPRAPRSSRAFTHPPSPTV PPFSPQVLKLSLRGDTAAAAALLPLLCLPMDMHC KADPFSAMHPGHGGVNQLGGVFVNGRPLPDVVR QRIVELAHQGVRPCDISRQLRVSHGCVSKILGR YYETGSIKPGVIGGSKPKVATPKVVDKIAEYKRQ NPTMFAWEIRDRLLAEGICDNDTVPSVSSINRI IRTKVQQPFHPTPDGAGTGVTAPGHTIVPSTAS PPVSSASNDPVGSYSINGILGIPRSNGEKRKRDE VEVYTDPAHIRGGGGLHLVWTLRDVSEGSVPNG DSQSGVDSLRKHLRADTFTQQQLEALDRVFERP SYPDVFQASEHIKSEQGNEYSLPALTPGLDEVKS SLSASTNPELGSNVSGTQTYPVVTGRDMASTTL PGYPPHVPPTGQGSYPTSTLAGMVPGSEFSGNP YSHPQYTAYNEAWRFSNPALLSSPYYYSAAPRGS APAAAAAAYDRH*LPRGPHQASGRQLRPPHRPR LTPPRREGGPTRRDASRPPPQPHPIPRPPQPFT SPPSKVGQDGWSRGRDPQARARRPQPRLPPLPAC LDCAAP*GGFGPARPGLHQASPEARQPPCRTRA RPAGARRMFL*HTISADRSAAQPRAPASDARAP GGFAGGAGPRRLRRKRLSAGGRARCRIPGKNSFP PVPAGLPSPSGCALSQKMEWGCGGPALGTGFGG VRSFQGWDPRIGGPSSPHRSSRTLGSSLLLLAC LVPQGPAPPAARPGSQPCLAPTSASLPPAGLPVS PPASPSPVP*RPASSSLTRSPIGRSPGTAAGPV SIDCGLGSSSSSYLMPPPPTQTLNLPVVRTGSE LASELLRGGSGGLPTPLLPSPPSLLLRQELNRTT KSLHLFNMMVFAKRNKTTQKPTRLLLCGKTVCV V*RRNPVYITPPPPPRPARPRRVPVARRPCL*I RPAVCAVRVAARWGGRGGHSYTPIKAQHVLGEGG IFYVTKKNYERKEISMQNDEHGPVDSSGLFCWL FLCNSVFSLFPPCPSLPLPLSPLRFSPPLSLSL SVSVALVCLSLLFPRPLSPDLARPPCLRRLDPRW QLQPPGSPPRGRAPRAPGGRRPGRPVPPRSCSF GSGDAPCMSPHPWIVTTRNNRNKVNKVKINKNP *TNPKRLGVLAQISLPCEPFLFEKEKEKRESFKG TRRPARLQWPERGGEGGEGAEVRPIPVLWGWPG RDPGPRPRPNLLNVPGRFWSPRPLSVRRFVLIL FLLCT*EINNNNK*RIKFCMS