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Sequences

Sequence NM_001040007

CACAGCAGCCCCCGCGCCCGCCGTGCCGCCGCCGGGACGTGGGGCCCTTGGGCCGTCGGGCCGCCTGGGGAGCGCCAGCCCGGATCCGGCTGCCCAGATG CGGGCGCCACTCTGCCTGCTCCTGCTCGTCGCCCACGCCGTGGACATGCTCGCCCTGAACCGAAGGAAGAAGCAAGTGGGCACTGGCCTGGGGGGCAACT GCACAGGCTGTATCATCTGCTCAGAGGAGAACGGCTGTTCCACCTGCCAGCAGAGGCTCTTCCTGTTCATCCGCCGGGAAGGCATCCGCCAGTACGGCAA GTGCCTGCACGACTGTCCCCCTGGGTACTTCGGCATCCGCGGCCAGGAGGTCAACAGGTGCAAAAAATGTGGGGCCACTTGTGAGAGCTGCTTCAGCCAG GACTTCTGCATCCGGTGCAAGAGGCAGTTTTACTTGTACAAGGGGAAGTGTCTGCCCACCTGCCCGCCGGGCACTTTGGCCCACCAGAACACACGGGAGT GCCAGGAGAGGAGCCCCGGCCAGAAGAAGGGCAGGAAGGACCGGCGCCCACGCAAGGACAGGAAGCTGGACCGCAGGCTGGACGTGAGGCCGCGCCAGCC CGGCCTGCAGCCCTGACCGCCGGCTCTCCCGACTCTCTGGTCCTAGTCCTCGGCCCCTGCACACCTCCTCCTGCTCCTTCTCCTCCTCTCCTCTTACTCT TTCTCCTCTGTCTTCTCCATTTGTCCTCTCTTTCTTTCCACCCTTCTATCATTTTTCTGTCAGTCTACCTTCCCTTTCTTTTTCTTTTTTATTTCCTTTA TTTCTTCCACCTCCATTCTCCTCTCCTTTCTCCCTCCCTCCTTCCCTTCCTTCCTCTTCTTTCTCACTTATCTTTTATCTTTCCTTTTCTTTCTTCCTGT GTTTCTTCCTGTCCTTCACCGCATCCTTCTCTCTCTCCCTCCTCTTGTCTCCCTCTCACACACACTTTAAGAGGGACCATGAGCCTGTGCCCTCCCCTGC AGCTTTCTCTATCTACAACTTAAAGAAAGCAAACATCTTTTCCCAGGCCTTTCCCTGACCCCATCTTTGCAGAGAAAGGGTTTCCAGAGGGCAAAGCTGG GACACAGCACAGGTGAATCCTGAAGGCCCTGCTTCTGCTCTGGGGGAGGCTCCAGGACCCTGAGCTGTGAGCACCTGGTTCTCTGGACAGTCCCCAGAGG CCATTTCCACAGCCTTCAGCCACCAGCCACCCCGAGGAGCTGGCTGGACAAGGCTCCAGGGCTTCCAGAGGCCTGGCTTGGACACCTCCCCCAGCTGGCC GTGGAGGGTCACAACCTGGCCTCTGGGTGGGCAGCCAGCCCTGGAGGGCATCCTCTGCAAGCTGCCTGCCACCCTCATCGGCACTCCCCCACAGGCCTCC CTCTCATGGGTTCCATGCCCCTTTTTCCCAAGCCGGATCAGGTGAGCTGTCACTGCTGGGGGATCCACCTGCCCAGCCCAGAAGAGGCCACTGAAACGGA AAGGAAAGCTGAGATTATCCAGCAGCTCTGTTCCCCACCTCAGCGCTTCCTGCCCATGTGGGGAAACAGGTCTGAGAAGGAAGGGGCTTGCCCAGGGTCA CACAGGAAGCCTTCAGGCTCTGCTTCTGCCTGATGGCTCTGCTCAGCACATTCACGGTGGAGAGGAGAATTTGGGGGTCACTTGAGGGGGGAAATGTAGG GAATTGTGGGTGGGGAGCAAGGGAAGATCCGTGCACTCGTCCACACCCACCACCACACTCGCTGACACCCACCCCCACACGCTGACACCCACCCCCACAC TTGCCCACACCCATCACCGCACTCGCCCACACCCACCACCACACTGCCCCACACCCACCACCACACTCCCCCACACCCACCACCACACTCGCCCACACCC ACCACCAGTGACTTGAGCATCTGTGCTTCGCTGTGACGCCCCTCGCCCTAGGCAGGAACGACGCTGGGAGGAGTCTCCAGGTCAGACCCAGCTTGGAAGC AAGTCTGTCCTCACTGCCTATCCTTCTGCCATCATAACACCCCCTTCCTGCTCTGCTCCCCGGAATCCTCAGAAACGGGATTTGTATTTGCCGTGACTGG TTGGCCTGAACACGTAGGGCTCCGTGACTGGGACAGGAATGGGCAGGAGAAGCAAGAGTCGGAGCTCCAAGGGGCCCAGGGGTGGCCTGGGGAAGGAAGA TGGTCAGCAGGCTGGGGGAGAGGCTCTAGGTGATGAAATATTACATTCCCGACCCCAAGAGAGCACCCACCCTCAGACCTGCCCTCCACCTGGCAGCTGG GGAGCCCTGGCCTGAACCCCCCCCTCCCAGCAGGCCCACCCTCTCTCTGACTTCCCTGCTCTCACCTCCCCGAGAACAGCTAGAGCCCCCTCCTCCGCCT GGCCAGGCCACCAGCTTCTCTTCTGCAAACGTTTGTGCCTCTGAAATGCTCCGTTGTTATTGTTTCAAGACCCTAACTTTTTTTTAAAACTTTCTTAATA AAGGGAAAAGAAACTTGTAAA

refseq_RSPO4.F1 (forward primer)
refseq_RSPO4.R1 (reverse primer)
Single exon skipping

Frame 0

HSSPRARRAAAGTWGPWAVGPPGERQPGSGCPD AGATLPAPARRPRRGHARPEPKEEASGHWPGGQL HRLYHLLRGERLFHLPAEALPVHPPGRHPPVRQ VPARLSPWVLRHPRPGGQQVQKMWGHL*ELLQP GLLHPVQEAVLLVQGEVSAHLPAGHFGPPEHTGV PGEEPRPEEGQEGPAPTQGQEAGPQAGREAAPA RPAALTAGSPDSLVLVLGPCTPPPAPSPPLLLL FLLCLLHLSSLSFHPSIIFLSVYLPFLFLFYFLY FFHLHSPLLSPSLLPFLPLLSHLSFIFPFLSSC VSSCPSPHPSLSPSSCLPLTHTLRGTMSLCPPL QLSLSTT*RKQTSFPRPFPDPIFAEKGFPEGKAG TQHR*ILKALLLLWGRLQDPEL*APGSLDSPQR PFPQPSATSHPEELAGQGSRASRGLAWTPPPAG RGGSQPGLWVGSQPWRASSASCLPPSSALPHRPP SHGFHAPFSQAGSGELSLLGDPPAQPRRGH*NG KES*DYPAALFPTSALPAHVGKQV*EGRGLPRV TQEAFRLCFCLMALLSTFTVERRIWGSLEGGNVG NCGWGAREDPCTRPHPPPHSLTPTPTR*HPPPH LPTPITALAHTHHHTAPHPPPHSPTPTTTLAHT HHQ*LEHLCFAVTPLALGRNDAGRSLQVRPSLEA SLSSLPILLPS*HPLPALLPGILRNGICICRDW LA*TRRAP*LGQEWAGEARVGAPRGPGVAWGRK MVSRLGERL*VMKYYIPDPKRAPTLRPALHLAAG EPWPEPPPPSRPTLSLTSLLSPPREQLEPPPPP GQATSFSSANVCASEMLRCYCFKTLTFF*NFLN KGKRNL*

Frame 1

TAAPAPAVPPPGRGALGPSGRLGSASPDPAAQM RAPLCLLLLVAHAVDMLALNRRKKQVGTGLGGN CTGCIICSEENGCSTCQQRLFLFIRREGIRQYGK CLHDCPPGYFGIRGQEVNRCKKCGATCESCFSQ DFCIRCKRQFYLYKGKCLPTCPPGTLAHQNTRE CQERSPGQKKGRKDRRPRKDRKLDRRLDVRPRQP GLQP*PPALPTLWS*SSAPAHLLLLLLLLSSYS FSSVFSICPLFLSTLLSFFCQSTFPFFFFFISF ISSTSILLSFLPPSFPSFLFFLTYLLSFLFFLPV FLPVLHRILLSLPPLVSLSHTL*EGP*ACALPC SFLYLQLKESKHLFPGLSLTPSLQRKGFQRAKL GHSTGES*RPCFCSGGGSRTLSCEHLVLWTVPRG HFHSLQPPATPRSWLDKAPGLPEAWLGHLPQLA VEGHNLASGWAASPGGHPLQAACHPHRHSPTGL PLMGSMPLFPKPDQVSCHCWGIHLPSPEEATETE RKAEIIQQLCSPPQRFLPMWGNRSEKEGACPGS HRKPSGSASA*WLCSAHSRWRGEFGGHLRGEM* GIVGGEQGKIRALVHTHHHTR*HPPPHADTHPHT CPHPSPHSPTPTTTLPHTHHHTPPHPPPHSPTP TTSDLSICASL*RPSP*AGTTLGGVSRSDPAWK QVCPHCLSFCHHNTPFLLCSPESSETGFVFAVTG WPEHVGLRDWDRNGQEKQESELQGAQGWPGEGR WSAGWGRGSR**NITFPTPREHPPSDLPSTWQL GSPGLNPPLPAGPPSL*LPCSHLPENS*SPLLRL ARPPASLLQTFVPLKCSVVIVSRP*LFFKTFLI KGKETCK

Frame 2

QQPPRPPCRRRDVGPLGRRAAWGAPARIRLPRC GRHSACSCSSPTPWTCSP*TEGRSKWALAWGAT AQAVSSAQRRTAVPPASRGSSCSSAGKASASTA SACTTVPLGTSASAARRSTGAKNVGPLVRAASAR TSASGARGSFTCTRGSVCPPARRALWPTRTHGS ARRGAPARRRAGRTGAHARTGSWTAGWT*GRAS PACSPDRRLSRLSGPSPRPLHTSSCSFSSSPLTL SPLSSPFVLSFFPPFYHFSVSLPSLSFSFLFPL FLPPPFSSPFSLPPSLPSSSFSLIFYLSFSFFL CFFLSFTASFSLSLLLSPSHTHFKRDHEPVPSPA AFSIYNLKKANIFSQAFP*PHLCRERVSRGQSW DTAQVNPEGPASALGEAPGP*AVSTWFSGQSPE AISTAFSHQPPRGAGWTRLQGFQRPGLDTSPSWP WRVTTWPLGGQPALEGILCKLPATLIGTPPQAS LSWVPCPFFPSRIR*AVTAGGSTCPAQKRPLKR KGKLRLSSSSVPHLSASCPCGETGLRRKGLAQGH TGSLQALLLPDGSAQHIHGGEENLGVT*GGKCR ELWVGSKGRSVHSSTPTTTLADTHPHTLTPTPT LAHTHHRTRPHPPPHCPTPTTTLPHTHHHTRPHP PPVT*ASVLRCDAPRPRQERRWEESPGQTQLGS KSVLTAYPSAIITPPSCSAPRNPQKRDLYLP*L VGLNT*GSVTGTGMGRRSKSRSSKGPRGGLGKED GQQAGGEALGDEILHSRPQESTHPQTCPPPGSW GALA*TPPSQQAHPLSDFPALTSPRTARAPSSA WPGHQLLFCKRLCL*NAPLLLFQDPNFFLKLS** REKKLV

Sequence NM_001029871

CACAGCAGCCCCCGCGCCCGCCGTGCCGCCGCCGGGACGTGGGGCCCTTGGGCCGTCGGGCCGCCTGGGGAGCGCCAGCCCGGATCCGGCTGCCCAGATG CGGGCGCCACTCTGCCTGCTCCTGCTCGTCGCCCACGCCGTGGACATGCTCGCCCTGAACCGAAGGAAGAAGCAAGTGGGCACTGGCCTGGGGGGCAACT GCACAGGCTGTATCATCTGCTCAGAGGAGAACGGCTGTTCCACCTGCCAGCAGAGGCTCTTCCTGTTCATCCGCCGGGAAGGCATCCGCCAGTACGGCAA GTGCCTGCACGACTGTCCCCCTGGGTACTTCGGCATCCGCGGCCAGGAGGTCAACAGGTGCAAAAAATGTGGGGCCACTTGTGAGAGCTGCTTCAGCCAG GACTTCTGCATCCGGTGCAAGAGGCAGTTTTACTTGTACAAGGGGAAGTGTCTGCCCACCTGCCCGCCGGGCACTTTGGCCCACCAGAACACACGGGAGT GCCAGGGGGAGTGTGAACTGGGTCCCTGGGGCGGCTGGAGCCCCTGCACACACAATGGAAAGACCTGCGGCTCGGCTTGGGGCCTGGAGAGCCGGGTACG AGAGGCTGGCCGGGCTGGGCATGAGGAGGCAGCCACCTGCCAGGTGCTTTCTGAGTCAAGGAAATGTCCCATCCAGAGGCCCTGCCCAGGAGAGAGGAGC CCCGGCCAGAAGAAGGGCAGGAAGGACCGGCGCCCACGCAAGGACAGGAAGCTGGACCGCAGGCTGGACGTGAGGCCGCGCCAGCCCGGCCTGCAGCCCT GACCGCCGGCTCTCCCGACTCTCTGGTCCTAGTCCTCGGCCCCTGCACACCTCCTCCTGCTCCTTCTCCTCCTCTCCTCTTACTCTTTCTCCTCTGTCTT CTCCATTTGTCCTCTCTTTCTTTCCACCCTTCTATCATTTTTCTGTCAGTCTACCTTCCCTTTCTTTTTCTTTTTTATTTCCTTTATTTCTTCCACCTCC ATTCTCCTCTCCTTTCTCCCTCCCTCCTTCCCTTCCTTCCTCTTCTTTCTCACTTATCTTTTATCTTTCCTTTTCTTTCTTCCTGTGTTTCTTCCTGTCC TTCACCGCATCCTTCTCTCTCTCCCTCCTCTTGTCTCCCTCTCACACACACTTTAAGAGGGACCATGAGCCTGTGCCCTCCCCTGCAGCTTTCTCTATCT ACAACTTAAAGAAAGCAAACATCTTTTCCCAGGCCTTTCCCTGACCCCATCTTTGCAGAGAAAGGGTTTCCAGAGGGCAAAGCTGGGACACAGCACAGGT GAATCCTGAAGGCCCTGCTTCTGCTCTGGGGGAGGCTCCAGGACCCTGAGCTGTGAGCACCTGGTTCTCTGGACAGTCCCCAGAGGCCATTTCCACAGCC TTCAGCCACCAGCCACCCCGAGGAGCTGGCTGGACAAGGCTCCAGGGCTTCCAGAGGCCTGGCTTGGACACCTCCCCCAGCTGGCCGTGGAGGGTCACAA CCTGGCCTCTGGGTGGGCAGCCAGCCCTGGAGGGCATCCTCTGCAAGCTGCCTGCCACCCTCATCGGCACTCCCCCACAGGCCTCCCTCTCATGGGTTCC ATGCCCCTTTTTCCCAAGCCGGATCAGGTGAGCTGTCACTGCTGGGGGATCCACCTGCCCAGCCCAGAAGAGGCCACTGAAACGGAAAGGAAAGCTGAGA TTATCCAGCAGCTCTGTTCCCCACCTCAGCGCTTCCTGCCCATGTGGGGAAACAGGTCTGAGAAGGAAGGGGCTTGCCCAGGGTCACACAGGAAGCCTTC AGGCTCTGCTTCTGCCTGATGGCTCTGCTCAGCACATTCACGGTGGAGAGGAGAATTTGGGGGTCACTTGAGGGGGGAAATGTAGGGAATTGTGGGTGGG GAGCAAGGGAAGATCCGTGCACTCGTCCACACCCACCACCACACTCGCTGACACCCACCCCCACACGCTGACACCCACCCCCACACTTGCCCACACCCAT CACCGCACTCGCCCACACCCACCACCACACTGCCCCACACCCACCACCACACTCCCCCACACCCACCACCACACTCGCCCACACCCACCACCAGTGACTT GAGCATCTGTGCTTCGCTGTGACGCCCCTCGCCCTAGGCAGGAACGACGCTGGGAGGAGTCTCCAGGTCAGACCCAGCTTGGAAGCAAGTCTGTCCTCAC TGCCTATCCTTCTGCCATCATAACACCCCCTTCCTGCTCTGCTCCCCGGAATCCTCAGAAACGGGATTTGTATTTGCCGTGACTGGTTGGCCTGAACACG TAGGGCTCCGTGACTGGGACAGGAATGGGCAGGAGAAGCAAGAGTCGGAGCTCCAAGGGGCCCAGGGGTGGCCTGGGGAAGGAAGATGGTCAGCAGGCTG GGGGAGAGGCTCTAGGTGATGAAATATTACATTCCCGACCCCAAGAGAGCACCCACCCTCAGACCTGCCCTCCACCTGGCAGCTGGGGAGCCCTGGCCTG AACCCCCCCCTCCCAGCAGGCCCACCCTCTCTCTGACTTCCCTGCTCTCACCTCCCCGAGAACAGCTAGAGCCCCCTCCTCCGCCTGGCCAGGCCACCAG CTTCTCTTCTGCAAACGTTTGTGCCTCTGAAATGCTCCGTTGTTATTGTTTCAAGACCCTAACTTTTTTTTAAAACTTTCTTAATAAAGGGAAAAGAAAC TTGTAAA

refseq_RSPO4.F1 (forward primer)
refseq_RSPO4.R1 (reverse primer)
Single exon skipping

Frame 0

HSSPRARRAAAGTWGPWAVGPPGERQPGSGCPD AGATLPAPARRPRRGHARPEPKEEASGHWPGGQL HRLYHLLRGERLFHLPAEALPVHPPGRHPPVRQ VPARLSPWVLRHPRPGGQQVQKMWGHL*ELLQP GLLHPVQEAVLLVQGEVSAHLPAGHFGPPEHTGV PGGV*TGSLGRLEPLHTQWKDLRLGLGPGEPGT RGWPGWA*GGSHLPGAF*VKEMSHPEALPRREE PRPEEGQEGPAPTQGQEAGPQAGREAAPARPAAL TAGSPDSLVLVLGPCTPPPAPSPPLLLLFLLCL LHLSSLSFHPSIIFLSVYLPFLFLFYFLYFFHL HSPLLSPSLLPFLPLLSHLSFIFPFLSSCVSSCP SPHPSLSPSSCLPLTHTLRGTMSLCPPLQLSLS TT*RKQTSFPRPFPDPIFAEKGFPEGKAGTQHR *ILKALLLLWGRLQDPEL*APGSLDSPQRPFPQP SATSHPEELAGQGSRASRGLAWTPPPAGRGGSQ PGLWVGSQPWRASSASCLPPSSALPHRPPSHGF HAPFSQAGSGELSLLGDPPAQPRRGH*NGKES*D YPAALFPTSALPAHVGKQV*EGRGLPRVTQEAF RLCFCLMALLSTFTVERRIWGSLEGGNVGNCGW GAREDPCTRPHPPPHSLTPTPTR*HPPPHLPTPI TALAHTHHHTAPHPPPHSPTPTTTLAHTHHQ*L EHLCFAVTPLALGRNDAGRSLQVRPSLEASLSS LPILLPS*HPLPALLPGILRNGICICRDWLA*TR RAP*LGQEWAGEARVGAPRGPGVAWGRKMVSRL GERL*VMKYYIPDPKRAPTLRPALHLAAGEPWP EPPPPSRPTLSLTSLLSPPREQLEPPPPPGQATS FSSANVCASEMLRCYCFKTLTFF*NFLNKGKRN L*

Frame 1

TAAPAPAVPPPGRGALGPSGRLGSASPDPAAQM RAPLCLLLLVAHAVDMLALNRRKKQVGTGLGGN CTGCIICSEENGCSTCQQRLFLFIRREGIRQYGK CLHDCPPGYFGIRGQEVNRCKKCGATCESCFSQ DFCIRCKRQFYLYKGKCLPTCPPGTLAHQNTRE CQGECELGPWGGWSPCTHNGKTCGSAWGLESRVR EAGRAGHEEAATCQVLSESRKCPIQRPCPGERS PGQKKGRKDRRPRKDRKLDRRLDVRPRQPGLQP *PPALPTLWS*SSAPAHLLLLLLLLSSYSFSSVF SICPLFLSTLLSFFCQSTFPFFFFFISFISSTS ILLSFLPPSFPSFLFFLTYLLSFLFFLPVFLPV LHRILLSLPPLVSLSHTL*EGP*ACALPCSFLYL QLKESKHLFPGLSLTPSLQRKGFQRAKLGHSTG ES*RPCFCSGGGSRTLSCEHLVLWTVPRGHFHS LQPPATPRSWLDKAPGLPEAWLGHLPQLAVEGHN LASGWAASPGGHPLQAACHPHRHSPTGLPLMGS MPLFPKPDQVSCHCWGIHLPSPEEATETERKAE IIQQLCSPPQRFLPMWGNRSEKEGACPGSHRKPS GSASA*WLCSAHSRWRGEFGGHLRGEM*GIVGG EQGKIRALVHTHHHTR*HPPPHADTHPHTCPHP SPHSPTPTTTLPHTHHHTPPHPPPHSPTPTTSDL SICASL*RPSP*AGTTLGGVSRSDPAWKQVCPH CLSFCHHNTPFLLCSPESSETGFVFAVTGWPEH VGLRDWDRNGQEKQESELQGAQGWPGEGRWSAGW GRGSR**NITFPTPREHPPSDLPSTWQLGSPGL NPPLPAGPPSL*LPCSHLPENS*SPLLRLARPP ASLLQTFVPLKCSVVIVSRP*LFFKTFLIKGKET CK

Frame 2

QQPPRPPCRRRDVGPLGRRAAWGAPARIRLPRC GRHSACSCSSPTPWTCSP*TEGRSKWALAWGAT AQAVSSAQRRTAVPPASRGSSCSSAGKASASTA SACTTVPLGTSASAARRSTGAKNVGPLVRAASAR TSASGARGSFTCTRGSVCPPARRALWPTRTHGS ARGSVNWVPGAAGAPAHTMERPAARLGAWRAGY ERLAGLGMRRQPPARCFLSQGNVPSRGPAQERGA PARRRAGRTGAHARTGSWTAGWT*GRASPACSP DRRLSRLSGPSPRPLHTSSCSFSSSPLTLSPLS SPFVLSFFPPFYHFSVSLPSLSFSFLFPLFLPPP FSSPFSLPPSLPSSSFSLIFYLSFSFFLCFFLS FTASFSLSLLLSPSHTHFKRDHEPVPSPAAFSI YNLKKANIFSQAFP*PHLCRERVSRGQSWDTAQV NPEGPASALGEAPGP*AVSTWFSGQSPEAISTA FSHQPPRGAGWTRLQGFQRPGLDTSPSWPWRVT TWPLGGQPALEGILCKLPATLIGTPPQASLSWVP CPFFPSRIR*AVTAGGSTCPAQKRPLKRKGKLR LSSSSVPHLSASCPCGETGLRRKGLAQGHTGSL QALLLPDGSAQHIHGGEENLGVT*GGKCRELWVG SKGRSVHSSTPTTTLADTHPHTLTPTPTLAHTH HRTRPHPPPHCPTPTTTLPHTHHHTRPHPPPVT *ASVLRCDAPRPRQERRWEESPGQTQLGSKSVLT AYPSAIITPPSCSAPRNPQKRDLYLP*LVGLNT *GSVTGTGMGRRSKSRSSKGPRGGLGKEDGQQA GGEALGDEILHSRPQESTHPQTCPPPGSWGALA* TPPSQQAHPLSDFPALTSPRTARAPSSAWPGHQ LLFCKRLCL*NAPLLLFQDPNFFLKLS**REKK LV