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Sequence NM_000939
CCTTCCCCTGGCCCGGGGAGCTGCTCCTTGTGCTGCCGGGAAGGTCAAAGTCCCGCGCCCACCAGGAGAGCTCGGCAAGTATATAAGGACAGAGGAGCGCGGGACCAAGCGGCGGCGAAGGAGGGGAAGAAGAGCCGCGACCGAGAGAGGCCGCCGAGCGTCCCCGCCCTCAGAGAGCAGCCTCCCGAGACAGAGCCTCA
GCCTGCCTGGAAGATGCCGAGATCGTGCTGCAGCCGCTCGGGGGCCCTGTTGCTGGCCTTGCTGCTTCAGGCCTCCATGGAAGTGCGTGGCTGGTGCCTG
GAGAGCAGCCAGTGTCAGGACCTCACCACGGAAAGCAACCTGCTGGAGTGCATCCGGGCCTGCAAGCCCGACCTCTCGGCCGAGACTCCCATGTTCCCGG
GAAATGGCGACGAGCAGCCTCTGACCGAGAACCCCCGGAAGTACGTCATGGGCCACTTCCGCTGGGACCGATTCGGCCGCCGCAACAGCAGCAGCAGCGG
CAGCAGCGGCGCAGGGCAGAAGCGCGAGGACGTCTCAGCGGGCGAAGACTGCGGCCCGCTGCCTGAGGGCGGCCCCGAGCCCCGCAGCGATGGTGCCAAG
CCGGGCCCGCGCGAGGGCAAGCGCTCCTACTCCATGGAGCACTTCCGCTGGGGCAAGCCGGTGGGCAAGAAGCGGCGCCCAGTGAAGGTGTACCCTAACG
GCGCCGAGGACGAGTCGGCCGAGGCCTTCCCCCTGGAGTTCAAGAGGGAGCTGACTGGCCAGCGACTCCGGGAGGGAGATGGCCCCGACGGCCCTGCCGA
TGACGGCGCAGGGGCCCAGGCCGACCTGGAGCACAGCCTGCTGGTGGCGGCCGAGAAGAAGGACGAGGGCCCCTACAGGATGGAGCACTTCCGCTGGGGC
AGCCCGCCCAAGGACAAGCGCTACGGCGGTTTCATGACCTCCGAGAAGAGCCAGACGCCCCTGGTGACGCTGTTCAAAAACGCCATCATCAAGAACGCCT
ACAAGAAGGGCGAGTGAGGGCACAGCGGGGCCCCAGGGCTACCCTCCCCCAGGAGGTCGACCCCAAAGCCCCTTGCTCTCCCCTGCCCTGCTGCCGCCTC
CCAGCCTGGGGGGTCGTGGCAGATAATCAGCCTCTTAAAGCTGCCTGTAGTTAGGAAATAAAACCTTTCAAATTTCACATCCACCTCTGACTTTGAATGT
AAACTGTGTGAATAAAGTAAAAATACGTAGCCGTCAAATAACAGC
- refseq_POMC.F1 (forward primer)
- refseq_POMC.R1 (reverse primer)
- Single exon skipping
Frame 0
PSPGPGSCSLCCREGQSPAPTRRARQVYKDRGARDQAAAKEGKKSRDRERPPSVPALREQPPETEPQ
PAWKMPRSCCSRSGALLLALLLQASMEVRGWCL
ESSQCQDLTTESNLLECIRACKPDLSAETPMFP
GNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSG
SSGAGQKREDVSAGEDCGPLPEGGPEPRSDGAK
PGPREGKRSYSMEHFRWGKPVGKKRRPVKVYPN
GAEDESAEAFPLEFKRELTGQRLREGDGPDGPAD
DGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWG
SPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNA
YKKGE*GHSGAPGLPSPRRSTPKPLALPCPAAAS
QPGGSWQIISLLKLPVVRK*NLSNFTSTSDFEC
KLCE*SKNT*PSNNS
Frame 1
LPLARGAAPCAAGKVKVPRPPGELGKYIRTEERGTKRRRRRGRRAATERGRRASPPSESSLPRQSL
SLPGRCRDRAAAARGPCCWPCCFRPPWKCVAGAW
RAASVRTSPRKATCWSASGPASPTSRPRLPCSR
EMATSSL*PRTPGSTSWATSAGTDSAAATAAAA
AAAAQGRSARTSQRAKTAARCLRAAPSPAAMVPS
RARARASAPTPWSTSAGASRWARSGAQ*RCTLT
APRTSRPRPSPWSSRGS*LASDSGREMAPTALP
MTAQGPRPTWSTACWWRPRRRTRAPTGWSTSAGA
ARPRTSATAVS*PPRRARRPW*RCSKTPSSRTP
TRRASEGTAGPQGYPPPGGRPQSPLLSPALLPP
PSLGGRGR*SAS*SCL*LGNKTFQISHPPLTLNV
NCVNKVKIRSRQIT
Frame 2
FPWPGELLLVLPGRSKSRAHQESSASI*GQRSAGPSGGEGGEEEPRPREAAERPRPQRAASRDRAS
ACLEDAEIVLQPLGGPVAGLAASGLHGSAWLVP
GEQPVSGPHHGKQPAGVHPGLQARPLGRDSHVPG
KWRRAASDREPPEVRHGPLPLGPIRPPQQQQQR
QQRRRAEARGRLSGRRLRPAA*GRPRAPQRWCQ
AGPARGQALLLHGALPLGQAGGQEAAPSEGVP*R
RRGRVGRGLPPGVQEGADWPATPGGRWPRRPCR
*RRRGPGRPGAQPAGGGREEGRGPLQDGALPLG
QPAQGQALRRFHDLREEPDAPGDAVQKRHHQERL
QEGRVRAQRGPRATLPQEVDPKAPCSPLPCCRL
PAWGVVADNQPLKAACS*EIKPFKFHIHL*L*M
*TV*IK*KYVAVK*Q
Sequence NM_001035256
CCTTCCCCTGGCCCGGGGAGCTGCTCCTTGTGCTGCCGGGAAGGTCAAAGTCCCGCGCCCACCAGGAGAGCTCGGCAAGTATATAAGGACAGAGGAGCGCGGGACCAAGCGGCGGCGAAGGAGGGGAAGAAGAGCCGCGACCGAGAGAGGCCGCCGAGCGTCCCCGCCCTCAGAGAGCAGCCTCCCGAGACAGGCACTTG
CTGGATTCTCCAAAAGTATCTGCAGTGGCTGTTCCACCAGGAGAGCCTCAGCCTGCCTGGAAGATGCCGAGATCGTGCTGCAGCCGCTCGGGGGCCCTGT
TGCTGGCCTTGCTGCTTCAGGCCTCCATGGAAGTGCGTGGCTGGTGCCTGGAGAGCAGCCAGTGTCAGGACCTCACCACGGAAAGCAACCTGCTGGAGTG
CATCCGGGCCTGCAAGCCCGACCTCTCGGCCGAGACTCCCATGTTCCCGGGAAATGGCGACGAGCAGCCTCTGACCGAGAACCCCCGGAAGTACGTCATG
GGCCACTTCCGCTGGGACCGATTCGGCCGCCGCAACAGCAGCAGCAGCGGCAGCAGCGGCGCAGGGCAGAAGCGCGAGGACGTCTCAGCGGGCGAAGACT
GCGGCCCGCTGCCTGAGGGCGGCCCCGAGCCCCGCAGCGATGGTGCCAAGCCGGGCCCGCGCGAGGGCAAGCGCTCCTACTCCATGGAGCACTTCCGCTG
GGGCAAGCCGGTGGGCAAGAAGCGGCGCCCAGTGAAGGTGTACCCTAACGGCGCCGAGGACGAGTCGGCCGAGGCCTTCCCCCTGGAGTTCAAGAGGGAG
CTGACTGGCCAGCGACTCCGGGAGGGAGATGGCCCCGACGGCCCTGCCGATGACGGCGCAGGGGCCCAGGCCGACCTGGAGCACAGCCTGCTGGTGGCGG
CCGAGAAGAAGGACGAGGGCCCCTACAGGATGGAGCACTTCCGCTGGGGCAGCCCGCCCAAGGACAAGCGCTACGGCGGTTTCATGACCTCCGAGAAGAG
CCAGACGCCCCTGGTGACGCTGTTCAAAAACGCCATCATCAAGAACGCCTACAAGAAGGGCGAGTGAGGGCACAGCGGGGCCCCAGGGCTACCCTCCCCC
AGGAGGTCGACCCCAAAGCCCCTTGCTCTCCCCTGCCCTGCTGCCGCCTCCCAGCCTGGGGGGTCGTGGCAGATAATCAGCCTCTTAAAGCTGCCTGTAG
TTAGGAAATAAAACCTTTCAAATTTCACATCCACCTCTGACTTTGAATGTAAACTGTGTGAATAAAGTAAAAATACGTAGCCGTCAAATAACAGC
- refseq_POMC.F1 (forward primer)
- refseq_POMC.R1 (reverse primer)
- Single exon skipping
Frame 0
PSPGPGSCSLCCREGQSPAPTRRARQVYKDRGARDQAAAKEGKKSRDRERPPSVPALREQPPETGTC
WILQKYLQWLFHQESLSLPGRCRDRAAAARGPC
CWPCCFRPPWKCVAGAWRAASVRTSPRKATCWS
ASGPASPTSRPRLPCSREMATSSL*PRTPGSTSW
ATSAGTDSAAATAAAAAAAAQGRSARTSQRAKT
AARCLRAAPSPAAMVPSRARARASAPTPWSTSA
GASRWARSGAQ*RCTLTAPRTSRPRPSPWSSRGS
*LASDSGREMAPTALPMTAQGPRPTWSTACWWR
PRRRTRAPTGWSTSAGAARPRTSATAVS*PPRR
ARRPW*RCSKTPSSRTPTRRASEGTAGPQGYPPP
GGRPQSPLLSPALLPPPSLGGRGR*SAS*SCL*
LGNKTFQISHPPLTLNVNCVNKVKIRSRQIT
Frame 1
LPLARGAAPCAAGKVKVPRPPGELGKYIRTEERGTKRRRRRGRRAATERGRRASPPSESSLPRQAL
AGFSKSICSGCSTRRASACLEDAEIVLQPLGGPV
AGLAASGLHGSAWLVPGEQPVSGPHHGKQPAGV
HPGLQARPLGRDSHVPGKWRRAASDREPPEVRH
GPLPLGPIRPPQQQQQRQQRRRAEARGRLSGRRL
RPAA*GRPRAPQRWCQAGPARGQALLLHGALPL
GQAGGQEAAPSEGVP*RRRGRVGRGLPPGVQEG
ADWPATPGGRWPRRPCR*RRRGPGRPGAQPAGGG
REEGRGPLQDGALPLGQPAQGQALRRFHDLREE
PDAPGDAVQKRHHQERLQEGRVRAQRGPRATLP
QEVDPKAPCSPLPCCRLPAWGVVADNQPLKAACS
*EIKPFKFHIHL*L*M*TV*IK*KYVAVK*Q
Frame 2
FPWPGELLLVLPGRSKSRAHQESSASI*GQRSAGPSGGEGGEEEPRPREAAERPRPQRAASRDRHL
LDSPKVSAVAVPPGEPQPAWKMPRSCCSRSGAL
LLALLLQASMEVRGWCLESSQCQDLTTESNLLEC
IRACKPDLSAETPMFPGNGDEQPLTENPRKYVM
GHFRWDRFGRRNSSSSGSSGAGQKREDVSAGED
CGPLPEGGPEPRSDGAKPGPREGKRSYSMEHFRW
GKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRE
LTGQRLREGDGPDGPADDGAGAQADLEHSLLVA
AEKKDEGPYRMEHFRWGSPPKDKRYGGFMTSEKS
QTPLVTLFKNAIIKNAYKKGE*GHSGAPGLPSP
RRSTPKPLALPCPAAASQPGGSWQIISLLKLPV
VRK*NLSNFTSTSDFECKLCE*SKNT*PSNNS
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