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Sequences

Sequence NM_138338

GCGCAAGCGCGAGCGGCTGTGCGCGGTGCGTGGTGCGTGGTGCGAGAGCCCCCAGCGCAGGCCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCC
GGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTG
CATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCAT
CCATTCC
TAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAG
AGTCACTGCAGCA
GCCAGCCAAGTTCGACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGG
CGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCA
AAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAGCCCTGGGGCTGGACAGTGG
ACCCTACCAGCCTGCGGGAAGGTGGTATGGCCGGCTGTGAAGACAACAGCAGCTGAGGCCGATGCTAAGGAGATAGTGTCTCGAGCTGGACAGTGAGGCA
AGGCCAGGAGTGCCCACCAGCTTGCCCCGCTCCTCCACCATCTAGGCCCTGCTCCTCCTTCACTGCAGTGATGGATCCATCTGTGGCTGTGGCTGTTGGG
CCTCCACTGACCTTGACTGAAAAGACCCTTCCTGGACCCTGGACTTGACAACAAACGGCACAGACACCAATCACTCACAGAACAGTCACTGGTTTTGGAT
TCTTGTTTTCCTTGCAAGAACAAAAGGTTCTCTAGAATCCTTCAGCCACCAATGAAATGATCAGGCAGGAGAAAAACTTTAGATGGGAGTCTTGTCATCA
AAAATAAATACAGTTGCATGGAGCTGCTTAAGATCTGAACCAAACTCAAAATAGGAAGACGGGGGCTAAAAAGTTATTTTAAGAACCCCCCCACCACAAC
CACAGTCACTCCGTGGGCTAAGCGGGGAGCAGGAAGCATGTCTGGTCACACCATCTTGGAATAGGAAGCCATGGCTGGACGGATCGGATCCACTGATGGC
ACACGTGGAGCTGAACTTGACGCCAGCGGCGGGGCGGGGAGGCACTGCCCTCACTGTTGCAGTTCCTTCATTCTGTTGAGGGCACTGCCAGCGCGGAACC
ACTCAATCTGCGTCTCGTTGAAGGTGTGGTTCAGGAGGATGGTCTCCTGGGTCCCGTTGGGGTGCTTGATGATGCACTTCAGGGGCTGCAAGGAGAAGGT
GGGTGTGGGTGGAAGTGGTGGGTACTGTGTGCCCTTGGCCCCGCAGGGGGGTCAGGGAGACAGGGCACTGCCTGGGGACCTAAGAGGTCAGCAGGCAAAC
CAAATCCTAGGCCAGTCTGTGCCTGATGGCCCAGGTCAGACCCAGGTGGCACCTACAGGGGATTCCTGGGGCCCAGAGGGTCCTGCAGTGGGTGGGCTGT
CCCCTGAGTGCCAGGTCCTGGTGAGTAAAGGGAGGTTGGGGAGGTGTGAGGGCCCCGGGTTGACTCCAACCCCCAACTCTGAGAAGCCACTTTGCTTTCT
CTGTGGGGCCACCTGGAGAACTGGGGGCCTTTCCAGCTGGAATGAAGCCTCCTGGGAGAAGCTACCCTGGGGCCAGTGCAGGAGGGGAAACAAGGTGGCT
GCCCCTCACCCCACCACCTCAGGGGGACCCGGGCCCCTAACCTTGCCAGGGGTGAAGTCCTTCAGGCCCTGAATGGTCAGCTTGTCCACAGGGTGAATCT
TGTTGTAGTCAGCCGGGTCAGCGAAGGTCAGAGGCAGCAGGCCCTGTTTCTTCAGGTTGGTCTCTGGAAAGCAGGCGGCATTCGGTCATCGGGGGCCTGA
CTAGCCTGGAGAGCTTCATTTTCTGAACCCATCTGGGGCTCTGGGAGATGCCCCTAATCCTGCTGCCTGGGACAATGCCCGGGCAGTGACTATGGGGCCC
TGGTCTGCTCCCTTTCACTAGGGGTGCCCCTAGCAAGTGTGCGCTGGTGCTGAGGTCTGCGAGGCCTTCCTGCTGCACATTCATGATCAGAAAGAACCCC
CTGGCACATGTCCTAGATCTCAGCGGCTCAGATCTACAAGCCTGGAAGCAGGGCCTGCGGGAAGCCGGGAAGCTGAGCGTCGGATGTGCACTGTGTGAGC
CTAAGAAGGGGCAAGAGGCTGACGGGCCTGTGGGCTGCAGTGGACACAGCCTAGAACCAGGCCCAACTCAGCCACGGGCAGGGAGAGTGACCTTGATCAC
TAGGGATGGGATGAGGATGGCCTGGGTACAGACTCCAGCTCACCGTGGATCCTGGCAAAGCTCTTGGTGATGATGGCCCGGCCCCCAAGGTGGCGAGGCT
CCAGAGCTGCATGCTCCCGGCTCGAGCCCTCGCCGTAGTTCTCGTCTCCGATCACCACCCACCTGATGCCATGTTTCTGTCAGGCAAGCGGGGGTAAGGT
TATAAGGCAGAGGAGGGCACCGTCCTCACCTGTCTGGCCCTACCTGCCTGGCCTCACCCACCTGGCGCCTGGGCATCCTGAGGCTCCCGGTAAAGGGGCT
GGAGGGGCCCTTCCTCGCCTGCTGCCTAAGGGCAAGTGAAGCCTGCGTGAATGAGACCCAAACGAGGCCCCGAGGGGACAGAGGCACGTGGTTGTTTGCA
GTGGCAAAGAAGACAGAAGCCACCCATTGGTGCTTGGCAGAGGCAGGTGTGTGTGGAGGGAGGTTGGGTGAGACAGGGACGGCCTCCGGGCCCCACACAA
GATGCATCTGTGAAGCTCAAGGCGCCCCCTTCCTGGGAGCACCCTCTGCCCCACCCAGCCACCAGGGGGTGCCAGAGTCCTTTCACCCGGCTGGGCCAGG
ACTTGGGTTCAGCCCTGGTTCTGTTTGTTTCTCAAAGCAGAAAAAGTCCAATCACCTCAGACCCTCTCTGCCTGCTCACTGTGAGGCCCCGTGTAGCCCC
CTCTCTGAGCTTTTGGCCTCTGACTTCCTGAGTCGGCCCCCTTCCTTGTCTTTGTCTAGGGCCAGATATCCTAAGATATCTCTCACTAACTCCTCCTGGT
GCAGGGACCCGGCTGTACCACCGGACCTGGGCCTCATGCCTTCTCAGCCACAGGGAGGGGCTGCAGGCCTCTCCTCCCTTCCACAGCAGTCCCCTTTGGG
CTTGGTCGGCCTCCTTCCCAGTTTCCACTCCCCTAATGTCCTCCCTGCACCTGCCTTGCAGGCCCCTTCAGGAGTGACCACTGGCATTGCCCCTATCAAC
TCTGACCCACCTTGTAGTAGCGGGCAGTGTCAGGGACGGGGCCAAACTCCTGAGTGACGGCATTGCGCACGGAGTTGGCCTTGCCGTTTTCAATGTTGAT
GGCACCAATGAGCAGGTTGTTGGAGATGTTATCCAAGTGCCCACGGAACTTGAGCCAGGGGCCAGCAGCTGAGATGTGGTCAGTGGTACACTTCCCTTTG
ACCTTGGGTGGTAAGTAGAGAGGACAGAGGTCAGGGCATGGCCCTGGAGGGGTGGATGACAAGCCCTCCAGAGGCAGGCAGCAGGCCCCGGGGCCCAGGA
GGGGTGACAGAGGGGTGAGAGGCAGGCAAGTCTTCAGACACGTGTTCTGTGGTCCTGGTGCTTCCACCCTAAGGCCACATGGGCTCAGGCCAGGCCTTCA
TCCCCTCAAAGGCCTCTGGCTGCTCAGTCTCCCTTATGGGGCAGAGGCCGCCCAAAGTTGGGACAGGTCAATGTTCGCTCACCCTTCAGGCCTCAGCTGC
AAGATCAGCCACAGACCTCATGCTGGACACAGGAGCCCAGGACATGCCTGGGTCCTGGCTGATGACTGATTAGTCTCTTCTGTCACAGGGGTGCCTGGAG
ACCCCTTAATAAGCAGCATAAATAAATATCTGACACAGATTGGAGGCCTCTGGCCACACCAAAAA
  • refseq_POLR3H.F1 (forward primer)
  • refseq_POLR3H.R1 (reverse primer)
  • Alternative donor

Frame 0

AQARAAVRGAWCVVREPPAQAGMFVLVEMVDTV
RIPPWQFERKLNDSIAEELNKKLANKVVYNVGLC
ICLFDITKLEDAYVFPGDGASHTKVHFRCVVFH
PF
LDEILIGKIKGCSPEGVHVSLGFFDDILIPP
ESLQQ
PAKFDEAEQVWVWEYETEEGAHDLYMDTG
EEIRFRVVDESFVDTSPTGPSSADATTSSEELP
KKEAPYTLVGSISEPGLGLLSWWTSN*PWGWTV
DPTSLREGGMAGCEDNSS*GRC*GDSVSSWTVRQ
GQECPPACPAPPPSRPCSSFTAVMDPSVAVAVG
PPLTLTEKTLPGPWT*QQTAQTPITHRTVTGFG
FLFSLQEQKVL*NPSATNEMIRQEKNFRWESCHQ
K*IQLHGAA*DLNQTQNRKTGAKKLF*EPPHHN
HSHSVG*AGSRKHVWSHHLGIGSHGWTDRIH*W
HTWS*T*RQRRGGEALPSLLQFLHSVEGTASAEP
LNLRLVEGVVQEDGLLGPVGVLDDALQGLQGEG
GCGWKWWVLCALGPAGGSGRQGTAWGPKRSAGK
PNPRPVCA*WPRSDPGGTYRGFLGPRGSCSGWAV
P*VPGPGE*REVGEV*GPRVDSNPQL*EATLLS
LWGHLENWGPFQLE*SLLGEATLGPVQEGKQGG
CPSPHHLRGTRAPNLARGEVLQALNGQLVHRVNL
VVVSRVSEGQRQQALFLQVGLWKAGGIRSSGA*
LAWRASFSEPIWGSGRCP*SCCLGQCPGSDYGA
LVCSLSLGVPLASVRWC*GLRGLPAAHS*SERTP
WHMS*ISAAQIYKPGSRACGKPGS*ASDVHCVS
LRRGKRLTGLWAAVDTA*NQAQLSHGQGE*P*S
LGMG*GWPGYRLQLTVDPGKALGDDGPAPKVARL
QSCMLPARALAVVLVSDHHPPDAMFLSGKRG*G
YKAEEGTVLTCLALPAWPHPPGAWAS*GSR*RG
WRGPSSPAA*GQVKPA*MRPKRGPEGTEARGCLQ
WQRRQKPPIGAWQRQVCVEGGWVRQGRPPGPTQ
DASVKLKAPPSWEHPLPHPATRGCQSPFTRLGQ
DLGSALVLFVSQSRKSPITSDPLCLLTVRPRVAP
SLSFWPLTS*VGPLPCLCLGPDILRYLSLTPPG
AGTRLYHRTWASCLLSHREGLQASPPFHSSPLW
AWSASFPVSTPLMSSLHLPCRPLQE*PLALPLST
LTHLVVAGSVRDGAKLLSDGIAHGVGLAVFNVD
GTNEQVVGDVIQVPTELEPGASS*DVVSGTLPF
DLGW*VERTEVRAWPWRGG*QALQRQAAGPGAQE
G*QRGERQASLQTRVLWSWCFHPKATWAQARPS
SPQRPLAAQSPLWGRGRPKLGQVNVRSPFRPQL
QDQPQTSCWTQEPRTCLGPG**LISLFCHRGAWR
PLNKQHK*ISDTDWRPLATPK

Frame 1

RKRERLCAVRGAWCESPQRRPGCSSWWKWWTPS
GSPLGSLRGSSTTPLPRS*TRSWPTRSCTTWDS
AFVCLISPNWRMPMYSLGMAHHTPKSIFAAWCFI
HS
*MRFSLGRSKAAAQKECTSL*ASSMTFSSPQ
SHCS
SQPSSTKRSRCGCGSTRRRKEHTTSTWTP
ARRSASGWWTRALLTRPPQGPAQQMPPLPVRSCQ
RRRLRTRLWDPSVSQAWAFSPGGPATSPGAGQW
TLPACGKVVWPAVKTTAAEADAKEIVSRAGQ*G
KARSAHQLAPLLHHLGPAPPSLQ*WIHLWLWLLG
LH*P*LKRPFLDPGLDNKRHRHQSLTEQSLVLD
SCFPCKNKRFSRILQPPMK*SGRRKTLDGSLVI
KNKYSCMELLKI*TKLKIGRRGLKSYFKNPPTTT
TVTPWAKRGAGSMSGHTILE*EAMAGRIGSTDG
TRGAELDASGGAGRHCPHCCSSFILLRALPARN
HSICVSLKVWFRRMVSWVPLGCLMMHFRGCKEKV
GVGGSGGYCVPLAPQGGQGDRALPGDLRGQQAN
QILGQSVPDGPGQTQVAPTGDSWGPEGPAVGGL
SPECQVLVSKGRLGRCEGPGLTPTPNSEKPLCFL
CGATWRTGGLSSWNEASWEKLPWGQCRRGNKVA
APHPTTSGGPGPLTLPGVKSFRP*MVSLSTG*I
LL*SAGSAKVRGSRPCFFRLVSGKQAAFGHRGPD
*PGELHFLNPSGALGDAPNPAAWDNARAVTMGP
WSAPFH*GCP*QVCAGAEVCEAFLLHIHDQKEP
PGTCPRSQRLRSTSLEAGPAGSREAERRMCTV*A
*EGARG*RACGLQWTQPRTRPNSATGRESDLDH
*GWDEDGLGTDSSSPWILAKLLVMMARPPRWRG
SRAACSRLEPSP*FSSPITTHLMPCFCQASGGKV
IRQRRAPSSPVWPYLPGLTHLAPGHPEAPGKGA
GGALPRLLPKGK*SLRE*DPNEAPRGQRHVVVC
SGKEDRSHPLVLGRGRCVWREVG*DRDGLRAPHK
MHL*SSRRPLPGSTLCPTQPPGGARVLSPGWAR
TWVQPWFCLFLKAEKVQSPQTLSACSL*GPV*P
PL*AFGL*LPESAPFLVFV*GQIS*DISH*LLLV
QGPGCTTGPGPHAFSATGRGCRPLLPSTAVPFG
LGRPPSQFPLP*CPPCTCLAGPFRSDHWHCPYQ
L*PTL**RAVSGTGPNS*VTALRTELALPFSMLM
APMSRLLEMLSKCPRNLSQGPAAEMWSVVHFPL
TLGGK*RGQRSGHGPGGVDDKPSRGRQQAPGPR
RGDRGVRGRQVFRHVFCGPGASTLRPHGLRPGLH
PLKGLWLLSLPYGAEAAQSWDRSMFAHPSGLSC
KISHRPHAGHRSPGHAWVLADD*LVSSVTGVPG
DPLISSINKYLTQIGGLWPHQK

Frame 2

ASASGCARCVVRGARAPSAGRDVRPGGNGGHRP
DPPLAV*EEAQRLHCRGAEQEVGQQGRVQRGTL
HLSV*YHQTGGCLCIPWGWRITHQSPFSLRGVS
SIP
R*DSHWEDQRLQPRRSARLSRLLR*HSHPPR
VTAA
ASQVRRSGAGVGVGVRDGGRSTRPLHGHR
RGDPLPGGGRELC*HVPHRAQLSRCHHFQ*GAA
KEGGSVHACGIHQ*ARPGPSLLVDQQLALGLDSG
PYQPAGRWYGRL*RQQQLRPMLRR*CLELDSEA
RPGVPTSLPRSSTI*ALLLLHCSDGSICGCGCW
ASTDLD*KDPSWTLDLTTNGTDTNHSQNSHWFWI
LVFLARTKGSLESFSHQ*NDQAGEKL*MGVLSS
KINTVAWSCLRSEPNSK*EDGG*KVILRTPPPQ
PQSLRGLSGEQEACLVTPSWNRKPWLDGSDPLMA
HVELNLTPAAGRGGTALTVAVPSFC*GHCQRGT
TQSASR*RCGSGGWSPGSRWGA**CTSGAARRR
WVWVEVVGTVCPWPRRGVRETGHCLGT*EVSRQT
KS*ASLCLMAQVRPRWHLQGIPGAQRVLQWVGC
PLSARSW*VKGGWGGVRAPG*LQPPTLRSHFAF
SVGPPGELGAFPAGMKPPGRSYPGASAGGETRWL
PLTPPPQGDPGP*PCQG*SPSGPEWSACPQGES
CCSQPGQRRSEAAGPVSSGWSLESRRHSVIGGL
TSLESFIF*THLGLWEMPLILLPGTMPGQ*LWGP
GLLPFTRGAPSKCALVLRSARPSCCTFMIRKNP
LAHVLDLSGSDLQAWKQGLREAGKLSVGCALCE
PKKGQEADGPVGCSGHSLEPGPTQPRAGRVTLIT
RDGMRMAWVQTPAHRGSWQSSW**WPGPQGGEA
PELHAPGSSPRRSSRLRSPPT*CHVSVRQAGVR
L*GRGGHRPHLSGPTCLASPTWRLGILRLPVKGL
EGPFLACCLRASEACVNETQTRPRGDRGTWLFA
VAKKTEATHWCLAEAGVCGGRLGETGTASGPHT
RCICEAQGAPFLGAPSAPPSHQGVPESFHPAGPG
LGFSPGSVCFSKQKKSNHLRPSLPAHCEAPCSP
LSELLASDFLSRPPSLSLSRARYPKISLTNSSW
CRDPAVPPDLGLMPSQPQGGAAGLSSLPQQSPLG
LVGLLPSFHSPNVLPAPALQAPSGVTTGIAPIN
SDPPCSSGQCQGRGQTPE*RHCARSWPCRFQC*
WHQ*AGCWRCYPSAHGT*ARGQQLRCGQWYTSL*
PWVVSREDRGQGMALEGWMTSPPEAGSRPRGPG
GVTEG*EAGKSSDTCSVVLVLPP*GHMGSGQAF
IPSKASGCSVSLMGQRPPKVGTGQCSLTLQASAA
RSATDLMLDTGAQDMPGSWLMTD*SLLSQGCLE
TP**AA*INI*HRLEASGHTK

Sequence NM_001018050

GCGCAAGCGCGAGCGGCTGTGCGCGGTGCGTGGTGCGTGGTGCGTGATACGAGCGGCGGCACGTGGCGCGGGTGCGGGGCGTGGAGTGGCGTGGCGTGGA
GTGGCGTGGCGTGGCGGGGTCTCGCGGCGCGGGCGCGCACCCGGAGCTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGTTTCCTCATGTTGTAGTAGCG
GAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCAACCTCACCGCCAGCCTGGGGGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTC
TCCCTGCCCCCGGCCCTGGTGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGGCCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCG
GATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTGC
ATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATC
CATTCC
TAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGA
GTCACTGCAGCA
GCCAGCCAAGTTCGACGAAGCGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGGC
GAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCAA
AGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAGGCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAGCCCTGGGGCTGGACAGTGGA
CCCTACCAGCCTGCGGGAAGGTGGTATGGCCGGCTGTGAAGACAACAGCAGCTGAGGCCGATGCTAAGGAGATAGTGTCTCGAGCTGGACAGTGAGGCAA
GGCCAGGAGTGCCCACCAGCTTGCCCCGCTCCTCCACCATCTAGGCCCTGCTCCTCCTTCACTGCAGTGATGGATCCATCTGTGGCTGTGGCTGTTGGGC
CTCCACTGACCTTGACTGAAAAGACCCTTCCTGGACCCTGGACTTGACAACAAACGGCACAGACACCAATCACTCACAGAACAGTCACTGGTTTTGGATT
CTTGTTTTCCTTGCAAGAACAAAAGGTTCTCTAGAATCCTTCAGCCACCAATGAAATGATCAGGCAGGAGAAAAACTTTAGATGGGAGTCTTGTCATCAA
AAATAAATACAGTTGCATGGAGCTGCTTAAGATCTGAACCAAACTCAAAATAGGAAGACGGGGGCTAAAAAGTTATTTTAAGAACCCCCCCACCACAACC
ACAGTCACTCCGTGGGCTAAGCGGGGAGCAGGAAGCATGTCTGGTCACACCATCTTGGAATAGGAAGCCATGGCTGGACGGATCGGATCCACTGATGGCA
CACGTGGAGCTGAACTTGACGCCAGCGGCGGGGCGGGGAGGCACTGCCCTCACTGTTGCAGTTCCTTCATTCTGTTGAGGGCACTGCCAGCGCGGAACCA
CTCAATCTGCGTCTCGTTGAAGGTGTGGTTCAGGAGGATGGTCTCCTGGGTCCCGTTGGGGTGCTTGATGATGCACTTCAGGGGCTGCAAGGAGAAGGTG
GGTGTGGGTGGAAGTGGTGGGTACTGTGTGCCCTTGGCCCCGCAGGGGGGTCAGGGAGACAGGGCACTGCCTGGGGACCTAAGAGGTCAGCAGGCAAACC
AAATCCTAGGCCAGTCTGTGCCTGATGGCCCAGGTCAGACCCAGGTGGCACCTACAGGGGATTCCTGGGGCCCAGAGGGTCCTGCAGTGGGTGGGCTGTC
CCCTGAGTGCCAGGTCCTGGTGAGTAAAGGGAGGTTGGGGAGGTGTGAGGGCCCCGGGTTGACTCCAACCCCCAACTCTGAGAAGCCACTTTGCTTTCTC
TGTGGGGCCACCTGGAGAACTGGGGGCCTTTCCAGCTGGAATGAAGCCTCCTGGGAGAAGCTACCCTGGGGCCAGTGCAGGAGGGGAAACAAGGTGGCTG
CCCCTCACCCCACCACCTCAGGGGGACCCGGGCCCCTAACCTTGCCAGGGGTGAAGTCCTTCAGGCCCTGAATGGTCAGCTTGTCCACAGGGTGAATCTT
GTTGTAGTCAGCCGGGTCAGCGAAGGTCAGAGGCAGCAGGCCCTGTTTCTTCAGGTTGGTCTCTGGAAAGCAGGCGGCATTCGGTCATCGGGGGCCTGAC
TAGCCTGGAGAGCTTCATTTTCTGAACCCATCTGGGGCTCTGGGAGATGCCCCTAATCCTGCTGCCTGGGACAATGCCCGGGCAGTGACTATGGGGCCCT
GGTCTGCTCCCTTTCACTAGGGGTGCCCCTAGCAAGTGTGCGCTGGTGCTGAGGTCTGCGAGGCCTTCCTGCTGCACATTCATGATCAGAAAGAACCCCC
TGGCACATGTCCTAGATCTCAGCGGCTCAGATCTACAAGCCTGGAAGCAGGGCCTGCGGGAAGCCGGGAAGCTGAGCGTCGGATGTGCACTGTGTGAGCC
TAAGAAGGGGCAAGAGGCTGACGGGCCTGTGGGCTGCAGTGGACACAGCCTAGAACCAGGCCCAACTCAGCCACGGGCAGGGAGAGTGACCTTGATCACT
AGGGATGGGATGAGGATGGCCTGGGTACAGACTCCAGCTCACCGTGGATCCTGGCAAAGCTCTTGGTGATGATGGCCCGGCCCCCAAGGTGGCGAGGCTC
CAGAGCTGCATGCTCCCGGCTCGAGCCCTCGCCGTAGTTCTCGTCTCCGATCACCACCCACCTGATGCCATGTTTCTGTCAGGCAAGCGGGGGTAAGGTT
ATAAGGCAGAGGAGGGCACCGTCCTCACCTGTCTGGCCCTACCTGCCTGGCCTCACCCACCTGGCGCCTGGGCATCCTGAGGCTCCCGGTAAAGGGGCTG
GAGGGGCCCTTCCTCGCCTGCTGCCTAAGGGCAAGTGAAGCCTGCGTGAATGAGACCCAAACGAGGCCCCGAGGGGACAGAGGCACGTGGTTGTTTGCAG
TGGCAAAGAAGACAGAAGCCACCCATTGGTGCTTGGCAGAGGCAGGTGTGTGTGGAGGGAGGTTGGGTGAGACAGGGACGGCCTCCGGGCCCCACACAAG
ATGCATCTGTGAAGCTCAAGGCGCCCCCTTCCTGGGAGCACCCTCTGCCCCACCCAGCCACCAGGGGGTGCCAGAGTCCTTTCACCCGGCTGGGCCAGGA
CTTGGGTTCAGCCCTGGTTCTGTTTGTTTCTCAAAGCAGAAAAAGTCCAATCACCTCAGACCCTCTCTGCCTGCTCACTGTGAGGCCCCGTGTAGCCCCC
TCTCTGAGCTTTTGGCCTCTGACTTCCTGAGTCGGCCCCCTTCCTTGTCTTTGTCTAGGGCCAGATATCCTAAGATATCTCTCACTAACTCCTCCTGGTG
CAGGGACCCGGCTGTACCACCGGACCTGGGCCTCATGCCTTCTCAGCCACAGGGAGGGGCTGCAGGCCTCTCCTCCCTTCCACAGCAGTCCCCTTTGGGC
TTGGTCGGCCTCCTTCCCAGTTTCCACTCCCCTAATGTCCTCCCTGCACCTGCCTTGCAGGCCCCTTCAGGAGTGACCACTGGCATTGCCCCTATCAACT
CTGACCCACCTTGTAGTAGCGGGCAGTGTCAGGGACGGGGCCAAACTCCTGAGTGACGGCATTGCGCACGGAGTTGGCCTTGCCGTTTTCAATGTTGATG
GCACCAATGAGCAGGTTGTTGGAGATGTTATCCAAGTGCCCACGGAACTTGAGCCAGGGGCCAGCAGCTGAGATGTGGTCAGTGGTACACTTCCCTTTGA
CCTTGGGTGGTAAGTAGAGAGGACAGAGGTCAGGGCATGGCCCTGGAGGGGTGGATGACAAGCCCTCCAGAGGCAGGCAGCAGGCCCCGGGGCCCAGGAG
GGGTGACAGAGGGGTGAGAGGCAGGCAAGTCTTCAGACACGTGTTCTGTGGTCCTGGTGCTTCCACCCTAAGGCCACATGGGCTCAGGCCAGGCCTTCAT
CCCCTCAAAGGCCTCTGGCTGCTCAGTCTCCCTTATGGGGCAGAGGCCGCCCAAAGTTGGGACAGGTCAATGTTCGCTCACCCTTCAGGCCTCAGCTGCA
AGATCAGCCACAGACCTCATGCTGGACACAGGAGCCCAGGACATGCCTGGGTCCTGGCTGATGACTGATTAGTCTCTTCTGTCACAGGGGTGCCTGGAGA
CCCCTTAATAAGCAGCATAAATAAATATCTGACACAGATTGGAGGCCTCTGGCCACACCAAAAA
  • refseq_POLR3H.F1 (forward primer)
  • refseq_POLR3H.R1 (reverse primer)
  • Alternative donor

Frame 0

AQARAAVRGAWCVVRDTSGGTWRGCGAWSGVAW
SGVAWRGLAARARTRSCGRRVPPSGLSFLML**R
SMARTGPPRPPRATSPPAWGPQRLGRDQGARGF
SLPPALVRD*PSCSQSPQRRPGCSSWWKWWTPS
GSPLGSLRGSSTTPLPRS*TRSWPTRSCTTWDSA
FVCLISPNWRMPMYSLGMAHHTPKSIFAAWCFI
HS
*MRFSLGRSKAAAQKECTSL*ASSMTFSSPQ
SHCS
SQPSSTKRSRCGCGSTRRRKEHTTSTWTPA
RRSASGWWTRALLTRPPQGPAQQMPPLPVRSCQ
RRRLRTRLWDPSVSQAWAFSPGGPATSPGAGQW
TLPACGKVVWPAVKTTAAEADAKEIVSRAGQ*GK
ARSAHQLAPLLHHLGPAPPSLQ*WIHLWLWLLG
LH*P*LKRPFLDPGLDNKRHRHQSLTEQSLVLD
SCFPCKNKRFSRILQPPMK*SGRRKTLDGSLVIK
NKYSCMELLKI*TKLKIGRRGLKSYFKNPPTTT
TVTPWAKRGAGSMSGHTILE*EAMAGRIGSTDG
TRGAELDASGGAGRHCPHCCSSFILLRALPARNH
SICVSLKVWFRRMVSWVPLGCLMMHFRGCKEKV
GVGGSGGYCVPLAPQGGQGDRALPGDLRGQQAN
QILGQSVPDGPGQTQVAPTGDSWGPEGPAVGGLS
PECQVLVSKGRLGRCEGPGLTPTPNSEKPLCFL
CGATWRTGGLSSWNEASWEKLPWGQCRRGNKVA
APHPTTSGGPGPLTLPGVKSFRP*MVSLSTG*IL
L*SAGSAKVRGSRPCFFRLVSGKQAAFGHRGPD
*PGELHFLNPSGALGDAPNPAAWDNARAVTMGP
WSAPFH*GCP*QVCAGAEVCEAFLLHIHDQKEPP
GTCPRSQRLRSTSLEAGPAGSREAERRMCTV*A
*EGARG*RACGLQWTQPRTRPNSATGRESDLDH
*GWDEDGLGTDSSSPWILAKLLVMMARPPRWRGS
RAACSRLEPSP*FSSPITTHLMPCFCQASGGKV
IRQRRAPSSPVWPYLPGLTHLAPGHPEAPGKGA
GGALPRLLPKGK*SLRE*DPNEAPRGQRHVVVCS
GKEDRSHPLVLGRGRCVWREVG*DRDGLRAPHK
MHL*SSRRPLPGSTLCPTQPPGGARVLSPGWAR
TWVQPWFCLFLKAEKVQSPQTLSACSL*GPV*PP
L*AFGL*LPESAPFLVFV*GQIS*DISH*LLLV
QGPGCTTGPGPHAFSATGRGCRPLLPSTAVPFG
LGRPPSQFPLP*CPPCTCLAGPFRSDHWHCPYQL
*PTL**RAVSGTGPNS*VTALRTELALPFSMLM
APMSRLLEMLSKCPRNLSQGPAAEMWSVVHFPL
TLGGK*RGQRSGHGPGGVDDKPSRGRQQAPGPRR
GDRGVRGRQVFRHVFCGPGASTLRPHGLRPGLH
PLKGLWLLSLPYGAEAAQSWDRSMFAHPSGLSC
KISHRPHAGHRSPGHAWVLADD*LVSSVTGVPGD
PLISSINKYLTQIGGLWPHQK

Frame 1

RKRERLCAVRGAWCVIRAAARGAGAGRGVAWRG
VAWRGGVSRRGRAPGAVDGECLPLASVSSCCSS
GAWPGPGPRDRPVQPHRQPGGLSDWDGTKGLGDS
PCPRPWCVTDPPVPRAPSAGRDVRPGGNGGHRP
DPPLAV*EEAQRLHCRGAEQEVGQQGRVQRGTL
HLSV*YHQTGGCLCIPWGWRITHQSPFSLRGVSS
IP
R*DSHWEDQRLQPRRSARLSRLLR*HSHPPR
VTAA
ASQVRRSGAGVGVGVRDGGRSTRPLHGHR
RGDPLPGGGRELC*HVPHRAQLSRCHHFQ*GAAK
EGGSVHACGIHQ*ARPGPSLLVDQQLALGLDSG
PYQPAGRWYGRL*RQQQLRPMLRR*CLELDSEA
RPGVPTSLPRSSTI*ALLLLHCSDGSICGCGCWA
STDLD*KDPSWTLDLTTNGTDTNHSQNSHWFWI
LVFLARTKGSLESFSHQ*NDQAGEKL*MGVLSS
KINTVAWSCLRSEPNSK*EDGG*KVILRTPPPQP
QSLRGLSGEQEACLVTPSWNRKPWLDGSDPLMA
HVELNLTPAAGRGGTALTVAVPSFC*GHCQRGT
TQSASR*RCGSGGWSPGSRWGA**CTSGAARRRW
VWVEVVGTVCPWPRRGVRETGHCLGT*EVSRQT
KS*ASLCLMAQVRPRWHLQGIPGAQRVLQWVGC
PLSARSW*VKGGWGGVRAPG*LQPPTLRSHFAFS
VGPPGELGAFPAGMKPPGRSYPGASAGGETRWL
PLTPPPQGDPGP*PCQG*SPSGPEWSACPQGES
CCSQPGQRRSEAAGPVSSGWSLESRRHSVIGGLT
SLESFIF*THLGLWEMPLILLPGTMPGQ*LWGP
GLLPFTRGAPSKCALVLRSARPSCCTFMIRKNP
LAHVLDLSGSDLQAWKQGLREAGKLSVGCALCEP
KKGQEADGPVGCSGHSLEPGPTQPRAGRVTLIT
RDGMRMAWVQTPAHRGSWQSSW**WPGPQGGEA
PELHAPGSSPRRSSRLRSPPT*CHVSVRQAGVRL
*GRGGHRPHLSGPTCLASPTWRLGILRLPVKGL
EGPFLACCLRASEACVNETQTRPRGDRGTWLFA
VAKKTEATHWCLAEAGVCGGRLGETGTASGPHTR
CICEAQGAPFLGAPSAPPSHQGVPESFHPAGPG
LGFSPGSVCFSKQKKSNHLRPSLPAHCEAPCSP
LSELLASDFLSRPPSLSLSRARYPKISLTNSSWC
RDPAVPPDLGLMPSQPQGGAAGLSSLPQQSPLG
LVGLLPSFHSPNVLPAPALQAPSGVTTGIAPIN
SDPPCSSGQCQGRGQTPE*RHCARSWPCRFQC*W
HQ*AGCWRCYPSAHGT*ARGQQLRCGQWYTSL*
PWVVSREDRGQGMALEGWMTSPPEAGSRPRGPG
GVTEG*EAGKSSDTCSVVLVLPP*GHMGSGQAFI
PSKASGCSVSLMGQRPPKVGTGQCSLTLQASAA
RSATDLMLDTGAQDMPGSWLMTD*SLLSQGCLE
TP**AA*INI*HRLEASGHTK

Frame 2

ASASGCARCVVRGA*YERRHVARVRGVEWRGVE
WRGVAGSRGAGAHPELWTESASLWPQFPHVVVA
EHGPDRAPETAPCNLTASLGASATGTGPRGSGI
LPAPGPGA*LTLLFPEPPAQAGMFVLVEMVDTVR
IPPWQFERKLNDSIAEELNKKLANKVVYNVGLC
ICLFDITKLEDAYVFPGDGASHTKVHFRCVVFH
PF
LDEILIGKIKGCSPEGVHVSLGFFDDILIPPE
SLQQ
PAKFDEAEQVWVWEYETEEGAHDLYMDTG
EEIRFRVVDESFVDTSPTGPSSADATTSSEELP
KKEAPYTLVGSISEPGLGLLSWWTSN*PWGWTVD
PTSLREGGMAGCEDNSS*GRC*GDSVSSWTVRQ
GQECPPACPAPPPSRPCSSFTAVMDPSVAVAVG
PPLTLTEKTLPGPWT*QQTAQTPITHRTVTGFGF
LFSLQEQKVL*NPSATNEMIRQEKNFRWESCHQ
K*IQLHGAA*DLNQTQNRKTGAKKLF*EPPHHN
HSHSVG*AGSRKHVWSHHLGIGSHGWTDRIH*WH
TWS*T*RQRRGGEALPSLLQFLHSVEGTASAEP
LNLRLVEGVVQEDGLLGPVGVLDDALQGLQGEG
GCGWKWWVLCALGPAGGSGRQGTAWGPKRSAGKP
NPRPVCA*WPRSDPGGTYRGFLGPRGSCSGWAV
P*VPGPGE*REVGEV*GPRVDSNPQL*EATLLS
LWGHLENWGPFQLE*SLLGEATLGPVQEGKQGGC
PSPHHLRGTRAPNLARGEVLQALNGQLVHRVNL
VVVSRVSEGQRQQALFLQVGLWKAGGIRSSGA*
LAWRASFSEPIWGSGRCP*SCCLGQCPGSDYGAL
VCSLSLGVPLASVRWC*GLRGLPAAHS*SERTP
WHMS*ISAAQIYKPGSRACGKPGS*ASDVHCVS
LRRGKRLTGLWAAVDTA*NQAQLSHGQGE*P*SL
GMG*GWPGYRLQLTVDPGKALGDDGPAPKVARL
QSCMLPARALAVVLVSDHHPPDAMFLSGKRG*G
YKAEEGTVLTCLALPAWPHPPGAWAS*GSR*RGW
RGPSSPAA*GQVKPA*MRPKRGPEGTEARGCLQ
WQRRQKPPIGAWQRQVCVEGGWVRQGRPPGPTQ
DASVKLKAPPSWEHPLPHPATRGCQSPFTRLGQD
LGSALVLFVSQSRKSPITSDPLCLLTVRPRVAP
SLSFWPLTS*VGPLPCLCLGPDILRYLSLTPPG
AGTRLYHRTWASCLLSHREGLQASPPFHSSPLWA
WSASFPVSTPLMSSLHLPCRPLQE*PLALPLST
LTHLVVAGSVRDGAKLLSDGIAHGVGLAVFNVD
GTNEQVVGDVIQVPTELEPGASS*DVVSGTLPFD
LGW*VERTEVRAWPWRGG*QALQRQAAGPGAQE
G*QRGERQASLQTRVLWSWCFHPKATWAQARPS
SPQRPLAAQSPLWGRGRPKLGQVNVRSPFRPQLQ
DQPQTSCWTQEPRTCLGPG**LISLFCHRGAWR
PLNKQHK*ISDTDWRPLATPK

Sequence NM_001018051

GCGCAAGCGCGAGCGGCTGTGCGCGGTGCGTGGTGCGTGGTGCGTGATACGAGCGGCGGCACGTGGCGCGGGTGCGGGGCGTGGAGTGGCGTGGCGTGGA
GTGGCGTGGCGTGGCGGGGTCTCGCGGCGCGGGCGCGCACCCGGAGCTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGTTTCCTCATGTTGTAGTAGCG
GAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCAACCTCACCGCCAGCCTGGGGGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTC
TCCCTGCCCCCGGCCCTGGTGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGGCCGGGATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCG
GATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTGC
ATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATC
CATTCC
TAGATGAGATTCTCATTGGGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGGTAACAGTGGCCCTGACGCCTCAGACAAGACTTGGGAACA
CTGTGGGGGCAGGAG
TCTCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTCGACGAAGCGGAGCAGGTGTG
GGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGGCGAGGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACG
TCCCCCACAGGGCCCAGCTCAGCAGATGCCACCACTTCCAGTGAGGAGCTGCCAAAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGCCAG
GCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAGCCCTGGGGCTGGACAGTGGACCCTACCAGCCTGCGGGAAGGTGGTATGGCCGGCTGTGAAGACAA
CAGCAGCTGAGGCCGATGCTAAGGAGATAGTGTCTCGAGCTGGACAGTGAGGCAAGGCCAGGAGTGCCCACCAGCTTGCCCCGCTCCTCCACCATCTAGG
CCCTGCTCCTCCTTCACTGCAGTGATGGATCCATCTGTGGCTGTGGCTGTTGGGCCTCCACTGACCTTGACTGAAAAGACCCTTCCTGGACCCTGGACTT
GACAACAAACGGCACAGACACCAATCACTCACAGAACAGTCACTGGTTTTGGATTCTTGTTTTCCTTGCAAGAACAAAAGGTTCTCTAGAATCCTTCAGC
CACCAATGAAATGATCAGGCAGGAGAAAAACTTTAGATGGGAGTCTTGTCATCAAAAATAAATACAGTTGCATGGAGCTGCTTAAGATCTGAACCAAACT
CAAAATAGGAAGACGGGGGCTAAAAAGTTATTTTAAGAACCCCCCCACCACAACCACAGTCACTCCGTGGGCTAAGCGGGGAGCAGGAAGCATGTCTGGT
CACACCATCTTGGAATAGGAAGCCATGGCTGGACGGATCGGATCCACTGATGGCACACGTGGAGCTGAACTTGACGCCAGCGGCGGGGCGGGGAGGCACT
GCCCTCACTGTTGCAGTTCCTTCATTCTGTTGAGGGCACTGCCAGCGCGGAACCACTCAATCTGCGTCTCGTTGAAGGTGTGGTTCAGGAGGATGGTCTC
CTGGGTCCCGTTGGGGTGCTTGATGATGCACTTCAGGGGCTGCAAGGAGAAGGTGGGTGTGGGTGGAAGTGGTGGGTACTGTGTGCCCTTGGCCCCGCAG
GGGGGTCAGGGAGACAGGGCACTGCCTGGGGACCTAAGAGGTCAGCAGGCAAACCAAATCCTAGGCCAGTCTGTGCCTGATGGCCCAGGTCAGACCCAGG
TGGCACCTACAGGGGATTCCTGGGGCCCAGAGGGTCCTGCAGTGGGTGGGCTGTCCCCTGAGTGCCAGGTCCTGGTGAGTAAAGGGAGGTTGGGGAGGTG
TGAGGGCCCCGGGTTGACTCCAACCCCCAACTCTGAGAAGCCACTTTGCTTTCTCTGTGGGGCCACCTGGAGAACTGGGGGCCTTTCCAGCTGGAATGAA
GCCTCCTGGGAGAAGCTACCCTGGGGCCAGTGCAGGAGGGGAAACAAGGTGGCTGCCCCTCACCCCACCACCTCAGGGGGACCCGGGCCCCTAACCTTGC
CAGGGGTGAAGTCCTTCAGGCCCTGAATGGTCAGCTTGTCCACAGGGTGAATCTTGTTGTAGTCAGCCGGGTCAGCGAAGGTCAGAGGCAGCAGGCCCTG
TTTCTTCAGGTTGGTCTCTGGAAAGCAGGCGGCATTCGGTCATCGGGGGCCTGACTAGCCTGGAGAGCTTCATTTTCTGAACCCATCTGGGGCTCTGGGA
GATGCCCCTAATCCTGCTGCCTGGGACAATGCCCGGGCAGTGACTATGGGGCCCTGGTCTGCTCCCTTTCACTAGGGGTGCCCCTAGCAAGTGTGCGCTG
GTGCTGAGGTCTGCGAGGCCTTCCTGCTGCACATTCATGATCAGAAAGAACCCCCTGGCACATGTCCTAGATCTCAGCGGCTCAGATCTACAAGCCTGGA
AGCAGGGCCTGCGGGAAGCCGGGAAGCTGAGCGTCGGATGTGCACTGTGTGAGCCTAAGAAGGGGCAAGAGGCTGACGGGCCTGTGGGCTGCAGTGGACA
CAGCCTAGAACCAGGCCCAACTCAGCCACGGGCAGGGAGAGTGACCTTGATCACTAGGGATGGGATGAGGATGGCCTGGGTACAGACTCCAGCTCACCGT
GGATCCTGGCAAAGCTCTTGGTGATGATGGCCCGGCCCCCAAGGTGGCGAGGCTCCAGAGCTGCATGCTCCCGGCTCGAGCCCTCGCCGTAGTTCTCGTC
TCCGATCACCACCCACCTGATGCCATGTTTCTGTCAGGCAAGCGGGGGTAAGGTTATAAGGCAGAGGAGGGCACCGTCCTCACCTGTCTGGCCCTACCTG
CCTGGCCTCACCCACCTGGCGCCTGGGCATCCTGAGGCTCCCGGTAAAGGGGCTGGAGGGGCCCTTCCTCGCCTGCTGCCTAAGGGCAAGTGAAGCCTGC
GTGAATGAGACCCAAACGAGGCCCCGAGGGGACAGAGGCACGTGGTTGTTTGCAGTGGCAAAGAAGACAGAAGCCACCCATTGGTGCTTGGCAGAGGCAG
GTGTGTGTGGAGGGAGGTTGGGTGAGACAGGGACGGCCTCCGGGCCCCACACAAGATGCATCTGTGAAGCTCAAGGCGCCCCCTTCCTGGGAGCACCCTC
TGCCCCACCCAGCCACCAGGGGGTGCCAGAGTCCTTTCACCCGGCTGGGCCAGGACTTGGGTTCAGCCCTGGTTCTGTTTGTTTCTCAAAGCAGAAAAAG
TCCAATCACCTCAGACCCTCTCTGCCTGCTCACTGTGAGGCCCCGTGTAGCCCCCTCTCTGAGCTTTTGGCCTCTGACTTCCTGAGTCGGCCCCCTTCCT
TGTCTTTGTCTAGGGCCAGATATCCTAAGATATCTCTCACTAACTCCTCCTGGTGCAGGGACCCGGCTGTACCACCGGACCTGGGCCTCATGCCTTCTCA
GCCACAGGGAGGGGCTGCAGGCCTCTCCTCCCTTCCACAGCAGTCCCCTTTGGGCTTGGTCGGCCTCCTTCCCAGTTTCCACTCCCCTAATGTCCTCCCT
GCACCTGCCTTGCAGGCCCCTTCAGGAGTGACCACTGGCATTGCCCCTATCAACTCTGACCCACCTTGTAGTAGCGGGCAGTGTCAGGGACGGGGCCAAA
CTCCTGAGTGACGGCATTGCGCACGGAGTTGGCCTTGCCGTTTTCAATGTTGATGGCACCAATGAGCAGGTTGTTGGAGATGTTATCCAAGTGCCCACGG
AACTTGAGCCAGGGGCCAGCAGCTGAGATGTGGTCAGTGGTACACTTCCCTTTGACCTTGGGTGGTAAGTAGAGAGGACAGAGGTCAGGGCATGGCCCTG
GAGGGGTGGATGACAAGCCCTCCAGAGGCAGGCAGCAGGCCCCGGGGCCCAGGAGGGGTGACAGAGGGGTGAGAGGCAGGCAAGTCTTCAGACACGTGTT
CTGTGGTCCTGGTGCTTCCACCCTAAGGCCACATGGGCTCAGGCCAGGCCTTCATCCCCTCAAAGGCCTCTGGCTGCTCAGTCTCCCTTATGGGGCAGAG
GCCGCCCAAAGTTGGGACAGGTCAATGTTCGCTCACCCTTCAGGCCTCAGCTGCAAGATCAGCCACAGACCTCATGCTGGACACAGGAGCCCAGGACATG
CCTGGGTCCTGGCTGATGACTGATTAGTCTCTTCTGTCACAGGGGTGCCTGGAGACCCCTTAATAAGCAGCATAAATAAATATCTGACACAGATTGGAGG
CCTCTGGCCACACCAAAAA
  • refseq_POLR3H.F1 (forward primer)
  • refseq_POLR3H.R1 (reverse primer)
  • Alternative donor

Frame 0

AQARAAVRGAWCVVRDTSGGTWRGCGAWSGVAW
SGVAWRGLAARARTRSCGRRVPPSGLSFLML**R
SMARTGPPRPPRATSPPAWGPQRLGRDQGARGF
SLPPALVRD*PSCSQSPQRRPGCSSWWKWWTPS
GSPLGSLRGSSTTPLPRS*TRSWPTRSCTTWDSA
FVCLISPNWRMPMYSLGMAHHTPKSIFAAWCFI
HS
*MRFSLGRSKAAAQKECTVTVALTPQTRLGN
TVGAG
VSLGFFDDILIPPESLQQPAKFDEAEQVW
VWEYETEEGAHDLYMDTGEEIRFRVVDESFVDT
SPTGPSSADATTSSEELPKKEAPYTLVGSISEP
GLGLLSWWTSN*PWGWTVDPTSLREGGMAGCEDN
SS*GRC*GDSVSSWTVRQGQECPPACPAPPPSR
PCSSFTAVMDPSVAVAVGPPLTLTEKTLPGPWT
*QQTAQTPITHRTVTGFGFLFSLQEQKVL*NPSA
TNEMIRQEKNFRWESCHQK*IQLHGAA*DLNQT
QNRKTGAKKLF*EPPHHNHSHSVG*AGSRKHVW
SHHLGIGSHGWTDRIH*WHTWS*T*RQRRGGEAL
PSLLQFLHSVEGTASAEPLNLRLVEGVVQEDGL
LGPVGVLDDALQGLQGEGGCGWKWWVLCALGPA
GGSGRQGTAWGPKRSAGKPNPRPVCA*WPRSDPG
GTYRGFLGPRGSCSGWAVP*VPGPGE*REVGEV
*GPRVDSNPQL*EATLLSLWGHLENWGPFQLE*
SLLGEATLGPVQEGKQGGCPSPHHLRGTRAPNLA
RGEVLQALNGQLVHRVNLVVVSRVSEGQRQQAL
FLQVGLWKAGGIRSSGA*LAWRASFSEPIWGSG
RCP*SCCLGQCPGSDYGALVCSLSLGVPLASVRW
C*GLRGLPAAHS*SERTPWHMS*ISAAQIYKPG
SRACGKPGS*ASDVHCVSLRRGKRLTGLWAAVD
TA*NQAQLSHGQGE*P*SLGMG*GWPGYRLQLTV
DPGKALGDDGPAPKVARLQSCMLPARALAVVLV
SDHHPPDAMFLSGKRG*GYKAEEGTVLTCLALP
AWPHPPGAWAS*GSR*RGWRGPSSPAA*GQVKPA
*MRPKRGPEGTEARGCLQWQRRQKPPIGAWQRQ
VCVEGGWVRQGRPPGPTQDASVKLKAPPSWEHP
LPHPATRGCQSPFTRLGQDLGSALVLFVSQSRKS
PITSDPLCLLTVRPRVAPSLSFWPLTS*VGPLP
CLCLGPDILRYLSLTPPGAGTRLYHRTWASCLL
SHREGLQASPPFHSSPLWAWSASFPVSTPLMSSL
HLPCRPLQE*PLALPLSTLTHLVVAGSVRDGAK
LLSDGIAHGVGLAVFNVDGTNEQVVGDVIQVPT
ELEPGASS*DVVSGTLPFDLGW*VERTEVRAWPW
RGG*QALQRQAAGPGAQEG*QRGERQASLQTRV
LWSWCFHPKATWAQARPSSPQRPLAAQSPLWGR
GRPKLGQVNVRSPFRPQLQDQPQTSCWTQEPRTC
LGPG**LISLFCHRGAWRPLNKQHK*ISDTDWR
PLATPK

Frame 1

RKRERLCAVRGAWCVIRAAARGAGAGRGVAWRG
VAWRGGVSRRGRAPGAVDGECLPLASVSSCCSS
GAWPGPGPRDRPVQPHRQPGGLSDWDGTKGLGDS
PCPRPWCVTDPPVPRAPSAGRDVRPGGNGGHRP
DPPLAV*EEAQRLHCRGAEQEVGQQGRVQRGTL
HLSV*YHQTGGCLCIPWGWRITHQSPFSLRGVSS
IP
R*DSHWEDQRLQPRRSAR*QWP*RLRQDLGT
LWGQE
SL*ASSMTFSSPQSHCSSQPSSTKRSRC
GCGSTRRRKEHTTSTWTPARRSASGWWTRALLTR
PPQGPAQQMPPLPVRSCQRRRLRTRLWDPSVSQ
AWAFSPGGPATSPGAGQWTLPACGKVVWPAVKT
TAAEADAKEIVSRAGQ*GKARSAHQLAPLLHHLG
PAPPSLQ*WIHLWLWLLGLH*P*LKRPFLDPGL
DNKRHRHQSLTEQSLVLDSCFPCKNKRFSRILQ
PPMK*SGRRKTLDGSLVIKNKYSCMELLKI*TKL
KIGRRGLKSYFKNPPTTTTVTPWAKRGAGSMSG
HTILE*EAMAGRIGSTDGTRGAELDASGGAGRH
CPHCCSSFILLRALPARNHSICVSLKVWFRRMVS
WVPLGCLMMHFRGCKEKVGVGGSGGYCVPLAPQ
GGQGDRALPGDLRGQQANQILGQSVPDGPGQTQ
VAPTGDSWGPEGPAVGGLSPECQVLVSKGRLGRC
EGPGLTPTPNSEKPLCFLCGATWRTGGLSSWNE
ASWEKLPWGQCRRGNKVAAPHPTTSGGPGPLTL
PGVKSFRP*MVSLSTG*ILL*SAGSAKVRGSRPC
FFRLVSGKQAAFGHRGPD*PGELHFLNPSGALG
DAPNPAAWDNARAVTMGPWSAPFH*GCP*QVCA
GAEVCEAFLLHIHDQKEPPGTCPRSQRLRSTSLE
AGPAGSREAERRMCTV*A*EGARG*RACGLQWT
QPRTRPNSATGRESDLDH*GWDEDGLGTDSSSP
WILAKLLVMMARPPRWRGSRAACSRLEPSP*FSS
PITTHLMPCFCQASGGKVIRQRRAPSSPVWPYL
PGLTHLAPGHPEAPGKGAGGALPRLLPKGK*SL
RE*DPNEAPRGQRHVVVCSGKEDRSHPLVLGRGR
CVWREVG*DRDGLRAPHKMHL*SSRRPLPGSTL
CPTQPPGGARVLSPGWARTWVQPWFCLFLKAEK
VQSPQTLSACSL*GPV*PPL*AFGL*LPESAPFL
VFV*GQIS*DISH*LLLVQGPGCTTGPGPHAFS
ATGRGCRPLLPSTAVPFGLGRPPSQFPLP*CPP
CTCLAGPFRSDHWHCPYQL*PTL**RAVSGTGPN
S*VTALRTELALPFSMLMAPMSRLLEMLSKCPR
NLSQGPAAEMWSVVHFPLTLGGK*RGQRSGHGP
GGVDDKPSRGRQQAPGPRRGDRGVRGRQVFRHVF
CGPGASTLRPHGLRPGLHPLKGLWLLSLPYGAE
AAQSWDRSMFAHPSGLSCKISHRPHAGHRSPGH
AWVLADD*LVSSVTGVPGDPLISSINKYLTQIGG
LWPHQK

Frame 2

ASASGCARCVVRGA*YERRHVARVRGVEWRGVE
WRGVAGSRGAGAHPELWTESASLWPQFPHVVVA
EHGPDRAPETAPCNLTASLGASATGTGPRGSGI
LPAPGPGA*LTLLFPEPPAQAGMFVLVEMVDTVR
IPPWQFERKLNDSIAEELNKKLANKVVYNVGLC
ICLFDITKLEDAYVFPGDGASHTKVHFRCVVFH
PF
LDEILIGKIKGCSPEGVHGNSGPDASDKTWEH
CGGRS
LSRLLR*HSHPPRVTAAASQVRRSGAGV
GVGVRDGGRSTRPLHGHRRGDPLPGGGRELC*H
VPHRAQLSRCHHFQ*GAAKEGGSVHACGIHQ*AR
PGPSLLVDQQLALGLDSGPYQPAGRWYGRL*RQ
QQLRPMLRR*CLELDSEARPGVPTSLPRSSTI*
ALLLLHCSDGSICGCGCWASTDLD*KDPSWTLDL
TTNGTDTNHSQNSHWFWILVFLARTKGSLESFS
HQ*NDQAGEKL*MGVLSSKINTVAWSCLRSEPN
SK*EDGG*KVILRTPPPQPQSLRGLSGEQEACLV
TPSWNRKPWLDGSDPLMAHVELNLTPAAGRGGT
ALTVAVPSFC*GHCQRGTTQSASR*RCGSGGWS
PGSRWGA**CTSGAARRRWVWVEVVGTVCPWPRR
GVRETGHCLGT*EVSRQTKS*ASLCLMAQVRPR
WHLQGIPGAQRVLQWVGCPLSARSW*VKGGWGG
VRAPG*LQPPTLRSHFAFSVGPPGELGAFPAGMK
PPGRSYPGASAGGETRWLPLTPPPQGDPGP*PC
QG*SPSGPEWSACPQGESCCSQPGQRRSEAAGP
VSSGWSLESRRHSVIGGLTSLESFIF*THLGLWE
MPLILLPGTMPGQ*LWGPGLLPFTRGAPSKCAL
VLRSARPSCCTFMIRKNPLAHVLDLSGSDLQAW
KQGLREAGKLSVGCALCEPKKGQEADGPVGCSGH
SLEPGPTQPRAGRVTLITRDGMRMAWVQTPAHR
GSWQSSW**WPGPQGGEAPELHAPGSSPRRSSR
LRSPPT*CHVSVRQAGVRL*GRGGHRPHLSGPTC
LASPTWRLGILRLPVKGLEGPFLACCLRASEAC
VNETQTRPRGDRGTWLFAVAKKTEATHWCLAEA
GVCGGRLGETGTASGPHTRCICEAQGAPFLGAPS
APPSHQGVPESFHPAGPGLGFSPGSVCFSKQKK
SNHLRPSLPAHCEAPCSPLSELLASDFLSRPPS
LSLSRARYPKISLTNSSWCRDPAVPPDLGLMPSQ
PQGGAAGLSSLPQQSPLGLVGLLPSFHSPNVLP
APALQAPSGVTTGIAPINSDPPCSSGQCQGRGQ
TPE*RHCARSWPCRFQC*WHQ*AGCWRCYPSAHG
T*ARGQQLRCGQWYTSL*PWVVSREDRGQGMAL
EGWMTSPPEAGSRPRGPGGVTEG*EAGKSSDTC
SVVLVLPP*GHMGSGQAFIPSKASGCSVSLMGQR
PPKVGTGQCSLTLQASAARSATDLMLDTGAQDM
PGSWLMTD*SLLSQGCLETP**AA*INI*HRLE
ASGHTK

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