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Sequences

Sequence NM_177990

CAGTAGTAGTTCCCCAGCGTGCGCCCGGGGAGACCGGGAACATGGCGCTGGGAGCGCTGTAGCAGCTGAGAAGGGGCTGAGGCACCGCCGCTTCGCTGAC AGCCGGCCACCAGAAATACACTGTGGTGAAATGCTTCCACCTCTTGCTAAAATGAACACTGAGGAAAAATGAAGAAGACTGACAAGCACCAGCGAAAAGT TGCAGAATAGAAATAGCCACACTCCTCTGGAGTCTTTAATTCATCCACAGCCATCATATAAAGGTTTTGGCATCATGTTTGGGAAGAAAAAGAAAAAGAT TGAAATATCTGGCCCGTCCAACTTTGAACACAGGGTTCATACTGGGTTTGATCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCACAGCCTG TTAGCAGATACGGCCAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATCACACCCATCCAGCTGGCTCCTATGAAGACAATCGTTAGAGGAAACAAAC CCTGCAAGGAAACCTCCATCAACGGCCTGCTAGAGGATTTTGACAACATCTCGGTGACTCGCTCCAACTCCCTAAGGAAAGAAAGCCCACCCACCCCAGA TCAGGGAGCCTCCAGCCACGGTCCAGGCCACGCGGAAGAAAATGGCTTCATCACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGACTACACG ACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTATTATAGAGGCAGCCACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGA AGCACGGGGAGGCCTACTATTCTGAGGTGAAGCCTTTGAAATCCGATTTTGCCAGATTTTCTGCCGATTATCACTCACATTTGGACTCACTGAGCAAACC AAGTGAATACAGTGACCTCAAGTGGGAGTATCAGAGAGCCTCGAGTAGCTCCCCTCTGGATTATTCATTCCAATTCACACCTTCTAGAACTGCAGGGACC AGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATGGGGACCCAGCCTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGA CAAGCCCTCAGCCCACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCCAGGAACCGATGATGCCATTTGGAGCAAGTGCATTTAAAACCCATCCCCA AGGACACTCCTACAACTCCTACACCTACCCTCGCTTGTCCGAGCCCACAATGTGCATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTCAGCCCT CCACTGTCAGGGTCTGACACCTACCCCAGGGGCCCTGCCAAACTACCTCAAAGTCAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACCCGTCTG GGTACCACAAAGCCACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGCAGTACATCTCCACGGCTTCCTACCTGAGCTCCCTCAGCCTCTCATCCAG CACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCCGACCAGCAGCCCTCCAGGGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTCAGCCCA GGAGACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCAACCGGCATCGTATGCATCGCCACCGAGAAACACACAGGGAAACAAGTTG CAGTGAAGAAAATGGACCTCCGGAAGCAACAGAGACGAGAACTGCTTTTCAATGAGGTCGTGATCATGCGGGATTACCACCATGACAATGTGGTTGACAT GTACAGCAGCTACCTTGTCGGCGATGAGCTCTGGGTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTGACTCACACCAGAATGAATGAA GAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTTCATAACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCC TGACAAGCGATGGCCGGATAAAGTTGTCTGATTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCGAAGAGGAAATCATTGGTTGGCACTCCCTACTG GATGGCCCCTGAGGTGATTTCTAGGCTACCTTATGGGACAGAGGTGGACATCTGGTCCCTCGGGATCATGGTGATAGAAATGATTGATGGCGAGCCCCCC TACTTCAATGAGCCTCCCCTCCAGGCGATGCGGAGGATCCGGGACAGTTTACCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGAT TCCTAGACTTGATGTTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGGAACTCCTCGGACATCCATTCTTAAAACTAGCAGGTCCACCGTCTTGCAT CGTCCCCCTCATGAGACAATACAGGCATCACTGAGCAGAGGATTCGTGTAGGTGGCAAAGCTAGATGAGGACATGAGAATAATTCAGGAGAACAAAAGGA AACACAGAACATGCAAAAGGCCTGTGCATTCTAGACCAGCCAATTGGTGGGACAGCGTGATGACCGGCAGGGTTCAACAGACCAGGGCATCTTCTTGTGT CTTAAACAGGCATCTCTCCACTGACAGCCGGTGTGGTCACTTGGAGCACGGCTTTAATAAGTCATTATTATATTTTTCAGCCCTTCATCCAGCAAATCAG AAGGACTCAGTACAAACTCCGTTATGATATATCCTAGCCACATGCAGGGTAACATGTAGGATTTTCTATATTGAAAGAATACTTTTCTGGCAAAAAAAAA AAAAAAAGAAAGAAAGGAAAACAAAAAGCACTTTTTTCTTAATGGTAGCAGTATAATGTATTTTGCAACGAATTTGTAATTTTTCTGTACGATAGTTTTG ATAATTTATAGTACTTTGATGTCATGTAGCCATTGTATCAGTTGAAGTAATACTTGTTTACTAGAGGAGTTTGAACAAAGCCTTTCCTACTTTTTTATCC CTTTAAGAGAACCAATGATTCTTTAGGAACTTTGAATACTGAATGACTCTCAATCACCGTCAGCTTTAGTAAAATCTCTTTCTTATCCTAACAAGTGTCT TATTTGGTGGAAGAAGAATTAAGAGTGATGGTGATGGTGTGCACGTTTCATTAATCCAACCAAAAATAATGAAATAAAATTTGAGCCACAGTATACCACT CCTTGGGATAAAGTTAAATATTTTTAAAGATCACATTTTCCATGAACGCCTCTAGTAGCAAACCATTCTTTTGCACACCACAATGTTTCCCTCAGTGCCC TTTCTCAAATGGGTACAATGTTCCCTTGTGGCCAAATTTCCCTCCCAGGGAGCAATTTCAGTGCTAGGATCATTGGATTCAGTTCCCAAAATAGAATGTT TCAGTGAGACCATGAGAATTCCAGGCTCACAAAGGGAGAGGAGAGAACAGGGCAAGACGTTTGGTTTCATTTGTCACCATTTTTAAAACTCTGTATGCTA GCACACCAAACTCTTGTCTATATTTACCTTTGTACCACAGTATTAATCGCTATTGTTCATGTATCGTGCTGGAAGTCTGAACTGACTCTAGAGGATGAAT TAGCAAGAGGGTATTTTACCAGGTATGATCTGACTTCAGTTGTGCCCATGTTATAATGTGTTTCCGACATAGGAGAGTCGTGCTGCTGTCTAGATCTTCT TGAATGTTGATAAAAATGAATGACTACTACAATACATTTTGTGTTGCTTGTTGGATGAATTTGCATGTTAACTGTAGGCCAATATAGATTTGCCTTTAAA ACTCTGGAAGAGCTACATAGTCATCATTAGTTTCTATTAATTATGCATCAGACAAAAGCCATTTGTTACCAAACTGGGAAAACAGAGGCTTTTCTTAACT ATTTCACATACTGTAACAAATATGAATTTAAATTTGTGATAGCGCTCTGGTTGCTCTAAGCATAATTAAGAATTTTTGTAATTAATAGGTTGCTAATTAT TTATCACTGCTAAAAAGGAAAAAAGGCATAAAATGACCTTCTACTGATTAGATTTTCAGTTTTCTTTCAAACTGGAAATGCCTCCATAAATATGATCTAT GATTTTGCTTCATAAAACAGCAAATCAATGTTTTATGTAAAATATTAAAGCATTAATATAAATATGTGAGAATAAAAACAATCTAAATCCAGAAAATGGC AGTCCTAAATGTTCATGAGACAGATTGTATTAATTTAACCAGGACTATGTAGAAGTAGAAAGAAAAGAAAAAGAAAATCTTTTTTAAACCAGAATAAACA TTAAAAACTATTGCAGAAAATAGTGGATTTTGGATTCCAAACATTTTCGACAGTGTAATGGAAATTTTTCTGTAATTTTCTTACCATCGGGTATTTTTTA AAGTATTCATTGAGTTTACCAAAAGTTACTGTAGCTTAAAAGGTTTTGTGAGCACTAACTATTGGCAGAAACTGCATTTGCAAATAAAAATAAATGTTTG CCTTTT

refseq_PAK7.F1 (forward primer)
refseq_PAK7.R1 (reverse primer)
Single exon skipping

Frame 0

Q**FPSVRPGRPGTWRWERCSS*EGAEAPPLR* QPATRNTLW*NASTSC*NEH*GKMKKTDKHQRKV AE*K*PHSSGVFNSSTAII*RFWHHVWEEKEKD *NIWPVQL*TQGSYWV*STRAEVYRPSPAVAQP VSRYGQQAKAYGGPFMHHTHPAGSYEDNR*RKQT LQGNLHQRPARGF*QHLGDSLQLPKERKPTHPR SGSLQPRSRPRGRKWLHHLLPVFQRIRYYC*LH DRKVQGEESLWR*SGSVL*RQPRSQAKWARNENE ARGGLLF*GEAFEIRFCQIFCRLSLTFGLTEQT K*IQ*PQVGVSESLE*LPSGLFIPIHTF*NCRD QRVLQGEPGVQ*K*MGTQPG*L*QEAKVFVPESD KPSAHHAAEVQVRLGTPGTDDAIWSKCI*NPSP RTLLQLLHLPSLVRAHNVHSKGGLRSSTDGPQP STVRV*HLPQGPCQTTSKSKQIGLFLKQSPVPVW VPQSHLVPSPLPAEQFAVHLHGFLPELPQPLIQ HLPAAQLGLLLRPAALQGVP*TVSGGPAAGGQP RRPQGILGQLYQNRGRLNRHRMHRHRETHRETSC SEENGPPEATETRTAFQ*GRDHAGLPP*QCG*H VQQLPCRR*ALGGHGVSRRWCLDRHCDSHQNE* RTDSYCLPVSSESSLLPS*PRSDSQGHKK*LHPP DKRWPDKVV*FWFLCSSFQRGAEEEIIGWHSLL DGP*GDF*ATLWDRGGHLVPRDHGDRND*WRAP LLQ*ASPPGDAEDPGQFTSKSEGPTQGFFSAPGI PRLDVGEGALSESNSPGTPRTSILKTSRSTVLH RPPHETIQASLSRGFV*VAKLDEDMRIIQENKR KHRTCKRPVHSRPANWWDSVMTGRVQQTRASSCV LNRHLSTDSRCGHLEHGFNKSLLYFSALHPANQ KDSVQTPL*YILATCRVTCRIFYIERILFWQKK KKKKERKTKSTFFLMVAV*CILQRICNFSVR*F* *FIVL*CHVAIVSVEVILVY*RSLNKAFPTFLS L*ENQ*FFRNFEY*MTLNHRQL**NLFLILTSV LFGGRRIKSDGDGVHVSLIQPKIMK*NLSHSIPL LGIKLNIFKDHIFHERL**QTILLHTTMFPSVP FLKWVQCSLVAKFPSQGAISVLGSLDSVPKIEC FSETMRIPGSQRERREQGKTFGFICHHF*NSVC* HTKLLSIFTFVPQY*SLLFMYRAGSLN*L*RMN *QEGILPGMI*LQLCPCYNVFPT*ESRAAV*IF LNVDKNE*LLQYILCCLLDEFAC*L*ANIDLPLK LWKSYIVIISFY*LCIRQKPFVTKLGKQRLFLT ISHTVTNMNLNL**RSGCSKHN*EFL*LIGC*L FITAKKEKRHKMTFY*LDFQFSFKLEMPP*I*SM ILLHKTANQCFM*NIKALI*ICENKNNLNPENG SPKCS*DRLY*FNQDYVEVERKEKENLF*TRIN IKNYCRK*WILDSKHFRQCNGNFSVIFLPSGIF* SIH*VYQKLL*LKRFCEH*LLAETAFANKNKCL PF

Frame 1

SSSSPACARGDREHGAGSAVAAEKGLRHRRFAD SRPPEIHCGEMLPPLAKMNTEEK*RRLTSTSEK LQNRNSHTPLESLIHPQPSYKGFGIMFGKKKKKI EISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSL LADTANRPKPMVDPSCITPIQLAPMKTIVRGNK PCKETSINGLLEDFDNISVTRSNSLRKESPPTPD QGASSHGPGHAEENGFITFSQYSSESDTTADYT TEKYREKSLYGDDLDPYYRGSHAAKQNGHVMKM KHGEAYYSEVKPLKSDFARFSADYHSHLDSLSKP SEYSDLKWEYQRASSSSPLDYSFQFTPSRTAGT SGCSKESLAYSESEWGPSLDDYDRRPKSSYLNQ TSPQPTMRQRSRSGSGLQEPMMPFGASAFKTHPQ GHSYNSYTYPRLSEPTMCIPKVDYDRAQMVLSP PLSGSDTYPRGPAKLPQSQSKSGYSSSSHQYPS GYHKATLYHHPSLQSSSQYISTASYLSSLSLSSS TYPPPSWGSSSDQQPSRVSHEQFRAALQLVVSP GDPREYLANFIKIGEGSTGIVCIATEKHTGKQV AVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVDM YSSYLVGDELWVVMEFLEGGALTDIVTHTRMNE EQIATVCLSVLRALSYLHNQGVIHRDIKSDSIL LTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYW MAPEVISRLPYGTEVDIWSLGIMVIEMIDGEPP YFNEPPLQAMRRIRDSLPPRVKDLHKVSSVLRG FLDLMLVREPSQRATAQELLGHPFLKLAGPPSCI VPLMRQYRHH*AEDSCRWQS*MRT*E*FRRTKG NTEHAKGLCILDQPIGGTA**PAGFNRPGHLLV S*TGISPLTAGVVTWSTALISHYYIFQPFIQQIR RTQYKLRYDIS*PHAG*HVGFSILKEYFSGKKK KKRKKGKQKALFS*W*QYNVFCNEFVIFLYDSF DNL*YFDVM*PLYQLK*YLFTRGV*TKPFLLFYP FKRTNDSLGTLNTE*LSITVSFSKISFLS*QVS YLVEEELRVMVMVCTFH*SNQK**NKI*ATVYH SLG*S*IFLKITFSMNASSSKPFFCTPQCFPQCP FSNGYNVPLWPNFPPREQFQC*DHWIQFPK*NV SVRP*EFQAHKGRGENRARRLVSFVTIFKTLYA STPNSCLYLPLYHSINRYCSCIVLEV*TDSRG*I SKRVFYQV*SDFSCAHVIMCFRHRRVVLLSRSS *MLIKMNDYYNTFCVACWMNLHVNCRPI*ICL* NSGRAT*SSLVSINYASDKSHLLPNWENRGFS*L FHIL*QI*I*ICDSALVALSIIKNFCN**VANY LSLLKRKKGIK*PSTD*IFSFLSNWKCLHKYDL *FCFIKQQINVLCKILKH*YKYVRIKTI*IQKMA VLNVHETDCINLTRTM*K*KEKKKKIFFKPE*T LKTIAENSGFWIPNIFDSVMEIFL*FSYHRVFF KVFIEFTKSYCSLKGFVSTNYWQKLHLQIKINVC L

Frame 2

VVVPQRAPGETGNMALGAL*QLRRG*GTAASLT AGHQKYTVVKCFHLLLK*TLRKNEED*QAPAKS CRIEIATLLWSL*FIHSHHIKVLASCLGRKRKR LKYLARPTLNTGFILGLIHKSRSLPAFPSSGTAC *QIRPTGQSLWWTLHASHPSSWLL*RQSLEETN PARKPPSTAC*RILTTSR*LAPTP*GKKAHPPQ IREPPATVQATRKKMASSPSPSIPANPILLLTTR PKSTGRRVSMEMIWIRIIEAATQPSKMGT**K* STGRPTILR*SL*NPILPDFLPIITHIWTH*AN QVNTVTSSGSIREPRVAPLWIIHSNSHLLELQGP AGAPRRAWRTVKVNGDPAWMTMTGGQSLRT*IR QALSPPCGRGPGQARDSRNR*CHLEQVHLKPIP KDTPTTPTPTLACPSPQCAFQRWITIEHRWSSAL HCQGLTPTPGALPNYLKVKANRAIPQAVTSTRL GTTKPPCTITPPCRAVRSTSPRLPT*APSASHP APTRRPAGAPPPTSSPPGCPMNSFGRPCSWWSAQ ETPGNTWPTLSKSGKAQPASYASPPRNTQGNKL Q*RKWTSGSNRDENCFSMRS*SCGITTMTMWLT CTAATLSAMSSGWSWSF*KVVP*QTL*LTPE*MK NR*LLSACQF*ELSPTFITKE*FTGT*KVTPSS *QAMAG*SCLILVSVLKFPKRCRRGNHWLALPT GWPLR*FLGYLMGQRWTSGPSGSW**K*LMASPP TSMSLPSRRCGGSGTVYLQE*RTYTRFLQCSGD S*T*CW*GSPLREQQPRNSSDIHS*N*QVHRLA SSPS*DNTGITEQRIRVGGKAR*GHENNSGEQKE TQNMQKACAF*TSQLVGQRDDRQGSTDQGIFLC LKQASLH*QPVWSLGARL**VIIIFFSPSSSKS EGLSTNSVMIYPSHMQGNM*DFLY*KNTFLAKKK KKERKENKKHFFLNGSSIMYFATNL*FFCTIVL IIYSTLMSCSHCIS*SNTCLLEEFEQSLSYFFI PLREPMIL*EL*ILNDSQSPSALVKSLSYPNKCL IWWKKN*E*W*WCARFINPTKNNEIKFEPQYTT PWDKVKYF*RSHFP*TPLVANHSFAHHNVSLSA LSQMGTMFPCGQISLPGSNFSARIIGFSSQNRMF Q*DHENSRLTKGEERTGQDVWFHLSPFLKLCML AHQTLVYIYLCTTVLIAIVHVSCWKSELTLEDE LARGYFTRYDLTSVVPML*CVSDIGESCCCLDLL EC**K*MTTTIHFVLLVG*ICMLTVGQYRFAFK TLEELHSHH*FLLIMHQTKAICYQTGKTEAFLN YFTYCNKYEFKFVIALWLL*A*LRIFVINRLLII YHC*KGKKA*NDLLLIRFSVFFQTGNASINMIY DFAS*NSKSMFYVKY*SININM*E*KQSKSRKW QS*MFMRQIVLI*PGLCRSRKKRKRKSFLNQNKH *KLLQKIVDFGFQTFSTV*WKFFCNFLTIGYFL KYSLSLPKVTVA*KVL*ALTIGRNCICK*K*MF AF

Sequence NM_020341

CAGTAGTAGTTCCCCAGCGTGCGCCCGGGGAGACCGGGAACATGGCGCTGGGAGCGCTGTAGCAGCTGAGAAGGGGCTGAGGCACCGCCGCTTCGCTGAC AGCCGGCCACCAGGTTTCTGTCTCCCAGTATAATCATATCCAGCCCCATGGCTCTTACTGGGGCCTCTGAAATGTTTATCACCAAATCTGTGTCTGCAGT TCCAACCTCTTCCCTGAGCATCAAAGCTGTATTTCCAACTTTCGCTGGATGCTTCTATCTGGAAATACACTGTGGTGAAATGCTTCCACCTCTTGCTAAA ATGAACACTGAGGAAAAATGAAGAAGACTGACAAGCACCAGCGAAAAGTTGCAGAATAGAAATAGCCACACTCCTCTGGAGTCTTTAATTCATCCACAGC CATCATATAAAGGTTTTGGCATCATGTTTGGGAAGAAAAAGAAAAAGATTGAAATATCTGGCCCGTCCAACTTTGAACACAGGGTTCATACTGGGTTTGA TCCACAAGAGCAGAAGTTTACCGGCCTTCCCCAGCAGTGGCACAGCCTGTTAGCAGATACGGCCAACAGGCCAAAGCCTATGGTGGACCCTTCATGCATC ACACCCATCCAGCTGGCTCCTATGAAGACAATCGTTAGAGGAAACAAACCCTGCAAGGAAACCTCCATCAACGGCCTGCTAGAGGATTTTGACAACATCT CGGTGACTCGCTCCAACTCCCTAAGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCCTCCAGCCACGGTCCAGGCCACGCGGAAGAAAATGGCTTCAT CACCTTCTCCCAGTATTCCAGCGAATCCGATACTACTGCTGACTACACGACCGAAAAGTACAGGGAGAAGAGTCTCTATGGAGATGATCTGGATCCGTAT TATAGAGGCAGCCACGCAGCCAAGCAAAATGGGCACGTAATGAAAATGAAGCACGGGGAGGCCTACTATTCTGAGGTGAAGCCTTTGAAATCCGATTTTG CCAGATTTTCTGCCGATTATCACTCACATTTGGACTCACTGAGCAAACCAAGTGAATACAGTGACCTCAAGTGGGAGTATCAGAGAGCCTCGAGTAGCTC CCCTCTGGATTATTCATTCCAATTCACACCTTCTAGAACTGCAGGGACCAGCGGGTGCTCCAAGGAGAGCCTGGCGTACAGTGAAAGTGAATGGGGACCC AGCCTGGATGACTATGACAGGAGGCCAAAGTCTTCGTACCTGAATCAGACAAGCCCTCAGCCCACCATGCGGCAGAGGTCCAGGTCAGGCTCGGGACTCC AGGAACCGATGATGCCATTTGGAGCAAGTGCATTTAAAACCCATCCCCAAGGACACTCCTACAACTCCTACACCTACCCTCGCTTGTCCGAGCCCACAAT GTGCATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCCTCAGCCCTCCACTGTCAGGGTCTGACACCTACCCCAGGGGCCCTGCCAAACTACCTCAA AGTCAAAGCAAATCGGGCTATTCCTCAAGCAGTCACCAGTACCCGTCTGGGTACCACAAAGCCACCTTGTACCATCACCCCTCCCTGCAGAGCAGTTCGC AGTACATCTCCACGGCTTCCTACCTGAGCTCCCTCAGCCTCTCATCCAGCACCTACCCGCCGCCCAGCTGGGGCTCCTCCTCCGACCAGCAGCCCTCCAG GGTGTCCCATGAACAGTTTCGGGCGGCCCTGCAGCTGGTGGTCAGCCCAGGAGACCCCAGGGAATACTTGGCCAACTTTATCAAAATCGGGGAAGGCTCA ACCGGCATCGTATGCATCGCCACCGAGAAACACACAGGGAAACAAGTTGCAGTGAAGAAAATGGACCTCCGGAAGCAACAGAGACGAGAACTGCTTTTCA ATGAGGTCGTGATCATGCGGGATTACCACCATGACAATGTGGTTGACATGTACAGCAGCTACCTTGTCGGCGATGAGCTCTGGGTGGTCATGGAGTTTCT AGAAGGTGGTGCCTTGACAGACATTGTGACTCACACCAGAATGAATGAAGAACAGATAGCTACTGTCTGCCTGTCAGTTCTGAGAGCTCTCTCCTACCTT CATAACCAAGGAGTGATTCACAGGGACATAAAAAGTGACTCCATCCTCCTGACAAGCGATGGCCGGATAAAGTTGTCTGATTTTGGTTTCTGTGCTCAAG TTTCCAAAGAGGTGCCGAAGAGGAAATCATTGGTTGGCACTCCCTACTGGATGGCCCCTGAGGTGATTTCTAGGCTACCTTATGGGACAGAGGTGGACAT CTGGTCCCTCGGGATCATGGTGATAGAAATGATTGATGGCGAGCCCCCCTACTTCAATGAGCCTCCCCTCCAGGCGATGCGGAGGATCCGGGACAGTTTA CCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCAGTGCTCCGGGGATTCCTAGACTTGATGTTGGTGAGGGAGCCCTCTCAGAGAGCAACAGCCCAGG AACTCCTCGGACATCCATTCTTAAAACTAGCAGGTCCACCGTCTTGCATCGTCCCCCTCATGAGACAATACAGGCATCACTGAGCAGAGGATTCGTGTAG GTGGCAAAGCTAGATGAGGACATGAGAATAATTCAGGAGAACAAAAGGAAACACAGAACATGCAAAAGGCCTGTGCATTCTAGACCAGCCAATTGGTGGG ACAGCGTGATGACCGGCAGGGTTCAACAGACCAGGGCATCTTCTTGTGTCTTAAACAGGCATCTCTCCACTGACAGCCGGTGTGGTCACTTGGAGCACGG CTTTAATAAGTCATTATTATATTTTTCAGCCCTTCATCCAGCAAATCAGAAGGACTCAGTACAAACTCCGTTATGATATATCCTAGCCACATGCAGGGTA ACATGTAGGATTTTCTATATTGAAAGAATACTTTTCTGGCAAAAAAAAAAAAAAAAGAAAGAAAGGAAAACAAAAAGCACTTTTTTCTTAATGGTAGCAG TATAATGTATTTTGCAACGAATTTGTAATTTTTCTGTACGATAGTTTTGATAATTTATAGTACTTTGATGTCATGTAGCCATTGTATCAGTTGAAGTAAT ACTTGTTTACTAGAGGAGTTTGAACAAAGCCTTTCCTACTTTTTTATCCCTTTAAGAGAACCAATGATTCTTTAGGAACTTTGAATACTGAATGACTCTC AATCACCGTCAGCTTTAGTAAAATCTCTTTCTTATCCTAACAAGTGTCTTATTTGGTGGAAGAAGAATTAAGAGTGATGGTGATGGTGTGCACGTTTCAT TAATCCAACCAAAAATAATGAAATAAAATTTGAGCCACAGTATACCACTCCTTGGGATAAAGTTAAATATTTTTAAAGATCACATTTTCCATGAACGCCT CTAGTAGCAAACCATTCTTTTGCACACCACAATGTTTCCCTCAGTGCCCTTTCTCAAATGGGTACAATGTTCCCTTGTGGCCAAATTTCCCTCCCAGGGA GCAATTTCAGTGCTAGGATCATTGGATTCAGTTCCCAAAATAGAATGTTTCAGTGAGACCATGAGAATTCCAGGCTCACAAAGGGAGAGGAGAGAACAGG GCAAGACGTTTGGTTTCATTTGTCACCATTTTTAAAACTCTGTATGCTAGCACACCAAACTCTTGTCTATATTTACCTTTGTACCACAGTATTAATCGCT ATTGTTCATGTATCGTGCTGGAAGTCTGAACTGACTCTAGAGGATGAATTAGCAAGAGGGTATTTTACCAGGTATGATCTGACTTCAGTTGTGCCCATGT TATAATGTGTTTCCGACATAGGAGAGTCGTGCTGCTGTCTAGATCTTCTTGAATGTTGATAAAAATGAATGACTACTACAATACATTTTGTGTTGCTTGT TGGATGAATTTGCATGTTAACTGTAGGCCAATATAGATTTGCCTTTAAAACTCTGGAAGAGCTACATAGTCATCATTAGTTTCTATTAATTATGCATCAG ACAAAAGCCATTTGTTACCAAACTGGGAAAACAGAGGCTTTTCTTAACTATTTCACATACTGTAACAAATATGAATTTAAATTTGTGATAGCGCTCTGGT TGCTCTAAGCATAATTAAGAATTTTTGTAATTAATAGGTTGCTAATTATTTATCACTGCTAAAAAGGAAAAAAGGCATAAAATGACCTTCTACTGATTAG ATTTTCAGTTTTCTTTCAAACTGGAAATGCCTCCATAAATATGATCTATGATTTTGCTTCATAAAACAGCAAATCAATGTTTTATGTAAAATATTAAAGC ATTAATATAAATATGTGAGAATAAAAACAATCTAAATCCAGAAAATGGCAGTCCTAAATGTTCATGAGACAGATTGTATTAATTTAACCAGGACTATGTA GAAGTAGAAAGAAAAGAAAAAGAAAATCTTTTTTAAACCAGAATAAACATTAAAAACTATTGCAGAAAATAGTGGATTTTGGATTCCAAACATTTTCGAC AGTGTAATGGAAATTTTTCTGTAATTTTCTTACCATCGGGTATTTTTTAAAGTATTCATTGAGTTTACCAAAAGTTACTGTAGCTTAAAAGGTTTTGTGA GCACTAACTATTGGCAGAAACTGCATTTGCAAATAAAAATAAATGTTTGCCTTTT

refseq_PAK7.F1 (forward primer)
refseq_PAK7.R1 (reverse primer)
Single exon skipping

Frame 0

Q**FPSVRPGRPGTWRWERCSS*EGAEAPPLR* QPATRFLSPSIIISSPMALTGASEMFITKSVSAV PTSSLSIKAVFPTFAGCFYLEIHCGEMLPPLAK MNTEEK*RRLTSTSEKLQNRNSHTPLESLIHPQ PSYKGFGIMFGKKKKKIEISGPSNFEHRVHTGFD PQEQKFTGLPQQWHSLLADTANRPKPMVDPSCI TPIQLAPMKTIVRGNKPCKETSINGLLEDFDNI SVTRSNSLRKESPPTPDQGASSHGPGHAEENGFI TFSQYSSESDTTADYTTEKYREKSLYGDDLDPY YRGSHAAKQNGHVMKMKHGEAYYSEVKPLKSDF ARFSADYHSHLDSLSKPSEYSDLKWEYQRASSSS PLDYSFQFTPSRTAGTSGCSKESLAYSESEWGP SLDDYDRRPKSSYLNQTSPQPTMRQRSRSGSGL QEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTM CIPKVDYDRAQMVLSPPLSGSDTYPRGPAKLPQ SQSKSGYSSSSHQYPSGYHKATLYHHPSLQSSS QYISTASYLSSLSLSSSTYPPPSWGSSSDQQPSR VSHEQFRAALQLVVSPGDPREYLANFIKIGEGS TGIVCIATEKHTGKQVAVKKMDLRKQQRRELLF NEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFL EGGALTDIVTHTRMNEEQIATVCLSVLRALSYL HNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQ VSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDI WSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSL PPRVKDLHKVSSVLRGFLDLMLVREPSQRATAQ ELLGHPFLKLAGPPSCIVPLMRQYRHH*AEDSCR WQS*MRT*E*FRRTKGNTEHAKGLCILDQPIGG TA**PAGFNRPGHLLVS*TGISPLTAGVVTWST ALISHYYIFQPFIQQIRRTQYKLRYDIS*PHAG* HVGFSILKEYFSGKKKKKRKKGKQKALFS*W*Q YNVFCNEFVIFLYDSFDNL*YFDVM*PLYQLK* YLFTRGV*TKPFLLFYPFKRTNDSLGTLNTE*LS ITVSFSKISFLS*QVSYLVEEELRVMVMVCTFH *SNQK**NKI*ATVYHSLG*S*IFLKITFSMNA SSSKPFFCTPQCFPQCPFSNGYNVPLWPNFPPRE QFQC*DHWIQFPK*NVSVRP*EFQAHKGRGENR ARRLVSFVTIFKTLYASTPNSCLYLPLYHSINR YCSCIVLEV*TDSRG*ISKRVFYQV*SDFSCAHV IMCFRHRRVVLLSRSS*MLIKMNDYYNTFCVAC WMNLHVNCRPI*ICL*NSGRAT*SSLVSINYAS DKSHLLPNWENRGFS*LFHIL*QI*I*ICDSALV ALSIIKNFCN**VANYLSLLKRKKGIK*PSTD* IFSFLSNWKCLHKYDL*FCFIKQQINVLCKILK H*YKYVRIKTI*IQKMAVLNVHETDCINLTRTM* K*KEKKKKIFFKPE*TLKTIAENSGFWIPNIFD SVMEIFL*FSYHRVFFKVFIEFTKSYCSLKGFV STNYWQKLHLQIKINVCL

Frame 1

SSSSPACARGDREHGAGSAVAAEKGLRHRRFAD SRPPGFCLPV*SYPAPWLLLGPLKCLSPNLCLQ FQPLP*ASKLYFQLSLDASIWKYTVVKCFHLLLK *TLRKNEED*QAPAKSCRIEIATLLWSL*FIHS HHIKVLASCLGRKRKRLKYLARPTLNTGFILGL IHKSRSLPAFPSSGTAC*QIRPTGQSLWWTLHAS HPSSWLL*RQSLEETNPARKPPSTAC*RILTTS R*LAPTP*GKKAHPPQIREPPATVQATRKKMAS SPSPSIPANPILLLTTRPKSTGRRVSMEMIWIRI IEAATQPSKMGT**K*STGRPTILR*SL*NPIL PDFLPIITHIWTH*ANQVNTVTSSGSIREPRVA PLWIIHSNSHLLELQGPAGAPRRAWRTVKVNGDP AWMTMTGGQSLRT*IRQALSPPCGRGPGQARDS RNR*CHLEQVHLKPIPKDTPTTPTPTLACPSPQ CAFQRWITIEHRWSSALHCQGLTPTPGALPNYLK VKANRAIPQAVTSTRLGTTKPPCTITPPCRAVR STSPRLPT*APSASHPAPTRRPAGAPPPTSSPP GCPMNSFGRPCSWWSAQETPGNTWPTLSKSGKAQ PASYASPPRNTQGNKLQ*RKWTSGSNRDENCFS MRS*SCGITTMTMWLTCTAATLSAMSSGWSWSF *KVVP*QTL*LTPE*MKNR*LLSACQF*ELSPTF ITKE*FTGT*KVTPSS*QAMAG*SCLILVSVLK FPKRCRRGNHWLALPTGWPLR*FLGYLMGQRWT SGPSGSW**K*LMASPPTSMSLPSRRCGGSGTVY LQE*RTYTRFLQCSGDS*T*CW*GSPLREQQPR NSSDIHS*N*QVHRLASSPS*DNTGITEQRIRV GGKAR*GHENNSGEQKETQNMQKACAF*TSQLVG QRDDRQGSTDQGIFLCLKQASLH*QPVWSLGAR L**VIIIFFSPSSSKSEGLSTNSVMIYPSHMQG NM*DFLY*KNTFLAKKKKKERKENKKHFFLNGSS IMYFATNL*FFCTIVLIIYSTLMSCSHCIS*SN TCLLEEFEQSLSYFFIPLREPMIL*EL*ILNDS QSPSALVKSLSYPNKCLIWWKKN*E*W*WCARFI NPTKNNEIKFEPQYTTPWDKVKYF*RSHFP*TP LVANHSFAHHNVSLSALSQMGTMFPCGQISLPG SNFSARIIGFSSQNRMFQ*DHENSRLTKGEERTG QDVWFHLSPFLKLCMLAHQTLVYIYLCTTVLIA IVHVSCWKSELTLEDELARGYFTRYDLTSVVPM L*CVSDIGESCCCLDLLEC**K*MTTTIHFVLLV G*ICMLTVGQYRFAFKTLEELHSHH*FLLIMHQ TKAICYQTGKTEAFLNYFTYCNKYEFKFVIALW LL*A*LRIFVINRLLIIYHC*KGKKA*NDLLLIR FSVFFQTGNASINMIYDFAS*NSKSMFYVKY*S ININM*E*KQSKSRKWQS*MFMRQIVLI*PGLC RSRKKRKRKSFLNQNKH*KLLQKIVDFGFQTFST V*WKFFCNFLTIGYFLKYSLSLPKVTVA*KVL* ALTIGRNCICK*K*MFAF

Frame 2

VVVPQRAPGETGNMALGAL*QLRRG*GTAASLT AGHQVSVSQYNHIQPHGSYWGL*NVYHQICVCS SNLFPEHQSCISNFRWMLLSGNTLW*NASTSC* NEH*GKMKKTDKHQRKVAE*K*PHSSGVFNSSTA II*RFWHHVWEEKEKD*NIWPVQL*TQGSYWV* STRAEVYRPSPAVAQPVSRYGQQAKAYGGPFMH HTHPAGSYEDNR*RKQTLQGNLHQRPARGF*QHL GDSLQLPKERKPTHPRSGSLQPRSRPRGRKWLH HLLPVFQRIRYYC*LHDRKVQGEESLWR*SGSV L*RQPRSQAKWARNENEARGGLLF*GEAFEIRFC QIFCRLSLTFGLTEQTK*IQ*PQVGVSESLE*L PSGLFIPIHTF*NCRDQRVLQGEPGVQ*K*MGT QPG*L*QEAKVFVPESDKPSAHHAAEVQVRLGTP GTDDAIWSKCI*NPSPRTLLQLLHLPSLVRAHN VHSKGGLRSSTDGPQPSTVRV*HLPQGPCQTTS KSKQIGLFLKQSPVPVWVPQSHLVPSPLPAEQFA VHLHGFLPELPQPLIQHLPAAQLGLLLRPAALQ GVP*TVSGGPAAGGQPRRPQGILGQLYQNRGRL NRHRMHRHRETHRETSCSEENGPPEATETRTAFQ *GRDHAGLPP*QCG*HVQQLPCRR*ALGGHGVS RRWCLDRHCDSHQNE*RTDSYCLPVSSESSLLP S*PRSDSQGHKK*LHPPDKRWPDKVV*FWFLCSS FQRGAEEEIIGWHSLLDGP*GDF*ATLWDRGGH LVPRDHGDRND*WRAPLLQ*ASPPGDAEDPGQF TSKSEGPTQGFFSAPGIPRLDVGEGALSESNSPG TPRTSILKTSRSTVLHRPPHETIQASLSRGFV* VAKLDEDMRIIQENKRKHRTCKRPVHSRPANWW DSVMTGRVQQTRASSCVLNRHLSTDSRCGHLEHG FNKSLLYFSALHPANQKDSVQTPL*YILATCRV TCRIFYIERILFWQKKKKKKERKTKSTFFLMVA V*CILQRICNFSVR*F**FIVL*CHVAIVSVEVI LVY*RSLNKAFPTFLSL*ENQ*FFRNFEY*MTL NHRQL**NLFLILTSVLFGGRRIKSDGDGVHVS LIQPKIMK*NLSHSIPLLGIKLNIFKDHIFHERL **QTILLHTTMFPSVPFLKWVQCSLVAKFPSQG AISVLGSLDSVPKIECFSETMRIPGSQRERREQ GKTFGFICHHF*NSVC*HTKLLSIFTFVPQY*SL LFMYRAGSLN*L*RMN*QEGILPGMI*LQLCPC YNVFPT*ESRAAV*IFLNVDKNE*LLQYILCCL LDEFAC*L*ANIDLPLKLWKSYIVIISFY*LCIR QKPFVTKLGKQRLFLTISHTVTNMNLNL**RSG CSKHN*EFL*LIGC*LFITAKKEKRHKMTFY*L DFQFSFKLEMPP*I*SMILLHKTANQCFM*NIKA LI*ICENKNNLNPENGSPKCS*DRLY*FNQDYV EVERKEKENLF*TRINIKNYCRK*WILDSKHFR QCNGNFSVIFLPSGIF*SIH*VYQKLL*LKRFCE H*LLAETAFANKNKCLPF