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Sequences

Sequence NM_001014833

AGCCCCGGATGTTCGTTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAGTTCCAG GTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACC AGCATCAGACCAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCA CACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGAC CCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGT TTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAGCCCCC TGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTAC CTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACC TGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGT GGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTG TGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGG TGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCAT CTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCC CTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGG TGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCA GAACCGCACCAGATGAGGCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAGTAGGGGGCCAGGCCTCCCACT CCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGA AAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCTCCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCGGC CCTCCCATCACTGGAAGTCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTGCACGCGTGTGAGTGTGCATGT GTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCTGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATTGTGGG GGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTGGATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCA GCCCCTGCAGCAAATGACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACACTCTAGCCCCTGCCCTTATTGG GGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCCCT GCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTTTTGTGAAA

refseq_PAK4.F1 (forward primer)
refseq_PAK4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPGCSLGIQHGGGSVRGGGGARELREARGRGVP GRAVRPRATAAPSR*VTRSP*RSGHLPEGTSVPP ASDQAAPSPRHHVWEEEEAGGDLRAVQLRAPRA HGLRPARAEVHGAAPPVAEPDRGVGSPAQAPRR PRLHHLHPARGPQDHRAGQQRCQRWGPHAAAGRV *EHVGDTLQLPAERQPAAARPCPPGKWDARGAP WPPLTTAGATASIP*AVPGCPAAGGGPRRPPLL PGQLHQDWRGLHGHRVHRHRAQLGQAGGRQEDGP AQAAEARAALQRGGNHEGLPARECGGDVQQLPG GGRALGGHGVPGRRRPHRHRHPHQDERGADRGR VPCSAAGPVGAPRPGRHPPGHQERLDPADP*WQG EAVRLWVLRPGEQGSAPKEVAGRHALLDGPRAH LPPSLRARGRHLVAGDNGD*DGGRRAPLLQRAT PQSHEDDSGQPATPTEEPAQGVAIPEGLPGPPAG ARPCPAGHGSRAAEAPIPGQGRAACQHRAPHAP EPHQMRPSALPLNQRAPRVTPAPLRPVGGQASH SSQPGRCSAWHHPPCWG*MRPYY*TPVLIS*LLE KHRDSWEQARLPGPPPSGTGPPPCSSVSRKGSG PPITGSLQWGSLGVERTLRGEHV*VCARV*VCM CVCAKVQPPRPPACKGCLAPCLTPSPLSP*AIVG VDHECPKSGLFP*PCAPFLWLDGETGQGPPPSP APAANDYCTWTASSFLEVYLYCHFITL*PLPLL GDRWSLSCGVALAEPLPEEPGSCPVSAAPARPPL PRVSFTIKTLSCFVK

Frame 1

APDVRWGFNMAAGVSAVVAVQES*GRREGAEFQ VEQLGRERLRRRADE*PEAPRGVVTCLRALLSH QHQTRPHRVPGTMFGKRKKRVEISAPSNFEHRVH TGFDQHEQKFTGLPRQWQSLIEESARRPKPLVD PACITSIQPGAPKTIVRGSKGAKDGALTLLLDE FENMSVTRSNSLRRDSPPPPARARQENGMPEEPP GPRSPQREPQRVSHEQFRAALQLVVDPGDPRSY LDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMD LRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLV GDELWVVMEFLEGGALTDIVTHTRMNEEQIAAV CLAVLQALSVLHAQGVIHRDIKSDSILLTHDGR VKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELI SRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPP LKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLL VRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQ NRTR*GPAPFPSTKEPPGSPPPH*GQ*GARPPT PPSPGDAPRGTTLLAGGR*DPTTELQF*SRDF* KNTGTRGSKRGSQDPHPLGQALPHVLLSPGRAAA LPSLEVCSGGRWGWREH*EVNMYECVHACECAC VCVQRSSHPVLQPARGVWRLA*HPAPSPPEPLW GSIMNVRRVAFSRSPAPPFCGWMGRQVRAPHPLQ PLQQMTTAPGQPPLF*KSIYIVIL*HSSPCPYW GTDGPCPAGWPWQNHCLKNQVPARSAQPQPAHP CLELVLQLKHCLVL*

Frame 2

PRMFVGDSTWRRECPRWWRCKRAEGGARARSSR SSS*AASDCGAEPMSNPKPLEEWSPA*GHFCPT SIRPGRTESPAPCLGRGRSGWRSPRRPTSSTAC TRASTSTSRSSRGCPASGRA*SRSRLAGPSPSST PPASPPSSPGPPRPSCGAAKVPKMGPSRCCWTS LRTCR*HAPTPCGETARRRPPVPARKMGCQRSP LAPAHHSGSHSEYPMSSSGLPCSWWWTQATPAPT WTTSSRLARAPRASCASPPCAARASWWPSRRWT CASSRGASCSSTRW*S*GTTSTRMWWRCTTATW WGTSSGWSWSSWKEAPSPTSSPTPG*TRSRSRPC ALQCCRPCRCSTPRASSTGTSRATRSC*PMMAG *SCQTLGSAPR*ARKCPEGSRWSARPTGWPQSS SPAFPTGQR*TSGRWG*W*LRWWTESPPTSTSHP SKP*R*FGTTCHPD*RTCTRCRHP*RASWTACW CETLPSGPRQPSC*STHSWPRQGRLPASCPSCA RTAPDEAQRPSPQPKSPPGHPRPTEASRGPGLPL LPAREMLRVAPPSLLGVDETLLLNSSFDLVTFR KTQGLVGASEAPRTPTLWDRPSPMFFCLQEGQR PSHHWKSAVGVAGGGENTKR*TCMSVCTRVSVHV CVCKGPATPSSSLQGVSGALPDTQPPLPLSHCG GRS*MSEEWPFPVALRPLSVAGWGDRSGPPTLS SPCSK*LLHLDSLLFSRSLFILSFYNTLAPALIG GQMVPVLRGGPGRTTA*RTRFLPGQRSPSPPTP ASS*FYN*NIVLFCE

Sequence NM_001014835

AGCCCCGGATGTTCGTTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAGTTCCAG GTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACC AGCATCAGACCAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCA CACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGAC CCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCCCAGT CCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGC CTGCACCCCCGCCGCCCCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTG CAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTGCGCA GCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCA CGAGAATGTGGTGGAGATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACC CACACCAGGATGAACGAGGAGCAGATCGCGGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGACATCA AGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCT GGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATG GTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAGGTGT CGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGC AGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAGGCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCG CCCCACTGAGGCCAGTAGGGGGCCAGGCCTCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAGATGAGACCCTA CTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGAAAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCT CCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCGGCCCTCCCATCACTGGAAGTCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAAC ATGTATGAGTGTGTGCACGCGTGTGAGTGTGCATGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCTGCAAGGGGTGTCTGGCGCCTTGCCT GACACCCAGCCCCCTCTCCCCCTGAGCCATTGTGGGGGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTG GATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAATGACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATT GTCATTTTATAACACTCTAGCCCCTGCCCTTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAAGAACCAGGTTC CTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCCCTGCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTTTTGTG

refseq_PAK4.F1 (forward primer)
refseq_PAK4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPGCSLGIQHGGGSVRGGGGARELREARGRGVP GRAVRPRATAAPSR*VTRSP*RSGHLPEGTSVPP ASDQAAPSPRHHVWEEEEAGGDLRAVQLRAPRA HGLRPARAEVHGAAPPVAEPDRGVGSPAQAPRR PRLHHLHPARGPQGGAS*RGP*RAISGGPGHPPV LLLLLPASHPSPRCPQPWSAGTPRLRAPAGPSS LHPRRPCCSWAPWPPLTTAGATASIP*AVPGCP AAGGGPRRPPLLPGQLHQDWRGLHGHRVHRHRAQ LGQAGGRQEDGPAQAAEARAALQRGGNHEGLPA RECGGDVQQLPGGGRALGGHGVPGRRRPHRHRH PHQDERGADRGRVPCSAAGPVGAPRPGRHPPGHQ ERLDPADP*WQGEAVRLWVLRPGEQGSAPKEVA GRHALLDGPRAHLPPSLRARGRHLVAGDNGD*D GGRRAPLLQRATPQSHEDDSGQPATPTEEPAQGV AIPEGLPGPPAGARPCPAGHGSRAAEAPIPGQG RAACQHRAPHAPEPHQMRPSALPLNQRAPRVTP APLRPVGGQASHSSQPGRCSAWHHPPCWG*MRPY Y*TPVLIS*LLEKHRDSWEQARLPGPPPSGTGP PPCSSVSRKGSGPPITGSLQWGSLGVERTLRGE HV*VCARV*VCMCVCAKVQPPRPPACKGCLAPCL TPSPLSP*AIVGVDHECPKSGLFP*PCAPFLWL DGETGQGPPPSPAPAANDYCTWTASSFLEVYLY CHFITL*PLPLLGDRWSLSCGVALAEPLPEEPGS CPVSAAPARPPLPRVSFTIKTLSCFV

Frame 1

APDVRWGFNMAAGVSAVVAVQES*GRREGAEFQ VEQLGRERLRRRADE*PEAPRGVVTCLRALLSH QHQTRPHRVPGTMFGKRKKRVEISAPSNFEHRVH TGFDQHEQKFTGLPRQWQSLIEESARRPKPLVD PACITSIQPGAPKGEPHDVAPNGPSAGGLAIPQ SSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPA CTPAAPAVPGPPGPRSPQREPQRVSHEQFRAAL QLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVR SSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQH ENVVEMYNSYLVGDELWVVMEFLEGGALTDIVT HTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDI KSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSL VGTPYWMAPELISRLPYGPEVDIWSLGIMVIEM VDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKV SPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKA GPPASIVPLMRQNRTR*GPAPFPSTKEPPGSPP PH*GQ*GARPPTPPSPGDAPRGTTLLAGGR*DP TTELQF*SRDF*KNTGTRGSKRGSQDPHPLGQAL PHVLLSPGRAAALPSLEVCSGGRWGWREH*EVN MYECVHACECACVCVQRSSHPVLQPARGVWRLA *HPAPSPPEPLWGSIMNVRRVAFSRSPAPPFCGW MGRQVRAPHPLQPLQQMTTAPGQPPLF*KSIYI VIL*HSSPCPYWGTDGPCPAGWPWQNHCLKNQV PARSAQPQPAHPCLELVLQLKHCLVL

Frame 2

PRMFVGDSTWRRECPRWWRCKRAEGGARARSSR SSS*AASDCGAEPMSNPKPLEEWSPA*GHFCPT SIRPGRTESPAPCLGRGRSGWRSPRRPTSSTAC TRASTSTSRSSRGCPASGRA*SRSRLAGPSPSST PPASPPSSPGPPRGSLMTWPLTGHQRGAWPSPS PPPPPPGLPPEPEVPPALECWDPTPQSPSWPLQ PAPPPPLLFLGPLAPAHHSGSHSEYPMSSSGLPC SWWWTQATPAPTWTTSSRLARAPRASCASPPCA ARASWWPSRRWTCASSRGASCSSTRW*S*GTTS TRMWWRCTTATWWGTSSGWSWSSWKEAPSPTSSP TPG*TRSRSRPCALQCCRPCRCSTPRASSTGTS RATRSC*PMMAG*SCQTLGSAPR*ARKCPEGSR WSARPTGWPQSSSPAFPTGQR*TSGRWG*W*LRW WTESPPTSTSHPSKP*R*FGTTCHPD*RTCTRC RHP*RASWTACWCETLPSGPRQPSC*STHSWPR QGRLPASCPSCARTAPDEAQRPSPQPKSPPGHPR PTEASRGPGLPLLPAREMLRVAPPSLLGVDETL LLNSSFDLVTFRKTQGLVGASEAPRTPTLWDRP SPMFFCLQEGQRPSHHWKSAVGVAGGGENTKR*T CMSVCTRVSVHVCVCKGPATPSSSLQGVSGALP DTQPPLPLSHCGGRS*MSEEWPFPVALRPLSVA GWGDRSGPPTLSSPCSK*LLHLDSLLFSRSLFIL SFYNTLAPALIGGQMVPVLRGGPGRTTA*RTRF LPGQRSPSPPTPASS*FYN*NIVLFC

Sequence NM_005884

AGCCCCGGATGTTCGTTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAGTTCCAG GTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACC AGCATCAGACCAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCGCCGTCCAACTTCGAGCACCGCGTGCA CACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGGCTCGCCGGCCCAAGCCCCTCGTCGAC CCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGGGGCCCTCACGCTGCTGCTGGACGAGT TTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAGGAAAATGGGATGCCAGAGGAGCCGGC CACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCGAGGCGGGTGGCGGCAGTGGTGACAGGCGACGGGCGGGG CCAGAGAAGAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACGCCCCCTCTCCGGGCCTGATGTCGGCACCCCCC AGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCAGCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACGGACCACCCATCCCGGGGTGCCCAGGG GGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCCCAGTCCTCCTCCTCCTCCTCCCGGCCTCCCACCCGAGCCCGAGGT GCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCCCGCCGCCCCTGCTGTTCCTGGGCCCCCTGGCC CCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGACCCAGGCGACCCCCGCTCCTACCTGGA CAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGGTGGCCGTCAAGAAGATGGACCTGCGC AAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGAGATGTACAACAGCTACCTGGTGGGGG ACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAACGAGGAGCAGATCGCGGCCGTGTGCCT TGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCCTGCTGACCCATGATGGCAGGGTGAAG CTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTACTGGATGGCCCCAGAGCTCATCTCCC GCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCCCCCTACTTCAACGAGCCACCCCTCAA AGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGGGCTTCCTGGACCGCCTGCTGGTGCGA GACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAGCATCGTGCCCCTCATGCGCCAGAACC GCACCAGATGAGGCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAGTAGGGGGCCAGGCCTCCCACTCCTCC CAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGTTTTGATCTCGTGACTTTTAGAAAAAC ACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCTCCCCCATGTTCTTCTGTCTCCAGGAAGGGCAGCGGCCCTCC CATCACTGGAAGTCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTGCACGCGTGTGAGTGTGCATGTGTGTG TGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCTGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCTCTCCCCCTGAGCCATTGTGGGGGTCG ATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTGGATGGGGAGACAGGTCAGGGCCCCCCACCCTCTCCAGCCCC TGCAGCAAATGACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACACTCTAGCCCCTGCCCTTATTGGGGGAC AGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGCAGCCCCAGCCCGCCCACCCCTGCCTC GAGTTAGTTTTACAATTAAAACATTGTCTTGTTTTGTG

refseq_PAK4.F1 (forward primer)
refseq_PAK4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPGCSLGIQHGGGSVRGGGGARELREARGRGVP GRAVRPRATAAPSR*VTRSP*RSGHLPEGTSVPP ASDQAAPSPRHHVWEEEEAGGDLRAVQLRAPRA HGLRPARAEVHGAAPPVAEPDRGVGSPAQAPRR PRLHHLHPARGPQDHRAGQQRCQRWGPHAAAGRV *EHVGDTLQLPAERQPAAARPCPPGKWDARGAG HHGQRGPREGRQPRPVRRSQRGGWRQW*QATGG AREEAQVFQGGLRGSPGVLPGQTPPLRA*CRHPP ACWSGQWGETGSWPAL*HLPEG*HGPPIPGCPG GAS*RGP*RAISGGPGHPPVLLLLLPASHPSPR CPQPWSAGTPRLRAPAGPSSLHPRRPCCSWAPWP PLTTAGATASIP*AVPGCPAAGGGPRRPPLLPG QLHQDWRGLHGHRVHRHRAQLGQAGGRQEDGPA QAAEARAALQRGGNHEGLPARECGGDVQQLPGGG RALGGHGVPGRRRPHRHRHPHQDERGADRGRVP CSAAGPVGAPRPGRHPPGHQERLDPADP*WQGE AVRLWVLRPGEQGSAPKEVAGRHALLDGPRAHLP PSLRARGRHLVAGDNGD*DGGRRAPLLQRATPQ SHEDDSGQPATPTEEPAQGVAIPEGLPGPPAGA RPCPAGHGSRAAEAPIPGQGRAACQHRAPHAPEP HQMRPSALPLNQRAPRVTPAPLRPVGGQASHSS QPGRCSAWHHPPCWG*MRPYY*TPVLIS*LLEK HRDSWEQARLPGPPPSGTGPPPCSSVSRKGSGPP ITGSLQWGSLGVERTLRGEHV*VCARV*VCMCV CAKVQPPRPPACKGCLAPCLTPSPLSP*AIVGV DHECPKSGLFP*PCAPFLWLDGETGQGPPPSPAP AANDYCTWTASSFLEVYLYCHFITL*PLPLLGD RWSLSCGVALAEPLPEEPGSCPVSAAPARPPLP RVSFTIKTLSCFV

Frame 1

APDVRWGFNMAAGVSAVVAVQES*GRREGAEFQ VEQLGRERLRRRADE*PEAPRGVVTCLRALLSH QHQTRPHRVPGTMFGKRKKRVEISAPSNFEHRVH TGFDQHEQKFTGLPRQWQSLIEESARRPKPLVD PACITSIQPGAPKTIVRGSKGAKDGALTLLLDE FENMSVTRSNSLRRDSPPPPARARQENGMPEEPA TTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAG PEKRPKSSREGSGGPQESSRDKRPLSGPDVGTP QPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQG EPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARG APSPGVLGPHASEPQLAPPACTPAAPAVPGPPG PRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLR KQQRRELLFNEVVIMRDYQHENVVEMYNSYLVG DELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCL AVLQALSVLHAQGVIHRDIKSDSILLTHDGRVK LSDFGFCAQVSKEVPRRKSLVGTPYWMAPELIS RLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLK AMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVR DPAQRATAAELLKHPFLAKAGPPASIVPLMRQN RTR*GPAPFPSTKEPPGSPPPH*GQ*GARPPTPP SPGDAPRGTTLLAGGR*DPTTELQF*SRDF*KN TGTRGSKRGSQDPHPLGQALPHVLLSPGRAAAL PSLEVCSGGRWGWREH*EVNMYECVHACECACVC VQRSSHPVLQPARGVWRLA*HPAPSPPEPLWGS IMNVRRVAFSRSPAPPFCGWMGRQVRAPHPLQP LQQMTTAPGQPPLF*KSIYIVIL*HSSPCPYWGT DGPCPAGWPWQNHCLKNQVPARSAQPQPAHPCL ELVLQLKHCLVL

Frame 2

PRMFVGDSTWRRECPRWWRCKRAEGGARARSSR SSS*AASDCGAEPMSNPKPLEEWSPA*GHFCPT SIRPGRTESPAPCLGRGRSGWRSPRRPTSSTAC TRASTSTSRSSRGCPASGRA*SRSRLAGPSPSST PPASPPSSPGPPRPSCGAAKVPKMGPSRCCWTS LRTCR*HAPTPCGETARRRPPVPARKMGCQRSR PPRPEGAQGRQAAEAGSPVTARRVAAVVTGDGRG QRRGPSLPGRAQGVPRSPPGTNAPSPGLMSAPP SLLVWPVGRNWQLAGPLTPTRGLTRTTHPGVPR GSLMTWPLTGHQRGAWPSPSPPPPPPGLPPEPEV PPALECWDPTPQSPSWPLQPAPPPPLLFLGPLA PAHHSGSHSEYPMSSSGLPCSWWWTQATPAPTW TTSSRLARAPRASCASPPCAARASWWPSRRWTCA SSRGASCSSTRW*S*GTTSTRMWWRCTTATWWG TSSGWSWSSWKEAPSPTSSPTPG*TRSRSRPCA LQCCRPCRCSTPRASSTGTSRATRSC*PMMAG*S CQTLGSAPR*ARKCPEGSRWSARPTGWPQSSSP AFPTGQR*TSGRWG*W*LRWWTESPPTSTSHPS KP*R*FGTTCHPD*RTCTRCRHP*RASWTACWCE TLPSGPRQPSC*STHSWPRQGRLPASCPSCART APDEAQRPSPQPKSPPGHPRPTEASRGPGLPLL PAREMLRVAPPSLLGVDETLLLNSSFDLVTFRKT QGLVGASEAPRTPTLWDRPSPMFFCLQEGQRPS HHWKSAVGVAGGGENTKR*TCMSVCTRVSVHVC VCKGPATPSSSLQGVSGALPDTQPPLPLSHCGGR S*MSEEWPFPVALRPLSVAGWGDRSGPPTLSSP CSK*LLHLDSLLFSRSLFILSFYNTLAPALIGG QMVPVLRGGPGRTTA*RTRFLPGQRSPSPPTPAS S*FYN*NIVLFC

Sequence NM_001014831

AGCCCCGGATGTTCGTTGGGGATTCAACATGGCGGCGGGAGTGTCCGCGGTGGTGGCGGTGCAAGAGAGCTGAGGGAGGCGCGAGGGCGCGGAGTTCCAG GTCGAGCAGTTAGGCCGCGAGCGACTGCGGCGCCGAGCCGATGAGTAACCCGAAGCCCCTAGAGGAGTGGTCACCTGCCTGAGGGCACTTCTGTCCCACC AGCATCAGACCAGGTGTGTGCAGGCTCTCTATGGGATGCCGCCATCAGAATGTCTCTTCCTCAGCCCCTTTGTTTTTTAAAGTGGCTTTGGTCGACCATT TGCTCTGTGCTTGGTACTGTGTCAAGGGCCCGATTGAAGACCACAGATCCTAAAGATAGGGGAGTGTCGCGACAAGGCTGCCTGGGGTGCCCTCTTAACC CAGGGGATCAGTAACCCTGTGCTGAGTCAATATCCAAAGGCCGCACCGAGTCCCCGGCACCATGTTTGGGAAGAGGAAGAAGCGGGTGGAGATCTCCGCG CCGTCCAACTTCGAGCACCGCGTGCACACGGGCTTCGACCAGCACGAGCAGAAGTTCACGGGGCTGCCCCGCCAGTGGCAGAGCCTGATCGAGGAGTCGG CTCGCCGGCCCAAGCCCCTCGTCGACCCCGCCTGCATCACCTCCATCCAGCCCGGGGCCCCCAAGACCATCGTGCGGGGCAGCAAAGGTGCCAAAGATGG GGCCCTCACGCTGCTGCTGGACGAGTTTGAGAACATGTCGGTGACACGCTCCAACTCCCTGCGGAGAGACAGCCCGCCGCCGCCCGCCCGTGCCCGCCAG GAAAATGGGATGCCAGAGGAGCCGGCCACCACGGCCAGAGGGGGCCCAGGGAAGGCAGGCAGCCGAGGCCGGTTCGCCGGTCACAGCGAGGCGGGTGGCG GCAGTGGTGACAGGCGACGGGCGGGGCCAGAGAAGAGGCCCAAGTCTTCCAGGGAGGGCTCAGGGGGTCCCCAGGAGTCCTCCCGGGACAAACGCCCCCT CTCCGGGCCTGATGTCGGCACCCCCCAGCCTGCTGGTCTGGCCAGTGGGGCGAAACTGGCAGCTGGCCGGCCCTTTAACACCTACCCGAGGGCTGACACG GACCACCCATCCCGGGGTGCCCAGGGGGAGCCTCATGACGTGGCCCCTAACGGGCCATCAGCGGGGGGCCTGGCCATCCCCCAGTCCTCCTCCTCCTCCT CCCGGCCTCCCACCCGAGCCCGAGGTGCCCCCAGCCCTGGAGTGCTGGGACCCCACGCCTCAGAGCCCCAGCTGGCCCCTCCAGCCTGCACCCCCGCCGC CCCTGCTGTTCCTGGGCCCCCTGGCCCCCGCTCACCACAGCGGGAGCCACAGCGAGTATCCCATGAGCAGTTCCGGGCTGCCCTGCAGCTGGTGGTGGAC CCAGGCGACCCCCGCTCCTACCTGGACAACTTCATCAAGATTGGCGAGGGCTCCACGGGCATCGTGTGCATCGCCACCGTGCGCAGCTCGGGCAAGCTGG TGGCCGTCAAGAAGATGGACCTGCGCAAGCAGCAGAGGCGCGAGCTGCTCTTCAACGAGGTGGTAATCATGAGGGACTACCAGCACGAGAATGTGGTGGA GATGTACAACAGCTACCTGGTGGGGGACGAGCTCTGGGTGGTCATGGAGTTCCTGGAAGGAGGCGCCCTCACCGACATCGTCACCCACACCAGGATGAAC GAGGAGCAGATCGCGGCCGTGTGCCTTGCAGTGCTGCAGGCCCTGTCGGTGCTCCACGCCCAGGGCGTCATCCACCGGGACATCAAGAGCGACTCGATCC TGCTGACCCATGATGGCAGGGTGAAGCTGTCAGACTTTGGGTTCTGCGCCCAGGTGAGCAAGGAAGTGCCCCGAAGGAAGTCGCTGGTCGGCACGCCCTA CTGGATGGCCCCAGAGCTCATCTCCCGCCTTCCCTACGGGCCAGAGGTAGACATCTGGTCGCTGGGGATAATGGTGATTGAGATGGTGGACGGAGAGCCC CCCTACTTCAACGAGCCACCCCTCAAAGCCATGAAGATGATTCGGGACAACCTGCCACCCCGACTGAAGAACCTGCACAAGGTGTCGCCATCCCTGAAGG GCTTCCTGGACCGCCTGCTGGTGCGAGACCCTGCCCAGCGGGCCACGGCAGCCGAGCTGCTGAAGCACCCATTCCTGGCCAAGGCAGGGCCGCCTGCCAG CATCGTGCCCCTCATGCGCCAGAACCGCACCAGATGAGGCCCAGCGCCCTTCCCCTCAACCAAAGAGCCCCCCGGGTCACCCCCGCCCCACTGAGGCCAG TAGGGGGCCAGGCCTCCCACTCCTCCCAGCCCGGGAGATGCTCCGCGTGGCACCACCCTCCTTGCTGGGGGTAGATGAGACCCTACTACTGAACTCCAGT TTTGATCTCGTGACTTTTAGAAAAACACAGGGACTCGTGGGAGCAAGCGAGGCTCCCAGGACCCCCACCCTCTGGGACAGGCCCTCCCCCATGTTCTTCT GTCTCCAGGAAGGGCAGCGGCCCTCCCATCACTGGAAGTCTGCAGTGGGGGTCGCTGGGGGTGGAGAGAACACTAAGAGGTGAACATGTATGAGTGTGTG CACGCGTGTGAGTGTGCATGTGTGTGTGTGCAAAGGTCCAGCCACCCCGTCCTCCAGCCTGCAAGGGGTGTCTGGCGCCTTGCCTGACACCCAGCCCCCT CTCCCCCTGAGCCATTGTGGGGGTCGATCATGAATGTCCGAAGAGTGGCCTTTTCCCGTAGCCCTGCGCCCCCTTTCTGTGGCTGGATGGGGAGACAGGT CAGGGCCCCCCACCCTCTCCAGCCCCTGCAGCAAATGACTACTGCACCTGGACAGCCTCCTCTTTTCTAGAAGTCTATTTATATTGTCATTTTATAACAC TCTAGCCCCTGCCCTTATTGGGGGACAGATGGTCCCTGTCCTGCGGGGTGGCCCTGGCAGAACCACTGCCTGAAGAACCAGGTTCCTGCCCGGTCAGCGC AGCCCCAGCCCGCCCACCCCTGCCTCGAGTTAGTTTTACAATTAAAACATTGTCTTGTTTTGTGAAA

refseq_PAK4.F1 (forward primer)
refseq_PAK4.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

SPGCSLGIQHGGGSVRGGGGARELREARGRGVP GRAVRPRATAAPSR*VTRSP*RSGHLPEGTSVPP ASDQVCAGSLWDAAIRMSLPQPLCFLKWLWSTI CSVLGTVSRARLKTTDPKDRGVSRQGCLGCPLN PGDQ*PCAESISKGRTESPAPCLGRGRSGWRSPR RPTSSTACTRASTSTSRSSRGCPASGRA*SRSR LAGPSPSSTPPASPPSSPGPPRPSCGAAKVPKM GPSRCCWTSLRTCR*HAPTPCGETARRRPPVPAR KMGCQRSRPPRPEGAQGRQAAEAGSPVTARRVA AVVTGDGRGQRRGPSLPGRAQGVPRSPPGTNAP SPGLMSAPPSLLVWPVGRNWQLAGPLTPTRGLTR TTHPGVPRGSLMTWPLTGHQRGAWPSPSPPPPP PGLPPEPEVPPALECWDPTPQSPSWPLQPAPPP PLLFLGPLAPAHHSGSHSEYPMSSSGLPCSWWWT QATPAPTWTTSSRLARAPRASCASPPCAARASW WPSRRWTCASSRGASCSSTRW*S*GTTSTRMWW RCTTATWWGTSSGWSWSSWKEAPSPTSSPTPG*T RSRSRPCALQCCRPCRCSTPRASSTGTSRATRS C*PMMAG*SCQTLGSAPR*ARKCPEGSRWSARP TGWPQSSSPAFPTGQR*TSGRWG*W*LRWWTESP PTSTSHPSKP*R*FGTTCHPD*RTCTRCRHP*R ASWTACWCETLPSGPRQPSC*STHSWPRQGRLP ASCPSCARTAPDEAQRPSPQPKSPPGHPRPTEAS RGPGLPLLPAREMLRVAPPSLLGVDETLLLNSS FDLVTFRKTQGLVGASEAPRTPTLWDRPSPMFF CLQEGQRPSHHWKSAVGVAGGGENTKR*TCMSVC TRVSVHVCVCKGPATPSSSLQGVSGALPDTQPP LPLSHCGGRS*MSEEWPFPVALRPLSVAGWGDR SGPPTLSSPCSK*LLHLDSLLFSRSLFILSFYNT LAPALIGGQMVPVLRGGPGRTTA*RTRFLPGQR SPSPPTPASS*FYN*NIVLFCE

Frame 1

APDVRWGFNMAAGVSAVVAVQES*GRREGAEFQ VEQLGRERLRRRADE*PEAPRGVVTCLRALLSH QHQTRCVQALYGMPPSECLFLSPFVF*SGFGRPF ALCLVLCQGPD*RPQILKIGECRDKAAWGALLT QGISNPVLSQYPKAAPSPRHHVWEEEEAGGDLR AVQLRAPRAHGLRPARAEVHGAAPPVAEPDRGVG SPAQAPRRPRLHHLHPARGPQDHRAGQQRCQRW GPHAAAGRV*EHVGDTLQLPAERQPAAARPCPP GKWDARGAGHHGQRGPREGRQPRPVRRSQRGGWR QW*QATGGAREEAQVFQGGLRGSPGVLPGQTPP LRA*CRHPPACWSGQWGETGSWPAL*HLPEG*H GPPIPGCPGGAS*RGP*RAISGGPGHPPVLLLLL PASHPSPRCPQPWSAGTPRLRAPAGPSSLHPRR PCCSWAPWPPLTTAGATASIP*AVPGCPAAGGG PRRPPLLPGQLHQDWRGLHGHRVHRHRAQLGQAG GRQEDGPAQAAEARAALQRGGNHEGLPARECGG DVQQLPGGGRALGGHGVPGRRRPHRHRHPHQDE RGADRGRVPCSAAGPVGAPRPGRHPPGHQERLDP ADP*WQGEAVRLWVLRPGEQGSAPKEVAGRHAL LDGPRAHLPPSLRARGRHLVAGDNGD*DGGRRA PLLQRATPQSHEDDSGQPATPTEEPAQGVAIPEG LPGPPAGARPCPAGHGSRAAEAPIPGQGRAACQ HRAPHAPEPHQMRPSALPLNQRAPRVTPAPLRP VGGQASHSSQPGRCSAWHHPPCWG*MRPYY*TPV LIS*LLEKHRDSWEQARLPGPPPSGTGPPPCSS VSRKGSGPPITGSLQWGSLGVERTLRGEHV*VC ARV*VCMCVCAKVQPPRPPACKGCLAPCLTPSPL SP*AIVGVDHECPKSGLFP*PCAPFLWLDGETG QGPPPSPAPAANDYCTWTASSFLEVYLYCHFIT L*PLPLLGDRWSLSCGVALAEPLPEEPGSCPVSA APARPPLPRVSFTIKTLSCFVK

Frame 2

PRMFVGDSTWRRECPRWWRCKRAEGGARARSSR SSS*AASDCGAEPMSNPKPLEEWSPA*GHFCPT SIRPGVCRLSMGCRHQNVSSSAPLFFKVALVDH LLCAWYCVKGPIEDHRS*R*GSVATRLPGVPS*P RGSVTLC*VNIQRPHRVPGTMFGKRKKRVEISA PSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEES ARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDG ALTLLLDEFENMSVTRSNSLRRDSPPPPARARQ ENGMPEEPATTARGGPGKAGSRGRFAGHSEAGG GSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPL SGPDVGTPQPAGLASGAKLAAGRPFNTYPRADT DHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSS SRPPTRARGAPSPGVLGPHASEPQLAPPACTPAA PAVPGPPGPRSPQREPQRVSHEQFRAALQLVVD PGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKL VAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVE MYNSYLVGDELWVVMEFLEGGALTDIVTHTRMN EEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSI LLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPY WMAPELISRLPYGPEVDIWSLGIMVIEMVDGEP PYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLK GFLDRLLVRDPAQRATAAELLKHPFLAKAGPPAS IVPLMRQNRTR*GPAPFPSTKEPPGSPPPH*GQ *GARPPTPPSPGDAPRGTTLLAGGR*DPTTELQ F*SRDF*KNTGTRGSKRGSQDPHPLGQALPHVLL SPGRAAALPSLEVCSGGRWGWREH*EVNMYECV HACECACVCVQRSSHPVLQPARGVWRLA*HPAP SPPEPLWGSIMNVRRVAFSRSPAPPFCGWMGRQV RAPHPLQPLQQMTTAPGQPPLF*KSIYIVIL*H SSPCPYWGTDGPCPAGWPWQNHCLKNQVPARSA QPQPAHPCLELVLQLKHCLVL*