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Sequences

Sequence NM_004154

GTCAGCATTGGTGTAAAGGACTGGAGCTTCAGGGTTCTCGGGATTCGAGTCCTGGCCCTGCTGCTTGCCAGCTCTGTGCTGTGGCAAGTTACTTCCTCTC
CCCAGGTCTCTCGGTTTCCTCATCTGCTGCCTCTCCAGACTTCTGCCAGAACATTGCACGCGACAGTTTCAGGCACAGAACTGACTGGCAGCAGGGGCTG
CTCCACGAGTGGGAATTTGCTCCAGCACTTCACGGACTGCAAGCGAGGCACTTGCTAACTCTTGGCCTCCCTGAACATAGGAAACCCACCTGGGCAGCCA
TGGAATGGGACAATGGCACAGGCCAGGCTCTGGGCTTGCCACCCACCACCTGTGTCTACCGCGAGAACTTCAAGCAACTGCTGCTGCCACCTGTGTATTC
GGCGGTGCTGGCGGCTGGCCTGCCGCTGAACATCTGTGTCATTACCCAGATCTGCACGTCCCGCCGGGCCCTGACCCGCACGGCCGTGTACACCCTAAAC
CTTGCTCTGGCTGACCTGCTATATGCCTGCTCCCTGCCCCTGCTCATCTACAACTATGCCCAAGGTGATCACTGGCCCTTTGGCGACTTCGCCTGCCGCC
TGGTCCGCTTCCTCTTCTATGCCAACCTGCACGGCAGCATCCTCTTCCTCACCTGCATCAGCTTCCAGCGCTACCTGGGCATCTGCCACCCGCTGGCCCC
CTGGCACAAACGTGGGGGCCGCCGGGCTGCCTGGCTAGTGTGTGTAGCCGTGTGGCTGGCCGTGACAACCCAGTGCCTGCCCACAGCCATCTTCGCTGCC
ACAGGCATCCAGCGTAACCGCACTGTCTGCTATGACCTCAGCCCGCCTGCCCTGGCCACCCACTATATGCCCTATGGCATGGCTCTCACTGTCATCGGCT
TCCTGCTGCCCTTTGCTGCCCTGCTGGCCTGCTACTGTCTCCTGGCCTGCCGCCTGTGCCGCCAGGATGGCCCGGCAGAGCCTGTGGCCCAGGAGCGGCG
TGGCAAGGCGGCCCGCATGGCCGTGGTGGTGGCTGCTGCCTTTGCCATCAGCTTCCTGCCTTTTCACATCACCAAGACAGCCTACCTGGCAGTGCGCTCG
ACGCCGGGCGTCCCCTGCACTGTATTGGAGGCCTTTGCAGCGGCCTACAAAGGCACGCGGCCGTTTGCCAGTGCCAACAGCGTGCTGGACCCCATCCTCT
TCTACTTCACCCAGAAGAAGTTCCGCCGGCGACCACATGAGCTCCTACAGAAACTCACAGCCAAATGGCAGAGGCAGGGTCGCTGAGTCCTCCAGGTCCT
GGGCAGCCTTCATATTTGCCATTGTGTCCGGGGCACCAGGAGCCCCACCAACCCCAAACCATGCGGAGAATTAGAGTTCAGCTCAGCTGGGCATGGAGTT
AAGATCCCTCACAGGACCCAGAAGCTCACCAAAAACTATTTCTTCAGCCCCTTCTCTGGCCCAGACCCTGTGGGCATGGAGATGGACAGACCTGGGCCTG
GCTCTTGAGAGGTCCCAGTCAGCCATGGAGAGCTGGGGAAACCACATTAAGGTGCTCACAAAAATACAGTGTGACGTGTACTGTCATCAAGGGGTATGCT
CCATGCTTTGAGTCACCAATGAAGCGGGTGAGGGAAGATGAGAGGGGGAGGTGAGAGCTTCTGGGAAGGGGCATTTGAGCTGGGTTTTGAGGGATGATTA
TGAGCTCTCTGGAGAGGAGTGATATTCCATTTATTTAGAAAGCCTTTACTGACACCTTGTGCTCAGGCCTGTGTTGGTTCTGGGGCCCTAGAAGAACCAG
TCCTAGCCCTGGTCCATACGGGCTCCCAACTGCTGGAAGTACAGACTGGCACAGCAACATCAGGGTTGTGACAGAGGGAAGCATGGCTGGGGGGAGGGGG
TACACAGACAGTGCCCATGACCCAGTCCAAGGAGTCAGGAAAGAGCTCTCTGAGGAGGGAGCATCTGAGCCAGATGTTGAGGGCTGAGTGGGAACTTGGC
AAGCAGAAGTGGGGAGCACTTTAATGCAACCCAGGTATGCTCCATGCATATCCAGCTGGGCCAGCCTCGTGCTGGGCTCTGCCCTGGGCAGACAGGCAGA
GGGCCAGAGCAGAGGACACATGGCCTTGCGTGTGTGAAAGCTGAGAAAATGGGAGCTGTGCTTCAGCTACCCTCCAGACAAGGGCAAGAGTTAGCCAGAT
GCTCCAGGCAGTGGGAAGCCAATGGAGGGATTAAGCGGCGGAAGCTTCTTAGAAGAGGCAGGGGGCGAAGTGCAGTGGCTCATGCCTGTAATCTCAGCAA
TTTGGGAGGCCAAGGAAGGAGGAATGCTTGAGCCCAAGAGTTTTAGACCAGCCTGGGCAACACAGTAAGACCCTGTTTCTACAAAAAAATATAAAAAATA
  • refseq_P2RY6.F3 (forward primer)
  • refseq_P2RY6.R3 (reverse primer)
  • Single exon skipping

Frame 0

VSIGVKDWSFRVLGIRVLALLLASSVLWQVTSS
PQVSRFPHLLPLQTSARTLHATVSGTELTGSRGC
STSGNLLQHFTDCKRGTC*LLASLNIGNPPGQP
WNGTMAQARLWACHPPPVSTARTSSNCCCHLCI
RRCWRLACR*TSVSLPRSARPAGP*PARPCTP*T
LLWLTCYMPAPCPCSSTTMPKVITGPLATSPAA
WSASSSMPTCTAASSSSPASASSATWASATRWP
PGTNVGAAGLPG*CV*PCGWP*QPSACPQPSSLP
QASSVTALSAMTSARLPWPPTICPMAWLSLSSA
SCCPLLPCWPATVSWPAACAARMARQSLWPRSG
VARRPAWPWWWLLPLPSASCLFTSPRQPTWQCAR
RRASPALYWRPLQRPTKARGRLPVPTACWTPSS
STSPRRSSAGDHMSSYRNSQPNGRGRVAESSRS
WAAFIFAIVSGAPGAPPTPNHAEN*SSAQLGMEL
RSLTGPRSSPKTISSAPSLAQTLWAWRWTDLGL
ALERSQSAMESWGNHIKVLTKIQCDVYCHQGVC
SML*VTNEAGEGR*EGEVRASGKGHLSWVLRDDY
ELSGEE*YSIYLESLY*HLVLRPVLVLGP*KNQ
S*PWSIRAPNCWKYRLAQQHQGCDRGKHGWGEG
VHRQCP*PSPRSQERAL*GGSI*ARC*GLSGNLA
SRSGEHFNATQVCSMHIQLGQPRAGLCPGQTGR
GPEQRTHGLACVKAEKMGAVLQLPSRQGQELAR
CSRQWEANGGIKRRKLLRRGRGRSAVAHACNLSN
LGGQGRRNA*AQEF*TSLGNTVRPCFYKKI*KI

Frame 1

SALV*RTGASGFSGFESWPCCLPALCCGKLLPL
PRSLGFLICCLSRLLPEHCTRQFQAQN*LAAGA
APRVGICSSTSRTASEALANSWPP*T*ETHLGSH
GMGQWHRPGSGLATHHLCLPRELQATAAATCVF
GGAGGWPAAEHLCHYPDLHVPPGPDPHGRVHPK
PCSG*PAICLLPAPAHLQLCPR*SLALWRLRLPP
GPLPLLCQPARQHPLPHLHQLPALPGHLPPAGP
LAQTWGPPGCLASVCSRVAGRDNPVPAHSHLRC
HRHPA*PHCLL*PQPACPGHPLYALWHGSHCHRL
PAALCCPAGLLLSPGLPPVPPGWPGRACGPGAA
WQGGPHGRGGGCCLCHQLPAFSHHQDSLPGSAL
DAGRPLHCIGGLCSGLQRHAAVCQCQQRAGPHPL
LLHPEEVPPATT*APTETHSQMAEAGSLSPPGP
GQPSYLPLCPGHQEPHQPQTMRRIRVQLSWAWS
*DPSQDPEAHQKLFLQPLLWPRPCGHGDGQTWAW
LLRGPSQPWRAGETTLRCSQKYSVTCTVIKGYA
PCFESPMKRVREDERGR*ELLGRGI*AGF*GMI
MSSLERSDIPFI*KAFTDTLCSGLCWFWGPRRTS
PSPGPYGLPTAGSTDWHSNIRVVTEGSMAGGRG
YTDSAHDPVQGVRKELSEEGASEPDVEG*VGTW
QAEVGSTLMQPRYAPCISSWASLVLGSALGRQAE
GQSRGHMALRV*KLRKWELCFSYPPDKGKS*PD
APGSGKPMEGLSGGSFLEEAGGEVQWLMPVISA
IWEAKEGGMLEPKSFRPAWATQ*DPVSTKKYKK

Frame 2

QHWCKGLELQGSRDSSPGPAACQLCAVASYFLS
PGLSVSSSAASPDFCQNIARDSFRHRTDWQQGL
LHEWEFAPALHGLQARHLLTLGLPEHRKPTWAA
MEWDNGTGQALGLPPTTCVYRENFKQLLLPPVYS
AVLAAGLPLNICVITQICTSRRALTRTAVYTLN
LALADLLYACSLPLLIYNYAQGDHWPFGDFACR
LVRFLFYANLHGSILFLTCISFQRYLGICHPLAP
WHKRGGRRAAWLVCVAVWLAVTTQCLPTAIFAA
TGIQRNRTVCYDLSPPALATHYMPYGMALTVIG
FLLPFAALLACYCLLACRLCRQDGPAEPVAQERR
GKAARMAVVVAAAFAISFLPFHITKTAYLAVRS
TPGVPCTVLEAFAAAYKGTRPFASANSVLDPIL
FYFTQKKFRRRPHELLQKLTAKWQRQGR*VLQVL
GSLHICHCVRGTRSPTNPKPCGELEFSSAGHGV
KIPHRTQKLTKNYFFSPFSGPDPVGMEMDRPGP
GS*EVPVSHGELGKPH*GAHKNTV*RVLSSRGML
HALSHQ*SG*GKMRGGGESFWEGAFELGFEG*L
*ALWRGVIFHLFRKPLLTPCAQACVGSGALEEP
VLALVHTGSQLLEVQTGTATSGL*QREAWLGGGG
TQTVPMTQSKESGKSSLRREHLSQMLRAEWELG
KQKWGAL*CNPGMLHAYPAGPASCWALPWADRQ
RARAEDTWPCVCES*ENGSCASATLQTRARVSQM
LQAVGSQWRD*AAEAS*KRQGAKCSGSCL*SQQ
FGRPRKEECLSPRVLDQPGQHSKTLFLQKNIKN

Sequence NM_176796

CCAGGGACGCTGGGGCCGGAGCTGCTGCCGCCGTCTACACGGTCCCCTCATTTGACGGGTTCGCCTCCTAGCAGCGCCTGGGCGAGTGACATCTGGGCCG
GACCAGCTGGTGCTGCGCGGCGCAGGTCTCTCGGTTTCCTCATCTGCTGCCTCTCCAGACTTCTGCCAGAACATTGCACGCGACAGTTTCAGGCACAGAA
CTGACTGGCAGCAGGGGCTGCTCCACGAGTGGGAATTTGCTCCAGCACTTCACGGACTGCAAGCGAGGCACTTGCTAACTCTTGGATAACAAGACCTCTG
CCAGAAGAACCATGGCTTTGGAAGGCGGAGTTCAGGCTGAGGAGATGGGTGCGGTCCTCAGTGAGCCCCTG
CCTCCCTGAACATAGGAAACCCACCTGGG
CAGCCATGGAATGGGACAATGGCACAGGCCAGGCTCTGGGCTTGCCACCCACCACCTGTGTCTACCGCGAGAACTTCAAGCAACTGCTGCTGCCACCTGT
GTATTCGGCGGTGCTGGCGGCTGGCCTGCCGCTGAACATCTGTGTCATTACCCAGATCTGCACGTCCCGCCGGGCCCTGACCCGCACGGCCGTGTACACC
CTAAACCTTGCTCTGGCTGACCTGCTATATGCCTGCTCCCTGCCCCTGCTCATCTACAACTATGCCCAAGGTGATCACTGGCCCTTTGGCGACTTCGCCT
GCCGCCTGGTCCGCTTCCTCTTCTATGCCAACCTGCACGGCAGCATCCTCTTCCTCACCTGCATCAGCTTCCAGCGCTACCTGGGCATCTGCCACCCGCT
GGCCCCCTGGCACAAACGTGGGGGCCGCCGGGCTGCCTGGCTAGTGTGTGTAGCCGTGTGGCTGGCCGTGACAACCCAGTGCCTGCCCACAGCCATCTTC
GCTGCCACAGGCATCCAGCGTAACCGCACTGTCTGCTATGACCTCAGCCCGCCTGCCCTGGCCACCCACTATATGCCCTATGGCATGGCTCTCACTGTCA
TCGGCTTCCTGCTGCCCTTTGCTGCCCTGCTGGCCTGCTACTGTCTCCTGGCCTGCCGCCTGTGCCGCCAGGATGGCCCGGCAGAGCCTGTGGCCCAGGA
GCGGCGTGGCAAGGCGGCCCGCATGGCCGTGGTGGTGGCTGCTGCCTTTGCCATCAGCTTCCTGCCTTTTCACATCACCAAGACAGCCTACCTGGCAGTG
CGCTCGACGCCGGGCGTCCCCTGCACTGTATTGGAGGCCTTTGCAGCGGCCTACAAAGGCACGCGGCCGTTTGCCAGTGCCAACAGCGTGCTGGACCCCA
TCCTCTTCTACTTCACCCAGAAGAAGTTCCGCCGGCGACCACATGAGCTCCTACAGAAACTCACAGCCAAATGGCAGAGGCAGGGTCGCTGAGTCCTCCA
GGTCCTGGGCAGCCTTCATATTTGCCATTGTGTCCGGGGCACCAGGAGCCCCACCAACCCCAAACCATGCGGAGAATTAGAGTTCAGCTCAGCTGGGCAT
GGAGTTAAGATCCCTCACAGGACCCAGAAGCTCACCAAAAACTATTTCTTCAGCCCCTTCTCTGGCCCAGACCCTGTGGGCATGGAGATGGACAGACCTG
GGCCTGGCTCTTGAGAGGTCCCAGTCAGCCATGGAGAGCTGGGGAAACCACATTAAGGTGCTCACAAAAATACAGTGTGACGTGTACTGTCATCAAGGGG
TATGCTCCATGCTTTGAGTCACCAATGAAGCGGGTGAGGGAAGATGAGAGGGGGAGGTGAGAGCTTCTGGGAAGGGGCATTTGAGCTGGGTTTTGAGGGA
TGATTATGAGCTCTCTGGAGAGGAGTGATATTCCATTTATTTAGAAAGCCTTTACTGACACCTTGTGCTCAGGCCTGTGTTGGTTCTGGGGCCCTAGAAG
AACCAGTCCTAGCCCTGGTCCATACGGGCTCCCAACTGCTGGAAGTACAGACTGGCACAGCAACATCAGGGTTGTGACAGAGGGAAGCATGGCTGGGGGG
AGGGGGTACACAGACAGTGCCCATGACCCAGTCCAAGGAGTCAGGAAAGAGCTCTCTGAGGAGGGAGCATCTGAGCCAGATGTTGAGGGCTGAGTGGGAA
CTTGGCAAGCAGAAGTGGGGAGCACTTTAATGCAACCCAGGTATGCTCCATGCATATCCAGCTGGGCCAGCCTCGTGCTGGGCTCTGCCCTGGGCAGACA
GGCAGAGGGCCAGAGCAGAGGACACATGGCCTTGCGTGTGTGAAAGCTGAGAAAATGGGAGCTGTGCTTCAGCTACCCTCCAGACAAGGGCAAGAGTTAG
CCAGATGCTCCAGGCAGTGGGAAGCCAATGGAGGGATTAAGCGGCGGAAGCTTCTTAGAAGAGGCAGGGGGCGAAGTGCAGTGGCTCATGCCTGTAATCT
CAGCAATTTGGGAGGCCAAGGAAGGAGGAATGCTTGAGCCCAAGAGTTTTAGACCAGCCTGGGCAACACAGTAAGACCCTGTTTCTACAAAAAAATATAA
AAAATA
  • refseq_P2RY6.F3 (forward primer)
  • refseq_P2RY6.R3 (reverse primer)
  • Single exon skipping

Frame 0

PGTLGPELLPPSTRSPHLTGSPPSSAWASDIWA
GPAGAARRRSLGFLICCLSRLLPEHCTRQFQAQN
*LAAGAAPRVGICSSTSRTASEALANSWITRPL
PEEPWLWKAEFRLRRWVRSSVSPC
LPEHRKPTW
AAMEWDNGTGQALGLPPTTCVYRENFKQLLLPPV
YSAVLAAGLPLNICVITQICTSRRALTRTAVYT
LNLALADLLYACSLPLLIYNYAQGDHWPFGDFA
CRLVRFLFYANLHGSILFLTCISFQRYLGICHPL
APWHKRGGRRAAWLVCVAVWLAVTTQCLPTAIF
AATGIQRNRTVCYDLSPPALATHYMPYGMALTV
IGFLLPFAALLACYCLLACRLCRQDGPAEPVAQE
RRGKAARMAVVVAAAFAISFLPFHITKTAYLAV
RSTPGVPCTVLEAFAAAYKGTRPFASANSVLDP
ILFYFTQKKFRRRPHELLQKLTAKWQRQGR*VLQ
VLGSLHICHCVRGTRSPTNPKPCGELEFSSAGH
GVKIPHRTQKLTKNYFFSPFSGPDPVGMEMDRP
GPGS*EVPVSHGELGKPH*GAHKNTV*RVLSSRG
MLHALSHQ*SG*GKMRGGGESFWEGAFELGFEG
*L*ALWRGVIFHLFRKPLLTPCAQACVGSGALE
EPVLALVHTGSQLLEVQTGTATSGL*QREAWLGG
GGTQTVPMTQSKESGKSSLRREHLSQMLRAEWE
LGKQKWGAL*CNPGMLHAYPAGPASCWALPWAD
RQRARAEDTWPCVCES*ENGSCASATLQTRARVS
QMLQAVGSQWRD*AAEAS*KRQGAKCSGSCL*S
QQFGRPRKEECLSPRVLDQPGQHSKTLFLQKNI
KN

Frame 1

QGRWGRSCCRRLHGPLI*RVRLLAAPGRVTSGP
DQLVLRGAGLSVSSSAASPDFCQNIARDSFRHR
TDWQQGLLHEWEFAPALHGLQARHLLTLG*QDLC
QKNHGFGRRSSG*GDGCGPQ*AP
ASLNIGNPPG
QPWNGTMAQARLWACHPPPVSTARTSSNCCCHL
CIRRCWRLACR*TSVSLPRSARPAGP*PARPCTP
*TLLWLTCYMPAPCPCSSTTMPKVITGPLATSP
AAWSASSSMPTCTAASSSSPASASSATWASATR
WPPGTNVGAAGLPG*CV*PCGWP*QPSACPQPSS
LPQASSVTALSAMTSARLPWPPTICPMAWLSLS
SASCCPLLPCWPATVSWPAACAARMARQSLWPR
SGVARRPAWPWWWLLPLPSASCLFTSPRQPTWQC
ARRRASPALYWRPLQRPTKARGRLPVPTACWTP
SSSTSPRRSSAGDHMSSYRNSQPNGRGRVAESS
RSWAAFIFAIVSGAPGAPPTPNHAEN*SSAQLGM
ELRSLTGPRSSPKTISSAPSLAQTLWAWRWTDL
GLALERSQSAMESWGNHIKVLTKIQCDVYCHQG
VCSML*VTNEAGEGR*EGEVRASGKGHLSWVLRD
DYELSGEE*YSIYLESLY*HLVLRPVLVLGP*K
NQS*PWSIRAPNCWKYRLAQQHQGCDRGKHGWG
EGVHRQCP*PSPRSQERAL*GGSI*ARC*GLSGN
LASRSGEHFNATQVCSMHIQLGQPRAGLCPGQT
GRGPEQRTHGLACVKAEKMGAVLQLPSRQGQEL
ARCSRQWEANGGIKRRKLLRRGRGRSAVAHACNL
SNLGGQGRRNA*AQEF*TSLGNTVRPCFYKKI*
KI

Frame 2

RDAGAGAAAAVYTVPSFDGFAS*QRLGE*HLGR
TSWCCAAQVSRFPHLLPLQTSARTLHATVSGTE
LTGSRGCSTSGNLLQHFTDCKRGTC*LLDNKTS
ARRTMALEGGVQAEEMGAVLSEPL
PP*T*ETHLG
SHGMGQWHRPGSGLATHHLCLPRELQATAAATC
VFGGAGGWPAAEHLCHYPDLHVPPGPDPHGRVH
PKPCSG*PAICLLPAPAHLQLCPR*SLALWRLRL
PPGPLPLLCQPARQHPLPHLHQLPALPGHLPPA
GPLAQTWGPPGCLASVCSRVAGRDNPVPAHSHL
RCHRHPA*PHCLL*PQPACPGHPLYALWHGSHCH
RLPAALCCPAGLLLSPGLPPVPPGWPGRACGPG
AAWQGGPHGRGGGCCLCHQLPAFSHHQDSLPGS
ALDAGRPLHCIGGLCSGLQRHAAVCQCQQRAGPH
PLLLHPEEVPPATT*APTETHSQMAEAGSLSPP
GPGQPSYLPLCPGHQEPHQPQTMRRIRVQLSWA
WS*DPSQDPEAHQKLFLQPLLWPRPCGHGDGQTW
AWLLRGPSQPWRAGETTLRCSQKYSVTCTVIKG
YAPCFESPMKRVREDERGR*ELLGRGI*AGF*G
MIMSSLERSDIPFI*KAFTDTLCSGLCWFWGPRR
TSPSPGPYGLPTAGSTDWHSNIRVVTEGSMAGG
RGYTDSAHDPVQGVRKELSEEGASEPDVEG*VG
TWQAEVGSTLMQPRYAPCISSWASLVLGSALGRQ
AEGQSRGHMALRV*KLRKWELCFSYPPDKGKS*
PDAPGSGKPMEGLSGGSFLEEAGGEVQWLMPVI
SAIWEAKEGGMLEPKSFRPAWATQ*DPVSTKKYK
K

Sequence NM_176798

AAGGACAGAGGAGGGGCCCTTCCTGTCAGCTGGCTGGGAGCAGAGGTGGCTTTGTCTTTTCGGAAGAACTGGTTCTGTGGAATTTGTGCTTATTTCCCAT
CAAGGATCAAGGACCTGCTCTGGGGCTACCTCAGGGCCCCACAGGATGAGGGGCTGGTTTTCAGATGAGTTTTCTGCTTGCCTGTCATCTGGATAGTGTC
TAAAAATTTGCAAACTGCCTTCTTGTCAGTGTCTTGCTCATTCTTCATGACACTCCTGATATGTCTCTCGGTTTCCTCATCTGCTGCCTCTCCAGACTTC
TGCCAGAACATTGCACGCGACAGTTTCAGGCACAGAACTGACTGGCAGCAGGGGCTGCTCCACGAGTGGGAATTTGCTCCAGCACTTCACGGACTGCAAG
CGAGGCACTTGCTAACTCTTGGATAACAAGACCTCTGCCAGAAGAACCATGGCTTTGGAAGGCGGAGTTCAGGCTGAGGAGATGGGTGCGGTCCTCAGTG
AGCCCCTG
CCTCCCTGAACATAGGAAACCCACCTGGGCAGCCATGGAATGGGACAATGGCACAGGCCAGGCTCTGGGCTTGCCACCCACCACCTGTGTCT
ACCGCGAGAACTTCAAGCAACTGCTGCTGCCACCTGTGTATTCGGCGGTGCTGGCGGCTGGCCTGCCGCTGAACATCTGTGTCATTACCCAGATCTGCAC
GTCCCGCCGGGCCCTGACCCGCACGGCCGTGTACACCCTAAACCTTGCTCTGGCTGACCTGCTATATGCCTGCTCCCTGCCCCTGCTCATCTACAACTAT
GCCCAAGGTGATCACTGGCCCTTTGGCGACTTCGCCTGCCGCCTGGTCCGCTTCCTCTTCTATGCCAACCTGCACGGCAGCATCCTCTTCCTCACCTGCA
TCAGCTTCCAGCGCTACCTGGGCATCTGCCACCCGCTGGCCCCCTGGCACAAACGTGGGGGCCGCCGGGCTGCCTGGCTAGTGTGTGTAGCCGTGTGGCT
GGCCGTGACAACCCAGTGCCTGCCCACAGCCATCTTCGCTGCCACAGGCATCCAGCGTAACCGCACTGTCTGCTATGACCTCAGCCCGCCTGCCCTGGCC
ACCCACTATATGCCCTATGGCATGGCTCTCACTGTCATCGGCTTCCTGCTGCCCTTTGCTGCCCTGCTGGCCTGCTACTGTCTCCTGGCCTGCCGCCTGT
GCCGCCAGGATGGCCCGGCAGAGCCTGTGGCCCAGGAGCGGCGTGGCAAGGCGGCCCGCATGGCCGTGGTGGTGGCTGCTGCCTTTGCCATCAGCTTCCT
GCCTTTTCACATCACCAAGACAGCCTACCTGGCAGTGCGCTCGACGCCGGGCGTCCCCTGCACTGTATTGGAGGCCTTTGCAGCGGCCTACAAAGGCACG
CGGCCGTTTGCCAGTGCCAACAGCGTGCTGGACCCCATCCTCTTCTACTTCACCCAGAAGAAGTTCCGCCGGCGACCACATGAGCTCCTACAGAAACTCA
CAGCCAAATGGCAGAGGCAGGGTCGCTGAGTCCTCCAGGTCCTGGGCAGCCTTCATATTTGCCATTGTGTCCGGGGCACCAGGAGCCCCACCAACCCCAA
ACCATGCGGAGAATTAGAGTTCAGCTCAGCTGGGCATGGAGTTAAGATCCCTCACAGGACCCAGAAGCTCACCAAAAACTATTTCTTCAGCCCCTTCTCT
GGCCCAGACCCTGTGGGCATGGAGATGGACAGACCTGGGCCTGGCTCTTGAGAGGTCCCAGTCAGCCATGGAGAGCTGGGGAAACCACATTAAGGTGCTC
ACAAAAATACAGTGTGACGTGTACTGTCATCAAGGGGTATGCTCCATGCTTTGAGTCACCAATGAAGCGGGTGAGGGAAGATGAGAGGGGGAGGTGAGAG
CTTCTGGGAAGGGGCATTTGAGCTGGGTTTTGAGGGATGATTATGAGCTCTCTGGAGAGGAGTGATATTCCATTTATTTAGAAAGCCTTTACTGACACCT
TGTGCTCAGGCCTGTGTTGGTTCTGGGGCCCTAGAAGAACCAGTCCTAGCCCTGGTCCATACGGGCTCCCAACTGCTGGAAGTACAGACTGGCACAGCAA
CATCAGGGTTGTGACAGAGGGAAGCATGGCTGGGGGGAGGGGGTACACAGACAGTGCCCATGACCCAGTCCAAGGAGTCAGGAAAGAGCTCTCTGAGGAG
GGAGCATCTGAGCCAGATGTTGAGGGCTGAGTGGGAACTTGGCAAGCAGAAGTGGGGAGCACTTTAATGCAACCCAGGTATGCTCCATGCATATCCAGCT
GGGCCAGCCTCGTGCTGGGCTCTGCCCTGGGCAGACAGGCAGAGGGCCAGAGCAGAGGACACATGGCCTTGCGTGTGTGAAAGCTGAGAAAATGGGAGCT
GTGCTTCAGCTACCCTCCAGACAAGGGCAAGAGTTAGCCAGATGCTCCAGGCAGTGGGAAGCCAATGGAGGGATTAAGCGGCGGAAGCTTCTTAGAAGAG
GCAGGGGGCGAAGTGCAGTGGCTCATGCCTGTAATCTCAGCAATTTGGGAGGCCAAGGAAGGAGGAATGCTTGAGCCCAAGAGTTTTAGACCAGCCTGGG
CAACACAGTAAGACCCTGTTTCTACAAAAAAATATAAAAAATA
  • refseq_P2RY6.F3 (forward primer)
  • refseq_P2RY6.R3 (reverse primer)
  • Single exon skipping

Frame 0

KDRGGALPVSWLGAEVALSFRKNWFCGICAYFP
SRIKDLLWGYLRAPQDEGLVFR*VFCLPVIWIVS
KNLQTAFLSVSCSFFMTLLICLSVSSSAASPDF
CQNIARDSFRHRTDWQQGLLHEWEFAPALHGLQ
ARHLLTLG*QDLCQKNHGFGRRSSG*GDGCGPQ*
AP
ASLNIGNPPGQPWNGTMAQARLWACHPPPVS
TARTSSNCCCHLCIRRCWRLACR*TSVSLPRSA
RPAGP*PARPCTP*TLLWLTCYMPAPCPCSSTTM
PKVITGPLATSPAAWSASSSMPTCTAASSSSPA
SASSATWASATRWPPGTNVGAAGLPG*CV*PCG
WP*QPSACPQPSSLPQASSVTALSAMTSARLPWP
PTICPMAWLSLSSASCCPLLPCWPATVSWPAAC
AARMARQSLWPRSGVARRPAWPWWWLLPLPSAS
CLFTSPRQPTWQCARRRASPALYWRPLQRPTKAR
GRLPVPTACWTPSSSTSPRRSSAGDHMSSYRNS
QPNGRGRVAESSRSWAAFIFAIVSGAPGAPPTP
NHAEN*SSAQLGMELRSLTGPRSSPKTISSAPSL
AQTLWAWRWTDLGLALERSQSAMESWGNHIKVL
TKIQCDVYCHQGVCSML*VTNEAGEGR*EGEVR
ASGKGHLSWVLRDDYELSGEE*YSIYLESLY*HL
VLRPVLVLGP*KNQS*PWSIRAPNCWKYRLAQQ
HQGCDRGKHGWGEGVHRQCP*PSPRSQERAL*G
GSI*ARC*GLSGNLASRSGEHFNATQVCSMHIQL
GQPRAGLCPGQTGRGPEQRTHGLACVKAEKMGA
VLQLPSRQGQELARCSRQWEANGGIKRRKLLRR
GRGRSAVAHACNLSNLGGQGRRNA*AQEF*TSLG
NTVRPCFYKKI*KI

Frame 1

RTEEGPFLSAGWEQRWLCLFGRTGSVEFVLISH
QGSRTCSGATSGPHRMRGWFSDEFSACLSSG*C
LKICKLPSCQCLAHSS*HS*YVSRFPHLLPLQTS
ARTLHATVSGTELTGSRGCSTSGNLLQHFTDCK
RGTC*LLDNKTSARRTMALEGGVQAEEMGAVLS
EPL
PP*T*ETHLGSHGMGQWHRPGSGLATHHLCL
PRELQATAAATCVFGGAGGWPAAEHLCHYPDLH
VPPGPDPHGRVHPKPCSG*PAICLLPAPAHLQL
CPR*SLALWRLRLPPGPLPLLCQPARQHPLPHLH
QLPALPGHLPPAGPLAQTWGPPGCLASVCSRVA
GRDNPVPAHSHLRCHRHPA*PHCLL*PQPACPG
HPLYALWHGSHCHRLPAALCCPAGLLLSPGLPPV
PPGWPGRACGPGAAWQGGPHGRGGGCCLCHQLP
AFSHHQDSLPGSALDAGRPLHCIGGLCSGLQRH
AAVCQCQQRAGPHPLLLHPEEVPPATT*APTETH
SQMAEAGSLSPPGPGQPSYLPLCPGHQEPHQPQ
TMRRIRVQLSWAWS*DPSQDPEAHQKLFLQPLL
WPRPCGHGDGQTWAWLLRGPSQPWRAGETTLRCS
QKYSVTCTVIKGYAPCFESPMKRVREDERGR*E
LLGRGI*AGF*GMIMSSLERSDIPFI*KAFTDT
LCSGLCWFWGPRRTSPSPGPYGLPTAGSTDWHSN
IRVVTEGSMAGGRGYTDSAHDPVQGVRKELSEE
GASEPDVEG*VGTWQAEVGSTLMQPRYAPCISS
WASLVLGSALGRQAEGQSRGHMALRV*KLRKWEL
CFSYPPDKGKS*PDAPGSGKPMEGLSGGSFLEE
AGGEVQWLMPVISAIWEAKEGGMLEPKSFRPAW
ATQ*DPVSTKKYKK

Frame 2

GQRRGPSCQLAGSRGGFVFSEELVLWNLCLFPI
KDQGPALGLPQGPTG*GAGFQMSFLLACHLDSV
*KFANCLLVSVLLILHDTPDMSLGFLICCLSRL
LPEHCTRQFQAQN*LAAGAAPRVGICSSTSRTAS
EALANSWITRPLPEEPWLWKAEFRLRRWVRSSV
SPC
LPEHRKPTWAAMEWDNGTGQALGLPPTTCV
YRENFKQLLLPPVYSAVLAAGLPLNICVITQICT
SRRALTRTAVYTLNLALADLLYACSLPLLIYNY
AQGDHWPFGDFACRLVRFLFYANLHGSILFLTC
ISFQRYLGICHPLAPWHKRGGRRAAWLVCVAVWL
AVTTQCLPTAIFAATGIQRNRTVCYDLSPPALA
THYMPYGMALTVIGFLLPFAALLACYCLLACRL
CRQDGPAEPVAQERRGKAARMAVVVAAAFAISFL
PFHITKTAYLAVRSTPGVPCTVLEAFAAAYKGT
RPFASANSVLDPILFYFTQKKFRRRPHELLQKL
TAKWQRQGR*VLQVLGSLHICHCVRGTRSPTNPK
PCGELEFSSAGHGVKIPHRTQKLTKNYFFSPFS
GPDPVGMEMDRPGPGS*EVPVSHGELGKPH*GA
HKNTV*RVLSSRGMLHALSHQ*SG*GKMRGGGES
FWEGAFELGFEG*L*ALWRGVIFHLFRKPLLTP
CAQACVGSGALEEPVLALVHTGSQLLEVQTGTA
TSGL*QREAWLGGGGTQTVPMTQSKESGKSSLRR
EHLSQMLRAEWELGKQKWGAL*CNPGMLHAYPA
GPASCWALPWADRQRARAEDTWPCVCES*ENGS
CASATLQTRARVSQMLQAVGSQWRD*AAEAS*KR
QGAKCSGSCL*SQQFGRPRKEECLSPRVLDQPG
QHSKTLFLQKNIKN

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