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Sequence NM_130842
GCCTCCCGCCGCCTCCCGCGCGGCCATGGACTGAGCGCCGCCGGCCAGGCCGCGGGGATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCCCTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGATGGAGTGTTTGGAAGGTGCCAGAAGGTTCC
GGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGCCTGCGCGTGGCGTTGCAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGAT
GACTATACTCAGTATGTGATGGACCAGGAACTTGCAGACCTCCCGAAAACCTACCTGAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACA
GCGTTGGCAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCTGCCCTGGCCAACGCCCTCCGACGCCACCTGCCCTTCCTGGAGGCCCTGTCCCAGGCCCC
AGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCCCCCGCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCAC
ACGTCTGCGCTGACCTACCCTCCCGGGTCCCGGACCCAGCTCCGCGAGGACCTCCTGCCGCGGACCCTCGGCCAGCTCCAGCCAGATGAGCTCAGCCCTA
AGGTGGACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGTGCCTATGCTGCCCAGAGGCCCCCAGCTCCCCCCGGGGAGGGCAGCCTGGAGCC
ACAGTACCTTCTGCGTGCACCCTCAAGAATGCCCAGGCCTTTGCTGGCACCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGACCCC
TCCAGCACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGCAGAGGCAGCCGGCTGAGGTGAGGGGCCTGAGTGGCCTGGAGCTGGACGGCA
TGGCTGAGCTGATGGCTGGCCTGATGCAAGGCGTGGACCATGGAGTAGCTCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAACAGGCGGA
TGGCCCCAAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGAGTGCAGGACGACGATGATAGACTTTACCAAGAGGTCCATCGTCTGAGTGCCACA
CTCGGGGGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGCCCTCCCCTTTGCAAGGCCCCTCGACATGGAGAGGAAGAAGTCCGAGCACCCTG
AGTCTTCCCTGTCTTCAGAAGAGGAGACTGCCGGAGTGGAGAACGTCAAGAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAGCCGCATTCGGAGCC
CGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCCTCGAAGGAGGAGCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGC
CTGCAATTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACATCGTGACAGACAGAGACCCCCTGCGCCCCGAGGAAGGAAGGCGGCTGGTGGAGG
ACGTCGCCCGCCTCCTGCAGGTGCCCAGCAGTGCGTTCGCTGACGTGGAGGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCCAATGTCCAAAACGT
GACCACTGAGGATGTGGAGAAGGCCACAGTTGACAACAAAGACAAACTGGAGGAAACCTCTGGACTGAAAATTCTTCAAACCGGAGTCGGGTCGAAAAGC
AAACTCAAGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCACCAAGTTCATCGCGCTCACCCTGGTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGG
CCTCTGGCCTCATCTACTGCCTCCGCCATAGCTCTCAGCACAGGCTGAAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCAGATGCCACTGCCGC
CTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCGGCCACCAGACCGACCTGAGGGCCCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGC
GACGGGCCGATCCCCAGCCCCTCCGCACGCAGCAGCGCCTCATCCTGGTCCGAGGAGCCTGTGCAGTCCAACATGGACATCTCCACCGGCCACATGATCC
TGTCCTACATGGAGGACCACCTGAAGAACAAGAACCGGCTGGAGAAGGAGTGGGAAGCGCTGTGCGCCTACCAGGCGGAGCCCAACAGCTCGTTCGTGGC
CCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCTGGCCGTGCTGACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCA
GACTACATCAACGCTAGCCCCATCATGGATCACGACCCGAGGAACCCCGCGTACATCGCCACCCAGGGACCGCTGCCCGCCACCGTGGCTGACTTTTGGC
AGATGGTGTGGGAGAGCGGCTGCGTGGTGATCGTCATGCTGACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGGCCGGATGAAGGCTC
CAATCTCTACCACATCTATGAGGTGAACCTGGTCTCCGAGCACATCTGGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAAC
GAGACGCGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGAGGAGTCCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACA
AGTGCTACAGGGGCCGTTCTTGTCCAATAATTGTTCATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATGGTTCTCAACAAGAT
GGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGGAGCACTTGAGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCG
CTGACAGCCGTGGCTGAGGAGGTGAACGCCATCCTCAAGGCCCTTCCCCAGTGAGCGGCAGCCTCAGGGGCCTCAGGGGAGCCCCCACCCCACGGATGTT
GTCAGGAATCATGATCTGACTTTAATTGTGTGTCTTCTATTATAACTGCATAGTAATAGGGCCCTTAGCTCTCCCGTAGTCAGCGCAGTTTAGCAGTTAA
AAGTGTATTTTTGTTTAATCAAACAATAATAAAGAGAGATTTGTGGAAAAATCCAGTTACGGGTGGAGGGGAATCGGTTCATCAATTTTCACTTGCTTAA
AAAAAATACTTTTTCTTAAAGCACCCGTTCACCTTCTTGGTTGAAGTTGTGTTAACAATGCAGTAGCCAGCACGTTCGAGGCGGTTTCCAGGAAGAGTGT
GCTTGTCATCTGCCACTTTCGGGAGGGTGGATCCACTGTGCAGGAGTGGCCGGGGAAGCTGGCAGCACTCAGTGAGGCCGCCCGGCACACAAGGCACGTT
TGGCATTTCTCTTTGAGAGAGTTTATCATTGGGAGAAGCCGCAGGGACAGAACTGAACGTCCTGCAGCTTCGGGGCAAGTGAGACAATCACAGCTCCTCG
CTGCGTCTCCATCAACACTGCGCCGGGTACCATGGACGGCCCCGTCAGCCACACCTGTCAGCCCAAGCAGAGTGATTCAGGGGCTCCCCGGGGGCAGACA
CCTGTGCACCCCATGAGTAGTGCCCACTTGAGGCTGGCACTCCCCTGACCTCACCTTTGCAAAGTTACAGATGCACCCCAACATTGAGATGTGTTTTTAA
TGTTAAAATATTGATTTCTACGTTATGAAAACAGATGCCCCCGTGAATGCTTACCTGTGAGATAACCACAACCAGGAAGAACAAATCTGGGCATTGAGCA
AGCTATGAGGGTCCCCGGGAGCACACGAACCCTGCCAGGCCCCCGCTGGCTCCTCCAGGCACGTCCCGGACCTGTGGGGCCCCAGAGAGGGGACATTTCC
CTCCTGGGAGAGAAGGAGATCAGGGCAACTCGGAGAGGGCTGCGAGCATTTCCCTCCCGGGAGAGGAGATCAGGGCGACCTGCACGCACTGCGTAGAGCC
TGGAAGGGAAGTGAGAAACCAGCCGACCGGCCCTGCCCCTCTTCCCGGGATCACTTAATGAACCACGTGTTTTGACATCATGTAAACCTAAGCACGTAGA
GATGATTCGGATTTGACAAAATAACATTTGAGTATCCGATTCGCCATCACCCCCTACCCCAGAAATAGGACAATTCACTTCATTGACCAAGATGATCACA
TGGAAGGCGGCACAGAGGCAGCTGTGTGGGCTGCAGATTTCCTGTGTGGGGTTCAGCGTAGAAAACGCACCTCCATCCCGCCCTTCCCACAGCATTCCTC
CATCTTAGATAGATGGTACTCTCCAAAGGCCCTACCAGAGGGAACACGGCCTACTGAGCGGACAGAATGATGCCAAAATATTGCTTATGTCTCTACATGG
TATTGTAATGAATATCTGCTTTAATATAGCTATCATTTCTTTTCCAAAATTACTTCTCTCTATCTGGAATTTAATTAATCGAAATGAATTTATCTGAATA
TAGGAAGCATATGCCTACTTGTAATTTCTAACTCCTTATGTTTGAAGAGAAACCTCCGGTGTGAGATATACAAATATATTTAATTGTGTCATATTAAACT
TCTGATT
- refseq_PTPRN2.F1 (forward primer)
- refseq_PTPRN2.R1 (reverse primer)
- Single exon skipping
Frame 0
ASRRLPRGHGLSAAGQAAGMGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLDGVFGRCQKVP
AMDFYRYEVSPVALQRLRVALQKLSGTGFTWQD
DYTQYVMDQELADLPKTYLRRPEASSPARPSKH
SVGSERRYSREGGAALANALRRHLPFLEALSQAP
ASDVLARTHTAQDRPPAEGDDRFSESILTYVAH
TSALTYPPGSRTQLREDLLPRTLGQLQPDELSP
KVDSGVDRHHLMAALSAYAAQRPPAPPGEGSLEP
QYLLRAPSRMPRPLLAPAAPQKWPSPLGDSEDP
SSTGDGARIHTLLKDLQRQPAEVRGLSGLELDG
MAELMAGLMQGVDHGVARGSPGRAALGESGEQAD
GPKATLRGDSFPDDGVQDDDDRLYQEVHRLSAT
LGGLLQDHGSRLLPGALPFARPLDMERKKSEHP
ESSLSSEEETAGVENVKSQTYSKDLLGQQPHSEP
GAAAFGELQNQMPGPSKEEQSLPAGAQEALSDG
LQLEVQPSEEEARGYIVTDRDPLRPEEGRRLVE
DVARLLQVPSSAFADVEVLGPAVTFKVSANVQNV
TTEDVEKATVDNKDKLEETSGLKILQTGVGSKS
KLKFLPPQAEQEDSTKFIALTLVSLACILGVLL
ASGLIYCLRHSSQHRLKEKLSGLGGDPGADATAA
YQELCRQRMATRPPDRPEGPHTSRISSVSSQFS
DGPIPSPSARSSASSWSEEPVQSNMDISTGHMI
LSYMEDHLKNKNRLEKEWEALCAYQAEPNSSFVA
QREENVPKNRSLAVLTYDHSRVLLKAENSHSHS
DYINASPIMDHDPRNPAYIATQGPLPATVADFW
QMVWESGCVVIVMLTPLAENGVRQCYHYWPDEGS
NLYHIYEVNLVSEHIWCEDFLVRSFYLKNLQTN
ETRTVTQFHFLSWYDRGVPSSSRSLLDFRRKVN
KCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKM
AKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFA
LTAVAEEVNAILKALPQ*AAASGASGEPPPHGC
CQES*SDFNCVSSIITA***GP*LSRSQRSLAVK
SVFLFNQTIIKRDLWKNPVTGGGESVHQFSLA*
KKYFFLKHPFTFLVEVVLTMQ*PARSRRFPGRV
CLSSATFGRVDPLCRSGRGSWQHSVRPPGTQGTF
GISL*ESLSLGEAAGTELNVLQLRGK*DNHSSS
LRLHQHCAGYHGRPRQPHLSAQAE*FRGSPGAD
TCAPHE*CPLEAGTPLTSPLQSYRCTPTLRCVFN
VKILISTL*KQMPP*MLTCEITTTRKNKSGH*A
SYEGPREHTNPARPPLAPPGTSRTCGAPERGHF
PPGREGDQGNSERAASISLPGEEIRATCTHCVEP
GREVRNQPTGPAPLPGIT**TTCFDIM*T*ARR
DDSDLTK*HLSIRFAITPYPRNRTIHFIDQDDH
MEGGTEAAVWAADFLCGVQRRKRTSIPPFPQHSS
ILDRWYSPKALPEGTRPTERTE*CQNIAYVSTW
YCNEYLL*YSYHFFSKITSLYLEFN*SK*IYLN
IGSICLLVISNSLCLKRNLRCEIYKYI*LCHIKL
LI
Frame 1
PPAASRAAMD*APPARPRGWGRRSRCCCCYCCCCRHASCLPPLRPSPAAGSSRGVWMECLEGARRF
RQWTFTATRCRPWPCSACAWRCRSFPAQVSRGRM
TILSM*WTRNLQTSRKPT*GVLKHPAQPGPQNT
ALAARGGTVGRAVLPWPTPSDATCPSWRPCPRP
QPQTCSPGPIRRRTDPPLRVMTASPRAS*PMWPT
RLR*PTLPGPGPSSARTSCRGPSASSSQMSSAL
RWTVVWTDTI*WRPSVPMLPRGPQLPPGRAAWS
HSTFCVHPQECPGLCWHQPPPRSGLHLWEIPKTP
PAQAMEHGFIPS*RTCRGSRLR*GA*VAWSWTA
WLS*WLA*CKAWTME*LEAALGERPWESLENRR
MAPRPPSVETAFQMTECRTTMIDFTKRSIV*VPH
SGASCRTTGLDSYLEPSPLQGPSTWRGRSPSTL
SLPCLQKRRLPEWRTSRARRIPKICWGSSRIRS
PGPLRLGSSKTRCLGPRRRSRAFQRVLRRPSATA
CNWRSSLPRKRRGATS*QTETPCAPRKEGGWWR
TSPASCRCPAVRSLTWRFSDQQ*PSK*APMSKT
*PLRMWRRPQLTTKTNWRKPLD*KFFKPESGRKA
NSSSCLLRRSKKTPPSSSRSPWSPSPASWASSW
PLASSTASAIALSTG*RRSSRD*GATQVQMPLP
PTRSCAASVWPRGHQTDLRARTRHASAASHPSSA
TGRSPAPPHAAAPHPGPRSLCSPTWTSPPAT*S
CPTWRTT*RTRTGWRRSGKRCAPTRRSPTARSW
PRGRRTCPRTAPWPC*PMTTPGSC*RRRTATATQ
TTSTLAPSWITTRGTPRTSPPRDRCPPPWLTFG
RWCGRAAAW*SSC*HPSRRTASGSATTTGRMKA
PISTTSMR*TWSPSTSGVRTSW*GASI*RTCRPT
RRAP*RSSTS*VGMTEESLPPQGPSWTSAEK*T
SATGAVLVQ*LFIAVTVQAGAAPTS*STWFSTR
WPKVLKRLISQRPWST*GTRDPAWSRRRSSLSSR
*QPWLRR*TPSSRPFPSERQPQGPQGSPHPTDV
VRNHDLTLIVCLLL*LHSNRALSSPVVSAV*QL
KVYFCLIKQ**REICGKIQLRVEGNRFINFHLLK
KNTFS*STRSPSWLKLC*QCSSQHVRGGFQEEC
ACHLPLSGGWIHCAGVAGEAGSTQ*GRPAHKAR
LAFLFERVYHWEKPQGQN*TSCSFGASETITAPR
CVSINTAPGTMDGPVSHTCQPKQSDSGAPRGQT
PVHPMSSAHLRLALP*PHLCKVTDAPQH*DVFL
MLKY*FLRYENRCPRECLPVR*PQPGRTNLGIEQ
AMRVPGSTRTLPGPRWLLQARPGPVGPQRGDIS
LLGEKEIRATRRGLRAFPSRERRSGRPARTA*S
LEGK*ETSRPALPLFPGSLNEPRVLTSCKPKHVE
MIRI*QNNI*VSDSPSPPTPEIGQFTSLTKMIT
WKAAQRQLCGLQISCVGFSVENAPPSRPSHSIP
PS*IDGTLQRPYQREHGLLSGQNDAKILLMSLHG
IVMNICFNIAIISFPKLLLSIWNLINRNEFI*I
*EAYAYL*FLTPYV*RETSGVRYTNIFNCVILN
F*
Frame 2
LPPPPARPWTERRRPGRGDGAAAPAAAAATAAAAATRPACRPFVRPPRPAAPGASGWSVWKVPEGS
GNGLLPLRGVARGPAAPARGVAEAFRHRFHVAG
*LYSVCDGPGTCRPPENLPEAS*SIQPSQALKTQ
RWQREEVQSGGRCCPGQRPPTPPALPGGPVPGP
SLRRARQDPYGAGQTPR*G**PLLREHPDLCGP
HVCADLPSRVPDPAPRGPPAADPRPAPAR*AQP*
GGQWCGQTPSDGGPQCLCCPEAPSSPRGGQPGA
TVPSACTLKNAQAFAGTSRPPEVAFTSGRFRRP
LQHRRWSTDSYPPEGPAEAAG*GEGPEWPGAGRH
G*ADGWPDARRGPWSSSRQPWESGPGRVWRTGG
WPQGHPPWRQLSR*RSAGRR**TLPRGPSSECH
TRGPPAGPRVSTLTWSPPLCKAPRHGEEEVRAP*
VFPVFRRGDCRSGERQEPDVFQRSAGAAAAFGA
RGRCVWGAPKPDAWALEGGAEPSSGCSGGPQRR
PAIGGPAFRGRGAGLHRDRQRPPAPRGRKAAGGG
RRPPPAGAQQCVR*RGGSRTSSDLQSERQCPKR
DH*GCGEGHS*QQRQTGGNLWTENSSNRSRVEK
QTQVPASSGGARRLHQVHRAHPGLPRLHPGRPPG
LWPHLLPPP*LSAQAEGEALGTRGRPRCRCHCR
LPGAVPPAYGHAATRPT*GPAHVTHQQRLIPVQ
RRADPQPLRTQQRLILVRGACAVQHGHLHRPHDP
VLHGGPPEEQEPAGEGVGSAVRLPGGAQQLVRG
PEGGERAQEPLPGRADL*PLPGPAEGGEQPQPL
RLHQR*PHHGSRPEEPRVHRHPGTAARHRG*LLA
DGVGERLRGDRHADTPRGERRPAVLPLLAG*RL
QSLPHL*GEPGLRAHLV*GLPGEELLSEEPADQ
RDAHRDAVPLPELV*PRSPFLLKVPPGLPQKSKQ
VLQGPFLSNNCSLQ*RCRPERHLRPDRHGSQQD
GQRC*RD*YRSDPGALEGPETRHGPDEGAV*VR
ADSRG*GGERHPQGPSPVSGSLRGLRGAPTPRML
SGIMI*L*LCVFYYNCIVIGPLALP*SAQFSS*
KCIFV*SNNNKERFVEKSSYGWRGIGSSIFTCL
KKILFLKAPVHLLG*SCVNNAVASTFEAVSRKSV
LVICHFREGGSTVQEWPGKLAALSEAARHTRHV
WHFSLREFIIGRSRRDRTERPAASGQVRQSQLL
AASPSTLRRVPWTAPSATPVSPSRVIQGLPGGRH
LCTP*VVPT*GWHSPDLTFAKLQMHPNIEMCF*
C*NIDFYVMKTDAPVNAYL*DNHNQEEQIWALS
KL*GSPGAHEPCQAPAGSSRHVPDLWGPREGTFP
SWERRRSGQLGEGCEHFPPGRGDQGDLHALRRA
WKGSEKPADRPCPSSRDHLMNHVF*HHVNLST*
R*FGFDKITFEYPIRHHPLPQK*DNSLH*PR*SH
GRRHRGSCVGCRFPVWGSA*KTHLHPALPTAFL
HLR*MVLSKGPTRGNTAY*ADRMMPKYCLCLYM
VL**ISALI*LSFLFQNYFSLSGI*LIEMNLSEY
RKHMPTCNF*LLMFEEKPPV*DIQIYLIVSY*T
SD
Sequence NM_130843
GCCTCCCGCCGCCTCCCGCGCGGCCATGGACTGAGCGCCGCCGGCCAGGCCGCGGGGATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCCCTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGGCTGCCTGCTCGAGGAGGGCCTCTGCGGAGC
GTCCGAGGCCTGTGTGAACGATGGAGTGTTTGGAAGGTGCCAGAAGGTTCCGGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGC
CTGCGCGTGGCGTTGCAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTATACTCAGTATGTGATGGACCAGGAACTTGCAGACCTCCCGAAAA
CCTACCTGAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGCGTTGGCAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCTGCCCTGGC
CAACGCCCTCCGACGCCACCTGCCCTTCCTGGAGGCCCTGTCCCAGGCCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCCCCC
GCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCTGCGCTGACCTACCCTCCCGGGTCCCGGACCCAGCTCCGCGAGG
ACCTCCTGCCGCGGACCCTCGGCCAGCTCCAGCCAGATGAGCTCAGCCCTAAGGTGGACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGTGC
CTATGCTGCCCAGAGGCCCCCAGCTCCCCCCGGGGAGGGCAGCCTGGAGCCACAGTACCTTCTGCGTGCACCCTCAAGAATGCCCAGGCCTTTGCTGGCA
CCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGACCCCTCCAGCACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGC
AGAGGCAGCCGGCTGAGGTGAGGGGCCTGAGTGGCCTGGAGCTGGACGGCATGGCTGAGCTGATGGCTGGCCTGATGCAAGGCGTGGACCATGGAGTAGC
TCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAACAGGCGGATGGCCCCAAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGAGTG
CAGGACGACGATGATAGACTTTACCAAGAGGTCCATCGTCTGAGTGCCACACTCGGGGGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGCCC
TCCCCTTTGCAAGGCCCCTCGACATGGAGAGGAAGAAGTCCGAGCACCCTGAGTCTTCCCTGTCTTCAGAAGAGGAGACTGCCGGAGTGGAGAACGTCAA
GAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAGCCGCATTCGGAGCCCGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCCTCG
AAGGAGGAGCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGCCTGCAATTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACATCG
TGACAGACAGAGAGGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCCAATGTCCAAAACGTGACCACTGAGGATGTGGAGAAGGCCACAGTTGACAA
CAAAGACAAACTGGAGGAAACCTCTGGACTGAAAATTCTTCAAACCGGAGTCGGGTCGAAAAGCAAACTCAAGTTCCTGCCTCCTCAGGCGGAGCAAGAA
GACTCCACCAAGTTCATCGCGCTCACCCTGGTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCATCTACTGCCTCCGCCATAGCTCTC
AGCACAGGCTGAAGGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCAGATGCCACTGCCGCCTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCG
GCCACCAGACCGACCTGAGGGCCCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGCGACGGGCCGATCCCCAGCCCCTCCGCACGCAGCAGC
GCCTCATCCTGGTCCGAGGAGCCTGTGCAGTCCAACATGGACATCTCCACCGGCCACATGATCCTGTCCTACATGGAGGACCACCTGAAGAACAAGAACC
GGCTGGAGAAGGAGTGGGAAGCGCTGTGCGCCTACCAGGCGGAGCCCAACAGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCT
GGCCGTGCTGACCTATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGACTACATCAACGCTAGCCCCATCATGGATCACGAC
CCGAGGAACCCCGCGTACATCGCCACCCAGGGACCGCTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCTGCGTGGTGATCGTCA
TGCTGACACCCCTCGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGGCCGGATGAAGGCTCCAATCTCTACCACATCTATGAGGTGAACCTGGTCTC
CGAGCACATCTGGTGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAACGAGACGCGCACCGTGACGCAGTTCCACTTCCTGAGT
TGGTATGACCGAGGAGTCCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTTGTCCAATAATTGTTC
ATTGCAGTGACGGTGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATGGTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGAC
CCTGGAGCACTTGAGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGCTGACAGCCGTGGCTGAGGAGGTGAACGCCATCCTC
AAGGCCCTTCCCCAGTGAGCGGCAGCCTCAGGGGCCTCAGGGGAGCCCCCACCCCACGGATGTTGTCAGGAATCATGATCTGACTTTAATTGTGTGTCTT
CTATTATAACTGCATAGTAATAGGGCCCTTAGCTCTCCCGTAGTCAGCGCAGTTTAGCAGTTAAAAGTGTATTTTTGTTTAATCAAACAATAATAAAGAG
AGATTTGTGGAAAAATCCAGTTACGGGTGGAGGGGAATCGGTTCATCAATTTTCACTTGCTTAAAAAAAATACTTTTTCTTAAAGCACCCGTTCACCTTC
TTGGTTGAAGTTGTGTTAACAATGCAGTAGCCAGCACGTTCGAGGCGGTTTCCAGGAAGAGTGTGCTTGTCATCTGCCACTTTCGGGAGGGTGGATCCAC
TGTGCAGGAGTGGCCGGGGAAGCTGGCAGCACTCAGTGAGGCCGCCCGGCACACAAGGCACGTTTGGCATTTCTCTTTGAGAGAGTTTATCATTGGGAGA
AGCCGCAGGGACAGAACTGAACGTCCTGCAGCTTCGGGGCAAGTGAGACAATCACAGCTCCTCGCTGCGTCTCCATCAACACTGCGCCGGGTACCATGGA
CGGCCCCGTCAGCCACACCTGTCAGCCCAAGCAGAGTGATTCAGGGGCTCCCCGGGGGCAGACACCTGTGCACCCCATGAGTAGTGCCCACTTGAGGCTG
GCACTCCCCTGACCTCACCTTTGCAAAGTTACAGATGCACCCCAACATTGAGATGTGTTTTTAATGTTAAAATATTGATTTCTACGTTATGAAAACAGAT
GCCCCCGTGAATGCTTACCTGTGAGATAACCACAACCAGGAAGAACAAATCTGGGCATTGAGCAAGCTATGAGGGTCCCCGGGAGCACACGAACCCTGCC
AGGCCCCCGCTGGCTCCTCCAGGCACGTCCCGGACCTGTGGGGCCCCAGAGAGGGGACATTTCCCTCCTGGGAGAGAAGGAGATCAGGGCAACTCGGAGA
GGGCTGCGAGCATTTCCCTCCCGGGAGAGGAGATCAGGGCGACCTGCACGCACTGCGTAGAGCCTGGAAGGGAAGTGAGAAACCAGCCGACCGGCCCTGC
CCCTCTTCCCGGGATCACTTAATGAACCACGTGTTTTGACATCATGTAAACCTAAGCACGTAGAGATGATTCGGATTTGACAAAATAACATTTGAGTATC
CGATTCGCCATCACCCCCTACCCCAGAAATAGGACAATTCACTTCATTGACCAAGATGATCACATGGAAGGCGGCACAGAGGCAGCTGTGTGGGCTGCAG
ATTTCCTGTGTGGGGTTCAGCGTAGAAAACGCACCTCCATCCCGCCCTTCCCACAGCATTCCTCCATCTTAGATAGATGGTACTCTCCAAAGGCCCTACC
AGAGGGAACACGGCCTACTGAGCGGACAGAATGATGCCAAAATATTGCTTATGTCTCTACATGGTATTGTAATGAATATCTGCTTTAATATAGCTATCAT
TTCTTTTCCAAAATTACTTCTCTCTATCTGGAATTTAATTAATCGAAATGAATTTATCTGAATATAGGAAGCATATGCCTACTTGTAATTTCTAACTCCT
TATGTTTGAAGAGAAACCTCCGGTGTGAGATATACAAATATATTTAATTGTGTCATATTAAACTTCTGATT
- refseq_PTPRN2.F1 (forward primer)
- refseq_PTPRN2.R1 (reverse primer)
- Single exon skipping
Frame 0
ASRRLPRGHGLSAAGQAAGMGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLGCLLEEGLCGA
SEACVNDGVFGRCQKVPAMDFYRYEVSPVALQR
LRVALQKLSGTGFTWQDDYTQYVMDQELADLPK
TYLRRPEASSPARPSKHSVGSERRYSREGGAALA
NALRRHLPFLEALSQAPASDVLARTHTAQDRPP
AEGDDRFSESILTYVAHTSALTYPPGSRTQLRE
DLLPRTLGQLQPDELSPKVDSGVDRHHLMAALSA
YAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLLA
PAAPQKWPSPLGDSEDPSSTGDGARIHTLLKDL
QRQPAEVRGLSGLELDGMAELMAGLMQGVDHGVA
RGSPGRAALGESGEQADGPKATLRGDSFPDDGV
QDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGA
LPFARPLDMERKKSEHPESSLSSEEETAGVENVK
SQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPS
KEEQSLPAGAQEALSDGLQLEVQPSEEEARGYI
VTDREVLGPAVTFKVSANVQNVTTEDVEKATVDN
KDKLEETSGLKILQTGVGSKSKLKFLPPQAEQE
DSTKFIALTLVSLACILGVLLASGLIYCLRHSS
QHRLKEKLSGLGGDPGADATAAYQELCRQRMATR
PPDRPEGPHTSRISSVSSQFSDGPIPSPSARSS
ASSWSEEPVQSNMDISTGHMILSYMEDHLKNKN
RLEKEWEALCAYQAEPNSSFVAQREENVPKNRSL
AVLTYDHSRVLLKAENSHSHSDYINASPIMDHD
PRNPAYIATQGPLPATVADFWQMVWESGCVVIV
MLTPLAENGVRQCYHYWPDEGSNLYHIYEVNLVS
EHIWCEDFLVRSFYLKNLQTNETRTVTQFHFLS
WYDRGVPSSSRSLLDFRRKVNKCYRGRSCPIIV
HCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAAT
LEHLRDQRPGMVQTKEQFEFALTAVAEEVNAIL
KALPQ*AAASGASGEPPPHGCCQES*SDFNCVS
SIITA***GP*LSRSQRSLAVKSVFLFNQTIIKR
DLWKNPVTGGGESVHQFSLA*KKYFFLKHPFTF
LVEVVLTMQ*PARSRRFPGRVCLSSATFGRVDP
LCRSGRGSWQHSVRPPGTQGTFGISL*ESLSLGE
AAGTELNVLQLRGK*DNHSSSLRLHQHCAGYHG
RPRQPHLSAQAE*FRGSPGADTCAPHE*CPLEA
GTPLTSPLQSYRCTPTLRCVFNVKILISTL*KQM
PP*MLTCEITTTRKNKSGH*ASYEGPREHTNPA
RPPLAPPGTSRTCGAPERGHFPPGREGDQGNSE
RAASISLPGEEIRATCTHCVEPGREVRNQPTGPA
PLPGIT**TTCFDIM*T*ARRDDSDLTK*HLSI
RFAITPYPRNRTIHFIDQDDHMEGGTEAAVWAA
DFLCGVQRRKRTSIPPFPQHSSILDRWYSPKALP
EGTRPTERTE*CQNIAYVSTWYCNEYLL*YSYH
FFSKITSLYLEFN*SK*IYLNIGSICLLVISNS
LCLKRNLRCEIYKYI*LCHIKLLI
Frame 1
PPAASRAAMD*APPARPRGWGRRSRCCCCYCCCCRHASCLPPLRPSPAAGSSRGVWAACSRRASAE
RPRPV*TMECLEGARRFRQWTFTATRCRPWPCSA
CAWRCRSFPAQVSRGRMTILSM*WTRNLQTSRK
PT*GVLKHPAQPGPQNTALAARGGTVGRAVLPW
PTPSDATCPSWRPCPRPQPQTCSPGPIRRRTDPP
LRVMTASPRAS*PMWPTRLR*PTLPGPGPSSAR
TSCRGPSASSSQMSSALRWTVVWTDTI*WRPSV
PMLPRGPQLPPGRAAWSHSTFCVHPQECPGLCWH
QPPPRSGLHLWEIPKTPPAQAMEHGFIPS*RTC
RGSRLR*GA*VAWSWTAWLS*WLA*CKAWTME*
LEAALGERPWESLENRRMAPRPPSVETAFQMTEC
RTTMIDFTKRSIV*VPHSGASCRTTGLDSYLEP
SPLQGPSTWRGRSPSTLSLPCLQKRRLPEWRTS
RARRIPKICWGSSRIRSPGPLRLGSSKTRCLGPR
RRSRAFQRVLRRPSATACNWRSSLPRKRRGATS
*QTERFSDQQ*PSK*APMSKT*PLRMWRRPQLT
TKTNWRKPLD*KFFKPESGRKANSSSCLLRRSKK
TPPSSSRSPWSPSPASWASSWPLASSTASAIAL
STG*RRSSRD*GATQVQMPLPPTRSCAASVWPR
GHQTDLRARTRHASAASHPSSATGRSPAPPHAAA
PHPGPRSLCSPTWTSPPAT*SCPTWRTT*RTRT
GWRRSGKRCAPTRRSPTARSWPRGRRTCPRTAP
WPC*PMTTPGSC*RRRTATATQTTSTLAPSWITT
RGTPRTSPPRDRCPPPWLTFGRWCGRAAAW*SS
C*HPSRRTASGSATTTGRMKAPISTTSMR*TWS
PSTSGVRTSW*GASI*RTCRPTRRAP*RSSTS*V
GMTEESLPPQGPSWTSAEK*TSATGAVLVQ*LF
IAVTVQAGAAPTS*STWFSTRWPKVLKRLISQR
PWST*GTRDPAWSRRRSSLSSR*QPWLRR*TPSS
RPFPSERQPQGPQGSPHPTDVVRNHDLTLIVCL
LL*LHSNRALSSPVVSAV*QLKVYFCLIKQ**R
EICGKIQLRVEGNRFINFHLLKKNTFS*STRSPS
WLKLC*QCSSQHVRGGFQEECACHLPLSGGWIH
CAGVAGEAGSTQ*GRPAHKARLAFLFERVYHWE
KPQGQN*TSCSFGASETITAPRCVSINTAPGTMD
GPVSHTCQPKQSDSGAPRGQTPVHPMSSAHLRL
ALP*PHLCKVTDAPQH*DVFLMLKY*FLRYENR
CPRECLPVR*PQPGRTNLGIEQAMRVPGSTRTLP
GPRWLLQARPGPVGPQRGDISLLGEKEIRATRR
GLRAFPSRERRSGRPARTA*SLEGK*ETSRPAL
PLFPGSLNEPRVLTSCKPKHVEMIRI*QNNI*VS
DSPSPPTPEIGQFTSLTKMITWKAAQRQLCGLQ
ISCVGFSVENAPPSRPSHSIPPS*IDGTLQRPY
QREHGLLSGQNDAKILLMSLHGIVMNICFNIAII
SFPKLLLSIWNLINRNEFI*I*EAYAYL*FLTP
YV*RETSGVRYTNIFNCVILNF*
Frame 2
LPPPPARPWTERRRPGRGDGAAAPAAAAATAAAAATRPACRPFVRPPRPAAPGASGLPARGGPLRS
VRGLCERWSVWKVPEGSGNGLLPLRGVARGPAA
PARGVAEAFRHRFHVAG*LYSVCDGPGTCRPPEN
LPEAS*SIQPSQALKTQRWQREEVQSGGRCCPG
QRPPTPPALPGGPVPGPSLRRARQDPYGAGQTP
R*G**PLLREHPDLCGPHVCADLPSRVPDPAPRG
PPAADPRPAPAR*AQP*GGQWCGQTPSDGGPQC
LCCPEAPSSPRGGQPGATVPSACTLKNAQAFAG
TSRPPEVAFTSGRFRRPLQHRRWSTDSYPPEGPA
EAAG*GEGPEWPGAGRHG*ADGWPDARRGPWSS
SRQPWESGPGRVWRTGGWPQGHPPWRQLSR*RS
AGRR**TLPRGPSSECHTRGPPAGPRVSTLTWSP
PLCKAPRHGEEEVRAP*VFPVFRRGDCRSGERQ
EPDVFQRSAGAAAAFGARGRCVWGAPKPDAWAL
EGGAEPSSGCSGGPQRRPAIGGPAFRGRGAGLHR
DRQRGSRTSSDLQSERQCPKRDH*GCGEGHS*Q
QRQTGGNLWTENSSNRSRVEKQTQVPASSGGAR
RLHQVHRAHPGLPRLHPGRPPGLWPHLLPPP*LS
AQAEGEALGTRGRPRCRCHCRLPGAVPPAYGHA
ATRPT*GPAHVTHQQRLIPVQRRADPQPLRTQQ
RLILVRGACAVQHGHLHRPHDPVLHGGPPEEQEP
AGEGVGSAVRLPGGAQQLVRGPEGGERAQEPLP
GRADL*PLPGPAEGGEQPQPLRLHQR*PHHGSR
PEEPRVHRHPGTAARHRG*LLADGVGERLRGDRH
ADTPRGERRPAVLPLLAG*RLQSLPHL*GEPGL
RAHLV*GLPGEELLSEEPADQRDAHRDAVPLPE
LV*PRSPFLLKVPPGLPQKSKQVLQGPFLSNNCS
LQ*RCRPERHLRPDRHGSQQDGQRC*RD*YRSD
PGALEGPETRHGPDEGAV*VRADSRG*GGERHP
QGPSPVSGSLRGLRGAPTPRMLSGIMI*L*LCVF
YYNCIVIGPLALP*SAQFSS*KCIFV*SNNNKE
RFVEKSSYGWRGIGSSIFTCLKKILFLKAPVHL
LG*SCVNNAVASTFEAVSRKSVLVICHFREGGST
VQEWPGKLAALSEAARHTRHVWHFSLREFIIGR
SRRDRTERPAASGQVRQSQLLAASPSTLRRVPW
TAPSATPVSPSRVIQGLPGGRHLCTP*VVPT*GW
HSPDLTFAKLQMHPNIEMCF*C*NIDFYVMKTD
APVNAYL*DNHNQEEQIWALSKL*GSPGAHEPC
QAPAGSSRHVPDLWGPREGTFPSWERRRSGQLGE
GCEHFPPGRGDQGDLHALRRAWKGSEKPADRPC
PSSRDHLMNHVF*HHVNLST*R*FGFDKITFEY
PIRHHPLPQK*DNSLH*PR*SHGRRHRGSCVGCR
FPVWGSA*KTHLHPALPTAFLHLR*MVLSKGPT
RGNTAY*ADRMMPKYCLCLYMVL**ISALI*LS
FLFQNYFSLSGI*LIEMNLSEYRKHMPTCNF*LL
MFEEKPPV*DIQIYLIVSY*TSD
Sequence NM_002847
GCCTCCCGCCGCCTCCCGCGCGGCCATGGACTGAGCGCCGCCGGCCAGGCCGCGGGGATGGGGCCGCCGCTCCCGCTGCTGCTGCTGCTACTGCTGCTGCTGCCGCCACGCGTCCTGCCTGCCGCCCCTTCGTCCGTCCCCCGCGGCCGGCAGCTCCCGGGGCGTCTGGGCTGCCTGCTCGAGGAGGGCCTCTGCGGAGC
GTCCGAGGCCTGTGTGAACGATGGAGTGTTTGGAAGGTGCCAGAAGGTTCCGGCAATGGACTTTTACCGCTACGAGGTGTCGCCCGTGGCCCTGCAGCGC
CTGCGCGTGGCGTTGCAGAAGCTTTCCGGCACAGGTTTCACGTGGCAGGATGACTATACTCAGTATGTGATGGACCAGGAACTTGCAGACCTCCCGAAAA
CCTACCTGAGGCGTCCTGAAGCATCCAGCCCAGCCAGGCCCTCAAAACACAGCGTTGGCAGCGAGAGGAGGTACAGTCGGGAGGGCGGTGCTGCCCTGGC
CAACGCCCTCCGACGCCACCTGCCCTTCCTGGAGGCCCTGTCCCAGGCCCCAGCCTCAGACGTGCTCGCCAGGACCCATACGGCGCAGGACAGACCCCCC
GCTGAGGGTGATGACCGCTTCTCCGAGAGCATCCTGACCTATGTGGCCCACACGTCTGCGCTGACCTACCCTCCCGGGTCCCGGACCCAGCTCCGCGAGG
ACCTCCTGCCGCGGACCCTCGGCCAGCTCCAGCCAGATGAGCTCAGCCCTAAGGTGGACAGTGGTGTGGACAGACACCATCTGATGGCGGCCCTCAGTGC
CTATGCTGCCCAGAGGCCCCCAGCTCCCCCCGGGGAGGGCAGCCTGGAGCCACAGTACCTTCTGCGTGCACCCTCAAGAATGCCCAGGCCTTTGCTGGCA
CCAGCCGCCCCCCAGAAGTGGCCTTCACCTCTGGGAGATTCCGAAGACCCCTCCAGCACAGGCGATGGAGCACGGATTCATACCCTCCTGAAGGACCTGC
AGAGGCAGCCGGCTGAGGTGAGGGGCCTGAGTGGCCTGGAGCTGGACGGCATGGCTGAGCTGATGGCTGGCCTGATGCAAGGCGTGGACCATGGAGTAGC
TCGAGGCAGCCCTGGGAGAGCGGCCCTGGGAGAGTCTGGAGAACAGGCGGATGGCCCCAAGGCCACCCTCCGTGGAGACAGCTTTCCAGATGACGGAGTG
CAGGACGACGATGATAGACTTTACCAAGAGGTCCATCGTCTGAGTGCCACACTCGGGGGCCTCCTGCAGGACCACGGGTCTCGACTCTTACCTGGAGCCC
TCCCCTTTGCAAGGCCCCTCGACATGGAGAGGAAGAAGTCCGAGCACCCTGAGTCTTCCCTGTCTTCAGAAGAGGAGACTGCCGGAGTGGAGAACGTCAA
GAGCCAGACGTATTCCAAAGATCTGCTGGGGCAGCAGCCGCATTCGGAGCCCGGGGCCGCTGCGTTTGGGGAGCTCCAAAACCAGATGCCTGGGCCCTCG
AAGGAGGAGCAGAGCCTTCCAGCGGGTGCTCAGGAGGCCCTCAGCGACGGCCTGCAATTGGAGGTCCAGCCTTCCGAGGAAGAGGCGCGGGGCTACATCG
TGACAGACAGAGACCCCCTGCGCCCCGAGGAAGGAAGGCGGCTGGTGGAGGACGTCGCCCGCCTCCTGCAGGTGCCCAGCAGTGCGTTCGCTGACGTGGA
GGTTCTCGGACCAGCAGTGACCTTCAAAGTGAGCGCCAATGTCCAAAACGTGACCACTGAGGATGTGGAGAAGGCCACAGTTGACAACAAAGACAAACTG
GAGGAAACCTCTGGACTGAAAATTCTTCAAACCGGAGTCGGGTCGAAAAGCAAACTCAAGTTCCTGCCTCCTCAGGCGGAGCAAGAAGACTCCACCAAGT
TCATCGCGCTCACCCTGGTCTCCCTCGCCTGCATCCTGGGCGTCCTCCTGGCCTCTGGCCTCATCTACTGCCTCCGCCATAGCTCTCAGCACAGGCTGAA
GGAGAAGCTCTCGGGACTAGGGGGCGACCCAGGTGCAGATGCCACTGCCGCCTACCAGGAGCTGTGCCGCCAGCGTATGGCCACGCGGCCACCAGACCGA
CCTGAGGGCCCGCACACGTCACGCATCAGCAGCGTCTCATCCCAGTTCAGCGACGGGCCGATCCCCAGCCCCTCCGCACGCAGCAGCGCCTCATCCTGGT
CCGAGGAGCCTGTGCAGTCCAACATGGACATCTCCACCGGCCACATGATCCTGTCCTACATGGAGGACCACCTGAAGAACAAGAACCGGCTGGAGAAGGA
GTGGGAAGCGCTGTGCGCCTACCAGGCGGAGCCCAACAGCTCGTTCGTGGCCCAGAGGGAGGAGAACGTGCCCAAGAACCGCTCCCTGGCCGTGCTGACC
TATGACCACTCCCGGGTCCTGCTGAAGGCGGAGAACAGCCACAGCCACTCAGACTACATCAACGCTAGCCCCATCATGGATCACGACCCGAGGAACCCCG
CGTACATCGCCACCCAGGGACCGCTGCCCGCCACCGTGGCTGACTTTTGGCAGATGGTGTGGGAGAGCGGCTGCGTGGTGATCGTCATGCTGACACCCCT
CGCGGAGAACGGCGTCCGGCAGTGCTACCACTACTGGCCGGATGAAGGCTCCAATCTCTACCACATCTATGAGGTGAACCTGGTCTCCGAGCACATCTGG
TGTGAGGACTTCCTGGTGAGGAGCTTCTATCTGAAGAACCTGCAGACCAACGAGACGCGCACCGTGACGCAGTTCCACTTCCTGAGTTGGTATGACCGAG
GAGTCCCTTCCTCCTCAAGGTCCCTCCTGGACTTCCGCAGAAAAGTAAACAAGTGCTACAGGGGCCGTTCTTGTCCAATAATTGTTCATTGCAGTGACGG
TGCAGGCCGGAGCGGCACCTACGTCCTGATCGACATGGTTCTCAACAAGATGGCCAAAGGTGCTAAAGAGATTGATATCGCAGCGACCCTGGAGCACTTG
AGGGACCAGAGACCCGGCATGGTCCAGACGAAGGAGCAGTTTGAGTTCGCGCTGACAGCCGTGGCTGAGGAGGTGAACGCCATCCTCAAGGCCCTTCCCC
AGTGAGCGGCAGCCTCAGGGGCCTCAGGGGAGCCCCCACCCCACGGATGTTGTCAGGAATCATGATCTGACTTTAATTGTGTGTCTTCTATTATAACTGC
ATAGTAATAGGGCCCTTAGCTCTCCCGTAGTCAGCGCAGTTTAGCAGTTAAAAGTGTATTTTTGTTTAATCAAACAATAATAAAGAGAGATTTGTGGAAA
AATCCAGTTACGGGTGGAGGGGAATCGGTTCATCAATTTTCACTTGCTTAAAAAAAATACTTTTTCTTAAAGCACCCGTTCACCTTCTTGGTTGAAGTTG
TGTTAACAATGCAGTAGCCAGCACGTTCGAGGCGGTTTCCAGGAAGAGTGTGCTTGTCATCTGCCACTTTCGGGAGGGTGGATCCACTGTGCAGGAGTGG
CCGGGGAAGCTGGCAGCACTCAGTGAGGCCGCCCGGCACACAAGGCACGTTTGGCATTTCTCTTTGAGAGAGTTTATCATTGGGAGAAGCCGCAGGGACA
GAACTGAACGTCCTGCAGCTTCGGGGCAAGTGAGACAATCACAGCTCCTCGCTGCGTCTCCATCAACACTGCGCCGGGTACCATGGACGGCCCCGTCAGC
CACACCTGTCAGCCCAAGCAGAGTGATTCAGGGGCTCCCCGGGGGCAGACACCTGTGCACCCCATGAGTAGTGCCCACTTGAGGCTGGCACTCCCCTGAC
CTCACCTTTGCAAAGTTACAGATGCACCCCAACATTGAGATGTGTTTTTAATGTTAAAATATTGATTTCTACGTTATGAAAACAGATGCCCCCGTGAATG
CTTACCTGTGAGATAACCACAACCAGGAAGAACAAATCTGGGCATTGAGCAAGCTATGAGGGTCCCCGGGAGCACACGAACCCTGCCAGGCCCCCGCTGG
CTCCTCCAGGCACGTCCCGGACCTGTGGGGCCCCAGAGAGGGGACATTTCCCTCCTGGGAGAGAAGGAGATCAGGGCAACTCGGAGAGGGCTGCGAGCAT
TTCCCTCCCGGGAGAGGAGATCAGGGCGACCTGCACGCACTGCGTAGAGCCTGGAAGGGAAGTGAGAAACCAGCCGACCGGCCCTGCCCCTCTTCCCGGG
ATCACTTAATGAACCACGTGTTTTGACATCATGTAAACCTAAGCACGTAGAGATGATTCGGATTTGACAAAATAACATTTGAGTATCCGATTCGCCATCA
CCCCCTACCCCAGAAATAGGACAATTCACTTCATTGACCAAGATGATCACATGGAAGGCGGCACAGAGGCAGCTGTGTGGGCTGCAGATTTCCTGTGTGG
GGTTCAGCGTAGAAAACGCACCTCCATCCCGCCCTTCCCACAGCATTCCTCCATCTTAGATAGATGGTACTCTCCAAAGGCCCTACCAGAGGGAACACGG
CCTACTGAGCGGACAGAATGATGCCAAAATATTGCTTATGTCTCTACATGGTATTGTAATGAATATCTGCTTTAATATAGCTATCATTTCTTTTCCAAAA
TTACTTCTCTCTATCTGGAATTTAATTAATCGAAATGAATTTATCTGAATATAGGAAGCATATGCCTACTTGTAATTTCTAACTCCTTATGTTTGAAGAG
AAACCTCCGGTGTGAGATATACAAATATATTTAATTGTGTCATATTAAACTTCTGATT
- refseq_PTPRN2.F1 (forward primer)
- refseq_PTPRN2.R1 (reverse primer)
- Single exon skipping
Frame 0
ASRRLPRGHGLSAAGQAAGMGPPLPLLLLLLLLLPPRVLPAAPSSVPRGRQLPGRLGCLLEEGLCGA
SEACVNDGVFGRCQKVPAMDFYRYEVSPVALQR
LRVALQKLSGTGFTWQDDYTQYVMDQELADLPK
TYLRRPEASSPARPSKHSVGSERRYSREGGAALA
NALRRHLPFLEALSQAPASDVLARTHTAQDRPP
AEGDDRFSESILTYVAHTSALTYPPGSRTQLRE
DLLPRTLGQLQPDELSPKVDSGVDRHHLMAALSA
YAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLLA
PAAPQKWPSPLGDSEDPSSTGDGARIHTLLKDL
QRQPAEVRGLSGLELDGMAELMAGLMQGVDHGVA
RGSPGRAALGESGEQADGPKATLRGDSFPDDGV
QDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGA
LPFARPLDMERKKSEHPESSLSSEEETAGVENVK
SQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPS
KEEQSLPAGAQEALSDGLQLEVQPSEEEARGYI
VTDRDPLRPEEGRRLVEDVARLLQVPSSAFADVE
VLGPAVTFKVSANVQNVTTEDVEKATVDNKDKL
EETSGLKILQTGVGSKSKLKFLPPQAEQEDSTK
FIALTLVSLACILGVLLASGLIYCLRHSSQHRLK
EKLSGLGGDPGADATAAYQELCRQRMATRPPDR
PEGPHTSRISSVSSQFSDGPIPSPSARSSASSW
SEEPVQSNMDISTGHMILSYMEDHLKNKNRLEKE
WEALCAYQAEPNSSFVAQREENVPKNRSLAVLT
YDHSRVLLKAENSHSHSDYINASPIMDHDPRNP
AYIATQGPLPATVADFWQMVWESGCVVIVMLTPL
AENGVRQCYHYWPDEGSNLYHIYEVNLVSEHIW
CEDFLVRSFYLKNLQTNETRTVTQFHFLSWYDR
GVPSSSRSLLDFRRKVNKCYRGRSCPIIVHCSDG
AGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHL
RDQRPGMVQTKEQFEFALTAVAEEVNAILKALP
Q*AAASGASGEPPPHGCCQES*SDFNCVSSIITA
***GP*LSRSQRSLAVKSVFLFNQTIIKRDLWK
NPVTGGGESVHQFSLA*KKYFFLKHPFTFLVEV
VLTMQ*PARSRRFPGRVCLSSATFGRVDPLCRSG
RGSWQHSVRPPGTQGTFGISL*ESLSLGEAAGT
ELNVLQLRGK*DNHSSSLRLHQHCAGYHGRPRQ
PHLSAQAE*FRGSPGADTCAPHE*CPLEAGTPLT
SPLQSYRCTPTLRCVFNVKILISTL*KQMPP*M
LTCEITTTRKNKSGH*ASYEGPREHTNPARPPL
APPGTSRTCGAPERGHFPPGREGDQGNSERAASI
SLPGEEIRATCTHCVEPGREVRNQPTGPAPLPG
IT**TTCFDIM*T*ARRDDSDLTK*HLSIRFAI
TPYPRNRTIHFIDQDDHMEGGTEAAVWAADFLCG
VQRRKRTSIPPFPQHSSILDRWYSPKALPEGTR
PTERTE*CQNIAYVSTWYCNEYLL*YSYHFFSK
ITSLYLEFN*SK*IYLNIGSICLLVISNSLCLKR
NLRCEIYKYI*LCHIKLLI
Frame 1
PPAASRAAMD*APPARPRGWGRRSRCCCCYCCCCRHASCLPPLRPSPAAGSSRGVWAACSRRASAE
RPRPV*TMECLEGARRFRQWTFTATRCRPWPCSA
CAWRCRSFPAQVSRGRMTILSM*WTRNLQTSRK
PT*GVLKHPAQPGPQNTALAARGGTVGRAVLPW
PTPSDATCPSWRPCPRPQPQTCSPGPIRRRTDPP
LRVMTASPRAS*PMWPTRLR*PTLPGPGPSSAR
TSCRGPSASSSQMSSALRWTVVWTDTI*WRPSV
PMLPRGPQLPPGRAAWSHSTFCVHPQECPGLCWH
QPPPRSGLHLWEIPKTPPAQAMEHGFIPS*RTC
RGSRLR*GA*VAWSWTAWLS*WLA*CKAWTME*
LEAALGERPWESLENRRMAPRPPSVETAFQMTEC
RTTMIDFTKRSIV*VPHSGASCRTTGLDSYLEP
SPLQGPSTWRGRSPSTLSLPCLQKRRLPEWRTS
RARRIPKICWGSSRIRSPGPLRLGSSKTRCLGPR
RRSRAFQRVLRRPSATACNWRSSLPRKRRGATS
*QTETPCAPRKEGGWWRTSPASCRCPAVRSLTW
RFSDQQ*PSK*APMSKT*PLRMWRRPQLTTKTNW
RKPLD*KFFKPESGRKANSSSCLLRRSKKTPPS
SSRSPWSPSPASWASSWPLASSTASAIALSTG*
RRSSRD*GATQVQMPLPPTRSCAASVWPRGHQTD
LRARTRHASAASHPSSATGRSPAPPHAAAPHPG
PRSLCSPTWTSPPAT*SCPTWRTT*RTRTGWRR
SGKRCAPTRRSPTARSWPRGRRTCPRTAPWPC*P
MTTPGSC*RRRTATATQTTSTLAPSWITTRGTP
RTSPPRDRCPPPWLTFGRWCGRAAAW*SSC*HP
SRRTASGSATTTGRMKAPISTTSMR*TWSPSTSG
VRTSW*GASI*RTCRPTRRAP*RSSTS*VGMTE
ESLPPQGPSWTSAEK*TSATGAVLVQ*LFIAVT
VQAGAAPTS*STWFSTRWPKVLKRLISQRPWST*
GTRDPAWSRRRSSLSSR*QPWLRR*TPSSRPFP
SERQPQGPQGSPHPTDVVRNHDLTLIVCLLL*L
HSNRALSSPVVSAV*QLKVYFCLIKQ**REICGK
IQLRVEGNRFINFHLLKKNTFS*STRSPSWLKL
C*QCSSQHVRGGFQEECACHLPLSGGWIHCAGV
AGEAGSTQ*GRPAHKARLAFLFERVYHWEKPQGQ
N*TSCSFGASETITAPRCVSINTAPGTMDGPVS
HTCQPKQSDSGAPRGQTPVHPMSSAHLRLALP*
PHLCKVTDAPQH*DVFLMLKY*FLRYENRCPREC
LPVR*PQPGRTNLGIEQAMRVPGSTRTLPGPRW
LLQARPGPVGPQRGDISLLGEKEIRATRRGLRA
FPSRERRSGRPARTA*SLEGK*ETSRPALPLFPG
SLNEPRVLTSCKPKHVEMIRI*QNNI*VSDSPS
PPTPEIGQFTSLTKMITWKAAQRQLCGLQISCV
GFSVENAPPSRPSHSIPPS*IDGTLQRPYQREHG
LLSGQNDAKILLMSLHGIVMNICFNIAIISFPK
LLLSIWNLINRNEFI*I*EAYAYL*FLTPYV*R
ETSGVRYTNIFNCVILNF*
Frame 2
LPPPPARPWTERRRPGRGDGAAAPAAAAATAAAAATRPACRPFVRPPRPAAPGASGLPARGGPLRS
VRGLCERWSVWKVPEGSGNGLLPLRGVARGPAA
PARGVAEAFRHRFHVAG*LYSVCDGPGTCRPPEN
LPEAS*SIQPSQALKTQRWQREEVQSGGRCCPG
QRPPTPPALPGGPVPGPSLRRARQDPYGAGQTP
R*G**PLLREHPDLCGPHVCADLPSRVPDPAPRG
PPAADPRPAPAR*AQP*GGQWCGQTPSDGGPQC
LCCPEAPSSPRGGQPGATVPSACTLKNAQAFAG
TSRPPEVAFTSGRFRRPLQHRRWSTDSYPPEGPA
EAAG*GEGPEWPGAGRHG*ADGWPDARRGPWSS
SRQPWESGPGRVWRTGGWPQGHPPWRQLSR*RS
AGRR**TLPRGPSSECHTRGPPAGPRVSTLTWSP
PLCKAPRHGEEEVRAP*VFPVFRRGDCRSGERQ
EPDVFQRSAGAAAAFGARGRCVWGAPKPDAWAL
EGGAEPSSGCSGGPQRRPAIGGPAFRGRGAGLHR
DRQRPPAPRGRKAAGGGRRPPPAGAQQCVR*RG
GSRTSSDLQSERQCPKRDH*GCGEGHS*QQRQT
GGNLWTENSSNRSRVEKQTQVPASSGGARRLHQV
HRAHPGLPRLHPGRPPGLWPHLLPPP*LSAQAE
GEALGTRGRPRCRCHCRLPGAVPPAYGHAATRP
T*GPAHVTHQQRLIPVQRRADPQPLRTQQRLILV
RGACAVQHGHLHRPHDPVLHGGPPEEQEPAGEG
VGSAVRLPGGAQQLVRGPEGGERAQEPLPGRAD
L*PLPGPAEGGEQPQPLRLHQR*PHHGSRPEEPR
VHRHPGTAARHRG*LLADGVGERLRGDRHADTP
RGERRPAVLPLLAG*RLQSLPHL*GEPGLRAHL
V*GLPGEELLSEEPADQRDAHRDAVPLPELV*PR
SPFLLKVPPGLPQKSKQVLQGPFLSNNCSLQ*R
CRPERHLRPDRHGSQQDGQRC*RD*YRSDPGAL
EGPETRHGPDEGAV*VRADSRG*GGERHPQGPSP
VSGSLRGLRGAPTPRMLSGIMI*L*LCVFYYNC
IVIGPLALP*SAQFSS*KCIFV*SNNNKERFVE
KSSYGWRGIGSSIFTCLKKILFLKAPVHLLG*SC
VNNAVASTFEAVSRKSVLVICHFREGGSTVQEW
PGKLAALSEAARHTRHVWHFSLREFIIGRSRRD
RTERPAASGQVRQSQLLAASPSTLRRVPWTAPSA
TPVSPSRVIQGLPGGRHLCTP*VVPT*GWHSPD
LTFAKLQMHPNIEMCF*C*NIDFYVMKTDAPVN
AYL*DNHNQEEQIWALSKL*GSPGAHEPCQAPAG
SSRHVPDLWGPREGTFPSWERRRSGQLGEGCEH
FPPGRGDQGDLHALRRAWKGSEKPADRPCPSSR
DHLMNHVF*HHVNLST*R*FGFDKITFEYPIRHH
PLPQK*DNSLH*PR*SHGRRHRGSCVGCRFPVW
GSA*KTHLHPALPTAFLHLR*MVLSKGPTRGNT
AY*ADRMMPKYCLCLYMVL**ISALI*LSFLFQN
YFSLSGI*LIEMNLSEYRKHMPTCNF*LLMFEE
KPPV*DIQIYLIVSY*TSD
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