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Sequences

Sequence NM_080922

AGAGGAGGAAATTGTTCCTCGTCTGATAAGACAACAGTGGAGAAAGGACGCATGCTGTTTCTTAGGGACACGGCTGACTTCCAGATATGACCATGTATTT GTGGCTTAAACTCTTGGCATTTGGCTTTGCCTTTCTGGACACAGAAGTATTTGTGACAGGGCAAAGCCCAACACCTTCCCCCACTGGATTGACTACAGCA AAGATGCCCAGTGTTCCACTTTCAAGTGACCCCTTACCTACTCACACCACTGCATTCTCACCCGCAAGCACCTTTGAAAGAGAAAATGACTTCTCAGAGA CCACAACTTCTCTTAGTCCAGACAATACTTCCACCCAAGTATCCCCGGACTCTTTGGATAATGCTAGTGCTTTTAATACCACAGGTGTTTCATCAGTACA GACGCCTCACCTTCCCACGCACGCAGACTCGCAGACGCCCTCTGCTGGAACTGACACGCAGACATTCAGCGGCTCCGCCGCCAATGCAAAACTCAACCCT ACCCCAGGCAGCAATGCTATCTCAGATGCCTACCTTAATGCCTCTGAAACAACCACTCTGAGCCCTTCTGGAAGCGCTGTCATTTCAACCACAACAATAG CTACTACTCCATCTAAGCCAACATGTGATGAAAAATATGCAAACATCACTGTGGATTACTTATATAACAAGGAAACTAAATTATTTACAGCAAAGCTAAA TGTTAATGAGAATGTGGAATGTGGAAACAATACTTGCACAAACAATGAGGTGCATAACCTTACAGAATGTAAAAATGCGTCTGTTTCCATATCTCATAAT TCATGTACTGCTCCTGATAAGACATTAATATTAGATGTGCCACCAGGGGTTGAAAAGTTTCAGTTACATGATTGTACACAAGTTGAAAAAGCAGATACTA CTATTTGTTTAAAATGGAAAAATATTGAAACCTTTACTTGTGATACACAGAATATTACCTACAGATTTCAGTGTGGTAATATGATATTTGATAATAAAGA AATTAAATTAGAAAACCTTGAACCCGAACATGAGTATAAGTGTGACTCAGAAATACTCTATAATAACCACAAGTTTACTAACGCAAGTAAAATTATTAAA ACAGATTTTGGGAGTCCAGGAGAGCCTCAGATTATTTTTTGTAGAAGTGAAGCTGCACATCAAGGAGTAATTACCTGGAATCCCCCTCAAAGATCATTTC ATAATTTTACCCTCTGTTATATAAAAGAGACAGAAAAAGATTGCCTCAATCTGGATAAAAACCTGATCAAATATGATTTGCAAAATTTAAAACCTTATAC GAAATATGTTTTATCATTACATGCCTACATCATTGCAAAAGTGCAACGTAATGGAAGTGCTGCAATGTGTCATTTCACAACTAAAAGTGCTCCTCCAAGC CAGGTCTGGAACATGACTGTCTCCATGACATCAGATAATAGTATGCATGTCAAGTGTAGGCCTCCCAGGGACCGTAATGGCCCCCATGAACGTTACCATT TGGAAGTTGAAGCTGGAAATACTCTGGTTAGAAATGAGTCGCATAAGAATTGCGATTTCCGTGTAAAAGATCTTCAATATTCAACAGACTACACTTTTAA GGCCTATTTTCACAATGGAGACTATCCTGGAGAACCCTTTATTTTACATCATTCAACATCTTATAATTCTAAGGCACTGATAGCATTTCTGGCATTTCTG ATTATTGTGACATCAATAGCCCTGCTTGTTGTTCTCTACAAAATCTATGATCTACATAAGAAAAGATCCTGCAATTTAGATGAACAGCAGGAGCTTGTTG AAAGGGATGATGAAAAACAACTGATGAATGTGGAGCCAATCCATGCAGATATTTTGTTGGAAACTTATAAGAGGAAGATTGCTGATGAAGGAAGACTTTT TCTGGCTGAATTTCAGAGCATCCCGCGGGTGTTCAGCAAGTTTCCTATAAAGGAAGCTCGAAAGCCCTTTAACCAGAATAAAAACCGTTATGTTGACATT CTTCCTTATGATTATAACCGTGTTGAACTCTCTGAGATAAACGGAGATGCAGGGTCAAACTACATAAATGCCAGCTATATTGATGGTTTCAAAGAACCCA GGAAATACATTGCTGCACAAGGTCCCAGGGATGAAACTGTTGATGATTTCTGGAGGATGATTTGGGAACAGAAAGCCACAGTTATTGTCATGGTCACTCG ATGTGAAGAAGGAAACAGGAACAAGTGTGCAGAATACTGGCCGTCAATGGAAGAGGGCACTCGGGCTTTTGGAGATGTTGTTGTAAAGATCAACCAGCAC AAAAGATGTCCAGATTACATCATTCAGAAATTGAACATTGTAAATAAAAAAGAAAAAGCAACTGGAAGAGAGGTGACTCACATTCAGTTCACCAGCTGGC CAGACCACGGGGTGCCTGAGGATCCTCACTTGCTCCTCAAACTGAGAAGGAGAGTGAATGCCTTCAGCAATTTCTTCAGTGGTCCCATTGTGGTGCACTG CAGTGCTGGTGTTGGGCGCACAGGAACCTATATCGGAATTGATGCCATGCTAGAAGGCCTGGAAGCCGAGAACAAAGTGGATGTTTATGGTTATGTTGTC AAGCTAAGGCGACAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATCCATCAGGCTTTGGTGGAATACAATCAGTTTGGAGAAACAGAAG TGAATTTGTCTGAATTACATCCATATCTACATAACATGAAGAAAAGGGATCCACCCAGTGAGCCGTCTCCACTAGAGGCTGAATTCCAGAGACTTCCTTC ATATAGGAGCTGGAGGACACAGCACATTGGAAATCAAGAAGAAAATAAAAGTAAAAACAGGAATTCTAATGTCATCCCATATGACTATAACAGAGTGCCA CTTAAACATGAGCTGGAAATGAGTAAAGAGAGTGAGCATGATTCAGATGAATCCTCTGATGATGACAGTGATTCAGAGGAACCAAGCAAATACATCAATG CATCTTTTATAATGAGCTACTGGAAACCTGAAGTGATGATTGCTGCTCAGGGACCACTGAAGGAGACCATTGGTGACTTTTGGCAGATGATCTTCCAAAG AAAAGTCAAAGTTATTGTTATGCTGACAGAACTGAAACATGGAGACCAGGAAATCTGTGCTCAGTACTGGGGAGAAGGAAAGCAAACATATGGAGATATT GAAGTTGACCTGAAAGACACAGACAAATCTTCAACTTATACCCTTCGTGTCTTTGAACTGAGACATTCCAAGAGGAAAGACTCTCGAACTGTGTACCAGT ACCAATATACAAACTGGAGTGTGGAGCAGCTTCCTGCAGAACCCAAGGAATTAATCTCTATGATTCAGGTCGTCAAACAAAAACTTCCCCAGAAGAATTC CTCTGAAGGGAACAAGCATCACAAGAGTACACCTCTACTCATTCACTGCAGGGATGGATCTCAGCAAACGGGAATATTTTGTGCTTTGTTAAATCTCTTA GAAAGTGCGGAAACAGAAGAGGTAGTGGATATTTTTCAAGTGGTAAAAGCTCTACGCAAAGCTAGGCCAGGCATGGTTTCCACATTCGAGCAATATCAAT TCCTATATGACGTCATTGCCAGCACCTACCCTGCTCAGAATGGACAAGTAAAGAAAAACAACCATCAAGAAGATAAAATTGAATTTGATAATGAAGTGGA CAAAGTAAAGCAGGATGCTAATTGTGTTAATCCACTTGGTGCCCCAGAAAAGCTCCCTGAAGCAAAGGAACAGGCTGAAGGTTCTGAACCCACGAGTGGC ACTGAGGGGCCAGAACATTCTGTCAATGGTCCTGCAAGTCCAGCTTTAAATCAAGGTTCATAGGAAAAGACATAAATGAGGAAACTCCAAACCTCCTGTT AGCTGTTATTTCTATTTTTGTAGAAGTAGGAAGTGAAAATAGGTATACAGTGGATTAATTAAATGCAGCGAACCAATATTTGTAGAAGGGTTATATTTTA CTACTGTGGAAAAATATTTAAGATAGTTTTGCCAGAACAGTTTGTACAGACGTATGCTTATTTTAAAATTTTATCTCTTATTCAGTAAAAAACAACTTCT TTGTAATCGTTATGTGTGTATATGTATGTGTGTATGGGTGTGTGTTTGTGTGAGAGACAGAGAAAGAGAGAGAATTCTTTCAAGTGAATCTAAAAGCTTT TGCTTTTCCTTTGTTTTTATGAAGAAAAAATACATTTTATATTAGAAGTGTTAACTTAGCTTGAAGGATCTGTTTTTAAAAATCATAAACTGTGTGCAGA CTCAATAAAATCATGTACATTTCTGAAATGACCTCAAGATGTCCTCCTTGTTCTACTCATATATATCTATCTTATATAGTTTACTATTTTACTTCTAGAG ATAGTACATAAAGGTGGTATGTGTGTGTATGCTACTACAAAAAAGTTGTTAACTAAATTAACATTGGGAAATCTTATATTCCATATATTAGCATTTAGTC CAATGTCTTTTTAAGCTTATTTAATTAAAAAATTTCCAGTGAGCTTATCATGCTGTCTTTACATGGGGTTTTCAATTTTGCATGCTCGATTATTCCCTGT ACAATATTTAAAATTTATTGCTTGATACTTTTGACAACAAATTAGGTTTTGTACAATTGAACTTAAATAAATGTCATTAAAATAAATAAATGCAATATGT ATTAATATTCATTGTATAAAAATAGAAGAATACAAACATATTTGTTAAATATTTACATATGAAATTTAATATAGCTATTTTTATGGAATTTTTCATTGAT ATGAAAAATATGATATTGCATATGCATAGTTCCCATGTTAAATCCCATTCATAACTTTCATTAAAGCATTTACTTTGAATTTC

refseq_PTPRC.F1 (forward primer)
refseq_PTPRC.R1 (reverse primer)
Single exon skipping

Frame 0

RGGNCSSSDKTTVEKGRMLFLRDTADFQI*PCI CGLNSWHLALPFWTQKYL*QGKAQHLPPLD*LQQ RCPVFHFQVTPYLLTPLHSHPQAPLKEKMTSQR PQLLLVQTILPPKYPRTLWIMLVLLIPQVFHQY RRLTFPRTQTRRRPLLELTRRHSAAPPPMQNSTL PQAAMLSQMPTLMPLKQPL*ALLEALSFQPQQ* LLLHLSQHVMKNMQTSLWITYITRKLNYLQQS* MLMRMWNVETILAQTMRCITLQNVKMRLFPYLII HVLLLIRH*Y*MCHQGLKSFSYMIVHKLKKQIL LFV*NGKILKPLLVIHRILPTDFSVVI*YLIIK KLN*KTLNPNMSISVTQKYSIITTSLLTQVKLLK QILGVQESLRLFFVEVKLHIKE*LPGIPLKDHF IILPSVI*KRQKKIASIWIKT*SNMICKI*NLI RNMFYHYMPTSLQKCNVMEVLQCVISQLKVLLQA RSGT*LSP*HQIIVCMSSVGLPGTVMAPMNVTI WKLKLEILWLEMSRIRIAISV*KIFNIQQTTLL RPIFTMETILENPLFYIIQHLIILRH**HFWHF* LL*HQ*PCLLFSTKSMIYIRKDPAI*MNSRSLL KGMMKNN**MWSQSMQIFCWKLIRGRLLMKEDF FWLNFRASRGCSASFL*RKLESPLTRIKTVMLTF FLMIITVLNSLR*TEMQGQTT*MPAILMVSKNP GNTLLHKVPGMKLLMISGG*FGNRKPQLLSWSL DVKKETGTSVQNTGRQWKRALGLLEMLL*RSTST KDVQITSFRN*TL*IKKKKQLEER*LTFSSPAG QTTGCLRILTCSSN*EGE*MPSAISSVVPLWCT AVLVLGAQEPISELMPC*KAWKPRTKWMFMVMLS S*GDRDA*WFK*RPSTS*SIRLWWNTISLEKQK *ICLNYIHIYIT*RKGIHPVSRLH*RLNSRDFL HIGAGGHSTLEIKKKIKVKTGILMSSHMTITECH LNMSWK*VKRVSMIQMNPLMMTVIQRNQANTSM HLL**ATGNLK**LLLRDH*RRPLVTFGR*SSK EKSKLLLC*QN*NMETRKSVLSTGEKESKHMEIL KLT*KTQTNLQLIPFVSLN*DIPRGKTLELCTS TNIQTGVWSSFLQNPRN*SL*FRSSNKNFPRRI PLKGTSITRVHLYSFTAGMDLSKREYFVLC*IS* KVRKQKR*WIFFKW*KLYAKLGQAWFPHSSNIN SYMTSLPAPTLLRMDK*RKTTIKKIKLNLIMKW TK*SRMLIVLIHLVPQKSSLKQRNRLKVLNPRVA LRGQNILSMVLQVQL*IKVHRKRHK*GNSKPPV SCYFYFCRSRK*K*VYSGLIKCSEPIFVEGLYF TTVEKYLR*FCQNSLYRRMLILKFYLLFSKKQLL CNRYVCICMCVWVCVCVRDRERERILSSESKSF CFSFVFMKKKYILY*KC*LSLKDLFLKIINCVQ TQ*NHVHF*NDLKMSSLFYSYISILYSLLFYF*R *YIKVVCVCMLLQKSC*LN*HWEILYSIY*HLV QCLFKLI*LKNFQ*AYHAVFTWGFQFCMLDYSL YNI*NLLLDTFDNKLGFVQLNLNKCH*NK*MQYV LIFIV*K*KNTNIFVKYLHMKFNIAIFMEFFID MKNMILHMHSSHVKSHS*LSLKHLL*I

Frame 1

EEEIVPRLIRQQWRKDACCFLGTRLTSRYDHVF VA*TLGIWLCLSGHRSICDRAKPNTFPHWIDYS KDAQCSTFK*PLTYSHHCILTRKHL*KRK*LLRD HNFS*SRQYFHPSIPGLFG*C*CF*YHRCFIST DASPSHARRLADALCWN*HADIQRLRRQCKTQP YPRQQCYLRCLP*CL*NNHSEPFWKRCHFNHNNS YYSI*ANM**KICKHHCGLLI*QGN*IIYSKAK C**ECGMWKQYLHKQ*GA*PYRM*KCVCFHIS* FMYCS**DINIRCATRG*KVSVT*LYTS*KSRYY YLFKMEKY*NLYL*YTEYYLQISVW*YDI***R N*IRKP*TRT*V*V*LRNTL**PQVY*RK*NY* NRFWESRRASDYFL*K*SCTSRSNYLESPSKIIS *FYPLLYKRDRKRLPQSG*KPDQI*FAKFKTLY EICFIITCLHHCKSAT*WKCCNVSFHN*KCSSK PGLEHDCLHDIR**YACQV*ASQGP*WPP*TLPF GS*SWKYSG*K*VA*ELRFPCKRSSIFNRLHF* GLFSQWRLSWRTLYFTSFNIL*F*GTDSISGIS DYCDINSPACCSLQNL*ST*EKILQFR*TAGAC* KG**KTTDECGANPCRYFVGNL*EEDC**RKTF SG*ISEHPAGVQQVSYKGSSKAL*PE*KPLC*H SSL*L*PC*TL*DKRRCRVKLHKCQLY*WFQRTQ EIHCCTRSQG*NC**FLEDDLGTESHSYCHGHS M*RRKQEQVCRILAVNGRGHSGFWRCCCKDQPA QKMSRLHHSEIEHCK*KRKSNWKRGDSHSVHQLA RPRGA*GSSLAPQTEKESECLQQFLQWSHCGAL QCWCWAHRNLYRN*CHARRPGSREQSGCLWLCC QAKATEMPDGSSRGPVHLDPSGFGGIQSVWRNRS EFV*ITSIST*HEEKGSTQ*AVSTRG*IPETSF I*ELEDTAHWKSRRK*K*KQEF*CHPI*L*QSA T*T*AGNE*RE*A*FR*IL***Q*FRGTKQIHQC IFYNELLET*SDDCCSGTTEGDHW*LLADDLPK KSQSYCYADRTETWRPGNLCSVLGRRKANIWRY *S*PERHRQIFNLYPSCL*TETFQEERLSNCVPV PIYKLECGAASCRTQGINLYDSGRQTKTSPEEF L*REQASQEYTSTHSLQGWISANGNILCFVKSL RKCGNRRGSGYFSSGKSSTQS*ARHGFHIRAISI PI*RHCQHLPCSEWTSKEKQPSRR*N*I***SG QSKAGC*LC*STWCPRKAP*SKGTG*RF*THEW H*GARTFCQWSCKSSFKSRFIGKDINEETPNLLL AVISIFVEVGSENRYTVD*LNAANQYL*KGYIL LLWKNI*DSFARTVCTDVCLF*NFISYSVKNNF FVIVMCVYVCVYGCVFV*ETEKEREFFQVNLKAF AFPLFL*RKNTFYIRSVNLA*RICF*KS*TVCR LNKIMYISEMTSRCPPCSTHIYLSYIVYYFTSR DST*RWYVCVCYYKKVVN*INIGKSYIPYISI*S NVFLSLFN*KISSELIMLSLHGVFNFACSIIPC TIFKIYCLILLTTN*VLYN*T*INVIKINKCNM Y*YSLYKNRRIQTYLLNIYI*NLI*LFLWNFSLI *KI*YCICIVPMLNPIHNFH*SIYFEF

Frame 2

RRKLFLV**DNSGERTHAVS*GHG*LPDMTMYL WLKLLAFGFAFLDTEVFVTGQSPTPSPTGLTTA KMPSVPLSSDPLPTHTTAFSPASTFERENDFSE TTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQ TPHLPTHADSQTPSAGTDTQTFSGSAANAKLNP TPGSNAISDAYLNASETTTLSPSGSAVISTTTI ATTPSKPTCDEKYANITVDYLYNKETKLFTAKLN VNENVECGNNTCTNNEVHNLTECKNASVSISHN SCTAPDKTLILDVPPGVEKFQLHDCTQVEKADT TICLKWKNIETFTCDTQNITYRFQCGNMIFDNKE IKLENLEPEHEYKCDSEILYNNHKFTNASKIIK TDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSF HNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYT KYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPS QVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYH LEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFK AYFHNGDYPGEPFILHHSTSYNSKALIAFLAFL IIVTSIALLVVLYKIYDLHKKRSCNLDEQQELV ERDDEKQLMNVEPIHADILLETYKRKIADEGRLF LAEFQSIPRVFSKFPIKEARKPFNQNKNRYVDI LPYDYNRVELSEINGDAGSNYINASYIDGFKEP RKYIAAQGPRDETVDDFWRMIWEQKATVIVMVTR CEEGNRNKCAEYWPSMEEGTRAFGDVVVKINQH KRCPDYIIQKLNIVNKKEKATGREVTHIQFTSW PDHGVPEDPHLLLKLRRRVNAFSNFFSGPIVVHC SAGVGRTGTYIGIDAMLEGLEAENKVDVYGYVV KLRRQRCLMVQVEAQYILIHQALVEYNQFGETE VNLSELHPYLHNMKKRDPPSEPSPLEAEFQRLPS YRSWRTQHIGNQEENKSKNRNSNVIPYDYNRVP LKHELEMSKESEHDSDESSDDDSDSEEPSKYIN ASFIMSYWKPEVMIAAQGPLKETIGDFWQMIFQR KVKVIVMLTELKHGDQEICAQYWGEGKQTYGDI EVDLKDTDKSSTYTLRVFELRHSKRKDSRTVYQ YQYTNWSVEQLPAEPKELISMIQVVKQKLPQKNS SEGNKHHKSTPLLIHCRDGSQQTGIFCALLNLL ESAETEEVVDIFQVVKALRKARPGMVSTFEQYQ FLYDVIASTYPAQNGQVKKNNHQEDKIEFDNEVD KVKQDANCVNPLGAPEKLPEAKEQAEGSEPTSG TEGPEHSVNGPASPALNQGS*EKT*MRKLQTSC *LLFLFL*K*EVKIGIQWIN*MQRTNICRRVIFY YCGKIFKIVLPEQFVQTYAYFKILSLIQ*KTTS L*SLCVYMYVCMGVCLCERQRKRENSFK*I*KL LLFLCFYEEKIHFILEVLT*LEGSVFKNHKLCAD SIKSCTFLK*PQDVLLVLLIYIYLI*FTILLLE IVHKGGMCVYATTKKLLTKLTLGNLIFHILAFS PMSF*AYLIKKFPVSLSCCLYMGFSILHARLFPV QYLKFIA*YF*QQIRFCTIELK*MSLK*INAIC INIHCIKIEEYKHIC*IFTYEI*YSYFYGIFH* YEKYDIAYA*FPC*IPFITFIKAFTLNF

Sequence NM_002838

AGAGGAGGAAATTGTTCCTCGTCTGATAAGACAACAGTGGAGAAAGGACGCATGCTGTTTCTTAGGGACACGGCTGACTTCCAGATATGACCATGTATTT GTGGCTTAAACTCTTGGCATTTGGCTTTGCCTTTCTGGACACAGAAGTATTTGTGACAGGGCAAAGCCCAACACCTTCCCCCACTGGATTGACTACAGCA AAGATGCCCAGTGTTCCACTTTCAAGTGACCCCTTACCTACTCACACCACTGCATTCTCACCCGCAAGCACCTTTGAAAGAGAAAATGACTTCTCAGAGA CCACAACTTCTCTTAGTCCAGACAATACTTCCACCCAAGTATCCCCGGACTCTTTGGATAATGCTAGTGCTTTTAATACCACAGGTGTTTCATCAGTACA GACGCCTCACCTTCCCACGCACGCAGACTCGCAGACGCCCTCTGCTGGAACTGACACGCAGACATTCAGCGGCTCCGCCGCCAATGCAAAACTCAACCCT ACCCCAGGCAGCAATGCTATCTCAGATGTCCCAGGAGAGAGGAGTACAGCCAGCACCTTTCCTACAGACCCAGTTTCCCCATTGACAACCACCCTCAGCC TTGCACACCACAGCTCTGCTGCCTTACCTGCACGCACCTCCAACACCACCATCACAGCGAACACCTCAGATGCCTACCTTAATGCCTCTGAAACAACCAC TCTGAGCCCTTCTGGAAGCGCTGTCATTTCAACCACAACAATAGCTACTACTCCATCTAAGCCAACATGTGATGAAAAATATGCAAACATCACTGTGGAT TACTTATATAACAAGGAAACTAAATTATTTACAGCAAAGCTAAATGTTAATGAGAATGTGGAATGTGGAAACAATACTTGCACAAACAATGAGGTGCATA ACCTTACAGAATGTAAAAATGCGTCTGTTTCCATATCTCATAATTCATGTACTGCTCCTGATAAGACATTAATATTAGATGTGCCACCAGGGGTTGAAAA GTTTCAGTTACATGATTGTACACAAGTTGAAAAAGCAGATACTACTATTTGTTTAAAATGGAAAAATATTGAAACCTTTACTTGTGATACACAGAATATT ACCTACAGATTTCAGTGTGGTAATATGATATTTGATAATAAAGAAATTAAATTAGAAAACCTTGAACCCGAACATGAGTATAAGTGTGACTCAGAAATAC TCTATAATAACCACAAGTTTACTAACGCAAGTAAAATTATTAAAACAGATTTTGGGAGTCCAGGAGAGCCTCAGATTATTTTTTGTAGAAGTGAAGCTGC ACATCAAGGAGTAATTACCTGGAATCCCCCTCAAAGATCATTTCATAATTTTACCCTCTGTTATATAAAAGAGACAGAAAAAGATTGCCTCAATCTGGAT AAAAACCTGATCAAATATGATTTGCAAAATTTAAAACCTTATACGAAATATGTTTTATCATTACATGCCTACATCATTGCAAAAGTGCAACGTAATGGAA GTGCTGCAATGTGTCATTTCACAACTAAAAGTGCTCCTCCAAGCCAGGTCTGGAACATGACTGTCTCCATGACATCAGATAATAGTATGCATGTCAAGTG TAGGCCTCCCAGGGACCGTAATGGCCCCCATGAACGTTACCATTTGGAAGTTGAAGCTGGAAATACTCTGGTTAGAAATGAGTCGCATAAGAATTGCGAT TTCCGTGTAAAAGATCTTCAATATTCAACAGACTACACTTTTAAGGCCTATTTTCACAATGGAGACTATCCTGGAGAACCCTTTATTTTACATCATTCAA CATCTTATAATTCTAAGGCACTGATAGCATTTCTGGCATTTCTGATTATTGTGACATCAATAGCCCTGCTTGTTGTTCTCTACAAAATCTATGATCTACA TAAGAAAAGATCCTGCAATTTAGATGAACAGCAGGAGCTTGTTGAAAGGGATGATGAAAAACAACTGATGAATGTGGAGCCAATCCATGCAGATATTTTG TTGGAAACTTATAAGAGGAAGATTGCTGATGAAGGAAGACTTTTTCTGGCTGAATTTCAGAGCATCCCGCGGGTGTTCAGCAAGTTTCCTATAAAGGAAG CTCGAAAGCCCTTTAACCAGAATAAAAACCGTTATGTTGACATTCTTCCTTATGATTATAACCGTGTTGAACTCTCTGAGATAAACGGAGATGCAGGGTC AAACTACATAAATGCCAGCTATATTGATGGTTTCAAAGAACCCAGGAAATACATTGCTGCACAAGGTCCCAGGGATGAAACTGTTGATGATTTCTGGAGG ATGATTTGGGAACAGAAAGCCACAGTTATTGTCATGGTCACTCGATGTGAAGAAGGAAACAGGAACAAGTGTGCAGAATACTGGCCGTCAATGGAAGAGG GCACTCGGGCTTTTGGAGATGTTGTTGTAAAGATCAACCAGCACAAAAGATGTCCAGATTACATCATTCAGAAATTGAACATTGTAAATAAAAAAGAAAA AGCAACTGGAAGAGAGGTGACTCACATTCAGTTCACCAGCTGGCCAGACCACGGGGTGCCTGAGGATCCTCACTTGCTCCTCAAACTGAGAAGGAGAGTG AATGCCTTCAGCAATTTCTTCAGTGGTCCCATTGTGGTGCACTGCAGTGCTGGTGTTGGGCGCACAGGAACCTATATCGGAATTGATGCCATGCTAGAAG GCCTGGAAGCCGAGAACAAAGTGGATGTTTATGGTTATGTTGTCAAGCTAAGGCGACAGAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGAT CCATCAGGCTTTGGTGGAATACAATCAGTTTGGAGAAACAGAAGTGAATTTGTCTGAATTACATCCATATCTACATAACATGAAGAAAAGGGATCCACCC AGTGAGCCGTCTCCACTAGAGGCTGAATTCCAGAGACTTCCTTCATATAGGAGCTGGAGGACACAGCACATTGGAAATCAAGAAGAAAATAAAAGTAAAA ACAGGAATTCTAATGTCATCCCATATGACTATAACAGAGTGCCACTTAAACATGAGCTGGAAATGAGTAAAGAGAGTGAGCATGATTCAGATGAATCCTC TGATGATGACAGTGATTCAGAGGAACCAAGCAAATACATCAATGCATCTTTTATAATGAGCTACTGGAAACCTGAAGTGATGATTGCTGCTCAGGGACCA CTGAAGGAGACCATTGGTGACTTTTGGCAGATGATCTTCCAAAGAAAAGTCAAAGTTATTGTTATGCTGACAGAACTGAAACATGGAGACCAGGAAATCT GTGCTCAGTACTGGGGAGAAGGAAAGCAAACATATGGAGATATTGAAGTTGACCTGAAAGACACAGACAAATCTTCAACTTATACCCTTCGTGTCTTTGA ACTGAGACATTCCAAGAGGAAAGACTCTCGAACTGTGTACCAGTACCAATATACAAACTGGAGTGTGGAGCAGCTTCCTGCAGAACCCAAGGAATTAATC TCTATGATTCAGGTCGTCAAACAAAAACTTCCCCAGAAGAATTCCTCTGAAGGGAACAAGCATCACAAGAGTACACCTCTACTCATTCACTGCAGGGATG GATCTCAGCAAACGGGAATATTTTGTGCTTTGTTAAATCTCTTAGAAAGTGCGGAAACAGAAGAGGTAGTGGATATTTTTCAAGTGGTAAAAGCTCTACG CAAAGCTAGGCCAGGCATGGTTTCCACATTCGAGCAATATCAATTCCTATATGACGTCATTGCCAGCACCTACCCTGCTCAGAATGGACAAGTAAAGAAA AACAACCATCAAGAAGATAAAATTGAATTTGATAATGAAGTGGACAAAGTAAAGCAGGATGCTAATTGTGTTAATCCACTTGGTGCCCCAGAAAAGCTCC CTGAAGCAAAGGAACAGGCTGAAGGTTCTGAACCCACGAGTGGCACTGAGGGGCCAGAACATTCTGTCAATGGTCCTGCAAGTCCAGCTTTAAATCAAGG TTCATAGGAAAAGACATAAATGAGGAAACTCCAAACCTCCTGTTAGCTGTTATTTCTATTTTTGTAGAAGTAGGAAGTGAAAATAGGTATACAGTGGATT AATTAAATGCAGCGAACCAATATTTGTAGAAGGGTTATATTTTACTACTGTGGAAAAATATTTAAGATAGTTTTGCCAGAACAGTTTGTACAGACGTATG CTTATTTTAAAATTTTATCTCTTATTCAGTAAAAAACAACTTCTTTGTAATCGTTATGTGTGTATATGTATGTGTGTATGGGTGTGTGTTTGTGTGAGAG ACAGAGAAAGAGAGAGAATTCTTTCAAGTGAATCTAAAAGCTTTTGCTTTTCCTTTGTTTTTATGAAGAAAAAATACATTTTATATTAGAAGTGTTAACT TAGCTTGAAGGATCTGTTTTTAAAAATCATAAACTGTGTGCAGACTCAATAAAATCATGTACATTTCTGAAATGACCTCAAGATGTCCTCCTTGTTCTAC TCATATATATCTATCTTATATAGTTTACTATTTTACTTCTAGAGATAGTACATAAAGGTGGTATGTGTGTGTATGCTACTACAAAAAAGTTGTTAACTAA ATTAACATTGGGAAATCTTATATTCCATATATTAGCATTTAGTCCAATGTCTTTTTAAGCTTATTTAATTAAAAAATTTCCAGTGAGCTTATCATGCTGT CTTTACATGGGGTTTTCAATTTTGCATGCTCGATTATTCCCTGTACAATATTTAAAATTTATTGCTTGATACTTTTGACAACAAATTAGGTTTTGTACAA TTGAACTTAAATAAATGTCATTAAAATAAATAAATGCAATATGTATTAATATTCATTGTATAAAAATAGAAGAATACAAACATATTTGTTAAATATTTAC ATATGAAATTTAATATAGCTATTTTTATGGAATTTTTCATTGATATGAAAAATATGATATTGCATATGCATAGTTCCCATGTTAAATCCCATTCATAACT TTCATTAAAGCATTTACTTTGAATTTC

refseq_PTPRC.F1 (forward primer)
refseq_PTPRC.R1 (reverse primer)
Single exon skipping

Frame 0

RGGNCSSSDKTTVEKGRMLFLRDTADFQI*PCI CGLNSWHLALPFWTQKYL*QGKAQHLPPLD*LQQ RCPVFHFQVTPYLLTPLHSHPQAPLKEKMTSQR PQLLLVQTILPPKYPRTLWIMLVLLIPQVFHQY RRLTFPRTQTRRRPLLELTRRHSAAPPPMQNSTL PQAAMLSQMSQERGVQPAPFLQTQFPH*QPPSA LHTTALLPYLHAPPTPPSQRTPQMPTLMPLKQP L*ALLEALSFQPQQ*LLLHLSQHVMKNMQTSLWI TYITRKLNYLQQS*MLMRMWNVETILAQTMRCI TLQNVKMRLFPYLIIHVLLLIRH*Y*MCHQGLK SFSYMIVHKLKKQILLFV*NGKILKPLLVIHRIL PTDFSVVI*YLIIKKLN*KTLNPNMSISVTQKY SIITTSLLTQVKLLKQILGVQESLRLFFVEVKL HIKE*LPGIPLKDHFIILPSVI*KRQKKIASIWI KT*SNMICKI*NLIRNMFYHYMPTSLQKCNVME VLQCVISQLKVLLQARSGT*LSP*HQIIVCMSS VGLPGTVMAPMNVTIWKLKLEILWLEMSRIRIAI SV*KIFNIQQTTLLRPIFTMETILENPLFYIIQ HLIILRH**HFWHF*LL*HQ*PCLLFSTKSMIY IRKDPAI*MNSRSLLKGMMKNN**MWSQSMQIFC WKLIRGRLLMKEDFFWLNFRASRGCSASFL*RK LESPLTRIKTVMLTFFLMIITVLNSLR*TEMQG QTT*MPAILMVSKNPGNTLLHKVPGMKLLMISGG *FGNRKPQLLSWSLDVKKETGTSVQNTGRQWKR ALGLLEMLL*RSTSTKDVQITSFRN*TL*IKKK KQLEER*LTFSSPAGQTTGCLRILTCSSN*EGE* MPSAISSVVPLWCTAVLVLGAQEPISELMPC*K AWKPRTKWMFMVMLSS*GDRDA*WFK*RPSTS* SIRLWWNTISLEKQK*ICLNYIHIYIT*RKGIHP VSRLH*RLNSRDFLHIGAGGHSTLEIKKKIKVK TGILMSSHMTITECHLNMSWK*VKRVSMIQMNP LMMTVIQRNQANTSMHLL**ATGNLK**LLLRDH *RRPLVTFGR*SSKEKSKLLLC*QN*NMETRKS VLSTGEKESKHMEILKLT*KTQTNLQLIPFVSL N*DIPRGKTLELCTSTNIQTGVWSSFLQNPRN*S L*FRSSNKNFPRRIPLKGTSITRVHLYSFTAGM DLSKREYFVLC*IS*KVRKQKR*WIFFKW*KLY AKLGQAWFPHSSNINSYMTSLPAPTLLRMDK*RK TTIKKIKLNLIMKWTK*SRMLIVLIHLVPQKSS LKQRNRLKVLNPRVALRGQNILSMVLQVQL*IK VHRKRHK*GNSKPPVSCYFYFCRSRK*K*VYSGL IKCSEPIFVEGLYFTTVEKYLR*FCQNSLYRRM LILKFYLLFSKKQLLCNRYVCICMCVWVCVCVR DRERERILSSESKSFCFSFVFMKKKYILY*KC*L SLKDLFLKIINCVQTQ*NHVHF*NDLKMSSLFY SYISILYSLLFYF*R*YIKVVCVCMLLQKSC*L N*HWEILYSIY*HLVQCLFKLI*LKNFQ*AYHAV FTWGFQFCMLDYSLYNI*NLLLDTFDNKLGFVQ LNLNKCH*NK*MQYVLIFIV*K*KNTNIFVKYL HMKFNIAIFMEFFIDMKNMILHMHSSHVKSHS*L SLKHLL*I

Frame 1

EEEIVPRLIRQQWRKDACCFLGTRLTSRYDHVF VA*TLGIWLCLSGHRSICDRAKPNTFPHWIDYS KDAQCSTFK*PLTYSHHCILTRKHL*KRK*LLRD HNFS*SRQYFHPSIPGLFG*C*CF*YHRCFIST DASPSHARRLADALCWN*HADIQRLRRQCKTQP YPRQQCYLRCPRREEYSQHLSYRPSFPIDNHPQP CTPQLCCLTCTHLQHHHHSEHLRCLP*CL*NNH SEPFWKRCHFNHNNSYYSI*ANM**KICKHHCG LLI*QGN*IIYSKAKC**ECGMWKQYLHKQ*GA* PYRM*KCVCFHIS*FMYCS**DINIRCATRG*K VSVT*LYTS*KSRYYYLFKMEKY*NLYL*YTEY YLQISVW*YDI***RN*IRKP*TRT*V*V*LRNT L**PQVY*RK*NY*NRFWESRRASDYFL*K*SC TSRSNYLESPSKIIS*FYPLLYKRDRKRLPQSG *KPDQI*FAKFKTLYEICFIITCLHHCKSAT*WK CCNVSFHN*KCSSKPGLEHDCLHDIR**YACQV *ASQGP*WPP*TLPFGS*SWKYSG*K*VA*ELR FPCKRSSIFNRLHF*GLFSQWRLSWRTLYFTSFN IL*F*GTDSISGISDYCDINSPACCSLQNL*ST *EKILQFR*TAGAC*KG**KTTDECGANPCRYF VGNL*EEDC**RKTFSG*ISEHPAGVQQVSYKGS SKAL*PE*KPLC*HSSL*L*PC*TL*DKRRCRV KLHKCQLY*WFQRTQEIHCCTRSQG*NC**FLE DDLGTESHSYCHGHSM*RRKQEQVCRILAVNGRG HSGFWRCCCKDQPAQKMSRLHHSEIEHCK*KRK SNWKRGDSHSVHQLARPRGA*GSSLAPQTEKES ECLQQFLQWSHCGALQCWCWAHRNLYRN*CHARR PGSREQSGCLWLCCQAKATEMPDGSSRGPVHLD PSGFGGIQSVWRNRSEFV*ITSIST*HEEKGST Q*AVSTRG*IPETSFI*ELEDTAHWKSRRK*K*K QEF*CHPI*L*QSAT*T*AGNE*RE*A*FR*IL ***Q*FRGTKQIHQCIFYNELLET*SDDCCSGT TEGDHW*LLADDLPKKSQSYCYADRTETWRPGNL CSVLGRRKANIWRY*S*PERHRQIFNLYPSCL* TETFQEERLSNCVPVPIYKLECGAASCRTQGIN LYDSGRQTKTSPEEFL*REQASQEYTSTHSLQGW ISANGNILCFVKSLRKCGNRRGSGYFSSGKSST QS*ARHGFHIRAISIPI*RHCQHLPCSEWTSKE KQPSRR*N*I***SGQSKAGC*LC*STWCPRKAP *SKGTG*RF*THEWH*GARTFCQWSCKSSFKSR FIGKDINEETPNLLLAVISIFVEVGSENRYTVD *LNAANQYL*KGYILLLWKNI*DSFARTVCTDVC LF*NFISYSVKNNFFVIVMCVYVCVYGCVFV*E TEKEREFFQVNLKAFAFPLFL*RKNTFYIRSVN LA*RICF*KS*TVCRLNKIMYISEMTSRCPPCST HIYLSYIVYYFTSRDST*RWYVCVCYYKKVVN* INIGKSYIPYISI*SNVFLSLFN*KISSELIML SLHGVFNFACSIIPCTIFKIYCLILLTTN*VLYN *T*INVIKINKCNMY*YSLYKNRRIQTYLLNIY I*NLI*LFLWNFSLI*KI*YCICIVPMLNPIHN FH*SIYFEF

Frame 2

RRKLFLV**DNSGERTHAVS*GHG*LPDMTMYL WLKLLAFGFAFLDTEVFVTGQSPTPSPTGLTTA KMPSVPLSSDPLPTHTTAFSPASTFERENDFSE TTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQ TPHLPTHADSQTPSAGTDTQTFSGSAANAKLNP TPGSNAISDVPGERSTASTFPTDPVSPLTTTLS LAHHSSAALPARTSNTTITANTSDAYLNASETTT LSPSGSAVISTTTIATTPSKPTCDEKYANITVD YLYNKETKLFTAKLNVNENVECGNNTCTNNEVH NLTECKNASVSISHNSCTAPDKTLILDVPPGVEK FQLHDCTQVEKADTTICLKWKNIETFTCDTQNI TYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEI LYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAA HQGVITWNPPQRSFHNFTLCYIKETEKDCLNLD KNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNG SAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKC RPPRDRNGPHERYHLEVEAGNTLVRNESHKNCD FRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHS TSYNSKALIAFLAFLIIVTSIALLVVLYKIYDLH KKRSCNLDEQQELVERDDEKQLMNVEPIHADIL LETYKRKIADEGRLFLAEFQSIPRVFSKFPIKE ARKPFNQNKNRYVDILPYDYNRVELSEINGDAGS NYINASYIDGFKEPRKYIAAQGPRDETVDDFWR MIWEQKATVIVMVTRCEEGNRNKCAEYWPSMEE GTRAFGDVVVKINQHKRCPDYIIQKLNIVNKKEK ATGREVTHIQFTSWPDHGVPEDPHLLLKLRRRV NAFSNFFSGPIVVHCSAGVGRTGTYIGIDAMLE GLEAENKVDVYGYVVKLRRQRCLMVQVEAQYILI HQALVEYNQFGETEVNLSELHPYLHNMKKRDPP SEPSPLEAEFQRLPSYRSWRTQHIGNQEENKSK NRNSNVIPYDYNRVPLKHELEMSKESEHDSDESS DDDSDSEEPSKYINASFIMSYWKPEVMIAAQGP LKETIGDFWQMIFQRKVKVIVMLTELKHGDQEI CAQYWGEGKQTYGDIEVDLKDTDKSSTYTLRVFE LRHSKRKDSRTVYQYQYTNWSVEQLPAEPKELI SMIQVVKQKLPQKNSSEGNKHHKSTPLLIHCRD GSQQTGIFCALLNLLESAETEEVVDIFQVVKALR KARPGMVSTFEQYQFLYDVIASTYPAQNGQVKK NNHQEDKIEFDNEVDKVKQDANCVNPLGAPEKL PEAKEQAEGSEPTSGTEGPEHSVNGPASPALNQG S*EKT*MRKLQTSC*LLFLFL*K*EVKIGIQWI N*MQRTNICRRVIFYYCGKIFKIVLPEQFVQTY AYFKILSLIQ*KTTSL*SLCVYMYVCMGVCLCER QRKRENSFK*I*KLLLFLCFYEEKIHFILEVLT *LEGSVFKNHKLCADSIKSCTFLK*PQDVLLVL LIYIYLI*FTILLLEIVHKGGMCVYATTKKLLTK LTLGNLIFHILAFSPMSF*AYLIKKFPVSLSCC LYMGFSILHARLFPVQYLKFIA*YF*QQIRFCT IELK*MSLK*INAICINIHCIKIEEYKHIC*IFT YEI*YSYFYGIFH*YEKYDIAYA*FPC*IPFIT FIKAFTLNF