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Sequences

Sequence NM_001005476

TCCGGGGCTGAGTCCGCGTCGACGCCGGCCGCGGAGGCGGCACCATGGGCAAGGGGTAGAGGGGCAAGTTGGCCACCGCCGCCGCCGGGGGTGGTGGGAG AGCCGCTCCGGGGGCGGGGGCCGGTGGGGGAGGGAGGGGCGGGCAGCCGCGCCGCCGCGGCACTTTTTTAATTTTTTCGGGTGCCGCAGCGGCGACCCCT CGGCGCCGATGTCCCTGATCCCTGGAGCGACGACGGCCGCTGCCTAAGCTGGAAAGAGGAATGCCAGCTCCTGAGCAGGCCTCATTGGTGGAGGAGGGGC AACCACAGACCCGCCAGGAAGCTGCCTCCACTGGCCCAGGCATGGAACCCGAGACCACAGCCACCACTATTCTAGCATCCGTGAAGGAGCAGGAGCTTCA GTTTCAGCGACTCACCCGAGAACTGGAAGTGGAAAGGCAGATTGTTGCCAGTCAGCTAGAAAGATGTAGGCTTGGAGCAGAATCACCAAGCATCGCCAGC ACCAGCTCAACTGAGAAGTCATTTCCTTGGAGATCAACAGACGTGCCAAATACTGGTGTAAGCAAACCTAGAGTTTCTGACGCTGTCCAGCCCAACAACT ATCTCATCAGGACAGAGCCAGAACAAGGAACCCTCTATTCACCAGAACAGACATCTCTCCATGAAAGTGAGGGATCATTGGGTAACTCAAGAAGTTCAAC ACAAATGAATTCTTATTCCGACAGTGGATACCAGGAAGCAGGGAGTTTCCACAACAGCCAGAACGTGAGCAAGGCAGACAACAGACAGCAGCATTCATTC ATAGGATCAACTAACAACCATGTGGTGAGGAATTCAAGAGCTGAAGGACAAACACTGGTTCAGCCATCAGTAGCCAATCGGGCCATGAGAAGAGTTAGTT CAGTTCCATCTAGAGCACAGTCTCCTTCTTATGTTATCAGCACAGGCGTGTCTCCTTCAAGGGGGTCTCTGAGAACTTCTCTGGGTAGTGGATTTGGCTC TCCGTCAGTGACCGACCCCCGACCTCTGAACCCCAGTGCATATTCCTCCACCACATTACCTGCTGCACGGGCAGCCTCTCCGTACTCACAGAGACCCGCC TCCCCAACAGCTATACGGCGGATTGGGTCAGTCACCTCCCGGCAGACCTCCAATCCCAACGGACCAACCCCTCAATACCAAACCACCGCCAGAGTGGGGT CCCCACTGACCCTGACGGATGCACAGACTCGAGTAGCTTCCCCATCCCAAGGCCAGGTGGGGTCGTCGTCCCCCAAACGCTCAGGGATGACCGCCGTACC ACAGCATCTGGGACCTTCACTGCAAAGGACTGTTCATGACATGGAGCAATTCGGACAGCAGCAGTATGACATTTATGAGAGGATGGTTCCACCCAGGCCA GACAGCCTGACAGGCTTACGGAGTTCCTATGCTAGTCAGCATAGTCAGCTTGGGCAAGACCTTCGTTCTGCCGTGTCTCCCGACTTGCACATTACTCCTA TATATGAGGGGAGGACCTATTACAGCCCAGTGTACCGCAGCCCAAACCATGGAACTGTGGAGCTCCAAGGATCGCAGACGGCGTTGTATCGCACAGGTTC AGTAGGTATTGGAAATCTACAAAGGACATCCAGCCAACGAAGTACCCTTACATACCAAAGAAATAATTATGCTCTGAACACAACAGCTACCTACGCGGAG CCCTACAGGCCTATACAATACCGAGTGCAAGAGTGCAATTATAACAGGCTTCAGCATGCAGTGCCGGCTGATGATGGCACCACAAGATCCCCATCAATAG ACAGCATTCAGAAGGACCCCAGGGAGTTTGCCTGGCGTGATCCTGAGTTGCCTGAGGTCATTCACATGCTTCAGCACCAGTTCCCATCTGTTCAGGCAAA TGCAGCGGCCTACCTGCAGCACCTGTGCTTTGGTGACAACAAAGTGAAGATGGAGGTGTGTAGGTTAGGGGGAATCAAGCATCTGGTTGACCTTCTGGAC CACAGAGTTTTGGAAGTTCAGAAGAATGCTTGTGGTGCCCTTCGAAACCTCGTTTTTGGCAAGTCTACAGATGAAAATAAAATAGCAATGAAGAATGTTG GTGGGATACCTGCCTTGTTGCGACTGTTGAGAAAATCTATTGATGCAGAAGTAAGGGAGCTTGTTACAGGAGTTCTTTGGAATTTATCCTCATGTGATGC TGTAAAAATGACAATCATTCGAGATGCTCTCTCAACCTTAACAAACACTGTGATTGTTCCACATTCTGGATGGAATAACTCTTCTTTTGATGATGATCAT AAAATTAAATTTCAGACTTCACTAGTTCTGCGTAACACGACAGGTTGCCTAAGGAACCTCAGCTCCGCGGGGGAAGAAGCTCGGAAGCAAATGCGGTCCT GCGAGGGGCTGGTAGACTCACTGTTGTATGTGATCCACACGTGTGTGAACACATCCGATTACGACAGCAAGACGGTGGAGAACTGCGTGTGCACCCTGAG GAACCTGTCCTATCGGCTGGAGCTGGAGGTGCCCCAGGCCCGGTTACTGGGACTGAACGAATTGGATGACTTACTAGGAAAAGAGTCTCCCAGCAAAGAC TCTGAGCCAAGTTGCTGGGGGAAGAAGAAGAAAAAGAAAAAGAGGACTCCGCAAGAAGATCAATGGGATGGAGTTGGTCCTATCCCAGGACTGTCGAAGT CCCCCAAAGGGGTTGAGATGCTGTGGCACCCATCGGTGGTAAAACCATATCTGACTCTTCTAGCAGAAAGTTCCAACCCAGCCACCTTGGAAGGCTCTGC AGGGTCTCTCCAGAACCTCTCTGCTGGCAACTGGAAGTTTGCAGCATATATCCGGGCGGCCGTCCGAAAAGAAAAGGGGCTCCCCATCCTTGTGGAGCTT CTGAGAATGGATAACGATAGAGTTGTTTCTTCCGTGGCAACAGCCTTGAGGAATATGGCACTAGATGTTCGCAACAAGGAGCTCATAGGCAAATACGCCA TGCGAGACCTGGTCAACCGGCTCCCCGGCGGCAATGGCCCCAGTGTCTTGTCTGATGAGACCATGGCAGCCATCTGCTGTGCTCTGCACGAGGTCACCAG CAAAAACATGGAGAACGCAAAAGCCCTGGCCGACTCAGGAGGCATAGAGAAGCTGGTGAACATAACCAAAGGCAGGGGCGACAGATCATCTCTGAAAGTG GTGAAGGCAGCAGCCCAGGTCTTGAATACATTATGGCAATATCGGGACCTCCGGAGCATTTATAAAAAGGATGGGTGGAATCAGAACCATTTTATTACAC CTGTGTCGACATTGGAGCGAGACCGATTCAAATCACATCCTTCCTTGTCTACCACCAACCAACAGATGTCACCCATCATTCAGTCAGGCTCCAGCAAACC TTCACCAATTTACATCAGTTCCTATTCCTCACCAGCAAGAGAACAAAATAGACGGCTACAGCATCAACAGCTGTATTATAGTCAAGATGACTCCAACAGA AAGAACTTTGATGCATACAGATTGTATTTGCAGTCTCCTCATAGCTATGAAGATCCTTATTTTGATGACCGAGTTCACTTTCCAGCTTCTACTGATTACT CAACACAGTATGGACTGAAATCGACCACAAATTATGTAGACTTTTATTCCACTAAACGACCTTCTTATAGAGCAGAACAGTACCCAGGGTCCCCAGACTC ATGGGTGTAGCATCAAGATGCCCAACAGAGGAACTCTTTCTTTCTAACCTTGTTCAGATTGAGGTGAAAAGTCCATCTTGCTGATTTGATGATTGAAATG TGAAAGTGAAGTGGAAGGAATGAATGAAGTGTGTTTTTTTTTTCTTTTTTGAGGAATTATCAGGGAAGTGAGGAAATGTTTGGGAGAGGACTTTCTAAGC TCTATTTAGGTGTTAGATCTAATTACTTATAGATTCTGTAGTCTGGTGAAGGTGTGGGTGACGTGATGAGAGGTTTGAGAAATGGGTGAAATGAAATGGG GGATATGTAGGTCAAATCAAATTAAAGATGATTTTTTTAATGTGAATAAAGTTATGTTCTGATAGTTTGTACAGAAAAAATAAAATGGATGCCCATGTTT TATTGCTATTACTAAATGTCAAGATTGTATGCTATTATGTCTTGTAAATTTCTTTTGTTGGTGTAAATATGGAAATGCCACATTGGTTAAGTGCCATCAT TTGTAATGCAATGTGTCACTTGAAAAGAGATTTGAAGAAACTGACAACTTCAAAAACAAATGAGAAGCCCAAGGAACTGTGAGCAATTAAAAGCAAACCG CGACACCCTTTGTCTCCACCACACATAGTGTACTTTGGAAGCACAACGTCCAGGCTGGTACCGCAGCGCCATGCCCATTCCTCGCCTCATTCATAGGACA CTTCACTGCCATTTTCTATTCACATAAAAGAAAAATAAATGTGGAAATTTCATCCTTGGAAA

refseq_PKP4.F2 (forward primer)
refseq_PKP4.R2 (reverse primer)
Single exon skipping

Frame 0

SGAESASTPAAEAAPWARGRGASWPPPPPGVVG EPLRGRGPVGEGGAGSRAAAALF*FFRVPQRRPL GADVPDPWSDDGRCLSWKEECQLLSRPHWWRRG NHRPARKLPPLAQAWNPRPQPPLF*HP*RSRSF SFSDSPENWKWKGRLLPVS*KDVGLEQNHQASPA PAQLRSHFLGDQQTCQILV*ANLEFLTLSSPTT ISSGQSQNKEPSIHQNRHLSMKVRDHWVTQEVQ HK*ILIPTVDTRKQGVSTTART*ARQTTDSSIHS *DQLTTMW*GIQELKDKHWFSHQ*PIGP*EELV QFHLEHSLLLMLSAQACLLQGGL*ELLWVVDLA LRQ*PTPDL*TPVHIPPPHYLLHGQPLRTHRDPP PQQLYGGLGQSPPGRPPIPTDQPLNTKPPPEWG PH*P*RMHRLE*LPHPKARWGRRPPNAQG*PPY HSIWDLHCKGLFMTWSNSDSSSMTFMRGWFHPGQ TA*QAYGVPMLVSIVSLGKTFVLPCLPTCTLLL YMRGGPITAQCTAAQTMELWSSKDRRRRCIAQV Q*VLEIYKGHPANEVPLHTKEIIML*TQQLPTRS PTGLYNTECKSAIITGFSMQCRLMMAPQDPHQ* TAFRRTPGSLPGVILSCLRSFTCFSTSSHLFRQ MQRPTCSTCALVTTK*RWRCVG*GESSIWLTFWT TEFWKFRRMLVVPFETSFLASLQMKIK*Q*RML VGYLPCCDC*ENLLMQK*GSLLQEFFGIYPHVM L*K*QSFEMLSQP*QTL*LFHILDGITLLLMMII KLNFRLH*FCVTRQVA*GTSAPRGKKLGSKCGP ARGW*THCCM*STRV*THPITTARRWRTACAP* GTCPIGWSWRCPRPGYWD*TNWMTY*EKSLPAKT LSQVAGGRRRKRKRGLRKKINGMELVLSQDCRS PPKGLRCCGTHRW*NHI*LF*QKVPTQPPWKAL QGLSRTSLLATGSLQHISGRPSEKKRGSPSLWSF *EWITIELFLPWQQP*GIWH*MFATRSS*ANTP CETWSTGSPAAMAPVSCLMRPWQPSAVLCTRSP AKTWRTQKPWPTQEA*RSW*T*PKAGATDHL*KW *RQQPRS*IHYGNIGTSGAFIKRMGGIRTILLH LCRHWSETDSNHILPCLPPTNRCHPSFSQAPAN LHQFTSVPIPHQQENKIDGYSINSCIIVKMTPTE RTLMHTDCICSLLIAMKILILMTEFTFQLLLIT QHSMD*NRPQIM*TFIPLNDLLIEQNSTQGPQT HGCSIKMPNRGTLSF*PCSD*GEKSILLI**LKC ESEVEGMNEVCFFFLF*GIIREVRKCLGEDFLS SI*VLDLITYRFCSLVKVWVT**EV*EMGEMKW GICRSNQIKDDFFNVNKVMF**FVQKK*NGCPCF IAITKCQDCMLLCLVNFFCWCKYGNATLVKCHH L*CNVSLEKRFEETDNFKNK*EAQGTVSN*KQT ATPFVSTTHSVLWKHNVQAGTAAPCPFLASFIGH FTAIFYSHKRKINVEISSLE

Frame 1

PGLSPRRRRPRRRHHGQGVEGQVGHRRRRGWWE SRSGGGGRWGREGRAAAPPRHFFNFFGCRSGDP SAPMSLIPGATTAAA*AGKRNASS*AGLIGGGGA TTDPPGSCLHWPRHGTRDHSHHYSSIREGAGAS VSATHPRTGSGKADCCQSARKM*AWSRITKHRQ HQLN*EVISLEINRRAKYWCKQT*SF*RCPAQQL SHQDRARTRNPLFTRTDISP*K*GIIG*LKKFN TNEFLFRQWIPGSREFPQQPEREQGRQQTAAFI HRIN*QPCGEEFKS*RTNTGSAISSQSGHEKS*F SSI*STVSFLCYQHRRVSFKGVSENFSG*WIWL SVSDRPPTSEPQCIFLHHITCCTGSLSVLTETR LPNSYTADWVSHLPADLQSQRTNPSIPNHRQSGV PTDPDGCTDSSSFPIPRPGGVVVPQTLRDDRRT TASGTFTAKDCS*HGAIRTAAV*HL*EDGSTQA RQPDRLTEFLC*SA*SAWARPSFCRVSRLAHYSY I*GEDLLQPSVPQPKPWNCGAPRIADGVVSHRF SRYWKSTKDIQPTKYPYIPKK*LCSEHNSYLRG ALQAYTIPSARVQL*QASACSAG**WHHKIPINR QHSEGPQGVCLA*S*VA*GHSHASAPVPICSGK CSGLPAAPVLW*QQSEDGGV*VRGNQASG*PSG PQSFGSSEECLWCPSKPRFWQVYR*K*NSNEECW WDTCLVATVEKIY*CRSKGACYRSSLEFILM*C CKNDNHSRCSLNLNKHCDCSTFWME*LFF***S *N*ISDFTSSA*HDRLPKEPQLRGGRSSEANAVL RGAGRLTVVCDPHVCEHIRLRQQDGGELRVHPE EPVLSAGAGGAPGPVTGTERIG*LTRKRVSQQR L*AKLLGEEEEKEKEDSARRSMGWSWSYPRTVEV PQRG*DAVAPIGGKTISDSSSRKFQPSHLGRLC RVSPEPLCWQLEVCSIYPGGRPKRKGAPHPCGA SENG*R*SCFFRGNSLEEYGTRCSQQGAHRQIRH ARPGQPAPRRQWPQCLV**DHGSHLLCSARGHQ QKHGERKSPGRLRRHREAGEHNQRQGRQIISES GEGSSPGLEYIMAISGPPEHL*KGWVESEPFYYT CVDIGARPIQITSFLVYHQPTDVTHHSVRLQQT FTNLHQFLFLTSKRTK*TATASTAVL*SR*LQQ KEL*CIQIVFAVSS*L*RSLF**PSSLSSFY*LL NTVWTEIDHKLCRLLFH*TTFL*SRTVPRVPRL MGVASRCPTEELFLSNLVQIEVKSPSC*FDD*N VKVKWKE*MKCVFFFFFEELSGK*GNVWERTF*A LFRC*I*LLIDSVVW*RCG*RDERFEKWVK*NG GYVGQIKLKMIFLM*IKLCSDSLYRKNKMDAHV LLLLLNVKIVCYYVL*ISFVGVNMEMPHWLSAII CNAMCHLKRDLKKLTTSKTNEKPKEL*AIKSKP RHPLSPPHIVYFGSTTSRLVPQRHAHSSPHS*D TSLPFSIHIKEK*MWKFHPWK

Frame 2

RG*VRVDAGRGGGTMGKG*RGKLATAAAGGGGR AAPGAGAGGGGRGGQPRRRGTFLIFSGAAAATP RRRCP*SLERRRPLPKLERGMPAPEQASLVEEG QPQTRQEAASTGPGMEPETTATTILASVKEQELQ FQRLTRELEVERQIVASQLERCRLGAESPSIAS TSSTEKSFPWRSTDVPNTGVSKPRVSDAVQPNN YLIRTEPEQGTLYSPEQTSLHESEGSLGNSRSST QMNSYSDSGYQEAGSFHNSQNVSKADNRQQHSF IGSTNNHVVRNSRAEGQTLVQPSVANRAMRRVS SVPSRAQSPSYVISTGVSPSRGSLRTSLGSGFGS PSVTDPRPLNPSAYSSTTLPAARAASPYSQRPA SPTAIRRIGSVTSRQTSNPNGPTPQYQTTARVG SPLTLTDAQTRVASPSQGQVGSSSPKRSGMTAVP QHLGPSLQRTVHDMEQFGQQQYDIYERMVPPRP DSLTGLRSSYASQHSQLGQDLRSAVSPDLHITP IYEGRTYYSPVYRSPNHGTVELQGSQTALYRTGS VGIGNLQRTSSQRSTLTYQRNNYALNTTATYAE PYRPIQYRVQECNYNRLQHAVPADDGTTRSPSI DSIQKDPREFAWRDPELPEVIHMLQHQFPSVQAN AAAYLQHLCFGDNKVKMEVCRLGGIKHLVDLLD HRVLEVQKNACGALRNLVFGKSTDENKIAMKNV GGIPALLRLLRKSIDAEVRELVTGVLWNLSSCDA VKMTIIRDALSTLTNTVIVPHSGWNNSSFDDDH KIKFQTSLVLRNTTGCLRNLSSAGEEARKQMRS CEGLVDSLLYVIHTCVNTSDYDSKTVENCVCTLR NLSYRLELEVPQARLLGLNELDDLLGKESPSKD SEPSCWGKKKKKKKRTPQEDQWDGVGPIPGLSK SPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSA GSLQNLSAGNWKFAAYIRAAVRKEKGLPILVEL LRMDNDRVVSSVATALRNMALDVRNKELIGKYA MRDLVNRLPGGNGPSVLSDETMAAICCALHEVTS KNMENAKALADSGGIEKLVNITKGRGDRSSLKV VKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFIT PVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKP SPIYISSYSSPAREQNRRLQHQQLYYSQDDSNR KNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDY STQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDS WV*HQDAQQRNSFFLTLFRLR*KVHLADLMIEM *K*SGRNE*SVFFFSFLRNYQGSEEMFGRGLSK LYLGVRSNYL*IL*SGEGVGDVMRGLRNG*NEMG DM*VKSN*R*FF*CE*SYVLIVCTEKIKWMPMF YCYY*MSRLYAIMSCKFLLLV*IWKCHIG*VPS FVMQCVT*KEI*RN*QLQKQMRSPRNCEQLKANR DTLCLHHT*CTLEAQRPGWYRSAMPIPRLIHRT LHCHFLFT*KKNKCGNFILG

Sequence NM_003628

TCCGGGGCTGAGTCCGCGTCGACGCCGGCCGCGGAGGCGGCACCATGGGCAAGGGGTAGAGGGGCAAGTTGGCCACCGCCGCCGCCGGGGGTGGTGGGAG AGCCGCTCCGGGGGCGGGGGCCGGTGGGGGAGGGAGGGGCGGGCAGCCGCGCCGCCGCGGCACTTTTTTAATTTTTTCGGGTGCCGCAGCGGCGACCCCT CGGCGCCGATGTCCCTGATCCCTGGAGCGACGACGGCCGCTGCCTAAGCTGGAAAGAGGAATGCCAGCTCCTGAGCAGGCCTCATTGGTGGAGGAGGGGC AACCACAGACCCGCCAGGAAGCTGCCTCCACTGGCCCAGGCATGGAACCCGAGACCACAGCCACCACTATTCTAGCATCCGTGAAGGAGCAGGAGCTTCA GTTTCAGCGACTCACCCGAGAACTGGAAGTGGAAAGGCAGATTGTTGCCAGTCAGCTAGAAAGATGTAGGCTTGGAGCAGAATCACCAAGCATCGCCAGC ACCAGCTCAACTGAGAAGTCATTTCCTTGGAGATCAACAGACGTGCCAAATACTGGTGTAAGCAAACCTAGAGTTTCTGACGCTGTCCAGCCCAACAACT ATCTCATCAGGACAGAGCCAGAACAAGGAACCCTCTATTCACCAGAACAGACATCTCTCCATGAAAGTGAGGGATCATTGGGTAACTCAAGAAGTTCAAC ACAAATGAATTCTTATTCCGACAGTGGATACCAGGAAGCAGGGAGTTTCCACAACAGCCAGAACGTGAGCAAGGCAGACAACAGACAGCAGCATTCATTC ATAGGATCAACTAACAACCATGTGGTGAGGAATTCAAGAGCTGAAGGACAAACACTGGTTCAGCCATCAGTAGCCAATCGGGCCATGAGAAGAGTTAGTT CAGTTCCATCTAGAGCACAGTCTCCTTCTTATGTTATCAGCACAGGCGTGTCTCCTTCAAGGGGGTCTCTGAGAACTTCTCTGGGTAGTGGATTTGGCTC TCCGTCAGTGACCGACCCCCGACCTCTGAACCCCAGTGCATATTCCTCCACCACATTACCTGCTGCACGGGCAGCCTCTCCGTACTCACAGAGACCCGCC TCCCCAACAGCTATACGGCGGATTGGGTCAGTCACCTCCCGGCAGACCTCCAATCCCAACGGACCAACCCCTCAATACCAAACCACCGCCAGAGTGGGGT CCCCACTGACCCTGACGGATGCACAGACTCGAGTAGCTTCCCCATCCCAAGGCCAGGTGGGGTCGTCGTCCCCCAAACGCTCAGGGATGACCGCCGTACC ACAGCATCTGGGACCTTCACTGCAAAGGACTGTTCATGACATGGAGCAATTCGGACAGCAGCAGTATGACATTTATGAGAGGATGGTTCCACCCAGGCCA GACAGCCTGACAGGCTTACGGAGTTCCTATGCTAGTCAGCATAGTCAGCTTGGGCAAGACCTTCGTTCTGCCGTGTCTCCCGACTTGCACATTACTCCTA TATATGAGGGGAGGACCTATTACAGCCCAGTGTACCGCAGCCCAAACCATGGAACTGTGGAGCTCCAAGGATCGCAGACGGCGTTGTATCGCACAGGTTC AGTAGGTATTGGAAATCTACAAAGGACATCCAGCCAACGAAGTACCCTTACATACCAAAGAAATAATTATGCTCTGAACACAACAGCTACCTACGCGGAG CCCTACAGGCCTATACAATACCGAGTGCAAGAGTGCAATTATAACAGGCTTCAGCATGCAGTGCCGGCTGATGATGGCACCACAAGATCCCCATCAATAG ACAGCATTCAGAAGGACCCCAGGGAGTTTGCCTGGCGTGATCCTGAGTTGCCTGAGGTCATTCACATGCTTCAGCACCAGTTCCCATCTGTTCAGGCAAA TGCAGCGGCCTACCTGCAGCACCTGTGCTTTGGTGACAACAAAGTGAAGATGGAGGTGTGTAGGTTAGGGGGAATCAAGCATCTGGTTGACCTTCTGGAC CACAGAGTTTTGGAAGTTCAGAAGAATGCTTGTGGTGCCCTTCGAAACCTCGTTTTTGGCAAGTCTACAGATGAAAATAAAATAGCAATGAAGAATGTTG GTGGGATACCTGCCTTGTTGCGACTGTTGAGAAAATCTATTGATGCAGAAGTAAGGGAGCTTGTTACAGGAGTTCTTTGGAATTTATCCTCATGTGATGC TGTAAAAATGACAATCATTCGAGATGCTCTCTCAACCTTAACAAACACTGTGATTGTTCCACATTCTGGATGGAATAACTCTTCTTTTGATGATGATCAT AAAATTAAATTTCAGACTTCACTAGTTCTGCGTAACACGACAGGTTGCCTAAGGAACCTCAGCTCCGCGGGGGAAGAAGCTCGGAAGCAAATGCGGTCCT GCGAGGGGCTGGTAGACTCACTGTTGTATGTGATCCACACGTGTGTGAACACATCCGATTACGACAGCAAGACGGTGGAGAACTGCGTGTGCACCCTGAG GAACCTGTCCTATCGGCTGGAGCTGGAGGTGCCCCAGGCCCGGTTACTGGGACTGAACGAATTGGATGACTTACTAGGAAAAGAGTCTCCCAGCAAAGAC TCTGAGCCAAGTTGCTGGGGGAAGAAGAAGAAAAAGAAAAAGAGGACTCCGCAAGAAGATCAATGGGATGGAGTTGGTCCTATCCCAGGACTGTCGAAGT CCCCCAAAGGGGTTGAGATGCTGTGGCACCCATCGGTGGTAAAACCATATCTGACTCTTCTAGCAGAAAGTTCCAACCCAGCCACCTTGGAAGGCTCTGC AGGGTCTCTCCAGAACCTCTCTGCTGGCAACTGGAAGTTTGCAGCATATATCCGGGCGGCCGTCCGAAAAGAAAAGGGGCTCCCCATCCTTGTGGAGCTT CTGAGAATGGATAACGATAGAGTTGTTTCTTCCGTGGCAACAGCCTTGAGGAATATGGCACTAGATGTTCGCAACAAGGAGCTCATAGGCAAATACGCCA TGCGAGACCTGGTCAACCGGCTCCCCGGCGGCAATGGCCCCAGTGTCTTGTCTGATGAGACCATGGCAGCCATCTGCTGTGCTCTGCACGAGGTCACCAG CAAAAACATGGAGAACGCAAAAGCCCTGGCCGACTCAGGAGGCATAGAGAAGCTGGTGAACATAACCAAAGGCAGGGGCGACAGATCATCTCTGAAAGTG GTGAAGGCAGCAGCCCAGGTCTTGAATACATTATGGCAATATCGGGACCTCCGGAGCATTTATAAAAAGGATGGGTGGAATCAGAACCATTTTATTACAC CTGTGTCGACATTGGAGCGAGACCGATTCAAATCACATCCTTCCTTGTCTACCACCAACCAACAGATGTCACCCATCATTCAGTCAGTCGGCAGCACCTC TTCCTCACCAGCACTGTTAGGAATCAGAGACCCTCGCTCTGAATACGATAGGACCCAGCCACCTATGCAGTATTACAATAGCCAAGGGGATGCCACACAT AAAGGCCTGTACCCTGGCTCCAGCAAACCTTCACCAATTTACATCAGTTCCTATTCCTCACCAGCAAGAGAACAAAATAGACGGCTACAGCATCAACAGC TGTATTATAGTCAAGATGACTCCAACAGAAAGAACTTTGATGCATACAGATTGTATTTGCAGTCTCCTCATAGCTATGAAGATCCTTATTTTGATGACCG AGTTCACTTTCCAGCTTCTACTGATTACTCAACACAGTATGGACTGAAATCGACCACAAATTATGTAGACTTTTATTCCACTAAACGACCTTCTTATAGA GCAGAACAGTACCCAGGGTCCCCAGACTCATGGGTGTAGCATCAAGATGCCCAACAGAGGAACTCTTTCTTTCTAACCTTGTTCAGATTGAGGTGAAAAG TCCATCTTGCTGATTTGATGATTGAAATGTGAAAGTGAAGTGGAAGGAATGAATGAAGTGTGTTTTTTTTTTCTTTTTTGAGGAATTATCAGGGAAGTGA GGAAATGTTTGGGAGAGGACTTTCTAAGCTCTATTTAGGTGTTAGATCTAATTACTTATAGATTCTGTAGTCTGGTGAAGGTGTGGGTGACGTGATGAGA GGTTTGAGAAATGGGTGAAATGAAATGGGGGATATGTAGGTCAAATCAAATTAAAGATGATTTTTTTAATGTGAATAAAGTTATGTTCTGATAGTTTGTA CAGAAAAAATAAAATGGATGCCCATGTTTTATTGCTATTACTAAATGTCAAGATTGTATGCTATTATGTCTTGTAAATTTCTTTTGTTGGTGTAAATATG GAAATGCCACATTGGTTAAGTGCCATCATTTGTAATGCAATGTGTCACTTGAAAAGAGATTTGAAGAAACTGACAACTTCAAAAACAAATGAGAAGCCCA AGGAACTGTGAGCAATTAAAAGCAAACCGCGACACCCTTTGTCTCCACCACACATAGTGTACTTTGGAAGCACAACGTCCAGGCTGGTACCGCAGCGCCA TGCCCATTCCTCGCCTCATTCATAGGACACTTCACTGCCATTTTCTATTCACATAAAAGAAAAATAAATGTGGAAATTTCATCCTTGGAAA

refseq_PKP4.F2 (forward primer)
refseq_PKP4.R2 (reverse primer)
Single exon skipping

Frame 0

SGAESASTPAAEAAPWARGRGASWPPPPPGVVG EPLRGRGPVGEGGAGSRAAAALF*FFRVPQRRPL GADVPDPWSDDGRCLSWKEECQLLSRPHWWRRG NHRPARKLPPLAQAWNPRPQPPLF*HP*RSRSF SFSDSPENWKWKGRLLPVS*KDVGLEQNHQASPA PAQLRSHFLGDQQTCQILV*ANLEFLTLSSPTT ISSGQSQNKEPSIHQNRHLSMKVRDHWVTQEVQ HK*ILIPTVDTRKQGVSTTART*ARQTTDSSIHS *DQLTTMW*GIQELKDKHWFSHQ*PIGP*EELV QFHLEHSLLLMLSAQACLLQGGL*ELLWVVDLA LRQ*PTPDL*TPVHIPPPHYLLHGQPLRTHRDPP PQQLYGGLGQSPPGRPPIPTDQPLNTKPPPEWG PH*P*RMHRLE*LPHPKARWGRRPPNAQG*PPY HSIWDLHCKGLFMTWSNSDSSSMTFMRGWFHPGQ TA*QAYGVPMLVSIVSLGKTFVLPCLPTCTLLL YMRGGPITAQCTAAQTMELWSSKDRRRRCIAQV Q*VLEIYKGHPANEVPLHTKEIIML*TQQLPTRS PTGLYNTECKSAIITGFSMQCRLMMAPQDPHQ* TAFRRTPGSLPGVILSCLRSFTCFSTSSHLFRQ MQRPTCSTCALVTTK*RWRCVG*GESSIWLTFWT TEFWKFRRMLVVPFETSFLASLQMKIK*Q*RML VGYLPCCDC*ENLLMQK*GSLLQEFFGIYPHVM L*K*QSFEMLSQP*QTL*LFHILDGITLLLMMII KLNFRLH*FCVTRQVA*GTSAPRGKKLGSKCGP ARGW*THCCM*STRV*THPITTARRWRTACAP* GTCPIGWSWRCPRPGYWD*TNWMTY*EKSLPAKT LSQVAGGRRRKRKRGLRKKINGMELVLSQDCRS PPKGLRCCGTHRW*NHI*LF*QKVPTQPPWKAL QGLSRTSLLATGSLQHISGRPSEKKRGSPSLWSF *EWITIELFLPWQQP*GIWH*MFATRSS*ANTP CETWSTGSPAAMAPVSCLMRPWQPSAVLCTRSP AKTWRTQKPWPTQEA*RSW*T*PKAGATDHL*KW *RQQPRS*IHYGNIGTSGAFIKRMGGIRTILLH LCRHWSETDSNHILPCLPPTNRCHPSFSQSAAP LPHQHC*ESETLALNTIGPSHLCSITIAKGMPHI KACTLAPANLHQFTSVPIPHQQENKIDGYSINS CIIVKMTPTERTLMHTDCICSLLIAMKILILMT EFTFQLLLITQHSMD*NRPQIM*TFIPLNDLLIE QNSTQGPQTHGCSIKMPNRGTLSF*PCSD*GEK SILLI**LKCESEVEGMNEVCFFFLF*GIIREV RKCLGEDFLSSI*VLDLITYRFCSLVKVWVT**E V*EMGEMKWGICRSNQIKDDFFNVNKVMF**FV QKK*NGCPCFIAITKCQDCMLLCLVNFFCWCKY GNATLVKCHHL*CNVSLEKRFEETDNFKNK*EAQ GTVSN*KQTATPFVSTTHSVLWKHNVQAGTAAP CPFLASFIGHFTAIFYSHKRKINVEISSLE

Frame 1

PGLSPRRRRPRRRHHGQGVEGQVGHRRRRGWWE SRSGGGGRWGREGRAAAPPRHFFNFFGCRSGDP SAPMSLIPGATTAAA*AGKRNASS*AGLIGGGGA TTDPPGSCLHWPRHGTRDHSHHYSSIREGAGAS VSATHPRTGSGKADCCQSARKM*AWSRITKHRQ HQLN*EVISLEINRRAKYWCKQT*SF*RCPAQQL SHQDRARTRNPLFTRTDISP*K*GIIG*LKKFN TNEFLFRQWIPGSREFPQQPEREQGRQQTAAFI HRIN*QPCGEEFKS*RTNTGSAISSQSGHEKS*F SSI*STVSFLCYQHRRVSFKGVSENFSG*WIWL SVSDRPPTSEPQCIFLHHITCCTGSLSVLTETR LPNSYTADWVSHLPADLQSQRTNPSIPNHRQSGV PTDPDGCTDSSSFPIPRPGGVVVPQTLRDDRRT TASGTFTAKDCS*HGAIRTAAV*HL*EDGSTQA RQPDRLTEFLC*SA*SAWARPSFCRVSRLAHYSY I*GEDLLQPSVPQPKPWNCGAPRIADGVVSHRF SRYWKSTKDIQPTKYPYIPKK*LCSEHNSYLRG ALQAYTIPSARVQL*QASACSAG**WHHKIPINR QHSEGPQGVCLA*S*VA*GHSHASAPVPICSGK CSGLPAAPVLW*QQSEDGGV*VRGNQASG*PSG PQSFGSSEECLWCPSKPRFWQVYR*K*NSNEECW WDTCLVATVEKIY*CRSKGACYRSSLEFILM*C CKNDNHSRCSLNLNKHCDCSTFWME*LFF***S *N*ISDFTSSA*HDRLPKEPQLRGGRSSEANAVL RGAGRLTVVCDPHVCEHIRLRQQDGGELRVHPE EPVLSAGAGGAPGPVTGTERIG*LTRKRVSQQR L*AKLLGEEEEKEKEDSARRSMGWSWSYPRTVEV PQRG*DAVAPIGGKTISDSSSRKFQPSHLGRLC RVSPEPLCWQLEVCSIYPGGRPKRKGAPHPCGA SENG*R*SCFFRGNSLEEYGTRCSQQGAHRQIRH ARPGQPAPRRQWPQCLV**DHGSHLLCSARGHQ QKHGERKSPGRLRRHREAGEHNQRQGRQIISES GEGSSPGLEYIMAISGPPEHL*KGWVESEPFYYT CVDIGARPIQITSFLVYHQPTDVTHHSVSRQHL FLTSTVRNQRPSL*IR*DPATYAVLQ*PRGCHT *RPVPWLQQTFTNLHQFLFLTSKRTK*TATASTA VL*SR*LQQKEL*CIQIVFAVSS*L*RSLF**P SSLSSFY*LLNTVWTEIDHKLCRLLFH*TTFL* SRTVPRVPRLMGVASRCPTEELFLSNLVQIEVKS PSC*FDD*NVKVKWKE*MKCVFFFFFEELSGK* GNVWERTF*ALFRC*I*LLIDSVVW*RCG*RDE RFEKWVK*NGGYVGQIKLKMIFLM*IKLCSDSLY RKNKMDAHVLLLLLNVKIVCYYVL*ISFVGVNM EMPHWLSAIICNAMCHLKRDLKKLTTSKTNEKP KEL*AIKSKPRHPLSPPHIVYFGSTTSRLVPQRH AHSSPHS*DTSLPFSIHIKEK*MWKFHPWK

Frame 2

RG*VRVDAGRGGGTMGKG*RGKLATAAAGGGGR AAPGAGAGGGGRGGQPRRRGTFLIFSGAAAATP RRRCP*SLERRRPLPKLERGMPAPEQASLVEEG QPQTRQEAASTGPGMEPETTATTILASVKEQELQ FQRLTRELEVERQIVASQLERCRLGAESPSIAS TSSTEKSFPWRSTDVPNTGVSKPRVSDAVQPNN YLIRTEPEQGTLYSPEQTSLHESEGSLGNSRSST QMNSYSDSGYQEAGSFHNSQNVSKADNRQQHSF IGSTNNHVVRNSRAEGQTLVQPSVANRAMRRVS SVPSRAQSPSYVISTGVSPSRGSLRTSLGSGFGS PSVTDPRPLNPSAYSSTTLPAARAASPYSQRPA SPTAIRRIGSVTSRQTSNPNGPTPQYQTTARVG SPLTLTDAQTRVASPSQGQVGSSSPKRSGMTAVP QHLGPSLQRTVHDMEQFGQQQYDIYERMVPPRP DSLTGLRSSYASQHSQLGQDLRSAVSPDLHITP IYEGRTYYSPVYRSPNHGTVELQGSQTALYRTGS VGIGNLQRTSSQRSTLTYQRNNYALNTTATYAE PYRPIQYRVQECNYNRLQHAVPADDGTTRSPSI DSIQKDPREFAWRDPELPEVIHMLQHQFPSVQAN AAAYLQHLCFGDNKVKMEVCRLGGIKHLVDLLD HRVLEVQKNACGALRNLVFGKSTDENKIAMKNV GGIPALLRLLRKSIDAEVRELVTGVLWNLSSCDA VKMTIIRDALSTLTNTVIVPHSGWNNSSFDDDH KIKFQTSLVLRNTTGCLRNLSSAGEEARKQMRS CEGLVDSLLYVIHTCVNTSDYDSKTVENCVCTLR NLSYRLELEVPQARLLGLNELDDLLGKESPSKD SEPSCWGKKKKKKKRTPQEDQWDGVGPIPGLSK SPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSA GSLQNLSAGNWKFAAYIRAAVRKEKGLPILVEL LRMDNDRVVSSVATALRNMALDVRNKELIGKYA MRDLVNRLPGGNGPSVLSDETMAAICCALHEVTS KNMENAKALADSGGIEKLVNITKGRGDRSSLKV VKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFIT PVSTLERDRFKSHPSLSTTNQQMSPIIQSVGSTS SSPALLGIRDPRSEYDRTQPPMQYYNSQGDATH KGLYPGSSKPSPIYISSYSSPAREQNRRLQHQQ LYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDR VHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYR AEQYPGSPDSWV*HQDAQQRNSFFLTLFRLR*K VHLADLMIEM*K*SGRNE*SVFFFSFLRNYQGSE EMFGRGLSKLYLGVRSNYL*IL*SGEGVGDVMR GLRNG*NEMGDM*VKSN*R*FF*CE*SYVLIVC TEKIKWMPMFYCYY*MSRLYAIMSCKFLLLV*IW KCHIG*VPSFVMQCVT*KEI*RN*QLQKQMRSP RNCEQLKANRDTLCLHHT*CTLEAQRPGWYRSA MPIPRLIHRTLHCHFLFT*KKNKCGNFILG