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Sequences

Sequence NM_182471

CGCCGCGCTTCCTCCTGAAGGTGACTGCGCCCGCGGGGACGCAGGGGGCGGGGCCCGGGTCGCCCGGAGCCGGGATTGGGCAGAGGGCGGGGCGGCGGAG GGATTGCGGCGGCCCGCAGCGGGATAACCTTGAGGCTGAGGCAGTGGCTCCTTGCACAGCAGCTGCACGCGCCGTGGCTCCGGATCTCTTCGTCTTTGCA GCGTAGCCCGAGTCGGTCAGCGCCGGAGGACCTCAGCAGCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGACCCAGCAGCTGCACGC AGCCATGGCTGACACATTCCTGGAGCACATGTGCCGCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATCTGTACCATTGGCCCA GCTTCCCGATCAGTGGAGACGTTGAAGGAGATGATTAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCATGAGTACCATGCGGAGA CCATCAAGAATGTGCGCACAGCCACGGAAAGCTTTGCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGCTCTAGACACTAAAGGACCTGAGATCCG AACTGGGCTCATCAAGGGCAGCGGCACTGCAGAGGTGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTACATGGAAAAGTGTGAC GAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTTCTCTCCAGGTGAAGC AGAAAGGTGCCGACTTCCTGGTGACGGAGGTGGAAAATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTGCTGTGGACTTGCCTGC TGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTGGGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCATCTGATGTCCATGAA GTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACATCAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGATGAAATCCTGGAGGCCA GTGATGGGATCATGGTGGCTCGTGGTGATCTAGGCATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTGGACGGTGCAACCGAGC TGGGAAGCCTGTCATCTGTGCTACTCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTGGCCAATGCAGTCCTG GATGGAGCCGACTGCATCATGCTGTCTGGAGAAACAGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATAGCTCGTGAGGCTGAGG CAGCCATGTTCCACCGCAAGCTGTTTGAAGAACTTGTGCGAGCCTCAAGTCACTCCACAGACCTCATGGAAGCCATGGCCATGGGCAGCGTGGAGGCTTC TTATAAGTGTTTAGCAGCAGCTTTGATAGTTCTGACGGAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCCCCCATCATTGCTGTG ACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCTGGGCTGAGGACGTGG ACCTCCGGGTGAACTTTGCCATGAATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTGGCTCCGG CTTCACCAACACCATGCGTGTTGTTCCTGTGCCGTGATGGACCCCAGAGCCCCTCCTCCAGCCCCTGTCCCACCCCCTTCCCCCAGCCCATCCATTAGGC CAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAACGCCTCACTGAAACATGGCTGTGTTT GCAGCCTGCTCTAGTGGGACAGCCCAGAGCCTGGCTGCCCATCATGTGGCCCCACCCAATCAAGGGAAGAAGGAGGAATGCTGGACTGGAGGCCCCTGGA GCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTCTGTCCAGTTCCTTTAGAAAAAATGGATGCCCAGAGGACTCCCAACCCTGGCTTGGG GTCAAGAAACAGCCAGCAAGAGTTAGGGGCCTTAGGGCACTGGGCTGTTGTTCCATTGAAGCCGACTCTGGCCCTGGCCCTTACTTGCTTCTCTAGCTCT CTAGGCCTCTCCAGTTTGCACCTGTCCCCACCCTCCACTCAGCTGTCCTGCAGCAAACACTCCACCCTCCACCTTCCATTTTCCCCCACTACTGCAGCAC CTCCAGGCCTGTTGCTATAGAGCCTACCTGTATGTCAATAAACAACAGCTGAAGCACC

refseq_PKM2.F1 (forward primer)
refseq_PKM2.R1 (reverse primer)
Alternative donor

Frame 0

RRASS*R*LRPRGRRGRGPGRPEPGLGRGRGGG GIAAARSGITLRLRQWLLAQQLHAPWLRISSSLQ RSPSRSAPEDLSSHVEAP**SRDCLHSDPAAAR SHG*HIPGAHVPPGH*FTTHHSPEHWHHLYHWP SFPISGDVEGDD*VWNECGSSELLSWNS*VPCGD HQECAHSHGKLCF*PHPLPARCCGSRH*RT*DP NWAHQGQRHCRGGAEEGSHSQNHAG*RLHGKV* REHPVAGLQEHLQGGGSGQQDLRG*WAYFSPGEA ERCRLPGDGGGKWWLLGQQEGCEPSWGCCGLAC CVGEGHPGSEVWGRAGC*YGVCVIHPQGI*CP* S*EGPGREGKEHQDYQQNRES*GGSEV**NPGGQ *WDHGGSW*SRH*DSCREGLPCSEDDDWTVQPS WEACHLCYSDAGEHDQEAPPHSG*RQ*CGQCSP GWSRLHHAVWRNSQRGLSSGGCAHAAPDSS*G*G SHVPPQAV*RTCASLKSLHRPHGSHGHGQRGGF L*VFSSSFDSSDGVWQVCSPGGQIPPTCPHHCC DPESPDSSSGPPVPWHLPCAVQGPSPGGLG*GRG PPGELCHECWQGPRLLQEGRCGHCADRMAPWLR LHQHHACCSCAVMDPRAPPPAPVPPPSPSPSIR PATLVELTLGCNVALVGWDTREEDQRLTETWLCL QPALVGQPRAWLPIMWPHPIKGRRRNAGLEAPG ARWQEGDSFLSCVYSVQFL*KKWMPRGLPTLAW GQETASKS*GP*GTGLLFH*SRLWPWPLLASLAL *ASPVCTCPHPPLSCPAANTPPSTFHFPPLLQH LQACCYRAYLYVNKQQLKH

Frame 1

AALPPEGDCARGDAGGGARVARSRDWAEGGAAE GLRRPAAG*P*G*GSGSLHSSCTRRGSGSLRLC SVARVGQRRRTSAAMSKPHSEAGTAFIQTQQLHA AMADTFLEHMCRLDIDSPPITARNTGIICTIGP ASRSVETLKEMIKSGMNVARLNFSHGTHEYHAE TIKNVRTATESFASDPILYRPVAVALDTKGPEIR TGLIKGSGTAEVELKKGATLKITLDNAYMEKCD ENILWLDYKNICKVVEVGSKIYVDDGLISLQVK QKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPA VSEKDIQDLKFGVEQDVDMVFASFIRKASDVHE VRKVLGEKGKNIKIISKIENHEGVRRFDEILEA SDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRA GKPVICATQMLESMIKKPRPTRAEGSDVANAVL DGADCIMLSGETAKGDYPLEAVRMQHLIAREAE AAMFHRKLFEELVRASSHSTDLMEAMAMGSVEAS YKCLAAALIVLTESGRSAHQVARYRPRAPIIAV TRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDV DLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSG FTNTMRVVPVP*WTPEPLLQPLSHPLPPAHPLG QQRL*NSLWAVTWHW*VGTPGKKINASLKHGCV CSLL*WDSPEPGCPSCGPTQSREEGGMLDWRPLE PDGKRVTASFPVCTLSSSFRKNGCPEDSQPWLG VKKQPARVRGLRALGCCSIEADSGPGPYLLL*L SRPLQFAPVPTLHSAVLQQTLHPPPSIFPHYCST SRPVAIEPTCMSINNS*ST

Frame 2

PRFLLKVTAPAGTQGAGPGSPGAGIGQRAGRRR DCGGPQRDNLEAEAVAPCTAAARAVAPDLFVFA A*PESVSAGGPQQPCRSPIVKPGLPSFRPSSCT QPWLTHSWSTCAAWTLIHHPSQPGTLASSVPLAQ LPDQWRR*RR*LSLE*MWLV*TSLMELMSTMRR PSRMCAQPRKALLLTPSSTGPLLWL*TLKDLRS ELGSSRAAALQRWS*RREPLSKSRWITPTWKSVT RTSCGWTTRTSARWWKWAARSTWMMGLFLSR*S RKVPTSW*RRWKMVAPWAARRV*TFLGLLWTCL LCRRRTSRI*SLGSSRMLIWCLRHSSARHLMSMK LGRSWERRERTSRLSAKSRIMRGFGGLMKSWRP VMGSWWLVVI*ALRFLQRRSSLLRR**LDGATE LGSLSSVLLRCWRA*SRSPAPLGLKAVMWPMQSW MEPTASCCLEKQPKGTILWRLCACST**LVRLR QPCSTASCLKNLCEPQVTPQTSWKPWPWAAWRL LISV*QQL**F*RSLAGLLTRWPDTAHVPPSLL* PGIPRQLVRPTCTVASSLCCARTQSRRPGLRTW TSG*TLP*MLARPEASSRREMWSLC*PDGALAP ASPTPCVLFLCRDGPQSPSSSPCPTPFPQPIH*A SNACRTHSGL*RGTGRLGHQGRRSTPH*NMAVF AACSSGTAQSLAAHHVAPPNQGKKEECWTGGPW SQMARG*QLPFLCVLCPVPLEKMDAQRTPNPGLG SRNSQQELGALGHWAVVPLKPTLALALTCFSSS LGLSSLHLSPPSTQLSCSKHSTLHLPFSPTTAA PPGLLL*SLPVCQ*TTAEA

Sequence NM_002654

CGCCGCGCTTCCTCCTGAAGGTGACTGCGCCCGCGGGGACGCAGGGGGCGGGGCCCGGGTCGCCCGGAGCCGGGATTGGGCAGAGGGCGGGGCGGCGGAG GGATTGCGGCGGCCCGCAGCGGGATAACCTTGAGGCTGAGGCAGTGGCTCCTTGCACAGCAGCTGCACGCGCCGTGGCTCCGGATCTCTTCGTCTTTGCA GCGTAGCCCGAGTCGGTCAGCGCCGGAGGACCTCAGCAGCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGACCCAGCAGCTGCACGC AGCCATGGCTGACACATTCCTGGAGCACATGTGCCGCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCATCTGTACCATTGGCCCA GCTTCCCGATCAGTGGAGACGTTGAAGGAGATGATTAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCATGAGTACCATGCGGAGA CCATCAAGAATGTGCGCACAGCCACGGAAAGCTTTGCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGCTCTAGACACTAAAGGACCTGAGATCCG AACTGGGCTCATCAAGGGCAGCGGCACTGCAGAGGTGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTACATGGAAAAGTGTGAC GAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATTTCTCTCCAGGTGAAGC AGAAAGGTGCCGACTTCCTGGTGACGGAGGTGGAAAATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTGCTGTGGACTTGCCTGC TGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTGGGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGCATCTGATGTCCATGAA GTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACATCAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGATGAAATCCTGGAGGCCA GTGATGGGATCATGGTGGCTCGTGGTGATCTAGGCATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTGGACGGTGCAACCGAGC TGGGAAGCCTGTCATCTGTGCTACTCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGTGGCCAATGCAGTCCTG GATGGAGCCGACTGCATCATGCTGTCTGGAGAAACAGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATTGCCCGTGAGGCAGAGG CTGCCATCTACCACTTGCAATTATTTGAGGAACTCCGCCGCCTGGCGCCCATTACCAGCGACCCCACAGAAGCCACCGCCGTGGGTGCCGTGGAGGCCTC CTTCAAGTGCTGCAGTGGGGCCATAATCGTCCTCACCAAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGCCCCCATCATTGCTGTG ACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCCTGGGCTGAGGACGTGG ACCTCCGGGTGAACTTTGCCATGAATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGATGGCGCCCTGGCTCCGG CTTCACCAACACCATGCGTGTTGTTCCTGTGCCGTGATGGACCCCAGAGCCCCTCCTCCAGCCCCTGTCCCACCCCCTTCCCCCAGCCCATCCATTAGGC CAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAACGCCTCACTGAAACATGGCTGTGTTT GCAGCCTGCTCTAGTGGGACAGCCCAGAGCCTGGCTGCCCATCATGTGGCCCCACCCAATCAAGGGAAGAAGGAGGAATGCTGGACTGGAGGCCCCTGGA GCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTCTGTCCAGTTCCTTTAGAAAAAATGGATGCCCAGAGGACTCCCAACCCTGGCTTGGG GTCAAGAAACAGCCAGCAAGAGTTAGGGGCCTTAGGGCACTGGGCTGTTGTTCCATTGAAGCCGACTCTGGCCCTGGCCCTTACTTGCTTCTCTAGCTCT CTAGGCCTCTCCAGTTTGCACCTGTCCCCACCCTCCACTCAGCTGTCCTGCAGCAAACACTCCACCCTCCACCTTCCATTTTCCCCCACTACTGCAGCAC CTCCAGGCCTGTTGCTATAGAGCCTACCTGTATGTCAATAAACAACAGCTGAAGCACC

refseq_PKM2.F1 (forward primer)
refseq_PKM2.R1 (reverse primer)
Alternative donor

Frame 0

RRASS*R*LRPRGRRGRGPGRPEPGLGRGRGGG GIAAARSGITLRLRQWLLAQQLHAPWLRISSSLQ RSPSRSAPEDLSSHVEAP**SRDCLHSDPAAAR SHG*HIPGAHVPPGH*FTTHHSPEHWHHLYHWP SFPISGDVEGDD*VWNECGSSELLSWNS*VPCGD HQECAHSHGKLCF*PHPLPARCCGSRH*RT*DP NWAHQGQRHCRGGAEEGSHSQNHAG*RLHGKV* REHPVAGLQEHLQGGGSGQQDLRG*WAYFSPGEA ERCRLPGDGGGKWWLLGQQEGCEPSWGCCGLAC CVGEGHPGSEVWGRAGC*YGVCVIHPQGI*CP* S*EGPGREGKEHQDYQQNRES*GGSEV**NPGGQ *WDHGGSW*SRH*DSCREGLPCSEDDDWTVQPS WEACHLCYSDAGEHDQEAPPHSG*RQ*CGQCSP GWSRLHHAVWRNSQRGLSSGGCAHAAPDCP*GRG CHLPLAII*GTPPPGAHYQRPHRSHRRGCRGGL LQVLQWGHNRPHQVWQVCSPGGQIPPTCPHHCC DPESPDSSSGPPVPWHLPCAVQGPSPGGLG*GRG PPGELCHECWQGPRLLQEGRCGHCADRMAPWLR LHQHHACCSCAVMDPRAPPPAPVPPPSPSPSIR PATLVELTLGCNVALVGWDTREEDQRLTETWLCL QPALVGQPRAWLPIMWPHPIKGRRRNAGLEAPG ARWQEGDSFLSCVYSVQFL*KKWMPRGLPTLAW GQETASKS*GP*GTGLLFH*SRLWPWPLLASLAL *ASPVCTCPHPPLSCPAANTPPSTFHFPPLLQH LQACCYRAYLYVNKQQLKH

Frame 1

AALPPEGDCARGDAGGGARVARSRDWAEGGAAE GLRRPAAG*P*G*GSGSLHSSCTRRGSGSLRLC SVARVGQRRRTSAAMSKPHSEAGTAFIQTQQLHA AMADTFLEHMCRLDIDSPPITARNTGIICTIGP ASRSVETLKEMIKSGMNVARLNFSHGTHEYHAE TIKNVRTATESFASDPILYRPVAVALDTKGPEIR TGLIKGSGTAEVELKKGATLKITLDNAYMEKCD ENILWLDYKNICKVVEVGSKIYVDDGLISLQVK QKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPA VSEKDIQDLKFGVEQDVDMVFASFIRKASDVHE VRKVLGEKGKNIKIISKIENHEGVRRFDEILEA SDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRA GKPVICATQMLESMIKKPRPTRAEGSDVANAVL DGADCIMLSGETAKGDYPLEAVRMQHLIAREAE AAIYHLQLFEELRRLAPITSDPTEATAVGAVEAS FKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAV TRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDV DLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSG FTNTMRVVPVP*WTPEPLLQPLSHPLPPAHPLG QQRL*NSLWAVTWHW*VGTPGKKINASLKHGCV CSLL*WDSPEPGCPSCGPTQSREEGGMLDWRPLE PDGKRVTASFPVCTLSSSFRKNGCPEDSQPWLG VKKQPARVRGLRALGCCSIEADSGPGPYLLL*L SRPLQFAPVPTLHSAVLQQTLHPPPSIFPHYCST SRPVAIEPTCMSINNS*ST

Frame 2

PRFLLKVTAPAGTQGAGPGSPGAGIGQRAGRRR DCGGPQRDNLEAEAVAPCTAAARAVAPDLFVFA A*PESVSAGGPQQPCRSPIVKPGLPSFRPSSCT QPWLTHSWSTCAAWTLIHHPSQPGTLASSVPLAQ LPDQWRR*RR*LSLE*MWLV*TSLMELMSTMRR PSRMCAQPRKALLLTPSSTGPLLWL*TLKDLRS ELGSSRAAALQRWS*RREPLSKSRWITPTWKSVT RTSCGWTTRTSARWWKWAARSTWMMGLFLSR*S RKVPTSW*RRWKMVAPWAARRV*TFLGLLWTCL LCRRRTSRI*SLGSSRMLIWCLRHSSARHLMSMK LGRSWERRERTSRLSAKSRIMRGFGGLMKSWRP VMGSWWLVVI*ALRFLQRRSSLLRR**LDGATE LGSLSSVLLRCWRA*SRSPAPLGLKAVMWPMQSW MEPTASCCLEKQPKGTILWRLCACST*LPVRQR LPSTTCNYLRNSAAWRPLPATPQKPPPWVPWRP PSSAAVGP*SSSPSLAGLLTRWPDTAHVPPSLL* PGIPRQLVRPTCTVASSLCCARTQSRRPGLRTW TSG*TLP*MLARPEASSRREMWSLC*PDGALAP ASPTPCVLFLCRDGPQSPSSSPCPTPFPQPIH*A SNACRTHSGL*RGTGRLGHQGRRSTPH*NMAVF AACSSGTAQSLAAHHVAPPNQGKKEECWTGGPW SQMARG*QLPFLCVLCPVPLEKMDAQRTPNPGLG SRNSQQELGALGHWAVVPLKPTLALALTCFSSS LGLSSLHLSPPSTQLSCSKHSTLHLPFSPTTAA PPGLLL*SLPVCQ*TTAEA

Sequence NM_182470

CGCCGCGCTTCCTCCTGAAGGTGACTGCGCCCGCGGGGACGCAGGGGGCGGGGCCCGGGTCGCCCGGAGCCGGGATTGGGCAGAGGGCGGGGCGGCGGAG GGATTGCGGCGGCCCGCAGCGGGATAACCTTGAGGCTGAGGCAGTGGCTCCTTGCACAGCAGCTGCACGCGCCGTGGCTCCGGATCTCTTCGTCTTTGCA GCGTAGCCCGAGTCGGTCAGCGCCGGAGGTGAGCGGTGCAGGAGGCTACGCCATCAGTCCCCACCAAGGGCCAGTCGCCCGGCTAGTGCGGAATCCCGGC GCGCCGGCCGGCCCCGGGCACGCAGGCAGGGCGGCGCAGGATCCAGGGCGTCTGGGATGCAGTGGAGCTCAGAGAGAGGAGAACGGCTCCTCACGCCTGG GGCCTGCTCTTCAGAAGTCCCCAGCGCCGTTCCTTCCAGATCAGGACCTCAGCAGCCATGTCGAAGCCCCATAGTGAAGCCGGGACTGCCTTCATTCAGA CCCAGCAGCTGCACGCAGCCATGGCTGACACATTCCTGGAGCACATGTGCCGCCTGGACATTGATTCACCACCCATCACAGCCCGGAACACTGGCATCAT CTGTACCATTGGCCCAGCTTCCCGATCAGTGGAGACGTTGAAGGAGATGATTAAGTCTGGAATGAATGTGGCTCGTCTGAACTTCTCTCATGGAACTCAT GAGTACCATGCGGAGACCATCAAGAATGTGCGCACAGCCACGGAAAGCTTTGCTTCTGACCCCATCCTCTACCGGCCCGTTGCTGTGGCTCTAGACACTA AAGGACCTGAGATCCGAACTGGGCTCATCAAGGGCAGCGGCACTGCAGAGGTGGAGCTGAAGAAGGGAGCCACTCTCAAAATCACGCTGGATAACGCCTA CATGGAAAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGGTGGAAGTGGGCAGCAAGATCTACGTGGATGATGGGCTTATT TCTCTCCAGGTGAAGCAGAAAGGTGCCGACTTCCTGGTGACGGAGGTGGAAAATGGTGGCTCCTTGGGCAGCAAGAAGGGTGTGAACCTTCCTGGGGCTG CTGTGGACTTGCCTGCTGTGTCGGAGAAGGACATCCAGGATCTGAAGTTTGGGGTCGAGCAGGATGTTGATATGGTGTTTGCGTCATTCATCCGCAAGGC ATCTGATGTCCATGAAGTTAGGAAGGTCCTGGGAGAGAAGGGAAAGAACATCAAGATTATCAGCAAAATCGAGAATCATGAGGGGGTTCGGAGGTTTGAT GAAATCCTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGATCTAGGCATTGAGATTCCTGCAGAGAAGGTCTTCCTTGCTCAGAAGATGATGATTG GACGGTGCAACCGAGCTGGGAAGCCTGTCATCTGTGCTACTCAGATGCTGGAGAGCATGATCAAGAAGCCCCGCCCCACTCGGGCTGAAGGCAGTGATGT GGCCAATGCAGTCCTGGATGGAGCCGACTGCATCATGCTGTCTGGAGAAACAGCCAAAGGGGACTATCCTCTGGAGGCTGTGCGCATGCAGCACCTGATA GCTCGTGAGGCTGAGGCAGCCATGTTCCACCGCAAGCTGTTTGAAGAACTTGTGCGAGCCTCAAGTCACTCCACAGACCTCATGGAAGCCATGGCCATGG GCAGCGTGGAGGCTTCTTATAAGTGTTTAGCAGCAGCTTTGATAGTTCTGACGGAGTCTGGCAGGTCTGCTCACCAGGTGGCCAGATACCGCCCACGTGC CCCCATCATTGCTGTGACCCGGAATCCCCAGACAGCTCGTCAGGCCCACCTGTACCGTGGCATCTTCCCTGTGCTGTGCAAGGACCCAGTCCAGGAGGCC TGGGCTGAGGACGTGGACCTCCGGGTGAACTTTGCCATGAATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGAT GGCGCCCTGGCTCCGGCTTCACCAACACCATGCGTGTTGTTCCTGTGCCGTGATGGACCCCAGAGCCCCTCCTCCAGCCCCTGTCCCACCCCCTTCCCCC AGCCCATCCATTAGGCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAACGCCTCACTG AAACATGGCTGTGTTTGCAGCCTGCTCTAGTGGGACAGCCCAGAGCCTGGCTGCCCATCATGTGGCCCCACCCAATCAAGGGAAGAAGGAGGAATGCTGG ACTGGAGGCCCCTGGAGCCAGATGGCAAGAGGGTGACAGCTTCCTTTCCTGTGTGTACTCTGTCCAGTTCCTTTAGAAAAAATGGATGCCCAGAGGACTC CCAACCCTGGCTTGGGGTCAAGAAACAGCCAGCAAGAGTTAGGGGCCTTAGGGCACTGGGCTGTTGTTCCATTGAAGCCGACTCTGGCCCTGGCCCTTAC TTGCTTCTCTAGCTCTCTAGGCCTCTCCAGTTTGCACCTGTCCCCACCCTCCACTCAGCTGTCCTGCAGCAAACACTCCACCCTCCACCTTCCATTTTCC CCCACTACTGCAGCACCTCCAGGCCTGTTGCTATAGAGCCTACCTGTATGTCAATAAACAACAGCTGAAGCACC

refseq_PKM2.F1 (forward primer)
refseq_PKM2.R1 (reverse primer)
Alternative donor

Frame 0

RRASS*R*LRPRGRRGRGPGRPEPGLGRGRGGG GIAAARSGITLRLRQWLLAQQLHAPWLRISSSLQ RSPSRSAPEVSGAGGYAISPHQGPVARLVRNPG APAGPGHAGRAAQDPGRLGCSGAQREENGSSRL GPALQKSPAPFLPDQDLSSHVEAP**SRDCLHSD PAAARSHG*HIPGAHVPPGH*FTTHHSPEHWHH LYHWPSFPISGDVEGDD*VWNECGSSELLSWNS *VPCGDHQECAHSHGKLCF*PHPLPARCCGSRH* RT*DPNWAHQGQRHCRGGAEEGSHSQNHAG*RL HGKV*REHPVAGLQEHLQGGGSGQQDLRG*WAY FSPGEAERCRLPGDGGGKWWLLGQQEGCEPSWGC CGLACCVGEGHPGSEVWGRAGC*YGVCVIHPQG I*CP*S*EGPGREGKEHQDYQQNRES*GGSEV* *NPGGQ*WDHGGSW*SRH*DSCREGLPCSEDDDW TVQPSWEACHLCYSDAGEHDQEAPPHSG*RQ*C GQCSPGWSRLHHAVWRNSQRGLSSGGCAHAAPD SS*G*GSHVPPQAV*RTCASLKSLHRPHGSHGHG QRGGFL*VFSSSFDSSDGVWQVCSPGGQIPPTC PHHCCDPESPDSSSGPPVPWHLPCAVQGPSPGG LG*GRGPPGELCHECWQGPRLLQEGRCGHCADRM APWLRLHQHHACCSCAVMDPRAPPPAPVPPPSP SPSIRPATLVELTLGCNVALVGWDTREEDQRLT ETWLCLQPALVGQPRAWLPIMWPHPIKGRRRNAG LEAPGARWQEGDSFLSCVYSVQFL*KKWMPRGL PTLAWGQETASKS*GP*GTGLLFH*SRLWPWPL LASLAL*ASPVCTCPHPPLSCPAANTPPSTFHFP PLLQHLQACCYRAYLYVNKQQLKH

Frame 1

AALPPEGDCARGDAGGGARVARSRDWAEGGAAE GLRRPAAG*P*G*GSGSLHSSCTRRGSGSLRLC SVARVGQRRR*AVQEATPSVPTKGQSPG*CGIPA RRPAPGTQAGRRRIQGVWDAVELRERRTAPHAW GLLFRSPQRRSFQIRTSAAMSKPHSEAGTAFIQ TQQLHAAMADTFLEHMCRLDIDSPPITARNTGII CTIGPASRSVETLKEMIKSGMNVARLNFSHGTH EYHAETIKNVRTATESFASDPILYRPVAVALDT KGPEIRTGLIKGSGTAEVELKKGATLKITLDNAY MEKCDENILWLDYKNICKVVEVGSKIYVDDGLI SLQVKQKGADFLVTEVENGGSLGSKKGVNLPGA AVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKA SDVHEVRKVLGEKGKNIKIISKIENHEGVRRFD EILEASDGIMVARGDLGIEIPAEKVFLAQKMMI GRCNRAGKPVICATQMLESMIKKPRPTRAEGSDV ANAVLDGADCIMLSGETAKGDYPLEAVRMQHLI AREAEAAMFHRKLFEELVRASSHSTDLMEAMAM GSVEASYKCLAAALIVLTESGRSAHQVARYRPRA PIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEA WAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTG WRPGSGFTNTMRVVPVP*WTPEPLLQPLSHPLPP AHPLGQQRL*NSLWAVTWHW*VGTPGKKINASL KHGCVCSLL*WDSPEPGCPSCGPTQSREEGGML DWRPLEPDGKRVTASFPVCTLSSSFRKNGCPEDS QPWLGVKKQPARVRGLRALGCCSIEADSGPGPY LLL*LSRPLQFAPVPTLHSAVLQQTLHPPPSIF PHYCSTSRPVAIEPTCMSINNS*ST

Frame 2

PRFLLKVTAPAGTQGAGPGSPGAGIGQRAGRRR DCGGPQRDNLEAEAVAPCTAAARAVAPDLFVFA A*PESVSAGGERCRRLRHQSPPRASRPASAESR RAGRPRARRQGGAGSRASGMQWSSERGERLLTPG ACSSEVPSAVPSRSGPQQPCRSPIVKPGLPSFR PSSCTQPWLTHSWSTCAAWTLIHHPSQPGTLAS SVPLAQLPDQWRR*RR*LSLE*MWLV*TSLMELM STMRRPSRMCAQPRKALLLTPSSTGPLLWL*TL KDLRSELGSSRAAALQRWS*RREPLSKSRWITP TWKSVTRTSCGWTTRTSARWWKWAARSTWMMGLF LSR*SRKVPTSW*RRWKMVAPWAARRV*TFLGL LWTCLLCRRRTSRI*SLGSSRMLIWCLRHSSAR HLMSMKLGRSWERRERTSRLSAKSRIMRGFGGLM KSWRPVMGSWWLVVI*ALRFLQRRSSLLRR**L DGATELGSLSSVLLRCWRA*SRSPAPLGLKAVM WPMQSWMEPTASCCLEKQPKGTILWRLCACST** LVRLRQPCSTASCLKNLCEPQVTPQTSWKPWPW AAWRLLISV*QQL**F*RSLAGLLTRWPDTAHV PPSLL*PGIPRQLVRPTCTVASSLCCARTQSRRP GLRTWTSG*TLP*MLARPEASSRREMWSLC*PD GALAPASPTPCVLFLCRDGPQSPSSSPCPTPFP QPIH*ASNACRTHSGL*RGTGRLGHQGRRSTPH* NMAVFAACSSGTAQSLAAHHVAPPNQGKKEECW TGGPWSQMARG*QLPFLCVLCPVPLEKMDAQRT PNPGLGSRNSQQELGALGHWAVVPLKPTLALALT CFSSSLGLSSLHLSPPSTQLSCSKHSTLHLPFS PTTAAPPGLLL*SLPVCQ*TTAEA