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Sequences

Sequence NM_194323

CCGTGAGTTCTGCCCAGGCCCTGTGAGCTCACCAGAGCCACAGACTCACAGCCCAGAGGTGGCTTCTTCCTTCAGGAACTGAAGAACCCCCATGAACACC AACATCTCCAGGTTCTGAGAACAGAACCTGGGAAATTGATGACTTCCTCATGATGACCGATACTCAGGATGGCCCTAGCGAGAGCTCCCAGATCATGAGG AAGAAGGCCTGAACGACATACAGGAGATGATCAAAACGGAGAAGTCCTACCCTGAGCGTCGCCTGCGGGGCGTCCTGGAGGAGCTGAGCTGTGGCTGCTG CCGCTTCCTCTCCCTCGCTGACAAGGACCAGGGCCACTCATCCCGCACCAGGCTTGACCGGGAGCGCCTCAAGTCCTGCATGAGGGAGCTGGAAAACATG GGGCAGCAGGCCAGGATGCTGCGGGCCCAGGTGAAGCGGCACACGGTGCGGGACAAGCTGAGGCTGTGCCAGAACTTCCTGCAGAAGCTGCGCTTCCTGG CGGACGAGCCCCAGCACAGCATTCCCGACATCTTCATCTGGATGATGAGCAACAACAAGCGTGTCGCCTATGCCCGTGTGCCCTCCAAGGACCTGCTCTT CTCCATCGTGGAGGAGGAGACTGGCAAGGACTGCGCCAAGGTCAAGACGCTCTTCCTTAAGCTGCCAGGGAAGCGGGGCTTCGGCTCGGCAGGCTGGACA GTGCAGGCCAAGGTGGAGCTGTACCTGTGGCTGGGCCTCAGCAAACAGCGCAAGGAGTTCCTGTGCGGCCTGCCCTGTGGCTTCCAGGAGGTCAAGGCAG CCCAGGGCCTGGGCCTGCATGCCTTCCCACCCGTCAGCCTGGTCTACACCAAGAAGCAGGCGTTCCAGCTCCGAGCGCACATGTACCAGGCCCGCAGCCT CTTTGCCGCCGACAGCAGCGGACTCTCAGACCCCTTTGCCCGCGTCTTCTTCATCAATCAGAGTCAGTGCACAGAGGTGCTGAATGAGACCCTGTGTCCC ACCTGGGACCAGATGCTGGTGTTCGACAACCTGGAGCTCTATGGTGAAGCTCATGAGCTGAGGGACGATCCGCCCATCATTGTCATTGAAATCTATGACC AGGATTCCATGGGCAAAGCTGACTTCATGGGCCGGACCTTCGCCAAACCCCTGGTGAAGATGGCAGACGAGGCGTACTGCCCACCCCGCTTCCCACCTCA GCTCGAGTACTACCAGATCTACCGTGGCAACGCCACAGCTGGAGACCTGCTGGCGGCCTTCGAGCTGCTGCAGATTGGACCAGCAGGGAAGGCTGACCTG CCCCCCATCAATGGCCCGGTGGACGTGGACCGAGGTCCCATCATGCCCGTGCCCATGGGCATCCGGCCCGTGCTCAGCAAGTACCGAGTGGAGGTGCTGT TCTGGGGCCTACGGGACCTAAAGCGGGTGAACCTGGCCCAGGTGGACCGGCCACGGGTGGACATCGAGTGTGCAGGGAAGGGGGTGCAGTCGTCCCTGAT CCACAATTATAAGAAGAACCCCAACTTCAACACCCTCGTCAAGTGGTTTGAAGTGGACCTCCCAGAGAACGAGCTGCTGCACCCGCCCTTGAACATCCGT GTGGTGGACTGCCGGGCCTTCGGTCGCTACACACTGGTGGGCTCCCATGCCGTCAGCTCCCTGCGACGCTTCATCTACCGGCCCCCAGACCGCTCGGCCC CCAGCTGGAACACCACGGGGGAGGTTGTGGTGACTATGGAGCCAGAGGTACCCATCAAGAAACTGGAGACCATGGTGAAGCTGGACGCGACTTCTGAAGC TGTTGTCAAGGTGGATGTGGCTGAGGAGGAGAAGGAGAAGAAGAAGAAGAAGAAGGGCACTGCGGAGGAGCCAGAGGAGGAGGAGCCAGACGAGAGCATG CTGGACTGGTGGTCCAAGTACTTTGCCTCCATTGACACCATGAAGGAGCAACTTCGACAACAAGAGCCCTCTGGAATTGACTTGGAGGAGAAGGAGGAAG TGGACAATACCGAGGGCCTGAAGGGGTCAATGAAGGGCAAGGAGAAGGCAAGGGCTGCCAAAGAGGAGAAGAAGAAGAAAACTCAGAGCTCTGGCTCTGG CCAGGGGTCCGAGGCCCCCGAGAAGAAGAAACCCAAGATTGATGAGCTTAAGGTATACCCCAAAGAGCTGGAGTCCGAGTTTGATAACTTTGAGGACTGG CTGCACACTTTCAACTTGCTTCGGGGCAAGACCGGGGATGATGAGGATGGCTCCACCGAGGAGGAGCGCATTGTGGGACGCTTCAAGGGCTCCCTCTGCG TGTACAAAGTGCCACTCCCAGAGGACGTGTCCCGGGAAGCCGGCTACGACTCCACCTACGGCATGTTCCAGGGCATCCCGAGCAATGACCCCATCAATGT GCTGGTCCGAGTCTATGTGGTCCGGGCCACGGACCTGCACCCTGCTGACATCAACGGCAAAGCTGACCCCTACATCGCCATCCGGCTAGGCAAGACTGAC ATCCGCGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTCTTTGGGAAGTCCTTTGACATCGAGGCCTCCTTCCCCATGGAATCCATGCTGACGG TGGCTGTGTATGACTGGGACCTGGTGGGCACTGATGACCTCATTGGGGAAACCAAGATCGACCTGGAGAACCGCTTCTACAGCAAGCACCGCGCCACCTG CGGCATCGCCCAGACCTACTCCACACATGGCTACAATATCTGGCGGGACCCCATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGACGGCAAAGTG GACGGCCCCCACTTTGGGCCCCCTGGGAGAGTGAAGGTGGCCAACCGCGTCTTCACTGGGCCCTCTGAGATTGAGGACGAGAACGGTCAGAGGAAGCCCA CAGACGAGCATGTGGCGCTGTTGGCCCTGAGGCACTGGGAGGACATCCCCCGCGCAGGCTGCCGCCTGGTGCCAGAGCATGTGGAGACGAGGCCGCTGCT CAACCCCGACAAGCCGGGCATCGAGCAGGGCCGCCTGGAGCTGTGGGTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACGCCTCTGGACATCTCA CCTCGGAAGCCCAAGAAGTACGAGCTGCGGGTCATCATCTGGAACACAGATGAGGTGGTCTTGGAGGACGACGACTTCTTCACAGGGGAGAAGTCCAGTG ACATCTTCGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAGCAGGACACAGACGTCCACTACCACTCCCTCACTGGCGAGGGCAACTTCAACTGGCG CTACCTGTTCCCCTTCGACTACCTGGCGGCGGAGGAGAAGATCGTCATCTCCAAGAAGGAGTCCATGTTCTCCTGGGACGAGACCGAGTACAAGATCCCC GCGCGGCTCACCCTGCAGATCTGGGATGCGGACCACTTCTCCGCTGACGACTTCCTGGGGGCCATCGAGCTGGACCTGAACCGGTTCCCGCGGGGCGCAA AGACAGCCAAGCAGTGCACCATGGAGATGGCCACCGGGGAGGTGGACGTGCCCCTCGTGTCCATCTTCAAGCAAAAGCGCGTCAAAGGCTGGTGGCCCCT CCTGGCCCGCAATGAGAACGATGAGTTTGAGCTCACGGGCAAGGTGGAGGCTGAGCTGCATTTACTGACAGCAGAGGAGGCAGAGAAGAACCCAGTGGGC CTGGCCCGCAATGAACCTGACCCCCTAGAGAAACCCAACCGGCCCGACACGGCCTTCGTCTGGTTCCTCAACCCTCTCAAGTCCATCAAGTACCTCATCT GCACCCGGTACAAGTGGCTCATCATCAAGATCGTGCTGGCGCTGTTGGGGCTGCTCATGTTGGGGCTCTTCCTCTACAGCCTCCCTGGCTACATGGTCAA AAAGCTCCTTGGGGCATGAAGGCCGCCAGCTCCCGCCAGCCGCTCCCCAGCCCTGCCGCATTTCCTTTCAGTGGCTTGGACTCTTTCCCATCTCCCCTGG GGAGCCTGAGGAGCCCAGCGTCCACTCTTCATGCCTTGGGCCGAGCCTGCCTCCTGCTTGCGGGGGCCGCCTGTCCTCACTGCCCCAGGCTGCGGCTTGC CCAGTCCCGCCCCTCTGACCCCTGCCTGTGGGCTGGGGAGCCTTGGATGGGGTGGGGACCTGGAATGGGTCTCTCTTGCCCCACCTGGCTGAGGCGCCAC CCTTCTTCAGGCCCAGGCTCCAGAGGAAGACTCCTGAAACCCTCCCCAGGTCTTCCAAGTACAGGATTGAAGCTTTAGTGAAATTAACCAAGGACCATGG GTCAGTGCCCAGGGCTTTAAAAAGAATGAACGAGCAAAAGGTATCCCCGCCGTGACCCCTGCAGATAGCACCGGTCTTTGATCCGCAGCAGGGGCCAGAC CCTGCCCACAAGTCCCAGCGCGGCTGCTTCTGCCACTGCTGGGCTCCACTTGGCTCCTCTCACTTCCCAGGGGGTCGCCTGTCCTGCCTGTGGGTTTCCA TGGCTTCCCAGAGCTCCCTCTGCCCCAGCCAGCGCCTCCAGGCCCAGCTGAGGAGCTGTGAGAAGCAGCAGAGGGGACTCCCCATCCCGGGCACACCCTG TCCTCCCACCCCTGCCCCCTTGCCCTTCCAGCCCTTTCAGCTGCAGCTGGGAGCTGGCCCGTCAAGTGCTGCCCCTGCCTGTGTCTGGGTTTCTGTTGGC TGTTTTTCTTTTCTTGAGTGGTGATTTTTCTCTAAATAAAAGAAGTCAAGCACTGA

refseq_OTOF.F1 (forward primer)
refseq_OTOF.R1 (reverse primer)
Single exon skipping

Frame 0

P*VLPRPCELTRATDSQPRGGFFLQELKNPHEH QHLQVLRTEPGKLMTSS**PILRMALARAPRS*G RRPERHTGDDQNGEVLP*ASPAGRPGGAELWLL PLPLPR*QGPGPLIPHQA*PGAPQVLHEGAGKH GAAGQDAAGPGEAAHGAGQAEAVPELPAEAALPG GRAPAQHSRHLHLDDEQQQACRLCPCALQGPAL LHRGGGDWQGLRQGQDALP*AAREAGLRLGRLD SAGQGGAVPVAGPQQTAQGVPVRPALWLPGGQGS PGPGPACLPTRQPGLHQEAGVPAPSAHVPGPQP LCRRQQRTLRPLCPRLLHQSESVHRGAE*DPVS HLGPDAGVRQPGALW*SS*AEGRSAHHCH*NL*P GFHGQS*LHGPDLRQTPGEDGRRGVLPTPLPTS ARVLPDLPWQRHSWRPAGGLRAAADWTSREG*P APHQWPGGRGPRSHHARAHGHPARAQQVPSGGAV LGPTGPKAGEPGPGGPATGGHRVCREGGAVVPD PQL*EEPQLQHPRQVV*SGPPRERAAAPALEHP CGGLPGLRSLHTGGLPCRQLPATLHLPAPRPLGP QLEHHGGGCGDYGARGTHQETGDHGEAGRDF*S CCQGGCG*GGEGEEEEEEGHCGGARGGGARREH AGLVVQVLCLH*HHEGATSTTRALWN*LGGEGGS GQYRGPEGVNEGQGEGKGCQRGEEEENSELWLW PGVRGPREEETQD**A*GIPQRAGVRV**L*GL AAHFQLASGQDRG**GWLHRGGAHCGTLQGLPLR VQSATPRGRVPGSRLRLHLRHVPGHPEQ*PHQC AGPSLCGPGHGPAPC*HQRQS*PLHRHPARQD* HPRQGELHLQAAQPCLWEVL*HRGLLPHGIHADG GCV*LGPGGH**PHWGNQDRPGEPLLQQAPRHL RHRPDLLHTWLQYLAGPHEAQPDPDPPLQRRQS GRPPLWAPWESEGGQPRLHWAL*D*GRERSEEAH RRACGAVGPEALGGHPPRRLPPGARACGDEAAA QPRQAGHRAGPPGAVGGHVPHGHASPWDASGHL TSEAQEVRAAGHHLEHR*GGLGGRRLLHRGEVQ* HLREGVAEGPAGGQAGHRRPLPLPHWRGQLQLA LPVPLRLPGGGGEDRHLQEGVHVLLGRDRVQDP RAAHPADLGCGPLLR*RLPGGHRAGPEPVPAGRK DSQAVHHGDGHRGGGRAPRVHLQAKARQRLVAP PGPQ*ER*V*AHGQGGG*AAFTDSRGGREEPSG PGPQ*T*PPRETQPARHGLRLVPQPSQVHQVPHL HPVQVAHHQDRAGAVGAAHVGALPLQPPWLHGQ KAPWGMKAASSRQPLPSPAAFPFSGLDSFPSPL GSLRSPASTLHALGRACLLLAGAACPHCPRLRLA QSRPSDPCLWAGEPWMGWGPGMGLSCPTWLRRH PSSGPGSRGRLLKPSPGLPSTGLKL**N*PRTM GQCPGL*KE*TSKRYPRRDPCR*HRSLIRSRGQT LPTSPSAAASATAGLHLAPLTSQGVACPACGFP WLPRAPSAPASASRPS*GAVRSSRGDSPSRAHP VLPPLPPCPSSPFSCSWELARQVLPLPVSGFLLA VFLFLSGDFSLNKRSQAL

Frame 1

REFCPGPVSSPEPQTHSPEVASSFRN*RTPMNT NISRF*EQNLGN**LPHDDRYSGWP*RELPDHE EEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCC RFLSLADKDQGHSSRTRLDRERLKSCMRELENM GQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFL ADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLF SIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWT VQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKA AQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSL FAADSSGLSDPFARVFFINQSQCTEVLNETLCP TWDQMLVFDNLELYGEAHELRDDPPIIVIEIYD QDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQ LEYYQIYRGNATAGDLLAAFELLQIGPAGKADL PPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVL FWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLI HNYKKNPNFNTLVKWFEVDLPENELLHPPLNIR VVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSA PSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEA VVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESM LDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEE VDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSG QGSEAPEKKKPKIDELKVYPKELESEFDNFEDW LHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLC VYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINV LVRVYVVRATDLHPADINGKADPYIAIRLGKTD IRDKENYISKQLNPVFGKSFDIEASFPMESMLT VAVYDWDLVGTDDLIGETKIDLENRFYSKHRATC GIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKV DGPHFGPPGRVKVANRVFTGPSEIEDENGQRKP TDEHVALLALRHWEDIPRAGCRLVPEHVETRPLL NPDKPGIEQGRLELWVDMFPMDMPAPGTPLDIS PRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSS DIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWR YLFPFDYLAAEEKIVISKKESMFSWDETEYKIP ARLTLQIWDADHFSADDFLGAIELDLNRFPRGA KTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPL LARNENDEFELTGKVEAELHLLTAEEAEKNPVG LARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLI CTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVK KLLGA*RPPAPASRSPALPHFLSVAWTLSHLPW GA*GAQRPLFMPWAEPASCLRGPPVLTAPGCGL PSPAPLTPACGLGSLGWGGDLEWVSLAPPG*GAT LLQAQAPEEDS*NPPQVFQVQD*SFSEINQGPW VSAQGFKKNERAKGIPAVTPADSTGL*SAAGAR PCPQVPARLLLPLLGSTWLLSLPRGSPVLPVGFH GFPELPLPQPAPPGPAEEL*EAAEGTPHPGHTL SSHPCPLALPALSAAAGSWPVKCCPCLCLGFCW LFFFS*VVIFL*IKEVKH*

Frame 2

VSSAQAL*AHQSHRLTAQRWLLPSGTEEPP*TP TSPGSENRTWEIDDFLMMTDTQDGPSESSQIMR KKA*TTYRR*SKRRSPTLSVACGASWRS*AVAA AASSPSLTRTRATHPAPGLTGSASSPA*GSWKTW GSRPGCCGPR*SGTRCGTS*GCARTSCRSCASW RTSPSTAFPTSSSG**ATTSVSPMPVCPPRTCS SPSWRRRLARTAPRSRRSSLSCQGSGASARQAGQ CRPRWSCTCGWASANSARSSCAACPVASRRSRQ PRAWACMPSHPSAWSTPRSRRSSSERTCTRPAA SLPPTAADSQTPLPASSSSIRVSAQRC*MRPCVP PGTRCWCSTTWSSMVKLMS*GTIRPSLSLKSMT RIPWAKLTSWAGPSPNPW*RWQTRRTAHPASHL SSSTTRSTVATPQLETCWRPSSCCRLDQQGRLTC PPSMARWTWTEVPSCPCPWASGPCSASTEWRCC SGAYGT*SG*TWPRWTGHGWTSSVQGRGCSRP* STIIRRTPTSTPSSSGLKWTSQRTSCCTRP*TSV WWTAGPSVATHWWAPMPSAPCDASSTGPQTARP PAGTPRGRLW*LWSQRYPSRNWRPW*SWTRLLK LLSRWMWLRRRRRRRRRRRALRRSQRRRSQTRAC WTGGPSTLPPLTP*RSNFDNKSPLELTWRRRRK WTIPRA*RGQ*RARRRQGLPKRRRRRKLRALAL ARGPRPPRRRNPRLMSLRYTPKSWSPSLITLRTG CTLSTCFGARPGMMRMAPPRRSALWDASRAPSA CTKCHSQRTCPGKPATTPPTACSRASRAMTPSM CWSESMWSGPRTCTLLTSTAKLTPTSPSG*ARLT SATRRTTSPSSSTLSLGSPLTSRPPSPWNPC*R WLCMTGTWWALMTSLGKPRSTWRTASTASTAPP AASPRPTPHMATISGGTP*SPARS*PASAKTAKW TAPTLGPLGE*RWPTASSLGPLRLRTRTVRGSP QTSMWRCWP*GTGRTSPAQAAAWCQSMWRRGRC STPTSRASSRAAWSCGWTCSPWTCQPLGRLWTSH LGSPRSTSCGSSSGTQMRWSWRTTTSSQGRSPV TSS*GGG*RASRRTSRTQTSTTTPSLARATSTG ATCSPSTTWRRRRRSSSPRRSPCSPGTRPSTRSP RGSPCRSGMRTTSPLTTSWGPSSWT*TGSRGAQ RQPSSAPWRWPPGRWTCPSCPSSSKSASKAGGP SWPAMRTMSLSSRARWRLSCIY*QQRRQRRTQWA WPAMNLTP*RNPTGPTRPSSGSSTLSSPSSTSS APGTSGSSSRSCWRCWGCSCWGSSSTASLATWS KSSLGHEGRQLPPAAPQPCRISFQWLGLFPISPG EPEEPSVHSSCLGPSLPPACGGRLSSLPQAAAC PVPPL*PLPVGWGALDGVGTWNGSLLPHLAEAP PFFRPRLQRKTPETLPRSSKYRIEALVKLTKDHG SVPRALKRMNEQKVSPP*PLQIAPVFDPQQGPD PAHKSQRGCFCHCWAPLGSSHFPGGRLSCLWVS MASQSSLCPSQRLQAQLRSCEKQQRGLPIPGTPC PPTPAPLPFQPFQLQLGAGPSSAAPACVWVSVG CFSFLEW*FFSK*KKSST

Sequence NM_194322

CCGTGAGTTCTGCCCAGGCCCTGTGAGCTCACCAGAGCCACAGACTCACAGCCCAGAGGTGGCTTCTTCCTTCAGGAACTGAAGAACCCCCATGAACACC AACATCTCCAGGTTCTGAGAACAGAACCTGGGAAATTGATGACTTCCTCATGATGACCGATACTCAGGATGGCCCTAGCGAGAGCTCCCAGATCATGAGG TCCCTCACTCCCCTGATCAACAGGGAGGAGGCATTTGGGGAGGCTGGGGAGGCGGGGCTGTGGCCCAGCATCACCCACACTCCTGATTCACAGGAAGAAG GCCTGAACGACATACAGGAGATGATCAAAACGGAGAAGTCCTACCCTGAGCGTCGCCTGCGGGGCGTCCTGGAGGAGCTGAGCTGTGGCTGCTGCCGCTT CCTCTCCCTCGCTGACAAGGACCAGGGCCACTCATCCCGCACCAGGCTTGACCGGGAGCGCCTCAAGTCCTGCATGAGGGAGCTGGAAAACATGGGGCAG CAGGCCAGGATGCTGCGGGCCCAGGTGAAGCGGCACACGGTGCGGGACAAGCTGAGGCTGTGCCAGAACTTCCTGCAGAAGCTGCGCTTCCTGGCGGACG AGCCCCAGCACAGCATTCCCGACATCTTCATCTGGATGATGAGCAACAACAAGCGTGTCGCCTATGCCCGTGTGCCCTCCAAGGACCTGCTCTTCTCCAT CGTGGAGGAGGAGACTGGCAAGGACTGCGCCAAGGTCAAGACGCTCTTCCTTAAGCTGCCAGGGAAGCGGGGCTTCGGCTCGGCAGGCTGGACAGTGCAG GCCAAGGTGGAGCTGTACCTGTGGCTGGGCCTCAGCAAACAGCGCAAGGAGTTCCTGTGCGGCCTGCCCTGTGGCTTCCAGGAGGTCAAGGCAGCCCAGG GCCTGGGCCTGCATGCCTTCCCACCCGTCAGCCTGGTCTACACCAAGAAGCAGGCGTTCCAGCTCCGAGCGCACATGTACCAGGCCCGCAGCCTCTTTGC CGCCGACAGCAGCGGACTCTCAGACCCCTTTGCCCGCGTCTTCTTCATCAATCAGAGTCAGTGCACAGAGGTGCTGAATGAGACCCTGTGTCCCACCTGG GACCAGATGCTGGTGTTCGACAACCTGGAGCTCTATGGTGAAGCTCATGAGCTGAGGGACGATCCGCCCATCATTGTCATTGAAATCTATGACCAGGATT CCATGGGCAAAGCTGACTTCATGGGCCGGACCTTCGCCAAACCCCTGGTGAAGATGGCAGACGAGGCGTACTGCCCACCCCGCTTCCCACCTCAGCTCGA GTACTACCAGATCTACCGTGGCAACGCCACAGCTGGAGACCTGCTGGCGGCCTTCGAGCTGCTGCAGATTGGACCAGCAGGGAAGGCTGACCTGCCCCCC ATCAATGGCCCGGTGGACGTGGACCGAGGTCCCATCATGCCCGTGCCCATGGGCATCCGGCCCGTGCTCAGCAAGTACCGAGTGGAGGTGCTGTTCTGGG GCCTACGGGACCTAAAGCGGGTGAACCTGGCCCAGGTGGACCGGCCACGGGTGGACATCGAGTGTGCAGGGAAGGGGGTGCAGTCGTCCCTGATCCACAA TTATAAGAAGAACCCCAACTTCAACACCCTCGTCAAGTGGTTTGAAGTGGACCTCCCAGAGAACGAGCTGCTGCACCCGCCCTTGAACATCCGTGTGGTG GACTGCCGGGCCTTCGGTCGCTACACACTGGTGGGCTCCCATGCCGTCAGCTCCCTGCGACGCTTCATCTACCGGCCCCCAGACCGCTCGGCCCCCAGCT GGAACACCACGGTCAGGCTTCTCCGGCGCTGCCGTGTGCTGTGCAATGGGGGCTCCTCCTCTCACTCCACAGGGGAGGTTGTGGTGACTATGGAGCCAGA GGTACCCATCAAGAAACTGGAGACCATGGTGAAGCTGGACGCGACTTCTGAAGCTGTTGTCAAGGTGGATGTGGCTGAGGAGGAGAAGGAGAAGAAGAAG AAGAAGAAGGGCACTGCGGAGGAGCCAGAGGAGGAGGAGCCAGACGAGAGCATGCTGGACTGGTGGTCCAAGTACTTTGCCTCCATTGACACCATGAAGG AGCAACTTCGACAACAAGAGCCCTCTGGAATTGACTTGGAGGAGAAGGAGGAAGTGGACAATACCGAGGGCCTGAAGGGGTCAATGAAGGGCAAGGAGAA GGCAAGGGCTGCCAAAGAGGAGAAGAAGAAGAAAACTCAGAGCTCTGGCTCTGGCCAGGGGTCCGAGGCCCCCGAGAAGAAGAAACCCAAGATTGATGAG CTTAAGGTATACCCCAAAGAGCTGGAGTCCGAGTTTGATAACTTTGAGGACTGGCTGCACACTTTCAACTTGCTTCGGGGCAAGACCGGGGATGATGAGG ATGGCTCCACCGAGGAGGAGCGCATTGTGGGACGCTTCAAGGGCTCCCTCTGCGTGTACAAAGTGCCACTCCCAGAGGACGTGTCCCGGGAAGCCGGCTA CGACTCCACCTACGGCATGTTCCAGGGCATCCCGAGCAATGACCCCATCAATGTGCTGGTCCGAGTCTATGTGGTCCGGGCCACGGACCTGCACCCTGCT GACATCAACGGCAAAGCTGACCCCTACATCGCCATCCGGCTAGGCAAGACTGACATCCGCGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTCT TTGGGAAGTCCTTTGACATCGAGGCCTCCTTCCCCATGGAATCCATGCTGACGGTGGCTGTGTATGACTGGGACCTGGTGGGCACTGATGACCTCATTGG GGAAACCAAGATCGACCTGGAGAACCGCTTCTACAGCAAGCACCGCGCCACCTGCGGCATCGCCCAGACCTACTCCACACATGGCTACAATATCTGGCGG GACCCCATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGACGGCAAAGTGGACGGCCCCCACTTTGGGCCCCCTGGGAGAGTGAAGGTGGCCAACC GCGTCTTCACTGGGCCCTCTGAGATTGAGGACGAGAACGGTCAGAGGAAGCCCACAGACGAGCATGTGGCGCTGTTGGCCCTGAGGCACTGGGAGGACAT CCCCCGCGCAGGCTGCCGCCTGGTGCCAGAGCATGTGGAGACGAGGCCGCTGCTCAACCCCGACAAGCCGGGCATCGAGCAGGGCCGCCTGGAGCTGTGG GTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACGCCTCTGGACATCTCACCTCGGAAGCCCAAGAAGTACGAGCTGCGGGTCATCATCTGGAACA CAGATGAGGTGGTCTTGGAGGACGACGACTTCTTCACAGGGGAGAAGTCCAGTGACATCTTCGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAGCA GGACACAGACGTCCACTACCACTCCCTCACTGGCGAGGGCAACTTCAACTGGCGCTACCTGTTCCCCTTCGACTACCTGGCGGCGGAGGAGAAGATCGTC ATCTCCAAGAAGGAGTCCATGTTCTCCTGGGACGAGACCGAGTACAAGATCCCCGCGCGGCTCACCCTGCAGATCTGGGATGCGGACCACTTCTCCGCTG ACGACTTCCTGGGGGCCATCGAGCTGGACCTGAACCGGTTCCCGCGGGGCGCAAAGACAGCCAAGCAGTGCACCATGGAGATGGCCACCGGGGAGGTGGA CGTGCCCCTCGTGTCCATCTTCAAGCAAAAGCGCGTCAAAGGCTGGTGGCCCCTCCTGGCCCGCAATGAGAACGATGAGTTTGAGCTCACGGGCAAGGTG GAGGCTGAGCTGCATTTACTGACAGCAGAGGAGGCAGAGAAGAACCCAGTGGGCCTGGCCCGCAATGAACCTGACCCCCTAGAGAAACCCAACCGGCCCG ACACGAGCTTCATCTGGTTCCTGAACCCTCTCAAGTCGGCTCGCTACTTCTTGTGGCACACGTATCGCTGGCTGCTCCTCAAACTGTTGCTGCTCCTGCT GCTGCTCCTCCTCCTCGCCCTGTTCCTCTACTCTGTGCCTGGCTACCTGGTCAAGAAAATCCTCGGGGCCTGAGCCCAGTGGCCTCCTGGCCGGCCCGAC ACGGCCTTCGTCTGGTTCCTCAACCCTCTCAAGTCCATCAAGTACCTCATCTGCACCCGGTACAAGTGGCTCATCATCAAGATCGTGCTGGCGCTGTTGG GGCTGCTCATGTTGGGGCTCTTCCTCTACAGCCTCCCTGGCTACATGGTCAAAAAGCTCCTTGGGGCATGAAGGCCGCCAGCTCCCGCCAGCCGCTCCCC AGCCCTGCCGCATTTCCTTTCAGTGGCTTGGACTCTTTCCCATCTCCCCTGGGGAGCCTGAGGAGCCCAGCGTCCACTCTTCATGCCTTGGGCCGAGCCT GCCTCCTGCTTGCGGGGGCCGCCTGTCCTCACTGCCCCAGGCTGCGGCTTGCCCAGTCCCGCCCCTCTGACCCCTGCCTGTGGGCTGGGGAGCCTTGGAT GGGGTGGGGACCTGGAATGGGTCTCTCTTGCCCCACCTGGCTGAGGCGCCACCCTTCTTCAGGCCCAGGCTCCAGAGGAAGACTCCTGAAACCCTCCCCA GGTCTTCCAAGTACAGGATTGAAGCTTTAGTGAAATTAACCAAGGACCATGGGTCAGTGCCCAGGGCTTTAAAAAGAATGAACGAGCAAAAGGTATCCCC GCCGTGACCCCTGCAGATAGCACCGGTCTTTGATCCGCAGCAGGGGCCAGACCCTGCCCACAAGTCCCAGCGCGGCTGCTTCTGCCACTGCTGGGCTCCA CTTGGCTCCTCTCACTTCCCAGGGGGTCGCCTGTCCTGCCTGTGGGTTTCCATGGCTTCCCAGAGCTCCCTCTGCCCCAGCCAGCGCCTCCAGGCCCAGC TGAGGAGCTGTGAGAAGCAGCAGAGGGGACTCCCCATCCCGGGCACACCCTGTCCTCCCACCCCTGCCCCCTTGCCCTTCCAGCCCTTTCAGCTGCAGCT GGGAGCTGGCCCGTCAAGTGCTGCCCCTGCCTGTGTCTGGGTTTCTGTTGGCTGTTTTTCTTTTCTTGAGTGGTGATTTTTCTCTAAATAAAAGAAGTCA AGCACTGA

refseq_OTOF.F1 (forward primer)
refseq_OTOF.R1 (reverse primer)
Single exon skipping

Frame 0

P*VLPRPCELTRATDSQPRGGFFLQELKNPHEH QHLQVLRTEPGKLMTSS**PILRMALARAPRS*G PSLP*STGRRHLGRLGRRGCGPASPTLLIHRKK A*TTYRR*SKRRSPTLSVACGASWRS*AVAAAA SSPSLTRTRATHPAPGLTGSASSPA*GSWKTWGS RPGCCGPR*SGTRCGTS*GCARTSCRSCASWRT SPSTAFPTSSSG**ATTSVSPMPVCPPRTCSSP SWRRRLARTAPRSRRSSLSCQGSGASARQAGQCR PRWSCTCGWASANSARSSCAACPVASRRSRQPR AWACMPSHPSAWSTPRSRRSSSERTCTRPAASL PPTAADSQTPLPASSSSIRVSAQRC*MRPCVPPG TRCWCSTTWSSMVKLMS*GTIRPSLSLKSMTRI PWAKLTSWAGPSPNPW*RWQTRRTAHPASHLSS STTRSTVATPQLETCWRPSSCCRLDQQGRLTCPP SMARWTWTEVPSCPCPWASGPCSASTEWRCCSG AYGT*SG*TWPRWTGHGWTSSVQGRGCSRP*ST IIRRTPTSTPSSSGLKWTSQRTSCCTRP*TSVWW TAGPSVATHWWAPMPSAPCDASSTGPQTARPPA GTPRSGFSGAAVCCAMGAPPLTPQGRLW*LWSQ RYPSRNWRPW*SWTRLLKLLSRWMWLRRRRRRRR RRRALRRSQRRRSQTRACWTGGPSTLPPLTP*R SNFDNKSPLELTWRRRRKWTIPRA*RGQ*RARR RQGLPKRRRRRKLRALALARGPRPPRRRNPRLMS LRYTPKSWSPSLITLRTGCTLSTCFGARPGMMR MAPPRRSALWDASRAPSACTKCHSQRTCPGKPA TTPPTACSRASRAMTPSMCWSESMWSGPRTCTLL TSTAKLTPTSPSG*ARLTSATRRTTSPSSSTLS LGSPLTSRPPSPWNPC*RWLCMTGTWWALMTSL GKPRSTWRTASTASTAPPAASPRPTPHMATISGG TP*SPARS*PASAKTAKWTAPTLGPLGE*RWPT ASSLGPLRLRTRTVRGSPQTSMWRCWP*GTGRT SPAQAAAWCQSMWRRGRCSTPTSRASSRAAWSCG WTCSPWTCQPLGRLWTSHLGSPRSTSCGSSSGT QMRWSWRTTTSSQGRSPVTSS*GGG*RASRRTS RTQTSTTTPSLARATSTGATCSPSTTWRRRRRSS SPRRSPCSPGTRPSTRSPRGSPCRSGMRTTSPL TTSWGPSSWT*TGSRGAQRQPSSAPWRWPPGRW TCPSCPSSSKSASKAGGPSWPAMRTMSLSSRARW RLSCIY*QQRRQRRTQWAWPAMNLTP*RNPTGP TRASSGS*TLSSRLATSCGTRIAGCSSNCCCSC CCSSSSPCSSTLCLATWSRKSSGPEPSGLLAGPT RPSSGSSTLSSPSSTSSAPGTSGSSSRSCWRCW GCSCWGSSSTASLATWSKSSLGHEGRQLPPAAP QPCRISFQWLGLFPISPGEPEEPSVHSSCLGPSL PPACGGRLSSLPQAAACPVPPL*PLPVGWGALD GVGTWNGSLLPHLAEAPPFFRPRLQRKTPETLP RSSKYRIEALVKLTKDHGSVPRALKRMNEQKVSP P*PLQIAPVFDPQQGPDPAHKSQRGCFCHCWAP LGSSHFPGGRLSCLWVSMASQSSLCPSQRLQAQ LRSCEKQQRGLPIPGTPCPPTPAPLPFQPFQLQL GAGPSSAAPACVWVSVGCFSFLEW*FFSK*KKS ST

Frame 1

REFCPGPVSSPEPQTHSPEVASSFRN*RTPMNT NISRF*EQNLGN**LPHDDRYSGWP*RELPDHE VPHSPDQQGGGIWGGWGGGAVAQHHPHS*FTGRR PERHTGDDQNGEVLP*ASPAGRPGGAELWLLPL PLPR*QGPGPLIPHQA*PGAPQVLHEGAGKHGA AGQDAAGPGEAAHGAGQAEAVPELPAEAALPGGR APAQHSRHLHLDDEQQQACRLCPCALQGPALLH RGGGDWQGLRQGQDALP*AAREAGLRLGRLDSA GQGGAVPVAGPQQTAQGVPVRPALWLPGGQGSPG PGPACLPTRQPGLHQEAGVPAPSAHVPGPQPLC RRQQRTLRPLCPRLLHQSESVHRGAE*DPVSHL GPDAGVRQPGALW*SS*AEGRSAHHCH*NL*PGF HGQS*LHGPDLRQTPGEDGRRGVLPTPLPTSAR VLPDLPWQRHSWRPAGGLRAAADWTSREG*PAP HQWPGGRGPRSHHARAHGHPARAQQVPSGGAVLG PTGPKAGEPGPGGPATGGHRVCREGGAVVPDPQ L*EEPQLQHPRQVV*SGPPRERAAAPALEHPCG GLPGLRSLHTGGLPCRQLPATLHLPAPRPLGPQL EHHGQASPALPCAVQWGLLLSLHRGGCGDYGAR GTHQETGDHGEAGRDF*SCCQGGCG*GGEGEEE EEEGHCGGARGGGARREHAGLVVQVLCLH*HHEG ATSTTRALWN*LGGEGGSGQYRGPEGVNEGQGE GKGCQRGEEEENSELWLWPGVRGPREEETQD** A*GIPQRAGVRV**L*GLAAHFQLASGQDRG**G WLHRGGAHCGTLQGLPLRVQSATPRGRVPGSRL RLHLRHVPGHPEQ*PHQCAGPSLCGPGHGPAPC *HQRQS*PLHRHPARQD*HPRQGELHLQAAQPCL WEVL*HRGLLPHGIHADGGCV*LGPGGH**PHW GNQDRPGEPLLQQAPRHLRHRPDLLHTWLQYLA GPHEAQPDPDPPLQRRQSGRPPLWAPWESEGGQP RLHWAL*D*GRERSEEAHRRACGAVGPEALGGH PPRRLPPGARACGDEAAAQPRQAGHRAGPPGAV GGHVPHGHASPWDASGHLTSEAQEVRAAGHHLEH R*GGLGGRRLLHRGEVQ*HLREGVAEGPAGGQA GHRRPLPLPHWRGQLQLALPVPLRLPGGGGEDR HLQEGVHVLLGRDRVQDPRAAHPADLGCGPLLR* RLPGGHRAGPEPVPAGRKDSQAVHHGDGHRGGG RAPRVHLQAKARQRLVAPPGPQ*ER*V*AHGQG GG*AAFTDSRGGREEPSGPGPQ*T*PPRETQPAR HELHLVPEPSQVGSLLLVAHVSLAAPQTVAAPA AAPPPRPVPLLCAWLPGQENPRGLSPVASWPAR HGLRLVPQPSQVHQVPHLHPVQVAHHQDRAGAVG AAHVGALPLQPPWLHGQKAPWGMKAASSRQPLP SPAAFPFSGLDSFPSPLGSLRSPASTLHALGRA CLLLAGAACPHCPRLRLAQSRPSDPCLWAGEPWM GWGPGMGLSCPTWLRRHPSSGPGSRGRLLKPSP GLPSTGLKL**N*PRTMGQCPGL*KE*TSKRYP RRDPCR*HRSLIRSRGQTLPTSPSAAASATAGLH LAPLTSQGVACPACGFPWLPRAPSAPASASRPS *GAVRSSRGDSPSRAHPVLPPLPPCPSSPFSCS WELARQVLPLPVSGFLLAVFLFLSGDFSLNKRSQ AL

Frame 2

VSSAQAL*AHQSHRLTAQRWLLPSGTEEPP*TP TSPGSENRTWEIDDFLMMTDTQDGPSESSQIMR SLTPLINREEAFGEAGEAGLWPSITHTPDSQEE GLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRF LSLADKDQGHSSRTRLDRERLKSCMRELENMGQ QARMLRAQVKRHTVRDKLRLCQNFLQKLRFLAD EPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSI VEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQ AKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQ GLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFA ADSSGLSDPFARVFFINQSQCTEVLNETLCPTW DQMLVFDNLELYGEAHELRDDPPIIVIEIYDQD SMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLE YYQIYRGNATAGDLLAAFELLQIGPAGKADLPP INGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFW GLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHN YKKNPNFNTLVKWFEVDLPENELLHPPLNIRVV DCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPS WNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPE VPIKKLETMVKLDATSEAVVKVDVAEEEKEKKK KKKGTAEEPEEEEPDESMLDWWSKYFASIDTMK EQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEK ARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDE LKVYPKELESEFDNFEDWLHTFNLLRGKTGDDE DGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGY DSTYGMFQGIPSNDPINVLVRVYVVRATDLHPA DINGKADPYIAIRLGKTDIRDKENYISKQLNPV FGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIG ETKIDLENRFYSKHRATCGIAQTYSTHGYNIWR DPMKPSQILTRLCKDGKVDGPHFGPPGRVKVAN RVFTGPSEIEDENGQRKPTDEHVALLALRHWEDI PRAGCRLVPEHVETRPLLNPDKPGIEQGRLELW VDMFPMDMPAPGTPLDISPRKPKKYELRVIIWN TDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQ DTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIV ISKKESMFSWDETEYKIPARLTLQIWDADHFSA DDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVD VPLVSIFKQKRVKGWWPLLARNENDEFELTGKV EAELHLLTAEEAEKNPVGLARNEPDPLEKPNRP DTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLL LLLLLALFLYSVPGYLVKKILGA*AQWPPGRPD TAFVWFLNPLKSIKYLICTRYKWLIIKIVLALL GLLMLGLFLYSLPGYMVKKLLGA*RPPAPASRSP ALPHFLSVAWTLSHLPWGA*GAQRPLFMPWAEP ASCLRGPPVLTAPGCGLPSPAPLTPACGLGSLG WGGDLEWVSLAPPG*GATLLQAQAPEEDS*NPPQ VFQVQD*SFSEINQGPWVSAQGFKKNERAKGIP AVTPADSTGL*SAAGARPCPQVPARLLLPLLGS TWLLSLPRGSPVLPVGFHGFPELPLPQPAPPGPA EEL*EAAEGTPHPGHTLSSHPCPLALPALSAAA GSWPVKCCPCLCLGFCWLFFFS*VVIFL*IKEV KH*

Sequence NM_194248

ATCGGAGGGGGGTCGGGAGGAGGAGGAGGAGGCAGCGGCAGAGAAGAGAGAGGCGTGTGAGCCGTGCTCCACCGGCTAGCTCCTTCCCGCTGCTCCTGCC TGGCAGTGCCAGGCAGCCCACACCAGCATGGCCTTGCTCATCCACCTCAAGACAGTCTCGGAGCTGCGGGGCAGGGGCGACCGGATCGCCAAAGTGACTT TCCGAGGGCAATCCTTCTACTCTCGGGTCCTGGAGAACTGTGAGGATGTGGCTGACTTTGATGAGACATTTCGGTGGCCGGTGGCCAGCAGCATCGACAG AAATGAGATGCTGGAGATTCAGGTTTTCAACTACAGCAAAGTCTTCAGCAACAAGCTCATCGGGACCTTCCGCATGGTGCTGCAGAAGGTGGTAGAGGAG AGCCATGTGGAGGTGACTGACACGCTGATTGATGACAACAATGCTATCATCAAGACCAGCCTGTGCGTGGAGGTCCGGTATCAGGCCACTGACGGCACAG TGGGCTCCTGGGACGATGGGGACTTCCTGGGAGATGAGTCTCTTCAAGAGGAAGAGAAGGACAGCCAAGAGACGGATGGACTGCTCCCAGGCTCCCGGCC CAGCTCCCGGCCCCCAGGAGAGAAGAGCTTCCGGAGAGCCGGGAGGAGCGTGTTCTCCGCCATGAAGCTCGGCAAAAACCGGTCTCACAAGGAGGAGCCC CAAAGACCAGATGAACCGGCGGTGCTGGAGATGGAAGACCTTGACCATCTGGCCATTCGGCTAGGAGATGGACTGGATCCCGACTCGGTGTCTCTAGCCT CAGTCACAGCTCTCACCACTAATGTCTCCAACAAGCGATCTAAGCCAGACATTAAGATGGAGCCAAGTGCTGGGCGGCCCATGGATTACCAGGTCAGCAT CACGGTGATCGAGGCCCGGCAGCTGGTGGGCTTGAACATGGACCCTGTGGTGTGCGTGGAGGTGGGTGACGACAAGAAGTACACATCCATGAAGGAGTCC ACTAACTGCCCCTATTACAACGAGTACTTCGTCTTCGACTTCCATGTCTCTCCGGATGTCATGTTTGACAAGATCATCAAGATTTCGGTGATTCACTCCA AGAACCTGCTGCGCAGTGGCACCCTGGTGGGCTCCTTCAAAATGGACGTGGGAACCGTGTACTCGCAGCCAGAGCACCAGTTCCATCACAAGTGGGCCAT CCTGTCTGACCCCGATGACATCTCCTCGGGGCTGAAGGGCTACGTGAAGTGTGACGTTGCCGTGGTGGGCAAAGGGGACAACATCAAGACGCCCCACAAG GCCAATGAGACCGACGAAGATGACATTGAGGGGAACTTGCTGCTCCCCGAGGGGGTGCCCCCCGAACGCCAGTGGGCCCGGTTCTATGTGAAAATTTACC GAGCAGAGGGGCTGCCCCGTATGAACACAAGCCTCATGGCCAATGTAAAGAAGGCTTTCATCGGTGAAAACAAGGACCTCGTGGACCCCTACGTGCAAGT CTTCTTTGCTGGCCAGAAGGGCAAGACTTCAGTGCAGAAGAGCAGCTATGAGCCCCTGTGGAATGAGCAGGTCGTCTTTACAGACCTCTTCCCCCCACTC TGCAAACGCATGAAGGTGCAGATCCGAGACTCGGACAAGGTCAACGACGTGGCCATCGGCACCCACTTCATTGACCTGCGCAAGATTTCTAATGACGGAG ACAAAGGCTTCCTGCCCACACTGGGCCCAGCCTGGGTGAACATGTACGGCTCCACACGTAACTACACGCTGCTGGATGAGCATCAGGACCTGAACGAGGG CCTGGGGGAGGGTGTGTCCTTCCGGGCCCGGCTCCTGCTGGGCCTGGCTGTGGAGATCGTAGACACCTCCAACCCTGAGCTCACCAGCTCCACAGAGGTG CAGGTGGAGCAGGCCACGCCCATCTCGGAGAGCTGTGCAGGTAAAATGGAAGAATTCTTTCTCTTTGGAGCCTTCCTGGAGGCCTCAATGATCGACCGGA GAAACGGAGACAAGCCCATCACCTTTGAGGTCACCATAGGCAACTATGGGAACGAAGTTGATGGCCTGTCCCGGCCCCAGCGGCCTCGGCCCCGGAAGGA GCCGGGGGATGAGGAAGAAGTAGACCTGATTCAGAACGCAAGTGATGACGAGGCCGGTGATGCCGGGGACCTGGCCTCAGTCTCCTCCACTCCACCAATG CGGCCCCAGGTCACCGACAGGAACTACTTCCATCTGCCCTACCTGGAGCGAAAGCCCTGCATCTACATCAAGAGCTGGTGGCCGGACCAGCGCCGCCGCC TCTACAATGCCAACATCATGGACCACATTGCCGACAAGCTGGAAGAAGGCCTGAACGACATACAGGAGATGATCAAAACGGAGAAGTCCTACCCTGAGCG TCGCCTGCGGGGCGTCCTGGAGGAGCTGAGCTGTGGCTGCTGCCGCTTCCTCTCCCTCGCTGACAAGGACCAGGGCCACTCATCCCGCACCAGGCTTGAC CGGGAGCGCCTCAAGTCCTGCATGAGGGAGCTGGAAAACATGGGGCAGCAGGCCAGGATGCTGCGGGCCCAGGTGAAGCGGCACACGGTGCGGGACAAGC TGAGGCTGTGCCAGAACTTCCTGCAGAAGCTGCGCTTCCTGGCGGACGAGCCCCAGCACAGCATTCCCGACATCTTCATCTGGATGATGAGCAACAACAA GCGTGTCGCCTATGCCCGTGTGCCCTCCAAGGACCTGCTCTTCTCCATCGTGGAGGAGGAGACTGGCAAGGACTGCGCCAAGGTCAAGACGCTCTTCCTT AAGCTGCCAGGGAAGCGGGGCTTCGGCTCGGCAGGCTGGACAGTGCAGGCCAAGGTGGAGCTGTACCTGTGGCTGGGCCTCAGCAAACAGCGCAAGGAGT TCCTGTGCGGCCTGCCCTGTGGCTTCCAGGAGGTCAAGGCAGCCCAGGGCCTGGGCCTGCATGCCTTCCCACCCGTCAGCCTGGTCTACACCAAGAAGCA GGCGTTCCAGCTCCGAGCGCACATGTACCAGGCCCGCAGCCTCTTTGCCGCCGACAGCAGCGGACTCTCAGACCCCTTTGCCCGCGTCTTCTTCATCAAT CAGAGTCAGTGCACAGAGGTGCTGAATGAGACCCTGTGTCCCACCTGGGACCAGATGCTGGTGTTCGACAACCTGGAGCTCTATGGTGAAGCTCATGAGC TGAGGGACGATCCGCCCATCATTGTCATTGAAATCTATGACCAGGATTCCATGGGCAAAGCTGACTTCATGGGCCGGACCTTCGCCAAACCCCTGGTGAA GATGGCAGACGAGGCGTACTGCCCACCCCGCTTCCCACCTCAGCTCGAGTACTACCAGATCTACCGTGGCAACGCCACAGCTGGAGACCTGCTGGCGGCC TTCGAGCTGCTGCAGATTGGACCAGCAGGGAAGGCTGACCTGCCCCCCATCAATGGCCCGGTGGACGTGGACCGAGGTCCCATCATGCCCGTGCCCATGG GCATCCGGCCCGTGCTCAGCAAGTACCGAGTGGAGGTGCTGTTCTGGGGCCTACGGGACCTAAAGCGGGTGAACCTGGCCCAGGTGGACCGGCCACGGGT GGACATCGAGTGTGCAGGGAAGGGGGTGCAGTCGTCCCTGATCCACAATTATAAGAAGAACCCCAACTTCAACACCCTCGTCAAGTGGTTTGAAGTGGAC CTCCCAGAGAACGAGCTGCTGCACCCGCCCTTGAACATCCGTGTGGTGGACTGCCGGGCCTTCGGTCGCTACACACTGGTGGGCTCCCATGCCGTCAGCT CCCTGCGACGCTTCATCTACCGGCCCCCAGACCGCTCGGCCCCCAGCTGGAACACCACGGTCAGGCTTCTCCGGCGCTGCCGTGTGCTGTGCAATGGGGG CTCCTCCTCTCACTCCACAGGGGAGGTTGTGGTGACTATGGAGCCAGAGGTACCCATCAAGAAACTGGAGACCATGGTGAAGCTGGACGCGACTTCTGAA GCTGTTGTCAAGGTGGATGTGGCTGAGGAGGAGAAGGAGAAGAAGAAGAAGAAGAAGGGCACTGCGGAGGAGCCAGAGGAGGAGGAGCCAGACGAGAGCA TGCTGGACTGGTGGTCCAAGTACTTTGCCTCCATTGACACCATGAAGGAGCAACTTCGACAACAAGAGCCCTCTGGAATTGACTTGGAGGAGAAGGAGGA AGTGGACAATACCGAGGGCCTGAAGGGGTCAATGAAGGGCAAGGAGAAGGCAAGGGCTGCCAAAGAGGAGAAGAAGAAGAAAACTCAGAGCTCTGGCTCT GGCCAGGGGTCCGAGGCCCCCGAGAAGAAGAAACCCAAGATTGATGAGCTTAAGGTATACCCCAAAGAGCTGGAGTCCGAGTTTGATAACTTTGAGGACT GGCTGCACACTTTCAACTTGCTTCGGGGCAAGACCGGGGATGATGAGGATGGCTCCACCGAGGAGGAGCGCATTGTGGGACGCTTCAAGGGCTCCCTCTG CGTGTACAAAGTGCCACTCCCAGAGGACGTGTCCCGGGAAGCCGGCTACGACTCCACCTACGGCATGTTCCAGGGCATCCCGAGCAATGACCCCATCAAT GTGCTGGTCCGAGTCTATGTGGTCCGGGCCACGGACCTGCACCCTGCTGACATCAACGGCAAAGCTGACCCCTACATCGCCATCCGGCTAGGCAAGACTG ACATCCGCGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTCTTTGGGAAGTCCTTTGACATCGAGGCCTCCTTCCCCATGGAATCCATGCTGAC GGTGGCTGTGTATGACTGGGACCTGGTGGGCACTGATGACCTCATTGGGGAAACCAAGATCGACCTGGAGAACCGCTTCTACAGCAAGCACCGCGCCACC TGCGGCATCGCCCAGACCTACTCCACACATGGCTACAATATCTGGCGGGACCCCATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGACGGCAAAG TGGACGGCCCCCACTTTGGGCCCCCTGGGAGAGTGAAGGTGGCCAACCGCGTCTTCACTGGGCCCTCTGAGATTGAGGACGAGAACGGTCAGAGGAAGCC CACAGACGAGCATGTGGCGCTGTTGGCCCTGAGGCACTGGGAGGACATCCCCCGCGCAGGCTGCCGCCTGGTGCCAGAGCATGTGGAGACGAGGCCGCTG CTCAACCCCGACAAGCCGGGCATCGAGCAGGGCCGCCTGGAGCTGTGGGTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACGCCTCTGGACATCT CACCTCGGAAGCCCAAGAAGTACGAGCTGCGGGTCATCATCTGGAACACAGATGAGGTGGTCTTGGAGGACGACGACTTCTTCACAGGGGAGAAGTCCAG TGACATCTTCGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAGCAGGACACAGACGTCCACTACCACTCCCTCACTGGCGAGGGCAACTTCAACTGG CGCTACCTGTTCCCCTTCGACTACCTGGCGGCGGAGGAGAAGATCGTCATCTCCAAGAAGGAGTCCATGTTCTCCTGGGACGAGACCGAGTACAAGATCC CCGCGCGGCTCACCCTGCAGATCTGGGATGCGGACCACTTCTCCGCTGACGACTTCCTGGGGGCCATCGAGCTGGACCTGAACCGGTTCCCGCGGGGCGC AAAGACAGCCAAGCAGTGCACCATGGAGATGGCCACCGGGGAGGTGGACGTGCCCCTCGTGTCCATCTTCAAGCAAAAGCGCGTCAAAGGCTGGTGGCCC CTCCTGGCCCGCAATGAGAACGATGAGTTTGAGCTCACGGGCAAGGTGGAGGCTGAGCTGCATTTACTGACAGCAGAGGAGGCAGAGAAGAACCCAGTGG GCCTGGCCCGCAATGAACCTGACCCCCTAGAGAAACCCAACCGGCCCGACACGAGCTTCATCTGGTTCCTGAACCCTCTCAAGTCGGCTCGCTACTTCTT GTGGCACACGTATCGCTGGCTGCTCCTCAAACTGTTGCTGCTCCTGCTGCTGCTCCTCCTCCTCGCCCTGTTCCTCTACTCTGTGCCTGGCTACCTGGTC AAGAAAATCCTCGGGGCCTGAGCCCAGTGGCCTCCTGGCCGGCCCGACACGGCCTTCGTCTGGTTCCTCAACCCTCTCAAGTCCATCAAGTACCTCATCT GCACCCGGTACAAGTGGCTCATCATCAAGATCGTGCTGGCGCTGTTGGGGCTGCTCATGTTGGGGCTCTTCCTCTACAGCCTCCCTGGCTACATGGTCAA AAAGCTCCTTGGGGCATGAAGGCCGCCAGCTCCCGCCAGCCGCTCCCCAGCCCTGCCGCATTTCCTTTCAGTGGCTTGGACTCTTTCCCATCTCCCCTGG GGAGCCTGAGGAGCCCAGCGTCCACTCTTCATGCCTTGGGCCGAGCCTGCCTCCTGCTTGCGGGGGCCGCCTGTCCTCACTGCCCCAGGCTGCGGCTTGC CCAGTCCCGCCCCTCTGACCCCTGCCTGTGGGCTGGGGAGCCTTGGATGGGGTGGGGACCTGGAATGGGTCTCTCTTGCCCCACCTGGCTGAGGCGCCAC CCTTCTTCAGGCCCAGGCTCCAGAGGAAGACTCCTGAAACCCTCCCCAGGTCTTCCAAGTACAGGATTGAAGCTTTAGTGAAATTAACCAAGGACCATGG GTCAGTGCCCAGGGCTTTAAAAAGAATGAACGAGCAAAAGGTATCCCCGCCGTGACCCCTGCAGATAGCACCGGTCTTTGATCCGCAGCAGGGGCCAGAC CCTGCCCACAAGTCCCAGCGCGGCTGCTTCTGCCACTGCTGGGCTCCACTTGGCTCCTCTCACTTCCCAGGGGGTCGCCTGTCCTGCCTGTGGGTTTCCA TGGCTTCCCAGAGCTCCCTCTGCCCCAGCCAGCGCCTCCAGGCCCAGCTGAGGAGCTGTGAGAAGCAGCAGAGGGGACTCCCCATCCCGGGCACACCCTG TCCTCCCACCCCTGCCCCCTTGCCCTTCCAGCCCTTTCAGCTGCAGCTGGGAGCTGGCCCGTCAAGTGCTGCCCCTGCCTGTGTCTGGGTTTCTGTTGGC TGTTTTTCTTTTCTTGAGTGGTGATTTTTCTCTAAATAAAAGAAGTCAAGCACTGA

refseq_OTOF.F1 (forward primer)
refseq_OTOF.R1 (reverse primer)
Single exon skipping

Frame 0

IGGGSGGGGGGSGREERGV*AVLHRLAPSRCSC LAVPGSPHQHGLAHPPQDSLGAAGQGRPDRQSDF PRAILLLSGPGEL*GCG*L**DISVAGGQQHRQ K*DAGDSGFQLQQSLQQQAHRDLPHGAAEGGRG EPCGGD*HAD**QQCYHQDQPVRGGPVSGH*RHS GLLGRWGLPGR*VSSRGREGQPRDGWTAPRLPA QLPAPRREELPESREERVLRHEARQKPVSQGGA PKTR*TGGAGDGRP*PSGHSARRWTGSRLGVSSL SHSSHH*CLQQAI*ARH*DGAKCWAAHGLPGQH HGDRGPAAGGLEHGPCGVRGGG*RQEVHIHEGV H*LPLLQRVLRLRLPCLSGCHV*QDHQDFGDSLQ EPAAQWHPGGLLQNGRGNRVLAARAPVPSQVGH PV*PR*HLLGAEGLREV*RCRGGQRGQHQDAPQ GQ*DRRR*H*GELAAPRGGAPRTPVGPVLCENLP SRGAAPYEHKPHGQCKEGFHR*KQGPRGPLRAS LLCWPEGQDFSAEEQL*APVE*AGRLYRPLPPT LQTHEGADPRLGQGQRRGHRHPLH*PAQDF**RR QRLPAHTGPSLGEHVRLHT*LHAAG*ASGPERG PGGGCVLPGPAPAGPGCGDRRHLQP*AHQLHRG AGGAGHAHLGELCR*NGRILSLWSLPGGLNDRPE KRRQAHHL*GHHRQLWERS*WPVPAPAASAPEG AGG*GRSRPDSERK**RGR*CRGPGLSLLHSTN AAPGHRQELLPSALPGAKALHLHQELVAGPAPPP LQCQHHGPHCRQAGRRPERHTGDDQNGEVLP*A SPAGRPGGAELWLLPLPLPR*QGPGPLIPHQA* PGAPQVLHEGAGKHGAAGQDAAGPGEAAHGAGQA EAVPELPAEAALPGGRAPAQHSRHLHLDDEQQQ ACRLCPCALQGPALLHRGGGDWQGLRQGQDALP *AAREAGLRLGRLDSAGQGGAVPVAGPQQTAQGV PVRPALWLPGGQGSPGPGPACLPTRQPGLHQEA GVPAPSAHVPGPQPLCRRQQRTLRPLCPRLLHQ SESVHRGAE*DPVSHLGPDAGVRQPGALW*SS*A EGRSAHHCH*NL*PGFHGQS*LHGPDLRQTPGE DGRRGVLPTPLPTSARVLPDLPWQRHSWRPAGG LRAAADWTSREG*PAPHQWPGGRGPRSHHARAHG HPARAQQVPSGGAVLGPTGPKAGEPGPGGPATG GHRVCREGGAVVPDPQL*EEPQLQHPRQVV*SG PPRERAAAPALEHPCGGLPGLRSLHTGGLPCRQL PATLHLPAPRPLGPQLEHHGQASPALPCAVQWG LLLSLHRGGCGDYGARGTHQETGDHGEAGRDF* SCCQGGCG*GGEGEEEEEEGHCGGARGGGARREH AGLVVQVLCLH*HHEGATSTTRALWN*LGGEGG SGQYRGPEGVNEGQGEGKGCQRGEEEENSELWL WPGVRGPREEETQD**A*GIPQRAGVRV**L*GL AAHFQLASGQDRG**GWLHRGGAHCGTLQGLPL RVQSATPRGRVPGSRLRLHLRHVPGHPEQ*PHQ CAGPSLCGPGHGPAPC*HQRQS*PLHRHPARQD* HPRQGELHLQAAQPCLWEVL*HRGLLPHGIHAD GGCV*LGPGGH**PHWGNQDRPGEPLLQQAPRH LRHRPDLLHTWLQYLAGPHEAQPDPDPPLQRRQS GRPPLWAPWESEGGQPRLHWAL*D*GRERSEEA HRRACGAVGPEALGGHPPRRLPPGARACGDEAA AQPRQAGHRAGPPGAVGGHVPHGHASPWDASGHL TSEAQEVRAAGHHLEHR*GGLGGRRLLHRGEVQ *HLREGVAEGPAGGQAGHRRPLPLPHWRGQLQL ALPVPLRLPGGGGEDRHLQEGVHVLLGRDRVQDP RAAHPADLGCGPLLR*RLPGGHRAGPEPVPAGR KDSQAVHHGDGHRGGGRAPRVHLQAKARQRLVA PPGPQ*ER*V*AHGQGGG*AAFTDSRGGREEPSG PGPQ*T*PPRETQPARHELHLVPEPSQVGSLLL VAHVSLAAPQTVAAPAAAPPPRPVPLLCAWLPG QENPRGLSPVASWPARHGLRLVPQPSQVHQVPHL HPVQVAHHQDRAGAVGAAHVGALPLQPPWLHGQ KAPWGMKAASSRQPLPSPAAFPFSGLDSFPSPL GSLRSPASTLHALGRACLLLAGAACPHCPRLRLA QSRPSDPCLWAGEPWMGWGPGMGLSCPTWLRRH PSSGPGSRGRLLKPSPGLPSTGLKL**N*PRTM GQCPGL*KE*TSKRYPRRDPCR*HRSLIRSRGQT LPTSPSAAASATAGLHLAPLTSQGVACPACGFP WLPRAPSAPASASRPS*GAVRSSRGDSPSRAHP VLPPLPPCPSSPFSCSWELARQVLPLPVSGFLLA VFLFLSGDFSLNKRSQAL

Frame 1

SEGGREEEEEAAAEKREACEPCSTG*LLPAAPA WQCQAAHTSMALLIHLKTVSELRGRGDRIAKVT FRGQSFYSRVLENCEDVADFDETFRWPVASSIDR NEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEE SHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGT VGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRP SSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEP QRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLA SVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSI TVIEARQLVGLNMDPVVCVEVGDDKKYTSMKES TNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHS KNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAI LSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHK ANETDEDDIEGNLLLPEGVPPERQWARFYVKIY RAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQV FFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPL CKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDG DKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEG LGEGVSFRARLLLGLAVEIVDTSNPELTSSTEV QVEQATPISESCAGKMEEFFLFGAFLEASMIDR RNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKE PGDEEEVDLIQNASDDEAGDAGDLASVSSTPPM RPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRR LYNANIMDHIADKLEEGLNDIQEMIKTEKSYPER RLRGVLEELSCGCCRFLSLADKDQGHSSRTRLD RERLKSCMRELENMGQQARMLRAQVKRHTVRDK LRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNK RVAYARVPSKDLLFSIVEEETGKDCAKVKTLFL KLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKE FLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQ AFQLRAHMYQARSLFAADSSGLSDPFARVFFIN QSQCTEVLNETLCPTWDQMLVFDNLELYGEAHE LRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVK MADEAYCPPRFPPQLEYYQIYRGNATAGDLLAA FELLQIGPAGKADLPPINGPVDVDRGPIMPVPM GIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRV DIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVD LPENELLHPPLNIRVVDCRAFGRYTLVGSHAVS SLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGG SSSHSTGEVVVTMEPEVPIKKLETMVKLDATSE AVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDES MLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEE VDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGS GQGSEAPEKKKPKIDELKVYPKELESEFDNFED WLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLC VYKVPLPEDVSREAGYDSTYGMFQGIPSNDPIN VLVRVYVVRATDLHPADINGKADPYIAIRLGKT DIRDKENYISKQLNPVFGKSFDIEASFPMESMLT VAVYDWDLVGTDDLIGETKIDLENRFYSKHRAT CGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGK VDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKP TDEHVALLALRHWEDIPRAGCRLVPEHVETRPL LNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDI SPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSS DIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNW RYLFPFDYLAAEEKIVISKKESMFSWDETEYKI PARLTLQIWDADHFSADDFLGAIELDLNRFPRGA KTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWP LLARNENDEFELTGKVEAELHLLTAEEAEKNPV GLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFL WHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLV KKILGA*AQWPPGRPDTAFVWFLNPLKSIKYLI CTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVK KLLGA*RPPAPASRSPALPHFLSVAWTLSHLPW GA*GAQRPLFMPWAEPASCLRGPPVLTAPGCGL PSPAPLTPACGLGSLGWGGDLEWVSLAPPG*GAT LLQAQAPEEDS*NPPQVFQVQD*SFSEINQGPW VSAQGFKKNERAKGIPAVTPADSTGL*SAAGAR PCPQVPARLLLPLLGSTWLLSLPRGSPVLPVGFH GFPELPLPQPAPPGPAEEL*EAAEGTPHPGHTL SSHPCPLALPALSAAAGSWPVKCCPCLCLGFCW LFFFS*VVIFL*IKEVKH*

Frame 2

RRGVGRRRRRQRQRRERRVSRAPPASSFPLLLP GSARQPTPAWPCSSTSRQSRSCGAGATGSPK*L SEGNPSTLGSWRTVRMWLTLMRHFGGRWPAAST EMRCWRFRFSTTAKSSATSSSGPSAWCCRRW*RR AMWR*LTR*LMTTMLSSRPACAWRSGIRPLTAQ WAPGTMGTSWEMSLFKRKRRTAKRRMDCSQAPG PAPGPQERRASGEPGGACSPP*SSAKTGLTRRSP KDQMNRRCWRWKTLTIWPFG*EMDWIPTRCL*P QSQLSPLMSPTSDLSQTLRWSQVLGGPWITRSA SR*SRPGSWWA*TWTLWCAWRWVTTRSTHP*RSP LTAPITTSTSSSTSMSLRMSCLTRSSRFR*FTP RTCCAVAPWWAPSKWTWEPCTRSQSTSSITSGP SCLTPMTSPRG*RAT*SVTLPWWAKGTTSRRPTR PMRPTKMTLRGTCCSPRGCPPNASGPGSM*KFT EQRGCPV*TQASWPM*RRLSSVKTRTSWTPTCK SSLLARRARLQCRRAAMSPCGMSRSSLQTSSPHS ANA*RCRSETRTRSTTWPSAPTSLTCARFLMTE TKASCPHWAQPG*TCTAPHVTTRCWMSIRT*TR AWGRVCPSGPGSCWAWLWRS*TPPTLSSPAPQRC RWSRPRPSRRAVQVKWKNSFSLEPSWRPQ*STG ETETSPSPLRSP*ATMGTKLMACPGPSGLGPGR SRGMRKK*T*FRTQVMTRPVMPGTWPQSPPLHQC GPRSPTGTTSICPTWSESPASTSRAGGRTSAAA STMPTSWTTLPTSWKKA*TTYRR*SKRRSPTLS VACGASWRS*AVAAAASSPSLTRTRATHPAPGLT GSASSPA*GSWKTWGSRPGCCGPR*SGTRCGTS *GCARTSCRSCASWRTSPSTAFPTSSSG**ATT SVSPMPVCPPRTCSSPSWRRRLARTAPRSRRSSL SCQGSGASARQAGQCRPRWSCTCGWASANSARS SCAACPVASRRSRQPRAWACMPSHPSAWSTPRS RRSSSERTCTRPAASLPPTAADSQTPLPASSSSI RVSAQRC*MRPCVPPGTRCWCSTTWSSMVKLMS *GTIRPSLSLKSMTRIPWAKLTSWAGPSPNPW* RWQTRRTAHPASHLSSSTTRSTVATPQLETCWRP SSCCRLDQQGRLTCPPSMARWTWTEVPSCPCPW ASGPCSASTEWRCCSGAYGT*SG*TWPRWTGHG WTSSVQGRGCSRP*STIIRRTPTSTPSSSGLKWT SQRTSCCTRP*TSVWWTAGPSVATHWWAPMPSA PCDASSTGPQTARPPAGTPRSGFSGAAVCCAMG APPLTPQGRLW*LWSQRYPSRNWRPW*SWTRLLK LLSRWMWLRRRRRRRRRRRALRRSQRRRSQTRA CWTGGPSTLPPLTP*RSNFDNKSPLELTWRRRR KWTIPRA*RGQ*RARRRQGLPKRRRRRKLRALAL ARGPRPPRRRNPRLMSLRYTPKSWSPSLITLRT GCTLSTCFGARPGMMRMAPPRRSALWDASRAPS ACTKCHSQRTCPGKPATTPPTACSRASRAMTPSM CWSESMWSGPRTCTLLTSTAKLTPTSPSG*ARL TSATRRTTSPSSSTLSLGSPLTSRPPSPWNPC* RWLCMTGTWWALMTSLGKPRSTWRTASTASTAPP AASPRPTPHMATISGGTP*SPARS*PASAKTAK WTAPTLGPLGE*RWPTASSLGPLRLRTRTVRGS PQTSMWRCWP*GTGRTSPAQAAAWCQSMWRRGRC STPTSRASSRAAWSCGWTCSPWTCQPLGRLWTS HLGSPRSTSCGSSSGTQMRWSWRTTTSSQGRSP VTSS*GGG*RASRRTSRTQTSTTTPSLARATSTG ATCSPSTTWRRRRRSSSPRRSPCSPGTRPSTRS PRGSPCRSGMRTTSPLTTSWGPSSWT*TGSRGA QRQPSSAPWRWPPGRWTCPSCPSSSKSASKAGGP SWPAMRTMSLSSRARWRLSCIY*QQRRQRRTQW AWPAMNLTP*RNPTGPTRASSGS*TLSSRLATS CGTRIAGCSSNCCCSCCCSSSSPCSSTLCLATWS RKSSGPEPSGLLAGPTRPSSGSSTLSSPSSTSS APGTSGSSSRSCWRCWGCSCWGSSSTASLATWS KSSLGHEGRQLPPAAPQPCRISFQWLGLFPISPG EPEEPSVHSSCLGPSLPPACGGRLSSLPQAAAC PVPPL*PLPVGWGALDGVGTWNGSLLPHLAEAP PFFRPRLQRKTPETLPRSSKYRIEALVKLTKDHG SVPRALKRMNEQKVSPP*PLQIAPVFDPQQGPD PAHKSQRGCFCHCWAPLGSSHFPGGRLSCLWVS MASQSSLCPSQRLQAQLRSCEKQQRGLPIPGTPC PPTPAPLPFQPFQLQLGAGPSSAAPACVWVSVG CFSFLEW*FFSK*KKSST

Sequence NM_004802

CCGTGAGTTCTGCCCAGGCCCTGTGAGCTCACCAGAGCCACAGACTCACAGCCCAGAGGTGGCTTCTTCCTTCAGGAACTGAAGAACCCCCATGAACACC AACATCTCCAGGTTCTGAGAACAGAACCTGGGAAATTGATGACTTCCTCATGATGACCGATACTCAGGATGGCCCTAGCGAGAGCTCCCAGATCATGAGG AAGAAGGCCTGAACGACATACAGGAGATGATCAAAACGGAGAAGTCCTACCCTGAGCGTCGCCTGCGGGGCGTCCTGGAGGAGCTGAGCTGTGGCTGCTG CCGCTTCCTCTCCCTCGCTGACAAGGACCAGGGCCACTCATCCCGCACCAGGCTTGACCGGGAGCGCCTCAAGTCCTGCATGAGGGAGCTGGAAAACATG GGGCAGCAGGCCAGGATGCTGCGGGCCCAGGTGAAGCGGCACACGGTGCGGGACAAGCTGAGGCTGTGCCAGAACTTCCTGCAGAAGCTGCGCTTCCTGG CGGACGAGCCCCAGCACAGCATTCCCGACATCTTCATCTGGATGATGAGCAACAACAAGCGTGTCGCCTATGCCCGTGTGCCCTCCAAGGACCTGCTCTT CTCCATCGTGGAGGAGGAGACTGGCAAGGACTGCGCCAAGGTCAAGACGCTCTTCCTTAAGCTGCCAGGGAAGCGGGGCTTCGGCTCGGCAGGCTGGACA GTGCAGGCCAAGGTGGAGCTGTACCTGTGGCTGGGCCTCAGCAAACAGCGCAAGGAGTTCCTGTGCGGCCTGCCCTGTGGCTTCCAGGAGGTCAAGGCAG CCCAGGGCCTGGGCCTGCATGCCTTCCCACCCGTCAGCCTGGTCTACACCAAGAAGCAGGCGTTCCAGCTCCGAGCGCACATGTACCAGGCCCGCAGCCT CTTTGCCGCCGACAGCAGCGGACTCTCAGACCCCTTTGCCCGCGTCTTCTTCATCAATCAGAGTCAGTGCACAGAGGTGCTGAATGAGACCCTGTGTCCC ACCTGGGACCAGATGCTGGTGTTCGACAACCTGGAGCTCTATGGTGAAGCTCATGAGCTGAGGGACGATCCGCCCATCATTGTCATTGAAATCTATGACC AGGATTCCATGGGCAAAGCTGACTTCATGGGCCGGACCTTCGCCAAACCCCTGGTGAAGATGGCAGACGAGGCGTACTGCCCACCCCGCTTCCCACCTCA GCTCGAGTACTACCAGATCTACCGTGGCAACGCCACAGCTGGAGACCTGCTGGCGGCCTTCGAGCTGCTGCAGATTGGACCAGCAGGGAAGGCTGACCTG CCCCCCATCAATGGCCCGGTGGACGTGGACCGAGGTCCCATCATGCCCGTGCCCATGGGCATCCGGCCCGTGCTCAGCAAGTACCGAGTGGAGGTGCTGT TCTGGGGCCTACGGGACCTAAAGCGGGTGAACCTGGCCCAGGTGGACCGGCCACGGGTGGACATCGAGTGTGCAGGGAAGGGGGTGCAGTCGTCCCTGAT CCACAATTATAAGAAGAACCCCAACTTCAACACCCTCGTCAAGTGGTTTGAAGTGGACCTCCCAGAGAACGAGCTGCTGCACCCGCCCTTGAACATCCGT GTGGTGGACTGCCGGGCCTTCGGTCGCTACACACTGGTGGGCTCCCATGCCGTCAGCTCCCTGCGACGCTTCATCTACCGGCCCCCAGACCGCTCGGCCC CCAGCTGGAACACCACGGGGGAGGTTGTGGTGACTATGGAGCCAGAGGTACCCATCAAGAAACTGGAGACCATGGTGAAGCTGGACGCGACTTCTGAAGC TGTTGTCAAGGTGGATGTGGCTGAGGAGGAGAAGGAGAAGAAGAAGAAGAAGAAGGGCACTGCGGAGGAGCCAGAGGAGGAGGAGCCAGACGAGAGCATG CTGGACTGGTGGTCCAAGTACTTTGCCTCCATTGACACCATGAAGGAGCAACTTCGACAACAAGAGCCCTCTGGAATTGACTTGGAGGAGAAGGAGGAAG TGGACAATACCGAGGGCCTGAAGGGGTCAATGAAGGGCAAGGAGAAGGCAAGGGCTGCCAAAGAGGAGAAGAAGAAGAAAACTCAGAGCTCTGGCTCTGG CCAGGGGTCCGAGGCCCCCGAGAAGAAGAAACCCAAGATTGATGAGCTTAAGGTATACCCCAAAGAGCTGGAGTCCGAGTTTGATAACTTTGAGGACTGG CTGCACACTTTCAACTTGCTTCGGGGCAAGACCGGGGATGATGAGGATGGCTCCACCGAGGAGGAGCGCATTGTGGGACGCTTCAAGGGCTCCCTCTGCG TGTACAAAGTGCCACTCCCAGAGGACGTGTCCCGGGAAGCCGGCTACGACTCCACCTACGGCATGTTCCAGGGCATCCCGAGCAATGACCCCATCAATGT GCTGGTCCGAGTCTATGTGGTCCGGGCCACGGACCTGCACCCTGCTGACATCAACGGCAAAGCTGACCCCTACATCGCCATCCGGCTAGGCAAGACTGAC ATCCGCGACAAGGAGAACTACATCTCCAAGCAGCTCAACCCTGTCTTTGGGAAGTCCTTTGACATCGAGGCCTCCTTCCCCATGGAATCCATGCTGACGG TGGCTGTGTATGACTGGGACCTGGTGGGCACTGATGACCTCATTGGGGAAACCAAGATCGACCTGGAGAACCGCTTCTACAGCAAGCACCGCGCCACCTG CGGCATCGCCCAGACCTACTCCACACATGGCTACAATATCTGGCGGGACCCCATGAAGCCCAGCCAGATCCTGACCCGCCTCTGCAAAGACGGCAAAGTG GACGGCCCCCACTTTGGGCCCCCTGGGAGAGTGAAGGTGGCCAACCGCGTCTTCACTGGGCCCTCTGAGATTGAGGACGAGAACGGTCAGAGGAAGCCCA CAGACGAGCATGTGGCGCTGTTGGCCCTGAGGCACTGGGAGGACATCCCCCGCGCAGGCTGCCGCCTGGTGCCAGAGCATGTGGAGACGAGGCCGCTGCT CAACCCCGACAAGCCGGGCATCGAGCAGGGCCGCCTGGAGCTGTGGGTGGACATGTTCCCCATGGACATGCCAGCCCCTGGGACGCCTCTGGACATCTCA CCTCGGAAGCCCAAGAAGTACGAGCTGCGGGTCATCATCTGGAACACAGATGAGGTGGTCTTGGAGGACGACGACTTCTTCACAGGGGAGAAGTCCAGTG ACATCTTCGTGAGGGGGTGGCTGAAGGGCCAGCAGGAGGACAAGCAGGACACAGACGTCCACTACCACTCCCTCACTGGCGAGGGCAACTTCAACTGGCG CTACCTGTTCCCCTTCGACTACCTGGCGGCGGAGGAGAAGATCGTCATCTCCAAGAAGGAGTCCATGTTCTCCTGGGACGAGACCGAGTACAAGATCCCC GCGCGGCTCACCCTGCAGATCTGGGATGCGGACCACTTCTCCGCTGACGACTTCCTGGGGGCCATCGAGCTGGACCTGAACCGGTTCCCGCGGGGCGCAA AGACAGCCAAGCAGTGCACCATGGAGATGGCCACCGGGGAGGTGGACGTGCCCCTCGTGTCCATCTTCAAGCAAAAGCGCGTCAAAGGCTGGTGGCCCCT CCTGGCCCGCAATGAGAACGATGAGTTTGAGCTCACGGGCAAGGTGGAGGCTGAGCTGCATTTACTGACAGCAGAGGAGGCAGAGAAGAACCCAGTGGGC CTGGCCCGCAATGAACCTGACCCCCTAGAGAAACCCAACCGGCCCGACACGAGCTTCATCTGGTTCCTGAACCCTCTCAAGTCGGCTCGCTACTTCTTGT GGCACACGTATCGCTGGCTGCTCCTCAAACTGTTGCTGCTCCTGCTGCTGCTCCTCCTCCTCGCCCTGTTCCTCTACTCTGTGCCTGGCTACCTGGTCAA GAAAATCCTCGGGGCCTGAGCCCAGTGGCCTCCTGGCCGGCCCGACACGGCCTTCGTCTGGTTCCTCAACCCTCTCAAGTCCATCAAGTACCTCATCTGC ACCCGGTACAAGTGGCTCATCATCAAGATCGTGCTGGCGCTGTTGGGGCTGCTCATGTTGGGGCTCTTCCTCTACAGCCTCCCTGGCTACATGGTCAAAA AGCTCCTTGGGGCATGAAGGCCGCCAGCTCCCGCCAGCCGCTCCCCAGCCCTGCCGCATTTCCTTTCAGTGGCTTGGACTCTTTCCCATCTCCCCTGGGG AGCCTGAGGAGCCCAGCGTCCACTCTTCATGCCTTGGGCCGAGCCTGCCTCCTGCTTGCGGGGGCCGCCTGTCCTCACTGCCCCAGGCTGCGGCTTGCCC AGTCCCGCCCCTCTGACCCCTGCCTGTGGGCTGGGGAGCCTTGGATGGGGTGGGGACCTGGAATGGGTCTCTCTTGCCCCACCTGGCTGAGGCGCCACCC TTCTTCAGGCCCAGGCTCCAGAGGAAGACTCCTGAAACCCTCCCCAGGTCTTCCAAGTACAGGATTGAAGCTTTAGTGAAATTAACCAAGGACCATGGGT CAGTGCCCAGGGCTTTAAAAAGAATGAACGAGCAAAAGGTATCCCCGCCGTGACCCCTGCAGATAGCACCGGTCTTTGATCCGCAGCAGGGGCCAGACCC TGCCCACAAGTCCCAGCGCGGCTGCTTCTGCCACTGCTGGGCTCCACTTGGCTCCTCTCACTTCCCAGGGGGTCGCCTGTCCTGCCTGTGGGTTTCCATG GCTTCCCAGAGCTCCCTCTGCCCCAGCCAGCGCCTCCAGGCCCAGCTGAGGAGCTGTGAGAAGCAGCAGAGGGGACTCCCCATCCCGGGCACACCCTGTC CTCCCACCCCTGCCCCCTTGCCCTTCCAGCCCTTTCAGCTGCAGCTGGGAGCTGGCCCGTCAAGTGCTGCCCCTGCCTGTGTCTGGGTTTCTGTTGGCTG TTTTTCTTTTCTTGAGTGGTGATTTTTCTCTAAATAAAAGAAGTCAAGCACTGA

refseq_OTOF.F1 (forward primer)
refseq_OTOF.R1 (reverse primer)
Single exon skipping

Frame 0

P*VLPRPCELTRATDSQPRGGFFLQELKNPHEH QHLQVLRTEPGKLMTSS**PILRMALARAPRS*G RRPERHTGDDQNGEVLP*ASPAGRPGGAELWLL PLPLPR*QGPGPLIPHQA*PGAPQVLHEGAGKH GAAGQDAAGPGEAAHGAGQAEAVPELPAEAALPG GRAPAQHSRHLHLDDEQQQACRLCPCALQGPAL LHRGGGDWQGLRQGQDALP*AAREAGLRLGRLD SAGQGGAVPVAGPQQTAQGVPVRPALWLPGGQGS PGPGPACLPTRQPGLHQEAGVPAPSAHVPGPQP LCRRQQRTLRPLCPRLLHQSESVHRGAE*DPVS HLGPDAGVRQPGALW*SS*AEGRSAHHCH*NL*P GFHGQS*LHGPDLRQTPGEDGRRGVLPTPLPTS ARVLPDLPWQRHSWRPAGGLRAAADWTSREG*P APHQWPGGRGPRSHHARAHGHPARAQQVPSGGAV LGPTGPKAGEPGPGGPATGGHRVCREGGAVVPD PQL*EEPQLQHPRQVV*SGPPRERAAAPALEHP CGGLPGLRSLHTGGLPCRQLPATLHLPAPRPLGP QLEHHGGGCGDYGARGTHQETGDHGEAGRDF*S CCQGGCG*GGEGEEEEEEGHCGGARGGGARREH AGLVVQVLCLH*HHEGATSTTRALWN*LGGEGGS GQYRGPEGVNEGQGEGKGCQRGEEEENSELWLW PGVRGPREEETQD**A*GIPQRAGVRV**L*GL AAHFQLASGQDRG**GWLHRGGAHCGTLQGLPLR VQSATPRGRVPGSRLRLHLRHVPGHPEQ*PHQC AGPSLCGPGHGPAPC*HQRQS*PLHRHPARQD* HPRQGELHLQAAQPCLWEVL*HRGLLPHGIHADG GCV*LGPGGH**PHWGNQDRPGEPLLQQAPRHL RHRPDLLHTWLQYLAGPHEAQPDPDPPLQRRQS GRPPLWAPWESEGGQPRLHWAL*D*GRERSEEAH RRACGAVGPEALGGHPPRRLPPGARACGDEAAA QPRQAGHRAGPPGAVGGHVPHGHASPWDASGHL TSEAQEVRAAGHHLEHR*GGLGGRRLLHRGEVQ* HLREGVAEGPAGGQAGHRRPLPLPHWRGQLQLA LPVPLRLPGGGGEDRHLQEGVHVLLGRDRVQDP RAAHPADLGCGPLLR*RLPGGHRAGPEPVPAGRK DSQAVHHGDGHRGGGRAPRVHLQAKARQRLVAP PGPQ*ER*V*AHGQGGG*AAFTDSRGGREEPSG PGPQ*T*PPRETQPARHELHLVPEPSQVGSLLLV AHVSLAAPQTVAAPAAAPPPRPVPLLCAWLPGQ ENPRGLSPVASWPARHGLRLVPQPSQVHQVPHL HPVQVAHHQDRAGAVGAAHVGALPLQPPWLHGQK APWGMKAASSRQPLPSPAAFPFSGLDSFPSPLG SLRSPASTLHALGRACLLLAGAACPHCPRLRLA QSRPSDPCLWAGEPWMGWGPGMGLSCPTWLRRHP SSGPGSRGRLLKPSPGLPSTGLKL**N*PRTMG QCPGL*KE*TSKRYPRRDPCR*HRSLIRSRGQT LPTSPSAAASATAGLHLAPLTSQGVACPACGFPW LPRAPSAPASASRPS*GAVRSSRGDSPSRAHPV LPPLPPCPSSPFSCSWELARQVLPLPVSGFLLA VFLFLSGDFSLNKRSQAL

Frame 1

REFCPGPVSSPEPQTHSPEVASSFRN*RTPMNT NISRF*EQNLGN**LPHDDRYSGWP*RELPDHE EEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCC RFLSLADKDQGHSSRTRLDRERLKSCMRELENM GQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFL ADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLF SIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWT VQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKA AQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSL FAADSSGLSDPFARVFFINQSQCTEVLNETLCP TWDQMLVFDNLELYGEAHELRDDPPIIVIEIYD QDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQ LEYYQIYRGNATAGDLLAAFELLQIGPAGKADL PPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVL FWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLI HNYKKNPNFNTLVKWFEVDLPENELLHPPLNIR VVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSA PSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEA VVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESM LDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEE VDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSG QGSEAPEKKKPKIDELKVYPKELESEFDNFEDW LHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLC VYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINV LVRVYVVRATDLHPADINGKADPYIAIRLGKTD IRDKENYISKQLNPVFGKSFDIEASFPMESMLT VAVYDWDLVGTDDLIGETKIDLENRFYSKHRATC GIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKV DGPHFGPPGRVKVANRVFTGPSEIEDENGQRKP TDEHVALLALRHWEDIPRAGCRLVPEHVETRPLL NPDKPGIEQGRLELWVDMFPMDMPAPGTPLDIS PRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSS DIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWR YLFPFDYLAAEEKIVISKKESMFSWDETEYKIP ARLTLQIWDADHFSADDFLGAIELDLNRFPRGA KTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPL LARNENDEFELTGKVEAELHLLTAEEAEKNPVG LARNEPDPLEKPNRPDTSFIWFLNPLKSARYFL WHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVK KILGA*AQWPPGRPDTAFVWFLNPLKSIKYLIC TRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVK KLLGA*RPPAPASRSPALPHFLSVAWTLSHLPWG A*GAQRPLFMPWAEPASCLRGPPVLTAPGCGLP SPAPLTPACGLGSLGWGGDLEWVSLAPPG*GAT LLQAQAPEEDS*NPPQVFQVQD*SFSEINQGPWV SAQGFKKNERAKGIPAVTPADSTGL*SAAGARP CPQVPARLLLPLLGSTWLLSLPRGSPVLPVGFH GFPELPLPQPAPPGPAEEL*EAAEGTPHPGHTLS SHPCPLALPALSAAAGSWPVKCCPCLCLGFCWL FFFS*VVIFL*IKEVKH*

Frame 2

VSSAQAL*AHQSHRLTAQRWLLPSGTEEPP*TP TSPGSENRTWEIDDFLMMTDTQDGPSESSQIMR KKA*TTYRR*SKRRSPTLSVACGASWRS*AVAA AASSPSLTRTRATHPAPGLTGSASSPA*GSWKTW GSRPGCCGPR*SGTRCGTS*GCARTSCRSCASW RTSPSTAFPTSSSG**ATTSVSPMPVCPPRTCS SPSWRRRLARTAPRSRRSSLSCQGSGASARQAGQ CRPRWSCTCGWASANSARSSCAACPVASRRSRQ PRAWACMPSHPSAWSTPRSRRSSSERTCTRPAA SLPPTAADSQTPLPASSSSIRVSAQRC*MRPCVP PGTRCWCSTTWSSMVKLMS*GTIRPSLSLKSMT RIPWAKLTSWAGPSPNPW*RWQTRRTAHPASHL SSSTTRSTVATPQLETCWRPSSCCRLDQQGRLTC PPSMARWTWTEVPSCPCPWASGPCSASTEWRCC SGAYGT*SG*TWPRWTGHGWTSSVQGRGCSRP* STIIRRTPTSTPSSSGLKWTSQRTSCCTRP*TSV WWTAGPSVATHWWAPMPSAPCDASSTGPQTARP PAGTPRGRLW*LWSQRYPSRNWRPW*SWTRLLK LLSRWMWLRRRRRRRRRRRALRRSQRRRSQTRAC WTGGPSTLPPLTP*RSNFDNKSPLELTWRRRRK WTIPRA*RGQ*RARRRQGLPKRRRRRKLRALAL ARGPRPPRRRNPRLMSLRYTPKSWSPSLITLRTG CTLSTCFGARPGMMRMAPPRRSALWDASRAPSA CTKCHSQRTCPGKPATTPPTACSRASRAMTPSM CWSESMWSGPRTCTLLTSTAKLTPTSPSG*ARLT SATRRTTSPSSSTLSLGSPLTSRPPSPWNPC*R WLCMTGTWWALMTSLGKPRSTWRTASTASTAPP AASPRPTPHMATISGGTP*SPARS*PASAKTAKW TAPTLGPLGE*RWPTASSLGPLRLRTRTVRGSP QTSMWRCWP*GTGRTSPAQAAAWCQSMWRRGRC STPTSRASSRAAWSCGWTCSPWTCQPLGRLWTSH LGSPRSTSCGSSSGTQMRWSWRTTTSSQGRSPV TSS*GGG*RASRRTSRTQTSTTTPSLARATSTG ATCSPSTTWRRRRRSSSPRRSPCSPGTRPSTRSP RGSPCRSGMRTTSPLTTSWGPSSWT*TGSRGAQ RQPSSAPWRWPPGRWTCPSCPSSSKSASKAGGP SWPAMRTMSLSSRARWRLSCIY*QQRRQRRTQWA WPAMNLTP*RNPTGPTRASSGS*TLSSRLATSC GTRIAGCSSNCCCSCCCSSSSPCSSTLCLATWS RKSSGPEPSGLLAGPTRPSSGSSTLSSPSSTSSA PGTSGSSSRSCWRCWGCSCWGSSSTASLATWSK SSLGHEGRQLPPAAPQPCRISFQWLGLFPISPG EPEEPSVHSSCLGPSLPPACGGRLSSLPQAAACP VPPL*PLPVGWGALDGVGTWNGSLLPHLAEAPP FFRPRLQRKTPETLPRSSKYRIEALVKLTKDHG SVPRALKRMNEQKVSPP*PLQIAPVFDPQQGPDP AHKSQRGCFCHCWAPLGSSHFPGGRLSCLWVSM ASQSSLCPSQRLQAQLRSCEKQQRGLPIPGTPC PPTPAPLPFQPFQLQLGAGPSSAAPACVWVSVGC FSFLEW*FFSK*KKSST