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Sequences

Sequence NM_153427

TGGGAGTCCGTGCTCCTGCTCCTCGGTTGGCTCCTAAGTGCCCCGCCAGGTCCCCTCTCCTTTCGCTCTCCCGGCTCCGGCTCCCGACTCTTCGGCCCGC
TGGCATCTGCTTCCCTCCCCTGCCTCGTTTCTCGTCGCCCCTGCTCGCTCCCCCCGGCGCTCGCCCGGGCGCTGTGCTCGCTCCTGGATCGCCAGCCGCG
CAGCCGGGCTCGGCCGGCCGCCCGCGCGCCACTGTGCAGTGGAGTTTGGTGGAATCTCTGCTGACGTCACGTCACTCCCCACACGGAGTAGGAGCAGAGG
GAAGAGAGAGGGATGAGAGGGAGGGAGAGGAGAGAGAGTGCGAGACCGAGCGAGAAAGCTGGAGAGGAGCAGAAAGAAACTGCCAGTGGCGGCTAGATTT
CGGAGGCCCCAGTGCACCCGTGGACTCCTTCGGAACTTGGCACCCTCAGGAGCCCTGCAGTCCTCTCAGGCCCGGCTTTCGGGCGCTTGCCGTGCAGCCG
GAGGCTCGGCTCGCTGGAAATCGCCCCGGGAAGCAGTGGGACGCGGAGACAGCAGCTCTCTCCCGGTAGCCGATAACGGGGAATGGAGACCAACTGCCGC
AAACTGGTGTCGGCGTGTGTGCAATTAGAGAAAGATAAAAGCCAGCAGGGGAAGAATGAGGACGTGGGCGCCGAGGACCCGTCTAAGAAGAAGCGGCAAA
GGCGGCAGCGGACTCACTTTACCAGCCAGCAGCTCCAGGAGCTGGAGGCCACTTTCCAGAGGAACCGCTACCCGGACATGTCCACACGCGAAGAAATCGC
TGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGTCGGGCCAAATGGAGAAAGAGGGAGCGCAACCAGCAGGCCGAGCTATGC
AAGAATGGCTTCGGGCCGCAGTTCAATGGGCTCATGCAGCCCTACGACGACATGTACCCAGGCTATTCCTACAACAACTGGGCCGCCAAGGGCCTTACAT
CCGCCTCCCTATCCACCAAGAGCTTCCCCTTCTTCAACTCTATGAACGTCAACCCCCTGTCATCACAGAGCATGTTTTCCCCACCCAACTCTATCTCGTC
CATGAGCATGTCGTCCAGCATGGTGCCCTCAGCAGTGACAGGCGTCCCGGGCTCCAGTCTCAACAGCCTGAATAACTTGAACAACCTGAGTAGCCCGTCG
CTGAATTCCGCGGTGCCGACGCCTGCCTGTCCTTACGCGCCGCCGACTCCTCCGTATGTTTATAGGGACACGTGTAACTCGAGCCTGGCCAGCCTGAGAC
TGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGCCTCCAACCTGAGTGCTTGCCAGTATGCAGTGGACCGGCCCGTGTGAGC
CGCACCCACAGCGCCGGGATCCTAGGACCTTGCCGGATGGGGCAACTCCGCCCTTGAAAGACTGGGAATTATGCTAGAAGGTCGTGGGCACTAAAGAAAG
GGAGAGAAAGAGAAGCTATATAGAGAAAAGGAAACCACTGAATCAAAGAGAGAGCTCCTTTGATTTCAAAGGGATGTCCTCAGTGTCTGACATCTTTCAC
TACAAGTATTTCTAACAGTTGCAAGGACACATACACAAACAAATGTTTGACTGGATATGACATTTTAACATTACTATAAGCTTGTTATTTTTTAAGTTTA
GCATTGTTAACATTTAAATGACTGAAAGGATGTATATATATCGAAATGTCAAATTAATTTTATAAAAGCAGTTGTTAGTAATATCACAACAGTGTTTTTA
AAGGTTAGGCTTTAAAATAAAGCATGTTATACAGAAGCGATTAGGATTTTTCGCTTGCGAGCAAGGGAGTGTATATACTAAATGCCACACTGTATGTTTC
TAACATATTATTATTATTATAAAAAATGTGTGAATATCAGTTTTAGAATAGTTTCTCTGGTGGATGCAATGATGTTTCTGAAACTGCTATGTACAACCTA
CCCTGTGTATAACATTTCGTACAATATTATTGTTTTACTTTTCAGCAAATATGAAACAAATGTGTTTTATTTCATGGGAGTAAAATATACTGCATACAAA
  • refseq_PITX2.F2 (forward primer)
  • refseq_PITX2.R2 (reverse primer)
  • Single exon skipping

Frame 0

WESVLLLLGWLLSAPPGPLSFRSPGSGSRLFGP
LASASLPCLVSRRPCSLPPALARALCSLLDRQPR
SRARPAARAPLCSGVWWNLC*RHVTPHTE*EQR
EERGMRGRERRESARPSEKAGEEQKETASGG*I
SEAPVHPWTPSELGTLRSPAVLSGPAFGRLPCSR
RLGSLEIAPGSSGTRRQQLSPGSR*RGMETNCR
KLVSACVQLEKDKSQQGKNEDVGAEDPSKKKRQ
RRQRTHFTSQQLQELEATFQRNRYPDMSTREEIA
VWTNLTEARVRVWFKNRRAKWRKRERNQQAELC
KNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLT
SASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISS
MSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPS
LNSAVPTPACPYAPPTPPYVYRDTCNSSLASLR
LKAKQHSSFGYASVQNPASNLSACQYAVDRPV*A
APTAPGS*DLAGWGNSALERLGIMLEGRGH*RK
GEKEKLYREKETTESKRELL*FQRDVLSV*HLS
LQVFLTVARTHTQTNV*LDMTF*HYYKLVIF*V*
HC*HLND*KDVYISKCQINFIKAVVSNITTVFL
KVRL*NKACYTEAIRIFRLRARECIY*MPHCMF
LTYYYYYKKCVNISFRIVSLVDAMMFLKLLCTTY
PVYNISYNIIVLLFSKYETNVFYFMGVKYTAYK

Frame 1

GSPCSCSSVGS*VPRQVPSPFALPAPAPDSSAR
WHLLPSPASFLVAPARSPRRSPGRCARSWIASR
AAGLGRPPARHCAVEFGGISADVTSLPTRSRSRG
KREG*EGGRGERVRDRARKLERSRKKLPVAARF
RRPQCTRGLLRNLAPSGALQSSQARLSGACRAA
GGSARWKSPREAVGRGDSSSLPVADNGEWRPTAA
NWCRRVCN*RKIKASRGRMRTWAPRTRLRRSGK
GGSGLTLPASSSRSWRPLSRGTATRTCPHAKKS
LCGPTLRKPESGFGSRIVGPNGERGSATSRPSYA
RMASGRSSMGSCSPTTTCTQAIPTTTGPPRALH
PPPYPPRASPSSTL*TSTPCHHRACFPHPTLSR
P*ACRPAWCPQQ*QASRAPVSTA*IT*TT*VARR
*IPRCRRLPVLTRRRLLRMFIGTRVTRAWPA*D
*KQSSTPASATPACRTRPPT*VLASMQWTGPCE
PHPQRRDPRTLPDGATPPLKDWELC*KVVGTKER
ERKRSYIEKRKPLNQRESSFDFKGMSSVSDIFH
YKYF*QLQGHIHKQMFDWI*HFNITISLLFFKF
SIVNI*MTERMYIYRNVKLIL*KQLLVISQQCF*
RLGFKIKHVIQKRLGFFACEQGSVYTKCHTVCF
*HIIIIIKNV*ISVLE*FLWWMQ*CF*NCYVQP
TLCITFRTILLFYFSANMKQMCFISWE*NILHT

Frame 2

GVRAPAPRLAPKCPARSPLLSLSRLRLPTLRPA
GICFPPLPRFSSPLLAPPGARPGAVLAPGSPAA
QPGSAGRPRATVQWSLVESLLTSRHSPHGVGAE
GRERDEREGEERECETERESWRGAERNCQWRLDF
GGPSAPVDSFGTWHPQEPCSPLRPGFRALAVQP
EARLAGNRPGKQWDAETAALSR*PITGNGDQLP
QTGVGVCAIRER*KPAGEE*GRGRRGPV*EEAAK
AAADSLYQPAAPGAGGHFPEEPLPGHVHTRRNR
CVDQPYGSPSPGLVQESSGQMEKEGAQPAGRAM
QEWLRAAVQWAHAALRRHVPRLFLQQLGRQGPYI
RLPIHQELPLLQLYERQPPVITEHVFPTQLYLV
HEHVVQHGALSSDRRPGLQSQQPE*LEQPE*PV
AEFRGADACLSLRAADSSVCL*GHV*LEPGQPET
ESKAALQLRLRQRAEPGLQPECLPVCSGPARVS
RTHSAGILGPCRMGQLRP*KTGNYARRSWALKK
GREREAI*RKGNH*IKERAPLISKGCPQCLTSFT
TSISNSCKDTYTNKCLTGYDILTLL*ACYFLSL
ALLTFK*LKGCIYIEMSN*FYKSSC**YHNSVF
KG*ALK*SMLYRSD*DFSLASKGVYILNATLYVS
NILLLL*KMCEYQF*NSFSGGCNDVSETAMYNL
PCV*HFVQYYCFTFQQI*NKCVLFHGSKIYCIQ

Sequence NM_153426

TGGGAGTCCGTGCTCCTGCTCCTCGGTTGGCTCCTAAGTGCCCCGCCAGGTCCCCTCTCCTTTCGCTCTCCCGGCTCCGGCTCCCGACTCTTCGGCCCGC
TGGCATCTGCTTCCCTCCCCTGCCTCGTTTCTCGTCGCCCCTGCTCGCTCCCCCCGGCGCTCGCCCGGGCGCTGTGCTCGCTCCTGGATCGCCAGCCGCG
CAGCCGGGCTCGGCCGGCCGCCCGCGCGCCACTGTGCAGTGGAGTTTGGTGGAATCTCTGCTGACGTCACGTCACTCCCCACACGGAGTAGGAGCAGAGG
GAAGAGAGAGGGATGAGAGGGAGGGAGAGGAGAGAGAGTGCGAGACCGAGCGAGAAAGCTGGAGAGGAGCAGAAAGAAACTGCCAGTGGCGGCTAGATTT
CGGAGGCCCCAGTGCACCCGTGGACTCCTTCGGAACTTGGCACCCTCAGGAGCCCTGCAGTCCTCTCAGGCCCGGCTTTCGGGCGCTTGCCGTGCAGCCG
GAGGCTCGGCTCGCTGGAAATCGCCCCGGGAAGCAGTGGGACGCGGAGACAGCAGCTCTCTCCCGGTAGCCGATAACGGGGAATGGAGACCAACTGCCGC
AAACTGGTGTCGGCGTGTGTGCAATTAGGCGTGCAGCCGGCGGCCGTTGAATGTCTCTTCTCCAAAGACTCCGAAATCAAAAAGGTCGAGTTCACGGACT
CTCCTGAGAGCCGAAAAGAGGCAGCCAGCAGCAAGTTCTTCCCGCGGCAGCATCCTGGCGCCAATGAGAAAGATAAAAGCCAGCAGGGGAAGAATGAGGA
CGTGGGCGCCGAGGACCCGTCTAAGAAGAAGCGGCAAAGGCGGCAGCGGACTCACTTTACCAGCCAGCAGCTCCAGGAGCTGGAGGCCACTTTCCAGAGG
AACCGCTACCCGGACATGTCCACACGCGAAGAAATCGCTGTGTGGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGTCGGGCCAAAT
GGAGAAAGAGGGAGCGCAACCAGCAGGCCGAGCTATGCAAGAATGGCTTCGGGCCGCAGTTCAATGGGCTCATGCAGCCCTACGACGACATGTACCCAGG
CTATTCCTACAACAACTGGGCCGCCAAGGGCCTTACATCCGCCTCCCTATCCACCAAGAGCTTCCCCTTCTTCAACTCTATGAACGTCAACCCCCTGTCA
TCACAGAGCATGTTTTCCCCACCCAACTCTATCTCGTCCATGAGCATGTCGTCCAGCATGGTGCCCTCAGCAGTGACAGGCGTCCCGGGCTCCAGTCTCA
ACAGCCTGAATAACTTGAACAACCTGAGTAGCCCGTCGCTGAATTCCGCGGTGCCGACGCCTGCCTGTCCTTACGCGCCGCCGACTCCTCCGTATGTTTA
TAGGGACACGTGTAACTCGAGCCTGGCCAGCCTGAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGCCTCCAACCTG
AGTGCTTGCCAGTATGCAGTGGACCGGCCCGTGTGAGCCGCACCCACAGCGCCGGGATCCTAGGACCTTGCCGGATGGGGCAACTCCGCCCTTGAAAGAC
TGGGAATTATGCTAGAAGGTCGTGGGCACTAAAGAAAGGGAGAGAAAGAGAAGCTATATAGAGAAAAGGAAACCACTGAATCAAAGAGAGAGCTCCTTTG
ATTTCAAAGGGATGTCCTCAGTGTCTGACATCTTTCACTACAAGTATTTCTAACAGTTGCAAGGACACATACACAAACAAATGTTTGACTGGATATGACA
TTTTAACATTACTATAAGCTTGTTATTTTTTAAGTTTAGCATTGTTAACATTTAAATGACTGAAAGGATGTATATATATCGAAATGTCAAATTAATTTTA
TAAAAGCAGTTGTTAGTAATATCACAACAGTGTTTTTAAAGGTTAGGCTTTAAAATAAAGCATGTTATACAGAAGCGATTAGGATTTTTCGCTTGCGAGC
AAGGGAGTGTATATACTAAATGCCACACTGTATGTTTCTAACATATTATTATTATTATAAAAAATGTGTGAATATCAGTTTTAGAATAGTTTCTCTGGTG
GATGCAATGATGTTTCTGAAACTGCTATGTACAACCTACCCTGTGTATAACATTTCGTACAATATTATTGTTTTACTTTTCAGCAAATATGAAACAAATG
TGTTTTATTTCATGGGAGTAAAATATACTGCATACAAA
  • refseq_PITX2.F2 (forward primer)
  • refseq_PITX2.R2 (reverse primer)
  • Single exon skipping

Frame 0

WESVLLLLGWLLSAPPGPLSFRSPGSGSRLFGP
LASASLPCLVSRRPCSLPPALARALCSLLDRQPR
SRARPAARAPLCSGVWWNLC*RHVTPHTE*EQR
EERGMRGRERRESARPSEKAGEEQKETASGG*I
SEAPVHPWTPSELGTLRSPAVLSGPAFGRLPCSR
RLGSLEIAPGSSGTRRQQLSPGSR*RGMETNCR
KLVSACVQLGVQPAAVECLFSKDSEIKKVEFTD
SPESRKEAASSKFFPRQHPGANEKDKSQQGKNED
VGAEDPSKKKRQRRQRTHFTSQQLQELEATFQR
NRYPDMSTREEIAVWTNLTEARVRVWFKNRRAK
WRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPG
YSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLS
SQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSL
NSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVY
RDTCNSSLASLRLKAKQHSSFGYASVQNPASNL
SACQYAVDRPV*AAPTAPGS*DLAGWGNSALER
LGIMLEGRGH*RKGEKEKLYREKETTESKRELL*
FQRDVLSV*HLSLQVFLTVARTHTQTNV*LDMT
F*HYYKLVIF*V*HC*HLND*KDVYISKCQINF
IKAVVSNITTVFLKVRL*NKACYTEAIRIFRLRA
RECIY*MPHCMFLTYYYYYKKCVNISFRIVSLV
DAMMFLKLLCTTYPVYNISYNIIVLLFSKYETN
VFYFMGVKYTAYK

Frame 1

GSPCSCSSVGS*VPRQVPSPFALPAPAPDSSAR
WHLLPSPASFLVAPARSPRRSPGRCARSWIASR
AAGLGRPPARHCAVEFGGISADVTSLPTRSRSRG
KREG*EGGRGERVRDRARKLERSRKKLPVAARF
RRPQCTRGLLRNLAPSGALQSSQARLSGACRAA
GGSARWKSPREAVGRGDSSSLPVADNGEWRPTAA
NWCRRVCN*ACSRRPLNVSSPKTPKSKRSSSRT
LLRAEKRQPAASSSRGSILAPMRKIKASRGRMR
TWAPRTRLRRSGKGGSGLTLPASSSRSWRPLSRG
TATRTCPHAKKSLCGPTLRKPESGFGSRIVGPN
GERGSATSRPSYARMASGRSSMGSCSPTTTCTQ
AIPTTTGPPRALHPPPYPPRASPSSTL*TSTPCH
HRACFPHPTLSRP*ACRPAWCPQQ*QASRAPVS
TA*IT*TT*VARR*IPRCRRLPVLTRRRLLRMF
IGTRVTRAWPA*D*KQSSTPASATPACRTRPPT*
VLASMQWTGPCEPHPQRRDPRTLPDGATPPLKD
WELC*KVVGTKERERKRSYIEKRKPLNQRESSF
DFKGMSSVSDIFHYKYF*QLQGHIHKQMFDWI*H
FNITISLLFFKFSIVNI*MTERMYIYRNVKLIL
*KQLLVISQQCF*RLGFKIKHVIQKRLGFFACE
QGSVYTKCHTVCF*HIIIIIKNV*ISVLE*FLWW
MQ*CF*NCYVQPTLCITFRTILLFYFSANMKQM
CFISWE*NILHT

Frame 2

GVRAPAPRLAPKCPARSPLLSLSRLRLPTLRPA
GICFPPLPRFSSPLLAPPGARPGAVLAPGSPAA
QPGSAGRPRATVQWSLVESLLTSRHSPHGVGAE
GRERDEREGEERECETERESWRGAERNCQWRLDF
GGPSAPVDSFGTWHPQEPCSPLRPGFRALAVQP
EARLAGNRPGKQWDAETAALSR*PITGNGDQLP
QTGVGVCAIRRAAGGR*MSLLQRLRNQKGRVHGL
S*EPKRGSQQQVLPAAASWRQ*ER*KPAGEE*G
RGRRGPV*EEAAKAAADSLYQPAAPGAGGHFPE
EPLPGHVHTRRNRCVDQPYGSPSPGLVQESSGQM
EKEGAQPAGRAMQEWLRAAVQWAHAALRRHVPR
LFLQQLGRQGPYIRLPIHQELPLLQLYERQPPV
ITEHVFPTQLYLVHEHVVQHGALSSDRRPGLQSQ
QPE*LEQPE*PVAEFRGADACLSLRAADSSVCL
*GHV*LEPGQPETESKAALQLRLRQRAEPGLQP
ECLPVCSGPARVSRTHSAGILGPCRMGQLRP*KT
GNYARRSWALKKGREREAI*RKGNH*IKERAPL
ISKGCPQCLTSFTTSISNSCKDTYTNKCLTGYD
ILTLL*ACYFLSLALLTFK*LKGCIYIEMSN*FY
KSSC**YHNSVFKG*ALK*SMLYRSD*DFSLAS
KGVYILNATLYVSNILLLL*KMCEYQF*NSFSG
GCNDVSETAMYNLPCV*HFVQYYCFTFQQI*NKC
VLFHGSKIYCIQ

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