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Sequences

Sequence NM_004103

GCAGTTCCCGCCTCCTCAGGTCCGGGCGGGTCCCTGGCCGGGGTAGCACGGAAGGGTCTCCCAGGCGGCGTAGTAGGGCTTCCGTGTTACTGGAAACCTA CTTCCGGCTGCAAATGGGAAAAGGAGCCTCTACCTTAACCAATCCCCGGGAACCTCAGGCCCGCGGATGGGAGAAACCAGAGATGCCAACTTCCTGCTTC CGAAGTAGTGGCTGGGTCTTAAAGCACCGATGTTTCTGCATTGAATAGCCCTGGAAGACAGAATTCTTGTCTCTCCAAAGATCTGGCAGACTTATTCCCA ATTATAAGAGATCCTTGCAAAGGAGGTGTCTTGCTTCACCTTCACCTTCCACTGTGATTGTAAGTTTTCTGAGGCCTCGCCAGCCATGCGGAACTGAATA GGACTAGCAACTTCATAGACGGTTGTGAAGACAAGCTAGACGGCAGATGAAAGTTCTTGGCACAGAGTGAACACTTGATAAACTATATGGCAGCCACAGC CTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACTTACCTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCG CGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGCCTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCC GGCCTGAAGGCCCTGCAGAGCCCATGGTGGTGGTACCAGTAGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAA TCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAGACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATC CGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGAAGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGT ATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCTTCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCT GCTCTATTTTTACCAACAGCTCCGGAACGACTACATGCAGCGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGG CGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGAAGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGA TGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGATCCAGCAGACCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTT CAACACTCTCGCCGGCTTCGCCAACATCGACCAGGAGACCTACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTCATTGGCCCTAAA GGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCTGCCTGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGG CAGTACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTTGTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTA CTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATCCATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTG GAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAGAGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGT ATGGCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCTTGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCTACACAAATCACAAAGGGGAGAA AATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACTCTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCG CACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCACCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACA AGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACTGCAGATATGCAAAGCCATGGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGC TGTCCGGAACATCCTGGTGGCCTCCCCTGAGTGTGTGAAGCTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTGTG ACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTAACTTCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGA TCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAACAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCC ACCGGTCCTTTATACCCTCATGACCCGCTGCTGGGACTACGACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAG ATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTCGCTACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCA GCCGACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCTGGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCTGTGTGCCAGCTCTCCTACGCTCAC CAGCCCTATGGAGTATCCATCTCCCGTTAACTCACTGCACACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGGGAGGAGGACTTC ATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGGAGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGG ACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCCCATGGTTTATATGAATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGA GTTCACAGGGCCCCCACAGAAGCCCCCGAGGCTGGGCGCACAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTCAATGTC ATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGCTCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGC GGAAGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTTGCCGTCATCTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGC AGAGCTCATCAACAAGATGCGGCTGGCACAGCAGAACGCCGTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCACACACCCTGGCT GTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCAAGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCC TGCGTCTTCCGCCCCTGCCTGCCATGTACCTCCCCTGCCTTGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCCAAGGGGAGTCACCTTCCCTTGCCA CTTTGCACGACGCCCTCTCCCCACCCCTACCCCTGGCTGTACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGGACACAGGGTGACGGTGA CAAAGATGGCTCAGAGGGGGACTGCTGCTGCCTGGCCACTGCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGGAGGTGTCACATGG TGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGGGAACCAAGCTATTCCTTTCCCTTCCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGA AGCTGGGGAGGAGCTTTGTTTTGGGGGTCAGGCAGCCAGTGAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGAAATTTATTTAATGTGAG TTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGACCTGGGGCACAGTCCAGGAACAAGCTAATTGGGAGTCCAGGCACAGGATGCTGTGTTGTC AACAAACCAAGCATCAGGGGGAAGAAGCAGAGAGATGCGGCCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTT TACTTTCCCTTGCTTTTCCCTCTTTTCTTACTCCTCCTCTTTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCTTTTCTCATGTGTTTGCATAAACA TTCTTTTAACTTCTTTCTATTTGACTTGTGGTTGAATTAAAATTGTCCCATTTGC

refseq_PTK2B.F3 (forward primer)
refseq_PTK2B.R3 (reverse primer)
Single exon skipping

Frame 0

AVPASSGPGGSLAGVARKGLPGGVVGLPCYWKP TSGCKWEKEPLP*PIPGNLRPADGRNQRCQLPAS EVVAGS*STDVSALNSPGRQNSCLSKDLADLFP IIRDPCKGGVLLHLHLPL*L*VF*GLASHAELN RTSNFIDGCEDKLDGR*KFLAQSEHLINYMAATA SGAVAHLPARPTYLYLPPSRLTWRCPRSSRWSP RLPGTAMCRS*LLPERMSGVSEPLSRVKLGTLR RPEGPAEPMVVVPVDVEKEDVRILKVCFYSNSFN PGKNFKLVKCTVQTEIREIITSILLSGRIGPNI RLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDK YECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTL LYFYQQLRNDYMQRYASKVSEGMALQLGCLELR RFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQ MQENLKPKQFRKMIQQTFQQYASLREEECVMKFF NTLAGFANIDQETYRCELIQGWNITVDLVIGPK GIRQLTSQDAKPTCLAEFKQIRSIRCLPLEEGQ AVLQLGIEGAPQALSIKTSSLAEAENMADLIDGY CRLQGEHQGSLIIHPRKDGEKRNSLPQIPMLNL EARRSHLSESCSIESDIYAEIPDETLRRPGGPQ YGIAREDVVLNRILGEGFFGEVYEGVYTNHKGEK INVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHP HIVKLIGIIEEEPTWIIMELYPYGELGHYLERN KNSLKVLTLVLYSLQICKAMAYLESINCVHRDIA VRNILVASPECVKLGDFGLSRYIEDEDYYKASV TRLPIKWMSPESINFRRFTTASDVWMFAVCMWE ILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCP PVLYTLMTRCWDYDPSDRPRFTELVCSLSDVYQ MEKDIAMEQERNARYRTPKILEPTAFQEPPPKP SRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLT SPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDF IQPSSREEAQQLWEAEKVKMRQILDKQQKQMVE DYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLE FTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNV MELVRAVLELKNELCQLPPEGYVVVVKNVGLTL RKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLA ELINKMRLAQQNAVTSLSEECKRQMLTASHTLA VDAKNLLDAVDQAKVLANLAHPPAE*RRVGATC LRLPPLPAMYLPCLAVGHVGLPGGRPRGVTFPCH FARRPLPTPTPGCTAQAAAGQRGLWAMDTG*R* QRWLRGGLLLPGHCSLSQPGPCRGLLGVGRCHM VPLALYMDMAGRFGNQAIPFPSSSALRCPLMHRE AGEELCFGGQAASEMRDGPGILVQCILKFI*CE FGLD*QHVPS*GRTWGTVQEQANWESRHRMLCC QQTKHQGEEAERCGQDRTLGQIRSLPAPLSLSSF TFPCFSLFSYSSSFSPQPPFSSAPFFSHVFA*T FF*LLSI*LVVELKLSHL

Frame 1

QFPPPQVRAGPWPG*HGRVSQAA**GFRVTGNL LPAANGKRSLYLNQSPGTSGPRMGETRDANFLL PK*WLGLKAPMFLH*IALEDRILVSPKIWQTYSQ L*EILAKEVSCFTFTFHCDCKFSEASPAMRN*I GLATS*TVVKTS*TADESSWHRVNT**TIWQPQ PPEPLHTYLPGRLTCTCRRPGSPGGARGVVAGVR ASLGLQCADLSCCLRGCLGCPSP*VE*SWARYA GLKALQSPWWWYQ*MWKRRTCVSSRSASIATAS ILGKTSNWSNALSRRRSGRSSPPSC*AGGSGPTS GWLSAMG*G*ST*SPMRSTGCTHR*RWVRCRTS MSVCTWKPSGGMTFKSATCQKTSWRA*RRTGPR CSIFTNSSGTTTCSATPARSARAWPCSWAAWSSG GSSRICPTMHLTRSPTSSS*KRKWGWTCFSQSR CRRT*SPNSSGR*SSRPSSSTPRSGRRSAS*SS STLSPASPTSTRRPTAVNSFKDGTLLWTWSLALK GSAS*LVRTQSPPAWPSSSRSGPSGASRWRRAR QYFSWALKVPPRPCPSKPHP*QRLRTWLTS*TA TAGCRVSTKALSSSILGKMVRSGTACPRSPC*TW RPGGPTSQRAAA*SQTSTQRFPTKPCEGPEVHS MALPVKMWS*IVFLGKAFLGRSMKVSTQITKGR KSM*LSRPARKTALWTTRRSS*ARQ*S*RTSTTR TS*S*SASLKRSPPGSSWNCIPMGSWATTWSGT RTP*RCSPSCCTHCRYAKPWPTWRASTACTGTL LSGTSWWPPLSV*SWGTLVFPGTLRTRTITKPL* LVSPSNGCPQSPLTSDASRQPVTSGCSPCACGR S*ALGSSPSSGWRTRMSSGCWRKETGCPSLISV HRSFIPS*PAAGTTTPVTGPASPSWCAASVTFIR WRRTLPWSKRGMLATEPPKSWSPQPSRNPHPSP ADLSTDPLRKPTSWLQSCSSRFLRVCVPALLRS PALWSIHLPLTHCTPHLSTGTMSSNATACGRRTS SNPAAEKRPSSCGRLKRSKCGKSWTNSRSRWWR TTSGSGRRRSPWTPWFI*MISPH*RQRRRSATW SSQGPHRSPRGWAHSPSSPQLTWTGLMTWCTSMS WSWCGPCWSSRMSSVSCPPRATWWW*RMWG*PC GSSSGAWMISCLPCRHLHGQRSRAPRNCSTKTW QSSSTRCGWHSRTP*PP*VRSARGRC*RLHTPWL WTPRTCSTLWTRPRFWPIWPTHLQSDGGWGPPA CVFRPCLPCTSPALLLVMWVFQGEGQGESPSLA TLHDALSPPLPLAVLLRLQLDRGDSGLWTQGDGD KDGSEGDCCCLATAP*ASLVHAGGSWGWGGVTW CP*LYIWTWQADLGTKLFLSLPLRPSDVP*CTE KLGRSFVLGVRQPVR*GMGLAFLYSVY*NLFNVS LVWTDSMCPPEGGPGAQSRNKLIGSPGTGCCVV NKPSIRGKKQRDAAKIGPWAKSALFLPLFLFLP LLSLAFPSFLTPPLSLPNPHSHLHPSFLMCLHKH SFNFFLFDLWLN*NCPIC

Frame 2

SSRLLRSGRVPGRGSTEGSPRRRSRASVLLETY FRLQMGKGASTLTNPREPQARGWEKPEMPTSCF RSSGWVLKHRCFCIE*PWKTEFLSLQRSGRLIP NYKRSLQRRCLASPSPSTVIVSFLRPRQPCGTE* D*QLHRRL*RQARRQMKVLGTE*TLDKLYGSHS LRSRCTPTCPADLPVLAAVPAHLAVPEE*SLES APPWDCNVPILAAA*EDVWGVRAPESSKVGHVTP A*RPCRAHGGGTSRCGKGGRAYPQGLLL*QQLQ SWEKLQTGQMHCPDGDPGDHHLHPAERADRAQH PVG*VLWAEAEAHEVR*DPLAAPTDDGG*GAGQV *VSARGSRVEV*PSNPLLARRLHGEPEGGQDHA ALFLPTAPERLHAALRQQGQRGHGPAAGLPGAQ AVLQGYAPQCT*QEVQLRAPRKGSGAGLVFPKAD AGELKAQTVPEDDPADLPAVRLAQGGGVRHEVL QHSRRLRQHRPGDLPL*THSRMEHYCGPGHWP* RDPPAD*SGRKAHLPGRVQADQVHQVPPAGGGPG STSAGH*RCPPGLVHQNLIPSRG*EHG*PHRRL LPAAG*APRLSHHPS*ERW*EAEQPAPDPHAKP GGPAVPPLRELQHRVRHLRRDSRRNPAKARRSTV WHCP*RCGPESYSWGRLFWGGL*RCLHKSQRGE NQCSCQDLQERLHSGQQGEVHERGSDHEEPRPP AHREADRHH*RGAHLDHHGIVSLWGAGPLPGAEQ ELPEGAHPRAVLTADMQSHGLPGEHQLRAQGHC CPEHPGGLP*VCEAGGLWSFPVH*GRGLLQSLC DSSPHQMDVPRVH*LPTLHDSQ*RLDVRRVHVGD PELWEAALLLAGEQGCHRGAGERRPAAQA*SLS TGPLYPHDPLLGLRPQ*PAPLHRAGVQPQ*RLS DGEGHCHGAREECSLPNPQNLGAHSLPGTPTQAQ PT*VQTPSANQPPGSKAAVPGS*GSVCQLSYAH QPYGVSISR*LTAHPTSPPAQCLQTPQHAGGGL HPTQQPRRGPAAVGG*KGQNAANPGQTAEADGGG LPVAQAGGEVPGPHGLYE**VPIDAREGGRLPG VHRAPTEAPEAGRTVHPAHS*PGPD**PGVPQC HGAGAGRAGAQE*ALSAAPRGLRGGGEECGADPA EAHRERG*SPAFLAVIFTDRDRGHPETAQQRPG RAHQQDAAGTAERRDLPK*GVQEADADGFTHPG CGRQEPARRCGPGQGSGQSGPPTCRVTEGGGHLP ASSAPACHVPPLPCCWSCGSSRGKAKGSHLPLP LCTTPSPHPYPWLYCSGCSWTEGTLGYGHRVTV TKMAQRGTAAAWPLLPKPAWSMQGAPGGGEVSHG APSFIYGHGRPIWEPSYSFPFLFGPQMSLDAQR SWGGALFWGSGSQ*DEGWAWHSCTVYIEIYLM* VWSGLTACALLREDLGHSPGTS*LGVQAQDAVLS TNQASGGRSREMRPR*DLGPNPLSSCPSFSFFL YFPLLFPLFLLLLFLSPTPILICTLLFSCVCIN ILLTSFYLTCG*IKIVPF

Sequence NM_173174

GCAGTTCCCGCCTCCTCAGGTCCGGGCGGGTCCCTGGCCGGGGTAGCACGGAAGGGTCTCCCAGGCGGCGTAGTAGGGCTTCCGTGTTACTGGAAACCTA CTTCCGGCTGCAAATGGGAAAAGGAGCCTCTACCTTAACCAATCCCCGGGAACCTCAGGCCCGCGGATGGGAGAAACCAGAGATGCCAACTTCCTGCTTC CGAAGTAGTGGCTGGGTCTTAAAGCACCGATGTTTCTGCATTGAATAGCCCTGGAAGACAGAATTCTTGTCTCTCCAAAGATCTGGCAGACTTATTCCCA ATTATAAGAGATCCTTGCAAAGGAGGTGTCTTGCTTCACCTTCACCTTCCACTGTGATTGTAAGTTTTCTGAGGCCTCGCCAGCCATGCGGAACTGAATA GGACTAGCAACTTCATAGACGGTTGTGAAGACAAGCTAGACGGCAGATGAAAGTTCTTGGCACAGAGTGAACACTTGATAAACTATATGGCAGGGAGGGC TGGAACGGGGCTTGTTTGAAGAGCAATATGAGCCAGGGTTATAGACCTGAGTTTGGGGTAAGAGTGGAACATCCAAATGGAAGAGTCCAGCAGCTGAAAG GACATTTGTTCAAAGGCCTTTTTCATTACAGTTTTCTCCTTCTTCTGATCCAGCCACAGCCTCCGGAGCCGTTGCACACCTACCTGCCCGGCCGACTTAC CTGTACTTGCCGCCGTCCCGGCTCACCTGGCGGTGCCCGAGGAGTAGTCGCTGGAGTCCGCGCCTCCCTGGGACTGCAATGTGCCGATCTTAGCTGCTGC CTGAGAGGATGTCTGGGGTGTCCGAGCCCCTGAGTCGAGTAAAGTTGGGCACGTTACGCCGGCCTGAAGGCCCTGCAGAGCCCATGGTGGTGGTACCAGT AGATGTGGAAAAGGAGGACGTGCGTATCCTCAAGGTCTGCTTCTATAGCAACAGCTTCAATCCTGGGAAAAACTTCAAACTGGTCAAATGCACTGTCCAG ACGGAGATCCGGGAGATCATCACCTCCATCCTGCTGAGCGGGCGGATCGGGCCCAACATCCGGTTGGCTGAGTGCTATGGGCTGAGGCTGAAGCACATGA AGTCCGATGAGATCCACTGGCTGCACCCACAGATGACGGTGGGTGAGGTGCAGGACAAGTATGAGTGTCTGCACGTGGAAGCCGAGTGGAGGTATGACCT TCAAATCCGCTACTTGCCAGAAGACTTCATGGAGAGCCTGAAGGAGGACAGGACCACGCTGCTCTATTTTTACCAACAGCTCCGGAACGACTACATGCAG CGCTACGCCAGCAAGGTCAGCGAGGGCATGGCCCTGCAGCTGGGCTGCCTGGAGCTCAGGCGGTTCTTCAAGGATATGCCCCACAATGCACTTGACAAGA AGTCCAACTTCGAGCTCCTAGAAAAGGAAGTGGGGCTGGACTTGTTTTTCCCAAAGCAGATGCAGGAGAACTTAAAGCCCAAACAGTTCCGGAAGATGAT CCAGCAGACCTTCCAGCAGTACGCCTCGCTCAGGGAGGAGGAGTGCGTCATGAAGTTCTTCAACACTCTCGCCGGCTTCGCCAACATCGACCAGGAGACC TACCGCTGTGAACTCATTCAAGGATGGAACATTACTGTGGACCTGGTCATTGGCCCTAAAGGGATCCGCCAGCTGACTAGTCAGGACGCAAAGCCCACCT GCCTGGCCGAGTTCAAGCAGATCAGGTCCATCAGGTGCCTCCCGCTGGAGGAGGGCCAGGCAGTACTTCAGCTGGGCATTGAAGGTGCCCCCCAGGCCTT GTCCATCAAAACCTCATCCCTAGCAGAGGCTGAGAACATGGCTGACCTCATAGACGGCTACTGCCGGCTGCAGGGTGAGCACCAAGGCTCTCTCATCATC CATCCTAGGAAAGATGGTGAGAAGCGGAACAGCCTGCCCCAGATCCCCATGCTAAACCTGGAGGCCCGGCGGTCCCACCTCTCAGAGAGCTGCAGCATAG AGTCAGACATCTACGCAGAGATTCCCGACGAAACCCTGCGAAGGCCCGGAGGTCCACAGTATGGCATTGCCCGTGAAGATGTGGTCCTGAATCGTATTCT TGGGGAAGGCTTTTTTGGGGAGGTCTATGAAGGTGTCTACACAAATCACAAAGGGGAGAAAATCAATGTAGCTGTCAAGACCTGCAAGAAAGACTGCACT CTGGACAACAAGGAGAAGTTCATGAGCGAGGCAGTGATCATGAAGAACCTCGACCACCCGCACATCGTGAAGCTGATCGGCATCATTGAAGAGGAGCCCA CCTGGATCATCATGGAATTGTATCCCTATGGGGAGCTGGGCCACTACCTGGAGCGGAACAAGAACTCCCTGAAGGTGCTCACCCTCGTGCTGTACTCACT GCAGATATGCAAAGCCATGGCCTACCTGGAGAGCATCAACTGCGTGCACAGGGACATTGCTGTCCGGAACATCCTGGTGGCCTCCCCTGAGTGTGTGAAG CTGGGGGACTTTGGTCTTTCCCGGTACATTGAGGACGAGGACTATTACAAAGCCTCTGTGACTCGTCTCCCCATCAAATGGATGTCCCCAGAGTCCATTA ACTTCCGACGCTTCACGACAGCCAGTGACGTCTGGATGTTCGCCGTGTGCATGTGGGAGATCCTGAGCTTTGGGAAGCAGCCCTTCTTCTGGCTGGAGAA CAAGGATGTCATCGGGGTGCTGGAGAAAGGAGACCGGCTGCCCAAGCCTGATCTCTGTCCACCGGTCCTTTATACCCTCATGACCCGCTGCTGGGACTAC GACCCCAGTGACCGGCCCCGCTTCACCGAGCTGGTGTGCAGCCTCAGTGACGTTTATCAGATGGAGAAGGACATTGCCATGGAGCAAGAGAGGAATGCTC GCTACCGAACCCCCAAAATCTTGGAGCCCACAGCCTTCCAGGAACCCCCACCCAAGCCCAGCCGACCTAAGTACAGACCCCCTCCGCAAACCAACCTCCT GGCTCCAAAGCTGCAGTTCCAGGTTCCTGAGGGTCTGTGTGCCAGCTCTCCTACGCTCACCAGCCCTATGGAGTATCCATCTCCCGTTAACTCACTGCAC ACCCCACCTCTCCACCGGCACAATGTCTTCAAACGCCACAGCATGCGGGAGGAGGACTTCATCCAACCCAGCAGCCGAGAAGAGGCCCAGCAGCTGTGGG AGGCTGAAAAGGTCAAAATGCGGCAAATCCTGGACAAACAGCAGAAGCAGATGGTGGAGGACTACCAGTGGCTCAGGCAGGAGGAGAAGTCCCTGGACCC CATGGTTTATATGAATGATAAGTCCCCATTGACGCCAGAGAAGGAGGTCGGCTACCTGGAGTTCACAGGGCCCCCACAGAAGCCCCCGAGGCTGGGCGCA CAGTCCATCCAGCCCACAGCTAACCTGGACCGGACTGATGACCTGGTGTACCTCAATGTCATGGAGCTGGTGCGGGCCGTGCTGGAGCTCAAGAATGAGC TCTGTCAGCTGCCCCCCGAGGGCTACGTGGTGGTGGTGAAGAATGTGGGGCTGACCCTGCGGAAGCTCATCGGGAGCGTGGATGATCTCCTGCCTTCCTT GCCGTCATCTTCACGGACAGAGATCGAGGGCACCCAGAAACTGCTCAACAAAGACCTGGCAGAGCTCATCAACAAGATGCGGCTGGCACAGCAGAACGCC GTGACCTCCCTAAGTGAGGAGTGCAAGAGGCAGATGCTGACGGCTTCACACACCCTGGCTGTGGACGCCAAGAACCTGCTCGACGCTGTGGACCAGGCCA AGGTTCTGGCCAATCTGGCCCACCCACCTGCAGAGTGACGGAGGGTGGGGGCCACCTGCCTGCGTCTTCCGCCCCTGCCTGCCATGTACCTCCCCTGCCT TGCTGTTGGTCATGTGGGTCTTCCAGGGGGAAGGCCAAGGGGAGTCACCTTCCCTTGCCACTTTGCACGACGCCCTCTCCCCACCCCTACCCCTGGCTGT ACTGCTCAGGCTGCAGCTGGACAGAGGGGACTCTGGGCTATGGACACAGGGTGACGGTGACAAAGATGGCTCAGAGGGGGACTGCTGCTGCCTGGCCACT GCTCCCTAAGCCAGCCTGGTCCATGCAGGGGGCTCCTGGGGGTGGGGAGGTGTCACATGGTGCCCCTAGCTTTATATATGGACATGGCAGGCCGATTTGG GAACCAAGCTATTCCTTTCCCTTCCTCTTCGGCCCTCAGATGTCCCTTGATGCACAGAGAAGCTGGGGAGGAGCTTTGTTTTGGGGGTCAGGCAGCCAGT GAGATGAGGGATGGGCCTGGCATTCTTGTACAGTGTATATTGAAATTTATTTAATGTGAGTTTGGTCTGGACTGACAGCATGTGCCCTCCTGAGGGAGGA CCTGGGGCACAGTCCAGGAACAAGCTAATTGGGAGTCCAGGCACAGGATGCTGTGTTGTCAACAAACCAAGCATCAGGGGGAAGAAGCAGAGAGATGCGG CCAAGATAGGACCTTGGGCCAAATCCGCTCTCTTCCTGCCCCTCTTTCTCTTTCTTCCTTTACTTTCCCTTGCTTTTCCCTCTTTTCTTACTCCTCCTCT TTCTCTCCCCAACCCCCATTCTCATCTGCACCCTTCTTTTCTCATGTGTTTGCATAAACATTCTTTTAACTTCTTTCTATTTGACTTGTGGTTGAATTAA AATTGTCCCATTTGC

refseq_PTK2B.F3 (forward primer)
refseq_PTK2B.R3 (reverse primer)
Single exon skipping

Frame 0

AVPASSGPGGSLAGVARKGLPGGVVGLPCYWKP TSGCKWEKEPLP*PIPGNLRPADGRNQRCQLPAS EVVAGS*STDVSALNSPGRQNSCLSKDLADLFP IIRDPCKGGVLLHLHLPL*L*VF*GLASHAELN RTSNFIDGCEDKLDGR*KFLAQSEHLINYMAGRA GTGLV*RAI*ARVIDLSLG*EWNIQMEESSS*K DICSKAFFITVFSFF*SSHSLRSRCTPTCPADL PVLAAVPAHLAVPEE*SLESAPPWDCNVPILAAA *EDVWGVRAPESSKVGHVTPA*RPCRAHGGGTS RCGKGGRAYPQGLLL*QQLQSWEKLQTGQMHCP DGDPGDHHLHPAERADRAQHPVG*VLWAEAEAHE VR*DPLAAPTDDGG*GAGQV*VSARGSRVEV*P SNPLLARRLHGEPEGGQDHAALFLPTAPERLHA ALRQQGQRGHGPAAGLPGAQAVLQGYAPQCT*QE VQLRAPRKGSGAGLVFPKADAGELKAQTVPEDD PADLPAVRLAQGGGVRHEVLQHSRRLRQHRPGD LPL*THSRMEHYCGPGHWP*RDPPAD*SGRKAHL PGRVQADQVHQVPPAGGGPGSTSAGH*RCPPGL VHQNLIPSRG*EHG*PHRRLLPAAG*APRLSHH PS*ERW*EAEQPAPDPHAKPGGPAVPPLRELQHR VRHLRRDSRRNPAKARRSTVWHCP*RCGPESYS WGRLFWGGL*RCLHKSQRGENQCSCQDLQERLH SGQQGEVHERGSDHEEPRPPAHREADRHH*RGAH LDHHGIVSLWGAGPLPGAEQELPEGAHPRAVLT ADMQSHGLPGEHQLRAQGHCCPEHPGGLP*VCE AGGLWSFPVH*GRGLLQSLCDSSPHQMDVPRVH* LPTLHDSQ*RLDVRRVHVGDPELWEAALLLAGE QGCHRGAGERRPAAQA*SLSTGPLYPHDPLLGL RPQ*PAPLHRAGVQPQ*RLSDGEGHCHGAREECS LPNPQNLGAHSLPGTPTQAQPT*VQTPSANQPP GSKAAVPGS*GSVCQLSYAHQPYGVSISR*LTA HPTSPPAQCLQTPQHAGGGLHPTQQPRRGPAAVG G*KGQNAANPGQTAEADGGGLPVAQAGGEVPGP HGLYE**VPIDAREGGRLPGVHRAPTEAPEAGR TVHPAHS*PGPD**PGVPQCHGAGAGRAGAQE*A LSAAPRGLRGGGEECGADPAEAHRERG*SPAFL AVIFTDRDRGHPETAQQRPGRAHQQDAAGTAER RDLPK*GVQEADADGFTHPGCGRQEPARRCGPGQ GSGQSGPPTCRVTEGGGHLPASSAPACHVPPLP CCWSCGSSRGKAKGSHLPLPLCTTPSPHPYPWL YCSGCSWTEGTLGYGHRVTVTKMAQRGTAAAWPL LPKPAWSMQGAPGGGEVSHGAPSFIYGHGRPIW EPSYSFPFLFGPQMSLDAQRSWGGALFWGSGSQ *DEGWAWHSCTVYIEIYLM*VWSGLTACALLRED LGHSPGTS*LGVQAQDAVLSTNQASGGRSREMR PR*DLGPNPLSSCPSFSFFLYFPLLFPLFLLLL FLSPTPILICTLLFSCVCINILLTSFYLTCG*IK IVPF

Frame 1

QFPPPQVRAGPWPG*HGRVSQAA**GFRVTGNL LPAANGKRSLYLNQSPGTSGPRMGETRDANFLL PK*WLGLKAPMFLH*IALEDRILVSPKIWQTYSQ L*EILAKEVSCFTFTFHCDCKFSEASPAMRN*I GLATS*TVVKTS*TADESSWHRVNT**TIWQGG LERGLFEEQYEPGL*T*VWGKSGTSKWKSPAAER TFVQRPFSLQFSPSSDPATASGAVAHLPARPTY LYLPPSRLTWRCPRSSRWSPRLPGTAMCRS*LL PERMSGVSEPLSRVKLGTLRRPEGPAEPMVVVPV DVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQ TEIREIITSILLSGRIGPNIRLAECYGLRLKHM KSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDL QIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQ RYASKVSEGMALQLGCLELRRFFKDMPHNALDK KSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMI QQTFQQYASLREEECVMKFFNTLAGFANIDQET YRCELIQGWNITVDLVIGPKGIRQLTSQDAKPT CLAEFKQIRSIRCLPLEEGQAVLQLGIEGAPQAL SIKTSSLAEAENMADLIDGYCRLQGEHQGSLII HPRKDGEKRNSLPQIPMLNLEARRSHLSESCSI ESDIYAEIPDETLRRPGGPQYGIAREDVVLNRIL GEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCT LDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEP TWIIMELYPYGELGHYLERNKNSLKVLTLVLYSL QICKAMAYLESINCVHRDIAVRNILVASPECVK LGDFGLSRYIEDEDYYKASVTRLPIKWMSPESI NFRRFTTASDVWMFAVCMWEILSFGKQPFFWLEN KDVIGVLEKGDRLPKPDLCPPVLYTLMTRCWDY DPSDRPRFTELVCSLSDVYQMEKDIAMEQERNA RYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLL APKLQFQVPEGLCASSPTLTSPMEYPSPVNSLH TPPLHRHNVFKRHSMREEDFIQPSSREEAQQLW EAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDP MVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGA QSIQPTANLDRTDDLVYLNVMELVRAVLELKNE LCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSL PSSSRTEIEGTQKLLNKDLAELINKMRLAQQNA VTSLSEECKRQMLTASHTLAVDAKNLLDAVDQA KVLANLAHPPAE*RRVGATCLRLPPLPAMYLPCL AVGHVGLPGGRPRGVTFPCHFARRPLPTPTPGC TAQAAAGQRGLWAMDTG*R*QRWLRGGLLLPGH CSLSQPGPCRGLLGVGRCHMVPLALYMDMAGRFG NQAIPFPSSSALRCPLMHREAGEELCFGGQAAS EMRDGPGILVQCILKFI*CEFGLD*QHVPS*GR TWGTVQEQANWESRHRMLCCQQTKHQGEEAERCG QDRTLGQIRSLPAPLSLSSFTFPCFSLFSYSSS FSPQPPFSSAPFFSHVFA*TFF*LLSI*LVVEL KLSHL

Frame 2

SSRLLRSGRVPGRGSTEGSPRRRSRASVLLETY FRLQMGKGASTLTNPREPQARGWEKPEMPTSCF RSSGWVLKHRCFCIE*PWKTEFLSLQRSGRLIP NYKRSLQRRCLASPSPSTVIVSFLRPRQPCGTE* D*QLHRRL*RQARRQMKVLGTE*TLDKLYGREG WNGACLKSNMSQGYRPEFGVRVEHPNGRVQQLK GHLFKGLFHYSFLLLLIQPQPPEPLHTYLPGRLT CTCRRPGSPGGARGVVAGVRASLGLQCADLSCC LRGCLGCPSP*VE*SWARYAGLKALQSPWWWYQ *MWKRRTCVSSRSASIATASILGKTSNWSNALSR RRSGRSSPPSC*AGGSGPTSGWLSAMG*G*ST* SPMRSTGCTHR*RWVRCRTSMSVCTWKPSGGMT FKSATCQKTSWRA*RRTGPRCSIFTNSSGTTTCS ATPARSARAWPCSWAAWSSGGSSRICPTMHLTR SPTSSS*KRKWGWTCFSQSRCRRT*SPNSSGR* SSRPSSSTPRSGRRSAS*SSSTLSPASPTSTRRP TAVNSFKDGTLLWTWSLALKGSAS*LVRTQSPP AWPSSSRSGPSGASRWRRARQYFSWALKVPPRP CPSKPHP*QRLRTWLTS*TATAGCRVSTKALSSS ILGKMVRSGTACPRSPC*TWRPGGPTSQRAAA* SQTSTQRFPTKPCEGPEVHSMALPVKMWS*IVF LGKAFLGRSMKVSTQITKGRKSM*LSRPARKTAL WTTRRSS*ARQ*S*RTSTTRTS*S*SASLKRSP PGSSWNCIPMGSWATTWSGTRTP*RCSPSCCTH CRYAKPWPTWRASTACTGTLLSGTSWWPPLSV*S WGTLVFPGTLRTRTITKPL*LVSPSNGCPQSPL TSDASRQPVTSGCSPCACGRS*ALGSSPSSGWR TRMSSGCWRKETGCPSLISVHRSFIPS*PAAGTT TPVTGPASPSWCAASVTFIRWRRTLPWSKRGML ATEPPKSWSPQPSRNPHPSPADLSTDPLRKPTS WLQSCSSRFLRVCVPALLRSPALWSIHLPLTHCT PHLSTGTMSSNATACGRRTSSNPAAEKRPSSCG RLKRSKCGKSWTNSRSRWWRTTSGSGRRRSPWT PWFI*MISPH*RQRRRSATWSSQGPHRSPRGWAH SPSSPQLTWTGLMTWCTSMSWSWCGPCWSSRMS SVSCPPRATWWW*RMWG*PCGSSSGAWMISCLP CRHLHGQRSRAPRNCSTKTWQSSSTRCGWHSRTP *PP*VRSARGRC*RLHTPWLWTPRTCSTLWTRP RFWPIWPTHLQSDGGWGPPACVFRPCLPCTSPA LLLVMWVFQGEGQGESPSLATLHDALSPPLPLAV LLRLQLDRGDSGLWTQGDGDKDGSEGDCCCLAT AP*ASLVHAGGSWGWGGVTWCP*LYIWTWQADL GTKLFLSLPLRPSDVP*CTEKLGRSFVLGVRQPV R*GMGLAFLYSVY*NLFNVSLVWTDSMCPPEGG PGAQSRNKLIGSPGTGCCVVNKPSIRGKKQRDA AKIGPWAKSALFLPLFLFLPLLSLAFPSFLTPPL SLPNPHSHLHPSFLMCLHKHSFNFFLFDLWLN* NCPIC